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Jenway 6850 Spectrophotometer Manual

1. 00 abs UL ent Params option The user will be prompted to enter the _ constants that are required for the selected curve fit algorithm 1 Linear Fit KO K1 x Abs 2 Square Fit KO K1 x Abs K2 x Abs 3 Cubic KO K1 x Abs K2 x Abs K3 x Abs 4 Linear Fit Through Zero K1 x Abs The values can be entered using the numerical keypad Press the Enter key to confirm 6 3 2 3 Edit Calibration Table The user can edit the calibration curve data by pressing d 140 Unm Abs Setup Standard Conc the function key below the Standard option The user will be prompted to enter the concentration values for each standard The values can be entered using the numerical keypad Press the Enter key to confirm To add additional standard values to the table press the down arrow on the keypad Once all values have been entered press the Esc key on the keypad to return to the calibration table settings screen 6 3 2 4 Display Calibration Curve Completed calibration curves can be viewed graphically by pressing the function key below the Show curve option 29 6 4 CREATING A NEW STANDARD CURVE The user must firstly edit the calibration table before attempting to create a new standard curve 30 110 Hmm Abs Insert the cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the first standard solution into the sam
2. and down arrow keys Press the Enter key to confirm 25 5 2 3 Selecting the Unit of Measurement The unit of measurement can be selected by pressing the function key below the Unit option The required unit of measurement can then be selected from the available options of IU mM L M L ug mL mg mL mg L mEq ppb ppm and other Press the Enter key to confirm 5 2 4 Entering a Concentration Factor The concentration of a sample can be determined by WL 448 Hun I 16346 using an appropriate multiplication factor with the 0 014 mg L measured photometric reading A concentration factor can be entered by pressing the function button below the Factor option The required factor can then be entered using the numerical keypad Press the Enter key to confirm 9 3 CALIBRATION 5 3 1 Zero Calibration UL 448 8nm A zero calibration can be performed at the same 0 000 Abs wavelength at which the sample will be measured With both the reference a sample positions empty and the sample chamber lid closed press the 100 T OAbs button to zero the instrument s photometric reading 24 5 3 2 Calibrating to a Standard 03 40 5A HD A calibration standard can be used to determine the concentration factor Insert the cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the standard solution into the sample position Close the instrument lid The
3. can be smoothed by pressing the function key below the Smooth option A result file can be saved to the instrument s internal memory Press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 35 7 9 O O CH Jo 230 0 Open 36 RECALLING STORED RESULTS Vavelength nm A result file can be opened from the instrument s internal memory Press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm SECTION 8 KINETICS The kinetics measurement mode enables the absorbance or transmittance of an active molecule to be measured over a period of time for example enzyme analysis of horseradish peroxidase The absorbance or transmittance is measured at regular time intervals at a set wavelength over a period of time The results are plotted on a graph to show the change in absorbance or transmittance over time Following sample measurement statistical analysis of all or part of the experiment c
4. instrument keypad The Esc key can be used to return to the previous menu without saving any changes 4 2 MAIN MENU SCREEN UL 440 Gnn VIT The main menu screen provides access to all measurement modes the system utility menu EE JENWAY 6850 and the variable slit width setting The available Main Menu Photometrics Mode i Quantitsation Mode measurement modes are photometrics quantitation spectrum scan kinetics ectrum Scan tics Mode AA a DNA Protein and multi wavelength ti Wavelenagth Mode The system utility menu option allows the user to perform a variety of setup and service functions see section 4 3 for further details on these Main Menu Screen options 4 2 1 Slit Width Setting The slit width option allows the user to select the instrument s bandwidth parameter from the available options 0 5 1 0 2 0 4 0 and 5 0 nm Use the arrow keys to cycle through the options and press the Enter key to confirm The instrument will perform a dark current calibration before returning to the main menu 18 4 3 SYSTEM UTILITY MENU The system utility menu option allows the user to WL 238 8nm Bi gt perform a variety of setup and service functions m 6850 1 Wavelength calibration ce Routines PET Reset rinte Setu 2 Edit printer settings 3 Edit lamp settings 4 Edit time and date settings 5 Measure the instrument s dark current 6 Connect to a PC 7 Edit keypad sound
5. key to confirm 8 4 SAMPLE MEASUREMENTS 8 5 38 DD 8nm Abs Time s setup Mode Process Search ES XScale scale Time s 31 Setup Mode Process Search Insert a cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and press the Start Stop key on the keypad Once the measurement is complete the measured kinetics scan will be displayed on the screen ADJUSTING THE DISPLAYED SCAN RANGE The instrument will display the full range scan after a measurement is complete To change the displayed time range press the left or right arrow keys The start and end times can be entered using the numeric keypad Press the Enter key to confirm To change the displayed photometric range press the up or down arrow keys The minimum and maximum values can be entered using the numeric keypad Press the Enter key to confirm The kinetics scan will then be shown with the updated range values The return to the full kinetics scan the user will need to re enter the original time and photometric range values 8 6 8 7 tup Mode Process 5 CALCULATING I U CONCENTRATION Tim 38 8 Abs Time s Start Timwe 10_ CH CW 0 Time s 30 SPECTRUM SMOOTHING Time s SI EER VERE FER e Press ESC to quit search mode The instrument s
6. manutenzione e riparazione deve essere effettuata sulla base di quanto indicato nel presente manuale da personale qualificato consapevole dei rischi connessi E fondamentale che il personale operativo e il personale addetto alla manutenzione utilizzino un sistema di lavoro sicuro oltre a seguire le istruzioni specificate nel presente manuale Oltre a quelli indicati nelle procedure di manutenzione all interno di questo dispositivo non sono presenti altri elementi sui quali possibile effettuare interventi La rimozione delle protezioni e qualsiasi tentativo di regolazione o di manutenzione posto in essere da personale non qualificato invalider la garanzia In questi casi sar necessario pagare un importo per le riparazioni effettuate 4 E sempre necessario fare riferimento ai dati sulla salute e sulla sicurezza forniti con le sostanze chimiche utilizzate Adottare le procedure di laboratorio generalmente accettate per la gestione delle sostanze chimiche 5 Nel caso in cui si sospetti che la salute possa essere pregiudicata in qualsiasi modo disattivare l unit per renderla inutilizzabile Qualsiasi condizione di errore deve essere immediatamente segnalata al responsabile per la manutenzione Lea esta informaci n atentamente antes de instalar o utilizar este equipo 1 La unidad descrita en este manual est dise ada para que solamente la utilice personal con formaci n Cualquier operaci n de ajuste mantenimiento y repar
7. options 8 Set the displayed language 9 Perform a system baseline measurement 10 Perform an instrument reset System Utility Menu 4 3 1 Wavelength Reset Highlight the wavelength reset option and press the Enter key to confirm This will recalibrate the wavelength setting of the instrument Once complete the instrument will return to the System Utility menu 4 3 2 Printer Setup In the printer setup menu the user can perform the WI 30 Hmm I JENWAY Y 6850 up Printer CEE ne t rit Port Select rinter following tasks 1 Reset printer Select print output port Comm or Lpt node 2 3 Select printer type 4 Change print mode Report or Screen Shot Highlight the required option using the up and down arrow keys and press the Enter key to confirm 19 4 3 3 Lamp Setup In the lamp setup menu the user can perform the WL 38 Bnm i JENWAY ENWAY 6851 0 following tasks Leaps Management 1 Switch on off the deuterium lamp u Reset D2 Le mp usage time 2 Reset the deuterium lamp usage timer 3 Switch on off the tungsten lamp 4 Reset the tungsten lamp usage timer 5 Change the lamp switch point Highlight the required option using the up and down arrow keys Press the Enter key to confirm 4 3 3 1 Change the Lamp Switch Point Highlight the Change the lamp switch point option and press the Enter key to confirm This will allow the user to enter the wavelength where the instrument wil
8. regular calibration checks and a suitable Quality Control QC programme The sample chamber lid must be fully closed during measurement and before any readings are recorded or printed The correct selection of sample containers is imperative for accurate and reproducible results a b c d e f g Check that the material of the sample container is compatible with the wavelengths to be used for measurement In general glass can only be used down to 360nm or 320nm depending on quality Standard plastic cuvettes can be used down to 320nm Special UV versions can be used down to 260nm Below this level quartz cuvettes must be used Plastic disposable cuvettes should only be used ONCE Glass cuvettes should be thoroughly cleaned after use Discard when scratches become evident on optical surfaces Care should be taken when selecting semi micro or micro cuvettes The cuvette window on the inner chamber the area filled with sample must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample In this case semi micro or micro cuvettes with self screening black surrounds must be used or alternative holders for these cuvettes should be used Glass test tubes and other sample tubes should be used with care Where possible matched tubes should be used and any index mark set to the correct position before measurements are made Ensure any sample conta
9. using the numeric keypad Press the Enter key to confirm 9 5 SELECTING THE UNIT OF MEASUREMENT The unit of measurement can be selected by pressing the function key below the Unit option The required unit of measurement can then be selected from the available options of IU mM L M L ug mL mg mL mg L mEq ppb ppm and other Press the Enter key to confirm 42 9 6 RESET MODE SETTINGS The DNA Protein mode settings can be reset to their default values by pressing the function key below the Default option 9 7 DNA PROTEIN MEASUREMENTS Ensure that a cuvette containing the blank solution is in WL 328 8nm Abs 4 12 ki m DNA Protein Test i ee the reference position in the sample chamber and insert the cuvette containing the sample solution into Aref un the sample position Close the instrument lid and pres en we pe p P 0 512 E r the Start Stop key on the keypad Once the ee measurement is complete the determined results will be displayed in the DNA Protein mode screen Further samples can be measured in a similar manner 9 8 STORING METHODS RESULTS A method result file can be saved to the instrument s 126 Onm Abs 1 14 internal memory DNA Protein Test Ta In the DNA Protein mode screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers
10. 2 Abs 0 5 to 1 0 Abs Resolution 0 1 T 0 001A Stray light Noise Stability 0 001A at 500nm after 15min warm up Range Data points Calibration Units Curve fit algorithms Multi wavelength Range Data points Measurement Time Up to 12 hours Scan Interval 0 1 0 2 0 5 1 2 5 10 or 30 seconds Calibration Blank with a single standard or factor Display Graphical and concentration Analysis Slope and line of best fit between any two points Range Any range between 190 to 1100nm Scan speed 100 to 2000nm min Scan interval Selectable 0 1 0 2 0 5 1 2 or 5nm DNA RNA Ratio concentration A320 correction modes Measurement Direct UV SECTION 2 Installation 2 1 UNPACKING Remove the 6850 from the packaging and ensure the following items are included 1 Model 6850 spectrophotometer fitted with 10 x 10mm cuvette holder 685 SC 2 Power supply cables HH179 S UK lead HH180 S EU lead CABLEUS US lead 3 Instruction manual 685 030 4 10mm Glass Cuvettes x 4 035 027 5 10mm Quartz Cuvettes x 2 035 028 6 Dust Cover 685 040 7 PC software CD and USB security dongle 685 035 2 2 INSTALLATION The 6850 is supplied ready to use The working temperature and humidity range of the 6850 spectrophotometer is 15 35 C 15 70 relative humidity The storage temperature and humidity range of the 6850 spectrophotometer is 10 50 C 15 70 relative humidity The unit should be placed on a clean fla
11. 37 8 1 KINETICS MODE SCREEN cia ae 37 8 2 WAVELENGTH SELECTION enee 37 8 3 METHOD SETUP se seen ee ee 37 8 3 1 Selecting the Measurement Mode ss 38 8 4 SAMPLE MEASUREMENTS SS een 38 8 5 ADJUSTING THE DISPLAYED SCAN RANGE 52 sun 38 8 6 CALCULATING NEEN TEL IEN eege 39 8 7 SPECTRUM SMOOTHING sense euer 39 8 8 STORING RESULTS nassen ati td 40 8 9 RECALLING STORED RESULTS 2222 22mm 2m 2 ee 40 SECTION 9 DNA PROTEHN 2 2222200220020000000n0nnun nun nnnnnnnnnnn nun anna nun ann 41 9 1 BNA MENU OP HON S seele 41 9 2 ADJUSTING THE MEASUREMENT MODE iii 41 9 3 WAVELENGTH SELECTION aci dla 42 9 4 ADJUSTING THE CONCENTRATION CALCULATION FACTORS es 42 9 5 SELECTING THE UNIT OF MEASUREMENT ire 42 9 6 RESET MODE SETTINGS EE 43 9 7 DNA PROTEIN MEASUREMENTS ci a 43 9 8 STORING lag e TE RE 43 9 9 REGALEINGSTOREDRESUETS sans sus ea 44 SECTION 10 MULTI WAVELENGTH 2 2 2 2202202020002000n00n0n ann nun nun nun nun nun nnnn 45 10 1 MULTI WAVELENGTH MODE OPTIONS iii 45 10 2 WAVELENGTH SELECTION ES en 45 10 3 SAMPLE MEASURE MEIN Tossa oo 46 10 4 STORING METHODS IRESULT S cuco diese es tertele 46 105 REGALONG STORED RESULTS uniera 46 SECTION 11 ACCESSORIES AND SPARE PARTS 47 11 1 OPTIONAL ACCESSORIES EE 47 11 2 INSTALLING THE PASSIVE AND ACTIVE ACCESSORIES cooccocccccncnccnccnccnncnononononannnnnons 47 11 2 1 Passive Accessories E 47 11 2 2 ACUIVE Accessories une ae 48 11 2 2 1 8 Position Automa
12. ACEMENT Warning HOT Switch off the mains power supply and wait at least 20 minutes before attempting to replace the tungsten or deuterium lamps 12 2 1 Tungsten Lamp Replacement 1 Unscrew the 4 screws indicated 2 screws on each side and remove the cover of the spectrophotometer 2 Unscrew the 2 screws on the top of the lamp chamber and remove the cover 51 ns E ry A Lamp Tungsten Entrance Hole S 3 Whilst wearing cotton gloves remove the defective tungsten lamp and replace with a new one Switch on the power and move the Switch Mirror down into the position shown in the picture opposite Observing the entrance window on the instruments monochromator adjust the tungsten lamp until the light is centred on the entrance hole Disconnect the power and re attach the lamp compartment cover and the top case of the spectrophotometer Unscrew the 4 screws on the spectrophotometer s top case see 12 2 1 and remove the cover of the spectrophotometer Unscrew the 2 screws on the top of the lamp chamber and remove the cover see 12 2 1 Unscrew the 2 screws on the deuterium lamp flange No 1 unplug the connector on the power board No 2 and remove the deuterium lamp Whilst wearing cotton gloves replace with a new deuterium lamp Re fix the 2 screws and reconnect the power cable Re attach the lamp compartment cover and the top case of the spectrophotomete
13. JENWAY Operating Manual Bibby Scientific 685 030 REV A 03 12 Safety Please read this information carefully prior to installing or using this equipment 1 The unit described in this manual is designed to be operated only by trained personnel Any adjustments maintenance and repair must be carried out as defined in this manual by a person qualified to be aware of the hazards involved It is essential that both operating and service personnel employ a safe system of work in addition to the detailed instructions specified in this manual Other than for those items defined in the maintenance procedures herein there are no user serviceable items in this instrument Removal of covers and attempted adjustment or service by unqualified personnel will invalidate the warranty and may incur additional charges for repair References should always be made to the Health and Safety data supplied with any chemicals used Generally accepted laboratory procedures for safe handling of chemicals should be employed If it is suspected that safety protection has been impaired in any way the unit must be made inoperative and secured against any intended operation The fault condition should immediately be reported to the appropriate servicing authority Merci de lire attentivement ces informations avant d installer ou d utiliser cet appareil 1 L appareil d crit dans ce manuel est con u pour tre utilis uniquement par de
14. RT Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to help with any applications advice and questions you may have about our products and how to use them If you require any technical or application assistance please contact the team at E mail jenwayhelp bibby scientific com Phone 44 0 1785 810433 Fax 44 0 1785 810405 54 SECTION 14 DECLARATION OF CONFORMITY Declaration of Conformity AE RER ER EE E A TRE EE UV Visible Spectrophotometer Model 6850 This product complies with the requirements of the EU Directives listed below 2004 108 EC EMC Directive 2006 95 EC Low voltage Directive LVD Compliance with the requirements of these Directives is claimed by meeting the following standards EN 61326 1 2006 Electrical Equipment for Measurement Control and Laboratory use EN 61010 1 2001 Safety Requirements Electrical Equipment for Measurement Control and Laboratory use CE mark affixed 2012 dll er je Signed VA mr Mr S Marriott Date II 2 IZ Authority Technical Director Bibby Scientific Ltd Bibbv Scientific Bibby Scientific Ltd Stone Staffs ST15 OSA UK Tel 44 0 1785 812121 Fax 44 0 1785 813748 55
15. aci n debe llevarse a cabo del modo indicado en este manual y debe realizarla una persona cualificada que sea consciente de los peligros que implica 2 Es fundamental que tanto los operarios como el personal de servicio utilicen un sistema de trabajo seguro as como las instrucciones detalladas que se especifican en este manual 3 Cualquier elemento que no se encuentre entre los definidos en los procedimientos de mantenimiento aqu descritos no podr utilizarse en este instrumento La extracci n de las tapas y los intentos de ajuste o reparaci n por parte de personal no cualificado invalidar n la garant a y pueden incurrir en cargos adicionales por reparaci n 4 Siempre deber an consultarse los datos sobre Salud y Seguridad que se suministran con cualquier producto qu mico que se utilice Es necesario llevar a cabo los procedimientos de laboratorio de aceptaci n generalizada para la manipulaci n segura de productos qu micos 5 Si existe la sospecha de que las medidas protectoras de seguridad han quedado da adas en cualquier modo la unidad debe inutilizarse y protegerse contra toda operaci n que se intente llevar a cabo El estado de fallo debe comunicarse inmediatamente a la autoridad de servicio de mantenimiento y reparaci n pertinente Contents SECTION T Introduction es E 8 1 1 INSTRUMENT DESCRIPTIONS ae 8 1 2 INSTRUMENT SPECIPIGATION cosas acacia 8 SECTION 2 Installation sia ds 9 2 1 UNPACKING seen ee ee e
16. an be performed 8 1 KINETICS MODE SCREEN UL 600 8nm Abs TT The kinetics measurement mode enables measurement parameters to be changed and post measurement tools to be accessed The four options at the bottom of the screen allow the user to edit the kinetics run settings select the measurement mode perform a linear regression analysis on the data and perform a post measurement search of the scan s data points These options are selected with the corresponding function buttons Operating Menu 8 2 WAVELENGTH SELECTION The wavelength can be adjusted by pressing the GOTOA key on the instrument keypad The required wavelength can then be entered using the numerical keypad Press the Enter key to confirm 8 3 METHOD SETUP The kinetics scan settings can be edited by pressing UL GH Hop Abs the function key below the Setup option The options that can be changed are 1 Run Time 2 Delay Time 3 Timeis 3 3 Time Interval Run Time 30 The numeric keypad should be used to enter the required run and delay times and the up and down arrow keys are used to select from the available time interval options Press the Enter key to confirm 37 8 3 1 Selecting the Measurement Mode The measurement mode can be selected by pressing the function key below the Mode option The required measurement mode can then be selected from the available options of Abs and T using the up and down arrow keys Press the Enter
17. ance is inversely related to transmittance A log 1 y 14 3 2 NUCLEIC ACID DETERMINATION DNA RNA and oligonucleotides can be measured directly in aqueous solutions in a diluted or undiluted form Aqueous buffers with low ion concentrations e g TE buffer are ideal for this method The concentration is commonly determined by measuring at 260nm against a blank and then evaluating against a factor The 6850 has pre defined methods installed which assume that absorption of 1 OD A is equivalent to approximately 50ug ml dsDNA 37ug ml ssDNA 40ug ml RNA and 30ug ml for oligonucleotides DNA interference by contaminants can be assessed by the calculation of an absorption ratio The ratios A260 A280 and A260 A230 are used to estimate the purity of nucleic acids since proteins absorb at 280nm and substances such as peptides phenols aromatic compounds or carbohydrates absorb at 230nm Pure DNA should have an A260 A280 ratio of approximately 1 8 and pure RNA 2 0 In pure nucleic acid samples the A260 A230 ratio should be approximately 2 2 Nucleic acid concentration can also be estimated with the following calculations Conc ug ml Abs 260nm x 62 9 Abs 280nm x 36 0 Conc ug ml Abs 260nm x 49 1 Abs 230nm x 3 48 Referring to a blank value where no absorption should occur is commonly required On the 6850 the default reference wavelength is 320nm and the user can include the measured absorbance value in all nu
18. and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 43 9 9 RECALLING STORED RESULTS A method result file can be opened from the Cp tinm Abs DNA Protein Test 2 m instrument s internal memory In the DNA Protein mode screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm If more than one result is stored in a file the up and down arrow keys can be pressed to scroll though the results Alternatively the user can press the left or right arrow key to enable a search option The number of the result that is to be viewed is entered using the numeric keypad Press the Enter Key to confirm 44 SECTION 10 MULTI WAVELENGTH The multi wavelength measurement mode allows the user to measure a sample s photometric absorbance or transmittance at up to 10 wavelengths Following sample measurement the photometric readings are displayed on the multi wavelength mode screen 10 1 MULTI WAVELENGTH MODE OPTIONS 198 0nm Abs Multi Wavelength Test 1 WL 10 2 WAVELENGTH SELECTION WL 198 6nm Abs Setup Wavelength B5elect The multi wavelength measurement mode enables measu
19. ange scan after a measurement is complete To change the displayed wavelength range press the left or right arrow keys The minimum and maximum display wavelengths can be entered using the numeric keypad Press the Enter key to confirm To change the displayed photometric range press the up or down arrow keys The minimum and maximum values can be entered using the numeric keypad Press the Enter key to confirm The spectrum scan will then be shown with the updated range values To return to the full spectrum scan the user will need to re enter the original wavelength and photometric range values 7 6 SPECTRUM SEARCH 7 7 SPECTRUM SMOOTHING 650 Bnm Abs nboothing Spectra res i A 230 0 Vavelength nm 650 si Setup Mode Search Smooth 7 8 STORING RESULTS WL GH Hmm Abs D ER ol input File Nawe SPECTRUM_ The photometric values of the measured spectrum can be viewed by pressing the function key below the Search option The user can press the up and down arrow keys to move between the peaks in the scan that are above the threshold peak height The user can alternatively press the left or right arrow keys to review each point in the scan in turn The peak height threshold can be adjusted by pressing the function key below the PK Height option The threshold peak height value can be entered using the numeric keypad Press the Enter key to confirm The measured spectrum scan
20. apply a smoothing algorithm to the displayed scan These options are selected with the corresponding function buttons The spectrum scan settings can be edited by pressing the function key below the Setup option The options that can be changed are 1 Scan from Highest Wavelength 2 Scan to Lowest Wavelength 3 Scan Interval 4 Scan Speed The numeric keypad should be used to enter the required start and end wavelengths and the up and down arrow keys are used to select from the available scan interval and scan speed options Press the Enter key to confirm 33 7 3 SELECTING THE MEASUREMENT MODE The measurement mode can be selected by pressing the function key below the Mode option The required measurement mode can then be selected from the available options of Abs and T using the up and down arrow keys Press the Enter key to confirm 7 4 SAMPLE MEASUREMENTS wegl mem 30 0 Vavelength nm 650 Setup Mode Search Smooth Insert a cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and press the Start Stop key on the keypad Once the measurement is complete the measured spectrum scan will be displayed on the screen 7 5 ADJUSTING THE DISPLAYED SCAN RANGE bob Bnm Abs 230 0 Vavelength nm Min X 500_ 34 The instrument will display the full r
21. asy and effective way of navigating the different measurement modes entering numbers saving and analysing results The keypad is displayed below E er E E os CS ot ERT 100 T SAVE A OAes a GoTo PRINT CELL STOP h d Fig 2 4 1 Keypad Function Keys Allows on screen options to be selected Numeric Keys Enter numbers and letters Select u corresponding menu options CLEAR CLEAR Key Delete the entered value or stored data BACK Key Delete a single character 11 ESC ESC Key Return to previous menu screen 100 T 0ABS 100 T OAbs Key Zero Blank instrument OPEN Key Open results stored in internal memory SAVE SAVE Key Save results to internal memory START START STOP Key Start Stop measurement GOTO Key Set the instrument wavelength using the numeric BON keys Press the Enter key to confirm PRINT PRINT Key Print result ENTER ENTER Key Confirm operation CELL Key Select Deselect Auto cell Holder position If fitted CELL Use the numeric keys 1 8 to move to cell holder to the corresponding cell position o O g RIGHT LEFT Keys Search peak valley and set X axis scale CAN d UP DOWN Keys Scroll menu data and set Y axis scale 2 5 REAR PANEL The image below shows the rear panel of the instrument o gt oF Een hy gt 2 6 Fig 2 5 1 Rear Panel Fan Cover Allows access to lamp when replacement is necessary Power switch On off swit
22. ble screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm The user should ensure that the appropriate standard curve data has been measured or recalled and that the instrument is displaying the quantitation mode screen before performing a sample measurement 31 6 7 1 Quantitation Measurements 146 Hun Abs Quantitation Mode Abs Conc mg L mA Ee z S SER T 2 166 ROL ees SB AS 6 8 STORING RESULTS 148 Gnm Abs Quantitation Mode lease input File Name 5TDRE 6 9 RECALLING STORED RESULTS WL 448 8nm Abs 05949 2947A Der SIEHT DRES gus E 32 16 24 1 D2 m H E UL nm 440 0 Ensure that a cuvette containing the blank solution is in the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and pres the Start Stop key on Once the measurement is complete the determined result will be the keypad displayed in the quantitation mode screen Further samples can be measured in a similar manner A result file can be saved to the instrument s internal memory In the quantitation mode screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 chara
23. c MeasuremeNiS dented os del De di E E 25 5 4 2 Measuring a Sample after Entering a Concentration Factor aennsesennenennnennnnenrnnnnennee 25 5 4 3 Measuring a Sample after Calibrating to a Standard nnnnannnnennnnnennnnnnnennnnnnsnnnnsrnnnennne 26 SECTION 6 QUANTITATION ee 27 6 1 QUANTITATION MODE SCREEN ii a aa aaa nennen 27 6 2 WAVELENGTH SELEECTION euer del 27 6 3 METHOD SE FUP 4 e a 27 6 3 1 Selecting the Unit of Measurement ss 28 6 3 2 Quantitation Table Settings sise 28 6 3 2 1 Sleet CUIV G F shone detains bl 28 6 3 2 2 Manually Enter Curve Constant ccccccecceccseeeeeceeeeecaeeeeesseeeeeeseaeeesaaeseesaeeeesaees 29 6 3 2 3 Edit Calibration Table ui ee 29 6 3 2 4 Display Calibration Curve an sen 29 6 4 CREATING A NEW STANDARD CURVE ae aaa 30 6 5 STORING A STANDARD CURVE otra nadaa da beste 31 6 6 RECALLING A STORED STANDARD CURVE ire 31 6 7 SAMPLE MEASUREMENTS Asse a a a a 31 6 7 1 Quantitation Measurements A 32 6 8 STORING RES URSS a isa 32 6 9 REGALEING STORED RESULTS 32 SECTION 7 SPEETRUM u 33 7 1 SPECTRUMIMODE SCREEN GE ers ee sc 33 7 2 pei KO SS aah ee a a 33 7 3 SELECTING THE MEASUREMENT MODE Ne 34 7 4 SAMPEE MEASUREMENTS seele 34 7 5 ADJUSTING THE DISPLAYED SCAN RANGER iii 34 7 6 SPEGTRUM SEAN Estat rs a ea ne ee eee emer 35 1 7 SPECTRUM SMOO TING ass nee a ea 35 1 8 STORING RESULTS ie ds 35 7 9 RECALLING STORED RESULTS wipe eee ad 36 SECTION i KINETICS ise ea cece rs wc wre o een
24. ch for the unit Power in socket Connection socket for power supply unit LCD contrast adjustment Connection to a PC or external serial printer Parallel port Allows the accessory printer to be connected USB port Allows the instrument to be connected to a PC FRONT VIEW The image below shows the front view of the instrument ee gt D Fig 2 6 1 Front Panel LCD display Keypad Sample chamber lid Sample chamber 13 SECTION 3 THEORY AND PRACTICE OF SPECTROSCOPY MEASUREMENTS 3 1 THEORY OF SPECTROSCOPY MEASUREMENT UV visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample This is used to identify the presence and concentration of molecular entities within the sample The Beer Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through The Beer Lambert law states that A elc A is the absorbance E is the molar absorption coefficient I mol cm c is the concentration mol I is the path length cm This law shows that absorbance is linear to concentration but this is only true for low concentrations For absorbance levels above 3 the concentration starts to move away from the linear relationship Transmittance is the proportion of the light which passes through the sample Where lo is the incident light k is the transmitted light L is the path length L Therefore T h lo Absorb
25. cleic acid calculations The default wavelength can be modified from 320nm if required 3 3 SPECTROSCOPY MEASUREMENT There are four main components of a spectrophotometer These are a light source to emit a high and constant amount of energy over the full wavelength range a method for separating the light into discreet wavelengths a sample holder and a light detector The optical layout of the 6850 spectrophotometer is shown overleaf The light from the tungsten and deuterium lamps is focused onto the grating which separates the light into discreet wavelengths The diffracted spectrum of light then passes through a further slit and lens arrangement before passing through a beam splitter which directs half of the light towards to sample holder and half towards the reference sample holder The light which is not absorbed by the two solutions is transmitted through a collecting lens and onto the signal detector The signal from each photo diode detector is used to calculate the transmittance The result is displayed either as transmittance or absorbance on the instrument display 15 3 4 16 GOOD PRACTICE GUIDELINES 1 For optimum performance all spectrophotometers should be sited in a clean dry dust free atmosphere When in use ambient temperature and light levels should remain as constant as possible If required adherence to Standard Operating Procedures SOP and Good Laboratory Practice GLP should be monitored with
26. concentration of the calibration standard is entered by pressing the function button below the Standard option The concentration of the calibration standard can then be entered using the numerical keypad Press the Enter key to confirm The concentration factor is then updated on the screen 9 4 SAMPLE MEASUREMENT 5 4 1 Photometric Measurements Insert the cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and the photometric result will be shown on the screen 5 4 2 Measuring a Sample after Entering a Concentration Factor WL 440 0nm 5 694 mg L Factor Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample chamber Close the instrument lid and the concentration value will be shown on the screen In order to measure a sample based on a known factor the value for the factor must be entered in the settings before commencing measurement of the sample 25 5 4 3 Measuring a Sample after Calibrating to a Standard Remove the cuvette containing the standard sample WL 448 8nm I 8615 and place a cuvette containing the sample to be 35 99 mg L measured in the sample chamber Close the instrument lid and the calculated concentration value will be shown Factor 63 28 Standard Conc 58 88 on the scree
27. cters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm A result file can be opened from the instrument s internal memory In the quantitation mode screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm SECTION 7 SPECTRUM The spectrum measurement mode enables measurements of absorbance or transmittance over a range of wavelengths to be performed The absorbance or transmittance at each wavelength is plotted graphically Post measurement tools such as peaks and spectral points analysis can be performed This operating mode can be used to partially characterise a sample 7 1 SPECTRUM MODE SCREEN 440 finm Abs YScale Ay sc LES rth Sesrch smooth 30 0 AFA ength 7 2 METHOD SETUP WL 448 8nm Abs The spectrum measurement mode enables measurement parameters to be changed and post measurement tools to be accessed The four options at the bottom of the screen allow the user to edit the scan settings select the measurement mode perform a post measurement search of the spectrum or
28. e available are 1 Absorbance Difference 1 2 Absorbance Difference 2 The default equations and factors used by the instrument are shown below Absorbance Difference 1 Purity Ratio Abs 260nm Abs 280nm DNA Conc ug ml Abs 260 x 62 9 Abs 280 x 36 0 Protein Conc yg ml Abs 280 x 1553 Abs 260 x 757 3 4 Absorbance Difference 2 UL 328 Hun Abs Purity Ratio Abs 260nm Abs 230nm RNA Conc ug ml Abs 260 x 49 1 Abs 230 x 3 48 Protein Conc ug ml Abs 230 x 183 Abs 260 x 75 8 The option to include the third wavelength as a reference is given after the measurement mode is selected Use the up and down arrow keys to select Yes or No and press the Enter key to confirm Selecting the Yes option will result in all the absorbance value measured at wavelength 3 being subtracted from the photometric values measured at wavelengths 1 and 2 9 3 WAVELENGTH SELECTION The measurement wavelengths of the selected measurement mode can be adjusted by pressing the GOTO key on the instrument keypad The required wavelengths can then be entered using the numerical keypad Press the Enter key to confirm 9 4 ADJUSTING THE CONCENTRATION CALCULATION FACTORS The measurement mode option can be selected by ez UL 338 8nm Abs 14 06 20 i i er pressing the function key below the Coeff option D2 aa D ee 1 The new concentration calculation factors can be WL num 260 0 entered
29. ell accessory and align the accessories connector with the instrument connection socket 48 Fixing Screws a Place the accessory in the correct orientation and re tighten the two fixing screws to fix the 8 cell accessory in place 11 3 USING THE 8 POSITON AUTOMATIC CELL CHANGER When the automatic 8 cell turret is installed the current cell position is indicated below the lamp status icons in the top right hand corner of the screen To activate the accessory press the Cell key on the instrument keypad The indicated cell position will be highlighted on screen Each cell position is selected by pressing the corresponding digit on the numeric keypad e g position 6 is selected by pressing the number 6 key on the instrument keypad The instrument display will update to show the new automatic cell changer position Press the Cell key on the keypad to de activate the 8 position automatic cell changer 49 11 4 SPARES Part Code Description of Spare Part 685 020 Tungsten lamp 685 021 Deuterium lamp 50 SECTION 12 MAINTENANCE AND SETVICE 12 1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust The sample area should always be kept clean and any accidental spillage should be wiped away immediately To give added protection when not in use the unit should be disconnected from the mains supply and covered with the optional dust cover 12 2 LAMP REPL
30. en entered press the Enter key to confirm A result file can be opened from the instrument s internal memory Press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm SECTION 9 DNA PROTEIN The DNA Protein measurement mode allows the user to measure multi wavelength absorbance ratios such as 260nm 280nm and 260nm 230nm which are commonly used to estimate a protein or nucleic acid sample s purity The mode also includes calculations that can be used to estimate the concentration of the protein or nucleic acid sample 9 1 DNA MENU OPTIONS WI 130 nm Abs DNA Protein Test The DNA Protein measurement mode enables measurement parameters to be changed The four options at the bottom of the screen allow the user to edit the concentration calculation factors select the measurement mode select the units of the measurement and reset the modes settings to their default values These options are selected with the corresponding function buttons 9 2 ADJUSTING THE MEASUREMENT MODE Mosarch Ns roll EE sorbance difference L The DNA Protein measurement mode includes two sets of calculations to calculate the concentration of nucleic acid and protein samples The measurement mode option can be selected by pressing the function key below the Mode option The options that ar
31. i 9 2 2 INSTALLATION coa 9 2 3 DIS PNR ia 10 2 4 CONTROLS Eee ee han 11 2 5 ee 13 2 6 FRONT VIEW o dade aks 13 SECTION 3 THEORY AND PRACTICE OF SPECTROSCOPY MEASUREMENTS 14 3 1 THEORY OF SPECTROSCOPY MEASUREMENT asasnsnonnnnsnnnnnonnnnnnnrssrnrornrressrnrernrreenna 14 3 2 NUCLEIC ACID DETERMINATION E22 2 ehaai a 15 3 3 SPECTROSCOPY MEASUREMENT a 322 an o 15 3 4 GOOD PRACTICE GUIDELINE S Ess 228222 ae aa 16 SECTION 4 INSTRUMENT SETUP sense ann en eee eiuebeueds 18 4 1 INSTRUMENT NAVIGATION nn Hu a a a a a 18 4 2 MAIN MENU SCREEN zusehen en 18 4 221 Tee SSI se ls A 18 4 3 SYSIEM UTILITY MENU Sai ea ee 19 e er a Wavelengtii EE ER 19 43 2 let EE 19 4 33 A 20 4 3 3 1 Change the Lamp Switch Poli 20 4 34 A a eisen eiserne 20 4 3 4 1 A een 20 4 3 4 2 SER Dale Eege 21 4 3 4 3 Display Timer Date ias ii ua 21 24 39 Relresh Dark UI aida 21 2 36 CONECO EE 21 OT OO RS A er 21 2 3 8 EE een 21 4 39 Remeshioystem EE 21 4 3 10 Delete Entire Saved Files iii 22 4 311 Restore Default SENS still ee ua 22 SECTION 5 PHOTOMETRICS 22 23 5 1 PHOTOMETRICS MENU SCREEN rs aci 23 5 2 IAS TEE 23 82 1 Selecting a Wavelength DEE 23 5 2 2 Selecting the Measurement Mode 23 5 2 3 Selecting the Unit of Measurement sn 24 5 2 4 Entering a Concentration Factor suicidas een ea 24 5 3 CALIBRATION spell idolos iio 24 NoE a 24 5322 Calibrating to a Standard ult doo i 25 5 4 SAMPLE MEASUREMENT capota lo 25 54 1 Photometri
32. iners used are compatible with the constituents of both the samples and standards they are to hold Plastic cuvettes are not compatible with organic solvents All sample containers must be handled with care by the top bottom and non optical surfaces only Any finger marks evident must be removed by a suitable cleaning process 10 11 h Flow through cuvettes must be selected with care and consideration for the sample type sample volume pumping system rinse sample and waste handling to be used Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible If stored in stoppered and sealed cuvettes they should be filled with little or no air space and the values regularly checked against a reference standard or quality control material Samples should be allowed to equilibrate to ambient temperature before measurement unless a suitable temperature controlled sample holder is in use Temperature change during measurement may cause air bubbles to form on the walls of the sample holder This is a common cause of drift during measurement In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good quality deionised water or other suitable solvents must be used for dissolving or diluting samples chemicals and reagents All measurements requ
33. ire calibration to a blank for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample Where reagents are added to the sample to produce a colour proportional to its concentration a sample based blank should be used In this case the blank should consist of all reagents or chemicals to be used except the sample which will produce the colour to be measured Deviations from the Beer Lambert Law may occur at high and low concentrations giving non linear response during sample concentration measurements For all new methods a linear range should be defined by the preparation of a calibration curve The quantitation mode may be used to construct such a curve against which sample results are automatically measured Cuvettes and sample holders must be filled to a minimum level which covers the light path All Jenway spectrophotometers have a beam height of 15mm The instrument must be calibrated to zero absorbance 100 transmittance prior to taking readings In the spectrum measurement mode a baseline scan must be performed before performing a sample scan 17 SECTION 4 INSTRUMENT SETUP 4 1 INSTRUMENT NAVIGATION To navigate around the spectrophotometer screen press the arrow keys on the main keypad and use the Enter key to select the highlighted option Alternatively some options and actions require the user to press the function and numeric keys on the
34. l switch between the deuterium and tungsten lamps Enter a wavelength between 300 and 360 nm and press the Enter key to confirm Once complete the instrument will return to the Lamp Setup menu 4 3 4 Clock Setup In the clock setup menu the user can perform the WYL 298 Hmm JENWAY 6850 following tasks 1 Settime A zum Lin 2 Set date RE L 3 Display time 4 Display date Highlight the required option using the up and down arrow keys Press the Enter key to confirm 4 3 4 1 Set Time Highlight the set time option and press the Enter key to confirm This will allow the user to enter the current time using the numerical keypad and the decimal point keys Press the Enter key to confirm Once complete the instrument will return to the Lamp Setup menu 20 4 3 4 2 Set Date Highlight the set date option and press the Enter key to confirm This will allow the user to enter the current date in the format DD MM YY using the numerical keypad and the decimal point keys Press the Enter key to confirm Once complete the instrument will return to the Clock Setup menu 4 3 4 3 Display Time Date Highlight the display time or display date option and press the Enter key to confirm This will set the top right hand corner to show the selected option 4 3 5 Refresh Dark Current Highlight the refresh dark current option and press the Enter key to confirm This will re measure and store the detectors dark curren
35. mode enables simple measurements of absorbance transmittance and concentration to be performed In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor The sample is measured at one wavelength at one point in time There are no post measurement calculations available in this measurement mode 9 1 PHOTOMETRICS MENU SCREEN The photometrics measurement mode screen enables WL 448 ALL 0 000 Abs measurement parameters to be changed The four options at the bottom of the screen allow the user to select the unit of measurement the measurement mode the concentration factor and the calibration standard s concentration These options are selected with the corresponding function buttons 9 2 METHOD SET UP The photometrics measurement mode is very simple and the only parameters which can be adjusted are the wavelength the units of the measurement the measurement mode and the concentration setup parameters 5 2 1 Selecting a Wavelength The wavelength can be adjusted by pressing the GOTOA key on the instrument keypad The required wavelength can then be entered using the numerical keypad Press the Enter key to confirm 5 2 2 Selecting the Measurement Mode The measurement mode can be selected by pressing the function key below the Mode option The required measurement mode can then be selected from the available options of Abs T and Conc Factor using the up
36. n Me E SS ze Ka ep OF AE AGA KEPED OE ST Rector apa 26 SECTION 6 QUANTITATION The quantitation measurement mode enables sample concentrations to be calculated using a standard curve In this mode a number of standard solutions covering a range of known concentrations are measured The absorbance or transmittance of these solutions is plotted to create a standard curve Once the standard curve has been created a sample of unknown concentration can be measured and the concentration calculated using the standard curve 6 1 QUANTITATION MODE SCREEN ET The quantitation measurement mode enables A E measurement parameters to be changed The two options at the bottom of the screen allow the user to select the unit of measurement and edit the calibration standard settings These options are selected with the corresponding function buttons 6 2 WAVELENGTH SELECTION The wavelength can be adjusted by pressing the GOTOA key on the instrument keypad The user can select from the following options 1 Single WL 2 Isobestic where Abs Abs11 Abs12 m x AbsA1 n x AbsA3 3 3 Points where Abs AbsA2 m n m AbsA3 AbSA2 and n AbsA2 AbsA1 The required wavelength values can then be entered using the numeric keypad Press the Enter key to confirm 6 3 METHOD SETUP In this measurement mode the method setup parameters are accessed by pressing the function keys below the two optio
37. name max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 10 5 RECALLING STORED RESULTS WL 440 Onm Abs 13 23 06 Multi Wavelength Test 1 ML D2 um no WL nn q Search scroll Open file TI mul A method result file can be opened from the instrument s internal memory In the multi wavelength mode screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm If more than one result is stored in a file the up and down arrow keys can be pressed to scroll though the results Alternatively the user can press the left or right arrow key to enable a search option The number of the result that is to be viewed is entered using the numeric keypad Press the Enter Key to confirm 46 SECTION 11 ACCESSORIES AND SPARE PARTS 11 1 OPTIONAL ACCESSORIES Part Code 685 204 685 131 685 005 685 304 685 401 035 088 035 091 060 422 035 143 685 035 685 040 Description of Accessory 10 x 10mm path length cuvette holder Water heated 10 x 10mm single cuvette holder 10 to 100mm adjustable path leng
38. nd der Vorgaben in diesem Handbuch und von einer kompetenten Person die mit den damit verbundenen Gefahren vertraut ist durchgefuhrt werden Es ist wichtig dass sowohl das Bedienungs als auch das Service Personal zusatzlich zu den detaillierten Anweisungen in diesem Handbuch ein sicheres Arbeitssystem einsetzen Mit Ausnahme der Teile deren Wartungsverfahren in diesem Handbuch beschrieben sind enthalt dieses Gerat keine weiteren Teile die vom Benutzer gewartet werden konnen Das Entfernen von Abdeckungen und Versuche von hierf r unqualifiziertem Personal Anpassungen oder Wartungsarbeiten durchzuf hren haben zur Folge dass die Garantie verf llt und k nnen zus tzliche Reparaturkosten ausl sen Es ist jederzeit auf die sicherheitsrelevanten Daten s mtlicher verwendeter Chemikalien Bezug zu nehmen Allgemein anerkannte Labormethoden zum sicheren Umgang mit Chemikalien sollten eingesetzt werden Besteht der Verdacht dass die Sicherheitsvorrichtungen in irgendeiner Weise besch digt wurden muss das Ger t au er Betrieb genommen und gegen weiteren Gebrauch gesichert werden Die St rung sollte der zust ndigen Serviceeinrichtung unverz glich gemeldet werden Leggere attentamente queste istruzioni prima di installare o utilizzare il dispositivo 1 L unita descritta nel presente manuale e stata realizzata per essere utilizzata solo da personale che ha ricevuto l apposita formazione Qualsiasi operazione di regolazione
39. ns on the quantitation menu screen 21 6 3 1 Selecting the Unit of Measurement The unit of measurement can be selected by pressing the function key below the Unit option The required unit of measurement can then be selected from the available options of IU mM L M L ug mL mg mL mg L mEq ppb ppm and other Press the Enter key to confirm 6 3 2 Quantitation Table Settings HI 448 Hunn Abs Calibration table No onc mg L Abs L ws gt SEN 6 3 2 1 Select Curve Fit UI 446 Anm Abs Calibration table e 28 The quantitation table settings are accessed by pressing the function key below the Curve Fit option in the quantitation mode screen Four new options are available in the quantitation table screen 4 Method Change the curve fit algorithm 5 Params Enter linear regression constants 6 Standard Edit calibration table data 7 Show Curve Display calibration curve The curve fit algorithm can be selected by pressing the function key below the Method option Four curve fit options are available 1 Linear Fit 2 Square Fit Quadratic 3 Cubic Fit 4 Linear Fit Through Zero The required option can be selected using the up and down arrow keys Press the Enter key to confirm 6 3 2 2 Manually Enter Curve Constants The user can manually enter the calibration curve 440 Unm Abs Calibration table _ constants by pressing the function key below the aof conc 29
40. oftware allows the user to calculate the concentration of a substance from the difference between the photometric values at two time points in the displayed kinetics scan To enter the calculation parameters press the function key below the Process option and enter a Start Time an End Time and a Factor pressing the Enter key to confirm each value When all values have been confirmed the instrument will calculate the concentration value according to the following equation Conc I U Abs start time ADS end time X Factor The calculated concentration value will be displayed on the kinetics mode screen The photometric values of the measured kinetics scan can be viewed by pressing the function key below the Search option The user can press the up and down arrow keys to review each point in the scan in turn 39 8 8 STORING RESULTS Time s Please input File Name KINEIICS 8 9 RECALLING STORED RESULTS 40 WL 688 Gnm Abs Time s TEES TNETICS kin A result file can be saved to the instrument s internal memory Press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has be
41. ple position Close the instrument lid and press the start stop button Once the measurement is complete the instrument will ask for the second standard solution to be inserted Repeat the measurement procedure for each standard solution Once all standard solutions have been measured the new standard curve and curve equation will be shown The standard curve can be viewed graphically by pressing the function key below the show curve option on the calibration table screen 6 5 STORING A STANDARD CURVE WL 440 Gnm Abs Calibration table No Conc f i 5 t A standard curve can be saved to the instrument s internal memory to allow it to be recalled for future use In the calibration table screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the Same manner Once the filename has been entered press the Enter key to confirm 6 6 RECALLING A STORED STANDARD CURVE WL 449 Gnm Abs Calibration table ERC DS ULinn 440 0 TAFET ANDARD fit 6 7 SAMPLE MEASUREMENTS A standard curve can be opened from the instrument s internal memory to allow it to be re used for sample quantification In the calibration ta
42. r 123 SERVICE Our dedicated service team are on hand to help in the unlikely event that your Jenway equipment develops a fault Please contact them by one of the following means with a clear description of the problem E mail service bibby scientific com Tel 44 0 1785 810475 Fax 44 0 1785 810471 On occasion it may be necessary for your equipment to be sent back to our Service Department for repair In this case please contact the Service Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Certificate This is available as a downloadable pdf file at www jenway com or contact us and we will be happy to fax you a copy Please clearly mark the package for the attention of the Service Department and post to the following address Bibby Scientific Ltd Beacon Road Stone Staffordshire ST15 OSA United Kingdom All replacement parts are guaranteed for 1 year and where ever possible returned equipment is turned around in 10 working days 53 SECTION 13 TROUBLESHOOTING 13 1 TROUBLESHOOTING GUIDE Unable to achieve a reading when Ensure the correct cuvettes are being used so that light measuring a sample isn t being absorbed by the cuvette Ensure the sample isn t too dense that light is not transmitted through the sample Ensure the lamp is working 13 2 TECHNICAL SUPPO
43. rement parameters to be changed The two options at the bottom of the screen allow the user to edit the measurement wavelengths and change the measurement mode These options are selected with the corresponding function buttons The measurement wavelengths of the selected measurement mode can be adjusted by pressing the function key below the WL Setup option or the GOTOA key on the instrument keypad The required wavelengths can be entered using the numerical keypad pressing the Enter key to confirm each value Further values can be added by moving to a new line with the down arrow key Once all the required wavelengths have been entered press the ESC key to exit the wavelength selection screen 45 10 3 SAMPLE MEASUREMENT WL 448 8nm Abs 13 23 2 S Multi Wavelength Test 5 WL p2 Lee No WLinw er 10 4 STORING METHODS RESULTS 148 Gnm Abs Insert a cuvette containing the sample to be analysed into the sample chamber and press the key below the sample measurement icon The instrument will take a reading at each of the specified wavelengths the operating menu screen will then display the result of the selected measurement calculation and the photometric readings for each of the measured wavelengths A method result file can be saved to the instrument s internal memory In the multi wavelength mode screen press the Save key on the instrument keypad The instrument will ask the user to enter a file
44. s personnes form es Tout r glage maintenance ou r paration doit tre effectu comme d crit dans ce manuel par une personne qualifi e consciente des risques encourus Il est essentiel que les personnes utilisant et intervenant sur cet appareil respectent les regles de s curit de travail en plus des instructions d taill es pr cis es dans ce manuel En dehors des l ments d crits dans les proc dures de maintenance ci incluses cet appareil ne contient aucun element reparable par l utilisateur L enlevement des capots et les tentatives de r glage ou de r paration par des personnes non qualifi es invalide toute garantie et entraine un risque de frais de reparation suppl mentaires Toujours se r f rer aux fiches techniques de sant et de s curit accompagnant tout produit chimique utilis Respecter les procedures de laboratoire g n ralement accept es pour la manipulation en toute s curit des produits chimiques Si l utilisateur suspecte qu un probleme quelconque puisse mettre en cause la s curit l appareil doit tre rendu inop rant en emp chant son utilisation Communiquer la d faillance constat e au service de maintenance comp tent Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrustung sorgfaltig durch 1 Das in diesem Handbuch beschriebene Gerat darf nur von geschultem Personal bedient werden Alle Anpassungen Wartungsarbeiten und Reparaturen mussen entspreche
45. t surface which is free from drafts and vibrations The units are designed for operation on 110V 11V 60Hz 1Hz and 220V 22V 50Hz 1Hz AC Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket Turn the power on at the mains and switch the instrument on using the power switch on the rear of the instrument The instrument will initially perform several power on tests before displaying the warm up screen WL 2680 Hop I _JENWAY 6850 1 Wsit until 05 booted Warm up 15 MINUTES cao Fig 2 2 1 Instrument power on tests complete The power on tests comprise 1 Lamp test 2 Detector test 3 Ato D converter test 4 Grating position check 5 Dark current check 2 3 DISPLAY The instrument has an inclusive display which enables text and graphs to be displayed clearly Following successful completion of the power on tests and the instrument warm up time the main menu screen will be displayed WI 444 DI JENWAY 6850 IM Nenu FER sntitation Node trum Scan s Node rotein Mode IT a WSVeleng Fig 2 3 1 Display Main menu options 1 Photometrics measurement mode DNA Protein measurement mode Quantitation measurement mode Multi wavelength measurement mode Instrument settings menu Slit width oO N sa 2 3 Spectrum measurement mode 4 Kinetics measurement mode 10 2 4 CONTROLS The keypad used for this model enables an e
46. t values 4 3 6 Connect to PC Connect the interface cable to the USB type B port on the rear of the instrument and connect to a USB port on the PC The connecting COM port should be configured with the following settings 38400 baud 8 data bits No parity 1 stop bit Highlight the Connect to PC option and press the Enter key to confirm 4 3 7 Beeper On Off Highlight the beeper on off option and press the Enter key to confirm An audible sound will be heard each time an instrument button is pressed when this option is enabled 4 3 8 Language Selection Not currently available 4 3 9 Refresh System Baseline Ensure the sample chamber is empty highlight the refresh system baseline option and press the Enter key to confirm This will re measure and store the detectors signals over the full wavelength range of the instrument 21 4 3 10 Delete Entire Saved Files Highlight the delete entire saved files option and press the Enter key to confirm A warning will be shown on screen and the user will need to confirm this command again by selecting the Yes option with the up and down arrow keys This action will delete all saved results and methods from the instrument 4 3 11 Restore Default Settings Highlight the restore default settings option and press the Enter key to confirm This will reset all instrument parameters back to their original factory settings 22 SECTION 5 PHOTOMETRICS The photometrics measurement
47. th cuvette holder Micro cuvette holder 8 position automatic cell changer Visible calibration set UV Visible calibration set Moulded cuvette rack for 16 10x10mm cuvettes Pack of 100 disposable micro cuvettes 70pl Prism PC software CD and security dongle Dust cover 11 2 INSTALLING THE PASSIVE AND ACTIVE ACCESSORIES There are two types of accessories which can be fitted in the sample chamber passive or active accessories The range of passive accessories includes 10 x 10mm cuvette holders water heated cuvette holders adjustable path length 10 to 100 mm cuvette holders and micro cuvette holders The active accessory is an 8 position automatic cell changer The instrument must be turned off before any accessories are fitted 11 2 1 Passive Accessories Fixing Screws Unscrew the two fixing screws to undo the passive accessory Lift out the passive accessory To fit a different passive accessory place the accessory in the correct orientation and re tighten the two fixing screws to fix in place To replace the passive accessory with an active accessory refer to section 11 2 2 47 11 2 2 Active Accessories 11 2 2 1 8 Position Automatic Cell Changer Unscrew the two fixing screws to undo the passive accessory Lift out the passive accessory Fixing screws On the underside of the 8 cell accessory locate the accessories power supply and communication connector Accessory connector Take the 8 c
48. tic Cell Changer ss 48 113 USING THE 8 POSITON AUTOMATIC CELL CHANGER un 49 MANS RARES oi essen 50 SECTION 12 MAINTENANCE AND SETVICE ooccocccocconccncconconccnnconccnnconcnanonanons 51 12 1 ROUTINE MAINTENANCE ie mem Late ed ni mie 51 122 LAMP REPLACEMENT 251 RE ee M R eu a 51 12 2 1 Tungsten Lamp Replacement iii 51 12 2 2 Deuterium Lamp Hepnlacement iii 52 12 2 O A en 53 SECTION 13 TROUBLESHOOTING 0 8 54 13 1 TROUBLESHOOTING GUIDE 2 22 54 1352 TECHNICAL SUPPORT a een na 54 SECTION 14 DECLARATION OF CONFORMITY 2 2 220220202002000n00n0nnnnn0n0n 99 SECTION 1 Introduction 1 1 INSTRUMENT DESCRIPTION The 6850 is a variable bandwidth double beam UV visible spectrophotometer with an integrated user interface for local control This instrument has highly stable optics and two detectors that measure the sample and reference solutions simultaneously to optimise the accuracy of the measurement The 6850 has measurement modes for photometrics concentration multi wavelength spectrum scanning quantitation kinetics and DNA and Protein analysis The instrument is supplied with Jenway Prism PC software to allow full PC control of the spectrophotometer 1 2 INSTRUMENT SPECIFICATION A ss Range Resolution Accuracy 0 3nm at 0 5 and 1nm bandwidth 0 5nm at 2 4 and 5nm bandwidth Repeatability Spectral bandwidth Transmittance Absorbance Accuracy Reproducibility 0 001 Abs 0 to 0 5 Abs 0 00

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