User Manual
Contents
1. and O ring Clean and grease O ring and piston clean the tip cone Grease accordingly O ring not correctly positioned or damaged Change the O ring 16 Trouble Possible cause Solution Incorrect operation Follow instruction carefully Calibration altered or unsuitable for the Recalibrate according to instructions liquid Instrument Send for service damaged Piston Clean and grease O Push contaminated ring and piston clean button the tip cone jammed Penetration of Clean and grease O or moves solvent vapors ring and piston clean erratically the tip cone Pipette blocked Liquid has Clean and grease O aspirated penetrated tip cone ring and piston clean volume and dried the tip cone too small 17 Trouble Possible cause Solution Tip ejector jammed or moves erratically Tip cone and or ejector collar contaminated Clean the tip cone and the ejector collar 18 Page intentionally left blank 19 Choice Quality Affordability info labco online com www labco online com Distributor Details2. ready for use Otherwise check both your accuracy and when necessary proceed to recalibration procedure 11 Recalibration procedure 1 Place the calibration tool into the holes of the calibration adjustment lock under the thumb button Fig 7 2 Turn the adjustment lock anticlockwise Fig 7 to decrease and clockwise to increase the volume 3 Repeat performance test Checking calibration procedure from step 1 until the pipetting results are correct 12 9 Maintenance To maintain the best results from your pipette each unit should be checked every day for cleanliness Particular attention should be paid to the tip cone s The pipettes have been designed for easy in house service However we also provide complete repair and calibration service including a service report and performance certificate s Please return your pipette to your local representative for repair or recalibration Before returning please make sure that it is free from all contamination Please advise our Service Representative of any hazardous materials which may have been used with your pipette Note Check the performance of your pipette regularly e g every 3 months and always after in house service or maintenance Cleaning your pipette To clean your Pipettor use ethanol and a soft cloth or lint free tissue It is recommended to clean the tip cone regularly 13 In house maintenance 1 Hold down the tip ejector 2 Place t
3. workmanship Should it fail to function in any period of time please contact your local representative immediately The warranty will not cover defects caused by normal wear or by using the pipette against the instructions gives in this manual Each pipette is tested before shipping by the manufacturer The Quality Assurance Procedure is your guarantee that the pipette you have purchased is ready for use All pipettes have been quality tested according to 1508655 01 12650 The quality control according to 1508655 1 12650 involves gravimetric testing of each pipette with distilled water quality 3 DIN ISO 3696 at 22 C using the manufacturer s original tips Delivery This unit is supplied with 1 x main unit Calibration tool Tube of grease User manual Pipette holder Tips amp Quality control certificate Adjustable volume pipettes Volume Range Increment Tips 0 5 10 0 1 10 2 20 0 5 ul 200 300 10 100ul 1ul 200 300 350ul 20 200ul 1ul 200 300 350ul 100 1000ul 1ul 1000 1000 5000uI 50ul 5m 2 10ml 0 1ml 10ml Volume Range Increment Tips 8 ch 0 5 10 0 1 10ul 8 ch 5 50ul 0 5 200 300 350ul 8 ch 50 300ul Sul 350ul 12 ch 0 5 10ul O 1pl 10 12 ch 5 50 0 5ul 200 300 350ul 12 ch 30 300 Sul 350ul 2 Installing the pipette holder For convenience and safety always keep the pipette vertically on its own holder when not in u
4. Pipettor User Manual Product Code Description Single Chanel Pipettor Variable 550 002 055 Volume 0 5 to 10ul 550 002 060 2 to 20ul 550 002 065 10 to 100ul 550 002 070 20 to 200ul 550 002 075 100 to 100ul 550 002 080 1 to 5ml 550 002 085 2 to 10ml Pipettor 8 Chanel 550 002 110 Volume 0 5 to 10ul 550 002 120 5 to 50ul 550 002 130 50 to 300ul Pipettor 12 Chanel 550 002 160 Volume 0 5 to 10ul 550 002 170 5 to 50ul 550 002 180 50 to 300ul Please read the User Manual carefully before use and follow all operating and safety instructions Technical specifications and outline are subject to change without prior notice Version 1 1 info labco online com www labco online com Contents Introduction Warranty Delivery Adjustable volume pipettes Installing the pipette holder Pipette components Pipette operation Pipetting Techniques Pipetting recommendations Storage Performance test and recalibration Oo ON DH A MN Maintenance 10 Trouble shooting N DU A 9 e e o PPR Introduction Your new hand held pipette is a general purpose pipette for the accurate and precise sampling and dispensing of liquid volumes The pipettes operate on the air displacement principle and disposable tips Warranty The pipettes are warranted for one year against defects in materials and
5. everse pipetting The reverse technique is suitable for dispensing liquids that have a tendency to foam or have a high viscosity This technique is also used for dispensing very small volumes when it is recommended that the tip is first primed with the liquid before pipetting This is achieved by filling and emptying the tip 1 Depress the thumb button all the way to the second stop Fig 6C Place the tip just under the surface of the liquid 2 3mm and smoothly release the thumb button 2 Withdraw the tip from the liquid touching against the edge of the container to remove excess 3 Deliver the preset volume by smoothly depressing thumb button to the first stop Fig 6B Hold the thumb button at the first stop The liquid that remains in the tip should not be included in the delivery 4 The remaining liquid should now be discarded with the tip or delivered back into the container vessel 6 PPipetting recommendations e Hold the pipette vertically when aspirating the liquid and place the only a few millimeters into the liquid e Pre rinse the tip before aspirating the liquid by filling and emptying the tip 5 times This is important especially when dispensing liquids which have a viscosity and density different from water e Always control the push button movements with the thumb to ensure consistency e When pipetting liquids at a temperature different from ambient pre rinse the tip several times before use 7 Storage W
6. ght when a visible sealing ring forms between the tip and the black tip cone Fig 4 Each pipette is fitted with a tip ejector to help eliminate the safety hazards associated with contamination The tip ejector needs to be pressed firmly downwards to ensure proper tip ejection Fig 5 Make sure the tip is disposed of into a suitable waste container 5 Pipetting techniques Forward pipetting Make sure that the tip is firmly attached to the tip cone For best results the thumb button should be operated slowly and smoothly at all times particularly with viscous liquids Hold the pipette vertically during aspiration Make sure that the liquid and container vessel are clean and that the pipette tips and the liquid are at the same temperature 1 2 Depress the thumb button to the first stop Fig 6B Place the tip just under the surface of the liquid 2 3mm and smoothly release the thumb button Carefully withdraw the tip from the liquid touching against the edge of container to remove excess Liquid is dispensed by gently depressing the thumb button to the first stop Fig 6B After a short delay continue to depress the thumb button to the second stop Fig 6C This procedure will empty the tip and ensure accurate delivery Release the thumb button to the ready position Fig 6A If necessary change the tip and continue with pipetting First stop 7 Second stop Fig 6C R
7. he tooth of the opening tool between the tip ejector and the tip ejector collar to release the locking mechanism Fig 8 3 Carefully release the tip ejector and remove the ejector collar 4 Place the wrench end of the opening tool over the tip cone turning it anticlockwise Do not use any other tools Fig 9 The 5 ml tip cone is removed by turning it anticlockwise Do not use any tools Fig 10 5 Wipe the piston the O ring and the tip cone with ethanol and a lint free cloth Note Models up 10 ul have a fixed O ring located inside the tip cone Therefore the O ring cannot be accessed for maintenance 6 Before replacing tip cone it is recommended to grease the piston slightly using the silicone grease provided Note Excessive use of grease may jam the piston 7 After reassembling use the pipette without liquid several times to make sure that he grease is spread evenly 14 Check the pipette calibration 15 10 Trouble shooting Trouble Possible cause Solution Droplets left inside the tip Unsuitable tip Use original tips Non uniform wetting of the plastic Attach new tip Leakage or pipetted volume too small Tip incorrectly attached Attach firmly Unsuitable tip Use original tips Foreign particles between tip and tip cone Clean the tip cone attach new tip Instrument contaminated or insufficient amount of grease on piston
8. hen not in use it is recommended that your pipette is stored in a vertical position 8 Performance test and recalibration Each pipette has been factory tested and certified at 22 C according to 1508655 1 12650 The following table shows the maximum permitted errors Fmax for manufacturers gives in ISO8655 DIN 12650 which further advises each user to establish their own maximum permitted errors Fmax user The Fmax user should not exceed the Fmax by more than 10096 10 Note Pipette specifications are guaranteed only with manufacturer s tips Performance test Checking calibration e Weighing should take place at 20 25 constant to 0 5 C e Avoid drafts 1 Set the desired testing volume of your pipette 2 Carefully fit tip onto the tip cone 3 Pre rinse tip with distilled water by pipetting the selected volume 5 times 4 Carefully aspirate the liquid keeping the pipette vertical 5 Pipette distilled water into a tarred container read the weight in mgs Repeat at least five times and record each result Use an analytical balance with a readability of 0 01 mgs To calculate the volume divide the weight of the water by its density at 20 C 0 9982 This method is based IS08655 DIN12650 6 Calculate the F value by using the following Equation inaccuracy ul 2ximprecision Compare the calculated F value to the corresponding Fmax user If it falls within the specifications the pipette is
9. se When installing the holder please follow the instruction below 1 Clean the shelf surface with ethanol 2 Remove the protective paper from the adhesive tape 3 Install the holder as described in Figure 2A Make sure the holder is pressed against the edge of the shelf 4 Place the pipette onto the holder as shown in Figure 2B 1 me Fig 2A Fig 2B 3 Pipette components Push button rod eo Push button Grip cover Tio Ejector r p EJ Handle Display cover Push button rod Push button i eo Tip Ejector Grip cover Tip Ejector collar Handle Display cover Tip cone housing Tip ejector bar Tip cone Hard tip cone 4 Pipette operation Volume setting The volume of the pipette is clearly shown through the handle grip window The delivery volume is set by turning the thumb button clockwise or anticlockwise Fig 3 When setting the volume please make sure that e desired delivery volume clicks into place e The digits are completely visible in the display window e selected volume is within the pipette s specified range Using excessive force to turn the push button outside the range may jam the mechanism and damage the pipette Sealing and ejecting tips Before fitting a tip make sure that the pipette tip cone is clean Press the tip on the cone of the pipette firmly to Fig 4 ensure an airtight seal The seal is ti
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