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TProfessional TRIO user manual

1. eee een 87 14 EU Konformit tserkl rung EU Declaration of Conformity 88 19 CITATI UL TIT 89 oe I Ee lols TT 90 Instruction Manual TProfessional TRIO 12 2011 5 Biometra An Analytik Jena Company 1 Introduction 1 1 Field of Applications The Biometra TProfessional TRIO is a Thermocycler licensed for PCR applications research use only and intended to be used for performing polymerase chain reactions The instrument continues the Biometra line of triple block Thermocyclers and combines the intuitive user software of the TProfessional family with a completely new hardware structure that delivers highest performance Due to an improved air stream design the exhaust is now located at the rear and higher temperature uniformity and heating and cooling rates are achieved For easy administration the TProfessional TRIO offers versatile USB functions The result is a high end easy to use Thermocycler with excellent technical specifications 1 2 Special features 1 2 1 Three independent Thermocyclers in one instrument The TProfessional TRIO Thermocycler offers three independent blocks in one housing thus different independent protocols can be run at the same time Moreover by the new temperature optimisation step TOS that makes use of the multiblock function of the instrument program steps delivering three
2. 45 5 6 3 View program prior to start 46 5 6 4 Display during operator MR 47 5 6 5 PAUSE Program CNG T R sa ee ee 49 5 6 6 COMINUS Progra sense nenn 50 5 6 7 SKIP Programi SIED nn TET 50 of SOP EENEG 52 5 7 1 Stop program from a PAUSE an nern 52 5 8 USB TUN LIONS see ee Dee are 53 5 8 1 Connect USB drive to Themocvcler nennen 53 5 8 2 Access EUSEBIO ee eine ren ee innere 53 5 8 3 Delete program in folder USB drive 55 5 8 4 Copy program from USB drive nnaannannnnnnnnnnnnnennnnnnnnnninnrrsnrnnrnnrrnnrrnnrnnrnnrrenrenne 55 5 8 9 CODY Program to USB Oliv rennen ae 56 5 8 6 Maximum number of programs on the USB drive annonnnannnnnnennnnnnnnnnnnnnnnnnnnnenne 57 SyS OMS ind ele EE 59 6 1 Wise COM AUTAU ON nee ee EE 59 6 1 1 Ci el e E 59 6 1 2 Change individual DIN 60 6 1 3 Delete account 60 6 2 Sy SUSI CON OU AO D 61 6 2 1 GONNQULE DEGI E TO tee nena me Em 61 6 2 2 Configure display contrast 61 6 2 3 Set time and date MN NM 62 6 2 4 Defragment Herb Ey E 62 6 2 5 wie QI a cci o See ae ee E 63 6 3 es le DEE 63 6 3 1 SYS RIO NE EE ODE 64 6 3 2 View run log files of the last nine runs nnnnnnannnnnnannennnnnnennnnnnnnnnnnnrrnnrennennnnne 64 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 6 4 elc 66 6 4 1 View log files of Power on self Test 66 6 4 2 Execute block extended self test 66 6 4 3 View log files from extended self test 67 6 4 4 VIEW EE 69 6
3. Instruction Manual TProfessional TRIO 12 2011 59 Biometra An Analytik Jena Company User TI 31 05 10 14 02 11 Set Language Set language for TU Deutsch Language Language Select a language using the cursor keys and press Select Language The Default Language is English Note The language for the boot process before a user is logged in can be set by the supervisor see chapter 6 6 4 6 1 2 Change individual PIN The option allows changing the PIN of the current user 6 1 3 Delete account Any user can delete his or her personal account User TIJ 31 05 10 14 10 1 System Configuration User select topic 1 1 Set language Delete account of Testuser TU Warning All programs in user directory TJ will be deleted CANCEL Press OK to confirm the account to be deleted Important To delete a user account will erase the user directory and all programs stored within Therefore prior to deleting an account ensure that the user directory is empty or save a copy of the programs to a different user directory for further usage see section 5 4 3 Note Deleted user accounts and programs can not be restored 60 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 6 2 System configuration The options in this menu allow to the general system settings 31 059 10 2 System Configuration select topic 2 1 Configure beep 27 Configure contrast 2 3 Configure time dat
4. Block Serial Na TC3860 1 Blocktype Combi 3548 Check Memory OK Communication OK LEDs OK Number af Blacks 3 Black check Sw 0360 0 02 0 02 0 32 PM 2 0O 0 0 0 5 0 0 0 5 0 0 Sensor Test Start A log file of the power on self test is stored in the Thermocycler memory see section 6 4 1 Instruction Manual TProfessional TRIO 12 2011 15 Biometra An Analytik Jena Company 4 Operating elements 4 1 The TProfessional TRIO Thermocycler front view Lid Pressure Adjustment Wheel Cover lock button Start Stop Button Display USB Port 16 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 4 2 The TProfessional TRIO Thermocycler rear view T t e BC ae 1 af T Bl fo m oT ae ined Li f j USB B Power Fuse Power Port Connection Holder Switch 4 3 The TProfessional TRIO control panel Menu Buttons LEDs Block de m Block 2 Block 3 Status l S Biometra Loginfout System Block Program amp Echt ram TU 01 test 2607 11 Preheat mE ON MT Block type Combi 3548 GEN Display Stat 7 UP zd zm 37 e Stop o Back Soft Keys Navigation Instruction Manual TProfessional TRIO 12 201 1 17 Biometra An Analytik Jena Company 4 4 The High Performance Smart Lid HPSL To achieve optimum pressure on the tubes the TProfessional TRIO is equipped with a height adjustable heated lid Close the
5. Check regulation OK Check gradient Ok Check heating performance OK Check cooling performance OK results Logfiles Press More results to display additional results from the extended block self test User TU 71 05 10 4 3 View logfile of extended block selftest Total runtime block 10 days 5 hours Number of abortions 4 Stress counter Device 1 000 010 777 Stress counter Block 1 257 000 000 Last selftest at stress counter block approximate 1 000 000 List Logfiles Press List Logfiles to go back to the list of extended block self test log files 68 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 6 4 4 View Error log files A history of all software and hardware errors is shown in this menu User TI 31 05 10 4 4 View last error logfiles L ate Time Errarcade 20 12 05 08 58 76564 CHUR 18 12 05 08 58 754 CHUP 18 12 05 08 58 754 CHUP 17 12 05 08 58 754 CHUP 15 12 05 08 58 54 CHUR 15 12 05 08 585 704 CHUR 14 12 05 08 58 54 CHUR 15 12 05 05 35 704 CHUR 12 12 05 06 55 754 CHUR 11 12 05 08 585 754 CHUR C very pu Note Several errors are displayed as message screens by the software including buttons for user interaction 6 4 5 Create service info file for the Biometra Service Department This option creates a service info file containing technical details for remote failure diagnosis by the Biometra Service Department Connect the Thermocycl
6. Open Black Pragram Directory Press New Program or choose existing program and press Edit Program to enter the program screen ee ae Deets Block Program Program Use the cursor keys to navigate in the program screen In each case the activated field is displayed inverted Corresponding to the activated field the assignment of the softkeys in the TProfessional TRIO control panel will change accordingly Note In the directory Biometra Shared protocol templates for different PCR applications are pre installed In chapter 5 3 1 instructions are given how to use these protocols The TProfessional TRIO programming spreadsheet Status view Status view Preheat Lid TU 1 08 11 Block type Edit boor am TU 01 test 26 07 11 Preheat Lid eis 939 C Block type Combi 3548 de a m s goto loops 950 0500 Defined _ 2 BEI joa cycles E 55 065 0 00 30 options Options tab 720 05 00 160 Pause Programming spreadsheet 2 To switch lid preheating on or off activate the corresponding status display and press Preheat ON OFF Preheat GN Edit Edit Save ON OFF TempOnpti Graph Save As Set lid temperature in status display C within the range of 30 C to 99 C 2 Enter temperature C and time m s in the programming table for each single step To hold a temperature for
7. User BS 3 1 View system info Hardware User BS 3 1 View system info Cyclertype TProfessional T3000 Company Biometa Serial No 2561454 Revisions No PB Software Yers PB Software Yers PB Logik Revisions No PR GSoftware Yers PR Blocktype 3 x Combi 3548 Serial No 17411519 Revisions No IO GSoftware Yersion 0350 1 33 1 15 1 20 Software Yers DD Protocol Yersion 1 0 1 0 0 4 1 0 02 1 0 WW very un info By the function Hardware Info the software versions of several hardware components are displayed Press System Info or Hardware Info to toggle between the two windows or press Overview to go back to the system main menu C Very je wy 6 3 2 View run log files of the last nine runs During each run a log file is stored in the Thermocycler memory The log files of the last nine runs can be viewed in this window Choose a program using from the list the cursor keys then press View Run Logfile 64 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company User BS 29 07 11 12 05 3 2 Select one run logfile to view Date Time User Blk Program 29 10 06 10 07 SME 1 BBE 01 schering ibc 05 10 05 08 07 SME 1 BBE 01 schering ibc 05 09 05 08 07 I 1 BBE 01 schering ibc 16 08 05 08 07 SE 1 BBE 1 schering ibc 2207 11 08 07 VW Zi OU Test 1 Run Logfile In the run log file several information for the performed run are stored For an ove
8. 34 Les emp a ES Graph Save As Instruction Manual TProfessional TRIO 12 2011 29 Biometra An Analytik Jena Company The first available program number is automatically pre selected Use cursor keys or enter two digits to move to a different program store To enter a program name press button Edit Name User TU 29 05 10 Save program Select program number LLL TU EU Testuser 1 Programs no name O2 free 03 free O4 free 05 free 06 free 08 49 Save program Select program number Lj 01 Enter program name Maximum 15 letters test CANCEL Confirm program store number with softkey Save 30 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company Save program Select program number li rw Tm 04 free 05 free 06 free Program number name and date are shown in the file directory To run the program press menu button Choose Block 19 01 Select program name or enter program number Han lii rw m 26 05 10 02 free 03 free 04 free 05 free 06 free Edit Program Select program enter no block Pee 2 free 03 free 04 free 05 free 06 free Edit Program The selected block is shown in the display Instruction Manual TProfessional TRIO 12 201 1 31 Biometra An Analytik Jena Company User TIJ Block 1 selected Block type Combi 3548 Latest programs edited r
9. 3548 Block type Combi 2548 CLERC Em 85 Temp Opti Save As To change temperature and time settings move cursor to the respective field and enter new values with the key pad Once the desired change has been made the temperature graph is updated 5 0 Ion 05 00 ao 30 50 oso 1 950 0030 72 0 B5 04650 00 0 kon 5 0 foo 30 05 00 ea quen g duni 65 0 en ENS TI te Temponpt Note The steps are automatically spread over the whole range to give maximum resolution thus steps height does not represent the real temperature Note Loops can only be set the spreadsheet mode press Edit Table Note To insert or delete program steps go to the spreadsheet mode press Edit Table Note To enter a temperature optimisation step move cursor to the respective step and press Edit Temp Opti Enter an annealing temperature and an increment as described in section 5 3 10 5 5 Change program options For each step additional options can be defined To change program options move the cursor to the right side of the spreadsheet labelled with Options An expansion of the table is displayed 40 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company User TU 26 07 User TU 26 07 Edit program TU 01 test Gees Edit program TU 01 test 26 07 11 Preheat Lid OM 99 c Block type combi 3548 Preheat lid OM 99 c Block type combi 3548 l SE m s esp Wi CONTE NC 72
10. 4 5 Create service info file for the Biometra Service Department 69 6 5 MOW TO CONLACT e ue nern ern 69 6 6 Supervisor system Confguraton nenn nnns 70 6 6 1 Change user PIN este ee ra ee ee een 70 6 6 2 Delete USER ACC OI EE 71 6 6 3 Change supervisor PUN E 71 6 6 4 Sel DOO AIGA E 72 6 6 5 Delete all user accounts nennen 72 7 Short VAN ET met 74 8 Ebert 79 8 1 Slow heating and COMING EE 79 8 2 AUO E EE 19 8 3 Restart due to unrecognised power falure nennen 79 8 4 Adaptation of protocols from other cyclers cccccceccceecceeceeeeeeeceeeeeeeteeteeteeeteeeanes 79 8 9 Releasing wheel in case of blocked lid 2002200220002200nnne nenn nenn nenn nennen 79 9 Maintenance and Re EEN 81 9 1 Cleaning and Maintenance nenn nenn nenn nenn nnnennnennnnnn san snnnnnnn nennen 81 9 2 Servicing and repair 81 9 3 Slgu VI Ei ee E ee ee ne een 81 9 4 Replacement of spare parts sssssssessssssssseeeeeen nennen nennen nsns nsns nennen 81 UMS eI een 82 1O Idem 82 m SEVICE tc eeee 83 11 1 Instructions for return shupment san nnnennnennnennne nenn nnnennnennnennnenn 83 11 2 Packing of the Thermocycler un n naar 84 12 Equipment Decontamination Certificate ccccccceeceeeceeeeeneeeneeneecnesenesenesenesenenenenanes 85 13 Note for the disposal of electric electronic waste
11. Enter or step to the next field with the right cursor key 5 3 5 Enter step temperature and time lt Use cursor keys to navigate within the programming table Note Each setting is confirmed with Enter The cursor moves automatically to the next field Alternatively you can confirm a value by moving forward with the cursor keys Instruction Manual TProfessional TRIO 12 201 1 27 Biometra An Analytik Jena Company User TU 11 Edit program TU 01 test 25 07 11 Preheat Lid OM 99 C Block type combi 3548 sa Jsat 1 950 foa so mal zo BE tT E Enter temperature for the first step and press Enter In the next column you can enter the time for this temperature Note Minutes and seconds can be separated by dot but this is optional You can also enter the digits one after the other Example To set 2 minutes 30 seconds enter 2 3 0 Subsequently 2 30 m s will be displayed in the programming spreadsheet Note For Biometra Thermocyclers a dot can be used as a shortcut to program minutes H e H Example To set 2 minutes enter 2 e Subsequently 2 00 m s will be displayed in the programming table 5 3 6 Set loop Note In general loops are entered in the LAST STEP of the loop A loop is defined by selecting the FIRST STEP in the loop plus the number of back loops Enter the number of the first step within the loop in the column goto and confirm
12. Nee onen 20 5 1 4 BIOCK TOT ea a um 20 5 1 5 SV Ste PN VI Alec een E ae ee 21 5 1 6 POG Fala ME 21 ef TProressional TELE ed ER 5 2 1 Beie BI MCCC KT 22 0 2 2 Create new user aCCOount seen snn nnne nena nenn sanas nsa nennen 24 5 2 3 SUDEWISOF LOG IN ers 24 5 2 4 Ree BEE 25 3 9 Create Drogral E 25 9 9 1 Create program from Template 25 Instruction Manual TProfessional TRIO 12 201 1 3 Biometra An Analytik Jena Company 0 90 2 Create program without template nenn nenn nennen 26 5 3 3 Set lid pre heating mode nano nnnnennne nenn nenne nenn nnne nnns 26 5 3 4 SEE IC Tempera lUl apanr SEI QercePias p apyc DI SE TUE TIMM BUS IUPSM D ERES EETUEEU i 27 9 3 5 Enter step temperature and me 27 5 3 6 lee ne a a Se Ener lee 28 5 3 7 Cool below ambient temperature nennen nnnnen 29 5 3 8 Program PAUSE ee ee TETTE 29 5 3 9 SAVE Progi Al einen UT 29 5 3 10 Set temperature optimisation sien 32 5 4 E Ol PFOGFAMIS RENE TE 34 5 4 1 insert EOC SUC EE 34 5 4 2 Dlete programi SEC E 36 5 4 3 CODY ONO AN E OE T 37 5 4 4 OTST NOG aD T 38 5 4 5 Editing programs in the graphical mode nennen nennen 40 5 5 e Lee Regele Ten ele e een en none cence cedar ee eee 40 5 5 1 Heating and cooling rale DERE 41 5 5 2 Time increment gt Ate 42 5 9 9 Temperature increment or decrement gt ATC 42 5 6 PS OO CAN ee ee m 43 5 6 1 Select program for start from user directories nennen nennen 43 5 6 2 Quick start programs from the block menu
13. Program In the next screen press Save copy to USB ser TI 19 12 11 Copy program TU 01 test to Li C9 USB Drive 04 FC TU Testuser 3 Programs O1 test 14 10 11 02 pcr 2 25 07 11 Us rt pcr 25 07 11 4 05 free 06 free The system displays a message with the progress in the copy process 56 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company ser AA 13 12 11 Lopy program AWA 01 no name to Li USB Drive ai LC Copying program to USB Please wait Checking USB Stick Reading File List Checking memory conditions Saving Program After the copying is completed the new program is added to program list on the USB drive Note When copying programs to the USB drive changes of the name of files will not be saved If you use the function Edit name and set a new name the changes will not be saved when the file is going to be stored to the USB drive Save copy to USB Please save the program with the new name to your user directory first and then use the function Save copy to USB ser AA 19 12 11 Lopy program AWA 01 no name to LC Enter program name Maximum 15 letters USB Drive Save copy to USB Changes won t be saved CANCEL 5 8 6 Maximum number of programs on the USB drive User BB 13 12 11 17 09 Save program Edit name XI USB Drive Allowed Number af 48 files has beed exceeded Instructi
14. SUP Change user PIN Testuser Enter new DIN Repeat PIN Overy jew 6 6 2 Delete user account The supervisor can delete user accounts regardless if they are password protected or not Select the appropriate user directory from the list and press Select User User SUP 01 06 10 08 59 SUP Delete User select user to delete BS Biarmetra Shared SIR Supervisor TU Testuser Select the user account to be deleted and confirm with OK ser SUP 01 06 10 02 00 SUP Delete User select user to delete Delete account of Testuser TU 7 Warning All programs in user directory TL will be deleted CANCEL The directory and all programs therein are deleted 6 6 3 Change supervisor PIN Instruction Manual TProfessional TRIO 12 201 1 T1 Biometra An Analytik Jena Company The supervisor account is protected by a PIN code The default Supervisor PIN is 000 000 0 do not enter blanks Enter your new PIN and repeat your input Confirm with PIN OK User SUP 01 08 11 SUP Change SUP PIN Supervisor SUP I Attention Forgotten SUP PIM can only be changed by your Distibutor Enter current PIN Enter new PIN Repeat PIN Note Please make a note of the supervisor pin und keep it on a safe spot A forgotten supervisor PIN can only be reset by the Biometra Service Department or authorized Biometra distributors 6 6 4 Set boot language The language displayed during the boot process can be set by the sup
15. d chets des appareils lectrique lectronique EE This symbol the crossed out wheelie bin means that this product should be brought to the Renseignement return and or separate systems available to end users according to yours country regulations when this product has reached the end of its lifetime For details please contact your local distributor This symbol applies only to the countries within the EEA EEA European Economics Area comprising all EU members plus Norway Iceland and Liechtenstein Dieses Symbol die durchgestrichene Abfalltonne bedeutet dass dieses Produkt von der Firma Biometra f r eine kostenlose Entsorgung zur ckgenommen wird Dies gilt nur f r Ger te die innerhalb Deutschlands gekauft worden sind Kontaktieren Sie f r die Entsorgung bitte die Biometra Service Abteilung Au erhalb Deutschlands wenden Sie sich bitte an den lokalen H ndler Dieses Symbol gilt nur in Staaten des EWR EWR Europ ischer Wirtschaftsraum umfasst die EU Mitgliedsstaaten sowie Norwegen Island und Liechtenstein Cet symbol conteneur d chets barr d une croix signifie que le produit en fin de vie doit tre retourn un des systemes de collecte mis la disposition des utilisateurs finaux en cons quence des r gulations par la loi de votre pays Pour des information additionel nous Vous demandons de contacter votre distributeur Cet symbole s pplique uniquement aux pays de I EEE EEE E
16. lid After the samples have been placed in the block close the lid Turn the wheel clockwise until you hear a clicking noise In this mode the pressure will not increase further even when you keep on turning the wheel ro nmo aoU eoooooo Qr O OO OO O O Opm OOOOO OO Ow OOOOOOOOsR OODOOOO Ola E COD OO OO Oe Note The pressure of the lid has been optimized for a fully loaded block If only very few tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure Open the heated lid First Release pressure by turning the wheel counter clockwise As soon as there is no more resistance the pressure has been released Then Now you can open the lid with the knob Important The lid should not be opened under pressure because this leads to damage of the locking mechanism 18 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 5 Operating 5 1 The TProfessional TRIO user interface The TProfessional TRIO user interface provides four menu buttons above the screen and four softkeys below the screen Menu buttons Login out System Block Program User 27 05 10 11 38 To log in select user or enter first letter SUP SUP Supervisor TU Testuser Ex E almae OC I Softkeys Ga 5 1 1 TProfessional TRIO menu buttons The four menu buttons allow quick access to the TProfessional TRIO main menus Thes
17. modified by unauthorized personal Note Your PIN can be changed any time in the System menu see section 6 6 1 5 2 3 Supervisor Log in Only the supervisor is authorized to change global instrument settings This includes update of firmware and the deletion of user accounts see section 6 6 24 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company To log in as supervisor press the Log in out menu button above the display Press softkey Supervisor Log in enter supervisor PIN and confirm with PIN code OK The default supervisor PIN is 000 000 0 do not enter blanks 5 2 4 Delete User Once logged in you can delete your own account in the System menu see section 6 1 3 In addition any user can be deleted by the supervisor To delete a user log in as supervisor see section 6 6 2 and select menu option delete user Choose the user account you would like to delete and confirm with OK Note By deleting a user account all programs in the referring user directory will be deleted It is therefore recommended to safe the programs in a different directory prior to deleting a user account 5 3 Create program At Thermocyclers of the TProfessional series programs can be created in two ways Pre installed program examples may be used see chapter 5 3 1 or new programs without a template created see chapter 5 3 2 5 3 1 Create program from template Log in to your personal user directory a
18. provides a maximum of convenience to the customer 1 2 5 USB port By using an USB stick protocols can be exchanged between instruments Therefore by the USB functions it is very easy to synchronize the memory contents of instruments Instruction Manual TProfessional TRIO 12 2011 T Biometra An Analytik Jena Company 1 3 Technical specifications TProfessional TRIO Thermocycler Order number 070 720 070 723 070 724 TProfessional TRIO 30 TProfessional TRIO 48 TProfessional TRIO combi Capacity 3 blocks for 30 x 0 5ml 3 blocks for 48 x 0 2ml 3 combi blocks for 18 x 0 5ml tubes each tubes tubes or 48 well microplates or 48 x 0 2ml tubes or 6 x strips of 8 each or 48 well microplates or 6 x strips of 8 0 6 C at 95 C 0 3 C at 70 C 0 2 C at 55 C 3 C to 99 C Temperature Uniformity Temperature range Multiblock tool Temperature Optimisation Step TOS Toggle between easy spreadsheet and graphical mode adjustable ramp rates time and temperature increments extended self test service info files USB functions Program memory 30 individual subdirectories 350 average programs Display Ya VGA screen 320 x 240 pixel Auto restart after es power failure Cool samples at Y 4 C Y Heated lid High Precision Smart Lid for optimal lid pressure and excellent temperature uniformity Lid temperature ee range en 5 35 C 70 relative humidity Working conditions According t
19. side of the control panel see also chapter 4 3 to start the program on the selected blocks Note To start programs containing a temperature optimisation step programs with extension TOS all three blocks are required Therefore please ensure that none of the blocks is currently in use see chapter 5 3 10 5 6 3 View program prior to start To check the program prior to start press softkey View Program User TIJ 26 07 11 Block 1 2 3 selected Block type Combi 3548 Latest programs edited run by TU 1 TU 2 TU 402 per 2 26 07 11 Ec Multiblock View Block 1 3 Selection Praaram The current program the estimated run time and the selected blocks are shown 46 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company ser TU 5 07 View program TU 01 test 25 07 11 All SOCKS Sel Preheatlid ON 99 C KIELER 550650030 72 0 Joao 2 34 Options zo sol 160 Pase Run Time 1h 18m Latest Program List To display the temperature optimisation step settings use the cursor keys to select the corresponding program step and press View TempOpti To toggle between the graphical and spreadsheet mode use the softkeys View Table and View Graph Spreadsheet mode Table Graphical mode Graph User TI 6 07 Fila User TU 26 07 11 View program TU 01 test 26 07 11 View program TU 01 test 25 07 11 All SOCKS Sel Preheatlid ON 99 C
20. the Stop button on the control panel see chapter 4 3 User TU 71 05 10 Select block with cursor right left or softkeys Block 1 Ste Block 3 active active free TU TU Ue pcr z 03 rt pcr Testuser TU Testuser TU 1h4 m h 55m 15 24 14 32 Block 1 Block 2 all blocks To stop a program step press the corresponding softkey Stop Block 1 Stop Block 2 Stop Block 3 or press softkey Stop all blocks User TU 31 05 10 Select block with cursor right left or softkeys Block 1 Ste Block 3 Stop active program on block 1 27 CANCEL Confirm with OK 5 7 1 Stop program from a pause To stop a paused program press the Stop button on the control panel see chapter 4 3 and proceed as described in chapter 5 7 Note If the instrument is switched off during a pause step this will be recognized as a power failure A referring error message is written to the log file and the program will be continued at the paused step Therefore it is necessary to stop a paused program before the instrument is switched off 52 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 5 8 USB functions By the USB port at the front side of the instrument see chapter 4 1 programs can be transferred from or to a USB drive 5 8 1 Connect USB drive to Thermocycler Plug in the USB drive to the corresponding USB port at the front side of the instrument As long as no USB drive is connected the
21. the heat sink The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner 8 2 Autorestart Running programs will be continued from the same step where the power failure has happened as soon as the power is supplied again A message is displayed showing a that a power failure occurred during the run and b when and at which step the program was started again To log in select user or enter first letter Auto restart 05 45 01 12 06 08 in step 1 706 1 1 Confirm with OK to close the message screen 8 3 Restart due to unrecognised power failure High voltage fluctuation can lead to an automatic restart of the Thermocycler In this case the cycler restarts at the step where there power failure has occurred To avoid voltage fluctuation do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 8 4 Adaptation of protocols from other cyclers Since the TProfessional TRIO is a fast instrument it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 5 5 1 Alternatively the time settings may be extended 8 5 Releasing wheel
22. with improved air stream design and a completely new hardware environment the TProfessional TRIO achieves higher heating and cooling rates and better temperature uniformity High ramping rates provide both short experimental times and increased specificity The instrument works quietly and consumes little power which in turn leads to low heat emission and by the compact footprint it saves valuable bench space 6 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 1 2 4 Smart lid technology The TProfessional TRIO Thermocyclers heated lids prevent condensation and provide reliable contact between samples and thermoblock The lids are powered by Biometra s well known omart Lid technology with integrated clutch mechanism which automatically limits the lid pressure applied to the plasticware and thus saves tubes from damage In addition by formation of a homogeneous air cushion an even temperature distribution between the samples is ensured significantly improving temperature uniformity This also ensures reproducible conditions amongst different PCR runs The temperature of the independently working lids can be set individually in a range between 30 and 99 C This ensures optimal conditions also for other applications as PCR like e g enzymatic restriction reactions With one press on the front button the lids gently swing open and arrest in their end position In combination with the now easier to access blocks this
23. 0 0500 i60 j Pase Edit program TU 01 test 25 07 11 Preheat lid OM 99 c Block type Combi 3548 S sn 955 sa SsDacso oo eg 720 sol ei jPase Save As If options have been changed from the default values there is a as indication in the options field 5 5 1 Heating and cooling rate Since the TProfessional TRIO is a very fast thermocycler it may be necessary to reduce the heating and cooling ramp to adopt protocols from slower thermocyclers The average heating or cooling rates can be set in the column labelled with A C s Instruction Manual TProfessional TRIO 12 201 1 41 Biometra An Analytik Jena Company User TUI JP Edit program TU 01 test 25 07 11 Preheat lid OM 99 c Block type combi 3548 K m s Kies 1 ff 4 0 RR REES RER 4a NE Ea AS Note This entry specifies at which step the set speed is achieved In example to heat from step 2 to step 3 with the desired speed the heating rate in step 3 has to be lowered Note To reduce the over all speed of the Thermocycler the heating rate has to be modified in all steps The speed settings will be only valid for this individual program 5 5 2 Time increment At s To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle Enter the desired time increment seconds in the column labelled with dt s This value will
24. 550 000 options 720 pem 369 Pase Insert 26 07 Edit program TU 01 test 25 07 11 Preheat Lid OM 99 C Block type Combi 3548 Insert step before step no 4 Edit program TU 01 test 25 07 11 Preheat lid OM 99 c Block type Combi 3548 S sn 955 Sal 50450 RR EEG 720 sol Note The new step has been entered within an existing loop This loop now consists of four steps Instruction Manual TProfessional TRIO 12 201 1 35 Biometra An Analytik Jena Company 5 4 2 Delete program step Move cursor to the step that should be deleted and press softkey Insert Delete Step User TIJ 20 07 Edit program TU 01 test 26 07 11 Preheat lid OM 99 c Block type combi 3548 S sn 955 Sal 50450 RR oo 720 sol Edit program TU 01 test 25 07 11 Preheat Lid OM 99 c Block type combi 3548 s sn 955 Sal 55 Da650 00 30 100 foo 720 sol Insert Edit program TU 01 test 26 07 11 Preheat Lid OM 99 c Block type combi 3548 Delete step na 4 CANCEL 36 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company Confirm with softkey OK User TU 26 07 11 Edit program TU 01 test 25 07 11 Preheat Lid OM 99 C Block type Combi 3548 DC 950 Im S50 0030 oO 65 0 0 b Options 720 os
25. Biometra An Analytik Jena Company Professional TRIO Thermocycler Instruction Manual Ver 11 12 Model Ord No TProfessional TRIO 30 070 720 TProfessional TRIO 48 070 723 TProfessional TRIO combi 070 724 Please read these instructions carefully before using this apparatus Biometra GmbH Rudolf Wissell Str 30 Service Department D 37079 G ttingen Rudolf Wissell Strasse 14 16 Tel 49 551 50686 0 D 37079 G ttingen Fax 49 551 50686 66 Tel 49 551 50881 10 12 email info biometra com Fax 49 551 50881 11 Internet http www biometra com email service biometra com Biometra An Analytik Jena Company This document describes the state at the time of publishing It needs not necessarily agree with future versions Subject to change 2 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company Contents 1 Idee e de RT 6 1 1 Felid O1 APDIICANONS MR UL T 6 1 2 SPECIAL Tet ll OS a Ped miM TI MEE RM SEEN PEU E 6 1 2 1 Three independent Thermocyclers in one instrument nannannnnennnnnennnnnennnenne 6 1 2 2 Easy PIOGTAMMING NR RREE 4 6 1 2 3 FANN SOS CC m 6 1 2 4 SMa lee le ele KEE 7 1 2 5 Eeer 7 1 3 Technical specifications TProfessional TRIO Thermocycler 8 1 4 HS AN
26. Black 1 2 3 selected as T 95 0 pe des 35x Im test Program List m 3 72 0 I n on s0 05 00 65 0 a B5 FT H anram Lie Note In the program preview mode a program cannot be edited To edit programs press menu button Program above the display see chapter 5 1 2 5 6 4 Display during operation After at least one block has been started the following screen is shown Instruction Manual TProfessional TRIO 12 201 1 47 Biometra An Analytik Jena Company ser TIJ 31 05 10 Select block with cursor right left or softkeys Block 2 Pause Block 3 free Block 1 active TU TU Ue pcr 2 03 rt pcr Testuser TU Testuser TU 1h4 m h 55m 13 17 12 25 Overy jew Temperatur The status of blocks can be free active or paused The user that started the program and the estimated run time are shown To display detailed information for a running program press the corresponding softkey Block 1 Block 2 or Block 3 Three different display modes are available during the run Show Time Show Graph Show Table User TU 31 09 10 is Lker TU 31 09 10 Bik 1 active Ie Bik 1 active Ieee Bik 1 active Bik 1 active SM 0 User Testuser TU j Block 95 0 C Sten0l 05 95 0 C Step 01 05 Co o O Bol cp D me Steps on ms Block type Unknown Remaining Time 1h 47m 95 0 C Estimated End 13 23 B NNNM 01 00 Tz i Ba gl Mar Mid Esti
27. EN61010 1 EN61010 2 010 Dr J rgen Otte Quality Manager 88 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 15 Warranty This Biometra instrument has been carefully build inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship as described under the following conditions This warranty is valid for 24 months from date of shipment to the customer from Biometra This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the instrument is operated as described in the manual Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused Instruction Manual TProfessional TRIO 12 2011 89 16 Subject Index Autorestart ccccecc cece eeceeeeeeeeeeeeeneeenees 19 22 0 61 Block menu 20 GONE ee ee 69 Declaration of Conformity 88 Decontamination Certificate 85 Defragmentation ccccccceeceeeeeeeeeeaees 62 Display Seni 61 ED SOO SAI ee 87 Firm
28. Thermocycler will try to read the memory content and subsequently display a message No disk drive found User AWA 19 12 11 Select program OO o oo Li I use Drive m No Diskdrive found Note The maximum memory size of the USB drive should not exceed more than 2 GB 5 8 2 Access USB drive Log in to your user directory and press menu button Program see chapter 5 1 6 Instruction Manual TProfessional TRIO 12 2011 53 Biometra An Analytik Jena Company Leer BB 19 12 11 Select directory Li USB Drive G Barbara Biametra Shared Michael Testuser Program Directory Use the cursor keys UP and DOWN to select the directory USB Drive After selecting the USB drive the memory content is read automatically by the Thermocycler and the message Reading File List displayed ser AA 19 12 11 Select program Li USB Drive Reading File List After reading the memory content a list of programs available on the USB drive is displayed 54 User AMA 19 12 11 11 10 Select program HH USB Drive 01 4 Programs Awa Olnco name 19 12 11 BB O2tadimtw 19 12 11 JS Uinonarme 19 12 11 J5 _02n0 name 19 12 11 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 5 8 3 Delete program in folder USB drive To delete a program from the USB drive press Delete program see chapter 5 8 2 In the next screen confirm your selectio
29. a program from a user directory Note Programs in pin code protected user directories cannot be deleted 38 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company User TIJ 26 07 15 44 Select program name or enter program number m mu T FC TU Testuser 4 Programs 01 test 26 07 11 TOS U2 per 2 20 07 11 Us rt pcr 25 07 11 S04 demo 05 free 06 free Program Press softkey Delete Program User TU 26 07 11 Select program name or enter program number Hau HD vu o Delete program TU 04 demo 15 44 Select program name or enter program number FC TU Testuser 3 Programs 1 test 28 07 11 TOS Ue pcr 2 25 07 11 Us rt pcr 25 07 11 4 05 free 06 free Program This program store is now free Instruction Manual TProfessional TRIO 12 2011 39 Biometra An Analytik Jena Company 5 4 5 Editing programs in the graphical mode Programs are created in a programming spreadsheet where new steps are easily defined by temperature and time In addition programs can be edited in the graphical mode which provides a schematic temperature plot of the different steps To toggle between this two modes use the softkeys Edit Table or Edit Graph Spreadsheet mode Table Graphical mode Graph er TU 3 06 User TU Edit program TU 01 test 25 07 11 Edit Edit program TU 01 test 6 07 11 000 25 07 11 PreheatLid ON 99 C Block type Combi
30. an indefinite time enter 0 in column m s and press enter Subsequently the word Pause is shown in the display Note Minutes and seconds can be separated by dot or digits can be entered one after the other Example To set 2 minutes 30 seconds enter 2 e 3 0 or 2 3 0 gt To insert or delete a program step move cursor to the corresponding position in the programming table and press Insert Delete Step Instruction Manual TProfessional TRIO 12 2011 15 Biometra An Analytik Jena Company Insert Delete Edit Edit Save Sten prm Graph Save Ae 2 To insert or delete a program step press Insert Step or Delete Step The position at which a step will be inserted or deleted is highlighted CANCEL Delete Insert Step Step 2 Inthe next screen confirm insertion or deletion with OK 2 To define a loop a enter the step the program jumps go back to goto and b enter how often the loop is repeated loops The defined loop is graphically displayed by a bracket at the left side of the programming spreadsheet the total cycle number is mentioned 2 To program special features like temperature increment time increment or heating and cooling rate repeatedly press the cursor key right to open the options tab A new table is displayed Preheat Lid OM 99 C Block type Combi 3548 e Tip storing samples at 16 C rather than 4 C at the end of a run will increase the in
31. be active with a program running or free 20 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 5 1 5 System menu To enter the System menu press the menu button System above the display User TI 27 05 10 Topic overview select topic or enter topic no 1 User Configuration change language change PIN delete user System Configuration beep contrast set date time defragment memory screen saver System Info run logfile serial nurmber black info software versions Service selftest execute extended block selftest create Service Info File error log How to contact Biometra The System menu allows global settings and provides information about the instrument The power on self test log files can be retrieved and service files generated a detailed description of this menu is shown in section 6 5 1 6 Program menu To enter the Program menu press menu button Program above the display User TIJ 27 05 10 Select directory I SUP X Supervisor I ME Testuser Block Program Directory In the Program menu existing programs can be edited or new programs can be created Each user has its own user directory where individual programs are saved Programs from other user directories can be used or copied but not modified Instruction Manual TProfessional TRIO 12 2011 21 Biometra An Analytik Jena Company 5 2 TProfessional TRIO software The TProfessional TRIO Thermoc
32. be added to the time value from cycle to cycle Note Be sure that the time increment is set in a step that lies within a loop Otherwise there will be no iterative time increase Note A time increment will increase the total runtime depending on the numbers of cycles and the size of the increment A program with many cycles and large time increments will take a significantly longer time than a standard protocol 5 5 3 Temperature increment or decrement gt AT C For some applications it is useful to start with a higher temperature and to decrease this temperature from cycle to cycle This subsequent lowering of a temperature is called touch down 42 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company To decrease a temperature from cycle to cycle enter a negative temperature increment in the column labelled with AT C Note Be sure that the temperature decrease is set in a step that lies within a loop Otherwise there will be no iterative temperature decrease 5 6 Run program To start a program press the Start button on the control panel see chapter 4 3 This button is only active when a program has been selected for start This is indicated by the green LED in the start button 5 6 1 Select program for start from user directories Any program can be started by any user Also programs from other user directories can be started To select a program for start press the Program men
33. block and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program Do not heat samples without having the lid locked securely Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liquid may quickly spill out Do not touch the heated lid Use only suited plastic ware in the TProfessional TRIO Thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids Instruction Manual TProfessional TRIO 12 201 1 PPPPP PPP gt gt o eee EPP Biometra An Analytik Jena Company The TProfessional TRIO Thermocycler contains no user serviceable parts Do not open the instruments housing Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Do not use the instrument when damages of the housing block cable or other parts are visible Prior to connecting the unit to the power source please ensure that the voltage selector at the bottom of the instrument is set to the required voltage Make sure that the main supply voltage is in accordance with the label above the power connection see section 4 2 Unplug the power cable before you open the TProfessional TRIO Thermocycler Danger
34. block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used Ensure that both the rear and bottom ventilation slits at the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument The TProfessional TRIO Control Panel Menu Buttons LEDs Block 1 Block u Run u Pas Status Biometra e S 3 ME Display Start E o Stop Ne Back Soft Keys Navigation Log in After the TProfessional TRIO has passed the initial self diagnosis the login menu is displayed Press Log in and log in as existing user 2 Press Supervisor Log in and log in as supervisor Press New User and create new user account The language displayed in the user interface can be selected English or German The selected language will be stored for each individual user Log aut Select Language 2 Press Select Language to select preferred language To set boot language log in as supervisor and select boot language in the systems menu Press Select Language to set boot language T4 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company Create modify and save programs To create a new or edit an existing program press the Program menu button gt Move cursor to your directory and press Open directory Choose
35. ce is free from biologically dangerous and radioactive contaminants The declaration of decontamination see section 12 must be attached to the outside of the packaging e Use the original packing material If not available contact Biometra or your local distributor e Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT e Please enclose a note which contains the following a Senders name and address b Name of a contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible Instruction Manual TProfessional TRIO 12 2011 83 Biometra An Analytik Jena Company 11 2 Packing of the Thermocycler Biometra uses an extra designed packaging system where the instrument is mounted in between two tearproof foils The Thermocycler is put onto the lower inlet and is fixed in between the foils by pressing the upper inlet down Note The Thermocycler is only protected from transport damage if the packing instructions are followed and the instrument is mounted in between the foils Biometra will not be responsible for transport damage by improper packing Ger t zwischen den Folien schwebend einspannen Mount instrument floating in between the foils 84 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 12 Equipment Decontamination Certificate To enable us to comply wi
36. ck with cursor right left or softkeys Block 1 Block 2 J Block 3 active active active TU TOpt cal TU TOpt wl TU TOpt ai ul test O1 test O1 test Testuser TU Testuser TUS Testuser TU 2h 2m Oh Om Oh Om 15 36 13 34 13 34 Overview Temperatur To pause an active program select the corresponding block and press Block 1 Block 2 or Block 3 In the next screen press Pause Block Skip Step The message Pause is shown in the display for the paused block Pause Block SES Graph Show Time Overy jE Skip Step Block 1 3 Instruction Manual TProfessional TRIO 12 2011 Tf Biometra An Analytik Jena Company To continue a paused program select the corresponding block and press Block 1 Block 2 or Block 3 In the next screen press Continue Continue SES Graph Show Time Der view Block 1 3 2 To stop an active program press the Stop button on the control panel Select a single block Stop Block 1 Stop Block 2 or Stop Block 3 or select all blocks Stop all blocks to be stopped In the next screen confirm with OK Stop Stop Stop Stop Block 1 Black z Black 3 all blacks Programs with a pause in the last step have to be stopped manually as well 78 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 8 Trouble shooting 8 1 Slow heating and cooling The TProfessional TRIO is equipped with a strong ventilator for the cooling of
37. d send back the warranty registration card This is important for you to claim full warranty 3 3 Installation conditions e Place the TProfessional TRIO Thermocycler on a stable surface in a dry safe environment For details see working conditions in table Technical specifications see chapter 1 3 e Let equilibrate the TProfessional TRIO Thermocycler to room temperature before starting operation 1 to 6h e Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains e Make sure that the ventilation slots on the bottom and the rear are not obstructed see section 4 2 Make sure that there is no object underneath the thermocycler that may block the ventilation slots at the bottom e g a piece of paper etc e There must be sufficient distance between the ventilation slots at the rear of the Thermocycler and a wall or another instrument min 10 cm Ensure that both the side and bottom ventilation slits of the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument Instruction Manual TProfessional TRIO 12 201 1 13 Biometra An Analytik Jena Company e Make sure that the main supply voltage is in accordance with the label above the power connection see section 3 4 e Connect the TProfessional TRIO Thermoc
38. different annealing temperatures can be created easily This makes the TProfessional TRIO Thermocycler the perfect instrument for laboratories with the demand for high flexibility and the need to often optimise new PCR protocols With a maximum capacity of 3 x 48 wells the TProfessional TRIO also offers high throughput in parallel operation The TProfessional TRIO Thermocycler is available in three different block versions for 0 2 ml tubes 0 5 ml tubes or both in the combi block 1 2 2 Easy programming The TProfessional TRIO Thermocycler offers the intuitive TProfessional software with easy spreadsheet programming philosophy New programs are easily created in a well arranged screen that avoids the need to toggle between different windows This makes the creation of new or editing existing programs fast and easy One touch leads from the spreadsheet to an alternative graphical programming mode Four softkeys directly below the display offer quick access to all functions needed in the individual context and four menu buttons above the display to the main menus For easy retrieval programs can be stored in 30 individual subdirectories optionally password protected By the administrator function the accumulation of unused programs or user directories can be managed to keep the memory content up to date Keyboard and display are arranged at an angle to ensure reflection free viewing and ergonomic programming 1 2 3 High speed By the elegant housing
39. e 24 Defragment memory 25 Configure screen saver Choose a single menu item using the cursor keys then press Select or press Overview to go back to the system main menu 6 2 1 Configure beep To configure the system beep use the cursor keys to set the beep on or off and confirm selection with softkey Save User TU 71 05 10 2 1 Configure beep Beep Beep ON Overy jew The signal beep sounds when a program is going into a pause or is stopped The signal beep can be stopped by pressing any key except the Stop button on the control panel The setting for Default Beep is beep off 6 2 2 Configure display contrast The display contrast can be set individually for each user Instruction Manual TProfessional TRIO 12 201 1 61 Biometra An Analytik Jena Company User TU 31 05 10 14 40 2 2 Set display contrast Change settings with the cursor keys and confirm with softkey Save Contrast The setting for Default Contrast is the median position 6 2 3 Set time and date Enter settings for date and time and confirm with softkey Set Time and Date ser TU 41 05 10 2 3 Set date and time Day Month OG Year 010 Date Hours 14 Minutes 45 and Time 6 2 4 Defragment memory By erasing and creating PCR protocols after a while new files may be stored in a fragmented fashion in the memory of the TProfessional TRIO Thermocycler This leads to increased memory access times and the soft
40. e are e Login out menu e System menu e Block menu e Program menu The active menu is indicated by a graphical link to the respective menu button Login out System Block Program 27 05 10 11 38 The example shows Program menu is active The four menu buttons are permanent and always have the same function This is in contrast to the softkeys The softkey functions change depending on the current software menu 5 1 2 TProfessional TRIO softkeys Below the display there are four softkeys Instruction Manual TProfessional TRIO 12 201 1 19 Biometra An Analytik Jena Company Logout peer wow EET T i Log in In contrast to the menu buttons the softkeys have changing functionalities which are shown in the above display line 5 1 3 Log in menu To enter the Log In menu press the menu button Log in out above the display When the instrument is switched on the log in screen is displayed To use the instrument a user has to be selected Alternatively a new user can be created 27 05 10 To log in select user or enter first letter SUP SIJP Supervisor TU Testuser FR m Log in 5 1 4 Block menu To enter the Block menu press the menu button Block above the display 27 05 10 Select block with cursor right left or softkeys Block 1 Block 2 Block 3 free free free Overview Temperatur The Block menu shows the current status of each of the three blocks Each block can
41. el Supervisor Testuser Log in Select user with cursor keys or enter first initial The cursor jumps to the first account starting with this letter 22 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 27 05 10 To log in select user or enter first letter Barbara Michael Supervisor Enter 7 digit PIN code for TU Testuser Remember to log out when this cycler is no longer under your Supervision PIN code OK Welcome Lurrent user Testuser TU To continue press one of the above menu buttons To proceed with editing or running a program select the menu button Program or Block above the display Instruction Manual TProfessional TRIO 12 2011 23 Biometra An Analytik Jena Company 5 2 2 Create new user account Press menu button Log in out above the display 12 12 02 To log in select user or enter initials Barbara Eva Frank Jonanthan Log in Register new user Enter your name initials 1 15 letters 2 3 letters Enter PIN optional Repeat PIN 1 7 digits Iser Data OK Enter your name and 2 3 initials Enter a personal identification number PIN consisting of 1 7 digits For security reasons you are requested to repeat the entry of your PIN Note PIN entry is optional If no password protection is wanted just confirm with User Data OK However if no PIN is entered you account containing all of your programs can be
42. el ER RR 9 1 4 1 PCR License Legal Disclaimer cece ceeccceeceae cece eeeeeeeceeeseueeseeessneeseeesaeeeaes 9 1 4 2 OTN RM 9 1 4 3 Bre 9 1 4 4 Meaning of the instructions nennen nennen nnns 9 2 Safety and Warning Notices uus 22002000 00002n0nannnnnnnn nun nnnnn nun nnnun nun nnnnnnnn nun nnnnnn nenn 10 2 1 Denion oro y MOOIS nee ea 10 2 2 General Safety Instructions ccccccccscccsseceeeeeeeeeeeeeceeecseesaeeseuceseesseesauessueeseeessuss 10 MEV SCANLON Met 13 3 1 COMENTO CSUV GLY anreisen 13 3 2 Unpack and ene 13 3 Installation COMMONS en ee m 13 3 4 ODEFAUOR e E Le E 14 3 5 riim m C 14 4 Operating elements Eucerin cda eee eee eue aue ee as inei ui osse 16 4 1 The TProfessional TRIO Thermocycler front view 16 4 2 The TProfessional TRIO Thermocycler rear view 17 4 3 The TProfessional TRIO control panel cccccceeccseee sees eese cece cess eeeeeceeeeaeeteeeseeenes 17 4 4 The High Performance Smart Lid HG 18 3 OPEO BEE 19 5 1 The TProfessional TRIO user mmtertace nnn 19 5 1 1 TProfessional TRIO menu buttons nenn nnnenennnnnn nenn ennnnen 19 5 1 2 TProfessional TRIO softkeys nenn nano nenn nnnnennnen nennen 19 5 1 3 Login MENU en een
43. ena Company 11 Service Should you have any problems with this unit please contact our service department or your local Biometra distributor Biometra GmbH Service Department Rudolf Wissell Stra e 14 16 D 37079 G ttingen Phone 49 0 5 51 50 68 6 10 or 12 Fax 49 0 5 51 50 686 11 e mail Service biometra com A If you would like to send the unit back to us please read the following return instructions in chapter 11 1 11 1 Instructions for return shipment In case of an instrument failure that cannot be fixed by the procedures described in section 8 please proceed as follows e Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 551 50881 10 12 Fax 49 551 50881 11 e mail service biometra com e Please contact our service department for providing a return authorization number RAN This number has to be applied clearly visible to the outer box Returns without the RAN will be not be accepted e mportant Carefully clean all parts of the instrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device e Please prepare written confirmation that the devi
44. ennnnnnnenennenenennen 24 70 System configuration 61 System mio 63 SYSTEM menu 21 System settings sse 59 Technical epechcatons 8 Temperature OopDmisation 32 Time and Dele EE 62 USB COPY DroOram 55 56 delete program 55 FUNCIONS asaisoieneneenereenernererrrrerrererrerenne 53 max program number of ODEN te le TEE 53 User account LIEBTE oue len EDIDI EDI RE QUE 24 deiere 25 71 72 User Account Deleie sisreroneroorie n nTen ES 60 Leer miertace 19 MY ALLAN NV EE 89 Instruction Manual TProfessional TRIO 12 2011
45. er by a serial cable mode full handshaking to a computer and start the hyperterminal Press OK to start the data transfer User BS 01 08 11 16 09 45 Create service into file For diagnosis by Biometra Service create service info file Connect cycler with USB to PC open Hyperterminal enter SINF ta create service infa file Email service infa file ta Biarnetra Overy leu Send the SINF Service Info File by email to the Biometra Service Department see chapter 6 5 6 5 How to contact Biometra This screen shows information how to contact Biometra Instruction Manual TProfessional TRIO 12 2011 69 Biometra An Analytik Jena Company ser TU 31 05 10 16 01 5 How to contact Biometra Biometra GmbH Rudaolf issell 5tr 30 37079 Goettingen Ger mary 49 07551 50686 0 49 0 551 50666 66 e mail infom biometa com www biametra cam D very j pu 6 6 Supervisor system configuration The supervisor has access to an own system menu with some specific functions 6 6 1 Change user PIN The supervisor can change the PIN for each user account Select the appropriate user directory from the list and press Select User User SUP 31 05 10 17 09 SUP Change user PIN select user Biometa Shared SIR Supervisor TU Testuser Enter a new PIN and repeat the PIN code Confirm with PIN OK TO Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company User SUP 31 05 10 17 10
46. ervisor Use the cursor keys to select a language and confirm with Select Language The Default Language is English User SUP 01 06 10 09 13 SUP Set boot language Set boot language Deutsch Language Language 6 6 5 Delete all user accounts The supervisor can delete all user accounts and including all programs In the next screen confirm with OK to delete all accounts 12 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company User SUP 01 06 10 09 16 System configuration Supervisor topic list 1 Change user PIN Delete all programs and user accounts from memory Warning restore memory isn t possible Note Deleted user accounts and programs can not be restored Instruction Manual TProfessional TRIO 12 2011 13 Biometra An Analytik Jena Company 7 Short manual Safety Warnings Do not open the instrument unless you are authorised to do so Check the label at the backside if the instrument has correct voltage configuration 110V 115 V or 230 V see chapter 3 4 The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the
47. g the boot process the TProfessional TRIO hardware is checked power on self test The results of the last log file are stored and can be displayed User TU 71 05 10 4 1 View logfile of poweron selftest Power on test OK Hardware Initialisation OK Controllertest OK Memory check OK Sensor calibration check OK 0 3 2 Messages NO Last power on 30 05 10 08 05 55 Last power down 30 05 10 18 09 09 Power on D very pu Messages 6 4 2 Execute block extended self test In addition to the regular power on self test a more comprehensive extended block self test can be triggered by the user In the extended block self test the unit can check the status and condition of different components and functions Therefore this test is an important tool and gives useful information to the customer whether the Thermocycler is still working within its specifications To initiate the extended block self test press Execute Selftest The test takes approximately 20 minutes During the test no programs can be run on the thermocycler 66 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company ser TU 31 05 10 47 Execute extended black selftest Check block performance with extended selftest Insert microplate or two rovs of tubes and start test with closed pressed heated lid Test will take about 20 minutes You can t run programs during executian At certain intervals the unit will auto
48. in case of blocked lid When the lid is in the very up or down position it may happen that the wheel is uncoupled In this situation the clutch mechanism is active in both directions clicking noise in either direction Instruction Manual TProfessional TRIO 12 2011 19 Biometra An Analytik Jena Company To unlock wheel press down metal pin with a ball pen and turn wheel carefully This pin overrides the automatic clutch mechanism Thus care must be taken not to apply excessive pressure Release lid in upper position 1 press pin 2 carefully turn wheel while holding the pin down CLOCKWISE until you feel normal resistance no more clicking noise clutch is released Release pin and turn lid down until the clutch mechanism is activated clicking noise optimum pressure applied Release lid in down position 1 press pin 2 carefully turn wheel while holding the pin down COUNTER CLOCKWISE until you feel normal resistance no more clicking noise clutch is released Release pin and turn wheel counter clockwise until pressure is completely released Open lid Important When the clutch mechanism is active optimum pressure is applied do not use pin to further increase lid pressure This would lead to damage of tubes and instrument 80 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 9 Maintenance and repair 9 1 Cleaning and Maintenance The TProfessi
49. in use The program will be started simultaneously on all blocks when the Start button on the control panel see also chapter 4 3 is pressed 5 4 Edit programs Programs can be edited in the programming spreadsheet or graphical mode To toggle between these two modes use the softkeys Edit Table or Edit Graph respectively The graphical mode is intended for easy modification of existing programs which can then be saved with a new program number and name To edit programs in the graphical mode see section 5 4 5 Note During operation of the Thermocycler the active program can be viewed but not modified If you want to change settings of the active program for further experiments you have to save a copy of this program For further information about copying programs see section 5 4 3 5 4 1 Insert program step Move cursor to the position where a new step should be inserted Edit program TU 01 test 25 07 11 Preheat Lid OM 99 c Block type combi 3548 S l 55 se 950 ml 5 0465 0100 30 720 jooj 160 jPase Save As To insert a program step press softkey Insert Delete Step The step at which a new step will be inserted appears highlighted 34 Instruction Manual TProfessional TRIO 12 2011 Press Insert Step Biometra An Analytik Jena Company User TIJ 26 07 Edit program TU 01 test 25 07 11 Preheat Lid OM 99 C Block type Combi 3548 REESEN so ea 55 04
50. mated End 13 23 Estimated End 13 23 wLid 99 0 cC Skip Step Skip Step Skip Step Black 1 3 To toggle between the modes use softkeys Show Time Show Graph and Show Table Lid 99 0 C Overview Block 1 3 CO very i phy Block 1 3 To access the block temperature overview screen press Overview Temperatur User TU 31 05 10 12 01 Overview Blockselection speed dial with no Block 1 Sri Block 3 active Pause free TU Ue pcr z step 1 93 0 95 0 C 3d Lid 99 0 C TU 03 rt pcr Step 1 20 0 SOUPE Jd Lid aa n c Overview Times The actual block and lid temperature and step are displayed 48 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 5 6 5 Pause Program during run To pause a program press the corresponding softkey Block 1 Block 2 or Block 3 User TU 71 05 10 Select block with cursor right left or softkeys Block 1 Block 2 active active TUI TU Ue pcr 2 03 rt pcr Testuser TU Testuser TU 1h4 m h 55m 13 27 12 35 Temperatur Press Pause Block Skip Step User TIJ 31 05 10 Frogram BIk 1 active niu Lid 99 0 C Pause B UL Overy jay Skip Step Block 1 3 Press Pause Block User Tu 31 05 10 Pragrarm BIk 1 active niu Pause Block Dr Skip Step Skip to step 2 Press Back to cancel Instruction Manual TProfessional TRIO 12 201 1 49 Biometra An Analytik Jena Compa
51. matically recommend to check the components of the unit i e to execute an extended block self test message 729 Information Execution of extended block test is recommended 729 MEI Press Quit to confirm that the recommendation has been noticed In case the unit is switched off but the extended block self test has not been initiated the message will be shown again after the unit is switched on It is recommended to execute the extended block self test regularly 6 4 3 View log files from extended self test The results of the extended self test log files are stored by the instrument To view a summary of the self test select the suitable log file from the menu using the cursor keys and press Select Instruction Manual TProfessional TRIO 12 201 1 67 Biometra An Analytik Jena Company User TU 71 05 10 4 3 Select logfile extended selftest Date Time Block type 12 09 05 09 09 Unknown Unknown Unknown Unknown Unknown Serialno 12345627 123450857 2597 00067 6006 20 3852485721 The next screen summarizes the results of the extended block self test at a glance and gives an overview for the actual status of all important system components User TU 31 05 10 15 40 4 3 View logfile of extended block selftest COver all performance block OK Serialnn 12345627 Blocktype Unknown Execution period 09 09 11 25 12 09 05 Check heating lid OK Check cooling element OE Check thermal tracking Ok
52. metra An Analytik Jena Company User TIJ 26 07 Edit program TU 01 test 26 07 11 Preheat Lid OM 99 C Block type Combi 3548 e ee 350 oo 55 a c5 ox 720 sol 150 Pawel Save As Alternatively move cursor to the desired temperature optimisation step and press Edit TempOpti ser TU 26 07 Edit program TU 01 test 25 07 11 Edit temp optimization in step 3 6 Temperature optimization only with all 3 blocks Annealingtemp amp 0 0 C Increment ik Save As In the temperature optimisation screen an annealing temperature and an increment can be set The annealing temperature defines the temperature at this step for block 2 whereas the increment defines the temperatures for block 1 and block 3 Confirm the settings with Enter After the settings are confirmed the temperatures for the three blocks at this step are displayed To save the program press Save Save as For easy recognition by the user all temperature optimisation protocols get the extension TOpt Instruction Manual TProfessional TRIO 12 2011 33 Biometra An Analytik Jena Company Select program name or enter program number Han lii rw m O2 pcr 2 25 07 11 Us rt pcr 25 07 11 O4 demo 25 07 11 05 free 06 free Edit Program Note To start programs containing a temperature optimisation step all three blocks are required Therefore please ensure that none of the blocks is currently
53. mperature optimisation step programs with extension TOS all three blocks are required Therefore please ensure that none of the blocks is currently in use see chapter 5 3 10 5 6 2 Quick start programs from the block menu The TProfessional TRIO provides the last 5 programs that haven been run for quick start Press the Block menu button above the display see chapter 5 1 2 The block selection screen opens User TU Select block with cursor right left or softkeys Block 1 Block2 Block 3 free free free Overview Temperatur To select a block press the corresponding softkey or use the cursor keys left right Then select one of the last five programs with the cursor keys ser TU Block 3 selected Block type Combi 3548 Latest programs edited run by TU 1 TU Iz pcr 2 2 TU 01 test 20 07 11 TOS Black 1 3 Selection Program Optionally programs can also be started on several blocks simultaneously To select more than one block press Multiblock Selection Instruction Manual TProfessional TRIO 12 2011 45 Biometra An Analytik Jena Company User TU Select multiple blacks enter no To start programm TU 02 pcr 2 on multiple blocks select further blocks with softkeys and press Confirm selection Block 1 Block2 Block 3 free selected selected Confirm selection Select deselect blocks with the corresponding sofkeys Confirm with Confirm selection Press the Start button on the left
54. n with OK or press Cancel to cancel the deletion User AA 19 12 11 Select program Li USB Drive Delete program L Aya_Olno name CANCEL Note Deleted programs cannot be restored 5 8 4 Copy program from USB drive To copy a program select a program file form the displayed list and press Copy Program see chapter 5 8 2 Select a storage place in your user directory and press Save Copy ser AA 13 12 11 Lopy program AWA 01 no name to Li C3 use Drive LC AWA Awa 16 Programs 13 30 stepsa 13 12 11 14 na name 14 12 11 15 tadjmtw 15 12 11 16 jmagj 18 12 11 TO Name The name of the program that is going to be copied can be changed prior storage Press Edit Name and in the next screen enter a new name for the program Confirm with OK or press Cancel to cancel the name change Instruction Manual TProfessional TRIO 12 201 1 55 Biometra An Analytik Jena Company ser Aa 19 12 11 Lopy program AWA 01 no name to Li USB Drive FC Enter program name Maximum 15 letters Save copy to USB Changes won t be saved CANCEL 5 8 5 Copy program to USB drive To copy a program to the USB drive select a user directory and press Copy Program ser TI 19 12 11 Select program name or enter program number Li I use Drive 01 14 10 11 U2 pcr 2 26 07 11 Us rt pcr 25 07 11 04 free 05 free 06 free Cony Delete Edit Program Program
55. nd press menu button Program above the display to enter the TProfessional TRIO program menu The directory Biometra Shared contains several pre installed protocols for different PCR applications ser TU 27 05 10 Select directory HEN zum nl 25 Biometra Shared I SUP Supervisor 7 TU Testuser Block l Directory Press Open Directory to open the directory Biometra Shared Choose a suitable program by navigating with the cursor keys or enter the corresponding program number Protocols may be started directly from the Biometra Shared directory or may be copied to the respective user directory and edited see chapter 5 4 3 Note Please always before starting a template protocol check the single steps and adapt details times temperatures loops temperature and time increments according to the required PCR conditions respectively Biometra will not be liable if the PCR does not work if necessary adjustments have not been programmed Instruction Manual TProfessional TRIO 12 201 1 25 Biometra An Analytik Jena Company Note The directory is protected by a PIN Code The default PIN for the directory Biometra Shared is 0000 5 3 2 Create program without template Press menu button Program above the display see chapter 5 1 to enter the TProfessional TRIO Program menu User TIJ 27 05 10 Select directory Hau ERN nl I BS Biometra Shared I SUP Supervisor air Testuser Program Directory Pres
56. ny The message Pause for the corresponding block is displayed User TU 71 05 10 Select block with cursor right left or softkeys Block 1 Sri Block 3 Pause active free TUI TU Ue pcr 2 03 rt pcr Testuser TU Testuser TU 1h4 m h 55m 13 52 13 00 5 6 6 Continue program To continue a program press the corresponding softkey Block 1 Block 2 or Block 3 User TUI 31 05 10 Program Pause Bik 1 mer D very pu Black 1 3 To continue the paused program press softkey Continue 5 6 7 Skip program step To skip a program step press the corresponding softkey Block 1 Block 2 or Block 3 50 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company User TU 31 05 10 Select block with cursor right left or softkeys Block 1 sitet era Block 3 active active free TUI TU Ue pcr 2 03 rt pcr Testuser TU Testuser TU 1h4 m h 55m 15 15 14 23 Temperatur Press Pause Block Skip Step User TIJ 31 05 10 Pragrarm Bik 1 active iiu Lid 99 0 C C very pu Black 1 3 Press Skip Step User TIJ 31 05 10 Pragrarm BIk 1 active iiu Pause Block Dr Skip Step Skip to step 2 Press Back to cancel The actually performed step will be skipped The program continues with the next step Instruction Manual TProfessional TRIO 12 2011 51 Biometra An Analytik Jena Company 5 7 Stop program To stop an active program press
57. o 160 Pause Note The new step has been deleted within an existing loop This loop now consists of only 3 steps 5 4 3 Copy program It is possible to copy programs from other user directories into the own user directory Press menu button Program above the display and select a user directory Press Open Directory ser TU 25 07 Select directory 7 Ee Biometra Shared I SUP Supervisor 7 TU Testuser Block P l Directory Select a program to copy with the cursor keys Instruction Manual TProfessional TRIO 12 2011 37 Biometra An Analytik Jena Company ser TIJ 6 07 Select program name or enter program number mo 03 LC BS Biometra Shared 3 Programs O1 test 26 07 11 TOS Ue rt per 25 07 11 SOs dema 04 free 05 free 06 free Program Press softkey Copy Program The cursor automatically jumps to the next available free program store in the directory of the currently logged in user ser TU 6 07 Copy program BS 03 demo to Testuser 3 Programs 01 test 6 07 11 TOS DE pcr 2 6 07 11 Us rt pcr 25 07 11 dera 05 free 06 free The copied program can be stored at any storage number To change storage number move the cursor to the wanted location or directly enter a number two digits atthe keypad If desired edit the program name Edit name Confirm to save the program with Save Copy 5 4 4 Delete program Select
58. o Biometra standard procedure Capacity increases to 35 x 0 5 ml tubes by use of small cap tubes 8 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 1 4 Legal Notes 1 4 4 PCR License Legal Disclaimer Purchase of a Biometra Thermocycler conveys a limited non transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of US Patents Nos 5 038 852 5 656 493 5 333 675 5 475 610 and 6 703 236 or corresponding claims in their non US counterparts owned by Applera Corporation No right is conveyed expressly by implication or by estoppel under any patent claim reagents kits or methods such as 5 nuclease methods or under any other apparatus or system claim including but not limited to US Patent No 6 814 934 and its non US counterparts which describe and claim thermal cyclers capable of real time detection Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Biometra biomedizinische Analytik GmbH Rudolf Wissell Str 30 PICIR 37079 Goettingen Germany icensed and authorized 1 4 2 Copyright All rights reserved It is not allowed to copy and publish the manual or parts of it in any form as copies micro film or other methods without a written authorisation from Bi
59. of electric shock Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains Connect the TProfessional TRIO Thermocycler to a grounded socket When only few samples are put in the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Appropriate safety regulations must be observed when working with infectious pathogenic or radioactive material Ask the responsible local safety inspector for details The TProfessional TRIO Thermocycler must not be used with explosive flammable or volatile liquids Do not place fingers between lid and housing when opening or closing the lid Before opening of the lid release lid pressure see section 4 4 It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used Ensure that both the rear and bottom ventilation slits not clogged by dust or other material Danger of overheating Let equilibrate the TProfessional TRIO Thermocycler to room temperature before starting operation There must be sufficient distance between the ventilation slots on the rear of the Thermocycler and a wall o
60. ometra Biometra is pointing out that applied company and brand names are usually protected trade marks 1 4 3 Liability Biometra is not liable for damages and injuries caused by use not considering these operating instructions in parts or completely 1 4 4 Meaning of the instructions Biometra recommends that you first read these instructions carefully This operation instruction is part of the product and should be kept over the full life time of the instrument It should also be forwarded to subsequent owners and users Make sure that additions and updates are inserted into the operation instructions Instruction Manual TProfessional TRIO 12 201 1 9 Biometra An Analytik Jena Company 2 Safety and Warning Notices 2 1 Definition of Symbols Symbol A A A A Definition Caution Refer to instruction manual Danger High voltage Fragile Danger Hot surface 2 2 General Safety Instructions Please read this manual carefully before starting operation of the TProfessional TRIO Thermocycler The TProfessional TRIO Thermocycler is intended for sample incubation at varying temperatures General safety precautions for laboratory work must be observed when working with the TProfessional TRIO Thermocycler The TProfessional TRIO does not produce a sound power level that could be hazardous for the user bbbBPE The thermoblock and the heated lid will reach high temperatures during operation Both thermo
61. on Manual TProfessional TRIO 12 201 1 57 Biometra An Analytik Jena Company The maximum number of program on the USB drive is limited to 48 If this limit is exceeded a message will be displayed 58 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 6 System settings To change global settings press the System menu button above the display see chapter 5 1 User TU 71 05 10 Topic overview select topic or enter topic no 1 User Configuration change language change PIN delete user System Configuration beep contrast set date time defragment memory screen saver System Info run logfile serial nurnber black info software versions Service selftest execute extended block selftest create Service Info File error log How to contact Biometra The system menu main screen opens Choose a single menu item using the cursor keys then press Select 6 1 User configuration These functions allow changes to individual user accounts User TU 31 05 10 14 08 1 System Configuration User select topic 11 Set language 12 Change PIN 13 Delete account All programs in user directory will be deleted Choose a single menu item using the cursor keys then press Select or press Overview to go back to the system main menu 6 1 1 Set language The language can be set and stored individually for each user account Available languages are English and German
62. onal TRIO was built to operate for a long time without the need for periodical maintenance Nevertheless occasionally cleaning of the air inlet may be necessary to maintain the efficiency of the Thermocycler Insufficient airflow may lead to reduced heating and cooling rates The inlet for the airflow is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner Additionally the Thermocycler housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning Never treat silver block with abrasive agents Note Appropriate safety regulations must be observed when working with infectious or pathogenic material 9 2 Servicing and repair The TProfessional TRIO Thermocycler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal The Service department offers Thermocycler maintenances and temperature verifications Biometra recommends an annual maintenance and a biannual temperature check for all Thermocyclers Please call the following phone number for detailed information 49 551 50881 10 12 9 3 Firmware update For in
63. r another instrument min 10 cm Danger of overheating This instrument is designed and certified to meet EN 61010 1 safety standards It should not be modified or altered in any way Alteration Instruction Manual TProfessional TRIO 12 201 1 11 Biometra An Analytik Jena Company of this instrument will void the warranty void the EN61010 1 certification and create a potential safety hazard Place the TProfessional TRIO Thermocycler on a stable non flammable surface in a dry safe environment For details see working conditions in table Technical specifications see chapter 1 3 Do not use alcohol e g methanol ethanol organic solvents or abrasives to clean the instrument For transports always use the original Biometra box Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company 3 Installation 3 1 Content of delivery 1 Thermocycler 2 Mains connector 3 Manual 4 Short Manual Please keep the original transport box for return shipment in case of servicing The TProfessional TRIO shipping box provides a specially developed system for contact free transport of this electronic device 3 2 Unpack and check Unpack and carefully examine the instrument Report any damage to Biometra Do not attempt to operate this device if physical damage is present Please keep the original packing material for return shipment in case of service issues A I Attention Please fill out an
64. rview of the used program press View Program for a comprehensive of all messages for this run press View Messages User BS 29 07 11 12 05 3 2 Run logfile TW 01 Test 1 Log Time and Date 10 06 25 07 11 Start Endtime Date 08 07 10 05 25 07 11 User Wht Wilhelm Serial Wo 1254555 Company Biometa Cyclertype TPrafessional Trio Software Yersion 0350 2 00 2 00 1 20 Block 2 Block Serial No 34546789 Blocktype Combi 3548 Messages Auto restart at 09 30 25 07 11 In step 3 loop 63 Freeze Program started at 09 31 25 07 11 The messages are listed by date and time If a power failure takes longer than 30 minutes a pause step at 4 C will be started automatically as soon as the power is back Press View Freeze Prog to view the freeze step ser PJO 70 07 11 4 56 BIETEN oO OF 11 3 Messages Block 2 IW 01 Test 1 View program 01 freeze 03 07 04 Date Time Message PreheatLid OFF C Block type Combi 3548 23 07 11 10 05 300 1 23 07 11 08 30 706 1 3 141 63 Freeze Prog sell Instruction Manual TProfessional TRIO 12 2011 65 Biometra An Analytik Jena Company 6 4 Service This menu provides several service functions for the instrument ser FRA 12 01 08 4 Service select topic 41 Poweron selftest logfile 4 2 Execute extended block selftest 43 Extended block selftest logfiles 44 Error Logfiles 45 Make Service Info Package 6 4 1 View log files of Power on self test Durin
65. s softkey New Program The easy programming spreadsheet opens see chapter 5 3 3 5 3 3 Set lid pre heating mode Before the program starts the lid can be pre heated The pre heating ensures the formation of a homogeneous tempered air cushion between the samples and avoids evaporation during the initial heating phase Note During the pre heating phase of the lid the block is held constant at 25 C Note After reaching the set lid temperature there is a 40 second equilibration phase before the block starts Note The default setting which is recommended for most applications is pre heating ON User TU 26 07 11 Edit program TU 01 Preheat Lid EM C Block type Combi 3548 26 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company However if the program should already start while the lid is heating up set the pre heating mode OFF using softkey Preheat ON OFF Confirm the settings for the heated lid with Enter or step to the next field with the right cursor Key 5 3 4 Set lid temperature Note Thanks to the design of the High Performance Smart Lid lower lid temperatures can be used for thermocycling than in the past Thanks to the lower lid temperature a higher temperature uniformity between samples is be achieved User TI 20 07 11 12 07 Edit program TU 01 Preheat Lid ON Block type Combi 3548 The recommended lid temperature is 99 0 C Enter temperature and confirm with
66. space conomique europ en qui regroupe les Etats membres de l UE et la Norvege Islande et le Liechtenstein Instruction Manual TProfessional TRIO 12 201 1 87 Biometra An Analytik Jena Company 14 EU Konformit tserkl rung EU Declaration of Conformity EU Konformit tserkl rung EC Declaration of Conformity G ttingen August 2011 im Sinne der EG Richtlinie ber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 2006 95 EG following the EC directive about electrical equipment for use within certain limits of voltage 2006 95 EC und and im Sinne der EG Richtlinie fur die elektromagnetische Vertraglichkeit 2004 108 EG following the EC directive about the electromagnetic compatibility 204 108 EC Hiermit erklaren wir dass folgende Thermocycler Herewith we declare that the following Thermocyclers Typen types TProfessional TRIO 30 TProfessional TRIO 48 TProfessional TRIO combi Best Nr Order No 070 720 070 723 070 724 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 2006 95 EG und der EC low voltage directive 2006 95 EC and the EG Richtlinie ber die elektromagnetische Vertraglichkeit 2004 108 EG entsprechen EC directive about the electromagnetic compatibility 204 108 EC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN61326 EN61000 3 2 EN61000 3 3
67. struction for firmware upgrade please contact the Biometra Service Department or your local distributor sales representative 9 4 Replacement of spare parts Only original spare parts mentioned in these operating instructions are allowed Instruction Manual TProfessional TRIO 12 201 1 81 10 Accessories 10 1 Plasticware Biometra An Analytik Jena Company Biometra offers a broad range of plasticware for the use in PCR The following table provides an overview for the product portfolio 82 Cat no 050 310 050 320 050 254 050 255 050 225 050 232 050 213 050 253 050 231 050 240 050 237 050 256 050 257 050 236 050 194 050 694 050 251 050 252 Description Single tubes 0 2 ml tubes with caps 0 5 ml tubes with caps Tube strips Strips 8 tubes and flat caps Strips 8 tubes and domed caps 48 well plates 48 well microplate 96 well plates 96 well skirted 96 well non skirted low profile 96 well non skirted 384 well plates HSQ 384 well skirted 384 well microplate Miscellaneous Silcone mat Adhesive film Heat Sealing Film Heat Sealing Film Aluminium 96 well aluminium plate 384 well aluminium plate Nop mat 96 well Nop mat 384 well Quantity 1000 pcs 1000 pcs 125 pcs 125 pcs 50 pcs 25 pcs 25 pcs 25 pcs 50 pcs 50 pcs 50 pcs 100 pcs 100 pcs 10 pcs 1 pcs 1 pcs 20 pcs 20 pcs Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik J
68. struments life me 2 To reduce or increase the annealing temperature from step to step within a loop set a negative or positive temperature increment in the column AT C 2 To increase or reduce the incubation time from step to step in a loop set a positive or negative time increment in the column At s 2 he ramping rate heating and cooling is pre set according to the block type To adjust the ramping rate enter any value in steps of 0 1 C in the column A C s 2 To define protocols including a gradient like temperature optimisation step enter two temperatures for block 1 and block 3 separated by a minus in the column C e g 50 60 or press TempOpti This activates the screen for programming temperature optimisation steps User BS 24 07 11 Edit program BS 01 test 25 07 11 Edit temp optimization in step 3 6 Temperature optimization only with all 3 blacks Annealingtemp Increment 5 0 C 23 0 C 60 0 C 65 0 C gt Enter an annealing temperature and an increment The annealing temperature defines the temperature for block 2 The increment defines the temperatures for block 1 and block 3 The resulting temperatures for block 1 3 are shown below Note To start programs containing a temperature optimisation step all three blocks are required 76 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 2 Press Save Save as to save a program Insert Deiete Edit Edit Sa
69. th german law i e 871 StrlSchV 817 GefStoffV and S19 ChemG and to avoid exposure to hazardous materials during handling or repair please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS PHONE NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Method of cleaning decontamination The equipment has been cleaned and decontaminated NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER Instruction Manual TProfessional TRIO 12 201 1 85 Biometra An Analytik Jena Company General Information for Decontamination Please contact your responsible health amp safety officer for details Use of radioactive substances Please contact your responsible person for details Use of genetically change organism or parts of those Please contact your responsible person for details 86 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company 13 Note for the disposal of electric electronic waste Note for disposal of electric electronic waste Hinweis f r die Entsorgung von Elektroaltgeraten du traitement des
70. u button above the display Open a user directory Open Directory and select the program to start Press Choose Block User TU 02 08 11 Select program name or enter program number Bas 02 FC TU Testuser 3 Programs 01 test 26 07 11 TOS Us rt pcr 26 07 11 04 free 05 free 06 free Edit Program To choose a block press the corresponding softkey Instruction Manual TProfessional TRIO 12 201 1 43 Biometra An Analytik Jena Company User Tu 02 08 11 Select program enter no block Dan LL 02 FC TU Testuser 3 Programs 01 test 26 07 11 TOS Us rt pcr 26 07 11 04 free 05 free 06 free Edit Program 17 08 11 Block 1 selected Block type Combi 3548 Latest programs edited run id TU 1 TU 02 pcr 2 2 TU D1 test 26 07 11 TOS Black 1 3 Selection Program Optional To select several blocks use the function Multiblock Selection Confirm the selection with Confirm Selection ser TU 02 085 11 Select multiple blocks enter no To start programm TU 02 pcr 2 on multiple blocks select further blocks with softkeys and press Contirm selection Block 1 Block 2 Block 3 selected selected free Confirm selection To start the program on the selected blocks press the Start button on the control panel see chapter 4 3 44 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company Note To start programs containing a te
71. un by TU 20 07 11 Block 1 3 Selection Program Press the Start button on the left side of the control panel see also chapter 4 3 to start the program on the selected block Optionally programs can also be started on several blocks simultaneously To select more than one block press Multiblock Selection User TU Select multiple blocks enter no To start programm TU 01 test on multiple blocks select further blocks with softkeys and press Confirm selection Block 1 Block2 Block 3 selected selected free Confirm selection Select deselect blocks with the corresponding softkeys or use the cursor keys left right Confirm with Confirm selection Press the Start button on the left side of the control panel see also chapter 4 3 to start the program on the selected blocks 5 3 10 Set temperature optimisation step For the optimisation of new PCR protocols very often gradient enabled Thermocyclers are used The TProfessional TRIO offers no gradient function but by the temperature optimisation step TOS function pograms can be created making use of the three blocks and delivering different annealing temperatures at the defined step The easiest way to define a temperature optimisation step is to enter two temperatures separated by Confirm with Enter or move cursor to the next field The set temperatures become separated by a step like icon 32 Instruction Manual TProfessional TRIO 12 2011 Bio
72. ve Sten pe Graph Save As Subsequently the program storage screen opens 04 Tip Biometra Shared 3 Programs A folder with common protocols could be O1 test 26 07 11 TOS established From the common folder each user 02 rt pcr 26 07 11 may copy any program into the own account and d 03 demo 26 07 11 modify it easily na name 05 free 06 free 2 By default the program is stored at the first free storage place available To save a program at a specific storage place use cursor keys up and down or enter two digits 2 To edit the name of the program press Edit name In the next screen enter a name by using the keypad and confirm with OK Start pause and stop programs 2 The TProfessional TRIO Thermocycler for each user stores the last 5 programs that have been run or modified For quick start of one of these programs press the Block menu button In the next screen select one of the last five programs by using the cursor keys up and down Press the Start button on the left side of the keypad to start the program 2 To start a program from a user directory log in to your user account and press the menu button Program In the next screen select the appropriate user directory and program by navigating with the cursor keys Press the Start button on the left side of the control panel to start the selected program After starting a program the following screen is shown ser TU 01 06 10 Select blo
73. ware reacts slower to user inputs By defragmentation the information becomes sorted and is stored in order thus leading to decreased memory access times Subsequently the software reacts faster to user inputs 62 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company User TU 71 05 10 2 4 Defragment memory To defragment memory press OF This may take several minutes Overy jew Confirm your selection with OK and wait until the defragmentation process is finished 6 2 5 Screen saver Set the screen saver on or off and if necessary change the time after the screen saver is started Press Save to save the settings 31 05 10 Screen saver alb ww starts after 30 min 30 60 min Overy jew The setting for Default is screen saver on and starts after 30 min 6 3 System info This menu provides information on the instrument and on the log files of the last nine runs Instruction Manual TProfessional TRIO 12 2011 63 Biometra An Analytik Jena Company User TU 31 05 10 3 System Info select topic 31 System Info serial numbers software versions 32 Run Logfiles last 9 runs Choose a single menu item using the cursor keys then press Select or press Overview to go back to the system main menu 6 3 1 System info This function displays information for the instrument like the serial number block type and software version System Info Hardware Info
74. ware update sss 81 Heated lid sess 26 27 um ex ARON 79 Konformitatserkla rung 88 LANgUagerse een 59 72 Beie RET 66 67 69 Boe MI MORTE ET 22 Log in menu eese 20 Maintenance ccccceecceeeeeeteeeeeeseeeeees 81 Operation Voltage 14 meom T a ee 84 DIN Code onnnonnonnonnonnennennennnn 23 60 70 71 tel AUC RR 02 Program CONUNUG f o0 CODING Tale NR RR ET 41 CODY M 37 25 elilcm 38 delete eren 36 LE 34 graphical mode 40 heating rate eeeeeeeeeeeseeeeee 41 JE SCD Mr 34 ero o MEE 20 era U m ER 29 49 quick start 45 SISTERE TEE 29 eR 50 90 Biometra An Analytik Jena Company spread sheet mode 40 lp p 43 ze 52 temperature increment 42 time increment oonsnenerneererrrerrerrrern 42 Program men ernennen 21 Repair een ee ne 81 Safety precautions o nennonnenneeneneenrenen 10 SAE CAV eege 63 Self test ka e e WE 66 Power on self test 66 SON E 66 Service info file 1 69 STACI reset S 81 Short manual 74 vl een nee 43 SUpervisOr eeeeneensnennen
75. with Enter Enter the number of back loops in the column loops User TU 11 Edit program TU 01 test 25 07 11 Preheat lid OM 99 c Block type combi 3548 s sn 955 Sal S50 joao hg 720 010 MENO b Options 720 sol 160 Pause Ee 3ranh Gave As 28 Instruction Manual TProfessional TRIO 12 201 1 Biometra An Analytik Jena Company The loop is shown as a bracket at the left side of the spreadsheet Note Total cycler number number of back loops 1 e g enter 24 for a total cycle number of 25 5 3 7 Cool below ambient temperature After the program has been finished samples can be kept at below ambient temperature Program a pause to keep the temperature for an undefined time see chapter 5 3 8 2 07 11 Edit program TU 01 test 26 07 11 Preheat Lid OM 99 C Block type Combi 3548 k EEE 0 o sss foosof 72 0 oroo z s4 Options 78 sol BET Save As Note To save lifetime of Peltier elements a temperature of 16 C for the final step rather than 4 C is recommended 5 3 8 Program Pause To hold a temperature for an indefinite time enter U After confirming with Enter the word Pause is shown 5 3 9 Save program Press button Save Save as 11 Edit program TU 01 test 25 07 11 Preheat Lid OM 99 c Block type combi 3548 s sn 955 Sal 550 ol 720 euo 2
76. ycler features easy spread sheet programming This means that all program parameters are entered into a simple central spreadsheet without the need to toggle between different screens Four cursor keys provide easy navigation within the software The cursor keys LEFT and RIGHT have additional functions as described below The right cursor key moves the cursor to the next field lt gt This cursor can also be used to complete data entry By pressing the right SZ cursor key settings will be taken over and the cursor moves to the next field In the file directory this key moves to cursor forward to the next lower level The left cursor key moves the cursor back to the previous field p lt gt In most screens this cursor is equivalent to the Back softkey on the control NU panel see chapter 4 3 In the file directory this key moves the cursor back to the higher level 5 2 1 Login The TProfessional TRIO manages up to 30 individual users For each user a directory is created where programs are stored Programs from other directories i e users can be read and copied but not modified This allows to use all programs that are in the cycler memory but to write only in the own user directory Each user is identified by his user name max 15 digits and initials 2 3 letters Press menu button Log in out above the display A list of users is shown 27 05 10 To log in select user or enter first letter Barbara Micha
77. ycler to a grounded socket Prior to connecting the unit to the power source please ensure that the voltage selector at the back side of the instrument is set to the required voltage Danger of electric shock Unplug the power cable before you open the TProfessional TRIO Thermocycler e The display contrast can be adjusted to local light conditions 6 2 2 3 4 Operation Voltage Important Prior to connecting the TProfessional TRIO to the mains make sure that the setting of the Voltage selector is in accordance with your mains Voltage The TProfessional TRIO Thermocycler can operate at 100 115 or 230 Volt The operation Voltage is shown on the Voltage selector which is located at the instrument bottom To change operation Voltage of the TProfessional TRIO switch off the instrument and disconnect the mains plug Use a coin or another round shape item to turn the adjustment slot of the Voltage selector to the new Voltage Instrument bottom Instrument backside Voltage indicator BN Mains plug Fuses 7 Power switch 2 Adjustment slot 3 5 Initial self test After switching on the TProfessional TRIO the serial number of the instrument and the software version are displayed 14 Instruction Manual TProfessional TRIO 12 2011 Biometra An Analytik Jena Company Professional TRIO T Professional TRIO Thermocycler Thermocycler Serial Mo ExpWers Cyclertype TProfessional Trio

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