SENTRON Hot-Line probe series User manual
Contents
1. CI solution with a temperature of 20 C for 1 2 hour This cold KCl dip will regenerate the reference system and the diaphragm 7 Specifications For the LanceFET and LanceFET with handle 5 C to 60 C Reference compartment saturated KCl gel non refillable W Storage You can store the probes dry or for some days in KCI solution If stored in a KCI solution the value of the pH measurement can be influenced by the KCI To prevent this and to have a good measurement we advise you to clean the probe after the storage for some minutes at least 5 minutes in demiwater of 25 C2. Distributor Topac Inc 231 CJC Highway Cohasset MA 02025 Tel 781 740 8778 Fax 781 740 8779 www topac com email sales topac com SENTRON Hot Line probe series User manual Manual Hot Line probe SENTRON reference number E 7500179 Version 2 01 May 2003 1 Introduction The SENTRON Hot Line probes series are a series of probes for use in general purposes but also in demanding applications where elavated sample temperatures up to 105 C with the exception of the LanceFET and LanceFET with handle or contact with more aggresive chemicals are to be expected Within the Hot Line series there are three different probe tip types available CupFET for general purpose use and for very small samplevolumes LanceFET for application in semi solids fruit meat fish soil etc LanceFET with handle for LanceFET applications where your hands might get slippery and you might loose your grip on the barrel when applying force to insert the probe in the sample or cold environments where gloves are worn The SENTRON Stream Line probes specially designed for low conductivity applications or highly contaminating samples this probe features a refillable reference liquid compartment with constant reference liquid outlet through a large area porous PTFE diaphragm The Stream Line probes are equally resistant to temperatures and chemicals as the SENTRON Hot Line probes Cleaning Put the probe in soapy water with a constant tem
3. ent is completed and prior to storage Do not use the probe outside the specified temperature range as this might result in probe failure or irreversable damage to the probe Samples must be aqueous solutions or semi solids and compatible with the probes wetted materials If information is required regarding the chemical resistance of the probe please refer to your SENTRON meter manual or contact your local dealer 4 Daily use Remove and save the protection tube from the probe prior to use Reference gel may be observed as a viscous material on the tip of the probe Some gel seepage from a new probe is normal and will not effect the longivity or performance of the probe Prior to daily use scrub the probe tip with a soft bristled toothbrush and water to remove possible residues Use some mild detergent if required Rinse with deionized or distilled water Soak the probe in pH 7 00 buffer or pH 4 00 buffer with the meter ON in any mode prior to calibration for at least 10 minutes If the probe has not been used for two or more days and stored dry clean as above place in saturated KCI solution See Tip 1 for 10 to 15 minutes then in pH 7 00 or pH 4 00 buffer as described above For maximum stability and accuracy perform the calibration sequence twice To store the probe clean it thoroughly with water and a soft bristled toothbrush again a mild detergent may be used and rinse with deionized or distilled water Replace the pro
4. perature of 60 C for minimal 5 minutes Then start cleaning the probe with the toothbrush After this the probe must be placed in a KCl solution with a temperature of 20 C for 2 hour This cold KCl dip will regenerate the reference system and the diaphragm 3 Safety and care The pH sensitive element in a SENTRON probe is an ISFET semi conductor This sensing element needs to be driven by an electronic circuit that is built into the SENTRON pH meters This probe can only perform up to its specification when used in combination with one of the SENTRON pH meters Any other combination might cause loss of performance and irreversable damage to both probe and meter The fact that SENTRON probes are ruggedly build to last even in harsh conditions does not mean that the probe needs no maintenance at all Read the operating tips well to ensure lasting satisfaction Tris buffers and samples containing proteins should be read quickly and the probe should be rinsed thoroughly with deionized water between samples When testing is complete clean with water and a laboratory detergent and rinse with deionized or distilled water Avoid prolonged immersion in samples containing Tris or proteins Avoid prolonged immersion in samples expected to have pH values at the ends of the specified pH range or temperature Range When unavoidable rinse with ample water in between samples Rinse with neutralizing agents and destilled water when the measurem
5. robe Tip 4 When testing in direct sunlight or on a bright reflecting surface please use brown opaque or shielded sample containers Very bright light might influence the performance of the sensor Tip 5 Buffer handling pH 7 00 buffers phosphate based and pH 4 00 buffers biphtalate based are less susceptible to carbondioxide contamination than pH 10 00 buffers borax or carbonate based When slope errors occur it usually indicates a failing probe or a contaminated buffer If seen when using pH 10 00 buffer try calibrating with pH 7 00 and pH 4 00 buffer If a good slope is achieved try a new bottle or different lot pH 10 00 buffer Fresh buffers are available at Sentron ask your local dealer Tip 6 When using ConeFET probes with semi solids insert probe to desired depth then rotate left and right several times and tilt to ensure sample contact Tip 7 To avoid scratches on the chip and or epoxy it is advised only to use the toothbrush after cleaning procedure in the soapy water or after rinsing the probe in water Most scratches cause when there are hard pieces in the sample and on the ISFET Together with the toothbrush these hard pieces can occur scratches on the surface of the chip and damage the epoxy around the chip which can give bad readings Put the probe in soapy water with a constant temperature of 60 C for minimal 5 minutes Then start cleaning the probe with the toothbrush After this the probe must be placed in a K
6. tective tube When doing so slide the screw cap onto the probe body followed by the sealing O ring Slide cap and ring onto the rim of the protective tube press slightly and only then tighten the screw cap Leaving a drop of deiononized or distilled water in the protective tube provides a humid environment avoids dehydration of the reference electrode and therefore allows for faster start up times Note When used in samples containing small hard particles e g pigments titaniumoxides silicates etc first rinse the probe with ample water before using the brush 5 Operating tips Tip 1 To prepare saturated KCI potassium chloride solution Add KCl granules to distilled water untill no more KCI will dissolve Adding 38 grams of KCI to 100 ml water is sufficient Let this stand for at least two hours and decant the clear solution Now you have saturated KCI Tip 2 To ensure correct measurement values samples or buffers need to be mixed well This may be done by a magnetic stirrer or by stirring with the probe for at least 5 seconds Stop stirring and record results when the read out is stable Tip 3 Proteins fats and oils may be removed by scrubbing in a solution of Terg A Zyme Alconox company a pepsin solution or a similar product Afterwards rinse thouroughly with deionized or distilled water Cleaning agents are available at Sentron ask your local dealer Do not use hydrofluoricacid acetone MEK or similar agents to clean the p
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