Compact Dry TC Nissui For Total Viable Count
Contents
1. Compact Dry Nissui TC For Total Viable Count gt Most colonies grown on the TC are red gt Count all colonies grown on the TC gt Incubate for 48 hours at 35 C Main Component Non selective medium and redox indicator of TTC 2 3 5 Triphenyl Tetrazolium Chloride Certificates AOAC PTM MicroVal Storage Keep at room temperature 1 C 30 C Shelf life 18 months after manufacturing Package Compact Dry Nissui TC 40 plates Code 06740 Compact Dry Nissui TC 240 plates Code 06 741 Compact Dry is manufactured based on Nissui s original patented technology o gt NISSUI PHARMACEUTICAL CO LTD 200908TC E01 MN amp Compact Dry Sample os Mey Preparation Preparation 9 Dilution Water Preparation Butterfield s phosphate buffered diluent 34 g of KH PO and 500 mi of distilled water Adjust pH to 7 2 with 1 N NaOH Autoclave 15 min at 121 C 1 10 Dilution Preparation l Pe ll 4 z Analyze 50 g from KO E each sub sample mB Add 450 ml of Butterfield s phosphate buffered diluent Homogenize ina stomacher for 2 min 1 10 Dilution Further Dilutions if necessary 1 100 Dilution Preparation Take out a set of 4 plates pro Or take 1 3 plates you need and keep the pouch sealed to avoid a quality loss from light and moisture lt n SSN d wy Take 1 ml of the 1 10 dilution Add it to 9 ml of Butter2. f colony is amp SUSIE gioun Hus Dees calculated by multiplying the average Ex Lighting from the front number by 20 Viable Count 149 colonies In case that numerous colonies are observed a sample extraction should be diluted appropriately and be inoculated again Ex Lighting from the back Lighting from the front e After opening the aluminum bag unused plates should be kept in the folded and sealed bag to avoid light and moisture e Used plates must be sterilized by autoclaving and disposed according to a local waste management law e In case of foodstuffs containing their own enzymes there are cases where a whole medium of Compact Dry is stained e The pictures in this manual has colonies of E coli There may be subtle differences if other strains grow e Because of the lighting there may be subtle differences in vision between in the pictures and in actual tests Further Information Customer Support Section TEL 81 3 5846 5711 E mail customer nissui pharm co jp NISSUI PHARMACEUTICAL CO LTD
3. field s phosphate buffered diluent ee Mix well Write appropriate information on a gt 1 100 Dilution memorandum section NISSUI PHARMACEUTICAL CO LTD 200908TC E02 MN inoculation ess Weveveads ERRETES ET ss AVANS Miage CERTEN ee f VAS LiL a a f Ti y Fee Turn over the plate lidded put them in an incubator Incubate 48 hours at 35 C for Compact Dry TC Interpretation amp sadid ili From the euteiches of the plate count the number of colored colonies appeared in the medium Lighting and using a loupe are useful to count colonies easier Inoculate the 1 ml sample to the middle of a dry sheet Preliminary Advice For user s convenience Sample Preparation section on the page 2 of this user s manual is made with reference to FDA s BAM Bacteriological Analytical Manual Depending on user s needs other official methods can be also used Put the lid again NISSUI PHARMACEUTICAL CO LTD 200908TC E03 MN Counting Examples amp Precautions Print this page without margins using A4 210 x 297 mm paper the pictures are printed out as the same as the actual Compact Dry 77 x 58 x 7 mm Viable Count 25 colonies In case of more than 300 colonies the number of colony should be estimated The average number of colony in the grid 1 cm is counted and then the total number o
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