VC-6T User`s Manual (Rev. 050816.1)
Contents
1. supplied MPP manifold The required tubing length is left to the discretion of the user but should be sufficient to allow for a short connection between the manifold and the sample chamber Attach the remaining blunt end syringe needles to one end of each section of PTFE tubing Prepare the MPP manifold to accept the PTFE tubing by first sliding a short length of PE 160 tubing over the input ports on the MPP manifold Now make a connection between the output port on each PTFE valve and one input port on the MPP manifold Connection should be made using the PTFE tubing described in step 7 Attach the Luer fitting to the valve and the open end to the manifold Run a short length of PE 160 tubing from the output port on the MPP manifold to the input port of sample chamber Finally connect the VALVE MANIFOLD to the VC 6 CONTROLLER using the attached cable Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 6 CONTROL DESCRIPTION Front panel The front panel of the VC 6 Valve Controller contains TTL inputs for each valve an associated 3 position toggle switch for manually setting the state of each valve and an LED displaying the active status for each valve There is also a 3 position toggle switch for selecting the command input mode and a power switch with power on LED Command input toggle A COMMAND INPUT TOGGLE SWITCH is provided for each of the 6 channels of the VC 6 Controller to a2. other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Avoid abrasive cleaners Stuck or leaky valves One possibility for a stuck valve is that the valve assembly was not completely flushed out at the end of the day While excessive salt build up can result in a stuck valve a more significant condition is the potential formation of small pits in the valve seals which will result in a leaky valve A valve bound with salt can be loosened by flushing with warm water to see if the crystals will dissolve or loosen ETOH may be used for sticky buildups and dilute acetic acid may be used to loosen any mineral deposits With the valve in the open position inject the selected cleaning solution into the flow path using a 10 cc syringe Inject so solution flows from the input to output ports Repeat as often as necessary usually 3 4 washes are sufficient Use an empty syringe to blow air through valve to remove any remaining cleaning solution from the valve interior If the valve remains stuck or continues to leak after repeated attempts of this cleaning procedure then the valve should be replaced Warranty The VC 6T Valve Control System is warranted to be free from defects in materials and workmanship for a period of two years from the date of shipment If a failure occurs within this period we will repair or replace the faulty component s at our discretion This war
3. VC 6T Manual Rev 050816 1 Warner Instruments Perfusion Valve Control System Model VC 6T Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800 599 4203 203 776 0664 203 776 1278 fax CE VC 6T Manual Rev 050816 1 2 Table of Contents ASA TO 4 COMPoneniS coins 4 SS 11 1 h AP NOIR 4 CONTROL DESCRIP TION u us 6 A O 6 Command Pul 102 OLS iii tetris TILI U E A Rear DAME OPOPARCAO PRO Du AT 7 TON QV CS ee ee ne ee else 7 A PP ee ee ee ee POW ET VOU TOG ee ee ee E A 7 INSTRUCTIONS FOR USE au anni 8 Flow OU CRIN a gress os oc css ccc o EE E 8 APPENDIX 22020040 TO 9 MEA EE E E A EE 9 A E A 9 SIMAO ENVIE ee T AEE E Oe E A ee ne ee 9 A cecnses si csesseeue cases eceuasseeresssssesevceeeaesosssteeusceueceusssiss 9 SDC CIC AGIONS ina 10 COPUMICATIONINS scassssaxsasssuseusssseneasscsusesssbeccecsacausetssssadesdsusausessstesdsssassusetsssedessdsausesiasacssesassedsnasssadsasesaeaesssiaces 11 Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 3 The VC 6T Valve Control System lies at the heart of a multi valve perfusion system designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers In addition its flexible design allows the VC 6T to be used in many applications not using Warner equipment Features include v PTFE valves v 6 channels individually controlled v Computer controllable The complete system inc
4. e time for delivery of the selected solution will be achieved by keeping the tubing length between the MANIFOLD and sample as short as possible Assembly 1 Begin assembly of the VC 6T by first attaching the VALVE BRACKET to the SUPPORT STAND Place the VALVE BRACKET near the base of the SUPPORT STAND as shown above 2 This is followed by attaching the SYRINGE HOLDER to the top of the SUPPORT STAND Place the SYRINGE HOLDER near the top of the SUPPORT STAND as shown above 3 Remove the plungers from the six supplied 60 cc syringes and place the syringes into the SYRINGE HOLDER Attach STOPCOCKS to each syringe Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 5 10 11 12 Cut six 6 pieces of PTFE tubing long enough to run from the stopcocks to the input ports on the PTFE valves Attach one 1 blunt end 18 gauge syringe needle to both ends of each length of PTFE tubing to provide Luer connection points Insert a short section of PE 160 onto the needle tip to facilitate a tight seal between the PTFE tubing and the syringe needle Using the Luer ended PTFE tubes formed in step 5 make a connection between the stopcock on each syringe to the associated input port on each PTFE valve Proper tubing lengths Luer connector attachments and tubing placements are shown in the montage above Cut six 6 pieces of PTFE tubing to run from the output ports of the PTFE valve to the
5. ipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut USA Signature Gah gria Full Name Burton J Warner Position President Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 13 WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers Harvard Apparatus is committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for those Harvard Apparatus products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring E and control instruments Most of Harvard Apparatus product
6. llow selection between internal and external commands Placing a COMMAND INPUT TOGGLE SWITCH into the on position drives the associated valve into its open state A lit LED indicates the open state of the valve In a similar manner placing a COMMAND INPUT TOGGLE SWITCH into the off position drives the associated valve into its closed state An unlit LED indicates the closed state of the valve TTL Inputs TTL inputs front panel BNC s are provided for external control for each valve channel Use of these inputs can allow for the simultaneous opening of more than one valve from a digital source e g a computer Placing a COMMAND INPUT TOGGLE SWITCH into the ext position activates the associated TTL BNC input A logic level low O V applied to the BNC places the associated valve into the closed state Correspondingly a logic level high 3 5 V places the associated valve into the open state A lit and an unlit LED indicates the open and closed states of the valve respectively Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 7 Rear Panel The rear panel contains the power input module with fuse an EVENT MARKER OUTPUT and a 15 pin D connector for the VALVE CONTROL BRACKET To Valves A 15 pin D type female connector is used to connect the cable from the VALVE BRACKET to the CONTROLLER Event Out The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned o
7. ludes a valve controller a valve bracket with PTFE valves connecting cable an MPP 6 manifold a support stand syringe holder six 60 cc syringes 25 feet of PTFE tubing 10 feet of PE 160 18 blunt ended 18 gauge syringe needles and 6 stopcocks The controller can independently regulate the function of up to six valves Individual valves can be controlled via manual switch an external analog signal or an external digital TTL signal An event marker pulse generated each time a valve is switched on is provided at the rear of the instrument for recording into your acquisition system Valve transitions opened or closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 4 SETUP Components Before beginning setup take inventory of the supplied components You should have a valve bracket with included PTFE valves a syringe holder a support stand 18 blunt end syringe needles stopcocks 6 PTFE tubing 25 ft PE 160 tubing 10 ft an MPP 6 port manifold and a VC 6 Valve Controller syringe syringe holder stopcock Teflon tubing VC 6 controller valve bracket support stand The VC 6T is designed to operate as a stopped flow device wherein the valve for each channel is either open allowing solution to flow or is closed In general the shortest respons
8. n At all other times the Event Marker output is low 0 V Power input module A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with a NEMA 5 15P connector is standard A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 5 x 20 mm fuses of the type indicated Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 8 INSTRUCTIONS FOR USE Flow Adjustment Flow rates can be adjusted by raising or lowering the reservoir holder as well as by adjusting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the specified height with the supplied PTFE tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min 30 cm 12 in 9 ml min 20 cm 8 in 5 ml min Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 9 APPENDIX Maintenance Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to manufacture the system If salt solution spills on the valve assembly it should be cleaned as soon as possible with a soft cloth dampened with a mild solution of detergent and water The exterior of the CONTROLLER may be cleaned periodically to remove dust grease and
9. ranty does not cover failure or damage caused by physical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Inc Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 Specifications Valve Bracket Valves Connection Cable Tubing Reservoirs Reservoir Holder Valve Controller Switch Selection External Input Event Marker Manifold Power Operating Temperature Dimensions H x W x D Weight Shipping Weight Operating conditions Delrin Mounts on 3 8 or 1 2 ring stand PTFE 12 VDC 0 25 A to maintain pinch 2 4 meter 8 ft connecting cable terminated with quick disconnects on valve end and 15 pin male D type connector on controller end PTFE 1 8 OD x 1 16 ID 60 cc capacity syringes Delrin Holds six syringes with thumb screws for each reservoir Manual Off or External 5 V TTL compatible BNC Connector Logic level pulse 500 ms nominal rear panel BNC connector 2 1 4 1 or 6 1 nominal dead space 110 130 or 200 250 VAC single phase 50 60 Hz 20 watt 10 40 C 50 104 F 89 x 203 x 305 mm 3 5 x 8 0 x 12 in 3 7 kg 8 Ib 4 6 kg 10 Ib Equipment is intended to be operated in a controlled laboratory environment Temperature 0 40 c Altitude sea level to 2000 m Relative humidity 0 95 Warner Inst
10. ruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 Certifications Declaration of Conformity CE MARKING EMC Application of Council Directive 89 336 EEC Standards To Which Conformity Is Declared Manufacturer s Name Manufacturer s Address Equipment Description Equipment Class Model Numbers EN55022 Class A EN61000 3 2 EN61000 3 3 EN50082 1 1992 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Warner Instruments LLC 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Valve Controller ITE Class A VC 6 Controller I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut USA Cb Gp Full Name Burton J Warner Signature Position President Warner Instruments A Harvard Apparatus Company VC 6T Manual Rev 050816 1 Declaration of Conformity CE MARKING LVD Application of Council Directive 73 23 EEC Standards To Which Conformity Is Declared Manufacturer s Name Manufacturer s Address Equipment Description Equipment Class Model Numbers EN61010 1 1993 Warner Instruments LLC 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Valve Controller Safety requirements for electrical equipment for measurement and laboratory use Class I VC 6 Controller I the undersigned hereby declare that the equ
11. s fall into either Category 8 or 9 and are currently exempt from the RoHS Directive Do Not Dispose Product with Harvard Apparatus will continue to monitor the application of the RoHS Municipal Waste Directive to its products and will comply with any changes as they apply Special Collection Disposal Required Warner Instruments A Harvard Apparatus Company
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