Moxi Z User Manual OS4
Contents
1. 20 MOXI ADDS sense NE en tous nt en nt AEE 21 ROW DIGSMOOUNG PP tones ered a sn tveuaal pha intone fasten E S 22 Maint nanee andSlOrAdR 2 sion sc scn nu E dates 24 Specifications for the Moxi Z Automated Cell Counter ccccccccccecceeeeeeeeeeeeeeeneeeneeeeeeeteeeneees 26 OPCS NO MIOMMANO Mics cascavecne OR RE A 28 TEGCHIMIGAlS CHV ICS an GR Sn A a a A 29 Waran ake ccc erences ne nee de banal ena ei dm eine cesarean 29 Introduction The ORFLO Moxi Z Mini Automated Cell Counter performs cell count and size measurements for particle sizes of 4 25 microns Type M cassette and 3 20 microns Type S cassette It also provides an assessment of mammalian culture viability using a proprietary software algorithm to report the standardized Moxi Population Index MPI It combines the gold standard Coulter Principle with a patented thin film sensor technology to deliver highly accurate and repeatable results in just 8 seconds Type M cassette or 15 seconds Type S cassette The instrument is ultra small and runs on a rechargeable battery making it ideal for use in a hood Cell concentration average cell volume average cell diameter and the Moxi Population Index MPI are displayed for each sample Test results are also displayed in the form of a histogram The Moxi Z unit can store approximately 500 histograms and if desired the data may be downloaded to a PC or Mac via a Bluetooth wireless connection or via USB2. Moxi The Mini Automated Cell Counter User Manual OS40 s lt The information in this document is subject to change without notice and should not be construed as a commitment by ORFLO Technologies a DBA of E I Spectra LLC Neither Orflo Technologies nor any of its affiliated corporations assumes responsibility for any errors that may appear in this document This manual is believed to be complete and accurate at the time of publication In no event shall Orflo Technologies or any of its affiliated corporations be liable for incidental or consequential damages in connection with or arising from the use of this manual CE 2012 ORFLO Technologies a DBA of E I Spectra LLC All rights reserved Rev 5 0 1 03 2012 The ORFLO symbol and Moxi are trademarks of E I Spectra LLC registered in the U S and other countries Windows Windows XP Windows Vista and Windows 7 are trademarks of Microsoft Corporation registered in the U S and other countries Mac and Mac OS are trademarks of Apple Corporation registered in the U S and other countries Bluetooth and the Bluetooth logos are registered trademarks of Bluetooth SIG Inc registered in the U S and other countries Accutase is a registered trademark of Innovative Cell Technologies T V SUD and the T V SUD symbol are registered trademarks of T V SUD Aktiengesellschaft Contents IMFO GUC HOME SR An en ee ii nat nn Ni eu 1 Symbols Used in This User Guide 1
3. 74 To perform a count 1 Insert cassette into MoxiZ 2 Pipette 7S5uL of sample into the cassette 3 Touch screen anywhere to start O ORFLO il IRAN LP Dec 16 14 03 00 Z Select a Histogram Dec 16 14 03 00 TEST 01 Curve Fit Count Average Size 9 218 um dia 1 55 pL vol i Ba Gating Delete 1 28e 05 Cells ml Dec 16 14 03 00 TEST 01 Gating Delete Dec 16 14 03 00 Select a ee SIT Close PageUp PageDown Delete ALL Dec 16 14 03 00 TEST 01 ao Arts ete Gating Dec 16 14 03 00 TEST 01 1 28e 05 mL 10 14 18 22 26 Diameter um _ Average Size _ 9 218 um dia 1 55 pL vol ORAN R Curve Fit Delete Gated Count 1 28e 05 Cells ml Jec 16 14 03 00 Gated Count Average Size 9 218 um dia 1 55 pL vol Gating Delete 1 28e 05 Cells ml Page 11 Dec 16 14 03 00 Dec 16 14 03 00 TEST 01 TEST 01 5 Tap the Average Size black box to toggle between diameter um and volume fL scales 2311 4608 6906 9203 Volume fL _Curve Fit Count __ Average Size _Curve Fit Count __ Average Size 3 9 2 im dia 9 25 im dia 1 28e 05 Cells ml c PACS 1 28e 05 Cells ml i Pa 25 pL vol 55 L vol OQA On a Back Gating Delete Back Gating Delete Dec 16 14 03 00 TEST 01 6 Press the Delete icon to permanently delete the results of the test Back Gating Dec 16 14 03 00 7 Press the Back icon to close the test results a a
4. Cassettes Refer to the Specifications section for information on the operating ranges for the system The sample volume for a test should be 75 uL If necessary prepare dilutions of the sample using ORFLO Diluent Cat No MXA006 or a diluent compatible with the characteristics of the cells The diluent used should not cause changes in the cell size and should have sufficient conductivity to enable operation of the instrument Water hypotonic or hypertonic solutions are not acceptable diluents Moxi Z Mini Automated Cell Counter User Manual Page 6 Notes Detergents may interfere with cell counting Additives such as DMSO or serum will alter solution conductivity and will result in corresponding changes of measured particle size Unfiltered media can contribute real particle counts that depending on size can overlap and alter cell counts Materials Required Cell sample diluted and dissociated if necessary 75 uL minimum Pipette and appropriately sized pipette tips Using the Moxi Z Automated Cell Counter Settings Set the date and time by pressing the Settings icon on the main menu of the Moxi Z Then use the arrow keys and follow the instructions displayed on the screen Cell Counting 1 If necessary dilute a cell suspension with a ORFLO Diluent or an appropriate diluent so that system oe the cell concentration is within the operating To perform a count range of the cassette being used Type M 2 Pipe
5. USB Cable Connects instrument to PC Mac or power adapter Power Adapter Connects USB cable to an AC outlet US and EU models only USB Flash Drive Stores Moxi Z software and user manual Calibration Check Beads Polystyrene beads for confirming proper system operation and calibration Electronic Calibration Cassette Electronic cassette for verifying proper system sold separately operation and calibration Installation The Moxi Z is shipped in a condition ready for initial use with the battery partially charged If necessary the battery can be charged by inserting the USB cable into the USB Cable Port and connecting the cable to a computer or power adapter If using a power adapter insert the plug into an AC outlet 110 220 V The battery will be fully charged in approximately four hours Note For optimal cell counting results perform cell counts with the USB cable disconnected Although the unit may be operated using AC power this mode may cause undesirable electrical noise which could result in less accurate small particle counting results and may affect the MPI When not in use the Moxi Z should be connected to a charging source so that the battery will maintain a continuous charge and be ready for immediate use If a low battery warning appears on the display stop using the instrument and charge the battery or operate the Moxi Z using an AC power source General Guidelines The Moxi Z Mini Automated Cell Counter is used with Moxi Z
6. full curve fitting technique it does not achieve quite the same level of accuracy As a result Orflo recommends the use of the curve fitting count mode over gating mode whenever possible in order to generate the true cell count 1 Except when running in Small Particle Mode the results of a test are initially displayed on a diameter scale of 4 34 um Type M cassette or 3 26 um Type S cassette To Change the x axis resolution touch the scaling button red arrow below The text below the button indicates the next scale range Successively pressing the scaling icon will cycle the diameter scale through the following ranges a 4 34 4 26 4 20 and 4 10 um for Type M cassettes b 3 26 3 18 and 3 10 for Type S o E 7 mPi 50 cassettes Note This feature is only available immediately following a cell count Touching the Done icon will save the current histogram with the current DE scale settings se eee aaa 1 28e 05 Cells ml s BL ait 1 28e 05 Cells ml um pLiv i 2 The histogram can also be gated manually by touching the Gating icon Gating markers can then be positioned as desired by touching and dragging each blue gating marker independently or by tapping the blue gating markers and using the arrows to adjust the gating ranges Switching between left and right gates can be accomplished by touching the middle black box with text Right or left gate location between the gating arrows Only the cells between t
7. instrument in an area with a high humidity level such as a greenhouse or an incubator Refer to Operating Environmental Conditions in Specifications section Do not touch the USB cable or USB charging adapter with wet hands e To avoid a potential shock hazard choose the correct plug configuration and make sure that the USB cable and USB charging adapter are plugged securely into a properly grounded AC power outlet Make sure that the connection between the USB cable and the instrument is secure e Always ensure that the power supply input voltage matches the voltage available in your location e Do not use with flammable or explosive liquids Do not immerse instrument body in liquid or allow liquid to enter any part of the instrument CAUTION e Do not expose instrument to vibrations Vibrations may cause instrument malfunction or damage e Do not autoclave or expose to high temperature e Use only authorized accessories universal power adapters USB cable e If the instrument is dropped and broken disconnect the USB cable and contact Orflo Technologies Do not attempt to disassemble the instrument Moxi Z Mini Automated Cell Counter User Manual Page 2 Consignes de s curit Veuillez lire et vous assurer de comprendre les consignes de s curit ci dessous avant d utiliser le compteur Moxi Z Cell A AVERTISSEMENTS e Afin d viter tout danger de choc lectrique ne pas installer l instrument dans un endroit
8. properly Do not remove cassette from Cell Counter before Lost sensor detect inserted into cell completion of counting cycle Ensure cassette is counter properly inserted Make sure concentration of cell sample is within Concentration of cell recommended guidelines Refer to General Warning High Concentration sample is too high Guidelines section Mise en garde concentration La concentration de S assurer que la concentration de l chantillon de lev e l chantillon de cellules cellules se situe dans la fourchette recommand e Se est trop lev e reporter la section Recommandations g n rales Start open Stop open Issue detected upon Reinsert sensor If problem persists return Start stop short test cassette insertion instrument for service Electrode short Previously used sensor Used cassette detected Do not reuse sensors Battery needs to be Recharge instrument for a minimum of 4 hours or use Low battery recharged instrument with AC power Instrument has exceeded maximum Disk full storage capacity of Delete histograms or download to computer 500 histograms After histogram is l recorded and saved Delete files Disk now full there is no more space on the disk drive Internal firmware issue Clear error by turning instrument off and then on due to instrument again Remove cell counter from sources of external Unhandled exception malfunction or high level interference If problem persists return instru
9. Page 19 Updating Moxi Z Firmware via USB requires Moxi Z OS v4 0 or greater The Moxi Z firmware can be updated through a USB connection For this method of update the user must connect the Moxi Z to the computer s USB port via the provided cable Note The cable is part of the AC power adaptor Unplug the power adapter to expose the USB connector for the computer Next the user needs to put the Moxi Z into a firmware update mode This can be done in either of the following two ways With the unit powered off quickly press the power reset button four times When successful the white Moxi Z M xi screen will display with text under the Moxi Z logo that The Mini Cell Counter indicates the system is in firmware update mode If unsuccessful turn the power off and retry ar 18 20 50 26 Mar 22 11 30 41 OR 0 ey os Select a Moxi App Alternatively in v4 0 or greater the firmware update ds eee mode can be entered from the Applications screen 2 Pipette Tad of sample into the cassette From the home screen touch the Apps icon and then touch the Loader icon If successful a white System is loading screen and the system is ready for updating ih leer 3 12 Moxi OS Loader V1 3 entered Hold the power button down for 5 seconds to power off System Is Loading From here open MoxiChart and select Update Firmware from the Tools menu Follow the instructions in the installation wizard to complete the firmware upda
10. Safety PreGautlonS EP RE 2 Consignes GE SC CUMMLC ssh rosiers tant A a an deneo detente sens anne tendu 3 Moxi Z Mini Automated Cell Counter Kit sisi 4 Moxi Z Mini Automated Cell Counter Accessories 5 HSAN enni R E E A E E EE eat 6 General SUITelINSS se eE A EE aes 6 Materials REGUE O eroien r EE de en Re T Using the Moxi Z Automated Cell Counter iii 7 SENO SR RU ladles alist th atten aachit abate dane etat sheen nada te ata bia ctheat ee tnciins 7 CellC OUMUNG 222s sata RSR a becs nude nae nea 7 Managing UNG DAS RS SR RE E Re te cine 8 Remevng and Deleting Dala ss nn Nr ed t eee 11 Moxi Z Performance Test Using Calibration Check Beads nnnannnnnnnnnnnnnnnnnsnnnnsnrsnrnnnrnnn gt 13 Moxi Z Performance Test Using Electronic Calibration Cassette cc ccccccceceeeeeeeeeeeneees 14 MOFA Old en aah N a easaeteieaeeeeh 14 Installing MoxiChart Software on a PC iii 15 Connecting to Moxi Z via Bluetooth with a PC 15 Connecting to Moxi Z via USB requires Moxi Z OS v4 0 or greater ccecceecceeeeeeeaeeenes 17 Installing MoxiChart Software on a Mac 18 Connecting to Moxi Z via Bluetooth with a Mac 18 Instrument Firmware and Software Upgrades ccccccecccseccseeceeeeeeeeeeeseeeeeeeeeeeeeseeseeeseees 18 Updating Moxi Z Firmware via Bluetooth MOXiChalt cccccceccceecceeeeeeece esse esse esse eeseeeeeeees 19 Updating Moxi Z Firmware via USB requires Moxi Z OS v4 0 or greater
11. cassette The Curve Fitting and MPI calculations begin automatically and require only a few additional seconds The results will then automatically be displayed on the screen To make Gating the default acquisition mode press the Curve Fit Count button to toggle into Gating mode pr TO mid v PINE D Managing the Data Background We have found based on a considerable amount of particle counting research that using a curve fitting approach to determine cell counts provides better count results than does a simple gating method Using a simple gating mode will often result in counts that deviate from true counts particularly at higher concentrations This is due to the occurrence of coincidence events or the simultaneous arrival of two or more particles cells in the sensing chamber A coincidence event despite the presence of multiple particles cells is electrically registered as a single spike or count event Moxi Z has a proprietary software algorithm based on curve fitting that adjusts for these coincidence events to determine the true count in the sample Data has shown that the Moxi Z coincidence correction algorithm generates Moxi Z Mini Automated Cell Counter User Manual Page 8 counts that closely correlate to that of the Coulter Z2 which has been widely regarded as the gold standard in counting technologies Moxi Z gating mode does implement elements of the Moxi Z coincidence correction algorithm but without the
12. connectivity v 4 0 The system is intended for research use only and has been tested with cell types that are representative of those commonly used The Moxi Z Mini Automated Cell Counting system is designed and manufactured by Orflo Technologies a DBA of E I Spectra LLC 130 Airport Circle Hailey ID 83333 www orflo com Symbols Used in This User Guide The following symbols are used throughout this user guide and or on product labels The user is responsible for operating the product in accordance with the indicated requirements Symbol Symbole Definition Definition Warning alerts you to actions that may cause personal injury or pose a physical threat La mise en garde vous alerte des actions qui risquent de causer des blessures corporelles ou de constituer une menace physique Do not discard with common solid waste at end of life Segregate with other waste electrical and electronic equipment WEEE and send to an appropriate facility for recycling Affixed in accordance with European Council Directives 2004 108 EC C electromagnetic compatibility and 2006 95 EC safety requirements Moxi Z Mini Automated Cell Counter User Manual Page 1 Safety tested and certified by TUV SUD Product Service Division Safety Precautions Please review and understand the safety instructions below before operating the Moxi Z Cell Counter A WARNINGS e To avoid danger of electric shock do not install the
13. ells are in a single cell suspension Break Cell clumping clumps by pipetting up and down with a standard pipette ORFLO recommends Accutase or equivalent Try running the cell sample at a higher concentration Cell concentration too low Refer to Managing the Data section for instructions on Peak of interest indistinct adjusting the Y axis Internal firmware issue due to instrument Reset instrument by pressing and holding the power Instrument stops responding malfunction or high level button for at least 5 seconds If problem persists return instrument for service of external interference i Battery is faulty or has Return instrument for battery replacement Battery will not fully charge surpassed its service life y rep Adjust pipette to ensure sufficient volume for the test Insufficient fluid lt 75 uL For improperly calibrated pipettes this might require a setting gt 75 uL Cells too larae Make certain that the cell type is within the specified pieomine ee J size range of the cassettes being used Highly strongly aggregated cells clogging filter and blocking flow Try breaking apart cells using pipette trituration and or protease treatment Moxi Z Mini Automated Cell Counter User Manual Page 22 Error Warning Messages Corrective Action Messages d erreur de Mesure corrective mise en garde Sample volume too Lost start small or air bubble in Make sure sample volume is 75 uL test cassette Sensor not
14. he markers are counted Auto Gating to a particular peak can be accomplished by toughing the display in the proximity of the desired histogram peak Touching the CurveFit icon will return the display to curve fit mode Dec 16 14 03 00 3 00 TEST 01 TEST 01 i era um vo Dor Gating 2 18ur De olete Moxi Z Mini Automated Cell Counter User Manual Page 9 Dec 16 14 03 00 3 Use the arrows to the right of the histogram to Me change the vertical scale of the histogram The vertical scale can also be changed by vertically swiping a finger on the display up to increase or down to decrease Curve Fit Count Average Size Gated Count Average Size 9 218 um dia aan 3 gt 9 218 um dia 1 28e 05 Cells ml 1 55 pL vol 1 28e 05 Cells ml 1 55 pL vol one N 2 amp Delete ne N amp 18um Delete Dec 16 14 03 00 Dec 16 14 03 00 4 Tap the Average Size black box to toggle nr SS eston between diameter um and volume fL scales 0 14 2311 4608 6906 9203 Volume fL _Curve Fit Count _ ms Curve Fit Count _ __ Average Size _ 9 2 1m dia 9 2 1m dia 1 28e 05 Cells ml 1 55 pL vol 1 28e 05 Cells mi 55 pL vol vier ae PAU Done Gating 2 18um Delete Done Gating 2 18um Delete 5 Press the Delete icon at any time to permanently delete the results of the test 6 Press the Done icon to save the results and return to the Home screen Note Ifthe total particle count of the test exceeds De CT the c
15. hoose the device that as corresponds to the Moxi Z Select a device to add to this computer Windows will continue to look for new devices and display them here Brother MFC 8480DN Desktop computer n 001ba94ccc0b Bluetooth 4 Web Services Ef Desktop computer zin BlueMod P2x G2 80 E3 Desktop computer Bluetooth Bluetooth a me Other Desktop computer sm Other Bluetooth Other What if Windows doesn t find my device 4 Ifa Moxi Z unit is not detected above screen is not displayed your Bluetooth radio most likely requires Bluetooth device pairing Windows and on Windows XP systems a Bluetooth serial port connection to be established As this process is specific to each Bluetooth manufacturer please follow their instructions to set this connection up Once established MoxiChart will automatically connect to that device on clicking the Bluetooth icon no device selection required 5 Select a folder to upload data files to Moxi Z Mini Automated Cell Counter User Manual Page 16 Connecting to Moxi Z via USB requires Moxi Z OS v4 0 or greater 1 Unplug the USB cable from the Moxi Z power adaptor 2 Plug the wide end of the cable into your computer s USB port 3 Plug the small end of the cable into the back of the Moxi Z Mar 22 11 53 16 4 Make sure the Moxi Z is powered on and wait for the Moxi Z to VS appear as a disk or flash drive in your computer s file system Wind
16. in order to receive notification about relevant firmware and software upgrades Moxi Z Mini Automated Cell Counter User Manual Page 18 Updating Moxi Z Firmware via Bluetooth Moxichart The Moxi Z firmware can be updated through a Bluetooth connection The user first needs to put the Moxi Z into a firmware update mode This can be done in either of the following two ways With the unit powered off quickly press the power reset button four times When successful the white Moxi Z screen will display with text under the Moxi Z logo that indicates the system is in firmware update mode If unsuccessful turn the power off and retry OR Alternatively in v4 0 or greater the firmware update mode can be entered from the Applications screen From the home screen touch the Apps icon and then touch the Loader icon If successful a white System is loading screen and the system is ready for updating Tz Moxi The Mini Cell Counter Moxi OS Loader V1 3 entered Hold the power button down powe To perform a count 1 Insert cassette into Moxiz 2 Pipette 7S5uL of sample into the cassette oxi OS Loader V1 3 entered M Hold the power button down for 5 seconds to power off System Is Loading From here open MoxiChart and select Update Firmware from the Tools menu Follow the instructions in the installation wizard to complete the firmware update Moxi Z Mini Automated Cell Counter User Manual
17. inated Use of the Electronic Calibration Cassette is described in the next section see page 14 It is recommended that calibration tests are run at 25 C For long term storage the Calibration Check Beads should be stored at 2 8 C Avoid freezing Moxi Z Mini Automated Cell Counter User Manual Page 13 Moxi Z Performance Test Using Electronic Calibration Cassette The Electronic Calibration Cassette Cat No MXA004 is an optional accessory that can be used to verify proper operation of the MOXI Z Simply insert the Electronic Calibration Cassette ECC into the MOXI Z and the test will begin automatically The ECC will generate data that simulates various cell sizes and concentrations Upon completion of the test a pass or fail result will be displayed as shown If repeated tests continue to generate a failed result contact your distributor or ORFLO Technologies for assistance 7 22 111 u 5 6 i 2 26um Ave Size m dia 60 pL vol Delete le Done Gating Moxi Z Help 1 For help with operation of the Moxi Z press the Help icon on the Home screen Visual instructions for inserting a cassette will be presented 2 Press the Next icon to view the next visual instruction that demonstrates how to pipette a sample into the cassette 3 Press the Next icon to proceed to the next instruction screen or press the Previous icon to return to the previous screen 4 Press the Done icon to exit the help screens and return t
18. iquide ne p n tre dans les cavit s internes de l instrument Maintenance There is no routine maintenance required for the Moxi Z Automated Cell Counter In addition there are no user serviceable parts Instrument repairs must be carried out by authorized personnel only Moxi Z Mini Automated Cell Counter User Manual Page 25 Specifications for the Moxi Z Automated Cell Counter Performance Sample Volume Required Dynamic Range Average Cell Size Average Cell Volume Cell Concentration Test Time Health assessment for mammalian cultures Software Moxi Z Firmware OS Dimensions Length Width Height Weight Electrical Specifications Internal Battery AC Power Adapters US and EU types 75 uL 2 34 um 4 25 um diameter Type M cassette 3 20 um diameter Type S cassette 34 8 180 fL Type M cassette 14 4 200 fL Type S cassette 3 000 500 000 cells mL Type M cassette 3 000 2 500 000 cells ml Type S cassette 8 seconds Type M cassette 15 seconds Type S cassette Moxi Population Index MPI PC or Mac compatible requires Windows XP Windows Vista Windows 7 or Mac OS X operating system v 10 6 or greater Version 4 0 as of 3 15 2012 Version 3 6 or greater required for Type S cassettes 7 6 in 19 3 cm 4 3 in 13 5 cm 2 8 in 7 1 cm 1 5 Ibs 680 g Rechargeable 3 7 V 4400 mAh lithium ion battery Input 100 240 VAC 50 60 Hz 0 2A Output 5V 1A CE certified Ope
19. literature may not be altered except by expressed written agreement signed by an officer of Orflo Representations oral or written which are inconsistent with this warranty or such publications are not authorized and if given should not be relied upon In the event of a breach of the foregoing warranty Orflo s sole obligation shall be to repair or replace at its option the applicable product or part thereof provided the customer notifies Orflo promptly of any such breach If after exercising reasonable efforts Orflo is unable to repair or replace the product or part then Orflo shall refund to the customer all monies paid for such applicable product or part ORFLO SHALL NOT BE LIABLE FOR CONSEQUENTIAL INCIDENTAL SPECIAL OR ANY OTHER DAMAGES RESULTING FROM ECONOMIC LOSS OR PROPERTY DAMAGE SUSTAINED BY ANY CUSTOMER FROM THE USE OF ITS PRODUCTS Moxi Z Mini Automated Cell Counter User Manual Page 29 Moxi Z Mini Automated Cell Counter User Manual Page 30
20. mated Cell Counter kit includes the Moxi Z instrument USB Cable Power Adapter US and EU versions only Cassette Dispenser Calibration Check Beads USB Flash Drive with software user guide and 25 Cassettes 2 tests per cassette Touch Screen Display Cassette Tray Power Reset Button USB Cable Port Cassette Test 2 Fill Port Test 1 Fill Port Cassette Moxi Z Mini Automated Cell Counter User Manual Page 4 Part Touch Screen Display Power Reset Button Cassette Tray USB Cable Port Cassette Function Allows user to interface with instrument Displays all information needed for operation Displays test results and histograms with curve fitting gating cell volume concentration diameter and Moxi Population Index MPI Turns Moxi Z on and off Resets the unit when pressed and held for gt 5 seconds Tray that needs to be pressed down for inserting cassette Connects instrument to USB cable Disposable used for loading samples Each cassette contains two ports thereby allowing for two samples to be run per cassette Moxi Z Mini Automated Cell Counter Accessories m Cassette Dispenser USB Cable amp Power Adapter USB Flash Drive with software Calibration Check Beads Electronic Calibration Cassette sold separately Moxi Z Mini Automated Cell Counter User Manual Page 5 Part Function Cassette Dispenser Stores up to 25 cassettes for convenient dispensing
21. ment for of external interference service SPI timeout Instrument malfunction If problem persists return instrument for service Corrupt filesys Instrument malfunction If problem persists return instrument for service Instrument has detected corruption or new Defaults loaded version of firmware and If problem persists return instrument for service reset all settings to factory defaults False start Sensor malfunction Use new sensor False stop Moxi Z Mini Automated Cell Counter User Manual Page 23 Error Warning Messages Corrective Action Messages d erreur de Mesure corrective mise en garde User touched the black Small Particle Mode lt 8 um box to start the test Re run test touching the main part of the start screen Small Particle Mode selected Tests can only be rescaled immediately after the run Userrescaedicsi If it is immediately after a run the user can cycle Incorrect x axis scale range through the scale ranges by pressing the scale range icon see Managing your data section Type S cassette run on Moxi Z Firmware 3 5 or Upgrade Moxi Z system firmware to v3 6 or later earlier Maintenance and Storage Storage Store the Moxi Z Cell Counter and Moxi Z Test Cassettes at room temperature in a dry environment Avoid exposure to ultraviolet light as it may discolor and or damage the instrument Charging the Battery The Moxi Z Automated Cell Counter contain
22. nd return to the Histogram viewer screen aa Press the Close icon to return to the Home screen note changes to histogram view will not be saved i i Test 4 Test3 iT I i t1 Tes Magl23 18 03 i m WS Delete Close PageUp PageDown Delete ALL Moxi Z Mini Automated Cell Counter User Manual Page 12 Moxi Z Performance Test Using Calibration Check Beads A 5 mL sample of Calibration Check Beads Cat No MXA005 is included with each MOXI Z Automated Cell Counter The calibration check beads consist of polymer microspheres of a known size in solution at a known concentration The beads can be used to test the system as well as for practice and troubleshooting For best results invert the bottle containing the calibration check beads several times immediately before use Load a 75 uL sample using the standard procedure described in the section entitled Cell Counting page 7 The particle diameter and concentration results recorded by the Moxi Z should be approximately equivalent to that specified on the label of the calibration check bead bottle If the results differ significantly repeat the test If repeated tests continue to generate discrepant results contact your distributor or ORFLO Technologies for assistance Alternatively Moxi Z performance can be evaluated using the Electronic Calibration Cassette sold separately which may be helpful in cases where you suspect the calibration check beads have become contam
23. o the Home screen For additional help see the Troubleshooting table page 20 Moxi Z Mini Automated Cell Counter User Manual Page 14 Installing MoxiChart Software on a PC 1 Insert Moxi Z flash drive into a USB port on your computer 4 2 If prompted to run MoxiChart Install click YES and follow the on screen instructions Welcome to the MoxiChart Setup Otherwise open ORFLO DISK folder and Wizard double click on the file MoxiChart_Win_Install exe oe Click Next to continue or Cancel to exit Setup Complete the setup by following the instructions of the MoxiChart Setup Wizard Connecting to Moxi Z via Bluetooth with a PC 1 Press the Bluetooth icon to display the unit s Bluetooth I D Mar 18 20 50 26 System Is Ready v4 To perform a count BlueTooth ID XXXX XXXX XXXX 1 Insert cassette into MoxiZ Model BlueMod P25 G2 2 Pipette 7S5uL of sample FCC ID T7V BC06 into the cassette E 216Q BC06 3 Touch screen anywhere to start See Moxi Z user manual for PC MAC setup information Moxi Z Mini Automated Cell Counter User Manual Page 15 2 Run MoxiChart application and click on Bluetooth icon in upper right corner rad Amos Luc le oe Fe ee es e OR ee ee ar Los RE asrga T m Lier eis Pol Pois a Oe ie l Pi ieee el er ee hh Bij P Lu pee mass pur apa brib ar eed eee ri co Se re colles ss BRIE So 3 Ifthe screen to the right is displayed m simply c
24. oncentration range of the cassette being used n Type M 500 000 cells ml Type S 2 500 000 cells ml then a warning will be provided in the form of yellow background and red High Concentration text in the counts box see arrows Er Dieter i D urve Fit Count Average Size 5 28e 05 Cells ml 14 218 um dia 1 505 pL vol OFA Ry PNP Back Curve Fit Delete ne Gating 2 18um Delet Moxi Z Mini Automated Cell Counter User Manual Page 10 Retrieving and Deleting Data 1 To open a saved test press the Histogram icon on the Home screen 2 Icons for up to nine saved histograms will be displayed on the screen Press the appropriate icon for the test of interest or press the Page Up or Page Down icon to view more test results 3 Each histogram will be opened in either the Curve Fit Count mode or Gated Count mode depending on how it was saved In Gated Count mode gating markers can be positioned as desired by sliding each blue gating marker independently Auto Gate by touching on the desired peak Toggle between the Gated Count and Curve Fit Count modes by pressing the button indicated by the red arrow 4 Use the arrows to the right of the histogram to change the vertical scale of the histogram The vertical scale can also be changed by vertically swiping a finger on the display up to increase or down to decrease Moxi Z Mini Automated Cell Counter User Manual Mar 18 20 50 26 19 System Is Ready
25. ou le taux d humidit est lev comme dans une serre ou un incubateur Se reporter la section sur les sp cifications en mati re de conditions environnementales d exploitation e Ne pas toucher au cable USB ou l adaptateur de charge USB les mains mouill es e Afin d viter tout risque de choc lectrique choisir la configuration de prise appropri e et s assurer que le cable USB et l adaptateur de charge USB sont bien branches dans une prise de courant CA mise a la terre S assurer que la connexion entre le cable USB et l instrument est bien tablie e Toujours s assurer que la tension d entr e du bloc d alimentation correspond la tension disponible dans votre lieu e Ne pas utiliser avec des liquides inflammables ou explosifs e Ne pas immerger le corps de l instrument dans du liquide ou permettre a du liquide de p n trer dans l instrument ATTENTION e Ne pas exposer l instrument des vibrations Les vibrations peuvent causer le dysfonctionnement de l instrument ou des dommages celui ci e Ne pas autoclaver ou exposer des temp ratures lev es e Utiliser uniquement les accessoires autoris s adaptateurs de courant universels c ble USB Si l instrument est chapp et bris d brancher le cable USB et communiquer avec Orflo Technologies Ne pas tenter de d sassembler l instrument Moxi Z Mini Automated Cell Counter User Manual Page 3 Moxi Z Mini Automated Cell Counter Kit The Moxi Z Mini Auto
26. ows Explorer for PC s or Finder for Mac Note Upon power up the Moxi Z will display a notification on the home screen that it is Exporting files to disk see image The Moxi Z disk will be mounted to appear as a drive on the computer following completion of this file export process System Is Ready lt porting files to disk MORGEL 5 Open the Moxi Z drive folder and copy the files to your computer drag and drop or copy and paste Moxi Z Mini Automated Cell Counter User Manual Page 17 Installing MoxiChart Software on a Mac 1 Insert Moxi Z flash drive into a USB port on your computer L A 2 Open the folder for the USB drive 3 Double click on the on the file MoxiChartinstaller dmg 4 Drag and drop MoxiChart into your applications 7 mas folder GS 2 X 5 From the applications folder select MoxiChart to launch the application Connecting to Moxi Z via Bluetooth with a Mac The procedure for connecting Moxi Z via Bluetooth with a Mac is the same as that for a PC See the section entitled Connecting to Moxi Z via Bluetooth with a PC on pages 15 16 Instrument Firmware and Software Upgrades This User Manual describes OS version 4 0 of the Moxi Z Automated Cell Counter firmware The Moxi Z firmware and software may be updated periodically For information on the most up to date firmware and software contact ORFLO Technologies at www orflo com Register your instrument
27. pe S Sterile Cassette Dispenser USB Cable and Power Adapter US version Cat No Quantity Pack MXZ001 1 MXZ002 1 MXZ003 1 MXC001 25 pk 50 tests MXC001 25 25 x 25 pk 1250 tests MXC001 50 50 x 25 pk 2500 tests MXC001 75 75 x 25 pk 3750 tests MXC001 100 100 x 25 pk 5000 tests MXC002 25 pk 50 tests MXC002 25 25 x 25 pk 1250 tests MXC002 50 50 x 25 pk 2500 tests MXC002 75 75 x 25 pk 3750 tests MXC002 100 100 x 25 pk 5000 tests MXC003 25 pk 50 tests MXC003 100 100 x 25 pk 5000 tests MXCO004 25 pk 50 tests MXC004 100 100 x 25 pk 5000 tests MXA001 1 MXA002 1 Moxi Z Mini Automated Cell Counter User Manual Page 28 USB Cable and Power Adapter MXA003 1 EU version Electronic Calibration Cassette MXA004 1 Calibration Check Bead Kit 5 mL MXA005 1 ORFLO Diluent 100 ml MXA006 1 USB Power Cable MXA007 1 Technical Service For technical service contact ORFLO Technologies at 855 TRY MOXI 855 879 6694 or email us at info orflo com Warranty ORFLO Technologies Orflo warrants that its products will meet their applicable published specifications when used in accordance with their applicable instructions for a period of one year ORFLO MAKES NO OTHER WARRANTY EXPRESS OR IMPLIED THERE IS NO WARRANTY OR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The warranty provided herein and the data specifications and descriptions of Orflo products appearing in Orflo s product
28. rating Environmental Conditions Temperature Maximum Relative Humidity 15 30 C 20 80 non condensing Moxi Z Mini Automated Cell Counter User Manual Page 26 Moxi Z Cassette Specifications Type M and Type S Material Polyester Aspiration Volume 75 uL Dimensions Length 3 3 in 8 4 cm Width 1 2 in 3 0 cm Height 0 035 in 0 90 mm Weight 0 1 oz 2 3 g Moxi Z Mini Automated Cell Counter User Manual Page 27 Ordering Information This section lists catalog numbers for the Moxi Z Automated Cell Counter and related products You can purchase Orflo products through a regional distributor or on line at www orflo com See the Technical Assistance section for information about contacting Orflo Product Description Moxi Z Mini Automated Cell Counter Kit U S Version includes Cell Counter Cassette Dispenser USB Cable USB Power Adapter Calibration Beads Cassettes 25 pk and flash drive with software and manual Moxi Z Mini Automated Cell Counter Kit E U Version includes Cell Counter Cassette Dispenser USB Cable USB Power Adapter Calibration Beads Cassettes 25 pk and flash drive with software and manual Moxi Z Mini Automated Cell Counter Kit International Version includes Cell Counter Cassette Dispenser USB cable Calibration Beads Cassettes 25 pk and flash drive with software and manual Cassettes Type M Cassettes Type S Cassettes Type M Sterile Cassettes Ty
29. s a 3 7 V lithium ion battery which can be charged for approximately 500 cycles The battery may be charged at any time in the discharge cycle and can be charged continuously without damage using a PC Mac or the power adapter Refer to the Installation section for information on how to charge the battery CAUTION To prevent battery damage use ONLY the specified power adapter Cat No MXA002 or MXA003 or the USB port of a computer MISE EN GARDE Pour viter d endommager la pile utiliser UNIQUEMENT l adaptateur sp cifi R f n MXA002 ou MXA003 ou le port USB d un ordinateur The battery life is about 2 to 5 years depending on use Lithium ion batteries discharge even if they are notin use To prevent battery damage from self discharge charge the battery at least once every two months Cleaning Sanitizing The Moxi Z Cell Counter is NOT autoclavable Extreme heat will damage the battery touch screen display and other electronic components Moxi Z Mini Automated Cell Counter User Manual Page 24 The external surfaces of the Moxi Z Cell Counter body and touch screen display can be sanitized by wiping with a soft nonabrasive cloth moistened with 70 isopropyl alcohol IPA or 70 ethanol Do not clean the instrument with any more aggressive solutions CAUTION When sanitizing make certain that no liquid enters any internal cavities of the instrument MISE EN GARDE Lors de la d contamination s assurer qu aucun l
30. te Moxi Z Mini Automated Cell Counter User Manual Page 20 a Moxi Apps Mar 18 20 50 26 Mar 22 11 30 41 T O System Is Ready Moxi Z applications can be entered from touching the Apps icon on the home screen Included applications i To perform a count IN verson 4 0 are lal cassette into MoxiZ 2 Pipette SuL of sample into the cassette 3 Touch screen anywhere e Clock to start PONN e Programmable timer with sound notification ORFLO MORGEL e Restore restores unit to factory settings e Loader Puts unit is Firmware OS loader mode Mar 22 11 51 58 e Off powers the unit off Mar 22 11 51 14 Q Hours Minutes Seconds Close Reset Recall Start Stop Moxi Z Mini Automated Cell Counter User Manual Page 21 Troubleshooting Symptom Corrective Action Questionable Concentration of cell Make sure concentration of cell sample is within concentration sample is too high or too recommended guidelines Refer to General low Guidelines section Use a diluent that is compatible with cells being Wrong diluent counted ORFLO Diluent Cat No MXA006 or equivalent Ensure the cells are in a single cell suspension Break Cell clumping clumps by pipetting up and down with a standard pipette ORFLO recommends Accutase or equivalent Questionable cell diameter Use a diluent that is compatible with cells being Wrong diluent counted ORFLO Diluent Cat No MXA006 or equivalent Ensure the c
31. tte Fao sale 3 000 to 500 000 cells mL Type S 3 000 to PS dt 2 500 000 cells ml A L ORFLO For Type M cassettes a dilution of 1 5 to 1 20 is recommended for most mammalian cell lines but the appropriate dilution will depend on cell type and seeding density Type S cassettes will typically require no to 1 2 dilution depending on cell type and seeding density The volume required for an accurate count is approximately 75 uL Turn the Cell Counter on by pressing the power button and the Home screen will be displayed Press the tray down and insert a Cassette into the Moxi Z The A Pipette 75uL Sample screen will be displayed Moxi Z Mini Automated Cell Counter User Manual Page 7 4 Pipette a 75 uL sample into the fill port of the cassette either test 1 y or test 2 depending on which end of the cassette was inserted into the instrument 5 For counting most mammalian cells touch the screen anywhere to start For counting very small particles lt 8 um in diameter the Moxi Z can be run in Small Particle Mode SPM In this mode Moxi Z sets the diameter scale to 2 to 10 um as the default and performs the count using optimized parameters for the detection of small cells Press the Small Particle Mode lt 8 um button to initiate the test and run in this mode 6 The Moxi Z will begin the test and the histogram count results will be complete in approximately 8 seconds Type M cassette or 15 seconds Type S
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