User Manual
Contents
1. DiluCell User Manual Thank you very much for your decision to use DiluCell in your laboratory DiluCell is especially designed for the usage with the OD6OO DiluPhotometer for analysis of samples at a wavelength range of 340 nm to 950 nm Due to the reduced pathlength DiluCell provides a virtual dilution without the need of a physical dilution of higher concentrated samples Dilution is mainly necessary to remain within the dynamic range of the ODGOO DiluPhotometer which should be 0 1 Abs to 1 0 Abs The two different available versions DiluCell 10 and DiluCell 20 allow an automatic 1 10 and 1 20 dilution of the sample The required sample volume is 200 ul or 100 ul respectively The following scheme explains the functionality of the DiluCell technology Lambert Beer Law I Abs log excxd Abs measured absorbance value on your instrument screen lo incoming light outgoing light extinction coefficient c concentration d pathlength 1 cm Virtual Dilution Factors of the DiluCell IMPLEN DiluCell DiluCell 10 gt d 1mm DiluCell 20 gt d 0 5 mm DiluCell Users Manual Ver 1 4 06 2009 Implen GmbH Munich Germany Page 1 DiluCell User Manual cont Operation Instructions Ensure the correct positioning of the DiluCell in your OD600 DiluPhotometer Make sure that the wide window faces the lightbeam Important Notes DiluCell will not work2. in any other orientation do not mix other cuvette types within a DiluCell measurement series for blank etc DiluCell is very easy to fill the geometrical setup Supports air bubble free sample application It is recommended however that you check for air bubbles before the measurement In case of an air bubble in the cell please remove it by gently tapping the cell Volume requirements are 200 ul for DiluCell 10 100 ul for DiluCell 20 Remember to multiply the reading from your sample after the measurement with the proper virtual dilution factor Virtual dilution factor are 10 for DiluCell 10 20 for DiluCell 20 Itis not recommended to reuse DiluCell due to high probability of cross contamination high probability of air bubbles when refilling the cell decontamination not easily possible for cell culture measurements e g Specifications Material PMMA Wavelength Range 340 nm 950 nm Volume Requirement 200 ul or 100 ul DC 10 or DC 20 Pathlength 1mm and 0 5 mm DC 10 and DC 20 at 15 mm center height Tolerance 3 DC 10 10 DC 20 Ordering Information DC 10 DiluCell 10 pack with 96 cells DC 20 DiluCell 20 pack with 96 cells Limitations Do not autoclave DiluCell The DiluCell is approved only for the usage with the OD6OO DiluPhotometer For solvent compatibility please contact your local Implen partner or the Implen Team at www implen de Featur
3. es and specifications are subject to change without notice DiluCell Users Manual Ver 1 4 06 2009 Implen GmbH Munich Germany Page 2
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