User Manual - Sage Science
Contents
1. Group status field will display Calibrating Calibrate G alibrate Group button eget Gol arty 1 7 ae A Pee Core fhe beteari Ih Mate C Over fon Oetertor MeF H If the calibration has been successful the Group Status field will display the message idle cal If the calibration has failed the message will revert to idle Pippin Link v 1 01 Group Status will display Idle cal sage science 12 978 922 1832 www sagescience com support sagescience com 8 Open the instrument lids and remove the optical calibration fixtures 9 Inspect and prepare the gel cassettes to be run on each instrument as outlined in the Pippin Prep and BluePippin Operations manuals 10 Place the gel cassettes into the instruments to be run 11 Close all lids 12 Press the Test Continuity button The group status window will show the message continuity during the process Group status field will Test Continuity display continuity button op Ogre on Fn ba A ek ee 00 F012 istreg duete 100212 Garter test 1 If the continuity test has been successful the Group Status field will display the message idle cal If the calibration has failed the message will revert to idle cal Pippin Link v 1 01 Group Status will display ready 13 978 922 1832 www sagescience com2. the lids on each instrument in the group ccceeeccccssccceeesececeesececeeescceeeuseceseuaeceeseeeeeeees 11 7 Press the Calibrate DUTO gasicucccnceonvecasaensaers En EaR A EEEE 12 8 Open the instrument lids and remove the optical calibration 6 13 9 inspect and prepare TNE gel 0 aE E NEEE Ea 13 10 Place the gel cassettes into the instruments tO be 13 11 VSS E o e E E E E E E E A EE A EE E ET E 13 12 Pressthe Test Continuity DUTTON 8 13 13 Open the lids TO all 00 00 E 14 14 LOA samples and or Markers coudsvsainsaunctencadnintoseans daixisdaacuinnuedhantdutbiaseasiinveigadlinrsaneimennousatedausiiares 14 15 Close the lids to all the inNStrUMents neisisissserisiscsrsireesesiirereronissessiii niesi erer innesti ir ninen 14 16 Pros aaan GOUD a mE ne Une er tan a en RE Oe en E ere er cee een 14 sage science 2 978 922 1832 www sagescience com support sagescience com Introduction Thank you for purchasing the Pippin Link control bundle Pippin Link includes all items required for the operation of group of Pippin Prep or BluePippin instruments from a single laptop computer The Pippin Link will operate 1 to 7 units simultaneously Specially modified Link equipped Pippin instruments must be ordered Please note the following important considerations e The Pippin Link control bundle and Pippin instruments will arrive preconfigured and user installable e Pippin Prep and BluePippin instruments ca
3. User Manual LINK Control bundle for multiple Pippin Prep or BluePippin instruments Version 1 01 Part No LNK 0001 978 922 1832 www sagescience com support sagescience com Sage Science Inc Suite 3150 500 Cummings Center Beverly MA 01915 2012 Sage Science Inc All rights reserved Sage Science Pippin Prep BluePippin and Pippin Link are trademarks of Sage Science Inc All other brands and name mentioned herein are property of their owners sage science 978 922 1832 www sagescience com support sagescience com Table of Contents SNE 3 TUNO KINE E TE 4 5 6 2 6 0 THEM IN SCOE 0 6 AS LAAN E OS E E E A IE EEE E E E E EN 7 TheM trunen Inter ICE sea 8 4 Using the Pippin Link Control Interface cc cccccccssecccceseccecesececeeececceeecceeseseceseuneceeseneceesensecetsgneess 9 L Select the master instrument sssssssssreresssssrsrrrresssssereresssssssreresessssettereesssssetrreeessssereresesssseene 9 2 Select the group 6 9 3 Select the protocol to be shared by the group eessssssssssessesesrerssrreessrrrssrreessrrressreresreressreresreresene 10 4 Press the Share Protocol button sssessessssssereressssssrreressssssssrrreessssserrreessssserrreressseeeerreessseeere 11 5 Place the calibration fixture on the Optical nests cccceseccccsssccecesseceeeesececceececeeeeeeessuseeetseneees 11 6 Close
4. cience com support sagescience com The Instrument Interface Instrument Tab will provide access to the local instrument interface Refer to the Pippin Prep or BluePippin operations manual for detailed instructions on monitoring programming and analysis of Pippin DNA size selection save science CALSIRATE ENAP DOT mo EHTONA Note In the instrument name may be edited in the System Options tab in the Name field The default name is the instrument serial number The name that appears in this field will appear in the Tab heading in the Pippin Link interface sage science 978 922 1832 www sagescience com support sagescience com 4 Using the Pippin Link Control Interface 1 Select the master instrument One Pippin in the group of instruments must be selected a master The protocols that are stored on the master instrument will be used to operate the group 2 Select the group instruments This should include all instruments to be run as a group including the master Select master and group instruments TEE a ee Pippin Link v LO Instrument 1 is the master Instruments 1 and 2 sage science a 9 978 922 1832 www sagescience com support sagescience com When the master instrument is selected the protocols stored on the master instrument will be displayed in the Protocols With new instruments this field will be empty Refer to the Pippin Prep or BluePippin O
5. nnot be operated within the same group due to the incompatibility of cassette definitions e Installing additional instruments that are not link equipped to a Pippin Link group requires hardware and software modifications to the instrument e Removing instruments from a Pippin Link group for the purpose of stand alone use will required the installation of a monitor and keyboard and mouse These items are not included with each link equipped instrument purchases However a monitor keyboard and mouse are included with the Pippin Link control bundle sage science 3 978 922 1832 www sagescience com support sagescience com 1 Unpacking The Pippin Control Bundle LNK 0001 will arrive with the following components e Windows based laptop computer with Pippin Link software installed The application icon will appear on the desktop e 8 Port Ethernet Hub Switch Netgear FS108 or similar e ethernet cable to connect the laptop to the hub e Pippin Link user manual this document e For stand alone Pippin operation not needed for Pippin Link operation o Computer monitor 0 Keyboard mouse and mouse pad Pippin Link Controller Pippin Link Desktop Icon Pippin link equipped instruments will arrive PIP O002 or BLU0002 with the following components e Pippin Prep L or BluePippin L instruments e Pippin Prep or BluePippin user manual e Dummy VGA monitor connector e Instrument Power Supply e Ethernet cable connects ins
6. orts on the back panel of the instruments to the Ethernet ports on the Ethernet Hub The cables do not need to be connected in any order into the hub The instruments will be auto detected by the Pippin Link software according to IP addresses that have been preset prior to shipment 9 With an Ethernet cable connect the laptop to the Ethernet Hub 10 Launch the Pippin Link Application on the laptop PC sage science 978 922 1832 www sagescience com support sagescience com 3 Software Overview The Main Screen When the Pippin Link application has been started the following screen will launch The Main screen displays the status of every Pippin in the group The adjacent tabs if pressed will display the software interface in the individual Pippin instruments in the group Group Status Group Commands sage science 6 978 922 1832 www sagescience com support sagescience com Instrument Tabs The Pippin instrument software interface is accessed by pressing The Tabs on the main screen If an instrument in connected to the group the instrument s serial number will appear in the Tab heading When an instrument is connected to the Pippin Link the Group Status window will display the message Idle 0 Pippin Link v 1 01 Instrument Serial Number appears in Tab heading Pippin Link v 1 01 Group Status will display Idle sage science 7 978 922 1832 www sages
7. perations Manual for instructions on programming protocols The cassette definitions are shown in the Cassettes field These are default definitions and must match the gel cassette product being used Master run Cassette protocols list Definitions E 077 CG m 3 Select the protocol to be shared by the group The protocol will be highlighted in blue The associated cassette definition will automatically highlight sage science 10 www sagescience com support sagescience com 978 922 1832 field in the group 4 Press the Share Protocol button The will load the protocol on all instruments in the group The selected protocol will appear in the associated Protocol Share Protocol status area butto Share Protocol appears in status area t ane lege ah Mae bt A Pee Chee fle Otei VS Mater 1 Meter 2 Dh Meow C Berd Cpr oy Ih Marte C Over fon gt ate H 5 Place the calibration fixture on the optical nests of each instrument in the group The dark side of the fixture should face down and completely cover the optical detectors Proper placement of the calibration fixture 6 Close the lids on each instrument in the group sage science 11 978 922 1832 www sagescience com support sagescience com 7 Press the Calibrate button The group status window will show the message Calibrating during the process
8. support sagescience com 13 Open the lids to all instruments 14 Load samples and or markers at outlined in the Pippin Prep or BluePippin operations manuals 15 Close the lids to all the instruments 16 Press Start Group Start Group button o me tae eget gt ou rA A eek ee ee Maree ITN Morie 5 it Oe fare Drier INF Meter 2 Mens C Berd Opare oy 1 Ih Mai C Over Soa Oetertar MeF H 17 The group may be paused restarted or aborted 14
9. trument to hub e Calibration fixture e Rinse Cassette e International plug adapter sage science 978 922 1832 www sagescience com support sagescience com 2 Set up Set up of the Pippin Link control bundle requires connecting the instruments and laptop to the Ethernet hub Addresses are set in the software application and the connections to not need to be made ina sequential order However it is recommended though not essential that all devices are completely powered up the Pippin instruments should powered and the software launched as outlined in the instrument user manual Use the following guidelines 1 Connect the dummy VGA connectors to the video monitor ports found on the back panel of the Pippin instruments 2 Connect the Pippin power supplies to the back panel of the Pippin instruments 3 Note the serial number on the back panel of the instrument Place the Pippin Instruments on the lab bench in ascending order by serial number In the software the lowest serial number will map to Tab 1 etc 4 Plug the Pippin power supplies into electrical outlets 5 Power up each Pippin instrument by pressing the power buttons in the rear of the instruments 6 Connect the power supply for the Ethernet Hub and plug in the Ethernet power supply into an electrical outlet 7 Connect the power supply for the laptop computer and power up the computer 8 With Ethernet cables connect the Pippin instruments from the Ethernet p
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