INTRACRANIAL SELF-STIMULATION
Contents
1. 12 Chapter e 12 Understanding the MED State Notation 12 Chapter 5 oueisneuaa2523233433883583485 E SERERE AR SR RAS AR AR NR SA AR E MAR ERR EREREAE 17 Understanding the Data eee nenne 17 Sample Data vene us ens ea MAR x RR RR Ar 17 MED ASSOCIATES INC 1 55 PROCEDURES CHAPTER 1 Introduction The purpose of this manual is to give an explanation of the MED State Notation Procedures that comprise the SOF 700RA 5 Intracranial Self Stimulation 1 55 Procedures The files in this package can be found on the disk provided by MED Associates Inc These procedures are intended to be run in MED Associates MED PC IV software The latest version of MED PC IV gives researchers the ability to use pre programmed procedures such as these to make hardware control and data collection easy These pre programmed procedures can also be modified to meet the evolving demands of a research protocol Again it is the intent of this manual to explain exactly what these procedures implement and provide guidance into how to interpret what the program code achieves in order to let the user determine how to modify them to match their research protocol demands The manual provides s2. NEN Pulse Width 2 200 00 5 Step Size 10 00 Pulse Width 1 200 00 Pulse Amp 1 80 00 Pulse Delay 100 00 SHOWS 6_10 Pulse Amp 2 80 00 Frequency 100 00 Pulse Train Dur 500 00 52 80 00 Node 1 00 SHOWS 11 15 SHOWS 16 20 12 18 2008 11 16 51 AM Eie for toading Boxes o Session MED ASSOCIATES INC ICSS PROCEDURES Viewing Changing Variable Values Before a start command has been issued any variable may be changed on the MED PC IV run time screen Simply highlight the value to change and then enter the new value Once a session is in progress change variables by selecting Configure Change Variables or click the 4th tool bar item AX In the lower left hand corner of the Change Variables window find the Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded to all of the selected boxes Figure 3 11 Changing Variables Screen Displaying Variables from Box 1 A Anay 0000 C Array D Array o Mes E G H J Yars 0 000 0 000 0 000 0 000 0 000 L M N 0 000 0 000 0 000 0 000 0 000 Refresh P Array ne Ape Zm T Array Issue Y a 0 000 0 000 0 000 0 000 0 000 ase 2 0 000 x Help Display Data from Box Additional Boxes to Update B from Box 1 1 0 000 A Select All Deselect All
3. performing the steps manually Once the commands are in the macro it is easy to create a number of macros with the macro editor The following example illustrates the process of loading Box 1 and changing the Step Size to 30 To begin open MED PC IV and going directly to the run time screen Close the load wizard if present Before loading or opening the procedure click Macro on the main menu and select Turn On Macro Recorder or click the 8th tool bar item with the cassette tape icon on top A note on the bottom of the display indicates that the recorder is running Open Detection mpc by clicking Files Open Session Change the variables using any of the methods described above When all settings have been made turn the recorder off again by using the main menu or tool bar Save the macro with distinctive The example in Figure 3 14 was named Detection_StepSize_30 mac since the Step Size was changed to 30 Figure 3 14 Detection StepSize 30 mac Detection StepSize 30 MAC Notepad File Edit Format View Help LOAD BOX 1 SUBJ 0 EXPT 0 GROUP 0 PROGRAM DETECTION SET step size uA VALUE 30 MAINBOX 1 BOXES 1 Once this macro is built use the macro editor to make simple changes such as replacing reward or correct lever values Review the Help file on screen or the MED PC IV User s Manual for more information on macros and the features offered A START command or message box followed by a START command coul
4. 000 000 000 000 nn B Response Latency Counter 000 Subscript for the Master Data Array 000 000 000 000 000 000 000 000 000 MED ASSOCIATES INC ICSS PROCEDURES 105 15 205 10 15 20 254 30 35s 40 45 50 553 60 65 70 75 80 85 90 95 100 105 T1054 115 120 125 130 135 000 000 000 000 0 000 m 10 200 000 000 000 860 000 000 000 000 000 000 000 2590 000 060 000 290 000 750 000 000 000 000 000 000 000 230 000 160 000 240 000 000 000 10500 73 80 000 000 000 000 000 190 000 000 000 000 000 760 000 000 000 180 000 380 000 540 000 860 000 140 000 000 000 000 000 410 000 460 600 000 000 000 200 100 000 000 000 000 000 070 000 980 000 000 000 000 000 000 000 000 000 350 000 810 000 000 000 000 000 000 000 000 000 000 000 240 390 000 000 000 80 500 000 000 000 000 790 000 000 000 000 000 000 000 000 000 670
5. Train Duration Duration of the stimulation in milliseconds 500 ms This variable used in Detection mpc program only This is the reward stimulus Pulse Amplitude 2 duration The value remains constant for S2 during testing Only Pulse Amplitude 1 and Pulse Amplitude 2 will change during testing References Kornetsky Conan and Bain George 1990 Brain Stimulation Reward A Model for Drug Induced Euphoria Modern Methods in Pharmacology Volume 6 Testing and Evaluation of Drugs of Abuse Wiley Liss Inc pp 211 231 Bird Michael and Kornetsky Conan 1990 Dissociation of the Attentional and Motivational Effects of Pimozide on the Threshold for Rewarding Brain Stimulation Neuropsychopharmacology Volume 3 pp 33 40 2 Getting Started Software Installation Please refer to the MED PC IV User s Manual for a complete guide to installing the MED PC IV software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility To install the ICSS Procedures insert the CD into the CD ROM drive and click Install the Intracranial Self Stimulation Software The 1 55 procedures are copied into the C MMED PC folder Backing Up the Software Med Associates strongly encourages creating backup copies of the programs in case of disk failure Having copies of the original programs may
6. the lower limit and switch to an Ascending trial block The problem is that the animal is not responding because he doesn t like the stimulation not because he can t feel it The program switching to Ascending trial blocks and increasing the amplitude will only make the problem worse The program will end after four reversals i e A change from a Descending trial block to a Ascending trial block or a change from a Ascending trial block to a Descending trial block Detection mpc This procedure is a variation on the reward procedure in which the reward stimulus remains constant and only the free stimulus changes while testing thresholds This procedure is used in training a test subject It also determines the minimum stimulation amplitude necessary to produce a response by the test subject Independent Variables Independent Description Range Default Variable 9 Value The amount that the current will be Step Size increased or decreased in uA during 10 uA testing Pulse Width 1 The width of Pulse 1 in microseconds 60 32 000 us 200 us Pulse Amplitude 1 The amplitude of Pulse 1 in microamps 1 1 000 uA 80 uA The delay between Pulse 1 and Pulse 2 in Pulse Delay microseconds 60 32 000 us 100 us Pulse Width 2 The width of Pulse 2 in microseconds 60 32 000 us 200 us Pulse Amplitude 2 The amplitude of Pulse 2 in microamps 1 1 000 uA 80 uA Frequency Frequency in hertz 1 2 000 Hz 100 Hz Pulse
7. 000 440 000 790 000 420 000 000 000 000 000 000 000 190 000 600 000 160 000 000 000 18 100 80 000 000 000 000 430 000 000 000 000 000 000 000 940 000 070 000 000 000 190 000 270 000 360 000 000 000 560 000 000 000 4390 000 460 000 000 000 Most of these values are used for the control flow of the program At the end of the program the values that still mean something are A 3 Number of Reversals A 11 Total Cluster Resp A 12 Total ITI Resp A 13 Total Responses A 14 Total Free Stimuli The C Array contains the main data that is saved from the program Data is saved in blocks of 10 Here is what the data means C J 0 Descending Trial Block 1 Ascending Trial Block 1 Stim Amplitude Value this Trial Block 2 Reward Stims this Trial Block C J 3 Response Latency for 1st Trial C J 4 Response Latency for 2nd Trial C J 5 Response Latency for 3rd Tria C J 6 Response Latency for 4th Tria C J 7 Response Latency for 5th Tria C J 8 Number of Cluster Responses this Trial Block C J 9 Number of ITI Responses this Trial Block The D Array is used to save the response latency for each trial 1 5 The values are saved to the C Array at the end of each Trial Block The P Array contains the start
8. A 11 SX Count Cluster Responses 2 83 53 R Wheel ADD A 8 12 gt SX Count ITI Responses 21 gt 81 State Set 4 counts the total number of Responses III I III I E E E E III III III I I I I I E COUNT TOTAL RESPONSES I TORR IRR TOR TOR IR RK RK RR RRR RE 5 8 4 51 START gt 62 82 R Wheel ADD A 13 gt SX Count Total Responses State Set 5 updates the display with the most current information for the running trial E E III III I III III I I II I e DATA DISPLAY I TORR IR RR TORR TORR IR OK RK RRR RRR RE 8 5 51 START gt S2 82 0 5 SHOW 1 Column 3 1 2 D A Tag A 4 3 Block 10 SHOW 4 Stim Amplitude A 16 5 Reward A 1 11 Resp Latency D A 5 SHOW 12 Cluster Resp A 7 13 ITI Resp A 8 14 Criteria Met A 0 SHOW 15 Two Criteria Met A 2 S3 83 0 1 SHOW 21 Total Cluster 11 22 Total ITI 12 23 Total Resp A 13 SHOW 24 Total Free Stimuli A 14 31 Trial A 5 32 ITI Interval A 6 1 SHOW 33 Resp Dur Free Stim A 9 gt S2 5 Understanding the Data Files Unless otherwise specified data will be saved to C MED PC IV DATA Data can be saved manually by selecting FILE SAVE DATA MANUALLY or FILE SAVE DATA FLUSH The file name that is used to save the data
9. A 6 T SET C J A 4 C J 1 A 16 SET C J 2 A 1 C J 3 D 1 SET C J 4 D 2 C J 5 D 3 SET C J 6 D 4 C J 7 D 5 SET C J 8 7 C J 9 A 8 SET C J 10 987 987 PRINT gt S20 820 End Procedure 1 gt STOPABORTFLUSH State Set 2 records the latency to the response during the trial III I III III ICIS III I III III I e I I I RESPONSE LATENCY I IR IRR TOR TORR IR OK RK RK KR RK RK 5 5 2 Record Response Latency in Array D until End of 5 Trial Block ST when transferred to Print Array S S 1 S16 amp S17 21 82 Start Clock with Occurance of Free Stimulus S2 R Wheel SET D A 5 B B 0 gt 81 23 SET B 0 81 No Response Made 0 01 SET B B 0 01 SX State Set 3 counts the Cluster Responses and the number of Responses during the ITI Cluster Responses is the first response that happens during the trial and all of the responses during the first 2 seconds of the ITI E E E E E I I I COUNT CLUSTER ITI RESPONSES RR IRR I IRR TORR TORR IR RK RK KR RK RK S S 3 Counters transferred to Print Array and Reset at End of 51 each 5 Trial Block See 8 5 1 S16 Z2 gt S2 52 R Wheel ADD A 7
10. Descending trial block tag Starts at NA State Sets in the Response Latency counter Resolution 0 01 sec D Response Latencies for each Trial Block Subscript 5 NJ Subscript for the Master Data Array C i e Set C J A 4 P Stimulator Parameters LOW LIMIT DEFAULT Set at 10 microamperes in S S 1 S8 HIGH LIMIT DEFAULT Set at 200 microamperes in S S 1 S13 T List of Available ITI Intervals Mean 15 seconds DIM A 20 DIM C 500 DIM D 10 LIST P 10 0 200 80 100 200 80 100 500 80 LIST T 7 5 9 10 5 12 13 5 15 16 5 18 19 5 21 22 5 Z Pulses Used in this Program Z1 Signal Start of a Free Stimulus Z2 Signal Start of ITI Period Z3 Signal for no Response Made During 7 5 Following Free Stim PRINTVARS PRINTOPTIONS 80 FORMFEEDS PRINTFORMAT 6 2 IRR TORR TORR KR RK KE Detection Schedule S1 Set Default Values N Step Size Pulse Width 1 Pulse Amplitude 1 Pulse Delay 100us Pulse Width 2 Pulse Amplitude 2 Frequency Pulse Train Duration Pulse Amplitude 2 for S2 80uA I I heck ke RR RRR ke ke kk RK State Set 1 is the main portion of the program It determines if this is an Ascending or Descending trial and whether or not the criteria h
11. I NTRACRANI AL SELF STI MULATION MED STATE NOTATION PROCEDURE DOC 128 Rev 1 1 Copyright O 2008 All Rights Reserved Med Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC 1 55 PROCEDURES TABLE OF CONTENTS Chapter 1 gm 1 mcr 1 Overview of the Procedures 2 Independent Variables ioni e bb EE ERG UE Eb EE ERR ENS 3 ubertas e E 3 Chapter 2 ooeiinissisess15235835834232322834218832835838898 08889280800 880800804 8008400 00 800800 4 Getting isimcom E 4 Software lnstallabloriszscuse ramaan bao ss vitat ce dia need etel DEED 4 Backing Up the Software eite rrr P tb abes sa bv rue 4 Chapter 3 98 038188 058 02 808A ER IR RR RARE R80 8488 0080 5 Beginning amp Running an Experiment ssssssssssese eee enne 5 Translating The MED PC IV mpc mmm 5 Using the MED PC IV Load 2 m eee nenne 6 Viewing Changing Variable 9 e DA TR RR RR AR 11 Modifying the MedState Notation
12. The value of any simple variable may be viewed from this screen by clicking an array the table and each element in that array can be viewed as shown in Figure 3 12 To change a value simply highlight and replace the value in the lower right hand box or use the up down arrows to increment by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 3 13 Change variables here as needed MED ASSOCIATES INC 1 55 PROCEDURES Figure 3 12 Displaying Array A from Box 1 Displaying Array A from Box 1 A 2 A 3 49 Named Vars 0 000 0 000 0000 _Nomed var A 8 243 0 000 0 000 0000 _ 12 13 A M a oo Befresh 17 A s 90 Issue Close Help Display Data from Box Additional Boxes to Update 0 from Box 1 1 0 000 Select All Deselect Al Figure 3 13 Displaying Named Variables from Box 1 Displaying Named Variables from Box 1 Step Size uA c Named Vars Pulse Width 1 us Pulse Amplitude 1 uA Pulse Delay Refresh Pulse Width 2 us Issue Pulse Amplitude 2 uA Frequency Hz Close Pulse tre Duration ms Help Select All Deselect All 10 lt NCCOCIAT ADIULIA rri Cc INC ccc OCE S 5 INC C55 PROCEDURES Macros The simplest way to initially create a macro is to record keyboard functions while
13. a Screen should display next as shown in Figure 3 5 The purpose of this screen is to allow annotations to be added to the data file that is produced by MED PC IV These annotations will help identify the Subject Experiment and Experiment Group upon which data was collected Comments can be added here as well and the data file can be given a customized file name to help identify it from other data files Enter the information desired and click Next Figure 3 5 Enter Experiment Data Screen sus 7 Experiment Petection 3 Group 7 Comments Subject 1 Group1 Optional Custom Filename DetSubiecti_Groupt 8 The next screen to appear is the Review Choices screen as seen in Figure 3 6 This is a method of confirming that the information received from the Box Procedure Selected is correct If it is not correct select Previous and edit the data If it is correct select Next Figure 3 6 Review Choices Screen Review the data below Box 1 N Procedure DETECTION Subject Subject_1 Experiment Detection_1 Group Group 1 Comment Amp Thres Det Subject 1 Group 1 Filename Det Subjectl Groupl Click Next to finish loading the box or Previous to correct errors MED ASSOCIATES INC 1 55 PROCEDURES The Alter Session Parameters Screen shown in Figure 3 7 is the next screen to appear and is an important screen for the researcher The Alter Session Parameters scre
14. as been met to switch to the other trial type It also contains the stimulation values and when to end the program States 3 5 are used to set up the free stimulation at the beginning of a trial and determine if this is an Ascending or Descending trial 8 8 1 S1 Turn HouseLight ON when Procedure STARTS START CLEAR 1 60 ON HouseLight SET P Node BOX A 10 1 A 16 P PAmpl S2 1 SHOW 1 Step Size P Step 2 Pulse Width 1 P PWidthl 3 Pulse Amp 1 P PAmp1 SHOW 4 Pulse Delay P PDelay 5 Pulse Width 2 P PWidth2 6 Pulse Amp 2 P PAmp2 SHOW 7 Frequency P Freqg 8 Pulse Train Dur P Dur 9 S2 P PAmpS2 SHOW 10 Node BOX SX 52 0 1 Stimulate MG P 1 2 P 3 4 5 6 7 P 8 gt 83 53 Free Stimulation Given at Start of Procedure 0 01 StimOn MG BOX 1 ADD 5 14 Z1 gt 84 84 Pause during Stimulation Note Duration of Stimulation is passed with Stimulate command parameter P 8 P Dur Reset Stimulator parameters for S2 Test for a Response during Free Stimulation 0 5 Stimulate MG P 1 P 2 9 P 4 P 5 P 9 PI7 P 81 IF A 9 1 Response NoResponse Resp gt 55 NoResponse IF A 4 0 T GF T gt S6 Descending Trial gt 811 Ascending Trial R Wheel ADD A 7 A 11 SET A 9 1 gt SX 7777 85 Response occurs during the Free Stim Pause 0 5 Deliver a Reward Sti
15. be useful in the future should modifications be made to the existing programs NCCOCIAT ADIULIA m G INC CSS PROCED S CSS PROCEDURES CHAPTER 3 Beginning amp Running an Experiment Translating The MED PC IV mpc File Programs written in MedState Notation must be translated using Trans IV before they can be executed in this application Be sure that a copy of the file being translated is present in the directory C MED PC IV MPC Open Trans IV icon and select Translation Translate and Compile Select the program s to use for the experiment and click Make Click OK to start the translator and it will automatically parse the MedState Notation and then open to a DOS screen to compile the Pascal code Depending on the speed of the computer each of these steps may not be seen If any problems are encountered during this process refer to the on screen help menu or the MED PC Version IV User s Manual or contact MED Associates Inc for assistance Figure 3 1 Trans IV Control Panel for Translating and Compiling MedState Notation Code i Specify Files to Translate Detection mpc Reward mpc Build No Trans Exclude J Cancel Help MED ASSOCIATES INC 1 55 PROCEDURES Using the MED PC IV Load Wizard MED PC IV is designed to help the researcher run an experiment by guiding selection choices through its Experiment Loading Wizard This section will describ
16. d be added to the macro it was left off here so changes could be verified before starting the procedure MED ASSOCIATES INC 1 55 PROCEDURES Modifying the MedState Notation Code Permanent changes to ICSS procedures can be made to the MedState Notation code To make the same change to the Step Size as shown above do the following Open Trans IV and select File Open to place Detection mpc into the text editor Scroll down to approximately line 97 note the line counter in the lower right hand corner of the editor to reveal the code shown in Figure 3 15 Figure 3 15 Detection StepSize 30 mpc Line 97 TRANS IV C MED PC IVWPCWDetection StepSize 30 MPC BAR S File Edit Search Options Translation Window DIM C 500 10 1 200 80 100 200 80 100 500 80 7 5 9 10 5 12 13 5 15 16 5 18 19 5 21 22 5 Z Pulses Used in this Program Z1 Signal Start of a Free Stimulus amp On LIST change 10 to a 30 and save the changes with the same or a new file name such as Detection StepSize 30 mpc Remember if creating a new mpc file name and are using a macro to load boxes the file name in the macro also must be changed Translate and compile the new or changed file as described previously and run MED PC IV Use the Change Variables screen to view confirm the new values 4 Understanding the MED State Notation Procedures The Det
17. e how to initiate the Detection mpc application however the following steps that will also apply to all other mpc procedures Open MED PC IV and the MED PC Experiment Loading Wizard s Welcome screen shown in Figure 3 2 will appear Figure 3 2 The MED PC IV Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 3 9 If the choice to continue with the Loading Wizard is made then click the Next button MED ASSOCIATES INC 1 55 PROCEDURES The Box Selection screen will appear next as shown in Figure 3 3 From this screen the researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Click Next to continue Figure 3 3 The Box Selection Screen The Select a Procedure screen appears next as seen in Figure 3 4 This is where the application to be run is selected The screen displays a list of all the currently compiled procedures Select the desired procedure and then click Next Figure 3 4 The Select a Procedure Screen X DETECTION MED ASSOCIATES INC 1 55 PROCEDURES The Enter Experiment Dat
18. ection mpc and Reward mpc programs are so similar that only the Detection mpc program are described in this chapter zero by default and gets set to the value of A 4 after data is transferred to the C Array Copyright C 2008 MED Associates All rights reserved Detection mpc Detection Procedure Discrete Trial Method Method of Limits Based on procedures for Dr Conan Kornetsky Boston Univ Sch of Med Inputs Wheel 1 Chambers with Levers will have the LeftLever connected as Input 1 The constant Wheel may be changed to LeftLever here and throughout the procedure if desired Outputs LeftStimLight 1 The only output used in this RightStimLight 2 procedure is the HouseLight Feeder 3 Output constants may be modified SonAlert 4 to match individual systems HouseLight 5 P Control Variables with Assigned Aliases Defined Var_Alias Step Size uA 0 Default 10uA Var_Alias Pulse Width 1 us P 2 Default 200us Var_Alias Pulse Amplitude 1 uA P 3 Default 80uA Var_Alias Pulse Delay us P 4 Default 100us Var_Alias Pulse Width 2 us P 5 Default 200us Var_Alias Pulse Amplitude 2 uA P 6 Default 80uA Var_Alias Frequency Hz P 7 Default 100Hz Var_Alias Pulse Train Duration ms P 8 Default 500ms Var_Alias Pulse Amplitude 2 for S2 uA P 9 Default 80uA step 0 Node 1 pwWidthl 2 PAmp1 3 pDela
19. en allows the researcher to alter the parameters by which a procedure executes The Send Start Command Screen appears next The options available on the screen vary depending upon how many boxes are described in the Hardware Configuration Figure 3 7 Alter Session Parameters Screen Step Size uA Is Pulse Width 1 us Pulse Amplitude 1 uA Pulse Delay Pulse Width 2 Pulse Amplitude 2 uA Pulse Train Duration ms Pulse Amplitude for 52 uA In this example only 1 box is described in the Hardware Configuration so Figure 3 8 will appear next If more than 1 box is in the Hardware Configuration then Figure 3 9 will appear Figure 3 8 Send Start Command Screen for Single Box Configuration MED ASSOCIATES INC 1 55 PROCEDURES Figure 3 9 Send Start Command Screen for Multiple Box Configuration In both cases Figure 3 8 and Figure 3 9 the screens are where the researcher decides to either load more boxes send a start signal to boxes that are already loaded or enter the MED PC IV run time environment without sending a start signal by selecting 1 am finished with the wizard This option results in the screen shown in Figure 3 10 Figure 3 10 The MED PC IV Run Time Screen MED PC IV File Configure View Macros Help 4 amp amp amp e Subject Experiment Group Load Program 1 0 0 0 11 16 Detection
20. et GTwoCriteriaMet SET A 2 0 ADD A 3 IF A 3 gt 4 GEndProcedure GContinueProcedure GEnd RANDD A 6 T gt S17 GCont RANDD A 6 T SET A 4 1 S16 Change to Ascending Trial GTwoCriteriaNotMet gt S8 GCriteriaNotMet SET A 0 0 A 2 0 S8 ContTrialBlock RANDD A 6 T gt S15 Add Trial Count set new ITI Value S8 Test Lower Limit and Decrease Current 0 01 IF P PAmpl1 lt 10 LowerLimit GContinue GLowerLimit ADD A 3 IF A 3 gt 4 EndProcedure GContinueProcedure End RANDD A 6 T gt 517 Cont RANDD 6 T SET 4 1 S16 Change to Ascending Trial GContinue SET P PAmp1 P PAmpl1 P Step SET P PAmp2 P PAmp2 P Step Stimulate MG P 1 P 2 3 4 5 6 7 P 81 RANDD A 6 T S16 S9 Wait for S2 Pulse Train to End if Delivered Return to S1 parameters Continue Descending Trial 0 5 Stimulate MG P 1 P 2 P 3 4 5 PI6 PI 7 P 81 gt S7 States 11 14 used to control the Ascending Trials At the end of each trial State 12 tests the following e If the number of trials in the block have been completed IF A 5 gt 5 e lf the three trials with no response criteria has been met IF A 0 gt 3 e the two consecutive blocks criteria has been met IF A 2 gt 2 lf the four reversal criteria has been met IF A 3 gt 4 State 13 tests the
21. following e Ifthe absolute lower limit for the amplitude has been reached IF P PAmp1 lt 200 If it has then an automatic reversal happens e f the four reversal criteria has been met IF A 3 gt 4 Any of these criteria may be changed by changing the appropriate IF statements in States 12 and 13 The same IF statements in States 7 and 8 must also be changed Note The number of trials in a block IF A 5 gt 5 cannot be changed without also changing how the data is saved in the C Array see cee eee ee eee ee ee eee oe Sri Start of Ascending Trial 7 5 22 Z3 gt 514 R Wheel ADD A 0 A 1 A 7 A 11 StimOn MG BOX 1 Z2 gt S14 812 0 01 5 gt 5 EndTrialBlock ContTrialBlock GEndTrialBlock IF A 0 gt 3 CriteriaMet CriteriaNotMet GCriteriaMet SET A 0 0 ADD A 2 IF A 2 gt 2 TwoCriteriaMet TwoCriteriaNotMet GTwoCriteriaMet SET A 2 0 ADD A 3 IF A 3 gt 4 EndProcedure ContinueProcedure GEnd RANDD A 6 T gt S17 GCont RANDD A 6 T SET A 4 0 S16 Change to Descending Trial GTwoCriteriaNotMet gt S13 GCriteriaNotMet SET A 0 0 2 0 S13 GContTrialBlock RANDD A 6 T S15 Add Trial Count set new ITI Value 513 Test Upper Limit and Increase Current 0 01 IF P PAmpl1 gt 200 UpperLimit Continue UpperLimit ADD A 3 IF A 3 gt 4 EndProcedure Co
22. in depends on the option that was chosen in the Hardware Configuration Utility and may also be dependent on the Subject Experiment and Group name provided in the MED PC IV load wizard Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however be sure they are always saved unformatted in case a data extraction utility such as MED PC to Excel might ever be used Data file formats are explained in detail in the MED PC IV User s Manual Sample Data File Select Annotated on the file options page during hardware installation to produce a raw data file similar to the following Data files are located in C MED PC IV Data unless an alternate path was defined during hardware installation They may be opened with note pad word pad or any word processor however make sure they are always saved unformatted in the occasion a data extraction utility such as MPC2XL is used The header information should be self explanatory Data file formats are explained in detail in the MED PC IV User s Manual File C MED PC IV DATA 2006 12 06 09h09m Subject 7 Start Date 12 06 06 End Date 12 06 06 Subject 7 Experiment 5 Group 1 Session identification information that was entered during the load wizard Box 1 Start Time 9 09 59 End Time 9 36 59 MSN Detection 000 000 000 000 000
23. ing stimulation values MED ASSOCIATES INC 1 55 PROCEDURES Ta Ors 5 10 7500 000 9000 000 10500 000 12000 000 13500 000 15000 000 16500 000 18000 000 19500 000 21000 000 22500 000 19 The T Array contains the ITI values that were used in the program The values were converted to MED Ticks once the program was loaded
24. lation again as a reward The program provides the same stimulation for five trials and then the trial block ends At the end of the trial block the program checks how many times the animal responded and received a reward stimulation In Descending trial blocks the program is looking to see how many times the animal did not respond i e the animal could not feel the stimulation If the animal does not respond three out of five times for two consecutive trial blocks then the program has found the lower limit and switches to Ascending trial blocks In Ascending trial blocks the program is looking to see how many times the animal did respond to the stimulation i e the animal could feel the stimulation If the animal does respond three out of five times for two consecutive trial blocks then the program has found the upper limit and switches to Descending trial blocks Between trial blocks the program decrements or increments the stimulation amplitude depending on the trial type by the Step Size By default the program starts with Descending trial blocks so the User should select a starting amplitude that is between the expected lower and upper stimulation values It is better to guess low than to guess high For example if the User selects a starting amplitude that is too high and the animal receives a stimulation that it does not like then it will not respond The program will see that the animal is not responding and assume that it has found
25. mulation and Terminate the Trial 0 5 ADD A 1 StimOn MG BOX 1 72 IF A 4 0 T F T gt S9 Descending Trial GF ADD A 0 S14 Ascending Trial States 6 9 are used to control the Descending Trials At the end of each trial State 7 tests the following If the number of trials in the block have been completed IF A 5 gt 5 If the three trials with no response criteria has been met IF A 0 gt 3 If the two consecutive blocks criteria has been met IF A 2 gt 2 If the four reversal criteria has been met IF A 3 gt 4 State 8 tests the following e Ifthe absolute lower limit for the amplitude has been reached IF P PAmp1 lt 10 If it has then an automatic reversal happens If the four reversal criteria has been met IF A 3 gt 4 The user may change any of these criteria by changing the appropriate IF statements in States 7 and 8 The same IF statements in States 12 and 13 must also be changed Note The number of trials in a block IF A 5 gt 5 cannot be changed without also changing how the data is saved in the C Array 56 Start of Descending Trial 7 5 ADD A 0 22 Z3 gt S9 R Wheel ADD A 1 A 7 A 11 StimOn MG BOX 1 Z2 gt S9 S7 0 01 IF A 5 gt 5 EndTrialBlock ContTrialBlock GEndTrialBlock IF A 0 gt 3 CriteriaMet CriteriaNotMet GCriteriaMet SET A 0 0 ADD A 2 IF A 2 gt 2 TwoCriteriaMet TwoCriteriaNotM
26. ntinueProcedure End RANDD A 6 T gt S17 Cont RANDD 6 T SET A 4 0 gt S16 Change to Descending Trial Continue SET P PAmp1 P PAmp1 P Step SET P PAmp2 P PAmp2 P Step Stimulate MG P 1 P 2 3 4 5 6 7 8 RANDD A 6 T gt S16 814 Wait for 82 Pulse Train to End if Delivered Return to S1 parameters Continue Ascending Trial 0 5 Stimulate MG P 1 P 2 P 4 5 6 7 P 81 gt 812 States 15 17 are used to time the Inter Trial Interval and save the data from each trial Responses during the ITI are punished by resetting the ITI 815 ITI Interval for Trials 1 4 of Trial Block R Wheel gt 815 Punish Responses in ITI by Resetting Timer A 6 T SET A 9 0 gt S3 End ITI Interval 816 ITI Interval amp Data Saving for End of Block of 5 Trials R Wheel gt S16 Punish Responses in ITI by Resetting Timer A 6 T SET C J A 17 C J 1 A 16 C J 2 A 1 C J 3 D 1 SET C J 4 D 2 C J 5 D 3 0 6 D 4 C J47 D 5 SET C J 8 A 7 9 A 8 1 0 A 7 0 A 8 0 SET J J 10 IF J 500 AbortProcedure StartNewTrialBlock GAbort PRINT gt S20 StartNew SET C J 987 987 A 5 0 SET A 16 P PAmpl1 A 17 4 SET A 9 0 ADD A 10 ZEROARRAY D gt 53 S17 Save Data from last Trial Block and Generate Printout
27. ome examples of editing and modifying the procedure s programming code The manual also defines the elements in the raw data file produced by these procedures In addition to this manual refer to the MED PC IV User s Manual for the installation of the MED Associates interface drivers the MED PC IV Software and the Delphi Compiler Also refer to the User s Manual for instructions on developing a Hardware Configuration Data file structure file saving format and other related options are also determined by the Hardware Configuration Running the Hardware Configuration software utility that accompanies MED PC IV sets the Hardware Configuration Its purpose is to assign the inputs and outputs on the interface cards in the interface cabinet for each task controlled by MED PC IV The particular type of interface card that is supplied in the interface cabinet may vary please refer to the User s Manual provided for instructions on how to configure the type of card that is in the cabinet A valid Hardware Configuration must exist in order for MED PC IV to interface correctly with the MED Associates Inc hardware This means that one should take the time to create a valid Hardware Configuration before attempting to run the procedures included in this package Should there be any problems the staff at MED Associates Inc is available to answer any questions that may arise Please e mail us at support med associates com with a detailed description of
28. the problem or desired goals so that concise and detailed information may be provided The 1 55 procedures are designed to be as easy to use as possible MED Associates Inc understands that researchers do not have the time to devote to programming and hardware design and for that reason we have undertaken that burden for you We sincerely hope that you are satisfied with the products and services we provide and look forward to meeting your future experimental needs as your research program evolves Overview of the Procedures SOF 700RA 5 is a package of two MEDState Notation MSN procedures written for the control of intracranial self stimulation ICSS in threshold determination MED Associates Inc strongly encourages the creation of a backup copy of the procedures found on the ICSS CD Having copies of the original procedures is helpful in the case of computer failure and will provide a default copy of the original procedures in case changes to the programming code are necessary to implement different experimental protocol Reward mpc This procedure uses a Discrete Trial Method developed by Dr Conan Kornetsky Boston University School of Medicine in which the amplitude of both a free stimulus and a response contingent stimulus reward are the same It is used while testing a trained subject The program starts off with a free stimulation If the animal likes the stimulation and responds then it receives the same stimu
29. y 4 pWidth2 5 PAmp2 6 Freq 7 Dur 8 PAmpS2 9 List Data Variables Here NV A Data Variables Used as Listed NV A 3 of Reversals NV A 11 Total Cluster Responses NV A 12 Total ITI Responses NV A 13 Total Responses NV A 14 Total Free Stimuli NV C Master Data Array All data transferred to this array for Printing List Working Variables Here NV A Counter Variables Used as Listed A 0 of Non Responses in Descending Column or Responses in N Ascending Column in a 5 Trial Block If A 0 3 Increment A 2 NV A 1 of Reward Stimulations in a 5 Trial Block NV A 2 of Consecutive 5 Trial Blocks in which Criteria is Met N If A 2 2 Procedure Changes from Ascending to Descending Trial N Block and Vice Versa NV A 4 Ascending Descending Trial Block Tag N A 4 0 if Descending N A 4 1 if Ascending N Program Starts with a Descending Trial Block NV A 5 Trial Counter If A 5 5 Record Data and set up for new Series NV A 6 ITI Interval A 7 Cluster Responses N 1st amp all Subsequent Responses for First 2 of ITI 8 ITI Responses NV A 9 Tag for Responses made During Free Stimulus 1 Response Made N 0 No Response Made A 10 Block Counter Each Block Contains 5 Trials NV A 16 Value of Stim Current for Data Printout Gets set to the value of P 3 at Start of Procedure and after data is transferred to the C Array NV A 17 Value of Ascending
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