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1. Repeatability and Accuracy Section 2 ProScan II 7 Prior Scientific Repeatability and Accuracy Accuracy Repeatability and Resolution are important considerations when evaluating stage performance Accuracy is simply defined as the difference between the requested and the actual motion performed by a linear motion device Repeatability is defined as the ability of a device to reproduce a given linear motion Resolution is then defined as the smallest movement or step size the device is capable of Note The resolution of the X and Y axes are usually different from that of the Z axis or focus resolution The Marksman Analogy shown below attempts to pictorially demonstrate the difference between accuracy and repeatability The target on the left shows a cluster of shots that are all in the same basic location yet not in the desired location the centre of the target The marksman was repeatable but not accurate The target on the right has all of the shots close together and at the centre of the target The marksman that took these shots is both accurate and repeatable Some motorized stage manufacturers overstate their stage accuracy by using the Root Mean Square RMS definition of accuracy Prior Scientific uses the Standard Deviation Method When Prior Scientific quotes stage accuracy 3 sigma accuracy 3 Standard Deviations is used This means that 99 74 of all movements made by our stage will be within our stated acc
2. 87 88 Prior Scientific Glossary of Terms Aperture The area which is available for the passage of light Autofocus The ability of a Z focus system to automatically find the correct focus when initiated by either an RS 232 command or the fire button on a joystick Autofocus Score The relative number that represents the contrast for the best image the Autofocus routine has captured Autofocus Range number from zero to five that represents the relative distance the Z focus drive will travel when trying to maximize the Autofocus Score Closed Loop Control A control system whereby the controller adjusts the motor position based on the measurement provided by an electronic linear scale or rotary encoder Coarse Focus Knob The large knob on the side of a microscope that moves the stage up and down large distances with relatively small motion Compatibility Mode The serial communication status which allows the H130 Controller to be backwards compatible with the H127 and H128 controllers See the COMP command Compatibility mode is COMP 1 Controller The device which provides positional control to the stage focus drive filter wheel or shutter Digipot A circular device encoder typically mounted on a joystick used to manually rotate the fine focus knob The digipot rotates the focus knob at an angle relative to the angular movement of the rotation of the digipot Encoder A feedback device which p
3. Replacement Parts Description Specimen Holders for H101 H107 and H117 Stages Glass Plate not suitable for transmitted light microscopy Slide Holder for one 76 x 26mm Microscope Slide Slide Holder recessed for one 76 x 26mm Microscope Slide Slide Holder recessed for one 76 x 26mm Microscope Slide Manual Rotating Holder for one 76 x 26mm Microscope Slide Aluminium Plate Slide Holder for four 76 x 26mm Microscope Slides Slide Holder recessed for four 76 x 26mm Microscope Slides Motorised Rotating Holder 71mm diameter additional axis required Recessed Microtitre Plate Holder 85 x 128mm Petri Dish Holder Universal up to dishes of 100mm diameter Dual Petri Dish Holder for two 35mm diameter Dishes Triple Petri Dish Holder for three 35mm diameter Dishes Recessed Terasaki Plate Holder 55 x 80mm Flask Holder please specify size of flask Chambered Coverslip Holder Holds four Lab Tek Slides with 1 2 4 or 8 chambers Holder for one Mounted Metallurgical Specimen 1 25 diameter Holder for one Mounted Metallurgical Specimen 2 diameter Holder for six Mounted Metallurgical Specimens 1 25 diameter Holder for one Mounted Metallurgical Specimen 1 5 diameter Holder for a single 1 1 25 and 1 5 Mounted Metallurgical Specimen Specimen Holders for H138 Stage Glass Plate not suitable for transmitted light microscopy Slide Holder for eight 76 x 26mm Microscope Slides Aluminium Plate Specimen Holders for H10
4. Restores Index of Stage saz ja __ Sets the current Z acceleration setting a SeeSAZ None Report the current acceleration setting aforZ Sets the current Z acceleration to a Range is 4 to 100 See SAZ O Sets the current x y maximum speed to s Range is to 100 Ls Reportthe current x y maximum speed setting s See SMS Turns ON the joystick This function responds with a 0 in standard SMX 4 Sets the current x y maximum speed to 8 Range is 1 to 0 Report the current filter wheel w acceleration setting 5 5 W w a S ProScan II a les 1 Noe 5 s ba wa Sets the current filter wheel w acceleration to a Range is 4 to 100 Sets the current filter wheel w acceleration to a Range is 4 to 100 See SAF Sets the current Stage x y acceleration to a Range is 4 to 100 See SAS Same as SIS Sets Index of stage 27 58 Prior Scientific reverses direction of stage Reports the Z user units value s 5 5 NE A Sets the value for XY user units to s Entering negative number Sets the value for Z user units to s Entering negative number reverses direction of motor XYBL s b Sets the XY backlash value for joystick to b in pulses 250 pulses per full step of the motor s 1 enables backlash s 0 disables backlash XYBL Reports back s and b for stage see above in form s b XYBLPC sb Sets the XY backlash value for host moves to b in pulses s 1
5. by scanning the image at several different focus positions and returning to the focus position that has the highest score The focus score is based on the contrast of the image using a grey scale integration algorithm Contrast measurements can be made across the whole image over predefined quadrants or the focus area can be user defined see below During the Autofocus routine measurements are made over a range of focus positions and this range can be defined using the A n command where n is an integer in the range 0 5 as shown below There are 50 000 microsteps per revolution of the focus motor Ln Move size in pulses L0 2560microsteps Be El 2 3 20480 microsteps 40960 microsteps Is 81920 microsteps 64 Prior Scientific microsteps 10240 n 1 5120 microsteps 5 When the Autofocus routine is initiated the focus motor is driven down by half of the autofocus range From here the motor is driven upwards through the defined range The motor is driven at a speed which ensures that the focus range is completed after 16 video frames A focus score and its corresponding position is measured for each frame The focus motor is driven to the position which corresponds to the highest focus score From here the above process is repeated but over a range equal to 1 8 of the first range Again the focus score is measured for 16 frames and the motor is driven to the position which produces the highest focus score
6. As a final check the focus score is compared with the focus score at the start point and the motor is driven to the position which produces the highest score When moving to the final position the motor drives the focus mechanism upwards to reduce the effects of backlash in the microscopes focus mechanism The area of the image used for the Autofocus measurement may also be selected The AA n command permits the selection of the whole image or the top left top right bottom left bottom right or central quadrants as the area of interest An additional command AA Lt r b s can be used to define the autofocus area When using this command the image is effectively broken down into a matrix of 11 horizontal by 8 vertical cells PAL or 11 horizontal by 7 vertical cells NTSC The user can select the Autofocus area by entering the position of the top left corner of the area 1 t and the bottom right corner r b The values of r and b must be higher than and t For example if the command AA 2 3 6 5 is used the area selected for autofocus measurements is shown below The optional s parameter may be used to make the screen area visible on the video output ProScan II 65 6 14 AutoFocus Commands Initiates Autofocus procedure N 0t05 0is smallest scan range 1 is twice the scan range etc If no parameter supplied then the assumed rang
7. Scientific When the controller identifies an encoder fitted to any axis for the first power up this prompts a small motion to be initiated two full steps of the motor The controller will use the number and sign of the counts from the encoder to establish the correct ratio between encoder pulses and motor pulses The stage can be sent to a position using internal stepper position or encoders using the ENCODER Command If the stage is fitted with encoders which are disabled the P s and P e Command can be used to compare the stepper and encoder position This facility may be useful for application software where stage calibration is required Stepper moves will be faster than moves under encoder control due to the feedback checking and adjusting required for the closed loop system The SERVO b command gives the option for the controller to constantly read the encoder position when stationary and correct for any position drift b 1 SERVO on b 0 SERVO off This is a global command that affects all axes fitted with encoders With SERVO off the axis still moves to its destination positions measured by the encoder but will not correct for any drift once the destination has been reached This command refers to ALL axes which have encoders fitted and enabled ENCODER none n Reports back as a decimal number the axes that are operating using the encoders For example 3 means X and Y axes are using the encoders s
8. and off if m 0 Setting COMP 1 will result in less flexibility For example SOAK cannot be used and commands sent while the joystick is active will be lost Compatibility mode is offered for users who wish the Commands to be compatible with earlier H127 H128 Prior Controllers Reports Instrument name version number and compile time Note that the system description refers to the presence or absence of internal drivers NOT which peripherals are connected E g H29XY1 can drive XY stage and 1 filter wheel only Sets the reporting of error to Human if h is 1 readable text else error codes are returned see Error Description Table Stops movement in a controlled manner to reduce the risk of losing position In compatibility mode this command is acted on immediately i e there is no need for a CR The command queue is also emptied In Standard Mode a CR must be used Immediately stops movement in all axes Mechanical inertia may result in the system continuing to move for a short period after the command is received In this case the controller position and mechanical position will no longer agree In compatibility mode this command is acted on immediately i e there is no need for a CR The command queue is also emptied In Standard Mode a CR must be used This command is normally treated as an emergency stop Reports the units serial number nnnnn if the serial number has not been set 00000 is returned
9. magnitude n Down at speed set by 3Z 2E n Starts move of relative magnitude n East Right at speed set by 3X Move can be interrupted using the T Command 2N n Starts move of relative magnitude n North Back at speed set by 3Y 2S n Starts move of relative magnitude n South Forward at speed set by 3Y 2U n Starts move of relative magnitude n Up at speed set by 3Z 2W n Starts move of relative magnitude n West Left at speed set by 3X 3X p Sets the X speed as a percentage of maximum for use with 2E and 2W commands 3Y p Sets the Y speed as a percentage of maximum for use with 2N and 2S commands 32 p Sets the Z speed as a percentage of maximum for use with 2U and 2D commands 9 X y Z 0 Sets the Absolute position of x y and z Axis See P x y z 56 Prior Scientific m wr ke apa E NNNM Es None Focus Score lt CR gt only is returned if AutoFocus not implemented is dem R Move to absolute position x y z See G x y z rp I no lt CR gt AutoFocus score value zero is returned 1f Autofocus 1s not implemented Turns OFF the joystick after completion of any current joystick move This function responds with a 0 in Standard mode and a R in Compatibility mode See J 0 R when motors Stops stage gracefully stopped if motors moving No response at all if motors already None stationary None mode and a R in compatibility mode See J 1 RP Same as RIS
10. move for a short period after the command is received In this case the controller position and mechanical position will no longer agree In compatibility mode this command is acted on immediately 1 6 there is no need for a lt CR gt The command Queue is also emptied In Standard Mode a lt CR gt must be used This command is normally treated as an emergency stop Moves stage and focus to zero 0 0 0 Sets the speed of the focus motor under joystick digipot control s is percentage in range 1 to 100 Reports value of OF allowing for joystick speed buttons effect 1f the button speed is 42 and OF is set to 50 the returned value will be 25 Sets absolute position of z axis No axis can be moving for this command to work If an encoder is present on the Z axis the position is only set when the current position is in the encoder range 1t must have been further down than it is currently 48 Prior Scientific RES a r Sets the desired resolution for the axis ais the axis r can be a non integer number setting the resolution for the axis in units of microns UPR command must be implemented before using this command for Z axis e g UPR z 400 RES z 0 1 Resolution set to 0 1 micron for a focus mechanism of 400 microns per revolution of the motor RES la Retums resolution for axis Saz la Jo Sets the current Z acceleration to a Range is 1 to 100 SAZ Nome fa RepotihecurentZaccelerationsetting scz le lo Sets
11. position and the appropriate LED will illuminate Buttons labelled Next and Prev can be used to move the wheel to the next current position 1 and previous current position 1 positions respectively The button labelled Home can be used to re initialise the system by driving it to the home position and then to position 1 5 5 Touch Screen Keypad CSI52KB The Touch Screen Keypad provides a programmable terminal that can be used to control the entire ProScan system Detailed operating instructions are provided in a separate dedicated operating manual Part No W2518 that is supplied with the unit 32 Prior Scientific Advanced Operation Section 6 ProScan II CES 34 Prior Scientific Advanced Operation 6 1 RS232 Command Set ProScan controllers can accept commands from either serial port The port defaults to a baud rate of 9600 This can be increased to 38400 if desired see BAUD below Commands and controller responses are terminated with a Carriage Return code lt CR gt with the exceptions of I K and in compatibility mode Commands are separated from arguments by one or more of the following delimiters COMMA SPACE TAB EQUALS SEMICOLON COLON To move a stage to a position of 100 200 the user could enter any of the following G 100 200 lt CR gt G 100 200 lt CR gt G 100 200 lt CR gt G 100 200 lt CR gt G 100 200 lt CR gt There are two modes of oper
12. power to ProScan II on 5 Select My Computer Control panel System Hardware Device Manager 6 Expand Ports COM amp LPT to see list of ports 7 USB HID gt COM n will now be added Make a note of the COM port number Select this port when writing applications or running a terminal emulation application such as HyperTerminal This will enable communication between the PC and ProScan II via the USB port If HyperTerminal is used none of the default settings within this application will need to be modified Installing USB software on PC running Microsoft XP Professional This does not install automatically Extra care must be taken 1 WARNING Do not connect ProScan II to PC at this point 2 Insert CD Open USB folder and run HidComlnst exe WARNING The only indication that it has installed correctly is the momentary appearance of the hourglass There is no message indicating successful completion 3 Connect ProScan II to PC using USB cable provided 4 Switch ProScan II on 5 Select My Computer Control panel System Hardware Device Manager 6 Expand Ports COM amp LPT to see list of ports 7 If USB HID gt COM n is listed where n is number of COM port then installation is complete If not continue as follows 8 Select Human Interface Device in USB list right click select Properties select update driver select Install from a list or specific location Click Next select Don t search I will choose the drive
13. rotate the motor 1 8 degrees or 1 200 of a revolution The ProScan controller microstep the motors this is a technique whereby the coil current in the motor is precisely controlled to sub divide the fundamental step angle 1 8 degrees of the motor into a series of smaller sub steps called microsteps or pulses The ProScan controller is capable of creating 250 microsteps per full step of the motor Thus for a focus motor attached to a microscope that has a fine focus mechanism with 100 um per revolution of the fine focus shaft the system can achieve the following resolution 200 step rev x 250 micro steps step 50 000 micro steps rev Hence 100 um rev 50 000 micro steps rev 0 002 um micro step Therefore the theoretical resolution of the focus drive motor is 0 002 um pulse For a typical stage with a 2mm pitch screw 2mm per rev or 2000 um per rev the stage has a resolution as shown below 200 step rev x 250 micro steps step 50 000 micro steps rev Hence 2000 um rev 50 000 micro steps rev 0 04 um micro step For a typical stage with a 5mm pitch screw 5mm per rev or 5000 um per rev the stage has a resolution as shown below 200 step rev x 250 micro steps step 50 000 micro steps rev Hence 5000 um rev 50 000 micro steps rev 0 1 um micro step 10 Prior Scientific 2 2 S curve vs Trapezoidal Accelerations Step motors for years have been accelerated with a standard trapezoidal acceleratio
14. s returned a EE NEM Sets the stage backlash value for joystick moves to b in microsteps s 1 enables backlash s 0 disables backlash LI TEILEN enables backlash s 0 disables backlash s returned IF None R Moves Forward by the v step size defined by the X command Moves Forward by y steps Go to the absolute position x y z Z is optional Go Relative Moves by the amount specified by x y z Z is optional IR Move to absolute position x y position remains unchanged Move to absolute position y x position remains unchanged Turns OFF the joystick Stage and Z axes after completion of any current joystick move The joystick is re enabled using J Command see below The joystick is always enabled on power up Turns ON the joystick Stage and Z axes This command is acted upon immediately 42 Prior Scientific LL Sets the direction of X axis under joystick control c 1 Joystick right moves stage mechanically right c 1 Joystick right moves stage mechanically left LI wD Nome a Sets the direction of Y axis under joystick control c 1 Joystick forward moves stage mechanically forward c 1 Joystick forward moves stage mechanically back Reads d 000 Nome R Moves Left by u steps as defined by the X command io k R Moves Left by x steps mo pme R Moves stage and focus to zero 0 0 0 ProScan II Sets the speed of the stage under joystick control s is pe
15. the s curve value for Z in units of in the range 1 10 100 _ m o Seisthecument Z maximum speed to m Range is 1 to 100 uv la JR Movesupbyaseps u Nm R Moves Up by w steps defined by the C command UPR a n Sets the number of microns n linear movement per revolution of the motor for the axis a E g UPR z 100 is set for a motor fitted to the fine focus knob with 100 microns focus movement per revolution urr a In Retumsmicronsperrevolutionforthcaxisa v Ja Go to he absolute position z in the z axis ProScan II 49 VZ s U Sets the focus speed to s in units specified by u u u Units are microns per second u is the default unit and can be omitted To stop a virtual focus move use VZ 0 Note that UPR must be set to achieve correct linear focus speed IZ None Jo Sets the stage and focus absolute position to zero 0 0 0 ZD d 1 Sets direction of rotation of focus motor for commands sent via serial port Defaults to 1 and is correct for motor fitted on right hand side of the microscope d 1 Direction of rotation of focus motor opposite to above 7D fa Remsd 50 Prior Scientific 6 5 Filter Wheel Commands 7 w f R ora w defines the filter wheel number 1 2 or3 f is defined below number If f is a number command moves filter wheel w to filter If no wheel position f is fitted E 17 If f is N command moves filter wheel w to next filter will be If f is
16. 5 Stage Slide Holder for one 76 x 26mm Microscope Slide Glass Plate not suitable for transmitted light microscopy Aluminium Plate Wafer Chuck spring loaded for 75mm wafers Wafer Chuck spring loaded for 150mm wafers Wafer Chuck rotatable with steps for 100mm and 150mm wafers Wafer Chuck rotatable with vacuum for 150mm wafers ProScan II Part Number H220 H224 H224R H224PRV H224ROT H230 H234 H234R H240 H223R H229 H229D35D H229D35T H223RT H23X H236 H656 H657 H658 H659 H649 H288 H238 H239 H226 H227 H231 H143 H146 HWCI5S HWC15V Do Wafer Chuck Pod with vacuum Motorized Rotating Holder 150mm diameter requires four axis controller Motorized Shuttle Insert for automatic wafer loaders 100 amp 150mm wafers Motorized Shuttle Insert for automatic wafer loaders 75mm wafers Specimen Holders for H116 Stage Glass Plate not suitable for transmitted light microscopy Aluminium Plate Wafer Chuck spring loaded for 150mm wafers Wafer Chuck spring loaded for 200mm wafers Wafer Chuck spring loaded for 200mm notched wafers Wafer Chuck rotatable with steps for 150mm amp 200mm wafers Wafer Chuck rotatable with vacuum for 200mm wafers Wafer Chuck Pod with vacuum Specimen Holders for H112 Stage Aluminium Plate Glass Plate not suitable for transmitted light microscopy Wafer Chuck rotatable with steps for 200mm amp 300mm wafers Wafer Chuck rotatable with vacuum for 300mm wafers Wafer Ch
17. Emulation Program Windows 3 1 for communication with a ProScan Controller 1 Double click the ACCESSORIES Group symbol in WINDOWS 3 11 2 Double click the TERMINAL Icon 3 From the SETTINGS menu select COMMUNICATIONS Choose 9600 DaaBis FE Flow Control Connector Coml or Com2 depending on which port is required to be used Parity Check Press OK button 4 From SETTINGS menu select TERMINAL PREFERENCES Choose Columns 8 Of f f IBM to ANSI Buffer Lines Press OK ProScan II 105 5 From SETTINGS menu select TERMINAL EMULATION 6 Choose DECVT 100 ANSD 7 Choose OK 8 Select SAVE AS from FILE menu and save settings as PRIOR 9 These settings can be retrieved at any time by selecting 1t from FILE menu at start of a new session Instructions for using Windows HyperTerminal Emulation Program Windows 95 98 for communication with a ProScan Controller 5 Click Start then Programs then Accessories then HyperTerminal 6 Double click the the Hypertrm icon 7 Enter filename eg priorterm select an icon and press OK 8 For the Connect using option select COM1 or COM2 as appropriate 9 Press OK 10 Enter the following parameters in the Port Setting Box 9600 Databits a 11 Press OK 12 From the menu bar select File and then Properties 13 In the Properties dialogue box select the Settings tab 14 Press ASCII Setup 15 Select Echo typed characters
18. Enter filename e g priorterm select an icon and press OK A window called Location Information will appear press cancel The Confirm Cancel window will appear press yes This will be followed by another window HyperTerminal press OK For the Connect using option select COMI or COM2 as appropriate Press OK Enter the following parameters in the Port Setting Box 9600 ProScan II 107 Duabts 8 Press OK 9 From the menu bar select File and then Properties 10 In the Properties dialogue box select the Settings tab 11 Press ASCII Setup 12 Select Echo typed characters locally and Append line feeds to incoming line ends You will also notice that Wrap lines that exceed terminal width will already be selected 13 Press OK 14 Press OK Instructions for using Windows HyperTerminal Emulation Program Windows XP for communication with a ProScan Controller 1 Click Start then Programs then Accessories then Communications and finally HyperTerminal 2 A window called Default Telnet Program will appear close the box and ignore 3 Enter filename e g priorterm select an icon and press OK 4 For the Connect using option select COMI or COM2 as appropriate 5 Press OK 6 Enter the following parameters in the Port Setting Box 9600 Databits ls Stop bits 7 Press OK 8 From the menu bar select File and then Properties 9 In the Properties d
19. P command moves filter wheel w to previous filter returned If f is F command reports current filter position on filter wheel w If f is H command performs a home routine If f is A wheel will auto home on controller startup If f is D wheel will NOT auto home on startup default 7 3 The first parameter zero indicates all filters the three subsequent parameters fl f2 and f3 are the target positions for filter1 2 and 3 respectively Filters not fitted or invalid filter wheel positions are ignored This feature is only available in COMP 0 mode 7 C Enables automatic shutter closure during any filter move and then re assert initial shutter state at end of move odo lo Disables automatic shutter closure FILTER Text string Prints information about filter wheel w The information end is always a line saying END This allows for the addition of extra fields of information without effecting application software Users should always read lines in until the END is seen in order to maintain compatibility Example FILTER 1 HF110 10 TYPE 3 PULSES PER REV 67200 FILTERS PER WHEEL 10 OFFSET 10080 HOME AT STARTUP TRUE SHUTTERS CLOSED FALSE ee Reports the number of filter positions for filter wheel w ProScan II 51 Report the current filter wheel w acceleration setting Sets the current filter wheel w acceleration to a in units of Range is 1 to 100 ISSE we Sets the cur
20. P 110 Prior Scientific Remove drive cover 4 Loosen limit switch fixing screws do not remove so that the limit switch and clamping plate see fig 2 can be freely moved along the adjustment slot in the limit switch mounting plate Limit Switch Clamping Plate ad gt Fixing Screws Limit Switch Mounting Plate 5 Move limit switch to the desired position and re tighten the fixing screws 6 Before replacing the drive cover mount the stage onto its stand and check that the limits are correctly adjusted If further adjustment is required repeat the above procedure until limit switches are positioned correctly 7 When the limit switches are correctly positioned replace the drive cover ProScan II 111 Specification subject to change without notification Part No W2517 Issue 3 0 11 05 112 Prior Scientific
21. Prior Scientific WAIT ProScan II Reports whether any limit switch is currently active A limit switch is active if the switch is in contact with the axis hardware Nm is a two digit Hex number one Byte which when converted to binary is as follows to binary is as follows D07 D06 DOS D04 D03 D02 DO DOO 4 4 Z 4L Y X 4X eg 05 means stage is in contact with X and Y limit switches OA indicates contact with both X and Y limits 00 means all axes are not in contact with any limit switch Note that the controller knows whether the limit switch is normally low or normally high and corrects accordingly This does not return the hardware signal level of the limit switch see STAGE command AVAILABLE IN STANDARD MODE Reports the units software version number as a 3 figure number oo 100 is Version 1 00 o Inserts a wait of t milliseconds in a macro soak routine 41 6 3 Stage Commands B None R Moves Back by v steps as defined by the X command below Moves Back by y steps Bo jy Pr BLSH s b Sets the stage backlash value for stage move commands sent via the serial port not joystick moves to b s 1 enables backlash s 0 disables backlash B is a number of microsteps of the motor There are 50 000 microsteps per revolution of the motor on a standard ProScan system eee Enables Disables the Stage XY backlash S 1 enables backlash s 0 disables backlash see above In COMP 1 mode only
22. Prior Scientific indicator with sense m When n 0 TTLDEL n Deletes all the trigger points and actions where n TTL input 1 2 3 or 4 associated with TTL input n function disabled default Table of Actions actio Description 68681 data2 3 Filter 1 Move 1 FE 0 0 pos Filter 1 next 2 0 0 Filter 1 previous 3 0 0 Filter 1 Home 4 0 0 Filter 2 Move 5 Pd 0 0 pos Filter 2 next 6 0 0 Filter 2 previous 7 0 0 Filter 2 Home 8 0 0 Move Filter 3 gy ees lo lla pos Filter 3 next 10 0 0 0 Filter 3 previous 11 0 0 0 Filter 3 Home 12 0 0 0 Lumen Light Level 20 0 100 0 0 Move Z Relative 30 Z pos 0 0 Move Z Absolute 31 Z pos 0 0 Move XY Relative 40 Xpos Ypos 0 Move XY Absolute 4 Xpos Ypos 0 Move XYZ Relative 50 Xpos Ypos Zpos Move XYZ Absolute 1 X pos Y pos Zpos Open Shutter 1 60 0 0 0 Close Shutter 1 61 0 0 0 Open Shutter 2 62 0 0 0 Close Shutter 2 63 0 0 0 Open Shutter 3 64 0 0 0 Close Shutter 3 65 0 0 0 Stop All Movement 70 0 0 0 ProScan II La Example 1 Create a Trigger that will be activated when TTL1 goes low and moves the stage and then open shutterl on the next rising edge of that input TTLTP 1 0 TTLACT 1 40 5000 4000 0 TTLTP 1 1 TTLACT 1 60 0 0 0 TTLTRG 1 First falling edge detected will execute the stage command the next rising edge of TTL1 causes the shutter to open Example 2 The openin
23. S Step number of scan Roo Moves to n cell in X and m cell in Y in rectangular snake Sets the origin of the disc grid equal to the current position Zeros the disc grid X and Y counter IR Move to next field of Disc Raster Scan Step number s of circular scan 0 in Sets the diameter in mm of a circular disc Range 1 to 327mm compatibilit The parameter X defining the step sizes in x and y should be y mode defined before setting the W value Number of fields in standard mode E Reports the disc diameter in mm 6 9 HI27 H128 Compatible Commands The following commands are also available There are equivalent commands listed earlier to perform the same function The commands below provide compatibility with previous generations of Prior Controllers H127 H128 Response bit field Reports the current state of the controller Returns bit field giving current status of controller 0 idle 1 busy Format is F2 Fl A Z Y X eg 100000 is filter wheel 2 moving only 000111 is stage and focus moving This command is acted on immediately in compatibility mode there is no need for a lt CR gt Limit switch status see Main Command section above for description Note the addition of Line Feed lt LF gt before lt CR gt 10 None 0 lt LF gt O lt LF gt indicates no AutoFocus fitted only available with ProScan Note the addition of Line Feed lt LF gt before lt CR gt 2D n Starts move of relative
24. SCRIPTION DIMENSIONS WEIGHT L x W x D H101 Upright Stage 112 x 73mm Travel 342 x 249 x 71 3 0Kg H105 Upright Stage 153 x 153mm Travel 400 x 353 x 82 5 0Kg H107 Inverted Stage 112 x 73mm Travel 316 x 314 x 63 3 5Kg H117 Flat Top Inverted Stage H112 Upright Stage 300 x 300mm Travel 548 x 548 x 100 14 5Kg H116 Upright Stage 255 x 215mm Travel 500 x 408 x 82 7 7Kg H138 Upright Stage for 8 3 x1 Slides 426 x 290 x 71 4 0Kg H30 ProScan II Controller 355 x 270 x 100 3 8Kg CS100K Filter Wheel Keypad 150 x 125 x 30 0 35Kg CS152DP 2 Axis Joystick With Remote Focus 200 x 120 x 45 0 5Kg CS152EF 2 Axis Joystick With Remote Focus 200 x 120 x 45 0 5Kg and Autofocus Button CS152KB Touch Screen Keypad 230 x 180 x 75 1 6Kg CS152V2 2 Axis Joystick 200 x 120 x 45 0 35Kg CS152V3 3 Axis Joystick 200 x 120 x 45 0 45Kg CS152Z Digipot Z Axis Only 130 x 100 x 55 0 7Kg HF200 Shutter 25mm 100 x 85 x 25 0 3Kg HF201 Shutter 32mm 112 x 92 x 28 0 5kg HF108 8 Position Filter Wheel 32mm 160 x 178 x 27 1 4kg HF110 10 Position Filter Wheel 25mm 140 x 168 x 27 1 2kg Max Speeds Stage Focus Drive Filter Wheel Shutter Power Universal integral power supply Input 90 265V 110 240V 50 60Hz 60W Output 24VDC 2 5A Specifications subject to change without notice ProScan II Up to 100mm sec 20 revs sec 55 msec Position to Position 14 msec for open close cycle 85 86 Prior Scientific Glossary of Terms Section 9 ProScan II
25. Table of Contents Important Safety Information Section 1 Repeatability and Accuracy Section 2 2 1 Step Motors and Resolution 2 2 S curve vs Trapezoidal Accelerations Unpacking The System Section 3 3 1 Other Accessories Installation Section 4 4 1 Removing an Existing Stage 4 2 Fitting the ProScan Stage 4 3 Cable Connections 44 USB Operation 4 5 Focus Drive Installation 4 6 Installing Filters 4 7 Mounting Filter Wheels and Shutters Getting Started Section 5 5 1 Using Joysticks 5 2 Standard Features 5 3 Using the Z Axis Digipot CS152Z 5 4 Using the Filter Wheel Keypad CS100K 5 5 Touch Screen Keypad CS152KB Advanced Operation Section 6 6 1 RS232 Command Set 6 2 General Commands 6 3 Stage Commands 6 4 Z axis Commands 6 5 Filter Wheel Commands 6 6 Shutter Commands 6 7 Lumen Pro Commands 6 8 Pattern Commands ProScan II 10 11 t3 16 17 19 19 20 20 22 24 25 27 29 30 31 31 32 33 35 38 42 47 51 53 54 55 6 9 H127 H128 Compatible Commands 56 6 10 Error Codes 60 6 11 CS152 Joystick Configuration 61 6 12 Examples 63 6 13 Connecting the Video Autofocus 64 6 14 AutoFocus Commands 66 6 15 Encoders 68 6 16 TTL input output signals 72 6 17 TTL Command set 13 6 18 TTL Programming Advanced Features 14 Troubleshooting Section 7 77 System Specifications Section 8 83 Glossary of Terms Section 9 87 Replacement Parts Section 10 93 Returns and Repairs Section 11 99 Appen
26. a fields of information without effecting application software Users should always read lines in until the END is seen Example STAGE H101 2 TYPE 1 SIZE_X 108 MM SIZE Y 71 MM MICROSTEPS MICRON 25 LIMITS NORMALLY CLOSED END None Returns the skew angle a in degrees that had previously been set by the SKEW A and SKEW S commands or the SKEW A command ProScan II 45 Skew About command when used in conjunction with SWEW S can re align samples which are not perfectly aligned to the XY motion of the stage Use this command to skew the XY stage movement About this point The SKEW S command must also be executed to complete this operation Use this command after the SKEW A command AND moving a distance in X and Y axis to define the skew angle This 1s equivalent to twisting the stage Sets the skew angle a IE SKEW 0 5 will skew the stage through degrees SKEW 0 disables skew function ma Nw o Toggles Y axis of joystick between Y and A axis control X y u Sets the stage speed to x y for the X and Y axes respectively in units specified by u u values in microns linear travel per second This is default if u is omitted therefore VS 500 500 would set 500um s in both X amp Y u p are values in microsteps per second based on 250 microsteps per full step of motor VS 500 500 p would set 2 full steps per second To stop this virtual joystick move use VS 0 0 If limits are hit the speed will be set to zero for
27. ation Standard Mode and Compatibility Mode Standard mode is the recommended mode for new software as it offers more features Compatibility mode is supported for existing customers who do not wish to re port their existing application code All communication is non blocking meaning that commands can always be sent even though there may be a delay prior to their execution In Standard Mode up to 100 commands may be queued in the serial buffer of the controller If a command is sent and there is insufficient space to accept it an error E18 Queue Full will be returned This indicates that the command has not been accepted and must be resent when the queue is no longer full It is desirable to read back each command R lt cr gt before sending any further commands Sending I lt cr gt aborts the current move and empties the queue ProScan II 5 The default convention is that the controller will move each device by lum per number entered in other words a requested move of 1000 0 will result in the stage moving 1mm in the X axis If desired this can be over ridden by using the scale stage SS command If the stage scale is changed the resultant movements are determined by the model of stage in use The controller is fixed at 250 micro steps per full motor revolution and by setting SS 1 each requested move will be in micro steps A requested move of 1000 0 will now result in the stage moving by 4 1000 250 complete motor revolutions The a
28. closed loop servo control e Superior precision and finer resolution Linear scales mounted on Prior stages now provide repeatability to 0 3 microns and resolution step size as small as 0 1 microns This superior performance is extremely critical in many Industrial and Bio Science applications such as the performance of time lapse photography studies H130 Controller with the E option enables rotary or linear encoder with RS485 differential quadrature square wave outputs to be inputted to all or any of the 4 axes This controller will have 3 or 4 round 12 pin connectors on the back of the control box labelled X Axis Y Axis etc Turn the controller off and plug in the appropriate encoder or scale that corresponds with the appropriate axis The linear scales also have a home or reference position This is a specific mark on the scale that is read by the controller and it can be used to establish the same reference position at any time To use this feature see the commands SIS and RIS described below When an encoder is fitted and enabled the default condition the controller uses the position measured by the encoder as the true position and not the number of pulses sent to the stepper motor The controller will sense which encoder input has an encoder fitted It will control the individual axis using motor pulses or encoder pulses depending on whether that axis has an encoder fitted and enabled 68 Prior
29. ctual distance moved by the stage will depend upon the pitch of the ball screw fitted to the stage A stage with a 2mm ball screw pitch will move 8mm in the X axis while a unit with 5mm pitch screws would move 20mm The commands STAGE FILTER FOCUS and SHUTTER return a text description the last line of which is always END The allows Prior to add supplementary text information without resulting in changes to the users application code assuming that the application software reads al text up to END Macro and Soak MACRO a set of commands can be entered and started in a block by the use of the MACRO command Example of MACRO If you wish to close a shutter move the filter wheel to a new position then open the shutter MACRO 0 enter macro mode 8 A 1 R close shutter A 7 1 4 R move to filter position 4 8 A 0 R open shutter A WAIT 1000 R wait 1000 msecs MACRO 0 start the macro SOAK this is an extension to the MACRO command enabling the testing of a controller without tying up a PC The soak routine continually performs the instructions entered in a loop reporting the number of complete cycles on each pass To stop the soak test enter an action and the unit will complete the current cycle and then stop 36 Prior Scientific Example of SOAK If you wish to test a shutter and filter wheel you could use the following routine SOAK 0 8 A 1 R 7 1 4 R WAIT 500 8 A 0 R 7 1 1 R SOAK 0 enter soak mode close
30. dices Section 12 LOS 12 1 How to Run HyperTerminal 105 12 2 Adjusting Stage Limit Switches 110 2 Prior Scientific Important Safety Information Section 1 ProScan 3 Prior Scientific Important Safety Information Save this manual as it contains important safety information and operating instructions Before using the stage system please follow and adhere to all warnings safety and operating instructions located on the product and in this User Manual Do not expose the product to water or moisture Do not expose the product to extreme hot or cold temperatures Do not expose the product to open flames Do not allow objects to fall on or liquids to spill on the product Connect the AC power cord only to designated power sources as marked on the product Make sure the electrical cord is located so that it will not be subject to damage To reduce the risk of damage unplug the product from the power source before connecting the components together DANGER never alter the AC cord or plug If the plug will not fit into the outlet have a proper outlet installed by a qualified electrician Use only the proper type of power supply cord set provided with the system for this unit Do not attempt to disassemble the product Doing so will void the warranty This product does not contain consumer serviceable components Service should be performed by Authorised Service Centres ProScan II gt Prior Scientific
31. e Autofocus has caught This will be the maximum number caught during the Autofocus procedure and should represent the image with the best visual focus This in turn is the image showing after the Autofocus procedure If the Autofocus Score does not reach a maximum value during Autofocus the focus is returned to the starting position Sets Intensity of the greyed out area of the image see AB above n is in range 0 to 255 As n increases from 0 to 255 inactive area becomes more intense and the active window less intense Sets the default range n for Autofocus initiated using the fire button on the end of the joystick Reports the Focus Score m for the current image ProScan II 67 6 15 Encoders Prior stages can be equipped with either linear or rotary encoders for higher positioning repeatability and accuracy A linear encoder is an optical system that reflects light off a graduated scale through a grating and onto photo sensors These sensors generate electrical currents The electrical currents generated are used to determine distance and direction the stage has travelled Unlike rotary encoders which imply a distance travelled by measuring the rotation of the motor linear encoders scales are actually mounted to the moving plates of a stage and therefore directly measure stage movement Linear Encoders benefit the stage user by providing e The ability to use a Digital Read Out DRO e The ability to provide
32. e for upright microscopes Model H107 H117 Stage for Inverted Microscopes Model HF110 25mm 10 position Filter Wheel for Microscopes includes 15 pin cable or Model HF108 32mm 8 position Filter Wheel for Microscopes includes 15 pin cable High speed shutter Model HF202 25mm aperture or model HF204 32mm aperture Model H30 Controller ProScan II Other accessories may be included with your ProScan system These are not included in the basic ProScan kits and should be listed separately on your order and the packing list You will find a list of the most frequently supplied accessories below 3 1 Other Accessories Model Number CS100K CS152KB H122KLC H122KON H276K H277 HF200 HF202 HF201 HF204 HF205 HF206 HF207 HF210 HF215 HF220 HF230 HF235 HF240 HF245 HF535 HF559 HF568 Description Keypad for Manual Control of 2 Filter Wheels and 3 Shutters Touch Screen Keypad Solid Couple Adapter for Focus Drive on Leica Solid Couple Adapter for Focus Drive on Olympus Nikon RS232 cable for PC 9 or 25 pin RS422 cable for Macintosh 8 pin Mini Din to 9 way D Type High Speed Shutter 25mm aperture for use when mounting to filter wheel High Speed Shutter 25mm aperture for stand alone use High Speed Shutter 32mm aperture for use when mounting to filter wheel High Speed Shutter 32mm aperture for stand alone use Dual Slide in Filter Holder for 25mm Filter Wheels Dual Slide in Filter Holder for 32mm Filte
33. e is that set by the AJ command n Specifies the area of the screen over which the Autofocus is active The visibility of the active area remains the same while the rest of the image which will not be sampled by the Autofocus is either blanked out entirely or its visibility reduced See AB Command n 0 Whole screen active Default n 1 Top left corner n 2 Top right n 3 Bottom left n 4 Bottom right n 5 Centre This allows the user to define an alternative active area The screen is divided into 11 x 8 cells PAL or 11 x 7 cells NTSC and this command allows the definition of a specific area utilising adjacent blocks 1 100 11 Position of left edge t 1to8 Position of top edge r 1to11 Position of right edge b 1to8 Position of bottom edge n Greys out the area of the image where the Autofocus is inactive This is useful for defining the required active area see AA below N 0 Restores the complete image to normal intensity Active area of Autofocus is still as defined using AA below 66 Prior Scientific EN rm Initiates a user defined Autofocus procedure d delay in milliseconds between each image capture n number of image captures in the scan s step size between image captures User Units m divisor for the second scan e g n 4 results in second scan being size of first scan Auto Focus Score Reports a positive integer n which represents the contrast for the image th
34. e keypad will be activated indicated by a red LED Three buttons are provided to control up to 3 shutters Fourteen buttons are provided to control up to 2 filter wheels The button labelled P is for future developments and currently has no function ProScan II 31 The ProScan system can control up to 3 shutters When the system is powered up all shutters connected to the controller will be closed Buttons S1 S2 and S3 can be used to open and close shutters S1 S2 and S3 respectively An illuminated red LED on the button indicates that the shutter is open For example when the system is switched on shutter S1 will be closed and the LED on the button S1 will be off Pressing the button S1 will open the shutter and the LED will illuminate Pressing button S1 again will close the shutter and the LED will go out The ProScan system can control up to 2 filter wheels As previously mentioned when switching the system on the filter wheel will automatically initialise itself by moving to the home position a sensor which the system uses to ensure accurate alignment and then moving to position 1 The button labelled Wheel is used to select the active filter wheel and this is indicated by a green LED A red LED will also illuminate on one of the buttons labelled 1 10 to indicate the current position of the wheel To change the position of the filter wheel press the appropriate button 1 10 The filter wheel will move to the new
35. ecting the joystick diagonally will cause the stage to vector in 2 axes providing a corresponding diagonal movement The joystick provides proportional control The further the joystick is deflected from the central position the faster the stage will move Deflecting the unit to its extreme limit provides the fastest stage movement The CS152V3 joystick is equipped with a twist action proportional control knob for focus control on top of the joystick shaft The Hot Key to the left of the joystick can be used to quickly adjust the maximum speed of the stage This affects both X and Y axes equally The key to the right of the joystick provides an identical function for the focus motor 3 axis joystick only Pressing these buttons once reduces the speed to 25 of maximum Pressing a second time increases the speed to 50 of maximum and a third press of the button returns to 100 of maximum speed This cycle can be repeated by continuing to press the buttons The action of the Hot Keys can be reprogrammed using RS232 commands See Section 6 CS152DP and CS152EF In addition to the standard features described earlier the CS152DP and the CS152EF provide a digipot control mounted to the side of the joystick unit This device controls the focus motor on a 3 axis system It is not proportional but is designed to closely match the normal response of the fine focus knob on a microscope The CS152EF is also equipped with an a
36. ed NEN Sets the output from the shutter to n where n can be set between 1 100 The command automatically locates LGG_SHUTTER If n is h the shutter will perform a home routine VD oo QN tA A o N LIGHT Sets the filter wheel position P to n light output 1 e LIGHT 4 45 Sets position 4 to 45 light output Use 7 n 4 to move filter to position 4 where n is the Filter Wheel port which the shutter is attached STANDARD 10 Position Shutter settings are Position Light output 1 0 1 22 33 44 55 66 77 88 LIGHT CE C Reports n the output of position p 54 Prior Scientific 6 8 Pattern Commands Arguments Command E z UN 34 lt lt gt ar n None n m None None n m XV None None n m None None ProScan II Sets the origin for the pattern to the current position Zeros the pattern X and Y counter If b 0 the stage returns to the origin after completing the final move of the pattern default setting upon power up If b 1 the stage will stay at the final pattern position Ht Defines the number n of X and number m of Y steps for the pattern Reports number of X and Y steps RO Move to next position in Rectangular Raster S Step number of scan Moves to n cell in X and m cell in Y in rectangular raster loo Sets the step size in X and Y x y Reports step size in X and Y Ro Jj Move to next position of Rectangular Snake
37. els and Shutters Prior Filter Wheels and Shutters are supplied with the correct adapter flanges fitted for the specified microscope stand Therefore the mounting of this equipment uses exactly the same procedure used when fitting the microscopes lamphouse All Prior Filter Wheels and Shutters are manufactured with C mount threads The addition of a male to male C mount adapter Part No HF207 allows these units to be mounted to a microscope camera port 1f required Confirm that the ProScan controller unit is switched off before connecting filter wheels to the controller with leads provided see fig 4 3 ProScan II 25 26 Prior Scientific Getting Started Section 5 ProScan II 2 28 Prior Scientific Getting Started Switch the ProScan controller unit on using the on off rocker switch located on the front panel There are three LED s on the bottom left of the front panel The running LED should be illuminated to indicate correct operation If this is not the case refer to section 7 The TX transmit LED will flash rapidly when data is being transmitted by the controller and RX receive LED flashes rapidly when receiving data from computer RS232 The ProScan system can be computer controlled via the RS232 serial port or stand alone using the optional accessories available The ProScan controller provides a plug and play facility meaning that all correctly connected peripheral devices will be automatically confi
38. enables backlash s 0 disables backlash See BLSH ie BLSH e 1 Sets the Z backlash value for joystick digipot to b in pulses s 1 enables backlash s 0 disables backlash ZBL None __ Reports back s and b for Z axis see above in formsb os i Sets the Z backlash value for host moves to b in pulses s 1 enables backlash s 0 disables backlash See BLZH Reports back the Z backlash value for host moves in form s b See BLZH ProScan II 59 6 10 Error Codes If a command is not valid a response of E n is returned The n specifies an error type as listed below Machine or human readable messages are chosen using the ERROR Command a O o O 2 NOTES o INVALIDSHUTTER lg VALUEQUTOFRANGE 3 3 3 9 INVALIDWHEED 0000000008 ARGIOUTOFRANGE mo ARG2OUTOFRANGE O o j ARG3OUTOFRANGE 13 ARG4OUTOFRANGE O Z o 4 ARGSOUTOFRANGE o lis ARG6 OUT OF RANGE 6 INCORRECTSTATE O Z o WHEELNOTFITTED i QUBUEFUL 0000000008 19 COMPATIBILITY MODESET 20 SHUTTERNOTEMTED J INVALIDCHECKSUM AAA C59 60 ENCODERERROR o ENCODERRUNOFF 3 3 3 o 1 2 10 11 12 13 14 15 16 17 18 19 20 21 61 60 Prior Scientific 6 11 CS152 Joystick Configuration The CS152 range of joysticks is compatible with the ProScan system and any one can be used depending on the system configu
39. ets the level of TTL_OUT m 0 is TTL low m 1 is TTL high Note that it is important not to omit m or it will be assumed by the controller that n is a Hexadecimal number ProScan II 73 n Returns m the TTL status of bit n where n is the data bit number of the m 0 is TTL low TTL m 1 is TTL high TTL_OUT has n between 0 and 3 TTL_IN has n between 8 and 11 for backwards compatability with H129 See Hexadecimal nomenclature above 6 18 TTL Programming Advanced Features More complex features are available that provide the user with the ability to construct lists of actions to be executed when TTL input lines change state There is one list per TTL input Each list contains one or more trigger points and each trigger point has associated with it one or more actions A trigger can be activated by a rising or falling edge of the TTL input TTLTP nm Adds a trigger point to the TTL input n aM where n TTL input 1 2 3 or 4 where m 0 falling edge or low m rising edge or high n action datal data2 data3 activated by a change in the level m Add an action to the current trigger point for the TTL input n See table following for full list of actions Resets the Trigger list for input n back to the start Arms the global trigger flag Selects a TTL output n as a motion where n TTL input 1 2 3 or 4 where m 0 Disarmed 1 Armed TTLMOT n m where n TTL input 1 2 3 or 4 where m 0 low 1 high 74
40. f 3 below Z motor constant velocity up Z motor constant velocity down Toggles digipot speed 100 50 25 Toggles X Y joystick speed 100 50 25 Toggles joystick speed 100 10 Toggles joystick speed 100 2 Moves Z motor up by amount defined by C Command Moves Z motor down by amount defined by C Command Moves stage left defined by X Command Moves stage right defined by X Command Moves stage back defined by X Command Moves stage front by amount defined by X Command Toggles Digipot Speed 100 10 Toggles Digipot Speed 100 2 f 16 Starts Auto Focus f 23 Next Point f 24 Next Wafer f 25 Smooth Stop T f 26 Sudden Stop K f 28 Toggle Shutter 1 f 29 Toggle Shutter 2 64 Prior Scientific f 30 Toggle Shutter 3 f 35 assigns any following text to the button Hence any command may be assignable to a button 6 12 Examples Right button b 1 being pressed moves Z up at constant velocity Left button b 2 being pressed moves Z down at constant velocity Release buttons b 0 stops Z motor no buttons being pressed BUTTON 1 2 BUTTON 2 3 BUTTON 0 1 BUTTON 1 35 TYA assigns the TYA command to button 1 BUTTON 1 35 G 100 200 300 executes a G 100 200 300 command Right button b 1 toggles joystick speed 100 2 A momentary press of Left button b 2 starts Z motor moving down with constant velocity Pressing Fire button b 3 stops Z motor BUTTON 1 7 BUTTON 2 3 BUTTON 3 1 Normal Joystic
41. g of the shutter can be automated by wiring the output of the TTLMOT to another TTL input TTLTP 1 0 TTLACT 1 40 5000 4000 0 TTLTP 2 1 TTLACT 1 60 0 0 0 TTLMOT 2 1 TTLTRG 1 76 Prior Scientific Troubleshooting Section 7 ProScan II 77 78 Prior Scientific Troubleshooting Problem Stage will not respond to Joystick or RS232 commands Suggestions Check that the system is properly installed and that there is proper power to the controller For Installation see section 4 Check that the controller is switched on Check that the green Running light is on If it is not return unit to your local Prior Scientific dealer for servicing There is no external fuse that can be replaced Set up and run HyperTerminal Windows Users see Appendix C and send the command via RS232 1 The controller should report with information about the peripherals currently connected as shown in section 6 of this manual If the controller does not acknowledge the presence of any of the connected peripherals return that peripheral to your local Prior dealer Problem In one axis X or Y the stage will only move in one direction Suggestions There is an internal switch failure Return the stage to your local Prior Dealer for servicing Problem The stage will not respond to my Image Analysis Software Suggestions Check that the Image Analysis Software Program has drivers for Prior systems If not consult the sof
42. gured for use when the system is powered up Older ProScan stages are not plug and play compatible If you are upgrading an existing stage system with a new ProScan controller you may need to have the stage upgraded to ensure plug and play compatibility Contact your local Prior dealer for further advice Control via RS232 will be considered further in Section 6 Control via other system accessories including joysticks Z axis digipot filter wheel keypad and touch screen keypad will be described here 5 1 Using Joysticks Four joysticks are available as part of the ProScan system a two axis joystick CS152V2 and three axis joysticks CS152V3 CS152DP CS152EF The joystick units are used to control the motorized stage and the focus motor Only three axis systems will control both the stage and the focus motor gt 7 CS152V2 ProScan II 29 F gt CS152DP CS 52EF 5 2 Standard Features All the joysticks feature an X Y joystick two sliding tensioners and two Hot Keys Ensure the sliding tensioners are fully home and latched to hold the joystick vertically in the off position In this position there is no power to the stage motors and the stage does not move Deflecting the joystick left or right from the central position will cause the stage to move in the X axis Deflecting the joystick backwards or forwards from the central position will cause the stage to move in the Y axis Defl
43. hat the controller is switched on Check that the filter wheel cable is properly connected If using the Filter Wheel Keypad check that the filter wheel cable is properly connected and that the LED s on the keypad flash momentarily when turning on the controller This will confirm power is being provided to the keypad If the LED s do not flash contact your local Prior representative If using the RS232 commands ensure that the filter wheel cable is connected to the correct connector on the back of the ProScan controller Ensure the correct commands are being used for the appropriate filter wheel 1 or 2 ProScan II 81 Problem The filter wheel is noisy when rotating Suggestions Tighten all the filter locking rings with the tool provided Problem The Shutter will not operate Suggestions Make sure that the controller is switched on Check that the shutter cable is properly connected If using the Filter Wheel Keypad check that the cable for the keypad is properly connected If using the RS232 commands determine which connector the shutter cable is plugged into There are 3 shutter connections in the back of the ProScan Controller labelled Shutter S1 S2 and S3 Ensure you are sending the correct commands for the connection being used 82 Prior Scientific System Specifications Section 8 ProScan II 83 84 Prior Scientific System Specifications All Dimensions in mm PART No DE
44. he controller After binary conversion convention is as follows F Fl A Z Y X DOS D04 D03 D02 DOI 0 Optional parameters a where a is the axis or resource X X axis Y Y axis S X and Y axis Z Z axis A A axis not present on Optiscan F Filter wheels Fl Filter wheel 1 F2 Filter wheel 2 when the optional parameter is used the binary word is just for the axis requested Stage is for x y axis F is for filters and would return 0 to 3 depending on if they are in use Sets the baud rate of the port issuing the command to the value specified by b As a protection measure if no command is sent to the port while the controller is switched on the baud rate will revert to 9600 after switching off and back on again twice Allowable values for baud rate are 9600 argument 96 19200 argument 19 and 38400 argument 38 WARNING If the baud rate of ProScan is changed it is important for the application software to check communication with ProScan by scanning the baud rate on initialisation This will avoid a permanent communication failure should the PC Port and ProScan port be set at different bauds Report the Command protocol Compatibility mode 1 or Standard mode 0 er E EHE MACRO None Used to enter and leave the Macro Mode ONLY AVAILABLE IN STANDARD MODE 40 Sets the controller compatibility mode for users who want to wait for R at the end of the move Compatibility is on if m 1
45. hunting when servo enabled Argument e indicates encoder position If ENCODER 0 returns encoder position at present stepper position Use P Command If ENCODER Returns encoder position This is the same as P Command Set Index of stage The stage will find the encoder Index signal stop and set absolute position to 0 0 Used only when the mechanical position of the stage bears no relationship to controller position i e when the stage has been mechanically moved during power off THIS COMMAND MUST BE DONE ONCE AT INITIAL CONNECTION OF STAGE TO CONTROLLER IN ORDER TO ESTABLISH A UNIQUE REFERENCE POSITION WHICH IS PERMANENTLY REMEMBERED BY THE CONTROLLER 70 Prior Scientific RIS none Restore Index of Stage Used to restore accurate mechanical position by seeking Index signal and returning back to controller position This is used to re establish mechanical accuracy by referencing back to the encoder index signal ProScan II 71 6 16 TTL input output signals The H130 controller has the capability of reading and sending signals to and from other external equipment through its standard TTL port This powerful capability allows the controller to process data and make decisions based on other external sources such as pressure switches line scan cameras and relays The ProScan controller has a 10 way boxed header male Four pins are assigned to TTL output one Byte from the Controller and Four pins to TTL in
46. ialogue box select the Settings tab 10 Press ASCII Setup 108 Prior Scientific 11 Select Echo typed characters locally and Append line feeds to incoming line ends You will also notice that Wrap lines that exceed terminal width will already be selected 12 Press OK 13 Press OK ProScan II 109 Appendix B 12 2 Adjusting Stage Limit Switches Introduction Every ProScan motorized stage is fitted with limit switches in both the X and Y axes so that the stage automatically comes to a halt when it has reached its maximum travel in all directions These limit switches are normally factory set to give the correct amount of movement for a particular microscope stand It may be necessary at some time to make adjustments to the limit switch positions For example different optics may need to be mounted onto the microscope which may cause clearance problems to avoid damage to these components the stage travel may need to be restricted The following procedure explains how to make these adjustments Tools Required Small flat blade instrument screw driver Adjustment Procedure Due to the positions of the drive cover fixing screws 1t may be necessary to remove the stage from its microscope or mounting stand before attempting this procedure 1 Ensure that the stage is disconnected from the controller before removing any covers 2 Loosen and remove the drive cover fixing screws see fig 1 Figure
47. ing hold of it and turning it If the adapter is correctly fitted 1t will stay attached to the coarse knob 7 Slide the focus motor into the adapter as far as it will go and while applying gentle pressure to the motor tighten the clamp screw This will hold the motor in place The rubber drive bush on the end of the motor spindle should now be pressing against the end surface of the fine focus control knob This can be confirmed by manually rotating the exposed fine focus knob on the opposite side of the microscope and feeling for the resistance caused by the detent positions of the stepper motor as it rotates This will not cause any damage to the focus motor 8 Confirm that the controller is switched off before connecting the 9 way D type plug on the focus motor lead to the socket on the rear of the controller as shown in fig 4 3 ProScan II 23 4 6 Installing Filters It is recommended that filters be installed before mounting the filter wheel to a microscope 1 Select filter position required for loading by checking the number displayed in the load position indicator window A see fig 4 5 2 Remove the magnetic covers B from the load port 3 Remove lock ring C by unscrewing from the filter holder cell with the tool D provided 4 Insert desired filter and replace lock ring 5 Repeat this process for all the desired filter positions 6 Replace magnetic covers 24 Prior Scientific 4 7 Mounting Filter Whe
48. k operation 6 Left button b 2 toggles Stage speed 100 50 25 Right button b 1 toggles Z motor speed 100 50 25 BUTTON 3 0 BUTTON 2 0 BUTTON 1 0 BUTTON 0 0 BUTTON 2 5 BUTTON 1 4 BUTTON 0 0 ProScan II 63 6 13 Connecting the Video Autofocus Connect the video output of the camera to the H130 controller box via a coaxial cable A BNC connector is required at the controller end of the cable Plug the cable into the connector labelled Video In A second BNC cable is used to connect the monitor to the connector labelled Video Out Video Input The Auto focus board currently accepts composite video in standard PAL 25 frames per second interlaced or NTSC 30 frames per second interlaced formats Both formats can be colour or monochrome The video input is via a BNC and is terminated on the auto focus board with a 75 Ohm load t should not be connected by a T piece as was done on the H128 Note that the H130 video autofocus is not equipped for video autofocus when using a digital camera with only a digital signal output An analogue or video composite signal output is required Video Output This is a 75 Ohm BNC output which will normally be a buffered version of the input signal except for low intensity video areas of the screen see AA command below The signal will only be present when the controller is turned on The Autofocus Method The Autofocus system operates by determining a focus score
49. locally and Append line feeds to incoming line ends Then click OK twice Instructions for using Windows HyperTerminal Emulation Program Windows NT for communication with a ProScan Controller 1 Click Start then Programs then Accessories then HyperTerminal 2 Select the HyperTerminal icon 106 Prior Scientific 3 In the Connection description dialog box enter filename e g prior select an icon and press OK 4 In the Connect to dialog box enter Phone Number if required and Select COM1 or COM2 as appropriate and press OK 5 Enter the following parameters in the Port Settings box Bits per second 9600 Data bits 6 Press OK button 7 Select File Properties and Settings tab 8 Press ASCII Setup 9 Select Echo typed characters locally and Append line feeds to incoming line ends you should find that Wrap lines that exceed terminal width has already been selected 10 Press OK 11 Press OK Instructions for using Windows HyperTerminal Emulation Program Windows 2000 for communication with a ProScan Controller 1 2 3 4 3 6 7 wn Click Start then Programs then Accessories then Communications and finally HyperTerminal A window called Location Information will appear press cancel The Confirm Cancel window will appear press yes This will be followed by another window HyperTerminal press OK
50. n An example of a trapezoidal acceleration is shown below Speed Time This method of motor acceleration has a great impact on the performance of the system The trapezoidal acceleration causes the stage to jerk as it starts switches from acceleration mode to maximum velocity mode and switches from maximum velocity mode to deceleration mode Note all of the sharp corners on the graph This can cause the stage to vibrate and it can increase stage settling time The ProScan controller accelerates the stage via the S curve Speed as shown below The S curve acceleration transitions from starts to stops with smooth accelerations and decelerations thereby resulting in faster quiet and vibration free performance The stage can actually be moved faster due to the ramp characteristics of the S curve Settling time is greatly reduced and overall stage performance is enhanced ProScan II EMI NA Prior Scientific Unpacking The System Section 3 ProScan II 13 14 Prior Scientific Unpacking The System Each ProScan System will consist of the ProScan Controller with its specific model number and one or more of the following components Component A aw ps Component Description Model CS152Z Digipot Z only Model CS152EF Joystick 3 axis other joysticks are available Model H122 Focus Drive Model H122AXIO Focus Drive Model H101 Stage for Upright Microscopes other designs of stage are availabl
51. nly s returned None w Reports the current step size for the z axis focus motor Reports the current step size for the z axis focus motor Ee Sets the current step size for the z axis focus motor to w D tz Movesdowm byasteps Do Nome R Moves down w steps defined by the C command FOCUS None Text string Prints information about z axis focus unit There are 250 microsteps per full step The information end is always a line saying END This allows for the addition of extra fields of information without affecting application software Users should always read lines in until the END is seen to keep in sync Example FOCUS NORMAL TYPE 0 MICRONS REV 100 END ProScan II 47 H None Turns OFF the joystick Stage and Z axes after completion of any current joystick move Joystick is re enabled using J Command below The joystick is always enabled on power up I None Stops movement in a controlled manner to reduce the risk of losing position In compatibility mode this command is acted on immediately i e there is no need for a lt CR gt The command queue is also emptied In Standard Mode a lt CR gt must be used J None Turns ON the joystick Stage and Z axes This command is acted upon immediately Sets the direction of Z axis under digipot control 6 1 None d Readsd Immediately stops movement in all axes Mechanical inertia may result in the system continuing to
52. ntroller with the cable provided see fig 4 3 ProScan II 19 4 3 Cable Connections The cable connections to the ProScan controller are located on the rear panel of the control box as shown in the illustration below Before making any of these connections ensure that the ProScan controller is switched off Each connection is well labelled but great care should be taken not to try and connect your computer s serial port cable to the Z axis connector on the controller The RS232 connection from your computer should be made to the RS232 1 port on the controller For USB connection to your computer see separate section on this subject Fuse Video Shutters in out Oysti Digipot Filter Wheels Encoders Es a 9 6 pcs RE K RS232 Touchscreen TILVO USB s Or keypad 4 4 USB Operation To use the ProScan II controller H130 series via the USB connection the user will need to install the USB connection software This can be found in the USB folder on the CD Installing USB software on a PC running Microsoft Window98 2000 1 WARNING Do not connect ProScan II to PC at this point 2 Insert CD open USB folder and run HidComInst exe WARNING The only indication that the utility has installed correctly is the momentary appearance of the hourglass There is no message indicating successful completion 3 Connect ProScan II to PC using supplied USB cable 20 Prior Scientific 4 Switch
53. ommands Problem My stage will execute the first command sent but for each subsequent command the controller returns an error message E 5 Suggestions Make sure that your computer is not sending an extra line feed command at the end of each command being sent If you are using HyperTerminal check that the set up is correct using Appendix C 80 Prior Scientific Problem The focus motor turns but the microscope fine focus knob does not Suggestions The focus drive may not be properly positioned against the fine focus knob Loosen the knurled screw that holds the focus motor and press it gently against the fine focus knob While pressing re tighten the knurled screw Note If the fine focus knob on the microscope is difficult to turn or the movement rough then the focus drive may not function until the microscope is properly adjusted Problem The focus drive does not repeat to the same Z position every time Suggestions Check that the Z backlash routine is turned on Refer to the Command Set for command BLZH For upright microscopes check that the focus motor is mounted on the right For inverted microscopes check that the focus motor is mounted on the left If the focus drive cannot be mounted on the correct side of the microscope refer to the command set and use command ZD to reverse the rotation of the motor Problem Filter Wheel will not operate Suggestions Make sure t
54. onfigure itself to work when powered up Raster Pattern A programmed movement where the stage moves a set number of steps across a sample in the X axis and then moves back to its starting position before moving in the Y axis RS 232 A communication standard which specifies electrical mechanical and functional characteristics for serial binary communication circuits in a point to point link Commands from a computer s COM port travel to the controller via RS 232 Serial Control A type of information transfer where the bits are handled sequentially Servo Motor A motor that rotates due to the interaction of the stator field and armature field Servo motors require feedback loops 1 e encoders Shutter A device which can be opened or closed to enable or prevent the passage of light between an illumination device and the microscope or between the microscope and a detector such as a camera Snake Pattern A programmed movement where the stage moves a set number of steps across in the X axis and then moves in the Y axis The stage then moves back to its original X position This movement is repeated until the complete area of interest has been scanned creating a snake like pattern across the sample SOAK command A set of commands which can be entered and started in a block These non commands will be continuously performed in a loop until the 1 or k command is entered or the power is cycled Solid Frame S
55. or axis a RIS Restore Index of Stage This command is only effective if the SIS command has been used on installation This Command can be used at any time and will re synchronise the stage and controller position should the stage have been manually moved when the controller was off The stage will hit limits and then return to the position stored by the controller prior to the last power down If the stage has not been manually moved this command will not normally be needed 44 Prior Scientific SAS a l0 Sets the maximum stage acceleration to a Range is 1 to 100 SAS la Report current stage acceleration SCS 0 Sets the current stage S curve value This is the rate of change of acceleration during the transition from stationary until the pa reaches the full acceleration set by SAS Range of c is 1 to Ex ue 5 Set Index of Stage This command would normally only be used on first installation of the system The stage moves to limits and sets absolute position to 0 0 The controller will always remember this internally as zero even with subsequent uses of Z and P x y command LO om Sets the current Stage x y maximum speed to m Range is 1 to Nowe Report the current Stage x y maximum speed setting m ba None Text string Prints information about the currently connected stage There are 250 microsteps per full step of the motor The final line of information is always a line saying END This allows for the addition of extr
56. puts one Byte The matching female part to be used with this header is an Industry standard ribbon cable socket with centre bump polarisation to ensure correct orientation manufactured by 3M part number 3421 6620 Thomas and Betts part number 609 2041 etc The TTL outputs use a 74HCT374 buffer IC and the TTL inputs input to a 74HCT541 5 Volts is available from pins 1 and 2 shorted together to share the current for TTL power to a limited amount of circuitry Maximum current 100mA OV is the zero volts of the electronics inside the controller which is also chassis ground K2 Pin allocation 1 5V OUT aaa pA Aa A MEME 72 Prior Scientific 6 17 TTL Command set Writes to TTL port DCBA where DCBA are Hexadecimal numbers assigned as follows A TTL OUT least significant 4 bits H129 had 8 TTL IN OUT bits B TTL OUT most significant 4 bits H130 only has 4 TTL IN OUT bits C TTL IN least significant 4 bits B and D are ignored D TTL IN most significant 4 bits Since TTL IN are inputs to the controller only i e cannot be written to C and D should both be 0 any other value is ignored Acceptable formats are TTL 000E writes TTL high to all 4 TTL OUT bits except TTL OUT 0 TTL E as above DCBA BA is actual status of 4 Write Bits DC is TTL levels of 4 Input bits leading zeros may be omitted n m Where n is number 0 to 3 and is the data bit number of TTL_OUT see table above m is 0 or 1 and s
57. r Wheels Male to Male C mount adapter Filter Wheel Adapter for Leica pre DM Series Filter Wheel Adapter for Leica DM Series Filter Wheel Adapter for Nikon Eclipse Series Filter Wheel Adapter for Olympus BH Series Filter Wheel Adapter for Olympus BX IX and AX Series Filter Wheel Adapter for Zeiss Axio Series Filter Wheel Adapter for Zeiss pre Axio Series Mounting Plate Converts HF115 into HF110 Cover Plate Converts HF200 into HF202 Cover Plate Converts HF201 into HF204 Note Make sure that all of the components that should be included with your ProScan System have been supplied If parts are missing please contact your local Prior Dealer TG Prior Scientific Installation Section 4 ProScan II 17 18 Prior Scientific Installation 4 1 Removing an Existing Stage To avoid damage to the optics when removing an existing stage ensure that it is moved as far away from the objectives as possible and that the condenser is also clear Removal of the stage is normally a straightforward procedure in most cases just by the removal of fixing screws or the loosening of a clamp screw 4 2 Fitting the ProScan Stage The Prior ProScan stage is supplied with the correct base plate to suit the microscope specified Place the stage onto the microscope stage mount and attach using the fixing screws or clamping screw supplied Confirm that the ProScan controller unit is switched off before connecting the stage to the co
58. r to install Click Next Select Cyprus USB HID gt COM device Click Next Ignore warning message and click continue then select Finish 9 Cyprus USB HID gt COM n will now have been added to the list of Ports COM and LPT Note this will only be the case if the ProScan II is still connected to USB and switched on ProScan II 21 User will then be able to communicate to the ProScan II using designated connection COM n e g COM3 This can be confirmed using a terminal emulation program such as HyperTerminal If HyperTerminal is used none of the default settings within this application will need to be modified 4 5 Focus Drive Installation Clamp screw Focus adapter Figure 4 4 For installation procedure for Zeiss Axio range H122X200 H122AXIO and H122AXIE Leica DML range H122LB and direct coupling models see specific instruction sheets The following instructions refer to the standard split sleeve mounting 1 Loosen the clamp screw on the focus motor assembly and remove the focus motor from the focus adapter See fig 4 4 2 Loosen the 3 socket set screws around the periphery of the focus adapter using a 2mm Allen wrench until the focus sleeve is able to fit inside the adapter Note that it is important to insert the sleeve in the correct orientation with the lip furthest inside the adapter the chamfered edge of the sleeve will be inserted first Note the orientation of the sleeve as it ha
59. ration as follows CS152Z Z only digipot CS152V2 2 axis joystick CS152V3 3 axis joystick CS152DP 2 axis joystick with digipot for Z axis CS152EF 2 axis joystick with digipot for Z axis with fire button The CS152Z is used for the control of a system equipped with only a Z axis It has a large round digipot control which provides fine control of the focus motor A button is provided to change the focus motor speed range and there are two further buttons which can be used for coarse focus control by driving the focus up or down while the button is pressed Joysticks may be fitted with up to 3 buttons 2 long hot keys on either side of the joystick and a fire button on top of the joystick In addition there are 2 small sliding buttons one to the side and one below the joystick These provide spring loaded tension to the joystick which ensures it always returns to its vertical off position Ensure these sliding buttons are fully home and the joystick is held vertically before turning the system on The joystick can be used to control the speed and direction of the stage A small deflection of the joystick results in slow stage movement while a large deflection provides high speed movement The direction of movement of the joystick normally produces a corresponding movement of the stage For example moving the joystick to the left will move the stage left Please note however that there are commands available to rever
60. rcentage in range 1 to 100 Reports value of O allowing for joystick speed buttons effect 1f the button speed is Y and O is set to 50 the returned value will be 25 Reports absolute position of x y and z axes This can be used whilst any axis is moving to give position on the fly Note lt CR gt Carriage return only will also return position Sets absolute position of x y and z axis No axis can be moving for this command to work If there is a linear encoder fitted on the Z axis the position can only be set when the current position is within the encoder range and it has previously been at some lower position If neither of these conditions has been met an error will be reported 43 Ps Noe Reports position of Stage only x and y Sets Absolute position of x and y axis No axis can be moving for this command to work Reports position of x only gt hk Absolute position of axis No axis can be moving for this EN to work u Reports position of y only Sets Absolute position of y axis No axis can be moving for this command to work R None R O Moves Right by u steps as defined by X command R x R Moves Right by x steps Moves Right by x steps RES Ji Sets the desired resolution for the stage s is X and Y axes r can be a non integer number setting the resolution for the axis in units of microns e g RES s 1 0 Resolution set to 1 0 micron RES la Retums resolution f
61. rent filter wheel w S Curve setting Range is 1 to 100 Report the current filter wheel S Curve setting c Sets the current filter wheel w maximum speed to m in units of c Report the current filter wheel w maximum speed setting m Range is 1 to 100 52 Prior Scientific 6 6 Shutter Commands s c If shutter s is not fitted E 20 will be returned LIT S C If shutter s is not fitted E 20 will be returned Shutter Text string ProScan II Opens or closes the shutter s value 1 2 or 3 if c is 0 the shutter is opened 1 it is closed The optional argument t is used to open close the shutter for a time t milliseconds First parameter is a zero Subsequent parameters define the startup state of shutters s1 s2 and s3 respectively if s1 is O then shutterl is opened 1 it is closed etc Default state is all shutters closed Returns status c of shutter s Prints information about shutter s s is a value between 1 and 3 The information end is always a line saying END This allows for the addition of extra fields of information without effecting application software Users should always read lines until the END is seen in order to maintain compatibility Example SHUTTER 1 NORMAL DEFAULT_STATE CLOSED END 53 6 7 Lumen Pro Commands Reports a the output of light from the shutter in The command automatically locates LGG_SHUTTER Error 20 reported if no shutter detect
62. rovides positional information for either an XY stage or the focus drive assembly Encoders can either be rotary or linear Encoder Feedback The signal given from an encoder This signal can be displayed as in an open loop encoder system or fed back through the controller to provide closed loop control Filter A device typically made of glass or plastic and mounted in a filter wheel that is used to alter the properties of light emitted from a microscope s light source Filter Wheel A motorized turret that holds and positions optical filters in the light path of a microscope ProScan II 89 Fine Focus Knob The small knob on the side of the microscope that moves the stage up and down small amounts with relatively large movements Typically 100 microns per revolution of the fine focus knob Flash Memory Capability The ability of the Prior H130 controller to download new software without requiring an EPROM change This ability is analogous to that of a solid state hard drive Focus Drive A motor and adapter assembly that typically mounts to the coarse focus knob of a microscope and drives the fine focus knob Hyperterminal A terminal emulation program provided with Windows Operating Systems Instructions for setting up Hyperterminal to communicate with the Prior controller are located in the appendices Imaging Packages The general class of computer software including stereology and image analysis which utili
63. s b 1 Enables ALL encoders Returns whether the individual axis have encoder enabled or disabled Axis S X Y Z A b 0 Disables encoder specified by Axis b 1 Enables encoder specified by Axis SENCODER As above Behaves like the ENCODER commands above except that it forces the encoder and motor positions to be the same when enabling and disabling encoder function ProScan II 69 EN Sets the encoder window n for the Axis specified This can prevent excessive hunting as controller tries to close the position loop Returns the allowable encoder window for the Axis in encoder counts for determining when position acquired SERVO none n Reports back as a decimal number the axes that have servo operation enabled For example 4 means Z axis servo enabled others disabled SERVO b 0 stage moves to destination position as read from encoders and stops There is no servo action therefore the stage can drift from destination position due to external mechanical and thermal forces b 1 Stage continuously reads position from encoders even when stationary and corrects for any drift SERVO Axis 0 1 Returns whether the individual axis have servo enabled or disabled Axis S X Y Z A SERVO Axis b b 0 Disables servo specified by Axis b 1 Enables servo specified by Axis Sets a wndow of n encoder counts about the current stage position to prevent excessive hunting when servo enabled LIE prevent excessive
64. s a recess around its outer surface which will hold the sleeve in when the setscrews are tightened This recess must line up with the tips of the socket set screws See fig 4 4 22 Prior Scientific 3 With the sleeve in place tighten the 3 socket set screws in sequence until they all just touch the sleeve ensuring that the split in the sleeve does not line up with any of the set screw positions DO NOT TIGHTEN UP ANY OF THE SETSCREWS AT THIS STAGE 4 Push the adapter onto the preferred coarse knob of the microscope as far as it will go The controller is factory configured to drive the focus motor in the correct direction when mounted to the right hand side of an upright microscope If the left hand coarse control knob is preferred by the user or the focus drive is to be mounted on an inverted microscope the motor direction can be reversed by using a PC with a terminal emulation program e g HyperTerminal and changing the settings of the ZD command see section 6 via RS232 communication The inside fitting diameter of the sleeve is designed to be slightly larger than the coarse knob provided the setscrews have not been tightened and are compressing the sleeve 5 While holding the adapter in place tighten the set screws in sequence only enough to secure the unit onto the coarse focus knob The focus knob will have to be rotated to gain access to all of the screws 6 Check that the unit has been tightened sufficiently by tak
65. se the direction of the stage on one or both axes see Advanced Operation The CS152DP and CS152EF joysticks are fitted with a round digipot control on the side of the joystick box This is used for fine focus control The digipot is a rotary encoder which comprises a disc with radial lines and 2 LED detectors As the disc is rotated by the knob the encoder generates two square wave signals One controls the amount of movement while the other monitors the direction of movement This provides precise positioning of the fine focus knob on the microscope while the system continuously keeps track of the actual position The ProScan controller offers a specific command which can be used to change the function of any of the buttons on the joystick ProScan II 61 The command has the following format Command Arguments Response including lt cr gt BUTTON bf 0 There are 4 possible conditions identified by b b 0 Button released i e changing to a state where no button is pressed b 1 Right button pressed b 2 Left button pressed b 3 Fire button pressed on top of joystick Pressing more than one button simultaneously will not register a command f defines what action the controller does when it first detects a new state given by b above f 0 1 2 3 4 2 5 6 7 8 9 f 10 f 11 f 12 f 13 f 14 f 15 Default Button Function Z motor velocity stop only used in conjunction with f 2 and
66. shutter A move to filter position 4 wait 500msecs open shutter A move to filter position 1 start the soak Note MACRO and SOAK can only be used in Standard Mode COMP 0 ProScan II 7 6 2 General Commands 38 Reports information about the peripherals currently connected to the controller e g DRIVE CHIPS 10011 means Z and 4 axis chips missing SHUTTERS 110 means shutter 1 not connected The final line of information is always a line saying END This allows for the addition of extra fields of information without effecting application software Users should always read lines in until the END is seen A typical response is shown below PROSCAN INFORMATION DSP_1 IS 4 AXIS STEPPER VERSION 2 7 DSP_2 IS 2 AXIS STEPPER VERSION 2 7 DRIVE CHIPS 010111 F2 Fl AZ Y X 0 Not Fitted JOYSTICK ACTIVE STAGE H101 2 FOCUS NORMAL FILTER 1 NONE FILTER_2 HF110 10 SHUTTERS 001 S3 52 S1 0 Not Fitted AUTOFOCUS FITTED VIDEO NONE END Reports whether any limit switch has been hit since the last call of the command Nm is a two digit Hex number one Byte which converted to binary is as follows D07 D06 DOS D04 D03 D02 DO 0 4 44 Z Z Y Y X 4X eg 05 means Y and X have been hit Reading this status clears it Prior Scientific a Decimal number COMP None 0 Std 1 Comp ProScan II Reports status as a decimal number and gives motion status of any axis of t
67. tage A stage where there is no path for transmitted illumination i e the stage plates are solid without holes in them Stage Travel The overall length in X and Y that a stage can move Standard Commands The set of commands in the Prior Scientific H130 which the controller is allowed to queue These commands are enabled by the Comp 0 command ProScan II 91 Step Size The movement in microns initiated by either the L R F B U or D command This is also the movement of 1 pulse or the minimum stage movement Stepper Motor A motor which when current is applied generates a holding torque The motor is rotated by switching the coils on and off The stepper motors in Prior stages and focus motors generally have 200 steps per revolution which is then micro stepped to 50 000 micro steps per revolution TTL Transistor to Transistor Logic An integrated circuit with its inputs and outputs directly tied to transistors Inputs and outputs are low voltage lt 1 VDC and high voltage gt 3 VDC Transmitted Illumination Light which passes through the object Upright Microscope A microscope that views the object from above X Theta Stage A stage with motion in both the X direction and Rotational or Theta Direction XYZ The term used to describe the axes of a microscope that move left right X front back Y and up down Z 92 Prior Scientific Replacement Parts Section 10 ProScan II 93 94 Prior Scientific
68. the appropriate axis None luv Reports the current step size u and v in x and y for the stage Sets the current step size for the stage Sets the direction of the X axis move mechanical with respect to the software move Use this command if L command moves stage mechanically right C 1 or 1 Sets the direction of the Y axis move mechanical with respect to the software move Use this command if F command moves the stage mechanically backwards C 1 or 1 Iz Noe l0 Sets the stage and focus position to ZERO 0 0 0 16 Prior Scientific 6 4 Z axis Commands IR Move to absolute Move to absolute positionz 2 Sets the z axis backlash value for z axis move commands sent via the serial port not joystick moves to b s 1 enables backlash s disables backlash b is the number of microsteps per motor 0 There are 50 000 microsteps per revolution of the motor on a standard ProScan system EE IM Enables Disables the z axis backlash s 1 enables backlash s 0 disables backlash aa a es acon port see above In compatibility mode only s returned BLZJ Sets the z axis backlash value for joystick digipot moves to b in microsteps s 1 enables backlash s 0 disables backlash BLZJ Enables Disables the z axis backlash for joystick digipot control s 1 enables backlash s 0 disables backlash BLZJ None Reports s and b values for z axis see above In compatibility mode o
69. tware dealer Check communication by monitoring the TX transmit LED this will flash rapidly when data is being transmitted by the controller and the RX receive LED this flashes rapidly when receiving data from computer Close down the Image Analysis Software ProScan II 79 Set up and run HyperTerminal Windows Users see Appendix C Try several basic commands while in HyperTerminal to determine if the stage responds correctly 1f so the controller is functioning properly At this point consult the supplier of the Image Analysis Software If the stage does not respond to the commands contact your local Prior dealer for further advice Note Before running commands using HyperTerminal make sure that your microscope objectives and any other objects that may impede the motion of the stage are moved out of the way to avoid damage Problem System will not respond to remote RS232 commands Suggestions Set Up HyperTerminal see Appendix C Check that the RS232 cable is plugged into the RS232 1 port in the back panel of the ProScan Controller Press Enter on your computer keyboard several times If the TX and RX lights on the front of the controller blink and the system does not respond then there is an internal problem with the controller Return the unit to your local Prior Dealer If the TX and RX lights do not blink on and off then there is a problem with the computer sending the c
70. uck Pod with vacuum Accessories Focus Drive and Adapter Focus Drive and Adaptor with Rotary Encoder Focus Drive Complete for Zeiss AxioSkop 2 AxioPlan 2 imaging ie Focus Drive and Adapter for Zeiss AxioSkop AxioPlan AxioPlan 2 amp AxioVert 100 Focus Drive and Adapter encoded for Zeiss AxioSkop AxioPlan AxioPlan 2 amp AxioVert 100 Focus Drive and Adapter for AxioVert 200 with Encoders Filter Wheel 25mm Filters Filter Wheel 32mm Filters Shutter for use with 25mm Filter Wheel to be mounted to filter wheel Shutter for use with 32mm Filter Wheel to be mounted to filter wheel Shutter 25mm aperture stand alone Shutter 32mm aperture stand alone RS232 cable for PC 9 or 25 pin RS232 cable for Macintosh Solid Couple Adapter for Focus Drive on Olympus Nikon Solid Couple Adapter for Focus Drive on Leica HWC105 H246 H356 H353 H225 H232 H148 H149 H149N HWC20S HWC20V HWC116 H233 H221 HWC30S HWC30V HWC112 H122 H122E H122AX2 H122AXIO H122AXIE H122X200 HF110 HF108 HF200 HF201 HF202 HF204 H276K H277 H122KON H122KLC 96 Prior Scientific Keypad for Manual Control of 2 Filter Wheels and 3 Shutters Male to Male C mount adapter Filter Wheel Adapter for Leica pre DM Series Filter Wheel Adapter for Leica DM Series Filter Wheel Adapter for Nikon Eclipse Series Filter Wheel Adapter for Olympus BH Series Filter Wheel Adapter for Olympus BX IX and AX Series Filter Wheel Adapter for Zeiss A
71. uracy or repeatability range The following example compares RMS and 3 Sigma Accuracy Consider a stage at the Home position which is 1 micron in the X axis away from the stage zero position The stage can be cycled through a series of moves which take the stage away from Home and then return to Home at which point the actual position in the X axis is measured After 14 cycles the following data may be collected 1 8 1 5 0 1 1 7 1 8 Lil 1 8 0 9 1 9 2 0 0 0 1 5 0 9 1 8 The 3 sigma accuracy for these moves is 1 0 microns while the RMS accuracy is ProScan 2 0 7 microns The stage can be shown mathematically to have 2 different accuracies However the data shows that 1 2 of the measured values fall outside the 0 7 RMS accuracy range while all the data fall within the 3 Sigma accuracy range Calculating accuracy using the RMS method exaggerates the accuracy of a stage 2 1 Step Motors and Resolution The Prior ProScan stages and focus drives use high precision step motors Generally the step motors used in Prior stages products are 1 8 degree 200 steps per revolution motors The ProScan controllers provide a bipolar chopper drive to the motors which allow for maximum torque stabilization smoothness and performance The motors receive pulses from the controller which in turn causes them to rotate If the motors are operated in a full step mode one pulse from the controller will
72. utofocus button on top of the joystick shaft 30 Prior Scientific 5 3 Using the Z Axis Digipot CS152Z This device is normally used for ProScan systems where motorized focus is required but a motorized stage is not It provides control of the focus motor only The digipot control drives the focus motor directly Rotating this device in opposite directions will reverse the direction of movement of the focus motor The speed control button can be used to change the maximum speed of the focus motor when controlled from the digipot Press once to reduce the speed to 50 of maximum a second time for 25 of maximum and a third time to return to 100 of maximum This cycle can be repeated by continuing to press the button Two other buttons are provided which allow the focus motor to be driven Up or Down by pressing and holding the appropriate button This feature allows for rapid positioning over larger distances 5 4 Using the Filter Wheel Keypad CS100K The Filter Wheel Keypad CS100K provides control of up to 2 filter wheels and 3 shutters at the press of a button It communicates with the ProScan controller via the RS232 2 port located on the rear panel see fig 4 3 Ensure that the controller is switched off before connecting the keypad Switching on the controller automatically initiates a homing routine and either the FW1 or FW2 green LED will be illuminated Filter wheel position 1 on th
73. xio Series Filter Wheel Adapter for Zeiss pre Axio Series Dual Slide in Filter Holder for Filter Wheels Mounting Plate Converts HF115 into HF110 Cover Plate Converts HF200 into HF202 Cover Plate Converts HF201 into HF204 Filter Wheel Stand ProScan II CS100K HF207 HF210 HF215 HF220 HF230 HF235 HF240 HF245 HF205 HF535 HF559 HF568 HF310 7 98 Prior Scientific Returns and Repatrs Section 11 ProScan II 99 100 Prior Scientific Returns and Repairs Should you experience problems with your ProScan System and want to send it back for service warranty or otherwise a Return Material Authorisation RMA number must be obtained from the appropriate Prior Scientific office before returning any equipment For North and South America contact Prior Scientific Inc and for the rest of the world call Prior Scientific Instruments Limited on the telephone numbers shown below Prior Scientific Instruments Limited Unit 4 Wilbraham Road Fulbourn Cambridge England CB1 5ET Telephone 01223 881711 FAX 01223 881710 email uksales prior com ProScan II Prior Scientific Inc 80 Reservoir Park Drive Rockland MA 02370 1062 USA Telephone 781 878 8442 FAX 781 878 8736 email info prior com 101 102 Prior Scientific Appendices Section 12 ProScan II 103 104 Prior Scientific Appendices Appendix A 12 1 How to Run HyperTerminal Instructions for using Windows Terminal
74. ze motorized stages and or focus drives Incident Illumination Light which falls on the object from any direction typically refers to reflected light applications Inverted Microscope A microscope that views the object from below The objectives are underneath the stage Joystick A device which allows manual movement of a motorized stage and focus without using RS232 commands Legacy Commands The set of RS232 commands that are common between the H127 H128 and H130 Prior Motor Controllers See also compatibility commands Linear Scales See Encoders MACRO A set of commands that can be entered and started in a block by the use of the MACRO command Mechanical Stage The manually operated X Y positioning table that comes as standard with most microscopes Motorized Stage An XY positioning table typically mounted on a microscope that is moved via stepper or servo motors and their corresponding controller Open Frame Stage A stage that allows for transmitted illumination via holes in the X and Y plates 90 Prior Scientific Open Loop System A control system that has no means of comparing the output with the input for control purposes Open loop stage systems rely on the controller to send the proper amount of pulses to the motor to achieve the required movement Plug Play Facility The ability of the Prior Scientific controller to recognize which components accessories are connected and to auto c

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