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BWSpec 4

1. wavelength start nm 1100 wavelength end nm 10 wavelength step nm 4 762099E 02 Irradiance of wavelength 350 0 10 350nm 5 982313E 02 Irradiance of wavelength 350 1 10 360nm 7 276911E 02 Irradiance of wavelength 350 2 10 370nm 8 726646E 02 Irradiance of wavelength 350 3 10 380nm 0 1042587 Irradiance of wavelength 350 4 10 390nm 2 50559 7 Irradiance of wavelength 350 82 10 1070 nm 2 489993 Irradiance of wavelength 350483 10 1080 nm 2 474348 Irradiance of wavelength 350 84 10 1090 nm 2 460015 Irradiance of wavelength 350 85 10 1100 nm S 0 0 0 0000 end flag D300 DAT at c program filesNBWTEKNBWSpec 4 Files Irradiance Source Lamp Data 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 61 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc
2. Dark Compensate Enable Disable Acquire One Spectrum e Click Acquire Continuously to acquire new spectra in a live mode continuously W is updated to the current graph Q Acquire Continuously e Click Acquire Dark to acquire a new spectrum for use as a dark scan Acquire Dark ire a new spectrum for use as a reference scan Acquire Reference 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 9 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 e Click Acquire Overlay to capture a new spectrum and add to the current graph nt acquisition in progress pectra displayed from the current graph display Clear Display Overlay Reset e Click Dark Compensate Enable Disable to turn on or off the electronic dark reference that are available with some detector arrays for automatic dark subtractions based on those available references Dark Compensate Enable Disable 3 1 4 Tools Option Plug in Setup Smoothing Background Removal Derivative Peak Analysis BACISIA Camera Export Data Timeline amp E Me PRES Wavelength Calibration TH 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 10 290020018 G BETA 7 12 12 2011 Copyright 2011
3. C uv C Lab Raw Data Color Save Wavelength lradiance nm uW cm 2 nm 6 76E 04 1 46E 03 2 09E 03 2 80E 03 3 49E 03 3 75E 03 3 97E 03 4 44E 03 4 47E 03 Absolute Irradiance uVW cm 2 nm 550 Wavelength nm The radiometry and chromaticity data is displayed in five categories 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 55 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 e Radiometric Data NN E E a8 Radiometric Data Color Data jale e Color Data 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 56 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc lluminant D amp 5 Observer CIE 1931 10 nm interval from 400 nm to 700 nr 6 196 6 368 Z 25 6 x 0 4066 y 0 4178 z 0 1756 HL 100 000 0 000 Hb 0 000 Luminous Intensity Color Chart xy C uv C Lab Bar Data 4 gt Luminous Intensity 7 1568E 01 med Distance Radiant Unit jmVWV nm Radiant flux 1 2 mW Luminous flux 7 1568E 00 lumen BWSpec 4 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 290020018 G BETA 7 12 12 2011
4. List Panel Absolute Inradiance mrien enm er gz5 La ie 0 020 4 om Wisselength ipm 0 015 P5 Y Fora Tene 010 4 rer y Teamissa Feflection AB olubs rive An ieecnemetera 84 Seectram infec 9 9 Color Measurement 9 9 1 About Color Measurement The color measurement is to characterize an object for example illumination source in terms of its chromaticity and radiometry Chromaticity refers to the quality of the object regardless of its luminance while radiometry measures the radiant energy or electromagnetic energy of the object The color measurement result shown in the Color Chart tab will only be made available when the spectrometer in use covers from at least 400 nm through no shorter than 700 nm spectral range Before performing a color measurement the system should be normalized or irradiance calibrated From the menu bar select Setup Color Setup A dialog box will display where the settings for color measurement can be adjusted e Inside the box for Color Calculation Source a dropdown menu will allow user to choose between 9eT R or Irradiance for the measurement 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 49 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc BWSpec 4 ud Color Measur
5. Your BWSpec 4 Beret Toot Phgi Hip Language MO MAIT Dark Subltracted BWSpec 4 Array Spectrometer Acquisition Software User Manual 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 1 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 TABLE OF CONTENTS 1 MOUCHO 4 20 4 2 1 Operating Systems 2204444424 4 2 2 4 3 Software Main Screen ssssesssseesssssssesssscccoossssssesseeecocssssseessseeeesesssse 4 Menu 5 2 E dia 15 3 3 16 34 Spectrometer Control Panel sevecsevccsssecascxvesccnsesvsaveivaseeesbonsvepsavesdeansoeverstaveseusabonseaetawwses 16 3 5 Spectrometer Info 17 3 0 DI mec 18 SCC UNE ee 18 4 1 Show In Overlay 18 4 2 Show In Sequence 18 4 3 Spectrum Display Setting nii
6. 27 Copyright 2011 B amp W Tek Inc I ok Your Photonics Partner BWSpec 4 e Raw Data Color Save C qth Imadiance lavelen nm uW cm 2 nm Dl 400 00 410 00 1 46 03 420 00 20903 430 00 2 80 3 440 00 3496 13 450 00 e Color Save Color Chart fo C uv C Lab C Bar Color Data Save Show Background Shaw Grid Lines Show Wavelength Values Click Color Data Save to save the color data into BWSpec Color TXT file txt There are three Color Chart Options available for color diagram setting o Show Background o Show Grid Lines o Show Wavelength Values 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 58 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 Appendix A Irradiance Ratio File Data Definition Ratio txt File details and explanation of data Int Time 1 100 Integration Time Data was acquired at for the Ratio File Distance 75 Fiber to Light Source Distance used to create Ratio File Index 0 ZW cm 2 nm Index 1 mW cm 2 nm Index 2 uW cm 2 nm Units will match the Lamp Data file and the units of the irrands_yaxis_min O0 Y axis minimum value based on the start WL for the irradiance range irrands_yaxis_max 4 38087481 104945 Y axis maximum value based on irradiance WL range chos
7. 39 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 60000 50000 40000 30000 Relative Intensity 20000 10000 500 1000 1500 2000 2500 3000 Raman Shift cm 1 e From the menu bar Option Relative Intensity Correction The Relative Intensity Correction window will appear id Create Relative Intensity Correction Ratio File Standard Material Step 1 Tum off light take a dark scan then tum on light take a reference scan Step 2 Select Coefficients of the Certified Polynomial Certified Value Polynomial Coefficient 12 18 Celsius scale _SRM v 0 ATY 2 2 AY v 3 M v 4 AB v 5 ao 1199801 _ 1912010 7 ai 40000 Step 3 Select Reference Data Use Curent Reference Data Use Curent Spectrum Step 4 Select Correction Range Step 5 Set ratio file name Ratio3 NYB Set ratio file path C Program Files BW TEKXBW Spect Ratio M Step 6 Create ratio file Create 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 40 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc 9 6 3 BW I Your Photonics Partner BWSpec 4 Select appropriate standard material for example SRM2241 Select Use Current Spectrum Enter correction range left position right position Set the ratio file name and the
8. 9 7 Reference Materials Correction 9 7 1 About Reference Material Correction In applications such as in diffused reflectance measurements a certified reflectance standard is often used to serve as a reference The R values throughout the measurement range are then calculated by using ratios with respect to the reference in a relative sense With the available certified R values the BWSpec software can calculate the absolute reflectance against the reference The following is a table of a typical certified PTFE type material spectral reflectance white diffuse reflectance standard SRS 99 010 Wavelength Certified R Wavelength Certified R on ae i ug 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 42 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 If we define Rs non correction as the sample percent reflectance at a given wavelength non correction Sample intensity Reference intensity 100 After reference material correction Rs absolute Rs non correction Certified R value 100 where all values are calculated at given wavelengths across the calibrate wavelength range 9 7 2 Generate Reference Material Correction Ratio File This section is for advanced users only e Collect a spectrum using reference materials Select Op
9. Color Chart tab for chromaticity diagram and data display Options of four different CIE color space are available e CIE xyY color space 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 53 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner l BWSpec 4 Spectrum Color Chart e CIE Luv space Color Chart uv Lab Radiometric Data Color Data 4 gt Spectrum Color Chart Ev Lux x CCT Dominant WL Purity DeltaEv CRI FC Cas Ev Lux 7 1568E 00 0 4066 y 0 4178 Ev Lux 7 1568E 00 0 2259 v 0 3482 CCT 3676 98 Dominant WL 576 30 Purity 0 47 DeltaEv 100 0000 CRI 77 14 FC 7744 Cas 78 62 6 196 6 368 D 2 676 Zoom Color Chart C C Lab Bar Radiometric Data Color Data gt 7 1568E 00 0 4066 0 4178 7 1568E 00 0 2259 0 3482 3676 98 576 30 0 47 100 0000 77 14 77 04 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 54 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc I ok Your Photonics Partner BWSpec 4 e CIE Lab space Spectrum Color Chart e Color Bar Spectrum Color Chart Color Chart Absolute Irradiance uW cm 2 nm
10. mW or cm nm Alternatively a spectral irradiance calibration can be used to calibrate the spectrometer for emissive photometric measurements such as measurements of the color of an emissive source for example LED Your setup conditions distance from source and lamp current must be as close as possible to what your NIST Certificate states in order to conduct a successful calibration process Ideally the light source should slightly under fill the spectrometer s angular aperture The light source illuminates the spectrometer at a distance specified in the lamp source s calibration data Given the correct experimental setup the spectral irradiance calibration proceeds as follows with the assumption that the spectrometer is equipped with a suitable input optic and linearity correction e Adjust the integration time so that the maximum spectral response feature is just below the spectrometer saturation level against the certified lamp at a specified distance Stop the acquisition and freeze all physical setup with minimal disturbance 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 45 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 ECUNIC List rarer a Measure Spectra SP 0 Spectrum Color Chart ZAOL Dark Subtracted 70000 60000 _ Spe
11. B amp W Tek Inc ERA FH Your Photonics Partner BWSpec 4 Click Smoothing to open the Smoothing option dialog box Refer to section 6 1 for more details Click Background Remove to remove the fluorescence background using an automated algorithm for Raman spectral analysis Click Derivative to apply derivative operation to an active spectrum Click Peak Analysis to open the Peak Analysis dialog box Refer to 6 2 for more details Click BAC151A Camera to enable the video camera support for BAC151A accessory for Raman analysis option only Click Export Data to export collected spectrum into txt file Click Timeline for programmed spectral data recording Click Wavelength Calibration to enter wavelength calibration functions Option Option Plug in Setup Help Language Enable Disable Linearity Correction Linearity Correction Relative Intensity Correction Enable Disable Relative Intensity Correction Reference Material Correction Enable Disable Reference Material Correction Irradiance Correction Enable Disable Irradiance Correction 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 11 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERA FH Your Photonics Partner BWSpec 4 Click Enable Disable Linearity Correction to enable or disable linearity correction function A uni
12. BWSpec Data ZIP File 5 e Save Selected Spectrum This is for saving the selected spectrum to a folder as specified Data Format TXT file txt 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 6 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc ERA FH Your Photonics Partner BWSpec 4 CSV file csv TXT file even SPC file spc BWTek Raman data file format BwRam Save All Spectra Into A Zip File Use this option to save all spectra to a zip file Data Format BWSpec Data ZIP File BwRamS oave all Spectra Automatically Menu option for saving all spectra into a specified file folder at one time with each spectrum being saved as a single file inside this folder Save all Spectra Manually Menu option for saving all spectra into a specified file folder each spectrum is created as a single file inside this folder The files are named in sequence as SP O txt SP 1 txt SP_4 txt etc But you can also change the file names manually Print Preview To view the current spectrum on screen as it would appear on the print out Exit To exit BWSpec 4 A message will be shown if there is a spectrum that has not been saved before exiting the software View 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 7 2900
13. Savitzky Golay Savitzky Golay Filters smoothing can be applied to the original spectrum with a window size setting between 2 5 This is a very commonly used smoothing method with less chance to cause distortion and degradation to the spectral resolution e Boxcar smoothing can be applied to the original spectrum using a window size setting in 1 1023 The smoothing function will apply the forward moving 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 23 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 smoothing by taking the averaging of the specified number of pixels plus one pixel on the left to the group and one pixel to the right of the group This is an effective smoothing operation at the expense of degraded spectral resolution hence do not use a larger than necessary window size setting in order to preserve the needed spectral info FWHM is a useful tool for analyzing spectral features When applied to the acquired narrow emission peaks such as peaks from low pressure mercury and argon lamps the FWHM value can provide practical measure of the particular spectrometer spectral resolution 6 2 Peak Analysis 6 2 1 Automatic Peak Identification From the menu bar click Tools Peak Analysis The Peak Analysis window will appear In the top left hand corner of the menu input the va
14. Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 38 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc FH Your Photonics Partner BWSpec 4 You must have a Probe connected to a Raman system or a Laser and a spectrometer in order to get proper results Make sure that the wavelength of the excitation laser you are using is entered into the Raman Excitation Laser Wavelength page on the Display Scales for example 85nm ud Display Scales Setting b a X axis Y axis Excitation Laser WaveLenath Upper Chart Raman Excitation Laser Wavelength 785 15 nm Lower Chart Determine the appropriate integration time use the standard reference material SRM2241 for 785nm or SRM2242 for 532nm to run a scan The integration time of the scan should be adjusted to the value so that the highest intensity level is just below saturation level 65000 counts Run a dark scan with the same integration time For B amp W Tek Raman system the laser turns off automatically when dark scan is collected For modular Raman spectrometer make sure the laser is off when a dark scan is collected Run a reference scan using SRM2241 or SRM2242 with the same integration time with laser being turned on 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact
15. Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 28 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc By ok Your Photonics Partner BWSpec 4 Select the path you would like to save these files into by clicking Save path then click Save to finish 7 4 From menu bar click File Open Spectrum m to open a previously saved spectrum into the graph area Choose the file type and file you want to open and click Open to finish 8 Read Flash Import Spectrum From the menu bar click Setup Flash Access a dialog box will be displayed for the currently connected spectrometer info and settings Click Read Flash at the bottom to restore parameter settings integration time multiplier etc to the BWSpec 4 default setting as saved in the flash Flash Access 15a3 2ec8 Manufacture Date 02 2010 Model ECONIC C_Code Hardware Code 112 50 SysFile Version 4 4 2 0 BwSpec Version 3 26_25 Number Of Pixel 2048 10 x Firmware Number DII Version CCD Type Mininum Integrate Time Timing Mode 1 Input Mode Pixel Mode 0 500kHz Start Pixel 71 End Pixel 1962 Coefficient A U 318 313125985675 Coefficient_A 1 0 443356105484327 Coefficient_A 2 3 443941 007402756 05 Coefficient A 3 7 051138591854E 10 Coefficient_
16. path where the ratio file will be stored Click Create button The Relative Intensity Correction file is created and the Ratio3 file is saved in the designated path Apply Relative Intensity Correction To turn on relative intensity correction feature from the menu bar Option Enable Disable Relative Intensity Correction Check the box Enable Relative Intensity Correction and click on to locate the Ratio3 file specific to the system calibrated with Click Set to apply o Enable Relative Intensity Correction Enable Relative Intensity Correction Relative Intensity Comection Ratio File Ratio3 txt C Program Files BWTEK BW Spec4ratio Ratio3 NYB tt sc e m Pixel Range End Pixel Start Pixel El Public C Code Coefs File Name Int Time Order Pixel Number Ratio Standard Wave Number Range End Wave Number Start Wave Numberinm 1945 136 NYB Double C Program Files BWTEK BW Spec4 vatio Vl 5000 5 2048 Double SRM 2241 R1 Raman 32 175 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 290020018 G BETA 7 12 12 2011 4 Copyright O 2011 B amp W Tek Inc ERA FH Your Photonics Partner BWSpec 4 BWS415 785S PRB Dark Subtracted 60000 50000 40000 30000 Relative Intensity 20000 10000 500 1000 1500 2000 2500 3000 Raman Shift cm 1
17. 0 750 800 Undo All Zoom Pan Wavelength nm 2 Online Darked No Dark Compensate No Liner Response Correction No 5 4 Restore the Initial View Place cursor in the graph area and right click your mouse and select Undo All Zoom Pan to go back to original spectrum view You may also use the double click of the left mouse for the same purpose ZCVO Raw Data Relative Intensity Save Image As 0 Show Point Values 700 750 800 Un Zoom Wavelength nm Undo All Zoom Pan N 2 Online Darked Dark Compensate No _ Liner Response Correction No 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 23 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 5 5 Copy and Save the Spectrum as an image Place cursor in the graph area right click to select Copy or Save Image As BRC112 FIT11 ZCVO Raw Data 50000 4 34 oer ect e err mem membr gt 40000 LIS Bele Sie Bolan oO c 2 2 309000 I ate ay nn q n e fi M 10000 B ss xls visis esos ste E vor asl uo veo n c py e v 1 Copy 4 Show Point Values 400 500 600 700 800 SLE Wavelength
18. 20018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ayy Wwe Your Photonics Partner BWSpec 4 Acquire Tools Option Plug ir Se Show In Overlay A Show In Sequence Spectrum List Panel Spectrum Info Panel Spectrometer Control Panel e Show In Overlay To display spectra in one graph as overlays e Show In Sequence To display one spectrum in a graph with a sequence of graphs e Spectrum List Panel This option is for activating a list panel This panel after being enabled lists sequentially all captured spectra A spectrum becomes active after it is selected from the list Otherwise an active spectrum is the last one acquired e Spectrum Info Panel This option is for activating a spectrometer info panel This panel displays associated operation setting parameters such as integration time used and average time used to the active spectrum e Spectrometer Control Panel This menu option is for a control panel to be displayed for configuring the graph display options Refer to 3 4 for details 3 1 3 Acquire 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 8 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 Tools Option Plug in Setup Acquire One Spectrum Acquire Continuously Acquire Dark Acquire Reference Acquire Overlay Shop Clear Display Overlay Reset
19. 830 Email www bwtek com contact 20 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 When more than one spectrum is displayed each one will have a different color Initially default colors will be assigned automatically to each spectrum on the spectrum display panel The colors can be manually changed to designed ones by following the below procedure On the menu bar click Setup Custom Setting Spectra Color Setting CHE L5 Spectra Color Setting Spectrum H1 H3 Spectrum 11 2 19 393 20 oil Relative Intensity ENEEENH NH Custom eclars 4 8 E E d N Custom Colors gt gt Wavelencth nin Cancel Dark Compensate Liner Res 2 Cnline Darkec Click on the of the spectrum you d like to change choose color click OK and Apply Click Set to save the setting or Close to close the window without saving the setting 4 5 Spectral Graph Setting opectral Graph Setting is for configuring the graph background color spectrum display line width and font size in the graph area On the menu bar click Setup Custom Setting Spectra Graph Setting 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 21 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W T
20. 830 Email www bwtek com contact 32 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 oJ Derivative Apply Derivative Derivative Type Point Diff Savitzky Golay Differential 9 4 Timeline Timeline function provides automated timed recording of spectra according to the scheduled time interval settings with acquired spectrum at the time specified and saved into individual files 9 4 1 Timeline Equal Integration Time Click Tools Timeline Timeline Equal Integration Time a dialogue box will appear Before starting timeline scan you need to enter the settings in this box including interval time number of scans file name prefix file name postfix and save path Click 9 button to start timeline scan The spectra collected will be saved in the designated path If no specific suffix is assigned default 1 2 will be assigned to each spectrum 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 33 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc IP Your Photonics Partner BWSpec 4 File View Acquire Tools Option Plug in Language Heb Timeline Equal Integration Time Save Spectrum Acqure spectrum inlerval time 100 21 File Name Prefix _ Declaration Information Title Exanple Pisel iw avelengthiw avenumber Raw Da Total Acq
21. B 0 721 411587371491 Coefficient_B 1 2 27398272298524 Coefficient_B 2 0 0001 75401063497116 Coefficient_B 3 421085235831 429E 07 TE Cool No Slit Size 25 Grating Lines And Blaze Waveleng 600 400 Filter Type LVF Cylindrical Lens No Batch Number Patch Number Security Code Application Code Number Of Calibration Points 13 Pixel 106 Wavelength 3650 15 2 Flash Access Successfully 9 Plug in From the menu bar click Plug In Plug in Manager to enable or disable functions by selecting or deselecting function modules Click OK to finish 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 20 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERI ok Your Photonics Partner BWSpec 4 Plug in Management Name Assembly 00 0 0 0 0 0 0 0 0 O C Remove Background Plug in BwRam Plugln BackgroundRemove dll Remove Background J BAC 151 Camera Plug in BwR am Plugln CameraBACT Ex dll Open 151 Camera by this plug in BWIQ Plug in BwR am Plualn Connect2B wl dll BWIQ can acquire spectrum directly thre Esport Data Plug in am Plugln E portD ata dll Export all spectra data into one text file 4 C Linearity Correction Plug in New method BwR am Plugln LinearityCorrection2 dll Linearity Correction Plug in new methoc Spectra Math Plug in BwR am Plugln SpectraM ath dll D
22. Data a dialog box will show up Select the data information you want to export click Save as button All spectra data will be exported and saved into a text file 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 3l 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc IER ok Your Photonics Partner BWSpec 4 Export All Spectra Data to One Text Fil Ioj x Title Head line Pixel Sample 1 Sample 2 Spectral Units Pixel wavenumber cm 1 Wavelength nm Spectral Data Raw Data P AllspectraData 2011 10 31 11 38 15 txt Notepad Dark Absorbance File Edit Format View Help Reference Absolute radiance Pixel 400F HG 365 parksub 36 1663 00 Dark Subtracted 38 1735 00 Data Spacing C Tab C semicolan comma 43 1789 00 C semicolon Tab C comma Tab 46 1903 00 49 1710 00 9 3 Derivative Click Tools Derivative next click a dialogue box will appear for spectral derivative function options Three derivative methods are provided for possible data pretreatment use e Point e Savitzky Golay e Differential Select the desired derivative method check Apply Derivative box to apply the derivative calculation to the currently active spectrum 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7
23. Material Correction e Click E to locate the Reference Material correction ratio2 txt file e Click Set 9 8 Absolute Irradiance 9 8 1 About Irradiance Calibration Irradiance Calibration can be conducted by BWTek Inc using NIST Traceable Standard Irradiance Standards and calibration setups if so desired Irradiance Calibration is an option that can be ordered at the time of placing a spectrometer purchase Before activating your Irradiance Calibrated unit using the accompanied calibration file Linearity Correction should be performed first 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 44 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 9 8 2 How to Conduct Irradiance Calibration This section is for advanced users only In order to conduct Irradiance Calibration you must first have a NIST Traceable Lamp source with Certified Values which cover the spectral range you will be calibrating your spectrometer to and DAT file that will have accompanied your lamp Another factor is having the correct input optics for your spectrometer to collect the light such as a fiber and Collimating Lens as an example The spectral irradiance calibration will allow a spectrometer to convert spectral measurements to absolute spectral irradiance measurements in units of light power in W
24. Step Input Distance between the lamp and the spectrometer 50 Step 7 Input Transmittance of Neutral Density Filters 100 Neutral Density Filters NO Density Filter Step 8 Set ratio name Ratiol_ZAOL Set ratio file path E Program Files BW TEEME S pect A E Step 9 Create ratio Create e Step 3 Input the distance for your NIST Calibrated Light Source at which it was calibrated This information will be contained on your Certificate or the DAT file e Step 4 Coefficient Order used for Curve Fit default value 7 e Step 5 Select which spectrum to use for your Reference Data If you ve already taken a Reference Scan select Use Current Reference Data e Step 6 Select the distance that you have setup between your input optic and your certified light source Also make sure to enter into the dialog box all calibration related information such as the additional optics used etc Be careful to select correct units to match the certified data provided as in the reference certified lamp data file 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 47 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 e Step 7 Input transmittance of neutral density filters e Step 8 Name the ratio File in which you would like it to be saved as We sugg
25. ak you want to add hold down the left button of the mouse and drag a dotted line around the peak The boxed peak will be added to the list after the mouse button is releases aaa Display Peak List Add or modify a peak manuall Peak Analysis 5 Absolute 3000 ES Find Display Peak In Graph i Height Display Peak X Value Display Peak Area Fill Clear All Display Peak Y Value Display Peak Start and End Maximum Start Maximum End Start Ram Peak Index Start Pixel Pixel End Pixel W avelength nm w avelength nm Wavelength nm Shift cm 1 353 25 364 76 367 26 27 528 41 ll eame emm Relative Intensity 727 30 5385 0 team t uei esee 22 740 750 Wavelength nm Modify a Peak Manually Check the box for Add or modify a peak manually hold down left button of mouse to drag a dotted line box around the spectral feature that needs to be modified until you are satisfied with the change Release the mouse for the modification result to be displayed Report Point cursor to a Peak Index and activate this line right click you mouse and select Export Result at popup menu you can view a print preview of the report 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 27 290020018 G BETA 7 12 12 2011 Copyrigh
26. ance 10 Photometric Conversion Stat WL app Photopic EndWL pg Interval WL jo Set Cancel Apply 9 9 2 Reference and Dark Scan for Measurement Scotopic To perform a 9o T R based color measurement a white reflectance standard in reflectance setup and a stable light source covering the color range should be used Light sources such as the BPS101 BPS120 from BWTek are suitable candidates As the apparent color of reflective objects depends upon both the spectral response of the spectrometer and the spectral output of the illumination source a reflectance reference scan should be performed often so that the error due to the lamp drift is minimized Spectrometer BPS101 Light Source Bifurcated Fiber Bundie Plastic Sample Material 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 51 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc BW I Your Photonics Partner BWSpec 4 Connect the Light Source and the spectrometer via a fiber reflectance probe Turn on the light source and allow it to warm up e Perform a Reference Scan e Dark Scan using the same integration as the reference scan Make sure to place the probe onto an absorbing black material or physically block the light from entering into the probe for dark scan 9 9 3 Irradiance for Color Measureme
27. anual Timelines Timeline data_9 txt 9 5 Linearity Correction 9 5 1 About Linearity Correction Linearity correction will adjust the detector array s non linear response to follow a calculated linear relationship against received detector photons using the calibration function obtained through a linearity calibration correction For example a typical detector array exhibits nonlinear response with respect to incident photons especially at low light level as well as close to the saturations In these regions doubling photons or doubling detector exposure time will no longer result in two times the detector s output deviating from a desired linear response range NOTE The Linearity Correction must be conducted by the factory in order for the calibration information to be saved into device memory a copy will be recorded at the factory Linearity Correction is an upgrade option item to be ordered at the same time of the spectrometer purchase 9 5 2 How to Conduct a Linearity Correction For advanced users the linearity correction may be conducted at the customer site From the menu bar Option Linearity Correction The linearity correction window will appear 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 36 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 M Linearity Correction Int
28. ck button to manually load the spectrum file backwards one time e Click button to resize the Timeline Load window 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 35 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc I ok Your Photonics Partner BWSpec 4 2515 Load path C Documents and Settings Dawn My Documents BWSpec4 U ser Manual Timeline Auto Load a spectrum every ms 200 H eo Mai C Documents and SettingssD avin Mvy DocumentssBw Spec4 L ser M anual Timeline Timeline data_1 txt C Documents and Settings Dawn My DocumentssBw Spec4 Ll ser M anual Timeline T imeline data 10 151 C Documents and Settings Dawn My Documents BWSpec4 User Manual Timelines Timeline 2 4151 C Documents and SettingssD awn My Documents BWSpec4 User Manual Timelines Timeline data_3 txt C Documents and Settings Dawn My Documents BWSpec4 User Manual Timeline Timeline data_4 txt C Documents and Settings Dawn My Documents BWS pec4 User Manual Timeline T imeline data_5 txt C Documents and Settings Dawn My Documents BWSpec4 User Manual Timelines Timeline data_6 txt C Documents and Settings Dawn My Documents BWSpec4 User Manual Timelines Timeline 7 t t C Documents and Settings Dawn My Documents BWSpec4 User Manual Timelines Timeline 8 15 C Documents and Settings Dawn My Documents BWSpec4 User M
29. ctrometer Control Panel nx 50000 Wavelength nm Wavenumber cm 1 C Raman Shift cm 1 C Wavelength linear nm Y Axes Type C Raw Data C Dark Reference Dark Subtracted Transmission Reflection Absorbance Absolute 40000 Relative Intensity 30000 20000 10000 Extemal Trigger 400 500 600 700 800 900 1000 Wavelength nm Spectrometer 42 Spectrum Infor Creation of Irradiance Ratio File From the Menu Bar select Option Irradiance Correction Follow the steps stated in the following window to proceed with irradiance calibration procedure Create Irradiance Correction Ratio File BE xl Step 1 Turn aff light take a dark scan then turn an light take single raw data scan Step 2 Please Select the Location of the Lamp File Lamp Data Info Start wL W avelenath nm Absolute Irradiance End WL Inc WL Step 3 Input Distance and unit of Lamp Data Distance 50 2 nm Shep 4 Select Curve Fit Method v Regressions C Interpalatians Order 7 Step 5 Select D ata Use Current Spectrum Step Input Distance between the lamp and the spectrometer 50 cm Step Input Transmittance of Neutral Density Filters 100 x Neutral Density Filters Netral Density Filter Step 8 Set ratio name Set ratio path C Program Files B TEE ABS atio Step 9 Create ratio
30. e Setup L a b 0 0 0 Wavelength for Color Calculation Radiant Unit 4 T R Distance ij em Start WL 400 nm End WL Fo Interval WL 10 nm th for Color Calculation box ub Color Measure Setup mius Reference Color Setting Observer 2 degree Wavelength for Color Calculation Color Calculation Source Color Calculation Source TAR Distance Start WL End WL e Select Distance Start WL End WL and Interval WL for the measurement e Select color measurement standard from Color Standard box including Illuminant and Observer 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 50 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 Color Measure Setup gt e ud Color Measure Setup Reference Color Setting Color Standard R R R Reference Color Setting Color Standard Illuminant Wavelength for Color Calculation Radiant Unit mW nm Color Calculation Source TAR Distance em Start WL MO End WL 70 nm interval WL ij Wavelength for Color Calculation Radiant Unit mW nm Color Calculation Source Dist
31. egration Time Setting Start Integration Time ms 1 Step Integration Time mz 1 End Integration Time ms 40 Intensity Range Setting Nose Value 2000 Saturated value 6 3000 Polunomial Fit Polynornial Fit Order 7th order Pixel 200 400 BOD 800 1000 1200 gt Eee Hl e Set the parameters for linearity correction on the screen e Press to start collecting the scans needed for linearity correction e Press to collect a dark scan with light off Press 27 to calculate linearity correction coefficients The Linearity Coefficients will be displayed on the top of the window along with a message 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 37 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 The linearity correction coefficients have been saved to para ini file YAXIS CORRECTION section NOTE Make a backup copy of the parameter file with the new Linearity Coefficients that have been written to them for future reference Once the Flash is reread the Linearity Coefficients will be lost If you save a copy of the parameter file with the Linearity Coefficients already saved on it you may Import this parameter file into the BWSpec software when needed NOTE The key for a successful linearity correction calibration s
32. ek Inc Your Photonics Partner BWSpec 4 A dialog box will show where spectral graph settings can be adjusted a Graph Setting r Spectra Mame Fil Background Background Color r Graph Background Color Chart Background Color Graph Background Color r Grid Display Vertical Grid Display Horizontal Grid Display Zero Line Font Bold Font Size 12 AntiAlias Line Width 5 Spectrum View 5 1 Zoom In To zoom in a spectrum hold down the left mouse button and drag the cursor to make a box around the area you wish to zoom in on 5 2 Pan Hold Ctrl key and hold down left mouse button move the cursor in the direction of the area you wish to view 5 3 Restore of View Place cursor in the graph area right click your mouse and select Un Zoom to zoom out 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 22 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 BRC112 FIT11 ZCVO Raw Data Relative Intensity Copy Save Image As Show Point Values 0 a 70
33. en irrands_DispWLMin 350 Display Scales Settings irrands_DispWLMax 780 Display Scales Settings start wv_irrands 350 Start WL for Irradiance Calibration should match Lamp StartWL 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 59 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Lamp Unit Index 2 ERA FH Your Photonics Partner BWSpec 4 end_wv_irrands 780 End WL for Irradiance Calibration should match Lamp End WL interval_ wv_irrands 10 Increments of WL pts from Start WL to End WL should match Lamp Pixel value Ratio w out Linearity Correction Ratio with Linearity Pixel Ratio Correction LL HMM NN 276 6 89485036671211 5 5 70395476167662 5 LL Hd MM Hd MM 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 60 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc ERA FH Your Photonics Partner BWSpec 4 Appendix B Definition of Irradiance Data File Format Example of a Certified Reference Lamp data file 1e D300 DAT Tungsten lamp Light Touch Software Data File 2 3a 0 TUNGSTEN 16V 6A 2020 31B W Supp Lens NM 7DH 290 1110nm DC 51C 200 1010nm Silicon 200 1100nm 06 13 2003 14 38 16 RS 10A S N HL0191 IRRADIANCE Se eee eee start flag 350
34. est your keep the default naming scheme Ratio1 C code of the spectrometer Save Path Location to where the ratio file will be saved to e Step 8 Click Create to generate the Irradiance Ratio File 9 8 3 Apply Irradiance Ratio File e Select Option Enable Disable Irradiance Correction e Check on box Enable Irradiance Correction e Click to locate the irradiance correction Ratio1 txt file Click Set e Click Absolute Irradiance on the Spectrometer Control Panel The Absolute Irradiance curve will show on the display Spectrometer Control Panel n x Enable Cursor Display Options Axes Unit Pixel Wavelength nm wavenumber cm 1 C Raman Shift cm 1 wavelength linear nm Y Anes Haw Data Dark Reference Dark Subtracted Transmission A election Absorbance Absolute irradiance ce eee ee Acquire Control Extemal Trigger Spectrometer m Spectrum Infor You may need to adjust the X axis and Y axis scales in order to get the suitable display scale settings 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 48 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc I ok Your Photonics Partner BWSpec 4 Yew Plugin Setup Help Language LA mm 9 2 22 0 Shi
35. etup is to provide an adjustable intensity light input into the spectrometer to be calibrated so that the spectrometer shows a light response 500 1000 counts above its dark levels at its shortest integration time This will provide the calibration with maximum useable calibration dynamic ranges 9 5 3 Activate Linearity Correction To activate linearity correction from the menu bar Option Enable Disable Linearity Correction 9 6 Relative Intensity Correction 9 6 1 About Relative Intensity Correction Relative intensity correction is specific for Raman spectrometer users Its purpose is to correct individual spectrometer s unique relative spectral responses against a traceable standard The corrected spectral response of a Raman spectrometer makes it possible for meaningful comparisons between data obtained from different instruments The calibrated spectral response also reveals quantitative vibrational intensity information between different Raman features within a given Raman spectrum The relative intensity correction involves the use of Standard Reference Material 2241 SRM 2241 for Raman spectrometers where a 785 excitation laser source is used and SRM2242 where a 532nm excitation laser source is used 9 6 2 How to conduct a relative intensity correction This calibration is an option a customer may order at the time of purchase For advanced users this may be conducted at a customer site with appropriate equipment 19
36. file Create 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 46 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc BW I Your Photonics Partner BWSpec 4 e Step 1 Turn off light take a dark scan Then turn on light take a single raw data scan e Step 2 Click EN button and locate the lamp data file for the light source you are using for irradiance calibration Your lamp data file must be configured in a manner in which BWSpec can read it Please see the Appendix at the end of this manual section called Definition of Irradiance Data File Format for information on formatting You can also view the file D301 dat for an example of format which can be located in the folder where BWSpec is installed Create Irradiance Correction Ratio File nm x Step 1 Tum off light take dark scan then turn en light take single raw data scan Step 2 Please Select the Location of the Lamp File C Program Files B w TEKABWSpecd ratio Files Inadiance Source Lamp Data DZA33 0621 07 VIS FIb _ Lamp Data Info Start wL 300 wavelength nm Absolute Irradiance End WL 1100 0 01149004 Inc WL 0 07336169 Step X Input Distance and unit of Lamp Data Distance 25 Unit uvicm 2 nm Shep 4 Select Curve Fit Method Regressions C Interpolations Order 7 Step 5 Select Data Use Current Spectrum
37. i epa eoo von ex pod 19 4 4 Spectrum Color 22222222052 20 45 Special Grabh Seling occ E 21 ME rug Bd P 22 Di ZOOM 22 52 22 92 Of VICW c 22 5 4 Restore the Initial Iis 23 5 5 and Save the Spectrum as an image 24 SNOW VAS E E T 24 6 Spectral Processing essssssssssesseeeccossssssssseccccossssseessseeccsessssessssseeeos 25 6 1 Spectral Smoothing SERRE TES REO 25 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 2 290020018 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 26 7 Save and Import Spectrum 28 71 Save A SINGICS SPCC tH sacssscssssicassacessscecsasasscassandaaaaassasseaessnasasesasacasessscaaaaaeasseassevecaionass 28 7 2 Save All Spectra Into 28 7 3 Save All spectra To One File 4 eere eene 28 re IMPON SCC TS 29 TRO AC FAS mU T m 29 MEM UL 29 9 1 Backgrou
38. iance Data InadianceDistar 0 IradiancelntTin O radiance Ratio Iradiancelnitlr 0 LaserLevel 0 Laser Wavelen 0 Model BRC112 FIT11 Name SP_1 Save Path Time Multiply 1 Total Int Time 1 WS Spectrometer Spectra Inform 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 17 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 3 6 Status bar 1622 Onine DarkedNo Dark Compensate No Liner Response Comecton No Stop acquired 4 Spectrum Display A spectrum can be displayed in two modes e Show In Overlay e Show In Sequence 4 1 Show In Overlay Mode Click View on the menu bar and select Show In Overlay SBR Measure Spectra Raw Data amp v SP_O T SP 2 Spectrometer Control Panle nx 2 o E ts o 700 Wavelength nm Spectrometer 4 2 Show In Sequence Mode Click View on the menu bar and select Show In Sequence 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 18 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc RC112 FIT11 Spectra List Panel ax SP_O 8 1 5 1 SPT 4 Spectrometer m Spectra Inform 4 3 Spectr
39. ics Partner BWSpec 4 e Click Color Setup for color measurement function settings ioli Reference Color Setting Color Standard L a b Illuminant Des E 0 0 2deqree Observer degree Wavelength for Color Calculation Color Calculation Source Radiant Unit m nm Color Calculation Source Distance Start WL ion nm End wL 700 nm Interval VL nmn Set Cancel Apply 3 1 8 Help e Click Help About to find out the current version of the software Click Help Help PDF to display the User Manual of the BWSpec 4 software Language About Help POF 3 1 9 Language Click Language to select a display language desired for the software English or Chinese wv English Chinese 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 14 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 3 2 Tool bars Open spectrum Save spectrum This bar is for operation parameter setting 1 400 al E including current integration time integration time multiplier and time average Current Integration Time Integration Time Multiplier Current Integration Time Integration Time These buttons for spectrum acquisitio
40. lue at Minimum Absolute Peak Height threshold and click Find to identify all peaks that exceed the threshold All identified peaks are listed in a table in the window This table provides you detailed information on the identified peaks Check the box in front of Display Peak In Graph and the identified peaks will be marked on the spectrum 6 2 2 Delete an Identified Peak Right click Peak Index for the peak you want to remove from the list and select Delete This Peak and the removal will be done Peak Analysis PEE Minimum Absolute 3000 z Display Peak In Graph iv Display Peak List Peak Height Display Peak X Value Display Peak Area Fill SP D Clear Iw Display Peak Y Value W Display Peak Start and End fades Start Pixel Heel Eme ER Stark hd asimum End Start Ramar Wavelength nm Wavelength am Vavelength nm hift em 1 pem 27 528 41 Delete This Peak AF 404 38 406 34 25 996 32 Export Result 407 81 410 26 24 609 74 414 424 435 03 433 85 23 140 59 649 545 06 550 04 18 438 88 713 T22 576 50 577 04 17 958 62 4 k 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 26 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 6 2 3 Add a Peak Manually 6 2 4 6 2 5 Check the box for Add or modify a peak manually Place cursor on the pe
41. n Refer to 3 1 3 for details Print spectrum 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 15 Copyright 2011 B amp W Tek Inc 290020018 G BETA 7 12 12 2011 Bay we Your Photonics Partner BWSpec 4 Reset the scale Delete all current imported and acquired spectra Set the X amp Y axis unit and scale 3 3 Spectrum List Panel Spectrum List Panel is located on the top right side of the main screen after being activated Spectra List Panel E Measure Spectra SP 0 SP 1 SP2 The spectra with V signs indicate that the spectra are selected for being displayed the current graph while the spectra without the signs are hidden from the current graph display The spectrum with indicates the currently active spectrum selected 3 4 Spectrometer Control Panel 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 16 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 Change the X axis units nd the Y Axis type WE Spectrometer 88 Spectra Inform 3 5 Spectrometer Info Panel Information on the spectrometer and Dark Sub False n Data Saved False spectrum you are Derivative Data currently working with Int Time 1 Irad
42. nd Removal univoce 30 9 2 5 31 9 3 32 PME eae E EE A E 33 9 5 Linearity 36 9 6 Relative Intensity 38 9 7 Reference Materials Correction 42 9 8 Absolute IPFACIANCS ee 44 9 9 Color 49 Appendix Irradiance Ratio File Data Definition 59 Appendix B Definition of Irradiance Data File Format 61 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 3 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ERA Your Photonics Partner BWSpec 4 1 Introduction BWSpec is a spectrometer data acquisition operating software program used for B amp W Tek spectrometers 2 Installation 2 1 2 2 Operating Systems Supported e 32 bit Windows Operating Systems including Windows 98 98 Second Edition Windows ME Windows NT 4 Windows 2000 XP Windows Vista Windows 7 e 64 bit Windows Operating Systems including Windows XP Windows 7 oelect an a
43. nm Undo All Zoom Par 5 6 Show Values Place cursor in the spectrum display area right click to select Show Values from the popup menu Values associated with the current data point of the spectrum in pixel wavenumber and wavelength will be displayed e c 2 5 Save Image As Show Point Values N Indo All Zoom Pan 600 700 800 Wavelength nm 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 24 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ayy Your Photonics Partner BWSpec 4 6 Spectral Processing 6 1 Spectral Smoothing 101 Apply Smoothing After Scan Derk Subtracted smoothing FFT Fast Fourier Transform FFT Smoothing Degree 1 99 10 savitzrky Golay Smoothing Sav Golay Window Size 2 wt1 2 5 E Boxcar Smoothing Boxcar Window Size 2 1 1 102337 Operate all spectra include unchecked spectral Indo The Spectral Smoothing dialog box can be activated from menu bar Tools Three smoothing methods are provided in BWSpec4 for effective S N enhancement e FFT Fast Fourier Transform smoothing can be applied to the original spectrum using a smoothing factor of in 1 9996 The smoothing factor needs to be carefully selected so that not to cause distorted spectrum e
44. nt To perform irradiance calibration based color measurement e From the menu bar select Setup Color Setup A dialog box will display where the settings for color measurement can be adjusted Inside the box for Color Calculation Source select Irradiance for the measurement ad Color Measure Setup c Reference Color Setting Color Standard llluminant D65 Observer Wavelength for Color Calculation Color Calculation Source Radiant Unit mW nm Color Calculation Source Distance 10 cm Photometric Conversion Start WL 4 i 400 hm Photopic End WL 700 Interval WL Scotopic e Enable irradiance calibration from Option Enable Disable Irradiance Correction and select the corresponding irradiance correction ratio file Ratio 1 txt e Calculate color perceived with the Photopic or 9 9 4 Color Analysis 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 52 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc BW IH ok Your Photonics Partner BWSpec 4 To acquire sample color data e Place the probe onto the desired sample to be measured e Acquire data scan on the sample material intended for color analysis ZCSW Dark Subtracted ES o c g o 2 700 Wavelength 9 9 5 Data Display Click the
45. o some Spectra Math with this plug in Timeline Plug in BwR am Plugln TimeLine dll Acquire spectrum and save it to spectru Wavelength Calibration Plug in BwR am Plugln wW aveLenghtCalibration dll Calculate the convert coefficient betwee lt gt OK Apply Cancel The selected Plug In functions will be available and shown under Tools and Option 9 1 Background Removal The Background Removal function is to remove spectrum background or to perform baseline correction Click Tools and select Background Removal A display window with three spectra will be displayed raw spectrum automatically fitted background curve and the spectrum after background removal Lambda factor can be adjusted for manipulating the auto fitted background so as to achieve the desired background removal result Click Do to apply changes 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 30 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc By ok Your Photonics Partner BWSpec 4 a Do Di Lambda 12000 226115 _ D IV Display Dynamic Operate all spectra Inclued unchecked spectra BWS415 532 Dark Subtracted Raw spectrum wo c 2 Raman Shift cm 1 9 2 Export Data Click Tools from the menu Choose Export Data Export All Spectra
46. pplicable installation package before proceeding with the installations Installation Insert the BWSpec4 CD into the disc tray An installation screen will appear for installation procedures Follow the installation instructions through the install wizard After BWSpec4 00 is installed successfully a shortcut icon will be created automatically on the desktop 3 Software Main Screen opectrometer Models with USB Interfaces Connect the spectrometer to USB Port of computer via USB Cable Launch BWSpec exe by clicking the shortcut icon on the Desktop From the BWSpec4 00 Menu bar Tools Flash Access Click Read Flash and the unit specific parameters for the unit will be read from the spectrometer to the BWSpec settings through the USB connection 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 4 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc Your Photonics Partner BWSpec 4 e After the Read Flash Completed message appears click Close button on the bottom right e The spectrometer is now successfully set up to the connected computer for operation ixi Fie Acquire Tools Option Plug in Setup Help Language UU e HE NewForm 5 Open Spectra Spectrum Color Chart 4007 HG 546 400F HG 546 07 Dark Subtracted Spectrometer Control Panel MX Enable Curso
47. r Display Options X Axes Unit 4 C Pixel Wavelength nm Wavenumber 1 C Raman Shift cm 1 Wavelength linear nm Y Axes Type C Raw Data Dark C Reference Dark Subtracted C Transmission Reflection C Absorbance C Absolute Irradiance Relative Intensity Acquire Control Spectrometer Spectrum Infor BWSpec 4 Main User Interface Screen 3 1 Menu bar File View Acquire Tools Option Plug in Setup Help Language 3 1 1 File 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 5 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc ER Fi Your Photonics Partner BWSpec 4 View Acquire Tools Option Plug in i New Window Open Spectrum Save Selected Spectrum Save All Spectra 45 0 Manually 7 Save All Spectra Automatically Save All Spectra Into ZIP File Print Exit e Create New Window Click New Window to create a new window of graph display with applicable panel for use with all available functions This is a useful tool when loading a saved spectrum for reference or comparison purpose against acquired spectra in separate graphs e Open Spectrum Click Open Spectrum to load one or more spectral files into the graph display Data Format BWSpec Data File BwRam TXT File txt SPC File spc
48. rtner BWSpec 4 With gradient integration time the integration time will change automatically according to the setting When you finish one time acquisition the integration time will automatically plus a step time on your last integration time which will then be you next acquisition integration time You can input step time in the dialogue box below After each scan the integration time will plus this step time as your next acquisition integration time to be used Timeline Gradient Integration Time 21 14 Save Spectrum Start Integration Time Name Prefix if Declaration Information Tite Piel Wavelength Wavenumber Raw Data 1 El w Data tems hw Pixel Wavelength m Wavenumber Raman Shift Save path C Users Test Documents Data p Rererence Raw Data Dark Subtracted oe Absorbance Absolute Step Integration Time 1 Suffix End Integration Time 65535 File type 9 4 3 Timeline Load To automatically load the files that were saved previously click Tools and select Timeline Timeline load click load path to select the folder location of the timeline data while the corresponding files are displayed on the Spectrum display panel e Click 4 button to automatically load each and every data file one by one e Click button to manually load the spectrum file forward one at a time Cli
49. t 2011 B amp W Tek Inc BW Wwe Your Photonics Partner BWSpec 4 7 Save and Import Spectrum 7 1 Save A Single Spectrum From the menu bar click File Save A Single Spectrum or click B on the tool bar to save the spectrum A Save Spectrum window will show where user can select path name file type as well as the data format related choices Save Spectrum Data 101 x El Save Spectrum Save path sDawn My Documents BwtekD ata V Declaration Information PE P0 Title Pixel Wavelength wavenumber Raw Data 1 Data ltems File type Pixel v Wavelength Wavenum ber Raman Shift v Dark Rererence iw Raw Dat Dark Subtracted Absorbance Absolute Inadiance gt Here is another tip for you to save place cursor on the spectrum name that you wish to save in Spectra List Panel right click your mouse and select Save This Spectrum to save 7 2 Save All Spectra Into ZIP file From the menu bar click File Save All Spectra Into ZIP File to save all of the displayed spectra as a zip file The saved spectra will show up in the graph area once you open this file 7 3 Save All spectra To One File Folder From menu bar click File Save All Spectra To One File Folder to save all spectra into a specified file folder where each spectrum will be saved as a single file These files will be named as SP O txt SP 1 txt SP 4 txt etc 19 Shea
50. t 12 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc I ok Your Photonics Partner BWSpec 4 e Click Flash Access to access the spectrometer specific info associated with the currently connected spectrometer e Click Display Scales to configure the X Y settings and scales e Click Custom Setting for custom spectral settings including Spectra Color Setting Spectral Graph Setting as well as access to the spectrometer info for the currently connected spectrometer Setup Help Language Eo AX Flash Access 2 Display Scales ez Spectra Color Setting Hardware Setup A Spectra Graph Setting Color Setup Spectrometer Info e Click Hardware Setup for spectrometer common settings as well as AuxPort settings Hardware Setup Bl x Common Setting External Trigger Timeout 0 se 0 mz Close Shutter on Dark Scan Close Laser on DarkScan Close LED on Scan Montoring Spectrometer Temperature Montoring Laser Temperature Montoring 261 Detector Temperature Montoring 261 Sample Temperature Enable Camera m m mim mimi Enable Factory Access Close 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 13 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc I Your Photon
51. t specific correction file will need to be supplied in order for the correction to work correctly Click Enable Disable Relative Intensity Correction to enable or disable relative intensity correction function This is a function related to correcting the unit spectral response against a known reference A unit specific correction file will need to be supplied in order for the correction to function Click Enable Disable Reference Material Correction to enable or disable reference material correction function This function is useful for accurate measurements in applications such as percent reflectance A reference material with certified reference value file will be needed in order for the correction to work accurately Click Enable Disable Irradiance Correction to enable or disable irradiance correction function A unit specific correction file will need to be supplied in order for the correction to work correctly Plug in Click Plug in to open the Plug in Manager dialog box where all available plug ins are listed Check corresponding box to choose the plug in functions you need Click Apply and then OK The settings with checked plug ins will be saved automatically Setup Help Language p Flash Access Display Scales Custom Setting Hardware Setup lt Color Setup 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contac
52. tion Reference Material Collection e Click to browse to locate the Reference Material ratio txt file You will be directed to the default path where this file is stored at C Program Files BWTEK BWSpec4 ratio This file contains the certified values of spectral 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 43 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc I Your Photonics Partner BWSpec 4 reflectance over the interested wavelength range After the file is loaded the standard data info will be populated x Step 1 Please Select the Location of the Standard A atio File C Program Files Bw TEE Bw Specd ratio S H5 33 01 0 tet Standard Data Info WaveLenath nm Data Start wL 360 End WL 830 Inc WL 10 Step 2 Select Fit Method i ep select Lurve Fit Metho Regressions nterpolations Step 3 Select Coefficient Order E Step 4 Set ratio file name Ratio2 zADL Set ratio file path C Program Files Bw TEE SBwSpec4 R atia Step 5 Create ratio file Create e Follow the steps stated to fill out the parameters for the correction The default ratio file name is Ratio2 spectrometer code e Clock Create to generate Ratio2 file 9 7 3 Enable Reference Material Correction Files e Select Option Enable Reference Material Correction e Check on box Enable Reference
53. uire Specta Number 20 File Name Suffix Data Pixel Wavelength W avenumber Dark Raw Data Dark Subtracted Rererence ZTR bsorkance File type Save path C Documents and Settings chris My Documents BwtekD ata Status lt I lt I lt I lt I lt I I lt lt Relative Intensity Wavelength nm ud Spectrometer ug Spectra Infor Online Darked No Dark Corrpensate No Liner Response Correcton No 7 acquired Timeline Equal Integration Time Acquire spectrum interval time 100 File Name Prefix _ zb formation f le ex MW relent gi h la imber R ay Da Total Acquire Spectra Number 20 File Name Suffix 3 ltem 5 w File type wavelength vavenumber D L Raw Data Dark Subtracted eference Save path and Settinas chns My D ocuments Butekl don Status The data file save successed C Documents and Settings chris My Documents BwitekDataxdata 11 txt ZTR Absorbance 9 4 2 Timeline Gradient Integration Time Click Tools and select Timeline next click Timeline Gradient Integration Time a dialogue box will appear 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 34 290020018 G BETA 7 12 12 2011 Copyright O 2011 B amp W Tek Inc BW we Your Photonics Pa
54. um Display Setting On the menu bar click Setup Display Scales or simply click on the tool bar A dialog box will display where the scale settings on both X and Y axis can be adjusted Display Scales Setting Seles axis Y axis Raman E citation Laser w aveLength Scales Unit Upper Chart Automatic Manual Wavelength nm 353 56 Pixel Pixel Raman Shift cm 1 b asinum 013 10 Pixel Lett Position J Lower Chart Right Position Set Cancel Apply 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7830 Email www bwtek com contact 19 290020018 G BETA 7 12 12 2011 Copyright 2011 B amp W Tek Inc By ok Your Photonics Partner BWSpec 4 4 3 1 Unit for X axis Click on X axis tab you can set X axis to one of these three units e Wavelength nm e Pixel e Raman Shift 4 3 2 Unit for Y axis Click on Y axis tab you can set the Y Axis to e Automatic scale e Manual scale e Logarithmic e rradiance unit available when the function is enabled xi Hanis Raman Excitation Laser WaveLength Scales Unit Upper Chart Automatic Manual Minimum animum B5535 Lower Chart Sel Cancel Apply 4 4 Spectrum Color Setting 19 Shea Way Newark DE 19713 USA Web www bwtek com Tel 302 368 7824 Fax 302 368 7

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