B20 User Manual
Contents
1. 20 7 1 20 7 2 Software Installation 20 7 3 The AssayEditor anne Mae tics 21 7 4 AsSay Editing Adee nares Aad atc 22 7 4 1 25 7 5 Assay Settings ine Ae ne de eee ate 26 7 6 Instrument Settings iniiis ee a eal eee TD eae eee 29 7 7 General desicion een a eee 31 7 8 Glossary Of oh cots Reve ee ee eee eed 32 8 TECHNICAL 33 8 1 Spare ones erat 33 9 APPREND tebe ihe eG tale yay yhoo ed staan 34 9 1 Appendix EU DECLARATION OF 34 9 2 Appendix 11 B20 INSTALLATION 35 9 3 Appendix DECONTAMINATION CERTIFICATE 37 Bee Robotics July 2009 Page 2 Bee Robotics Ltd B20 User Manual Version 1 5 B20 Please read this manual carefully before using the B20 and make it accessible to all users Fail ure to comply with the instructions in this manual will void the manufacturer s warranty and may pose a risk to the user2. Wii py B20 User Manual Product Number BB 20 Instructions for Use Version 1 5 IT IS IMPORTANT TO READ THIS MANUAL BEFORE USING THE B20 Bee Robotics Ltd B20 User Manual Version 1 5 TABLE OF CONTENTS 1 B20 OVERVIEW OF INSTRUMENT reana e aea a a a a aa p aa iatea 3 1 1 Miroducio EAE REA 3 1 2 B20 Layout dete ee et tensed aie ie areal 4 1 3 B20 Specifications teehee hited aah 5 2 WARNINGS AND PRECAUTIONS 6 2 1 Limited Warranty A eia 6 3 UNPACKING AND 7 3 1 UNPACKING 0 7 3 2 Environmental gt 7 3 2 1 7 3 3 DISCONNECTION guidelines 8 3 3 1 8 3 3 2 General rn 8 3 4 Electrical 8 4 CUSING 20 3 9 4 1 Pump Setup and Reagent 9 4 2 Installation Check 24 2 8 Sided 10 4 3 10 4 4 Possible Warning
3. 12 4 4 1 Attaining Set Point Temperature 12 4 4 2 Deviation from Set Point Temperature 12 4 5 Cleaning INStrUCTIONS 12 4 5 1 Cleaning the B20 25 92 12 4 5 2 Cleaning Of B20 TrayS 12 5 TROUBLESHOOTING GUIDE 13 5 1 Basic Troubleshooting Visual Inspection 13 5 1 1 13 5 1 2 13 5 2 coated 14 5 3 Basic Troubleshooting 15 5 4 Running The Instrument With 16 5 5 Instrument Failure Further Assistance 17 5 6 Questions To Ask When A Fault Is Reported 17 6 PREVENTATIVE MAINTENANCE 18 6 1 Three Monthly Maintenance ccccccceeeeeeeeeeeeeneeeeeenaeeeeeenaeeeeseaeeeeeeaeeeeseaeeeesseeeeeeeaaes 18 6 2 Six Monthly amp Yearly Maintenance cccccecceeeceeeeneeeeneeeceeeeseaeeseeeeseaeeseeeeseaeeseieeeeenees 19 7 B20 PROGRAMMABLE SOFTWARE
4. Caernarfon Gwynedd LL55 2BD Phone 44 0 1286 672744 Fax 44 0 1286 678322 Bee Robotics July 2009 Page 36 Bee Robotics Ltd B20 User Manual Version 1 5 9 3 Appendix DECONTAMINATION CERTIFICATE Institute a Make of Instrument Model Number 2 Tick This equipment has not been used in an invasive procedure or been in contact with blood other body fluids or pathological samples It has been cleaned in preparation for inspection ser vice or repair This equipment has been cleaned and decontaminated The decontamination method is outlined below This equipment could not be contaminated The nature of risk and safety precautions to be adopted are as follows Signed _______ __ Date Position gt o o ooo Full Official Address Telephone No Bee Robotics July 2009 Page 37
5. EMPTY gt lt EMPTY gt lt EMPTY gt lt EMPTY gt lt EMPTY gt lt EMPTY gt lt EMPTY gt wo Fig 22 The Assay Upload Window N oO Upload progress m Upload Current instruments support the storage of up to 12 individual assays depending on the software version of the Instrument itself The Assay Upload window will appear displaying the names of the currently stored assays Unused slots are named lt EMPTY gt Select a slot and press the Up load button Note if the slot is already occupied the existing assay will be discarded The assay will now start uploading wait for this to complete The on screen progress bar and LCD panel on the Instrument will confirm progress Once completed the Instrument requires turning off and back on At this stage the assay can be run upon the Instrument by pressing the Start button and refinements made as required by repeated editing and uploading Bee Robotics July 2009 Page 25 Bee Robotics Ltd B20 User Manual Version 1 5 7 5 Assay Settings A detailed introduction to the Assay Settings tool will be given in this section This tool allows a user to develop and tune a variety of settings that used within the Assay Editor Note The difference between Assay Settings and Instrument Settings should be understood When you change the values in Assay Settings then these values wi
6. an emergency Do not place the instrument too close to any object that might impair emergency disconnection in an emergency particularly any object to the right or rear of the instrument 3 3 1 Ventilation Do not place the instrument in line with a direct draft such as an air vent Otherwise there are no specific ventilation requirements for the instrument Allow a space of 100mm between the instru ment and any wall or other instruments 3 3 2 General The B20 is intended for indoor use only and should not be subjected to unduly large variations in temperature and humidity This instrument was designed for use in a typical laboratory environ ment see 1 3 Specifications Keep free of dust harsh solvents and acidic vapours Vibration and harsh sunlight must be avoided to ensure correct results The B20 should always be placed on a flat stable surface Storage conditions from 5 C to 50 NOTES instrument is to be relocated with the laboratory it is important to follow the Unpacking and installation procedures outlined in the above paragraphs 3 4 Electrical Power It is essential that U S Models are used in the U S and E U models are used in Europe Check the information on the side of the instrument carefully Leave to stand for 3 hours before applying power to avoid problems that might arise from conden sation In an emergency immediately turn the power off and unplug from power source REPLACE FUSES WITH E
7. perform step 4 described above then either there is a fault with the motor the associated cable the sensor or with the circuit board e instrument fails to perform step 5 described above then either there is fault with the motor the associated cable the sensor or with the circuit board If however the instrument initialises correctly then fill the reagent bottles with water and run the instrument Bee Robotics July 2009 Page 14 Bee Robotics Ltd B20 User Manual Version 1 5 5 3 Basic Troubleshooting Flowchart If you are experiencing problems with your instrument it is worth spending a few minutes perform ing a visual inspection to check for any obvious signs of damage The instrument will always perform the same sequence of events each time it is switched on The aspirating probes will move upwards followed by the dispensing arm moving left to the HOME position followed by the tray support table rocking a couple of times Follow the guide below to help determine where the problem might lie Fig 13 Troubleshooting Flowchart NO Is the Green light Power indicator ON If not check mains fuses and NO or mains cable Replace fuses with correct rating and type if found to be blown Determine which reagent does not prime If the corresponding pump 1 is turning th
8. screws holding the linear slide in place are intact and secure Ensure that the stepper motor is secure and that the HOME sensor tag on the linear slide moves freely in and out of the opto sensor on the arm s circuit board For the arm ensure that the screws holding the linear slide are secure and that the belt is taught For the rocking mechanism ensure that tray support plate moves freely 5 1 2 Fluidics Fig 11 Bottles amp Tubing Check integrity of tubing from the pumps to the bottles Pay particular attention to the tubing connectors at the top of the bottle lids Any damage to the connectors will result in poor or no dispensing If the tubing has become blocked or partially blocked this will also result in poor or no dispensing Tubing Connectors Bee Robotics July 2009 Page 13 Bee Robotics Ltd B20 User Manual Version 1 5 e Ensure that the waste tubing from the aspirating pump and from the waste wash trough is intact and not blocked in any way Ensure that the peristaltic pump clips are correctly posi tioned If the aspirating tubing is blocked the tray will not empty and will result in a spillage and if the waste tubing is blocked the wash trough may not empty e Ensure that the tray locates properly into the clamps on the tray support plate Fig 12 B20 Tray amp Tray Clamps e no problems are encountered during visual inspection the instrument be switched If problems
9. 2 Heater Mat Assembly 110V BHEATM240 002 Heater Mat Assembly 240V Contact your local representative or e mail enquiries beerobotics com to order any spare parts Bee Robotics July 2009 Page 33 Bee Robotics Ltd B20 User Manual Version 1 5 9 APPENDIX 9 1 Appendix EU DECLARATION OF CONFORMITY This is to certify that the B20 Manufactured by Bee Robotics Ltd 32 33 Cibyn Caernarfon Gwynedd Wales UK LL55 2BD Tel 44 0 1286 672 744 Fax 44 0 1286 678 322 The EU Directives covered by this Declaration Conforms with the protection requirement of Council Directive 89 336 EEC relating to Electro magnetic Compatibility by the application of Application of the following EMC standards BS EN 61326 1998 Electromagnetic Compatibility Electrical Equipment for measurement Con trol and Laboratory use Emission and Immunity standard The basis on which Immunity standards are being Declared IEC1000 4 2 Electrostatic Discharge IEC1000 4 3 Radio Frequency Electromagnetic Field IEC1000 4 4 Electrical Fast Transients We are NOT currently declaring compliance with the Low Voltage Directive 72 23 EC The CE mark was first applied in 2001 ATTENTION The attention of the specifier purchaser installer or user is drawn to special measures and limita tions to use which must be observed when the product is taken into service to maintain compli ance with the above directives Details of these speci
10. A Volume of reagent dispensed 0 25 4 0 ml Dispensing accuracy 10 Environmental Operation 15 30 C Operating Temperature Range 18 24 C Storage 10 to 70 C Relative humidity Maximum relative humidity 80 for temperature up to 31 C decreasing linearly to 50 relative humidity at 40 C Altitude Up to 2000m Over voltage category Il Method of Disposal Hazardous waste Usage Commercial Pollution Degree 2 Power Supply 100 120V U S Mains supply voltage fluctuations 220 240V European within 10 of normal voltage F ses T rating 10 amps 110volts T rating 5 amps 220 240Volts Non Operator Internal Fuses Main Controller Board J25 to J30 1A Main Controller Board J31 amp J32 2 5A Power Supply Unit 100 250V 3 2 50 60 Hz Heater Mat Thermal Fuse 92 C 10 amps 110 volts 4 7 amps 220 240 volts Physical Dimensions 470mm wide 480mm deep 500mm height Weight 40kg Bee Robotics July 2009 Page 5 Bee Robotics Ltd B20 User Manual Version 1 5 2 WARNINGS AND PRECAUTIONS Please observe the following caution labels located on the 20 Fig 3 Warning Labels on B20 2 1 Limited Warranty The product is warranted for 12 months from date of shipment to the original purchaser only Claims for merchandise damaged in transit must be submitted to the carrier This warranty sha
11. ENT AT LEAST two people are required to move the carton to unpack and to lift the instrument from the carton and position on the bench The instrument weighs 28kg and the whole unopened carton may weigh as much as 75kg 1 Visually inspect the container for transit damage 2 Place the carton in an upright position to open 3 Unpacking the instrument is packed in a re usable container which may be collapsed for storage It is recommended that appropriate gloves be worn for unpacking some compo nents of the container may have sharp edges Remove the outer straps and lift off the main cover Lift the outer shell upwards and place to one side Lift off the protective foil and care fully lift the instrument off the base of the box and place to one side Two people are required to lift the instrument off the base one positioned at the front of the instrument and one person positioned at the back of the instrument At the same time lift one side of the instrument and remove the foam side and gently rest the instrument down repeat this procedure for the other side of the instrument Remove the foam wedge protecting the viewing window and lift the in strument onto an appropriate bench The outer shell can be collapsed flat and placed onto the base of the packaging and the lid replaced over it 4 Inspect for any obvious signs of transit damage Report any damage immediately to your local representative 5 Check that the serial number on t
12. Ensure all users are conversant with the instrument contact Bee Robotics for any further instrument or training requirements if in any doubt before using the instrument Consult User Manual where symbol left is seen on the instrument 1 B20 OVERVIEW OF INSTRUMENT 1 1 Introduction The B20 is designed for use with reverse line blot strip assay and provides full automation for the washing and hybridisation steps The strips are placed into the 20 well tray held on a platform within the B20 see Figures 2 amp 3 page 4 and the whole detection assay is performed within the 20 well tray Fixed to the under side of the platform is a unique heating mat which gives uniform and precise temperature control during the assay A moving arm both aspirates and dispenses reagents simultaneously across the tray The minimum number of samples that can be processed during a run is 2 and the maxi mum is 20 The B20 is fully programmable from an user friendly windows based software where the operator can custom build their assay protocols Once the assay has been programmed using the soft ware the assay parameters can be uploaded from the PC to run from the instrument using the integral keypad Up to 12 different assay protocols can be stored and selected for use via the in tegral keypad A powerful cross flow fan can be selected to help cool the tray after a heating step The B20 also incorporates two built in pre heaters for warming up reagents pr
13. If trays are not washed immediately after use fill trays with water until they can be washed Note These steps are performed by hand and not in the B20 The B20 tray should be cleaned as follows Add 5ml of an appropriate washing solution to each well used of the tray Carefully rock the typing tray back and forth for 30 60 seconds to dissolve any residue Allow to stand for 5 minutes Pour off the washing solution well into sink with running water Repeat steps 1 and 2 Rinse each well in the tray thoroughly with de ionised water Allow tray to air dry The tray is now ready for re use Bee Robotics July 2009 Page 12 Bee Robotics Ltd B20 User Manual Version 1 5 5 TROUBLESHOOTING GUIDE 5 1 Basic Troubleshooting Visual Inspection A good visual inspection of the instrument prior to switching it on can sometimes reveal problems or potential problems Performing these simple checks will help determine the condition of the in strument 5 1 1 Mechanics Fig 10 B20 Arm Open the lid and ensure that the aspirating probes on the arm move upwards and downwards freely by hand Leave the aspirating probes in the up position and move the arm from right to left by hand ensuring that the arm moves freely If any resistance or obstructions are encoun tered during these tests the problem will be a mechanical one Aspirating Probes Dispensing Arm For the aspirating probes ensure that the two
14. N b The instrument will initialise The initialisation sequence of events are i The display will indicate BeeBlot Ready Press Start ii The aspirating needles move up to their HOME position iii The arm will move left to its HOME position The tray mechanism will move into its HOME position v The green LED at the front of the instrument will come on Stat Hold Quit Fig 6 B20 Keypad amp Display 4 3 Initial Run Before using the instrument for analysing samples it is recommended that the following series of instrument checks be undertaken Priming dispensing and aspiration of wash solution Ensure that the power to the instrument is OFF Ensure that the waste tube is routed either into a suitable sink or into a suitable 4 litre empty container One of the waste tubes is gravity fed Ensure the waste container is BELOW THE LEVEL OF THE MACHINE AND THE WASTE TUBES ARE NOT TRAPPED UNDER FEET Switch Power ON and ensure that the instrument initialises properly refer to Installation Check above B20 will have one or more assays preloaded when new It is recommended that a dummy run is set up with water to ensure that the selected assay performs the steps as required Alternatively it is possible to create an assay by using the programmable software provided with the instrument It is necessary to upload the assay into the instrument from your PC al lowing the operator to run the instrum
15. XACTLY THE SAME TYPE AND RATING Bee Robotics July 2009 Page 8 Bee Robotics Ltd B20 User Manual Version 1 5 4 USING THE B20 4 1 Pump Setup and Reagent Tubing Tygon tubing is used throughout the instrument The tubing fittings are luer lock type The B20 has 7 pumps The material in the peristaltic pumps is Pharmed The Pump Layout is as shown ASP is the aspirating pump All the other pumps are reagent dispensing pumps Fig 4 Pump Layout of B20 There are 6 pairs of dispensing needles in the instrument arm Pair 1 is positioned at the front of the arm The instrument dispensing head is organised as follows _____ Aspirating Needles Pair 6 9 Pair 5 Fig 5 Drawing of Pair 4 dispensing layout Pair Pair 2 Sa Pair 1 Each pump has the following typical function Pump Number Needle Set Dispensed Through ASP Aspirates through aspirating needles Pump 1 Dispensed through pair 6 Pump 2 Dispensed through pair 2 Pump 3 Dispensed through pair 3 Pump 4 Dispensed through pair 4 Pump 5 Dispensed through pair 5 Pump 6 Dispensed through pair 1 Bee Robotics July 2009 Page 9 Bee Robotics Ltd B20 User Manual Version 1 5 4 2 Installation Check a Switch instrument O
16. al measures and limitations are contained in the product manual Bee Robotics July 2009 Page 34 Bee Robotics Ltd B20 User Manual 9 2 Appendix Il B20 INSTALLATION CHECKLIST INSTRUMENT SERIAL NUMBER Version 1 5 CUSTOMER NAME ADDRESS TELEPHONE NUMBER FAX NUMBER E MAIL ADDRESS Did the B20 arrive in good condition Yes No If no provide details Were the following accessories present Mains Lead Yes No User Manual Yes No Spare Fuses Yes No Assay Editor CD Yes No Null Modem Cable Yes No Bottle Holders Yes No Bottle Set Yes No 1 x Spare 20 Well Tray optional Yes No If no provide details Were the priming dispensing and aspirating Yes No steps performed correctly Bee Robotics July 2009 Page 35 Bee Robotics Ltd B20 User Manual Version 1 5 If no provide details Did the Watlow Controller switch on Yes No Comments Did the temperature start to rise Yes No Comments Any other comments Representative Name Representative Address Representative Signature Date Customer Signature Date Customer Print Name Position Customer to retain original copy Distributor must send copy to QA Department Bee Robotics Ltd Unit 32 33 Cibyn Industrial Estate
17. are encountered during visual inspection these must be rectified prior to switching the instrument on otherwise further damage may be done to the instrument 5 2 Initialisation The instrument will always perform the same initialisation routine when it is switched on Prior to switching on the instrument move the arm to half way across it s travel and move the aspirating probes half way down When the instrument is switched on the sequence of events should be as follows The green LED at the front of the instrument should be on The display will indicate BeeBlot Ready Press Start The aspirating probes should move upwards to its HOME position The arm will move left to it s HOME position The tray rocking mechanism will move to it s HOME position If the instrument fails to perform any or all of these tasks then there is a fault which needs to be rectified e If the instrument fails to perform sequence 1 described above then either there is a fault with the 24V power supply or the mains fuses have blown e If the instrument fails to perform sequence 2 described above and there is nothing or random characters on the display then either there is a fault with the associated cable the circuit board or the display itself e instrument fails to perform step 3 described above then either there is fault with the motor the associated cable the sensor or with the circuit board e instrument fails to
18. ate priming to reduce the waste of valuable reagents Enter a time in seconds for each pump Valid times range from 0 25 seconds Bee Robotics July 2009 Page 29 Bee Robotics Ltd B20 User Manual Version 1 5 Instrument Settings Cleaning Cycle Setup Cleaning Cycle B Setup Pump Selector Settings m Pump Prime Timings 5 65 65 65 Fig 28 The Pump Primer Win cle OK Apply Settings A number of items appear in this window see Fig 29 The first is the interval the heaters are turned on to raise the temperature of the reagents the so called preheat time prior to an assay actually starting Enter a value in minutes The next item allows the user to select which pump will provide the water for the empty wells Fill ing the empty wells with water is required to maintain the correct thermal characteristics of the tray It is not expected the user will change the default The aspirating time item allows the user to specify the interval of time for a well aspiration The user will want to minimise this time whilst maintaining the complete removal of liquid from the wells The default value is 1 The last option allows you to set which communication port the program uses to connect to the instrument This is usually 1 with a direct RS232 Cable 34 if using an USB adaptor Instrument Settings Cleaning Cycle Setup Cleaning Cycle B S
19. d down Bee Robotics July 2009 Page 15 Bee Robotics Ltd B20 User Manual Version 1 5 5 4 Running The Instrument With Water Each reagent has its own dedicated pump which means it s straightforward to determine which if any of the dispensing lines are blocked SC _____ Aspirating Needles Fig 14 Pump Layout of B20 Lee Pair6 __ Pair 5 es 1 Pair 4 ___ _ Pair 3 Fig 15 Drawing of dispensing layout Pair 2 Pair 1 000000 Perform the cleaning cycle to ensure that the water flows through the appropriate dispens ing needles under the arm Observing the cleaning cycle will ensure that each reagent tubing line and the corresponding pump is functioning correctly If nothing is dispensed through the dispens ing needles and the corresponding pump is turning then there is a blockage in that particular re agent line or the fluid lines have been assembled incorrectly during servicing If the corresponding pump does not turn then the pump or circuit board is faulty An engineer would plug the pump that doesn t turn into another position on the circuit board and go through the priming sequence again If the pump fails to turn then the problem is with the pump If the pump now tur
20. e Open Assay item in the File menu A further window appears and provides a browser to locate the file of an existing assay make a selection and click Open The assay editor will now be displaying your selected assay which can be further refined or uploaded to your Instrument Bee Robotics July 2009 Page 21 Bee Robotics Ltd B20 User Manual Version 1 5 Saving an assay At the point you have finished editing your assay you must save it Select Save Assay from the file menu in the Assay Editor The Save window will appears enter a name for your assay and save it to a convenient location The default folder for saving assays is called assays and is found in the installation folder of the Bee Blot Assay Editor Assay navigation An assay is composed of a number of individual steps and the Assay Editor displays each step on a separate page The editing controls are used for the navigation and editing of steps and ap pear below the horizontal grey bar of the Assay Editor window see Fig 1 The Next Step and Prev Step controls will cycle through the individual steps of the assay a little like leafing through pages of a book where only one page is visible at a time Delete Step and Add Step manage the addition or removal of individual steps within the assay In addition the Assay Editor also displays the current step and the total number of steps just above the navigation buttons Next Step display
21. e pumps tubes and nozzles an Instrument s storage area for an assay The action of warming reagents prior to an assay starting Bee Robotics July 2009 Page 32 Bee Robotics Ltd B20 User Manual Version 1 5 8 TECHNICAL SUPPORT Manufactured By BEE ROBOTICS LIMITED 32 33 Cibyn Caernarfon Wales UK For all technical support and assistance contact your local distributor or Technical Department Bee Robotics Ltd 32 33 Cibyn Caernarfon Gwynedd Wales UK LL55 2BD Tel 44 0 1286 672744 Fax 44 0 1286 678322 Web www beerobotics com E mail enquiries beerobotics com Only by Bee Robotics or associated trained service personnel may repairs and servicing be carried out For further details of instrument or to arrange for training to be given on the instrument contact Bee Robotics at above address 8 1 Spare Parts List Stock No Description BPERIP 002 Peristaltic Pump BW935 002 Watlow Controller BTRAY 020 20 Well Tray BXBELT 002 X Axis Belt BTBELT 002 Table Rocking Belt BCBLESET 002 Internal Cable Set BFUSEUS 002 US Internal Fuse Set BPSU24V 002 24V Power Supply Module BID803001 001 Z Distribution Board BID803020 Main Circuit Board BDISPM 001 Display Module BKPD 001 Keypad Membrane BTUBS 001 Tygon Tubing 1 16 BTUBL 001 Tygon Tubing 3 32 BPERIT 002 Peristaltic Pump Tubing BLUERSET 002 Tubing Luer Connectors Set 110 00
22. en there is a blockage in that particular line Check all luer connectors for damage gt Does the instrument lt initialise correctly YES NO Does the Do the aspirating instrument prime probes move correctly upwards YES YES Switch Off Move mechanism up and down by hand It should be free to move without any obstructions If you experience difficulties there may be a mechanical problem Ensure that the aspirating mechanism is not loose lt _ Does the tray empty correctly Does the arm move to its Home position gt Ensure that the aspirating tubing is attached to the aspirating probes lt Ensure that the aspirating pump is turning Ensure that the waste tubing is not obstructed in any way YES Switch Ensure the aspirating probes are in the up position and move the arm right to left by hand It should be free to move without any obstructions If you experience difficulties ensure the arm is not loose and that there are no loose screws underneath the linear slide Does the tray support table rock gt YES Switch Ensure that the rocking table is not loose Turn the rocking mechanism by hand to ensure that the table moves up an
23. ent from the integral keypad Refer to section 7 of this manual for instructions on creating and uploading assays Fill all reagent bottles with de ionised water Note it is important not to run the instrument without liquids Serious damage to the instrument may occur Place the supplied tray on the table of the instrument TRAY ORIENTATION The tray has been designed in such a way that it can be positioned in one direction only The operator must position the sensor into the tray as prompted by the keypad display prior to starting the assay Always position sensor so that it is in the fully down position prior to starting an assay The sensor must be treated with care at all times Fig 7 Temperature Sensor Press Start on the Keypad select the required Assay and follow the keypad commands Bee Robotics July 2009 Page 10 Bee Robotics Ltd B20 User Manual Version 1 5 Depending on the assay selected there may be an option to select a Cleaning Cycle A B Press Quit on these steps Select the number of samples to be 2 press Start and follow the keypad commands until the run commences Following the first dispensing phase the tray should start to rock amp the Wat low Controller will come on depending on the assay program Ensure that the temperature starts to rise WATLOW Press Quit to switch off the temperature controller and ensure that the display indicates Heater Error and that
24. ep is always a dispense only step with the option to pause with the heater selected and the tray rocking to add Amplicon File Options Help Upload Robotics Ltd Assay name Step 2 2 Step name Unnamed Step Delete Step Add Step gt lt lt Prev Step Next Step gt gt Overall step settings Maintain reagent preheating HeaterA HeaterB Tray temp 500 z degC Redirect waste 7 Dispension phase Dispense only with pause option Dispense only parameters Pause time 0 minutes Reagent name Unnamed Reagent Tray positioning Rocking z Select reagent pump zs Pause for manual dispense m Incubation phase Incubation time minutes 0 Heater Tray positioning Rocking z Fan Fig 20 The Assay Editor displaying the additional dispensing parameters The next item to consider is the name of the regent to be dispensed A text box is provided for this enter the name of the reagent to be dispensed A number of common reagent names are available in the drop down menu of the text box for the users convenience This text will appear on the Instruments LCD during the dispense phase and is limited to 16 characters in length The next item is the tray positioning This specifies the position the tray should adopt during the dis pensing of this step Also enter the volume of regent to be dispensed per well If required the fan ca
25. es Cleaning cycles to be included Cycle A after the assay finishes Cycle B OK Apply Preheating Phases Certain reagents precipitate at ambient temperatures so the Instrument usefully incorporates two heated reagent receptacles Preheating can be included prior to an assay starting Click on the Preheating Phase tab and the Preheating window will appear see Fig 24 To include preheat ing tick either or both of the boxes in this window reagent A is on the left side of the Instrument and reagent B on the right Selecting these tick boxes will turn on the heated reagent receptacles Bee Robotics July 2009 Page 26 Bee Robotics Ltd B20 User Manual Version 1 5 prior to the assay starting The length of time these heaters are on is set in the Instrument Set tings tool see section 7 5 Assay Settings Priming Reagent 5 i Pump Colour Palette Cleaning Phases Prompt Phase Preheating Phase Preheat cycle Fig 24 The Preheating Phase Window Preheat cycles to be included Preheat reagent before the assay starts Preheat reagent OK Apply Prompt Phase User messages can be added to the start of an assay in order to prompt an operator to manually add the chemically sensitive strips reagents or any other manual intervention required prior to the assay starting The start button must be pr
26. essed in order to proceed from a prompt message The user can add a text message to be displayed on the LCD the drop down menu provides com monly used messages The tray can be positioned as required for each instruction To set up the prompting click the Prompt Phase tab the Prompts Editor window will appear The wizard based interface is similar to that of the Assay Editor as can be seen in Fig 25 The Prompts Editor window displays the current instruction and the total number of instructions just above the navigation buttons see Fig 25 To navigate through the steps use the Prev Prompt and Next Prompt buttons If additional instructions are needed the Add Prompt button will add a blank message after the currently displayed instruction Also the deletion of an instruction may be necessary use the Delete Prompt button to perform this Assay Settings x Priming Reagent Save Overide Pump Colour Palette Cleaning Phases Preheating Phase Prompt Phase m Prompt Phase Prompt 1 ofa Fig 25 The Prompt Phase lt lt Prev Next gt gt Delete Add gt Window m Prompt Setup Display text Add reagents 16 chars Tray positioning Tilted OK a Bee Robotics July 2009 Page 27 Bee Robotics Ltd B20 User Manual Version 1 5 Priming This window provides the user with a means to specify individual pump prime times The user will want t
27. etup Pump Selector Pump Primer i Settings Preheat duration 75 mins Fig 29 The Settings Window Water fill pump for empty wells 5 Aspirating time offset 0 z seconds Use com port fi OK Apply Bee Robotics July 2009 Page 30 Bee Robotics Ltd B20 User Manual Version 1 5 Cleaning Cycles The final two tabs correspond to the cleaning cycles of which there are two the tabbed pages are otherwise identical see Fig 30 During a cleaning cycle each pump will be turned on individually for the length of time set in this window The soak time is the interval where the reagents are left in the delivery system Selecting the reagent save tick box will ensure reagent use is minimised by reversing the peristaltic pumps and clearing the lines Cleaning cycles can be included in an as say by selecting them from the Assay Editor Instrument Settings Pump Primer Settings Cleaning Cycle Setup Cleaning Cycle Setup 105 105 105 105 Fig 30 The Cleaning Cycle Set up Window 105 105 Soak time mins fi Include reagent save 7 OK Cancel Apply 7 7 General At the end of the assay the tray will remain level which means that the strips will remain solu tion if performing an overnight run The operator will then be given the choice of using the instru ment to aspirate the tray or to press the Quit ke
28. finalised with an automatic aspiration of the spent reagents Creating a new assay In order to become familiar with the development of custom assays bring up the Assay Editor on screen Initially a user is provided with an example assay select the New Assay item in the File The user will be prompted for an assay name and here a name should be entered up to 16 char acters Subsequently a wizard based editor will appear containing a default assay with a single step File Options Help Robotics Lid Step of 7 Step Unnamed Step lt lt Prev Step Next Step gt gt Overall step settings Delete Step Add Step gt Maintain reagent preheating Heater4 HeaterB Redirect waste 7 Dispension phase Reagent name Unnamed Reagent 7 Trayipasitionng Rocking 1 Select reagent pump 000 Pause for manual dispense m Incubation phase Incubation time 0 minutes Tray positioning Rocking Fan Fig 19 The Default Assay Editor Note the Assay Editor window contains a number of items the main features to draw attention too are the Edit Controls the Overall Step Settings and the Dispension Phase controls Opening an existing assay Alternatively an existing assay can be loaded and refined Again bring up the Assay Editor on screen from the main window Initially a user is provided with an example assay select th
29. he instrument and the delivery note are the same 6 Check the remainder of the contents against the delivery note See the nstallation Checklist at rear of manual for details of accessories that should be present 7 Store packing materials until you are completely satisfied that the instrument was delivered safely and that it is performing to specification 3 2 Environmental conditions 3 2 1 Bench Space At least 500 mm x 500 mm space is required at bench level The lid swings upwards so that when open the height is 1 metre from the instrument base to the top of the lid The instrument should be placed on a bench at least 15cm away from the wall Appropriate power as described in the specification must be available within 1 metre It is preferable to locate the instrument near a suitable laboratory waste outlet Alternately posi tion a waste container of at least 4 litre capacity in close proximity to the instrument One of the waste tubes is gravity fed so ensure the waste container is below the level of the machine Ensure waste pipes are not trapped under the instrument Bee Robotics July 2009 Page 7 Bee Robotics Ltd B20 User Manual Version 1 5 3 3 Disconnection guidelines The mains switch and mains lead connection is to the bottom right hand side as viewed from the front The instrument should be 120mm from any side wall so that there is ample clearance for the mains connector and to facilitate disconnection in
30. ior to being dis pensed into the tray and a solenoid valve for re directing waste All these features can be in cluded into any assay using the programmable software Bee Robotics July 2009 Page 3 Bee Robotics Ltd B20 User Manual Version 1 5 1 2 B20 Layout Fig 1 Front view of B20 Instrument Robotic arm with dispensing and aspirating needles Display and Key pad Watlow Controller One of seven Peri staltic Pumps Two reagent pre heaters Position for re agent containers Fig 2 Rear 34 view of B20 Instrument Serial Label Bee Robotics July 2009 Page 4 Bee Robotics Ltd 1 3 B20 Specifications B20 User Manual Version 1 5 Display unit LCD with 2 rows of 16 digits Keyboard 5 membrane covered switches Number of dispensing heads 6 Cross flow Fan 24v DC Reagent Pre Heaters 2 24v DC 80 C Thermostat Control 92 C thermal fuse Optional Reagent Save Feature Reversible Pumps Peristaltic Pumps 7 on board Power ON Indicator Green LED Capacity of reagent containers 1 500 2 250 4 125ml may differ slightly Computer interface RS232 Test capacity Minimum 2 samples maximum 20 samples Instrument control Interactive keypad Temperature setting Ambient to 55 C Temperature Control Programmable Watlow Controller Temperature Sensor PT100 Class
31. is turn ing and that there are no obvious blockages 3 Clean the tray support table using an alcohol wipe It is important to keep the tray support table clean at all times Fig 16 Tray Support Ta Tray support table ble 5 6 7 9 10 11 12 13 14 15 1 4 Flush the instrument through with 1 Chloros followed by de ionised water This can be done using the optional wash program at the beginning or at the end of the assay see page 12 t Chloros is an irritant and is harmful Bee Robotics July 2009 Page 18 Bee Robotics Ltd B20 User Manual Version 1 5 6 2 Six Monthly amp Yearly Maintenance Depending on the usage of the instrument the schedule below should be carried out on the B20 Every 6 Months Every 12 Months Frequent use Perform all the checks below apart from Perform all of the checks outlined below i e 2 3 runs per week step 5 Heavy use Perform all of the checks outlined below Perform all of the checks outlined below i e 1 2 runs per day If all of the checks below are to be carried out they must be done so by Trained Personnel 1 Carry out the 3 monthly checks outlined in step 6 1 2 With the instrument switched off ensure that the aspirating probes move up and down freely 3 With the instrument switched off ensure that the arm moves freely from left to right Add a drop of light machine oil to X axis linear slide No more than a drop should be ap
32. ll not apply to any product which shall have been altered outside Bee Robotics nor shall it apply to any which have been subjected to misuse or mishandling ALL OTHER WARRANTIES EX PRESSED IMPLIED OR STATUTORY ARE HEREBY SPECIFICALLY EXCLUDED INCLUD ING BUT NOT LIMITED TO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PAR TICULAR PURPOSE Bee Robotics Ltd s maximum liability is limited in all events to the price of the products sold by Bee Robotics INNO EVENT SHALL BEE ROBOTICS BE LIABLE FOR ANY SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES This product may not be repackaged reformulated or resold in any form without the written con sent of Bee Robotics Ltd Bee Robotics Ltd reserves the right to make technical improvements to the B20 and B20 documentation without prior notice as part of a continuous programme of product development As part of the WEEE EU directives this instrument should be returned to the manu facturer for dismantling at the end of its life TRADEMARKS AND PATENTS Trademarks B20 is a trademark of Bee Robotics Ltd UK Tygon and PHARMED are registered trademarks of Norton Performance Plastics Corp Intellectual Property Rights The B20 and any derivatives of this product remains the Intellectual Property of Bee Robotics Ltd Bee Robotics July 2009 Page 6 Bee Robotics Ltd B20 User Manual Version 1 5 3 UNPACKING AND INSTALLATION 3 1 Unpacking CAUTION HEAVY INSTRUM
33. ll be applied to the assay you are currently working on ONLY The values in the Instrument Settings are the default values applied to future assays but NOT necessarily to the assay you are currently working on Starting the Assay Settings Tool To start this tool click on the main menu Options gt Assay Settings A tabbed window will ap pear with each tab corresponding to an aspect on the current Assay or the instrument which is detailed below Cleaning Phases Cleaning is crucial to maintain your Instrument in peak condition A cleaning cycle will pump water or other cleaning agents through the delivery pipes leave it to soak for some time and then re verse pumping direction and scavenge the pipe clear Cleaning can be performed prior to the as say starting or after it completes To specify the cleaning cycles of an assay click on the Cleaning Phases tab a window will ap pear as pictured in Fig 23 Two cleaning cycles are available for selection at either the start or the end of the assay or both The parameters of the Cleaning Cycle are specified within the Instru ments Settings window Assay Settings Oy x Priming Reagent Save Overide Pump Colour Palette hase Preheating Phase Prompt Phase Cleaning Phase Startup Cleaning Cycles Cleaning cycles to be included Cycle A Fig 23 The Cleaning Cycle Win Cycle B dow Final Cleaning Cycl
34. lly 5 minutes will be automatically subtracted from the incubation time Incubation Time the period of time to incubate Tray Positioning the required positioning of the tray during incubation Heater turns on the tray heater for the incubation duration Fan turns on the tray fan for the incubation duration Bee Robotics July 2009 Page 24 Bee Robotics Ltd B20 User Manual Version 1 5 7 4 1 Uploading an assay At the stage the developer is satisfied with the custom assay it can be directly uploaded to the Instrument via the serial connection Firstly attach the supplied cable between the PC and the Instrument This cable is of a null modem type In the Assay Editor press the Upload button You will then be prompted to switch the instrument off check the serial cable and switch the instru ment back on These steps need to be followed accurately for the uploading to work correctly You might see a Connecting window whilst the program is waiting for the instrument to finish initialis ing You must then enter your access key which is unique to each individual instrument The access code is located on the front of your installation CD Key Entry x Enter connection key Fig 21 The Key Entry Window Proceed correct access code will display the Assay Upload window Assay Upload Select destination lt EMPTY gt lt EMPTY gt lt EMPTY gt lt EMPTY gt lt EMPTY gt lt
35. ly uploaded to the Instrument via the serial connection The assay can subsequently be run upon the Instrument and refinements made as required by repeated editing and uploading Further to this the Instrument Settings tools enable a user to refine various instrument system parameters for their particular requirements such as the preheating and aspirating time intervals Cleaning cycles priming features and reagent saving features are also set up here 7 2 Software Installation This section describes how to install and run your Bee Blot Assay Editor software The software is available as either a CD or as a ZIP file Both methods provide an automated installation wizard for your convenience Follow the instructions appropriate for your medium as found below Systems Requirements Checklist Bee Blot Assay Editor will run on virtually all current PCs providing they are Windows based Windows 95 Windows 98 Windows NT Windows 2000 Pro and XP are all supported The only other requirement is one unused serial port on your PC Clearly within a laboratory environment a laptop makes a particularly versatile PC for this task CD Installation Insert the supplied CD into your CD drive and follow the on screen instructions If the CD does not auto run then follow these instructions Open the start menu and click on Run In the pop up text box type D Setup exe where D is the letter of your CD drive and press ok Follow the
36. ment is under warranty or not and if for some reason we need to refer to the instru ment s history file Does the instrument initialise properly Yes or No Yes then the problem is likely to be a heating problem or a fluidics problem Ask the opera tor to run the instrument with water and check the priming sequence to ensure that there are no blockages If there are no blockages the operator should check that the aspirating probes are emptying the tray if the tray is not emptying the tray will overfill No then the problem is likely to be mechanical or electronic Ask the operator to determine exactly at what stage the initialisation fails in order to diagnose the problem Bee Robotics July 2009 Page 17 Bee Robotics Ltd B20 User Manual Version 1 5 6 PREVENTATIVE MAINTENANCE SCHEDULE In order to keep the B20 in good working order it is recommended that the following steps be fol lowed as part of a regular field preventative maintenance program 6 1 Three Monthly Maintenance The following checks must be carried out on a three monthly basis 1 Check that the aspirating tubing is free and unobstructed This be checked visually and by running the instrument with water and ensuring that the wash trough empties dur ing aspiration 2 Check that the dispensing needles underneath the arm are dispensing correctly during priming If any of the needles fail to dispense ensure that the corresponding pump
37. n be turned on for the duration of this phase a tick box is provided for setting this Please note that the tray must be in a rocking position if the heater is on as the Assay Editor does not allow any other configuration The program also disallows the heater and the fan to be switched on at the same time Bee Robotics July 2009 Page 23 Bee Robotics Ltd B20 User Manual Version 1 5 the user requires the Instrument to pause after it has performed an automatic dispense then the pause for manual dispense tick box must be selected Typically this feature will be used to manually add a sample after the first reagent dispense When the assay is running on the Instru ment it will require the user to press the start button on the LCD panel in order to proceed It is possible to have the heater selected for this pause however the tray position will automatically default to rocking if the heater is required to come on at this stage It is imperative that liquid is always added to the well with the sensor prior to the heater turning on If the heater box has been selected then the operator must also select the temperature requirement for that particular step See Fig 20 The final setting is the reagent bottle to be used during this dispense phase A number of circles can be seen in the window see Fig 20 The coloured circles represent the dispensing pumps as follows Top Row from left to right Dispense Pump 1 Dispense Pum
38. ns in this new position then the problem is with the circuit board If the cleaning cycle is performed correctly then start an assay and allow the instrument to dis pense the first reagent into the tray then allow the arm to return to its HOME position Take a look at the dispensing needles underneath the arm No water should be flowing through or continu ously dripping at this stage If water is present then there is a leak in the corresponding tubing or the pump clamp has not clipped into place properly Press quit on the keypad and observe the aspirating needles ensuring that the tray wells are emptying When the next reagent is added you may see some drops forming after the pump has stopped turning this is normal and does not indicate a fault unless there is a continuous flow of drops which would have been observed when the arm was in the HOME position Bee Robotics July 2009 Page 16 Bee Robotics Ltd B20 User Manual Version 1 5 5 5 Instrument Failure Further Assistance If the instrument fails it is often useful to repeat the B20 run with water to see if the fault occurs again To do so fill all reagent bottles with DI water amp proceed with the assay as normal Do not add strips to the tray Check that each step of the assay is being performed correctly 5 6 Questions To Ask When A Fault Is Reported What is the instrument s Serial Number This information is required to determine whether the instru
39. o minimise these times whilst preserving adequate priming to reduce the waste of valuable reagents Enter a time in seconds for each pump Valid times range from 0 25 seconds Reagent Save Override Your Bee Blot Assay Editor includes the feature to automatically save reagents after they have been used in an assay By reversing the direction of the peristaltic delivery pumps valuable re agents are removed from the pipes for reuse Ensure Yes is selected for this item if you require this feature Reagent Bottle Colour Palette This window provides a convenient means to graphically colour code the reagent pumps see Fig 26 To perform this click on a colour in the colour selector and then click on one of the circles These circles represent the physical arrangement of the corresponding reagent bottles of an In strument This colour coding will be used throughout the other tools for the convenience of the user Assay Settings x Cleaning Phases Preheating Phase Prompt Phase Priming Reagent Save Overide Reagent Bottle Colour Palette Fig 26 The Reagent Bottle Colour Palette Window OK Cancel Again the coloured circles represent the dispensing pumps as follows Top Row from left to right Dispense Pump 1 Dispense Pump 3 Dispense Pump 4 Dispense Pump 6 Bottom Row from left to right Dispense Pump 2 Dispense Pump 5 Bee Robotics J
40. on screen instructions Zip File Installation If a CD was not supplied the ZIP file may have been supplied to you as an email attachment Double click on the WinZip file to open it If WinZip does not start then you will need to install this too WinZip is available to download from http www winzip com When the WinZip window ap pears double click on the Setup exe file WinZip should then automatically extract and run the installation follow the on screen instructions Starting the Bee Blot Assay Editor Providing installation was successful you will find a Bee Blot Assay Editor icon in your Windows Start menu Start gt Programs gt BeeBlot Select this icon to start the Bee Blot Assay Editor it is not necessary to have an Instrument attached After the splash screen you will find that the main Bee Blot Assay Editor window will appear see Fig 1 Congratulations your Bee Blot Assay Edi tor is installed Bee Robotics July 2009 Page 20 Bee Robotics Ltd B20 User Manual Version 1 5 7 3 The Assay Editor A detailed introduction on using the Assay Editor will be given in this section This Assay Editor is used to develop custom assays and subsequently upload them to an Instrument As far as the Assay Editor is concerned an assay is a collection of steps where each step represents a single operational cycle An operational cycle would typically include a reagent dispense followed by an incubation period which is
41. p 3 Dispense Pump 4 Dispense Pump 6 Bottom Row from left to right Dispense Pump 2 Dispense Pump 5 Select the appropriate reagent circle and a S will show it has been selected For the users con venience the colours used can be set to match your own physical colour coding see section 7 4 for more information on this Dispense Only when ticked a pause is introduced prior to dispensing Reagent Name the name of the reagent to be dispensed Tray Positioning the tray position for this phase either tilted or rocking Volume the volume of reagent to be dispensed Fan turns on the tray fan during this dispensing phase Pause for Manual dispense a pause is introduced after dispensing Reagent Pump select the reagent bottle to be used during dispensing The Incubation Phase Controls The last programmable phase is incubation Following the completion of a dispensing phase the incubation phase will begin This simple phase is for the purpose of progressing the chemical re actions acting on the strips within the wells The incubation phase for the currently displayed step is specified with several controls Firstly enter or select the time required for incubation and the tray position Additionally add heating or cooling by selecting the heater or the fan If selected these will be turned on for the duration of the incubation In addition if the preceding dispense excludes pausing then the time to dispense the reagent typica
42. plied X axis Linear Slide Fig 17 Position of X axis Linear Slide 4 Ensure that the tray clamping components in good working order 5 Replace all reagent pump to bottle top tubing the internal pump tubing for all of the Reagent Pumps and the internal pump tubing for the Aspirating Pump Fig 18 Bottles amp Tubing 6 Run instrument through with water and do the following checks a Ensure that the arm moves to the correct position and that the reagents are dis pensed into the centre of the tray wells b Open the lid to ensure that the warning buzzer operates correctly c Ensure that the Green LED is working 7 Check for obvious signs of damage to the instrument 8 Clean the viewing window and casework Bee Robotics July 2009 Page 19 Bee Robotics Ltd B20 User Manual Version 1 5 7 B20 PROGRAMMABLE SOFTWARE 7 1 Introduction The Bee Blot Assay Editor used on the B20 is a rapid editing toolbox to allow the development of custom assays for running on a B20 or a BeeBlot The software consists of two main tools namely the Assay Editor Assay Settings and Instrument Settings The Assay Editor provides an intuitive windowing interface which is wizard based The custom assay is described in the Assay Editor as a number of steps and here reagent preheating and instrument cleaning cycles can be added as required At the stage the developer is satisfied with the custom assay it can be direct
43. r B when selected heater B is turned on throughout this step NOTE IT IS NOT RECOMMENDED TO HAVE THE PRE HEATERS ON FOR LONGER THAN IS NECESSARY Bee Robotics July 2009 Page 22 Bee Robotics Ltd B20 User Manual Version 1 5 The Dispension Phase Controls The dispensing phase for the currently displayed step is specified with these controls this phase contains the majority of the operational settings Firstly consider whether the user requires a pause in operations before dispensing Normally between steps the aspiration and dispense phases are interleaved so the aspiration of one pair of wells will be immediately followed by a dispension to the same wells This is repeated until all wells have been serviced Enabling dispense only will perform distinct aspiration and dispense phases without this interleaving feature This can be enabled for situations that require a time delay to be included between the aspirating phase of the last step and the dispense phase of the current step For example if the previous step warms the tray then for the current step it may be necessary to cool it down with the use of the fan for a short time If the feature is required then select this tick box and number of additional parameters will become visible see Fig 2 Enter the pause interval and whether the tray heater or fan is required to be on during this time This feature is available from the second step onwards The first st
44. s the next assay step Prev Step displays the previous assay step Delete Step deletes the currently displayed step Add Step adds a step after the currently displayed step 7 4 Assay Editing For each step a number of text fields need to be filled tick boxes selected and a pump selected However it is important to understand exactly what is meant by the term step A step in its basic form consists of 3 phases dispension incubation and aspiration The Bee Blot Assay Editor re flects this phased operational cycling and provides a user with an editing window primarily divided into these separate stages see Fig 1 The following texts describe these and at the end of each section a summary of settings is provided for quick reference Step Name The first item to set is the name of the step a text box is provided for entering up to 16 characters This name will appear in the Instruments LCD when the assay is run Step Name Enter a name for this step up to 16 characters long The Overall Step Settings The overall step settings appear in an individual frame below the editing controls These settings apply to the entire step currently displayed Selecting either of these tick boxes will turn on the reagent heaters for the entire duration of this step This would typically be used to maintain the initial preheated temperature of the reagents until they are used Heater A when selected heater A is turned on throughout this step Heate
45. than 20 minutes for the temperature controller to reach the specified temperature than an audible alarm will be heard and the display will indicate heater error The operator must press the Start key on the keypad to silence the alarm The operator will then have the option to continue with the assay without the function of the heater or press the Quit key to abort the as say 4 4 2 Deviation from Set Point Temperature Once the set point temperature has been reached and audible alarm will be activated if the tem perature deviates more than 1 5 degrees from the specified set point temperature The operator must press the Stop key on the keypad to silence the alarm The operator will then have the op tion to continue with the assay without the function of the heater or press the Quit key to abort the assay 4 5 Cleaning Instructions 4 5 1 Cleaning the B20 It is recommended that on a monthly basis that the outside of the instrument is cleaned using anti static foam applied sparingly to the instruments outside panel The operator should switch off the instrument close the lid and apply the foam to one panel at a time A dry lint free cloth should be used to wipe off the foam Particular attention should be made not to spray the foam into the instrument 4 5 2 Cleaning of B20 Trays NOTE DO NOT USE DETERGENTS Residue left from some detergents can affect colour devel opment of strips and increase background
46. the controller has switched off At this point it is necessary to press Start and the operator will then be given the choice of continuing with the assay without the temperature on or to abort the assay Fig 8 Watlow Controller For non heated steps within the assay it is necessary to press the right arrow key on the key pad in order to quit that particular step and move on to the next step within the assay PLEASE NOTE THAT IF THE QUIT KEY IS DEPRESSED DURING NON HEATED STEPS THEN THE ASSAY WILL BE ABORTED f no problems have been encountered the instrument may now be used for analysis Com plete and sign the B20 installation checklist Refer to Appendix 11 and return it to your local representative If you follow this procedure you will be able to observe whether the Pre heaters Fan Watlow Controller and any other parameter selected for the assay are activated at the appropriate times during the assay Preheater A is the furthest out shown on the LEFT here Fig 9 Reagent Preheaters Preheater B is nearest to the pumps shown on the RIGHT here If no problems are encountered during the water run the instrument can now be used to run an asSay Bee Robotics July 2009 Page 11 Bee Robotics Ltd B20 User Manual Version 1 5 4 4 Possible Warning Alarms The B20 has two built in alarm systems with the following features 4 4 1 Attaining Set Point Temperature If it takes more
47. uly 2009 Page 28 Bee Robotics Ltd B20 User Manual Version 1 5 7 6 Instrument Settings A detailed introduction to the Instrument Settings tool will be given in this section This tool allows a user to develop and tune a variety of settings that used within the Assay Editor Starting the Instrument Settings tool To start this tool click on the main menu Options gt Instrument Settings A tabbed window will appear with each tab corresponding to an aspect of the instrument which is detailed below Pump Selector This window provides a convenient means to graphically colour code the reagent pumps see Fig 27 To perform this click on a colour in the colour selector and then click on one of the circles The coloured circles represent the dispensing pumps as follows Top Row from left to right Dispense Pump 1 Dispense Pump 3 Dispense Pump 4 Dispense Pump 6 Bottom Row from left to right Dispense Pump 2 Dispense Pump 5 This colour coding will be used throughout the other tools for the convenience of the user Instrument Settings Cleaning Cycle Setul Cleaning Cycle B Setup Pump Primer Settings m Pump Colour Selector Fig 27 The Pump Selector Window OK Apply This page provides the user with a means to specify individual pump prime times see Fig 28 The user will want to minimise these times whilst preserving adequ
48. y on the instruments keypad and perform a man ual aspirate The operator will also be given the option to carry out the cleaning cycles which have been pre programmed in the instrument settings as described in Chapter 5 section 5 5 We recommend that the Example assay is uploaded into the instrument and a water run is per formed The operator should understand the instrument s performance in accordance with each step of the Example assay This will enable the operator to grasp the software and create his or her own assays It is recommended that the instrument is cleaned at the end of each day and especially at the end of the week or if the instrument is not going to be used for several days Bee Robotics July 2009 Page 31 Bee Robotics Ltd B20 User Manual Version 1 5 7 8 Glossary of Terms Bee Blot Assay Editor Custom Assay Assay Editor Instrument Settings Uploading Cleaning Cycle Slot Preheating the software tools used to develop custom assays and refine in strument settings an user developed assay a convenient software tool for developing custom assays and a component of the Bee Blot Assay Editor software a convenient software tool for refining various system parameters of your B20 the process of transferring a user developed custom assay from the Bee Blot Assay Editor to the B20 instrument the process of washing the reagent delivery system which includes th
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