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Operating Manual Axiovert 200 / Axiovert 200 M Inverted Microscopes

1. 4 4 4 2 1 Performing CHECKS 254 09 sinere oiii Ra eee ese dre ER odisea 4 4 4 2 2 Changing the fuses on the TIC OSCODe ua cid etc an m m ERI BUE en 4 4 4 2 3 Changing the fuses on the 100 dc power 4 5 4 3 dts ate ae A MD pet a 4 6 List of illustrations Fig 4 1 Fig 4 2 CHANGING th Fuss ea e tea Y Vv e ecu ps en La de an 4 4 Changing the fuses on the power supply euer ee 4 5 B 40 080 e 03 01 4 1 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Carl Zeiss Contents List of illustrations Axiovert 200 4 2 40 080 e 03 01 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axiovert 200 Care Carl Zeiss 4 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE 4 1 Care Care of the instruments is limited to the following operations Cover the instrument with the dust cover after every use e Do not install instrument in a damp room Cover open tubes with dust protection caps e Remove dust and dirt from visible optical surfaces using a brush airblower Q tips optics cleaning paper or a cotton cloth e Remove water soluble dirt coffee Coke etc after breathing on it and wiping it off with a dust free cotton cloth or a moistened cloth A mild cleaning agent can also be added to the water e Remove stubborn oily or greasy dirt immersion oils
2. 3 8 B40 080e 03 01 Axiovert 200 OPERATION Binocular component of the tubes 3 1 14 The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another 3 5 A and 3 5 B Two vertical adjustments through rotation of the binocular component around 180 Eyepiece setting ring of eyepiece 3 1 16 and 15 Both eyepiece models permit compensation of the user s ametropia and also accept eyepiece reticles see chapter 2 LCD display 3 1 19 Two line display with 16 characters each attached to the carrier for transmitted light illumination condenser Display of objective position 3 6 1 magnification 3 6 2 and contrasting technique 3 6 3 in the upper line FL display 3 6 4 on the right of the top line if the fluorescence shutter is opened Display of lamp brightness in a line scale 3 6 6 and display of the relevant voltage 3 6 5 in the lower line If the color temperature 10 5 V has been set the line scale displays the value 3200 K One interval corresponds to 0 4 V If the halogen illuminator is switched off HAL off is displayed The LCD background illumination can be switched on or off as required Activation deactivation is performed by pressing the FL key for more than 2 seconds The LCD display is also used for the configuration of the microscope Set mode 40 080 03 01 Axiovert 200 Carl Zeiss
3. Fig 3 5 Setting of eyepiece distance on the binocular tube lt 1 2 3 4 S 6 P h 2 FL ul un 1111 x 6 5 Fig 3 6 LCD display 3 9 OPERATION Carl Zeiss Axiovert 200 Axiovert 200 Fig 3 7 LD condenser 0 55 6 position H Ph1 Ph2 Ph3 Var1 2 Fig 3 8 LD condenser 0 35 6 position H PhO Ph1 Ph2 DIC DIC Condensers 3 1 23 Depending on their type condensers 3 7 1 are equipped as follows 5 or 6 position turret for brightfield H phase contrast PhO Ph1 Ph2 with centerable stops interference contrast DIC Varel contrast Var1 Var2 Aperture diaphragm iris stop Use knurled knob 3 7 2 to open and close the aperture diaphragm Turn the turret wheel 3 7 4 to swing the brightfield insert or the contrast stops into the beam path The brief description of the set turret position e g H is displayed to the user at the front Condensers for Varel contrast are equipped with a knurled knob 3 7 3 which allows the position of the Varel stop to be changed when the Varel turret position has been set The lever 3 7 5 permits switching between Varl and Var2 The condensers 0 35 and 0 55 for phase contrast require the use of one SW 1 5 Allen screw driver each plugged in at 3 8 1 to center the phase stops Ks The condenser turrets feature the so called automatic diaphragm mechanism i e the aperture diaphr
4. 3 39 Pupil images VAREL a erue 3 39 Components for epi fluorescence on the Axiovert 200 3 41 Connection of a SLR Cameras ies oh edita tenes ead BIO Ree dence edo dieci eris fuses 3 45 VIG SO attache lE s can een uide ead 3 46 B40 080e 03 01 OPERATION Axiovert 200 Carl Zeiss 3 OPERATION The Axiovert 200 microscopes are offered in a manual version and in a version with motorized functions and components In the Operation chapter the manual setting and operation functions are explained first and the motorized coded functions are then explained separately where required However many operation functions are entirely identical in both instrument versions This manual will not deal with the possibilities of operating the motorized Axiovert 200 M together with a connected PC Equipment of the stands with an LCD display is possible as an option The LCD display can also be integrated subsequently but this must be performed by Zeiss service staff The Axiovert 200 microscopes have been designed for use with incubators and micromanipulators For information about connection possibilities and operation of these units please see the relevant separate manuals 40 080 03 01 3 3 OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3 1 Axiovert 200 manual 3 1 1 Operation and function controls on the Axiovert 200 ma
5. gt Gas discharge lamps e g HBO 100 emit ultraviolet radiation which can cause burns on the eyes and skin Therefore never look directly into the light of these lamps and avoid direct unprotected incidence of their light on your skin When using the microscope always use the protective devices belonging to the instrument e g special attenuation filters When hot gas discharge lamps are under high internal pressure and must therefore be changed when cooled down by using protective gloves and goggles for detailed information please see the operating instructions B 40 065 e When fluorescence filters are used the filter protecting from heat emitted by the microscope illuminator must not be removed since fluorescence filters are sensitive to heat and their function might therefore be impaired Placing objects against or covering ventilation slats can lead to a build up of heat which will damage the instrument and in extreme cases cause a fire Always keep the ventilation slats clear and make sure that no objects enter the instrument through the ventilation slats Avoid touching the hot lamp housings Always pull the power plug before changing the lamps and allow the instrument to cool down for approx 15 mins Dust and dirt can impair the performance of the instrument Therefore the instrument must be protected against these influences as far as possible and covered with the dust cover if it is not used for longer periods of
6. 2 20 2 12 2 Coarse alignment of halogen illuminator en an 2 21 2 12 3 Attachment of halogen 2 21 2 12 4 Fine alignment of halogen ale 2 22 0 4 B40 080e 03 01 INTRODUCTION Axiovert 200 Contents 2 13 Illuminator N HBO nn 2 13 1 Attachment change of the HBO 103 W 2 mercury pressure short arc lamp 2 13 2 Coarse alignment of illuminator N 103 2 13 3 Attachment of illuminator N HBO 103 2 2 13 4 Fine adjustment of illuminator N HBO 103 using the adjusting aid 2 14 Attachment or Aqua Stop en CHAPTER 3 OPERATION wisjsccisscersiscesaacnsivaseravaccencasiev ussws KG CR u TER E S EENE YA ERE 3 1 Axiovert 200 manual Hk is 3 1 1 Operation and function controls on the Axiovert 200 manual 3 1 2 Switching on and basic settings on the Axiovert 200 manual 3 2 Axiovert 200 M 2 eia era Peas 3 2 1 Operation and function controls on the Axiovert 200 motorized 3 2 2 Switching on and basic settings on the Axiovert 200 M motorized 3 3 Illumination and contrasting techniques sseen 3 3 1 Setting of transmitted light brightfield for K HLER illumination 3 3 2 Setting of transmitted light phase contrast 3 3 3 Setting of differential interference
7. F3 IJ ONO Ui 4 GJ NJ gt UJ hJ F2 XO CO O Installation Of ANICIOSCO PE rer ee Deed 2 5 Attachment of Binocular dee diede 2 6 Inserting the ee Eee 2 6 Inserting the eyepiece reticle uccisi inneren ee 2 7 Attachment of carrier for transmitted light illumination 100 2 8 Attachment of carrier for transmitted light illumination 30 2 8 Screw in objectives cio nee operiert ui te Ro wed du tec ds 2 9 Attachment of mechanical stage 130X85 2 10 Insertion of mounting frame K re een 2 10 Attachment of specimen stage 250 230 2 11 Attachment of object guide and mounting 2 11 Attachment of heating stage a euere 2 12 Attachment of condenser coe ed 2 13 Attachment of condenser rte ei uie 2 13 Changing the DIC DEISITn sess cte ei ee nee eed ttnn Ren 2 14 Insertion of reflector EUtTeL ecce eode ee nee 2 15 Insertion of reflector Modules eo ete re uino cte eet E toD E er Pe etude 2 15 Changing the filter set in the FL reflector module
8. e Remove illuminator from the microscope stand by loosening the clamping screw 2 35 2 Switch on the entirely connected illuminator N HBO 103 also see page 2 26 via the ebq 100 dc power supply 2 24 2 and allow it to heat to operating temperature Make sure not to look into the light exit opening of the illuminator e Direct light beam against a projection area wall approx 3 m away Use knurled knob 2 32 6 to focus on the brighter focal spot via collector adjustment Use adjusting screw 2 32 3 and SW 3 ball headed screwdriver to locate and focus on the slightly darker reflected focal spot Use adjusting screws 2 32 4 and 5 and SW 3 ball headed screwdriver to set the height and side of the brighter focal spot in accordance with the focal spot image 2 34 Fig 2 32 Coarse alignment of mercury vapor short arc lamp Fig 2 33 Focal spots of N 103 before coarse Fig 2 34 Focal spots of N HBO 103 after adjustment coarse adjustment 40 080 03 01 2 25 START UP Carl Zeiss Illuminator N HBO 103 Axiovert 200 If required the above settings for coarse adjustment of the N 103 illuminator can be performed repeatedly During coarse adjustment of the focal spots adjusting screws 2 32 1 and 2 marked red must not be changed since they influence the reflector setting in the HBO 103 illuminator 2 13 3 Attachment of illuminator N HBO 103 e Remove cover from reflected light tu
9. 000000 1005 816 50 vis 50 Sideport 60 left 0 vis 100 3 switching positions rc 000000 1005 817 50 vis 50 R Sideport 60 right 0 vis 100 R 3 switching positions 000000 1005 818 0 vis 100 L Sideport 60 left and right 3 switching positions 20 vis 80 R 5 Fo E 000000 1069 228 10096 Frontport Beam path switching 222 L 000000 1069 229 100 Frontport Beam path switching mot 9i A i Ps A A Za L 3 44 B40 080e 03 01 OPERATION Axiovert 200 Documentation Carl Zeiss Order No Viewed with Intermediate Direction of Viewing direction Description Switch position image Monitor motion of object schematic 000000 1005 824 100 doc 4 P Camera adapters working without intermediate imaging do not change the orientation of the image This also applies to the Frontport with adapters V200 T2 2 5x for SLR used on Frontport 000000 1005 869 and video adapter V200 C 2 3 0 63x used on Frontport 000000 1071 171 The use of Optovars 000000 1005 and 000000 1005 856 too does not change image orientation The above description also applies to any other specimen 3 4 2 Photomicrography with SLR camera The Axiovert 200 manual and Axiovert 200 M microscopes permit the beam path to be switched to 10096 vis visual observation through eyepieces and an open position
10. 2 16 Changing the Beam SDlEEQE ee ete to a ttp ee 2 16 Changing the se eed pev da dr ad et Qui do e dul diac 2 17 Axiovert 200 M real use 2 18 Power supply ebq 100 dc rear Zn Bea et 2 18 Axiovert 200 M Keane Hana ehe aided 2 19 Power supply 100 dc front 5 aot ee ek a 2 19 Changing the halogen a dad at Feux Vetus maet 2 20 Alignment of halogen IWM milnator s seines 2 21 Attachment of halogen dMidmliatOE aov stades ee ei 2 21 Changing ENC GIMUSION disk arena een 2 22 Power supply 100 dC e rer tne an e ehe 2 23 Removal or NBO 103 MOUSING aco aan 2 24 Changing the HBO 103 W 2 mercury pressure short arc 2 24 Coarse alignment of mercury vapor short arc 2 25 Focal spots of N 103 before coarse 2 25 Focal spots of N 103 after coarse 2 25 Attachment of illuminator N HBO TOS det a age e 2 26 Powersupply LOU IC een 2 26 Adjusting aid and illuminator N HBO 103 33 2 27 Attachment of AUG Stop ner RR 2 28 40 080 03 01 2 3 START UP Carl Zeiss List of illustrations Axiovert 200 2 4 B40 080e 03 01 START UP Axiovert 200 Unpacking and
11. Connection to the stand is by Amm banana plugs The connectors on the tubes are provided with M4 internal thread 40 080 03 01 Fig 2 23 Axiovert 200 M rear Fig 2 24 Power supply 100 dc front 2 19 START UP Carl Zeiss HAL 100 W halogen illuminator Axiovert 200 2 12 HAL 100 halogen illuminator 2 12 1 Change attachment of HAL 100 halogen lamp e Switch off the microscope remove connector 2 27 4 from the 12 V 100 W socket 2 27 5 and allow the illuminator to cool down for approx 15 minutes Loosen screw 2 25 5 until the lamp housing can be removed in an upward direction e Press both spring levers 2 25 3 downwards and pull out the old halogen lamp in an upward direction e Press both spring levers downwards insert new lamp 2 25 2 with protection cap 2 25 1 in lamp base 2 25 4 let go the spring lever and pull off the protection cap e Press spring lever briefly downwards again to center the lamp e Attach lamp housing again and tighten Fig 2 25 Changing the halogen lamp clamping screw 2 25 5 Ks After the lamp change the halogen illuminator must be realigned 2 20 B 40 080 e 03 01 START UP Axiovert 200 HAL 100 W halogen illuminator Carl Zeiss 2 12 2 Coarse alignment of halogen illuminator e Loosen the clamping screw 2 27 2 and remove the halogen illuminator from the carrier for tra
12. reed closed rooms er an T A ttam dera E SE E Protectiom EU DO a IP 20 Electrical Sal Cy use cts tte ae te in compliance with DIN EN 61010 1010 1 including CSA and UL directives Excess voltage categOty en a A ae Il Radio interference 5 5510 accordance with EN 55011 Class B Voltage ranges Integrated 12 V 100 W power unit stabilized 100 to 240 10 1 14 B40 080e 03 01 INSTRUMENT DESCRIPTION Axiovert 200 Technical Data Carl Zeiss Line fregUENCY 50 to 60 Hz Power consumption with internal power units AXlovert 200 asus le 235 VA 00 Meu ou detto estu odis edat ie ies ate eas qud th 235 VA and external power units Power supply N 75 100 240 V KC ttr att eere M RR 130 VA Power supply N HBO 100 90 240 VAT Cetera bte pe ted re EH eoi d E Pon uto ete ren 265 VA Fuses in accordance with IEC 127 Axiovert 200 1005240 VAC oan ied sante a dct dra ie uev ues T 4 0 AH 250 V 5 x 20 mm Axiovert 200 M 100 240 V BE dete nias su edis T 4 0 AH 250 V 5 x 20 mm Light sources HBO 50 W AC mercury pressure short arc lamp Lamp voltage for lamp type L1 and L2 L
13. FL shutter open FL appears in the LCD display or closed pressed long gt 2 5 background illumination of the LCD display is switched on or off If the key is kept pressed when the microscope is switched on the Light M anager is deactivated The Light M anager is activated automatically when the microscope is switched on the next time 3 22 B40 080e 03 01 OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss 3 2 2 Switching on and basic settings on the Axiovert 200 M motorized e Switch on the microscope with the on off switch on the right side The indicator light in the switch lights up Initialization of the instrument follows After initialization the LCD display shows the positions of all the stand components cyclically The display remains visible until a key is pressed 3 2 2 1 Configuration Set mode The microscope must be configured after it has been switched on i e the equipment of the nosepiece and the reflector turret the Z focus factors and the parfocal offset of the objectives to each other must be set Setting is performed in the Set mode with the aid of the LCD display The microscope is already configured in the factory in accordance with the equipment ordered by the customer When the equipment in the nosepiece and the reflector turret is changed the configuration must be updated accordingly Activation of Set mode To activate the Set mode keep the Set LM key 3 12 1 on the instrument rear pressed
14. After any accident with liquids you are strongly advised to remove the microscope stage and dab off any liquid drops using a lintless cloth Particularly clean the front lenses of the objectives to be able to fully utilize the full performance of the objective Objective type Magnification Contrasting Silicone ring Cat No num aperture size A Plan A Plan 5x 0 12 pho A Plan 10x 0 20 phi varl A Plan 20x A Plan 40x A Plan 100x LD A Plan 20x 0 30 LD A Plan 20x 0 30 LD A Plan 32x 0 40 LD A Plan 32x 0 35 LD A Plan 40x 0 50 LD A Plan 40x 0 50 ph2 var2 000000 1018 589 000000 1020 863 441041 0000 000 441051 0000 000 441080 0000 000 000000 1006 591 000000 1006 592 000000 1006 593 000000 1006 594 000000 1006 595 000000 1006 596 NNNNNNWWNN FA H H HH LD Achroplan Achroplan 4x 0 10 440020 0000 000 Achroplan 10x 0 25 phl 440031 0000 000 LD Achroplan 20x 0 40 440844 0000 000 LD Achroplan 20x 0 40 ph2 440845 0000 000 LD Achroplan 40x 0 60 440864 0000 000 LD Achroplan 40x 0 60 440865 0000 000 LD Achroplan 63x 0 75 ph2 440861 0000 000 Fluar Fluar 10x 440135 0000 000 Fluar 20x 440145 0000 000 Fluar 40x 1 3 Iris 440257 0000 000 40 080 03 01 2 29 START UP Attachment of Aqua Stop Axiovert 200 Objectivetype Magnification Contrasting Silicone ring Cat No num aperture size Carl Zeiss Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan
15. LSM Key pressed briefly current position is shown in the LCD display for 4 s or until the next press of the key without LCD display the position is approached immediately key pressed again target position is displayed and approached The set beam path is displayed depending on the VIS Frontport Baseport setting i e the actual intensities at the ports are displayed in Key for the motorized switching of the VIS Frontport Baseport slider 3 13 14 Key to switch the beam path between Frontport FP Baseport BP and VIS visual observation through the binocular The display in the LCD display depends on the position of the Sideport turret The following is displayed Binocular Baseport Sideport Empty positions are not approached or displayed unless a different setting has been made in the AXIOSET software program FCT key 3 13 15 The AXIOSET software program permits any required CAN SUB components to be assigned to this key Unless the key has been assigned otherwise the instrument is brought to a basic position Home or Default position after activation Basic position Optovar 1x pos 1 HAL 23V SP 100 96 VIS pos 1 BP 100 VIS pos 2 condenser aperture fully opened condenser turret BF pos 1 reflector turret on transmission FL shutter closed Key Set LM 3 13 18 briefly pressed Light M anager values are saved pressed long gt 2 5 activation of Set mode 40 080 03 01 3
16. Use the projections 2 28 3 on the mount of the diffusion disk to hold the component e Attach HAL 100 W and tighten the clamping screw 2 28 Changing the diffusion disk e Remove any swung in filter from the beam path e Use objective lt 40x to focus on the specimen and search for an empty object spot e Remove eyepiece and use adjusting screws 2 26 4 and 5 to center the lamp coil and its reflected image in the pupil image e Use adjusting screws 2 26 3 to optimize the homogeneous illumination of the pupil image e Remove HAL 100 W after conclusion of the alignment e Screw diffusion disk into the carrier again manually e Attach HAL 100 W and swing in available filters again 2 22 40 080 e 03 01 START UP Axiovert 200 Illuminator N HBO 103 Carl Zeiss 2 13 Illuminator N HBO 103 2 13 1 Attachment change of the HBO 103 W 2 mercury pressure short arc lamp The illuminator N HBO 103 and the HBO 103 W 2 mercury pressure short arc lamp are supplied to the customer in separate packaging for safety reasons Therefore insertion of the HBO 103 W 2 into the lamp housing is the first step in the start up of the illuminator Before opening the lamp housing make sure that no connection to the electrical line is available AN The HBO 203 W 2 lamps may only be changed after they have cooled down Allow the N HBO 103 microscope illuminator to cool down for approx 15 minutes to avoid the risk of explosion
17. finger prints with a wad of cotton or a dust free cotton cloth dipped in the optics cleaning mixture L This cleaning mixture is produced of 90 Vol benzoline and 10 Vol isopropanol IPA The various components are also known under the following synonyms benzoline medical alcohol petroleum ether Isopropanol 2 propanol dimethyl carbinol 2 hydroxypropane Clean the optical surface by moving in circles starting in the middle Slight pressure should be exerted on the optics during cleaning When using the Axiovert 200 in humid climatic zones proceed as follows e Store the microscope in bright dry and well ventilated rooms with a humidity of less than 6596 store particularly sensitive components and accessories such as objectives and eyepieces in a dry closet e When the equipment is stored in closed cases for a longer period of time the growth of fungus can be avoided by including cloths soaked in fungicide in the cases 1 The risk of growth of fungus on opto mechanical instruments always exists in the following conditions relative humidity of more than 75 and temperatures between 15 and 35 for more than three days installation in dark rooms without air ventilation and dust deposits and fingerprints on optical surfaces 40 080 e 03 01 4 3 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Carl Zeiss Maintenance Axiovert 200 4 2 Maintenance 4 2 1 Performing checks The following checks should be
18. 000 Universal mounting frame M 471718 0000 000 Universal mounting frame M Duo 000000 1116 077 Object guide 130x85 mm right 000000 1005 833 Object guide 130x85 mm left 000000 1110 991 Specimen stage 250x230 mm 000000 1005 832 Mechanical stage 130x85 R L without mounting frames 000000 1005 834 Fig 1 1 1 6 Mounting frame M for microtiter plates 96 positions Microscope configurations and modules Microscope configurations and modules Axiovert 200 DIC prism 1 0 35 000000 1005 821 DIC prism 11 0 35 000000 1005 822 Gliding stage Z with stage inserts d 24 and 48mm 471722 0000 000 LD condenser 0 35 H DIC Ph 0 Ph 1 Ph 2 Var 1 000000 1005 844 LD condenser 0 35 H Ph 0 Ph 1 Ph 2 DIC DIC 000000 1005 845 e Achromatic condenser 0 8 DIC 000000 1087 444 DIC prism 8 v 000000 1087 445 or one object carrier PC 000000 1 116 088 Auxiliary microscope d 30 000000 1006 362 D Eyepiece eyecup 444801 0000 000 Eyepiece E PL 10x20 Br 444231 9901 000 Eyepiece E PL 10x20 Br foc 4444232 9902 000 Eyepiece W PL 10x23 Br foc 000000 1016 758 Mounting frames for mechanical stage Mounting frame K 451341 0000 000 Universal mounting frame K X 451353 0000 000 Universal mounting frame K 451352 0000 000 Universal mounting frame K Duo 000000 1116 078 Mounting frame K d 72mm 451344 0000 000 Stage stop d 28 mm 4513
19. 03 01 3 39 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3 3 5 Setting of fluorescence contrast in reflected light 3 3 5 1 General principle The epi fluorescence technique permits high contrast images of fluorescent substances in typical fluorescence colors In the epi fluorescence microscope the light generated by a high performance illuminator reaches the excitation filter via a heat reflecting filter The filtered short wave excitation emission is reflected from a dichroic beam splitter and is focused on the specimen via the objective The specimen absorbs the short wave emission and then emits long wave fluorescence STOKE s law which is now gathered by the objective and transmitted by the dichroic beam splitter Finally the beams pass a barrier filter which only allows the long wave emission from the specimen to be transmitted Excitation and barrier filters which are positioned in the FL reflector module together with the appropriate dichroic beam splitter must be perfectly matched 3 3 5 2 Configuration of the Axiovert 200 manual and Axiovert 200 M Recommended objectives brightfield objectives FL reflector module in the reflector turret N HBO 103 fluorescence illuminator or HBO 50 for reflected light illumination HAL 100 halogen illuminator for transmitted light illumination D Before the epi fluorescence technique is applied it is absolutely necessary to adjust the mercury vapor short arc lamp i
20. 1 Carl Zeiss A 2 ANNEX Overview Axiovert 200 B 40 080 e 03 01 Axiovert 200 ANNEX List of abbreviations Carl Zeiss List of abbreviations a AC A Plan Br CAN bus CSA DC D d DIC DIN doc EG EN EMV EWG foc HAL HBO HD ICS IEC ISO CD LD M mot N A Free working distance Alternating Current Achromatic objectives featuring improved image flatness ICS line suitable for eyeglass wearers Controller Area Network Communication Bus Canadian Standards Association Direct Current transmitted light cover slip thickness diameter Differential Interference Contrast German standards association documentation European Community European standards electromagnetic compatibility European Economic Community focusing brightfield halogen illuminator mercury vapor short arc lamp brightfield darkfield Infinity Color Corrected System International Electrotechnical Commission International Protection protection class International Organization for Standardization Liquid Crystal Display Long Distance motoriziert numerical aperture B 40 080 e 03 01 A 3 Carl Zeiss Ph PL SB SLR SW VIS XBO A 4 ANNES List of abbreviations phase contrast flatfield Sub Bus Single Lens Reflex wrench opening slow blow fuse type Television Underwriter Laboratories visual Xenon short arc lamp Axiovert 200 40 080 e 03 01 ANNEX Axiovert 200
21. 1005 848 from objective 4x Condenser 0 35 6 positions H DIC PhO Ph1 Ph2 DIC DIC 000000 1005 845 Condenser 0 55 5 positions H Ph1 Ph2 Ph3 Var1 2 000000 1005 846 Condenser 0 55 6 positions H Ph1 Ph2 Ph3 DIC DIC 000000 1005 847 Condenser adapter for condensers 0 8 and 1 4 000000 1005 849 Condenser 0 8 H D 0 66 0 8 Ph DIC plus factory aligned DIC prism III 0 8 445445 9901 000 445485 0000 000 Condenser 0 8 H DIC plus factory aligned DIC prism III 0 8 000000 1087 444 000000 1087 445 Condenser 1 4 6 positions H D Ph 2x DIC 2x 445453 0000 000 1 12 B40 080e 03 01 INSTRUMENT DESCRIPTION Axiovert 200 Specimen stages and mounting frames Binocular tubes Carl Zeiss 1 7 Specimen stages and mounting frames The Axiovert 200 microscopes can be equipped with the following specimen stages and mounting frames Description Cat No Specimen stage 250x230 mm with ceramic surface and 3 point support 000000 1005 832 Object guide 130x85 right can be attached on both sides accepts various mounting frames 000000 1005 833 Object guide 130x85 left can be attached on both sides accepts various mounting frames 000000 1110 991 Mechanical stage 130x85 R L 000000 1005 834 Mounting frame for object guide see price list Mounting frame for mechanical stage K see price list Gliding stage Z 471
22. 12 B40 080e 03 01 START UP Axiovert 200 Attachment of condensers Carl Zeiss 2 6 Attachment of condensers 2 6 1 Condensers for the Axiovert 200 e Insert condenser 2 13 1 into the condenser carrier on the carrier for transmitted light illumination with its dovetail pointing upwards Make sure that the orientation pin of the condenser is positioned at the front and exactly engages into the guiding groove of the condenser carrier e Fix condenser in position with clamping screw 2 13 2 For motorized condenser connect the cable to the SB connector on the rear of the stand Fig 2 13 Attachment of condenser 2 6 2 Condensers from the Axioplan 2 imaging Axioskop 2 product line The following condensers from the Axioplan 2 imaging Axioskop 2 line can be used Achromatic condenser 0 8 H D Ph DIC 445445 9901 000 Achromatic aplanatic condenser 14 H D Ph DIC 445453 0000 000 The inverted design of the Axiovert 200 requires reversed integration of the condensers from the Axioplan 2 imaging Axioskop 2 line i e the turret must point to the back so that the control elements are positioned at the rear and the labeling is upside down Achromatic condenser 0 8 H DIC 000000 1087 444 Fig 2 14 Attachment of condenser adapter 40 080 03 01 2 13 START UP Carl Zeiss Attachment of condensers Axiovert 200 e Insert condenser adapter 2 14 1 into the condenser carri
23. 2 19 Equipotential bonding terminals essen eqs Ruta uda UN ope CHO ice aa 2 19 HAL 100 halogen illuminator ea en ec 2 20 Change attachment of the HAL 100 halogen 2 20 Coarse alignment of halogen illuminator esen nnn 2 21 Attachment or halogen illuminator aa er 2 21 Fine alignment of halogen illuminator s essen 2 22 liumimator N HBOALUS ut ee d i tat ede eter aii 2 23 Attachment change of the 103 W 2 mercury pressure short arc lamp 2 23 Coarse alignment of illuminator N HBO 103 redd eee natn se 2 25 Attachment of illuminator N HBO 103 een 2 26 Fine adjustment of illuminator N 103 using the adjusting 2 27 Attachment of Aqua SEODm iiie lee e dels e Mee e lea 2 28 B40 080e 03 01 START UP Axiovert 200 List of illustrations Carl Zeiss List of illustrations Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig NO NO NO NO NO NO NO NO 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 U CU CU NJ NO NO NO NO NO NJ
24. 26 B 40 080 e 03 01 OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss 3 2 2 3 Light Manager The Light Manager is applied with the Axiovert 200 M storage of set values in the same way as with the manual version However the functionality of the Light Manager is considerably greater in the Axiovert 200 M on account of the coding motorization of the major microscope components The Light M anager is automatically activated each time the Axiovert 200 is switched on However there is the possibility of deactivating the Light M anager temporarily Temporary deactivation is achieved by keeping the key FL on off pressed after the instrument is switched on until a double beep is emitted The Light Manager is then deactivated until the microscope is switched on the next time LM off appears in the LCD display In transmitted light the following parameters are stored with each nosepiece position illumination intensity of the halogen illuminator condenser turret position i e one contrasting technique e g H Ph DIC per nosepiece position aperture stop opening The intensity value is stored for each Optovar turret position in combination with an objective i e the illumination intensity is always suitable after a change of the Optovar magnification The condenser turret position does not influence the illumination intensity In reflected light the position of the fluorescence shutter is also taken into consideration
25. 43 E Equipotential bonding terminal an een 2 19 eyepiece reticle aa EIKE s Gel Me Pad 2 7 dc 2 6 2 7 3 9 up 3 21 filter Can Gelert tac ER 3 11 ze De EEUU 3 13 c 3 13 3 22 PPL module unse Re RE 2 16 40 080 e 03 01 A 5 ANNEX Carl Zeiss List of key words Axiovert 200 F fl or scence contrast au idt eoi ee ee ine nr dude cR MEUS 3 40 fluorescence illuminator ss ee ana nis 3 42 PE 3 15 FOCUS BD oda 3 18 MI een 3 26 focusiNg ATIVE EE 3 6 3 17 FFONEDOTE MEER 3 11 H ek ee 3 17 ll minato r NI BOS TUS ee ee Re Ar kenne 2 23 NAGESOTIENLALIO N ocn cse re ee 3 43 ee 2 5 HS StOp Slider 3 13 K key ee ea Eigenen iii 3 6 KOHLER a an dics Apri 3 11 3 13 L LC ERI Den ee se ee 3 9 3 19 LEFT RIGHT te a ee N ee 3 21 Ehud 3 15 3 27 ECHO ea AI LA Er 2 18 LORD a as Mu UE E 3 18 luminous field diaphragm transmitted light usseeeersssssnnneennnnnn
26. Carl Zeiss Axiovert 200 Axiovert 200 On Off switch 3 1 1 position 0 instrument switched off position instrument switched on switch lights up Key HAL on off 3 1 10 Brief pressing switches the halogen illuminator on or off alternately Long pressing 1 s automatically sets the brightness to 3200K for color photography Toggle switch for brightness control of the halogen illuminator 3 1 11 Controls the brightness of the halogen illuminator in 2 steps step 1 slow brightness change switch pressed half down until first pressure point switch to the left brightness reduction switch to the right brightness increase step 2 fast brightness change switch pressed down until stop second pressure point switch to the left brightness reduction switch to the right brightness increase Variable voltage range between 0 and 12 V When the limit values are reached a warning beep is emitted At 10 5 V the halogen lamp provides light with the color temperature of 3200 K for color photography To make this setting easier for you the fast increase of the lamp voltage stops at this point and a beep is emitted To further increase the lamp voltage keep the toggle switch pressed for more than 1 sec or release it briefly and then press it again If the toggle switch is pressed down for more than 3 sec while the halogen illuminator is switched off the halogen illuminator will automatically switch on again Focusing drive
27. List of key words Carl Zeiss List of key words Page 3 32008 GE 3 6 3 17 A BC Allan ee ce cel Fn HC 3 13 ametropia enaren arar ee ee Teer 2 7 AN AS Z ET ee a eure 3 7 Maer ohn St oan edt par ees Sone ec REN So og 3 32 3 42 attachment of BUD oar che dca bean 2 6 B a ea er ee 3 12 basic re tr o d I to Poo 3 14 3 16 M DL M Sanches De ach een nenn 2 16 DINOCUIAE component nee 3 9 EU SS sss MEE 3 8 E 3 28 brightness control of halogen MIUMIN ALON use een 3 6 C s T 4 3 changing 4 4 4 5 color MMC E T RE 3 6 3 17 asinis ene au re ee en ONTA een 2 13 3 10 3 11 CONAN SCH ui M TL 3 20 ne 3 23 contrasting Tec near 3 28 DIC 2 14 DIC differential interference 5 3 3 35 IS en E N DEA 2 22 dot mentati A een ernennen 3
28. Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Apochromat Plan Apochromat Plan Apochromat 2 30 5x 0 15 5x 0 15 10x 0 30 10x 0 30 16x 0 50 Imm 16x 0 50 Imm 20x 0 50 20x 0 50 40x 0 75 40x 0 75 40x 1 30 Oil 40x 1 30 Oil 63x 0 95 Korr 63x 1 25 Oil Iris 100x 1 30 Oil 100x 1 30 Oil 63x 1 40 Oil 10x0 45 NA 4 GJ CJ NO DYN DN FA E HH HR pn mpm pm mn 440320 0000 000 440321 0000 000 440330 0000 000 440331 0000 000 440530 0000 000 440531 0000 000 000000 1004 072 000000 1004 989 440350 9902 000 440351 9902 000 000000 1022 818 000000 1022 819 440364 0000 000 440466 0000 000 000000 1018 595 000000 1031 171 440760 0000 000 440639 0000 000 B40 080e 03 01 OPERATION Axiovert 200 Contents Carl Zeiss OPERATION Contents w w w N e 3 2 1 3 2 2 3 3 33L 3 3 2 3 3 3 3 3 4 3 3 5 3 4 3 4 1 3 4 2 3 4 3 3 3 AXIOVEerE 200 manual A eaan delet rui 3 4 Operation and function controls on the Axiovert 200 3 4 Switching on and basic settings on the Axiovert 200 3 14 Axiovert 200 M motorized cx e
29. Right 20 upper line of the LCD display and Binocular 80 lower line FOCUS Load Load Position appears in the lower line display blinks every second ZERO display of the current focus position in mm e g Zdis 0 0pm motorized condenser is connected the currently set condenser turret position appears in the lower line e g Cond DICIII If the aperture is changed by pressing the key on the condenser the value of the stop opening is displayed e g Cond NA 0 55 When a phase stop position is set the aperture diaphragm is always entirely open Display function during the instrument configuration SET mode 40 080 03 01 3 19 OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 Motorized condensers 3 13 16 Motorized condenser equipped with 6 position turret for brightfield H phase contrast Ph1 Ph2 Ph3 with centerable stops interference contrast DIC DIC II f a motorized condenser is recognized by the Sub bus of the stand the current condenser turret position automatically appears in the lower line of the LCD display The condenser display is always available Motorized adjustment of the turret by pressing key Rev V forward and backward 3 15 1 on the right side of the condenser Motorized adjustment of the aperture diaphragm by pressing key A open and close 3 15 2 on the left side of the condenser If a phase stop is contained in the beam path the ape
30. Screw IN OBJECTIVES nodos oie en 25 Attachment of microscope Stages mu ana Oe 2 5 1 Attachment of mechanical stage 130x85 R L and mounting frame for mechanical stage K 225 ded tee cette beaut tbe erae teo rai ot edad rant 2 5 2 Attachment of scanning stage areas 2 5 3 Attachment of specimen stage 250x230 object guide and mounting frame for object guide M ae ee een 2 5 4 Attachment of heating Stage nn reine 2 5 5 Attachment of gliding Stage Z oed eroe ri ean ea gebe Renee Exo Et Cer tees 2 6 Attachment of condensers 2 des eode dedi c duo ed 2 6 1 Condensers for the Axiovert 200 2 6 2 Condensers from the Axioplan 2 imaging Axioskop 2 product line 2 6 3 Changing the DIC prism in the condenser 2 1 Reflector tU EE ee rer enable 2 7 1 Attachment of reflector Lufret 4 2er 2 7 2 Equipment of reflector 2 7 3 Changing the filter set the FL reflector module 2 7 4 Changing the beam splitter in the FL reflector 40 080 03 01 Carl Zeiss 2 1 Carl Zeiss 2 2 START UP Contents Axiovert 200 Connection tothe lernen ee inne 2 18 Interfaces of Ihe Axiovert 200 M sense en 2 19 Switch microscope and 100 dc power supply on and
31. The position of the reflector turret switches the halogen illuminator and the fluorescence shutter If a fluorescence filter set is swung in on the reflector turret the halogen illuminator is switched off and the fluorescence shutter is opened If no fluorescence filter set brightfield is swung in or configured the halogen illuminator is switched on and the fluorescence shutter is closed If the halogen illuminator shall be used parallel to the reflected light illuminator it can be switched on via the Hal on off key the illumination intensity can be matched and this setting can also be stored by briefly pressing the Set LM key instrument rear Standard settings are performed and stored in the factory for all components integrated into the Light M anager function The settings of the Light Manager can be manually adapted to the available conditions any time If manually changed settings shall be stored permanently this is possible by briefly pressing the SET LM key A brief beep after release of the key confirms that storage has been performed 40 080 03 01 3 27 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3 3 Illumination and contrasting techniques 3 3 1 Setting of transmitted light brightfield for KOHLER illumination 3 3 1 1 General principle Transmitted light brightfield microscopy is the most usual of all the optical techniques since it allows the easy and fast viewing of high contrast or
32. carrier 2 8 3 for transmitted light illumination can be tilted backwards Place mechanical stage 2 8 2 on the three contact points 2 8 4 of the stand and fix it in position using three hexagonal screws 2 8 1 two at the front one at the rear Three countersunk holes each on the front and rear of the mechanical stage 130x85 R L permit attachment with the drive knobs being positioned on the right or on the left Then insert the mounting frame K 2 9 1 into the mechanical stage For this purpose position the red dot 2 9 2 of the corner of the mounting frame on the red dot of the mechanical stage 2 9 3 and press the mounting frame diagonally against the springs and downwards into the recess Make sure that the mounting frame is seated correctly 2 5 2 Attachment of scanning stage The scanning stage is attached in the same way as the mechanical stage However the three spacers 4 mm enclosed with the stand must be inserted before attachment of the scanning stage The cable to the separate motor control unit must then be connected B40 080e 03 01 Axiovert 200 N 2 5 3 START UP Because of the large travel range of the scanning stage it may happen that the stage frame collides with the objectives at the end of the stage travel Attachment of specimen stage 250x230 object guide and mount ing frame for object guide M The specimen stage is attached to the contact points of the stand
33. for approx 2 seconds until a double beep is emitted The objective name default or the reflector name in the LCD display starts blinking D If no adjustment is performed for more than 60 seconds the Set mode is exited automatically 40 080 03 01 3 23 OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 Setting of nosepiece equipment Pressone ofthetwo OBJECTIVE keys Change to nosepiece programming is made and SET Objective is displayed in the upper line of the LCD display The set nosepiece position the magnification and the contrasting technique of the objective are displayed in the lower LCD line e Activation of the toggle switch on the front of the stand permits a list of objectives to be scrolled through upwards or downwards e f the correct objective for the relevant nosepiece position appears in the LCD display the next nosepiece position can be swung in by pressing one of the two OBJECTIVE keys and the relevant objective can be assigned the procedure for all nosepiece positions Then change to the next programming item or exit the Set mode Setting of reflector turret equipment Press one of the two REFLECTOR keys Change to reflector turret programming is made and SET Reflector is displayed in the upper line of the LCD display The set reflector turret position and the selected filter set are shown in the lower LCD line e Activation of the toggle switch on the front of the stand permits
34. insert retainer ring again and screw it tight using the mounting device e Use plastic cover to close the assembly opening again e Make sure that the knurled ring of the turret disk is labelled correctly e Turn the condenser around and insert it in the carrier for transmitted light illumination 2 14 40 080 e 03 01 START UP Axiovert 200 Reflector turret Carl Zeiss 2 7 Reflector turret 2 7 1 Attachment of reflector turret The manual reflector turret can be pushed into the stand either from the right or from the left depending on which side is accessible The motorized reflector turret can only be inserted from the right with the instrument being switched off e Insert equipped reflector turret 2 16 1 in the mount provided below the nosepiece Ensure the correct stop position e Tighten fixation screw 2 16 2 for the reflector Fig 2 16 Insertion of reflector turret turret on the right side is When changing the reflector turret close the FL shutter to avoid stray light e The protective lens available as an option can be inserted into the opening of the reflector turret 2 16 3 2 7 2 Equipment of reflector turret The reflector turret is usually entirely equipped when delivered from the factory However equipment with the filter sets contained in the reflector modules can also be easily performed by the customers themselves e Loosen fixation screw for reflector turret 2 16 2 on the right
35. installation of microscope Carl Zeiss 2 START UP On account of the complexity of the equipment and to ensure proper functioning installation and initial start up of the Axiovert 200 or Axiovert 200 M at your site will be performed entirely by the responsible Carl Zeiss agency The following major services will be performed Installation of the microscope assembly and alignment of all components if these are not already factory aligned Connection of cables and line connection nstrument training Should you want to install the instrument yourself or move it to another position proceed as described below 2 1 Unpacking and installation of the microscope The basic instrument is supplied in a commercially available polyethylene case in cardboard packaging This package contains the following stand binocular tube objectives eyepieces condenser halogen illuminator fluorescence illuminator and various small components such as filter and diaphragm slider DIC slider dust cover tools etc Further optional accessories are supplied in a separate case e Remove all components from the packaging and use the delivery note to check for completeness e Place stand 2 1 1 on a low vibration flat worktable Properly dispose of original packaging or keep it for storage or return of the instrument to the manufacturer e Unscrew handle 2 1 2 using the SW 4 Allen key Fig 2 1 Installation of micr
36. is positioned on the rear of the power supply It is combined with the instrument socket and contains two T 3 15A fuses If fuses blow the cause must be found out first and any technical defect must be efficiently eliminated Always pull the power plug before changing the fuses e Disconnect the instrument from the line e Pull fuse holder 4 2 1 out of the fuse compartment 4 2 2 in forward direction e Replace defective fuse e Then push fuse holder back into the fuse compartment until click stop e Plug in power cords 40 080 e 03 01 Fig 4 2 Changing the fuses on the power supply 4 5 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Carl Zeiss Service Axiovert 200 4 3 Service All repairs of optical or electronic components inside the instrument or any repair of the power supply may only be performed by service staff or specially authorized personnel If service is required please get in touch with your local agency or with Carl Zeiss Light Microscopy P O B 4041 37030 Gottingen GERM ANY Phone 1 449 551 5060 660 Telefax 1 449 551 5060 464 Internet www zeiss de micro E M ail micro zeiss de 4 6 B 40 080 e 03 01 ANNEX Axiovert 200 Overview Carl Zeiss Annex Eist of abbreviations Zee nee is cada teed A A 3 RISE OT Key NOS en LU ratu A 5 Certification in accordance with DIN ISO 9001 EN 46001 EC Declaration of Conformity B 40 080 03 01 A
37. modules or single parts This also applies to all service or repair work which is not carried out by authorized service personnel Furthermore this forfeits all the claims against warranty The power plug must be inserted in a socket featuring a grounding earth contact The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire If it is established that the protection measures are no longer effective the instrument must be switched off and safeguarded against inadvertent operation For repair of the instrument contact the Carl Zeiss microscope service in Germany see page 4 6 or your local Carl Zeiss agency The Axiovert 200 microscopes are not equipped with any special devices for protection from substances which are corrosive toxic radioactive or otherwise hazardous to health All the legal regulations for accident prevention particularly those in the respective countries must be observed when handling such substances Before switching on the power unit for the HBO 50 100 check whether it is suitable for the line voltage present Always disconnect the instrument from the line before opening the instrument and before changing the fuses M ake sure to use only fuses of the rated power required The use of makeshift fuses and the short circuiting of the fuse holders are not permitted 40 080 e 03 01 INTRODUCTION Axiovert 200 Notes on instrument safety Carl Zeiss
38. motorized 3 2 1 Operation and function controls on the Axiovert 200 motorized B Fig 3 13 Operation and function controls on the Axiovert 200 M motorized 3 16 B 40 080 03 01 Axi OPERATION overt 200 Axiovert 200 M Key to Fig 3 13 On Off switch Nosepiece motorized Reflector turret motorized OBJECTIVE keys forward and backward REFLECTOR keys forward and backward Focusing drive coarse fine motor supported ZERO key FOCUS keys Work Load HAL on off key FL key on off fluorescence shutter Toggle switch for illumination intensity and programming 1x OPTOVAR key maximum of 3 positions Left Right key for Sideport switching Key for switching between visual observation Frontport Baseport FCT key programmable function key Condenser motorized LCD display Key Set LM RS 232 connector CAN SB connector CAN Bus Sub bus On Off switch 3 13 1 position 0 instrument switched off position instrument switched on switch lights up HAL key on off 3 13 9 Brief pressing switches the halogen illuminator on or off alternately Carl Zeiss When the unit is switched on again the voltage present at the time of switching off is automatically set again When the unit is switched on the lower line of the LCD display shows the voltage in a scale and the lamp voltage value nomin
39. on the setting wheel 3 16 4 of the Optovar turret ensure the correct stop position Open the luminous field diaphragm completely by pulling the lever 3 16 16 on the carrier for transmitted light illumination to the front until stop Open the aperture diaphragm completely by turning the setting wheel 3 16 20 on the condenser to the front until stop Turn the setting ring 3 16 19 to move the condenser turret in position H for brightfield if not available to position DIC Turn the setting ring to move the reflector turret 3 16 5 if available in a position without filter combination and ensure the correct stop position If required remove analyzer slider from the compartment 3 16 3 or switch to free light path ensure the correct stop position Turn setting wheel for Sideport right Sideport left vis 3 16 22 to position 100 96 vis visual Turn setting knob for Frontport Baseport vis 3 16 23 to position 100 96 vis Set beam splitting ratio to 100 96 vis 3 16 10 on the binocular photo tube Switch off the Bertrand lens if available M ove combined rotary slider knob 3 16 9 to position 100 96 vis 40 080 03 01 3 29 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 A e 2 A J 23 24 13 9a x 12 M 11 10 9 1 On Off switch 10 Rotary or slider knob for Bertrand 18 Centering screw for condenser 2 Nosepiece lens and man
40. performed every six months General e Check line cables and plugs for defects e f any damage is visible switch off the instrument Have defects repaired by Zeiss service staff e Make sure that the maximum operation time of the halogen and HBO XBO lamps is not exceeded once a week Illumination e Check the correct setting of the halogen and XBO lamps e Check contacts of the electrical connection points of the illuminators Optics e Visual checking of cleanliness of objectives and eyepieces 4 2 2 Changing the fuses on the microscope The fuses for the instrument input voltage are positioned on the rear of the microscope stand The fuse compartment which contains two T4 0 A H 250 V 5x20 mm fuses is combined with the instrument socket If fuses blow the cause must be found out first and any technical defect must be efficiently eliminated Always pull the power plug before changing the fuses e Disconnect the instrument from the line Pull out fuse compartment 4 1 1 in forward direction Fig 4 1 Changing the fuses e Remove fuses from the holder and insert new fuses e Insert fuse holder back in the fuse compartment 4 1 2 until stop Plug in power cords 4 4 40 080 e 03 01 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axiovert 200 Maintenance Carl Zeiss 4 2 3 Changing the fuses on the ebq 100 dc power supply The fuse holder for the fuses Fl and F2
41. side e Pull reflector turret out of the stand and place it on a suitable support sturdy worktable e Carefully press both holding catches 2 17 2 on the right and left of the plastic hood away from Fig 2 17 the pins in outward direction and remove plastic hood in upward direction e Insert reflector modules 2 17 1 in the relevant reflector position according to the filter combination see engraved number starting with position 1 emission filter lies at the bottom First insert the reflector module into the two lower spring clamps at an angle from above using the holding elements on the right and left and then press the module against the upper spring clamps from the front until the module click stops into position Insertion of reflector modules 40 080 03 01 2 15 START UP Carl Zeiss Reflector turret Axiovert 200 To remove reflector module no longer required first pull it out of the upper spring clamps and then from the lower ones When equipment has been finished attach the plastic hood again and press it downwards until the holding catches on the right and left engage in the lower part of the reflector turret 2 7 3 Changing the filter set in the FL reflector module The filter sets for the FL reflector module can be compiled and assembled by the customer himself as required Suitable filter sets or completely assembled FL reflector modules can be ordered from Carl Zeiss e Re
42. stages incubators and micromanipulators M ajor instrument features ICS optics for image creation high thermal and mechanical stability high flexibility in documentation improved ergonomic design LCD display of instrument parameters 23 mm field of view Light Manager modular design for optimum adaptation to the relevant application 6 position nosepiece coded 5 position reflector turret manual can be changed on both sides motorized can be changed from the right 5 position or 6 position condenser turret 3 position Optovar turret changeable aperture diaphragm and luminous field diaphragm sliders in reflected light fluorescence shutter illuminators HAL 100 W HBO 50 N HBO 103 N XBO 75 Axiovert 200 M all the major microscope functions are motorized 40 080 03 01 1 5 INSTRUM ENT DESCRIPTION Carl Zeiss 13 Mounting frames for specimen stage with object guide Mounting frame M for specimen slider 76 x 26 471719 0000 000 Mounting frame M for microtest plates 471747 0000 000 Mounting frame M for Petri dishes dia 36 mm 471742 0000 000 Mounting frame M for Petri dishes dia 54 mm 471743 0000 000 Mounting frame M for Petri dishes dia 65 mm 471758 0000 000 Mounting frame M for Petri dishes dia 88 mm 471759 0000 000 471746 0000 000 Mounting frame M for multitest dishes 471744 0000 000 Universal mounting frame M X 471717 0000
43. stained specimens e g blood smears In addition to so called direct beam bundles indirect bundles which are diffracted and scattered at the specimen details are of major importance for an image as true to the object as possible The greater the portion of the indirect bundles aperture the more the microscope image will be true to the object according to ABBE To make use of the entire optical performance of the microscope and the objective in particular the condenser the luminous field diaphragm and the aperture diaphragm should be set in accordance with the rules of KOHLER illumination These basic rules of microscope setting are described in detail in the following section 3 3 1 3 Transmitted light brightfield setting according to KOHLER on the Axiovert 200 3 3 1 2 Transmitted light brightfield configuration of the Axiovert 200 manual Each manual Axiovert 200 microscope can be configured to permit the transmitted light brightfield technique 3 28 B40 080e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss 3 3 1 3 Transmitted light brightfield setting according to KOHLER on the Axiovert 200 manual Put the Axiovert 200 manual in operation as described in chapter 2 Switch on the Axiovert 200 manual Select the objective with the lowest magnification e g 10x objective with the yellow ring on the nosepiece 3 16 2 and ensure the correct stop position Set factor 1x
44. time Always check whether the instrument is switched off before you cover it The instruments may only be operated by trained personnel who must be aware of the possible danger involved with microscopy and the relevant application The Axiovert 200 microscopes are opto mechanical precision instruments which can be impaired in their performance or damaged when handled improperly 40 080 03 01 0 9 INTRODUCTION Carl Zeiss Notes on warranty Axiovert 200 Notes on warranty The manufacturer guarantees that the instrument has no material and production defects when delivered You must inform us of any defects immediately and we must do anything to minimize the damage If the manufacturer is informed of such a defect he is obliged to remove it it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect No guarantee is provided for defects caused by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any other meddling with the instrument particularly the removal or replacement of instrument components or the use of accessories from other manufacturers This forfeits all the claims against warranty With the exception of the work specified in this manual no maintenance or repair of the microscopes may be performed Repairs may only be performed by Carl Zeiss service staff or spe
45. two centering screws to center the luminous field diaphragm in the edge of the field of view Either open the luminous field diaphragm until it just disappears behind the edge of the field of view or if there is a risk of specimen bleaching reduce it until it is visible in the field of view Finally refocus on the specimen and optimize the collector position of the fluorescence illuminator For this purpose use the knurled knob on the fluorescence illuminator to set the collector in such a way that illumination of the field of view provided by the short wave excitation reflector module is as homogeneous as possible Correction of the collector position is not required when modules with long wave excitation are used 3 42 40 080 e 03 01 OPERATION Axiovert 200 Documentation Carl Zeiss 3 4 Documentation Depending on the instrument configuration the Axiovert 200 manual and Axiovert 200 M microscopes can be equipped with up to five documentation ports Frontport forthe connection of aSLR camera a video or digital camera e g AxioCam from Zeiss via a special video or camera adapter Sideport right or left for the connection of documentation equipment via the 60 mm interface for LSM and FCS connections please see page 1 9 Baseport bottom for the connection of documentation equipment via the 60 mm interface Binocular phototube with 60 mm interface 3 4 1 Image orientation of camera ports The following table gives
46. 0 Name and intended application Carl Zeiss 1 INSTRUMENT DESCRIPTION 1 1 Name and intended application M anufacturer s name Inverted microscope for transmitted light and epifluorescence Brief name Axiovert 200 manual version Axiovert 200 M motorized version The Axiovert 200 microscopes fit in the product family of inverted transmitted light microscopes as follows Laboratory microscopes Research microscopes Axiovert 25 Axiovert 200 Axiovert 25 C Axiovert 200M Axiovert 25 CFL The Axiovert 200 microscopes are universally applicable inverted microscopes and are mainly used for the examination of cell and tissue cultures and of sediments in culture flasks Petri dishes microtiter plates etc in transmitted and reflected light The Axiovert 200 microscopes permit the performance of the transmitted light techniques brightfield phase contrast differential interference contrast and VAREL contrast and the epi fluorescence technique The microscopes Axiovert 200 and Axiovert 200 M are the basis for scientific microscopic work on living cells The sturdy stand provides attachment possibilities for various tools micromanipulation different light sources temperature control devices etc Ample space is available for the specimens and the relevant handling systems on account of the inverted design the LD illumination system of the microscope and the use of fixed stages This permits the performance of experi
47. 1 39 45 V L2 34 39 V dt Mp 50W DW drole ann ai een 100 h HBO 103 mercury vapor short arc Heer tiat ctus 100W Opto mechanical data Stand with stage e rec tegeret Dec eh ap ilb bea beg at ad with coarse drive 5 mm rot and fine drive 0 1 mm rot Fine scaling 1 um scale part Overall lift approx 10 mm Objective chage eee re D Pas erecta ee via 6 position nosepiece nte kie eel umen Vet uei t e lE with W 0 8 x 1 36 thread Eyepieces ee rer ERDE with plug in diameter 30 mm PM c E field number 23 40 080 03 01 1 15 INSTRUMENT DESCRIPTION Carl Zeiss Technical Data Axiovert 200 1 16 B 40 080 e 03 01 START UP Axiovert 200 Contents START UP Contents 2 START UP 2 1 Unpacking and installation of the 2 2 Attachment of binocular photo 22 1 Inserting the eyepieces and the centering lt 2 2 2 Inserting the eyepiece nnnm nnn 2 3 Attachment of transmitted light illumination 2 3 1 Attachment of carrier for transmitted light illumination 100 W 2 3 2 Attachment of carrier for transmitted light illumination 30 W 2 4
48. 21 OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 Motorized 5 position reflector turret 3 13 3 Accepts the maximum of 5 reflector modules for epi fluorescence Fast change of reflector position through pressing the key forward or backward on the right of the stand 3 13 5 The activated reflector position is marked with a line on the right of the reflector turret The reflector turret can also be operated manually Key REFLECTOR lt gt 3 13 5 Switches the reflector turret forwards or backwards by one position The reflector turret can also be operated manually Key pressed briefly current position is shown in the LCD display for 4 s or until the next press of the key without LCD display the position is approached immediately key pressed again target position is displayed and approached If a position with a filter set is in the beam path the filter set is shown in the lower line of the LCD display The voltage of the halogen illuminator is displayed only as a digit If the halogen illuminator is switched off 0 V is displayed If no filter set has been configured for the reflector position the reflector position is displayed However the filter set and the reflector position are only displayed if the FL fluorescence shutter is open FL is displayed in the upper line of the LCD display FL key on off 3 13 10 Switches the fluorescence shutter on or off alternately pressed briefly 1 s
49. 21 Set MOJE u 3 14 3 23 SIGE POR eg lee 3 12 Sera o rasen 3 12 specimen TOI d BI nee ea Det 3 11 specimen Stage zu etatis up le er ette te eL pe deut 2 11 itj PH 2 5 stop for focusing i sioe 3 6 SWIECDE ON KON 2 19 T POG GIG Re 3 6 transmitted GQ em 3 28 3 32 3 33 3 35 3 38 transmitted light een Se 2 8 ITE 2 6 U UPI PACKING exec ssc te 2 5 V VA REL COM c M 3 38 vertical adjustment of 3 11 3 46 WORK TE 3 18 x en kn Asche 3 7 2 TAGEOUS codes tet Do E nie ED 3 25 ZERO UT 3 18 B 40 080 e 03 01 A 7 Carl Zeiss A 8 ANNEX List of key words Axiovert 200 40 080 e 03 01
50. 3 1 8 Coarse adjustment big knob Focusing knob for coarse adjustment on both sides of the instrument 1 coarse rotation 5 mm Overall lift approx 10 mm Fine adjustment small knob Focusing knob for fine adjustment available on both sides of the instrument 1 fine rotation 0 1 mm Clamping lever for vertical stop of the focusing drive 3 1 33 Setting of vertical stop for stage focusing Turn clamping lever for stop upwards towards the stopping pin Use the focusing drive to move the stage to the uppermost admissible position where collisions with the stage or the microscope slide are no longer possible Then press the clamping lever downwards to clamp it in the stop position 3 6 B40 080e 03 01 OPERATION Axiovert 200 Axiovert 200 Carl Zeiss Nosepiece with objectives 3 1 2 6 position H DIC 3 2 1 with compartments for DIC slider 3 2 2 in all objective positions Fast change of objectives through rotation of the nosepiece via the setting ring 3 2 4 When the heating stage is used the N nosepiece must first be moved to the position via the focusing drive before a new objective is swung in since otherwise collision between the objective and the heating stage might e y K 4 5 Ay Bo Ino Compartment for analyzer slider 3 1 3 and 3 2 3 Fig 3 2 Nosepiece with mounts for DIC For fixed analyzer slider with two dia 32 mm slider filt
51. 45 0000 000 Microscope configurations and modules sheet 1 Object mount for two Petri dishes 35 TV adapter acc price list Binocular phototube with sliding prism 45 23 000000 1005 828 Binocular tube 45 23 000000 1005 827 Binocular ergotube 25 23 000000 1005 829 B40 080e 03 01 INSTRUMENT DESCRIPTION Axiovert 200 Conversion filter 3200 5500 K d 32x2 467847 0000 000 ec Interference wide band filter green d 32x4 467803 0000 000 DIC prism 1 0 55 000000 1005 866 DIC prism 11 0 55 000000 1005 867 DIC prism 0 55 000000 1005 868 LCD display Polarizer D 000000 1005 864 Polarizer D rotatable 000000 1121 813 LD condenser 0 55 H DIC Ph 1 Ph 2 Ph 3 Var 1 2 000000 1005 846 LD condenser 0 55 H Ph 1 Ph 2 Ph 3 DIC DIC 000000 1005 847 DIC prism 11 1 4 445375 0000 000 DIC prism 111 1 4 445376 0000 000 DIC prism 111 0 8 445485 0000 000 Condenser mount for condensers 0 8 1 4 000000 1005 849 Achromatic condenser 0 8 H D Ph DIC 445445 9901 000 007 Achromatic aplanatic condenser 1 4 H D Ph DIC 445453 0000 000 Microscope configurations and modules and light manager 000000 1071 167 Cover glass cup 000000 1016 757 Aqua Stop 000000 1 109 740 Objectives acc price list Antiglare screen for Axiovert 200 000000 1109 741 Cable release 416167 0000 000 Axiovert 200 basic st
52. 6 Axiovert 200 M basic stand 000000 1005 820 AxioVision Control Software 000000 1068 563 RS 233 cable 457411 9012 000 Control PC board with interface 000000 1015 381 contained in basic stand High Resolution Microscopy Camera AxioCam b w 000000 0412 311 High Resolution Microscopy Colour Camera AxioCam Colour 000000 0412 312 Adapter Video V200 C 2 3 0 63x at frontport 000000 1071 171 Tube lens 1 0 for LSM 000000 1071 169 5 position mot reflector turret empty 000000 1064 365 5 position reflector turret empty 000000 1064 364 LSM adapter SP left and sideport 60 right 3 switching positions 000000 1071 164 LSM adapter BP and sideport 60 left 3 switching positions 000000 1071 165 LSM adapter BP and FCS adapter SP left 3 switching positions 000000 1071 166 Safety installations LSM 510 PASCAL 000000 1071 174 Beam path switching mot Beam path switching mot LSM 000000 1069 229 000000 1069 230 If there are no special motorized components all components of the manual stand can also be used at the motorized stand Fig 1 4 Microscope configurations and modules sheet 4 B 40 080 e 03 01 1 9 INSTRUMENT DESCRIPTION Carl Zeiss Objectives Axiovert 200 1 4 Objectives The objectives are the optical centerpiece of the microscope The following is an example of how objectives can be labelled A Plan 10x 0 20 o Key 10x objective magnification with a color ring on the objective bei
53. 7 D 0 7 1 D 20 17 0 6 corr D 0 1 5 corr D 0 1 5 corr D 20 2 corr D 20 2 corr D 0 1 5 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 D 20 17 ph2 var2 000000 1018 589 000000 1020 863 000000 1006 591 000000 1006 592 000000 1006 593 000000 1006 594 000000 1006 595 000000 1006 596 000000 1016 757 440020 0000 000 440031 0000 000 440844 0000 000 440845 0000 000 440864 0000 000 440865 0000 000 440861 0000 000 440320 0000 000 440321 0000 000 440330 0000 000 440331 0000 000 440530 0000 000 440531 0000 000 000000 1004 072 000000 1004 989 440350 9902 000 440351 9902 000 000000 1022 818 000000 1022 819 440364 0000 000 000000 1018 595 000000 1031 171 440760 0000 000 Carl Zeiss 1 5 Eyepieces INSTRUM ENT DESCRIPTION Eyepieces Condensers The following eyepieces are available for the Axiovert 200 and Axiovert 200 M Eyepiece type Eyepiece W PL 10x 23 Br foc Eyepiece W PL 10x 23 Br foc Centering telescope d 30 1 6 Condensers Axiovert 200 The following condensers are available for combination with the stage carrier D and the transmitted light equipment on the Axiovert 200 Axiovert 200 M Condenser type Cat No Comments LD condenser 0 35 5 positions H DIC PhO Ph2 Var1 2 000000 1005 844 from objective 2 5x LD condenser 0 55 6 positions Ph1 Ph2 DIC DIC mot 000000
54. 722 0000 000 Scanning stage 000000 1113 509 1 8 Binocular tubes The following tubes can be used with the Axiovert 200 microscopes Description Cat No VIEWING ANGLE Light distribution in FIELD NUMB ER Binocular tube 45 23 000000 1005 827 45 23 100 Binocular phototube 45 23 000000 1005 828 45 23 100 0 0 100 50 50 with Bertrand lens and shutter Binocular ergotube 25 23 000000 1005 829 25 23 100 vertically variable with Bertrand lens and shutter 40 080 03 01 1 13 INSTRUM ENT DESCRIPTION Carl Zeiss Technical Data Axiovert 200 1 9 Technical Data Dimensions width x depth x height Stand Axiovert 200 cn nennen approx 295 x 805 x 707 mm Weight Axiovert 200 without fluorescence approx 26 kg Axiovert 200 M with fluorescence equipment approx 40 kg Ambient conditions Storage and transport in packaging Permissible ambient temperature uices ee sera nannten 40 to 450 C Operation Permissible ambient 10 to 35 C Permissible relative humidity without 5 max 80 Altitude GT Users een max 2000 m Atmospheric 800 hPa to 1060 hPa Pollution degree ann nenne 2 Operating data Category
55. D A Plan Ph2Var2 3 3 4 3 Setting of VAREL on the Axiovert 200 manual and Axiovert 200 M e Open the aperture diaphragm 3 21 1 completely e Swing VAREL stop on the turret of the condenser into the beam path Var position e Swing in the required VAREL objective via the nosepiece e Use the setting screw 3 21 2 to move the Varel diaphragm opening until optimum VAREL contrast is achieved relief like impression Fig 3 21 Setting of VAREL contrast Microtiter plates Select the opposite VAREL ring of the diaphragm for illumination at the edge of the cavity in the center of the cavity the right or left VAREL ring can be used 3 38 40 080 e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss The specimen field with the relevant pupil image of the VAREL diaphragm is shown in Fig 3 22 In the pupil image the diaphragm appears rotated by 180 Fig 3 22 VAREL contrast with microtiter plates Shifting the VAREL illumination to outside the pupil corresponds to unilateral darkfield illumination Shifting the VAREL illumination between the Ph and VAREL rings of the objective corresponds to oblique brightfield illumination Oblique brightfield Unilateral darkfield FEE var illumination illumination UN A 554 ut Ph ring VAREL ring VAREL diaphragm Fig 3 23 Pupil images in VAREL contrast 40 080
56. Name and intended 1 2 Instrument description and main 1 3 Microscope configurations and 1 4 OBJECTIVES p A 1 5 Eyapieces 1 6 Condehsers vn ak 1 7 Specimen stages and mounting 1 8 seii d 1 9 Technical Data Ascot editus ee 40 080 03 01 Carl Zeiss 0 3 INTRODUCTION Carl Zeiss Contents Axiovert 200 page CHAPTER 2 START UP una RR aka 2 5 2 1 Unpacking and installation of the TiiETOSCO DG oy eet d etd cet en erui cette 2 5 2 2 Attachment of binocular photo 2 6 2 2 1 Inserting the eyepieces and the centering 2 6 2 2 2 Inserting the eyepiece reticle rennen eigen 2 7 2 3 Attachment of transmitted light illumination eenn ne 2 8 2 3 1 Attachment of carrier for transmitted light illumination 100 2 8 2 3 2 Attachment of carrier for transmitted light illumination 30 2 8 2 4 esr Mages i o ee Reese 2 9 2 5 Attachment of microscope 53088 an ae ede da er cid eee enr e eiue ud dass 2 10 2 5 1 Attachment of mecha
57. Operating Manual Axiovert 200 Axiovert 200 M INTRODUCTION Carl Zeiss Copyright Axiovert 200 Knowledge of this manual is required for the operation of the instrument Would you please therefore make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument The specifications are subject to change the manual is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights reserved in the event of granting of patents or registration of a utility model Issued by Carl Zeiss Light Microscopy P O B 4041 37030 G ttingen GERM ANY Phone 49 551 5060 660 Telefax 49 551 5060 464 Internet www zeiss de micro E M ail micro zeiss de Number of this manual B 40 080 e Date of issue 30 03 2001 0 2 B 40 080 e 03 01 INTRODUCTION Axiovert 200 Contents CONTENTS INTRODUCTION nnne CODVETOTIEG ee Indice Es CONTEM S ooa scudo IE Notes on instrument 5 Notes Warrants en Overall view of the Axiovert 200 Overall view of the Axiovert 200 Microscopy in transmitted light brightfield in a few steps CHAPTER 1 INSTRUMENT DESCRIPTION nnne 1 1
58. These partial beams pass two neighboring specimen areas at a small distance and experience different path differences on account of different refractive indices and specimen thicknesses Both beams are then united in a second birefringent prism and feature the same vibration direction after passing the analyzer Therefore both partial beams can interfere in the intermediate image with the path differences being changed to different gray values intensities 3 3 3 2 Configuration of the Axiovert 200 manual and Axiovert 200 M Objectives offered with the DIC equipment e g Plan Neofluar DIC slider suitable for the objectives used Condenser with turret equipped with DIC prisms DIC 1 DIC Il DIC III Polarizer e g model D with 2 position filter changer Analyzer e g fixed analyzer slider or analyzer slider 30 de S narmont 3 3 3 3 Setting of transmitted light DIC on the Axiovert 200 manual and Axiovert 200 M e Swing in the suitable DIC objective Push appropriate DIC slider 3 20 3 into the compartment on the nosepiece M ake sure that the DIC slider engages in the stop position e Swing in the suitable DIC prism I Il or Ill digit on condenser turret e Push analyzer slider 3 20 4 into the stand Ensure the correct stop position 1 Transmitted light DIC with fixed analyzer slider e Switch on polarizer 3 20 1 on the carrier for transmitted light illumination Ensure the correct stop position e Place a spec
59. a detailed overview of the image orientation on the Axiovert 200 camera ports The descriptions are based on an object such as a stage micrometer with readable figures or letters to illustrate the orientation F Original object Viewed without microscope P As shown above the object is placed on the microscope stage with the readable side facing the objective and is then seen from top by the microscope user as follows This view is the reference view for the description below q Viewed from top too a movement of the specimen stage in Y direction to the back thick arrow and in X direction thin arrow appears like this ae For a given viewing direction related to the respective stand component the table below provides the following information Intermediate image of the object and the image on the monitor as captured by the camera as well as direction of motion of the object when moving the stage 40 080 03 01 3 43 OPERATION Carl Zeiss Documentation Axiovert 200 Order No Viewed with Intermediate Direction of Viewing direction Description Switch position image Monitor motion of object schematic 000000 1005 827 Eyepieces 100 vis Binocular tube q 000000 1005 828 100 vis 0 doc Binocular phototube 50 vis 50 doc 0 vis 100 doc 000000 1005 829 Eyepieces 100 vis Binocular ergotube 000000 1005 815 20 vis 80 doc Sideport 60 left 2 switching positions
60. a ee ir Peres 3 16 Operation and function controls on the Axiovert 200 motorized 3 16 Switching on and basic settings on the Axiovert 200 M 3 23 umination and contrasting techniques scu cna oct engrais nennen 3 28 Setting of transmitted light brightfield for KOHLER illumination 3 28 Setting of transmitted light phase contrast 3 33 Setting of differential interference contrast DIC in transmitted light 3 35 Setting of VAREL contrast in transmitted light siii nn 3 38 Setting of fluorescence contrast in reflected light 3 40 Documentations inise m MM 3 43 Image orientation of camera DOTES re 3 43 Photomicrography with SLR Camaeta ero eren eh ar aha 3 45 Photomicrography using a digital camera and 3 46 40 080 03 01 3 1 Carl Zeiss OPERATION List of illustrations Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 3 2 1 1 NJ NJ NJ NJ I IS r3 rH O Uil 4 HA Uni UJ QO Ui 4 OU hJ EA CO List of illustrations Axiovert 200 Operation and function controls the Axiovert 200 3 4 Nose
61. a list of filter sets to be scrolled through upwards or downwards e If the correct filter set for the relevant reflector turret position appears in the LCD display the next turret position can be swung in by pressing one of the two REFLECTOR keys and the relevant filter set can be assigned Repeat the procedure for all reflector turret positions Then change to the next programming item or exit the Set mode 3 24 40 080 e 03 01 OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss Setting of Z focus factors and parfocality The matching of focus positions parfocality and the adaptation of the focus speed Z focus factors to the objective magnification are normally only performed when the microscope is used for the first time or after the change of objectives in the nosepiece The parfocality values are automatically saved during setting of the Z focus factors To correctly save the parfocality values the objective with the highest magnification must be used to focus on a specimen before the Set mode is entered e Place a specimen on the stage Swing in dry objective with the highest magnification e Focus on the specimen via the fine adjustment e Activate Set mode by pressing key Set LM for more than 2 seconds e Press one of the two FOCUS keys Change to focus programming is made and SET FOC SPEED 6 is displayed in the upper line of the LCD display The number 6 stands for the focus factor currently set The set nosepiece p
62. agm iris stop is opened completely when a phase stop position is switched on When a new turret position is switched on the aperture diaphragm is automatically reset to its last opening 3 10 40 080 e 03 01 OPERATION Axiovert 200 Axiovert 200 Carl Zeiss Centering screw for condenser 3 1 18 and 22 Screws for condenser centering attached to both sides of the carrier for transmitted light illumination condenser Lever for luminous field diaphragm transmitted light 3 1 20 Lever attached to the carrier for transmitted light illumination condenser which opens and closes the transmitted light luminous field diaphragm to set KOHLER illumination Lever in front position luminous field diaphragm fully opened Lever in rear position luminous field diaphragm closed Lever for vertical adjustment of the condenser 3 1 21 Setting knob attached to the carrier for transmitted light illumination condenser which raises and lowers the condenser to set KOHLER illumination Polarizer D with 2 position filter changer 3 1 17 The polarizer and two filter positions can be swung in and out separately Stop position in swung in status Microscope stages with specimen holder 3 1 24 The specimens are mounted positioned and fixed in position using the specimen holder Equipped as follows depending on the instrument configuration specimen stage 250x230 with object guide and mounting frame for object gui
63. al value When the unit is switched off the message HAL OFF appears on the LCD display Long pressing 1 s automatically sets the brightness to 3200K for color photography Motorized focusing drive 3 13 6 Focusing knob available on both sides of the instrument Coarse adjustment big knob Fine adjustment small knob 40 080 03 01 3 17 OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 FOCUS key Work Load 3 13 8 Load key Brings the nosepiece to the lowest position Load Position appears in the lower line of the LCD display The lower LCD line blinks every second as a warning sign Work A key pressed briefly 0 9 s travel to work position if in load position pressed long gt 2 5 setting of focus limit ZERO Y key 3 13 7 Key pressed briefly current focus position is shown in the LCD display activation of meauring mode key pressed long 2 S Z position is zeroed Assoon asthe Z drive is moved the current focus position is displayed in um with one digit after the comma negative sign for lowering of nosepiece from the zero position positive sign for moving upwards from zero position Exiting of measuring mode after objective change or Optovar change Motorized nosepiece with objectives 3 13 2 Motorized 6 position nosepieces H DIC with compartments for DIC sliders in all objective positions Fast change of objectives through pressing the key on t
64. and 000000 1005 813 Adapter V200 T2 2 5x for SLR at frontport 000000 1005 869 Single lens reflex camera housing u x 9 9 000000 1057 127 T2 adapter for Contax Contax Bayonet 416010 0000 000 xe Video V200 C 2 3 0 63x at frontport 000000 1071 171 High Resolution Microscopy Camera AxioCam b w 000000 0412 311 High Resolution Microscopy Colour Camera AxioCam Colour 000000 0412 312 Baseport 60 000000 1005 824 Path deflection to the tube 000000 1069 227 Beam path switching 000000 1069 228 Fig 1 2 Microscope configurations and modules sheet 2 40 080 03 01 Carl Zeiss 100 HAL lamp housing with collector lamp mount 447219 0000 000 12 V 100 W Halogen lamp 380079 9540 000 Carrier for transmitted light illumination and condenser 000000 1005 840 Control PC board 000000 1015 348 p opeope mpo Control PC board with interface 000000 1015 381 Analyzer slider fixed 000000 1005 862 Analyzer slider 30 000000 1005 863 slider acc price list Optovar 1 6x 000000 1005 855 Optovar 2 5x 000000 1005 856 Double video adapter 000000 1058 640 Sideport 60 left 2 switching positions 000000 1005 815 Sideport 60 left 3 switching positions 000000 1005 816 Sideport 60 right 3 switching positions 000000 1005 817 Sideport 60 left and right 3 switching positions 000000 1005 818 1 7 Carl Ze
65. and cooling body out of the mount in the lamp base 2 31 2 The wire connection need not be detached Loosen fixation screw 2 31 5 on the cooling body 2 31 6 and remove cooling body If the cooling body cannot be removed or attached unscrew fixation screw entirely screw it into the adjacent drilled hole and press on the cooling body Dispose of defective lamp in accordance with the regulations Push new lamp into the cooling body until stop with the smaller diameter Ensure the correct hole diameter for insertion and do not exert any force on the lamp The mount in the cooling body is marked with H for HBO or X for XBO Clamp the fixation screw on the cooling body Insert new lamp 2 31 1 with cooling body into the mount in the lamp base 2 31 2 with the spring lever being pressed down 2 31 4 Make sure that the lead to the cooling body remains accessible Release spring lever Do not exert any force on the lamp Attach lamp housing to lamp base 2 31 3 and tighten clamping screw 2 30 2 B40 080e 03 01 START UP Axiovert 200 Illuminator N HBO 103 Carl Zeiss 2 13 2 Coarse alignment of illuminator N HBO 103 The following settings for adjustment of the N HBO 103 illuminator are performed in two steps the coarse adjustment and the subsequent fine adjustment using the adjustment aid During coarse adjustment UV protection goggles must be worn to protect the eyes from UV radiation
66. and allow it to heat to operating temperature for approx 15 minutes Select the FL reflector module in the reflector turret containing the required fluorescence filter combination depending on the excitation and switch it on Press the FL on off key 3 24 6 to open the fluorescence shutter The opening of the shutter is confirmed by a beep For the epi fluorescence technique one slider each with iris stop is used as aperture and luminous field diaphragm However since the aperture diaphragm is not visible in reflected light the aperture diaphragm slider must first be centered in the compartment of the luminous field diaphragm and then be inserted in the compartment for the aperture diaphragm The centering for the aperture diaphragm and the luminous field diaphragm is performed in the same way Insert an iris stop slider into the compartment 3 24 3 for the luminous field diaphragm until stop Use the lever to close the diaphragm until it becomes visible in the field of view Then center the diaphragm using the two adjusting screws in the slider with SW 3 mm ball headed screw driver Open the diaphragm until the entire field is uncovered Push the slider centered in this way into the compartment for the aperture diaphragm 3 24 2 until stop Insert an additional iris stop slider into the compartment 3 24 3 for the luminous field diaphragm Close the luminous field diaphragm until it becomes visible in the field of view Use the
67. and burns The HBO 103 W 2 lamp may only be removed from the packaging and inserted in the N HBO 103 illuminator if a protection mask and safety gloves are worn electrical clamping connections must be made carefully Pronounced heat during operation may result in loose contacts For start up or after expiry of the average operation time of 300 h the HBO 103 W 2 mercury vapor short arc lamp must be inserted or exchanged The operating time of the lamp can be read from the counter 2 29 1 of the ebq 100 dc power supply Follow the following steps when inserting changing the HBO 103 W 2 lamp e Pull off or unscrew line cable near 2 29 2 and N HBO 103 connector near 2 29 3 from the ebq 100 dc power supply e Wear protection mask and safety gloves Fig 2 29 Power supply ebq 100 dc B 40 080 e 03 01 2 23 START UP Illuminator N HBO 103 Carl Zeiss Fig 2 30 Removal of N 103 housing Fig 2 31 Changing the HBO 103 W 2 mercury pressure short arc lamp 2 24 200 Use focusing knob 2 30 3 to bring collector to the position at the very front in the direction of the light Use ball headed screwdriver to loosen clamping screw for lamp housing 2 30 2 and carefully remove lamp housing 2 30 1 from the lamp mount 2 31 3 in an upward direction Press spring lever 2 31 4 downwards and remove defective lamp or transport lock
68. be 2 35 1 e Insert dovetail of lamp housing in reflected light tube 2 35 1 on the instrument rear and use SW 3 ball headed screwdriver to tighten clamping screw 2 35 2 e Insert multi pin plug of the N 103 illuminator 2 35 4 into the instrument socket 2 36 2 of the power supply 100 dc and secure it with captive coupling ring if not already done so after adjustment e Connect the line cable to the line socket 2 36 1 of power supply ebq 100 dc first and then to the line if not already done so after adjustment Fig 2 35 Attachment of illuminator N HBO 103 Fig 2 36 Powersupply 100 dc 2 26 B 40 080 e 03 01 START UP Axiovert 200 Illuminator N HBO 103 Carl Zeiss 2 13 4 Fine adjustment of illuminator N HBO 103 using the adjusting aid Attach illuminator N HBO 103 to the microscope stand tighten the clamping screw and switch on the illuminator e Pull out adjusting aid 2 37 7 from the incident light tube The brighter focal spot of the HBO 103 W 2 lamp and its slightly darker reflector image become visible in the black glass window of the adjusting aid e Use knurled knob 2 37 6 to focus on the brighter focal spot via collector adjustment e Appl SW 3 ball headed screwdriver on adjusting screw 2 37 3 to focus on the slightly darker reflected focal spot of the lamp e f required set knurled knob 2 37 6 and adjusting screw 2 37 3 repeated
69. be Carl Zeiss 2 2 2 Inserting the eyepiece reticle The eyepieces W PL 10x 23 Br foc are intended for use with eyepiece reticles The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot 2 4 W but the red dot 2 4 R The eyepiece reticles 2 4 1 have been adhered to screw in mounts 2 4 2 by the manufacturer to allow easy replacement To replace an eyepiece reticle remove screw in Fig 2 4 Inserting the eyepiece reticle mount 2 4 2 with eyepiece reticle 2 4 1 and replace it with a screw in mount containing the required eyepiece reticle If eyepiece reticles are inserted into the unscrewed mount by the customer attention must be paid to the labelling being visible the right way up after insertion Compensation of ametropia when eyepiece reticles are used For the correct use of an eyepiece reticle two focusing eyepieces are required to allow the user to compensate for differences in the visual performance of his two eyes e Use the focusing eyepiece to focus on the eyepiece reticle focus on the edge of the field of view if no eyepiece reticle is used e Use the focusing drive to focus on the microscope image of a specimen through the eyepiece set as described above e Then use the second focusing eyepiece to focus the microscope image for the second eye The positi
70. ching positions with different beam splitting ratios 2 switching positions with instrument configuration with Sideport 60 left 100 vis 0 96 doc 20 96 vis 80 doc left 3 switching positions with instrument configuration with Sideport 60 left 100 vis 0 96 doc 0 vis 100 doc left 50 96 vis 50 doc left 3 switching positions with instrument configuration with Sideport 60 right 100 vis 0 96 doc 0 96 vis 100 doc right 50 96 vis 50 doc right 3 switching positions with instrument confi 100 vis 0 96 doc 0 vis 100 doc left 20 96 vis 80 doc right e DOOF HHO uration with Sideport 60 left and right Oa 3 12 B40 080e 03 01 Axiovert 200 OPERATION 5 position reflector turret 3 1 7 Accepts the maximum of 5 reflector modules for epi fluorescence Fast change of reflector position by turning the setting ring of the reflector turret The activated reflector position is marked with a line 3 9 1 on the right of the reflector turret FL key on off 3 1 9 Switches the fluorescence shutter on or off alternately FL in the LCD display if available indicates that the shutter has been opened HBO XBO adjusting aid for lamp adjustment 3 1 25 The adjusting aid is used to adjust and center the N HBO103 mercury vapor short arc lamp Axiovert 200 Carl Zeiss Fig 3 9 5 position reflector turret The adjusting aid is activated deactivated by pulling o
71. cially authorized personnel Should any defect occur with the instrument please get in touch with the Carl Zeiss microscopy service in Germany see page 4 6 or your local Zeiss agency 0 10 B 40 080 03 01 INTRODUCTION Overall view of the Axiovert 200 and Axiovert 200 M Carl Zeiss Axiovert 200 Overall view of the Axiovert 200 manual Overall view of the Axiovert 200 M motorized 0 11 40 080 03 01 INTRODUCTION Carl Zeiss Microscopy in transmitted light brightfield in a few steps Axiovert 200 Microscopy in transmitted light brightfield in a few steps D Before starting to use the Axiovert 200 make sure to read the notes on instrument safety and the chapters entitled Instrument Description Chapter 1 and Start up Chapter 2 e Make the microscope ready for operation as described in chapter 2 and switch it on via the On Off switch 0 2 1 e Select the objective with the lowest magnification e g 10x on the nosepiece 0 2 2 Set factor 1x on the setting wheel 0 2 4 of the Optovar turret Open the luminous field diaphragm or the aperture diaphragm completely by pulling lever 0 2 16 to the front until stop or by turning the setting wheel 0 2 20 to the front until stop Turn the setting ring 0 2 19 to move the condenser turret in position H for brightfield or DIC e Move reflector turret 0 2 5 if available into the position without filter combination via the setting ri
72. contrast DIC in transmitted light 3 3 4 Setting of VAREL contrast in transmitted light 3 3 5 Setting of fluorescence contrast in reflected 3 4 Documentation anne enable 3 4 1 Image orientation of Camera Hide 3 4 2 Photomicrography with SLR 3 4 3 Photomicrography using a digital camera and videomicroscopy 40 080 03 01 Carl Zeiss 0 5 Carl Zeiss CHAPTER 4 4 1 4 2 4 2 1 4 2 2 4 2 3 4 3 0 6 INTRODUCTION Contents Axiovert 200 page CARE MAINTENANCE TROUBLESHOOTING AND 4 3 4 3 Mantente Metern este cordis Pier tA OE dm 4 4 Performing CHECKS ases coco pt ee Neil 4 4 Changing the fuses the 4 4 Changing the fuses on the 100 dc 4 5 ea E 4 6 ANNEX En cepe Gra n EE CODO RE ER GERE RUE GO RD ER ER HA E A 1 GDDIEVIAHONS ie een nat seat oo E erie laf euere A 3 List OT Key Se tian tort Est A 5 Certification in accordance with DIN ISO 9001 EN 46001 EC Declaration of Conformity B40 080e 03 01 INTRODUCTION Axiovert 200 Notes on instrument safety Carl Zeiss Notes on i
73. de M mechanical stage 250x230 and mounting frame for mechanical stage K scanning stage heating stage gliding stage Z Key Set LM 3 1 35 Stores the Light Manager values and enters the configuration mode Frontport 3 1 12 Port forthe connection of documentation equipment Clamping screw for Frontport 3 1 31 Clamping screw for fixation of the camera video adapter to the Frontport 40 080 03 01 3 11 OPERATION Carl Zeiss Axiovert 200 Axiovert 200 Baseport 3 1 34 Port forthe connection of documentation equipment below the Axiovert 200 Setting knob for Frontport Baseport vis 3 1 32 Selects the beam splitting ratio for Frontport Baseport and visual observation 3 switching positions 100 vis free light path vs 100 Frontport EE 100 Baseport Sideport right 3 1 5 Port for the connection of documentation equipment Different splitting ratios for Sideport right Sideport left and visual observation vis depending on the instrument configuration Sideport left 3 1 29 Port for the connection of documentation equipment Different splitting ratios for Sideport right Sideport left and visual observation vis depending on the instrument configuration Setting wheel for Sideport right Sideport left vis 3 1 30 Selection of beam splitting for Sideport doc right Sideport doc left and visual vis observation 2 or 3 swit
74. e rings Ph 0 Ph 1 Ph 2 Ph for different average numeric apertures which can also be used in brightfield without any restriction Condenser with turret containing centering phase stops Ph 0 Ph 1 Ph 2 and Ph 3 for various medium numeric apertures The activated phase stop on the condenser must match with the relevant description on the objective e g Ph 1 40 080 03 01 3 33 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3 3 2 3 Setting of transmitted light phase contrast on the Axiovert 200 Axiovert 200 M e Swing phase contrast objective e g Ph 1 into the beam path e Switch phase stop with the same description as the phase contrast objective e g Ph 1 in the turret of the condenser e To check the centering and congruence of the bright phase stop in the condenser with the dark phase ring in the objective remove one Fig 3 18 Centering the phase stop on the eyepiece from the tube and replace it with the condenser centering telescope Use the correction facility of the centering telescope to focus on the phase stop and the phase ring in the objective exit pupil When the phototube is used the Bertrand lens can also be switched on for observation of the Objective exit pupil When the Bertrand lens is used the factor 1x must be set on the setting wheel of the Optovar turret e f congruence is not perfect 3 19 A two SW 15 Allen keys 3 18 1 must b
75. e used to recenter the bright phase stop until complete congruence with the dark phase ring has been achieved 3 19 B e Finally remove the centering telescope from the tube and replace it with an eyepiece or Fig 3 19 Centering of phase stop bright in switch off the Bertrand lens condenser with phase ring dark in objective However centering is normally not required since the phase stops are centered in the factory To enhance the image contrast an interference wide band filter green 32 x 4 can be inserted in the filter changer Complete phase contrast is only achieved if the bright phase stop in the condenser and the dark phase ring in the objective are exactly congruent in the illumination beam path 3 19 B D Adjustment of the phase rings must be performed for all phase contrast objectives used When liquid objects are examined in small vessels the beam path must be aligned to the center of the vessel since liquids on the edge of a vessel have the same effect as lenses and impair the microscope image 3 34 B40 080e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss 3 3 3 Setting of differential interference contrast DIC in transmitted light 3 3 3 1 General principle The transmitted light DIC technique permits high contrast 3D images of transparent specimen details Light which has been linearly polarized by a polarizer is split into two partial beams in a birefringent prism
76. eel for Sideport Setting knob for Frontport Baseport 0 13 INTRODUCTION Carl Zeiss Microscopy in transmitted light brightfield in a few steps Axiovert 200 0 14 40 080 e 03 01 INSTRUMENT DESCRIPTION Axiovert 200 Contents List of illustrations Carl Zeiss INSTRUMENT DESCRIPTION Contents 1 INSTRUMENT DESCRIPTION e lv menu 1 3 1 1 Name and intended applications ses eno v ratu e cal es ne d ra 1 3 1 2 Instrument description and main features aus HAIE HE 1 5 1 3 Microscope configurations and 1 6 1 4 i a rue ee ale leeren 1 10 1 5 als cH 1 12 1 6 COIDdelsers c soceri ee 1 12 1 7 Specimen stages and mounting 1 13 1 8 m THU ee 1 13 1 9 1 14 List of illustrations Fig 1 1 Microscope configurations and modules sheet 1 1 6 Fig 1 2 Microscope configurations and modules sheet 2 1 7 Fig 1 3 Microscope configurations and modules sheet 3 ssssssee ee 1 8 Fig 1 4 Microscope configurations and modules sheet 4 1 9 40 080 03 01 1 1 INSTRUM ENT DESCRIPTION Carl Zeiss Contents List of illustrations Axiovert 200 1 2 40 080 e 03 01 INSTRUMENT DESCRIPTION Axiovert 20
77. er on the carrier for transmitted light illumination with its dovetail pointing upwards Make sure that the orientation pin of the condenser is positioned at the front and exactly engages into the guiding groove of the condenser carrier e Fix condenser adapter in position with clamping screw 2 14 2 e Then insert the required condenser into the condenser adapter via the dovetail making sure that the orientation is correct and fix it into position with clamping screw 2 14 3 2 6 3 Changing the DIC prism in the condenser turret To change a DIC prism remove the condenser and place it upside down on a sturdy support to make the underside accessible e Remove the plastic cover 2 15 1 from the assembly opening 2 15 3 e Position the turret disk containing the DIC prism to be exchanged in the assembly opening and hold it on the knurled ring e Use mounting device from the tool set to unscrew retainer ring 2 15 2 Turn condenser upside down and allow DIC prism 2 15 4 to slide out onto a soft surface Installation of the DIC prism is made in reverse order e Carefully insert new DIC prism into the assembly opening with the labeling pointing Fig 2 15 Changing the DIC prism upwards If required use tweezers to hold the DIC prism carefully on its outer ring Take special care of the correct orientation of the DIC prism in the mount groove of the DIC prism must engage in the pinion of the mount Carefully
78. er positions or analyzer slider 30 for DIC Drive controls for XY adjustment of the mechanical stage or the object guide if specimen stage 250x230 3 1 4 is attached Upper drive knob adjustment in Y direction Lower drive knob adjustment in X direction Setting wheel for Optovar turret 3 1 6 Maximum of 3 switching positions with tube lens 1x optovar lens 1 6x optovar lens 2 5x In the case of equipment with less than 3 lenses the empty positions are blocked 40 080 03 01 3 7 OPERATION Carl Zeiss Axiovert 200 Axiovert 200 Binocular tubes 3 1 13 The binocular tubes offered permit the individual setting of the interpupillary distance and the viewing height within defined limits Binocular tube 45 23 with manual shutter vis 3 3 Shutter switched on off via rotary knob 3 3 1 100 vis 096 vis light shutter WO 1 Fig 3 3 Binocular tube 457 23 Binocular phototube 457 23 with sliding prism for vis doc Bertrand lens and manual shutter vis 3 4 Shutter switched on off via rotary slider knob 3 3 1 100 vis 0 vis light shutter Bertrand lens B Focusing of Bertrand lens through activation of rotary slider knob with the Bertrand lens being switched on Switching of beam path sliding prism vis doc Fig 3 4 Binocular phototube 45723 50 vis 50 96 Ce B 4 100 vis 090 doc gt
79. for activation of the Sideport to the right or left 10096 Frontport port pointing to the front for photomicrography or 10096 Baseport port pointing downwards Since this is a 100 switching position simultaneous visual observation during photography is not possible The 2 5x camera adapter and the camera specific T2 adapter permit commercially available 35 mm SLR cameras to be connected to the microscope e Remove dust cover or camera lens from the camera housing 3 25 1 e Attach appropriate T2 adapter 3 25 2 to the camera housing e Remove dust cover from the 2 5x camera adapter 3 25 3 e Screw 2 5x camera adapter into the T2 adapter via the thread e Remove dust cover from the camera port Fig 3 25 Connection of a SLR camera 40 080 03 01 3 45 OPERATION Carl Zeiss Documentation Axiovert 200 e Attach the premounted camera system to the Frontport align it horizontally and tighten clamping screw 3 25 5 using the SW 3 ball headed screw driver e Switch on the microscope and when using a color film artificial light set the toggle switch for illumination intensity 3 25 4 to 10 5 V corresponds to the color temperature of 3200 K The voltage value can be directly read from the LCD display To make this setting easier for you the increase of the lamp voltage stops at 10 5 V when the toggle switch is pressed down completely and a beep is emitted Should the illumination intensity be too hi
80. gh for the specimen an attenuation filter must be used e Select the object area to be photographed via the binocular tube To determine image section and for exact focusing of the image the adjusting eyepiece should be equipped with the dia 26 MC 10x photo reticle see section 2 2 2 e After switching the beam path 3 25 6 from visual observation to the Frontport 100 of the light is now available for the camera e To operate the camera system please observe the manufacturer s manual is The magnification on the film is the product of objective magnification Optovar magnification and the factor 2 5 of the camera adapter 3 4 3 Photomicrography using a digital camera and videomicroscopy e The Axiovert 200 and Axiovert 200 M permit the connection of one video camera or one digital camera e g AxioCam digital camera from Zeiss 3 26 1 to the Frontport via the 0 63 C mount video adapter 3 26 2 e Attachment of the video adapter is performed in the same way as the camera adapter described in section 3 4 2 Fig 3 26 Video attachment 3 46 B 40 080 e 03 01 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axiovert 200 Contents List of illustrations Carl Zeiss CARE MAINTENANCE TROUBLESHOOTING AND Contents 4 CARE MAINTENANCE TROUBLESHOOTING AND 4 3 4 1 ME CM mp RCM SE RATE DEC CE EINE 4 3 4 2 Maintenante see
81. he contact area and tighten three hexagonal screws from above Make sure that the screws go through the drilled hole of the relevant spacer disk e Then connect the instrument to the power supply as described in the separate operating manual Fig 2 12 Attachment of heating stage When the heating stage is used the nosepiece must first be moved to the lowest position via B the focusing drive before a new objective is swung in since otherwise collision between the objective and the heating stage might occur 2 5 5 Attachment of gliding stage Z The gliding stages are attached in the same way as the heating stage by using three spacer disks e Before attaching the gliding stage to the stand the three support elements on the underside of the gliding stage must be turned out e Place spacer disks on the three contact points of the stand e Place the heating stage on the contact area and tighten three hexagonal screws from above Make sure that the screws go through the drilled hole of the relevant spacer disk When the heating stage is used the nosepiece must first be moved to the lowest position via N the focusing drive before a new objective is swung in since otherwise collision between the objective and the heating stage might occur D After stage assembly cover the drilled holes visible from above with the caps supplied Tilt carrier for transmitted light illumination forward again to move it in its work position 2
82. he right of the stand 3 13 4 The nosepiece can also be operated manually When the heating stage is used the nosepiece must first be moved to the lowest position via the focusing drive before a new objective is swung in since otherwise collision between the objective and the heating stage might occur OBJECTIVE 4 gt key 3 13 4 Switches the nosepiece forwards or backwards by one position The nosepiece can also be operated manually Before turning the nosepiece to the next position move it to its lower end position After the change the previous Z position is approached again and the previous lamp voltage value is reset Lowering is not required if the nosepiece is turned manually The position number magnification and contrasting technique of the current position is shown in the upper line of the LCD display 3 18 B40 080e 03 01 OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss LCD display 3 13 17 Two line display with 16 characters each attached to the carrier for transmitted light S illumination condenser N Display of objective position 3 6 1 magnification 3 6 2 and contrasting technique E 3 6 3 in the upper line a FL display 3 6 4 on the right of the top line if the fluorescence shutter is opened Sy Display of lamp brightness in a line scale and display of the relevant voltage 3 6 5 in the lower line One interval corresponds to 0 4 V The LCD background illu
83. imen on the stage e Set luminous field diaphragm and aperture diaphragm on the condenser 3 20 2 to K HLER illumination e Use knurled screw on the DIC slider to set optimum contrast The symmetric adjustment of the DIC slider around its center position permits specimen details to be shown in 3D as if they were raised or lowered 40 080 03 01 3 35 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 2 Transmitted light DIC with analyzer slider 30 de SENARM ONT When the analyzer slider 30 is used the DIC slider must be brought to the center position first e Swing in the polarizer and bring analyzer 30 3 20 5 in 0 position dark position polarizer and analyzer are crossed e Swing out the DIC prism on the condenser turret use brightfield or phase contrast position for example e Remove one eyepiece and replace it with the centering telescope or switch on Bertrand lens on the phototube lf the field is viewed using the centering telescope or Bertrand lens a diagonal black line of the DIC slider becomes visible from the left top to the right bottom e Adjust the knurled screw on the DIC slider to move the diagonal black line to the center of the field of view e Remove the centering telescope and insert the eyepiece again or switch off Bertrand lens e Swing in DIC position on the condenser e Place a specimen on the stage e Use the setting wheel to turn the analyze
84. ing 3 32 B40 080e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss 3 3 2 Setting of transmitted light phase contrast 3 3 2 1 General principle The phase contrast technique is ideal for examinations of thin unstained specimens e g culture cells The human eye is unable to recognize phase differences differences in refractive index and thickness between the various cell components The phase contrast technique uses the optical modulators phase stop and phase ring and the interference procedures during the formation of the intermediate image to change the small phase differences in intensity and color differences which are visible to the human eye The high intensity direct light components are attenuated using the annular channel optically defined as phase stop and phase ring and a constant phase shift is applied The indirect light components diffracted at various cell components however by pass this optical channel and are influenced by the in phase refractive index and the thickness differences of the specimen In the intermediate image plane the differently influenced partial beams interfere and are enhanced or attenuated depending on the phase position This interference results in image contents displaying intensity and color differences which can be recognized by the human eye 3 3 2 2 Configuration of the Axiovert 200 manual and Axiovert 200 M Phase contrast objectives with phas
85. iss INSTRUMENT DESCRIPTION Phase stop Ph 0 0 22 451261 0000 000 Phase stop Ph 1 0 2 Diaphragm Var 1 0 4 451251 0000 000 Diaphragm var 1 0 55 451252 0000 000 Diaphragm Var 2 0 55 451253 0000 000 451260 0000 000 Phase stop Ph 0 0 4 451255 0000 000 Phase stop Ph 1 0 4 451256 0000 000 Phase stop Ph 2 0 4 451257 0000 000 Phase stop Ph 1 0 55 451258 0000 000 Phase stop Ph 2 0 55 451259 0000 000 Slider Ph H Var 451246 0000 000 Slider Ph H Ph 451245 0000 000 Condenser 0 2 451236 0000 000 Condenser 0 55 451237 0000 000 Condenser 0 4 451235 0000 000 Fig 1 3 1 8 Adjusting aid for HBO XBO 452369 0000 000 Path deflecting mirror for two illuminators 447230 9901 000 Iris stop slider for incident light equipment 000000 1062 360 FL incident light equipment 000000 1066 230 Protection filter for reflector turret 000000 1068 113 5 position reflector turret empty 000000 1064 364 Reflector module Pol P amp C 000000 1046 279 Reflector module Fl P amp C 000000 1046 281 Analyzer module D P amp C 000000 1050 958 Power supply 12 V DC 100 W stabilized 000000 1128 574 Microscope configurations and modules Axiovert 200 Carrier for 6V 25 30W transmitted light illumination 451380 0000 000 transmitted light illumination 000000 1005 842 Power supply unit for N HBO 103 90 250V 50 60Hz 265 VA 000000 1003 928 i Adapter for ca
86. ly until both focal spots have the same dimension X Use adjusting screws 2 37 4 and 5 to adjust the brighter focal spot in the adjusting circle Fig 2 37 Adjusting aid and illuminator N e g on the left side in accordance with the HBO 103 focal spot image 2 37 8 Use adjusting screws 2 37 1 and 2 marked with a dot to adjust the darker focal spot in the adjusting circle e g on the right side in accordance with the focal spot image e Push in adjusting aid again 8 The two focal spots of the HBO 103 W 2 lamp must not superimpose in the adjusting circle of the adjusting aid If required the above settings for fine adjustment of the N HBO 103 illuminator can be performed repeatedly 40 080 03 01 2 27 START UP Carl Zeiss Attachment of Aqua Stop Axiovert 200 2 14 Attachment of Aqua Stop Aqua Stop can be used to protect the objective and the nosepiece from liquids e Remove the microscope stage Put collecting trough 2 38 5 onto nosepiece carrier 2 38 2 and screw it on by means of two screws 2 38 1 Screw the objectives to be used into the nosepiece Fig 2 38 Attachment of Aqua Stop e Then use a sharp pointed scalpel to cut the holes into cover mat 2 38 4 Four hole sizes are available that are pre embossed on the underside of the cover mat and labeled from inside to outside with hole size number 1 to 4 The position ID s of the hole
87. m 0 2 20 to approx 2 3 of the diameter of the objective exit pupil 0 1 E Optimum contrast setting is dependent on the respective specimen e Insert the eyepiece again or swing out Bertrand lens and refocus if required via the fine drive e After the microscope has been set to transmitted light brightfield in this way changing to this special contrasting technique is now possible see chapter 3 of this manual 0 12 40 080 e 03 01 Axiovert 200 On Off switch Objective nosepiece Compartment for slider Analyzer Setting wheel for Optovar turret Reflector turret Focusing drive coarse fine HAL on off switch Toggle switch for illumination intensity Turning or sliding knob for vis doc beam splitting 0 2 Axiovert 200 40 080 03 01 INTRODUCTION Microscopy in transmitted light brightfield in a few steps Carl Zeiss Turning or sliding knob for Bertrand lens and manual shutter Binocular tube component Setting ring of the eyepiece Eyepiece Polarizer D with 2 position filter changer Centering screw for condenser Setting lever for luminous field diaphragm Setting knob for vertical adjustment of the condenser 24 B 2 AI 2 Centering screw for condenser Turret disk of condenser Setting wheel for aperture diaphragm on the condenser Microscope stage Compartment for aperture diaphragm slider Setting wh
88. ments which would not be possible with upright microscopes The design allows the easy attachment of cameras lasers specific stages etc 40 080 03 01 1 3 INSTRUMENT DESCRIPTION Carl Zeiss Name and intended application Axiovert 200 Typical fields of application Observation of intracellular processes in living cell cultures cell cell interactions motility growth measurements of potential drug detection microinjection IVF in vitro fertilization toxicity examinations patch clamp technique ion measurements digital recording long time time lapse examinations in combination with the automation of processes z sectioning deconvolution visualization of molecular structures Fura Ca measurement GFP optical tweezers and scissors single molecule detection TIRF Accessories for temperature control and incubation are described in the separate manual B 40 610 d e Incubation systems on the Axiovert 1 4 40 080 e 03 01 INSTRUMENT DESCRIPTION Axiovert 200 Instrument description and main features Carl Zeiss 1 2 Instrument description and main features The Axiovert 200 Axiovert 200 M is available either as manual or as motorized version The accessory components are part of a modular system For documentation purposes the Axiovert 200 Axiovert 200 M can be equipped with the maximum of five camera TV ports in accordance with the customer s requests Adaptation possibilities are provided for heating
89. mination can be switched on or off as required Activation 1 2 deactivation is performed by pressing the FL X key for approx 1 second os Brightness adjustment of the LCD display is by 6 100X P h2 IF 2 FL pressing holding the FL key the toggle D A PIVIFIITIC 8 54 switch gt i x b The LCD display is also used for the 6 5 configuration of the microscope Set mode Fig 3 14 LCD Display Instead of the line scale for the lamp brightness the following can be displayed in the lower line 3 6 6 of the LCD display HAL off when the halogen illuminator is switched off 3200K when the lamp voltage has been set to the color temperature 10 5 V Description of the swung in filter set on the reflector turret e g DAPI FITC provided that the fluorescence shutter has been opened If no filter set is contained in the reflector turret the reflector turret position e g Refl 2 is displayed instead The currently set position appears in the LCD display for 4 seconds during performance of the following key functions Both lines of the display are used 1x Optovar display of the Optovar magnification factor 1 6x and the overall magnification Total M ag in the intermediate image LEFT RIGHT and Slider VIS Frontport Baseport display of the actual intensities at Sideport Frontport Baseport or VIS depending on the selected positions of both control elements e g SP
90. move FL reflector module 2 18 3 from the reflector turret e Use mounting device from the tool set to unscrew retainer ring 2 18 1 e Turn the reflector module around and allow the Fig 2 18 Changing the filter set in the FL filter 2 18 2 or 4 to drop on a soft surface teriector module e The barrier filter is inserted at 2 18 2 the excitation filter at 2 18 4 and both are secured using the retainer rings 2 18 1 2 7 4 Changing the beam splitter in the FL reflector module 1 Assembly of the filter and the beam splitter requires utmost care to prevent damage and contamination of the optical components We would recommend you to order completely equipped FL reflector modules since changing the beam splitter requires much skill Otherwise proceed as follows e Remove FL reflector module from the reflector turret Use screwdriver to loosen two slotted screws 2 19 1 Fig 2 19 Changing beam splitter 2 16 B 40 080 e 03 01 Axiovert 200 START UP Hold both halves of the reflector module together turn them around into the installation position and put them down Now tilt the upper module half 2 20 1 upwards and lift it out of the holding elements 2 20 5b of the lower module half Remove beam splitter 2 20 2 and spring frame 2 20 3 from the lower module half Remove old beam splitter and carefully place the new one on the spring frame 2 20 4 with the reflecting side p
91. n accordance with sections 2 12 2 through 2 12 4 by using the adjusting aid If required re adjustment must be performed depending on the operation time 3 3 5 3 Setting of epi fluorescence on the Axiovert 200 manual and Axiovert 200 M The first epi fluorescence setting is considerably facilitated if the Plan Neofluar objective 20x 0 50 and a specimen featuring pronounced fluorescence are used It is also possible to use demonstration specimens first e Switch on the HAL 100 e Swing in suitable objective e g Plan Neofluar 20x 0 50 via the nosepiece 3 24 4 e Move condenser turret to position H transmitted light brightfield or also phase contrast and then move to the specimen area to be examined e Use focusing drive for focusing e Keep light path in the reflected light part blocked at first using the fluorescence shutter by pressing the FL on off key 3 24 6 3 40 B40 080e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss Se SHS 460 4 1 103 fluorescence illuminator 2 Iris stop slider aperture diaphragm 3 Iris stop slider luminous field diaphragm 4 Nosepiece 5 Reflector turret 6 FLon off key Fig 3 24 Components for epi fluorescence on the Axiovert 200 B 40 080 e 03 01 3 41 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 Switch on the NHBO 103 fluorescence illuminator 3 24 1 via the power supply
92. ng e f required remove analyzer slider 0 2 3 or switch to free light path Turn setting wheel for Sideport right left vis 0 2 22 to position 100 96 vis visual Q e Turn setting knob for Frontport Baseport vis 0 2 23 to position 100 96 vis 1 9 Set beam splitting ratio to 100 vis 0 2 10 on the tube Switch off the Bertrand lens if available Move combined rotary slider knob 0 2 9 to position 100 96 vis Place a high contrast specimen on the microscope stage 0 2 21 Adjust the binocular component e Use the coarse fine focusing drive 0 2 6 to focus on the selected detail of the specimen Should no light be visible in the eyepieces switch on the halogen illuminator via the HAL on off switch 0 2 7 Use the toggle switch 0 2 8 to set the light 3 o intensity to comfortable brightness e Close luminous field diaphragm 0 2 16 until it A B c D E is visible in the field of view even if not in focus Fig 0 1 Diaphragm settings in transmitted 0 1 A light brightfield according to e Focus on the edge of the luminous field KOHLER diaphragm 0 1 B by moving the condenser 0 2 17 vertically e Center 0 1 C luminous field diaphragm via the centering screws 0 2 15 and 18 and open it until the edge of the diaphragm just disappears from the field of view 0 1 D e Remove one eyepiece from the eyepiece tube or swing in Bertrand lens and set aperture diaphrag
93. ng allocated to each magnification step Zeiss color code 0 20 numerical aperture HD reflected light brightfield and darkfield objective oo infinite tube length can be used without cover slip D 0 mm or with cover slip thickness D 0 17 mm or 0 can only be used without cover slip D 2 0 mm 0 17 can only be used with cover slip thickness D 0 17 mm and Oil oil immersion objective Ph 2 phase contrast objective with a green color ring and phase stop Ph 2 Color ring code for objective magnification Color ring on black brown red orange yellow green light dark white objective blue blue Magnification 1 25x 2 5x 4x 5x 6 3x 10x 16 20 40x 50x 63x 100x factor 25x 32x 150x The objective magnification e g 10x multiplied with the eyepiece magnification e g 10x and the Optovar magnification e g 1 6x results in the visual overall magnification example 10 x 10 x 1 6 160x The numerical aperture x 1000 e g 0 20 x 1000 200x is the highest useful magnification i e no further details are resolved above that limit In transmitted light applications the exact observance of the cover slip thickness of 0 17 mm is all the more necessary the higher the numeric aperture of the objective Therefore so called corr objectives can be set for different cover slip thicknesses via a correction ring For this a specimen area is searched and the position of the correction ring where optimum focus and image cont
94. nical stage 130x85 R L and mounting frame for mechanicalstade rare 2 10 2 5 2 Attachment of scanning 5 2 10 2 5 3 Attachment of specimen stage 250x230 object guide mounting frame for object quide IN seelisch 2 11 2 5 4 Attachment of heating stage sseeeeeeennnnn nennen nnne nnne nnns 2 12 2 5 5 Attachment of gliding Stage Zn an east 2 12 2 6 Attachment of Condensers u a Rilke 2 13 2 6 1 Condensers for the Axiovert 200 2 13 2 6 2 Condensers from the Axioplan 2 imaging Axioskop 2 product line 2 13 2 6 3 Changing the DIC prism in the condenser 2 14 2 7 cM 2 15 2 14 Attachment of reflector turrets u ee rete bte ae 2 15 2 7 2 Equipment on reflector Turret nee ae el ale lett 2 15 2 7 3 Changing the filter set in the FL reflector 2 16 2 7 4 Changing the beam splitter in the FL reflector 2 16 2 8 Gontiectornsto the lern tue ee Dee eis 2 18 2 9 Interfaces of the Axiovert 200 M sure 2 19 2 10 Switch microscope and 100 dc power supply on and 2 19 2 11 Equipo tential bonding terminal 2 19 2 12 HAL T100 halogerMlumiltidtQT ee i dete pe eee see a euere 2 20 2 12 1 Change attachment of the HAL 100 halogen
95. nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnennnnnn 3 11 luminous field diaphragm sh ansehen NU tee 3 42 M litis ila s A ac ie 4 4 Mieroscopesstage MEE EUER 2 11 3 11 mounting ee air an be ad een eed A HR ed Der a ERN KR ed LUPO Repos 2 11 N aul I ED 2 9 3 7 3 18 3 25 Object 2 11 OBJECTIVE fon tum a Fed 3 18 ella vii e 2 9 On JO SWIEGI oue 3 6 3 17 OPERATION u AE ME 3 3 Operation and function Controls eere ee 3 4 3 16 OPTOVARB teet E dt eret eus 3 20 Optovar aeiia user 3 7 A 6 B 40 080 e 03 01 ANNEX Axiovert 200 List of key words Carl Zeiss P DORIO COIL nee rS 3 25 performing Checks cus ieri nece caa en EEE 4 4 phase COD dsb eoi eti e i ibo idu te dedero ds fee e i ded 3 33 sjaro etos a 9 ro i 0 b en D 3 45 3 46 uc 3 11 R 3 40 REFLECTOR Tr 3 22 reflector ML C IPLE D CLE DIC PU Ea 2 15 3 13 3 20 S ej D 4 6 nr an M e EAD le N E 3 11 3
96. nsmitted light illumination e Switch on the microscope e Direct light beam against a projection area wall approx 3 m away Make sure not to look into the light exit opening of the illuminator Fig 2 26 Alignment of halogen illuminator e Use SW 3 ball headed screwdriver to set adjusting screw 2 26 3 in such a way that both images of the lamp coil on the projection area are defined as clearly as possible e Then set adjusting screws 2 26 4 and 5 in such a way that the lamp coils of one image exactly fill the gaps of the reflector image 2 26 1 2 12 3 Attachment of halogen illuminator e f required remove cover from the lamp mount 2 27 2 in the carrier for transmitted light illumination e Insert dovetail 2 27 2 of lamp housing 2 26 3 in carrier 2 27 2 and use SW 3 ball headed screwdriver to tighten clamping screw 2 27 1 e Connect 3 pin lamp plug 2 27 4 to 3 pin 12 V 100 W socket 2 27 5 on the instrument rear Fig 2 27 Attachment of halogen illuminator 40 080 03 01 2 21 START UP Carl Zeiss HAL 100 W halogen illuminator Axiovert 200 2 12 4 Fine alignment of halogen illuminator Fine alignment requires the diffusion disk to be removed e Loosen clamping screw of HAL 100 W 2 28 1 and remove the illuminator from the carrier for transmitted light illumination e Turn diffusion disk 2 28 2 out of the carrier manually anti clockwise
97. nstrument safety The Axiovert 200 Axiovert 200 M microscopes were designed produced and tested in compliance with DIN 61010 1 1010 1 Safety requirements for electrical measuring control and laboratory instruments and meet the requirements of appendix of directive 73 23 EC and the relevant CSA and UL directives The instruments meet the requirements of the EC directive 89 336 EC and the EMC legislation of November 9th 1992 This operation manual includes information and warnings which must be observed by the user The following warning and information symbols are used in this manual This symbol is warning which must observe under all circumstances 1 CAUTION This symbol is a warning which indicates a hazard to the instrument or instrument system CAUTION This symbol is a warning which indicates a hazard to the user of the instrument CAUTION Hot surface CAUTION UV radiation is emitted gt pb CAUTION Disconnect the instrument from the line before opening it 40 080 03 01 0 7 Carl Zeiss INTRODUCTION Notes on instrument safety Axiovert 200 The Axiovert 200 microscopes including original accessories may only be used for the microscope techniques described in this manual Particular attention must be paid to the following notes u AN gt E gt ED The manufacturer cannot assume any liability for any other applications possibly also involving individual
98. nual gy 31 okt EXD 34 33 32 Fig 3 1 Operation and function controls on the Axiovert 200 manual 3 4 B 40 080 e 03 01 OPERATION Axiovert 200 Axiovert 200 Key to Fig 3 1 On Off switch Nosepiece Compartment for analyzer slider can be equipped from the right or left Drive controls for XY movement of the mechanical stage Sideport right Setting wheel for Optovar turret max 3 positions Reflector turret Focusing drive coarse fine FL key on off for fluorescence shutter HAL on off switch Toggle switch for illumination intensity Frontport Binocular tube Binocular component of the tube Setting ring of eyepiece Eyepiece Polarizer D with 2 position filter changer Centering screw for condenser LCD display Adjusting lever of luminous field diaphragm Setting knob for vertical adjustment of condenser Centering screw for condenser Condenser Microscope stage HBO XBO adjusting aid Compartment for 3 position filter slider dia 25 mm Compartment for slider with iris stop as aperture diaphragm reflected light Compartment for slider with iris stop as luminous field diaphragm reflected light Sideport left Setting wheel for Sideport right Sideport left vis Clamping screw for Frontport Setting knob for Frontport Baseport vis Stop for focusing drive Baseport Key Set LM 40 080 03 01 Carl Zeiss 3 5 OPERATION
99. ode and return to the standard mode briefly press the Set LM key on the instrument rear The permanent storage of all the settings is confirmed by a beep Config amp Focus Settings Stored appears in the LCD display If no operation is performed in the Set mode for 60 seconds the microscope is automatically reset to the standard mode The settings made until then will not be stored If the instrument is switched off in the Set mode the settings performed will not be stored either 3 2 2 2 Setting of focus limit software limit In addition to the recognition of the hardware end positions the software also permits the setting of the focus limit i e the upper limit of the travel range of the focusing drive This limit is an absolute focus position which applies to all nosepiece positions and which is only corrected by the amount of parfocality alignment of the relevant objective In the factory alignment of the Axiovert 200 M the focus limit lies above the upper end position switch and is therefore not effective Activation of focus limits e Press Focus Up A key for more than 2 seconds The current position of the focusing drive is stored as software limit topmost Z position FOC Limit set appears in the LCD display for 4 seconds Shifting of focus limit e Keep the Work A key pressed and simultaneously move to the required position via the focusing drive The new focus limit is stored after the key is released 3
100. ointing downwards and then insert both parts together in the lower module half Make sure that the lateral catch of the spring frame 15 positioned in the relevant recess in the lower module half If there is no distance between a wooden pin and its mirror image when such a pin is carefully placed on the surface of the beam splitter this is the reflecting side of the beam splitter Reflector turret Carl Zeiss Fig 2 20 Changing the beam splitter Place upper module half 2 20 1 on the lower one 2 20 4 holding elements 2 20 5b and lugs 2 20 5a mesh with each other Hold both halves together and turn them around into the installation position Insert and tighten slotted screws Finally attach the adhesive label with the name of the filter combination to the side of the module 40 080 03 01 2 17 START UP Carl Zeiss Connection to the line Axiovert 200 2 8 Connection to line e Connect microscope socket 2 21 1 to the line socket via a line cable The microscope can be connected to line voltage between 90 and 264 VAC 50 60 Hz The microscope is equipped with a wide area power unit which automatically adapts to the available line voltage Voltage change is not required Fig 2 21 Axiovert 200 M rear The N HBO 103 illuminator for epi fluorescence is supplied via a separate power supply e The power supply 100 must be connec
101. on of the focusing drive on the microscope stand must not be changed 40 080 03 01 2 7 START UP Carl Zeiss Attachment of transmitted light illumination Axiovert 200 2 3 Attachment of transmitted light illumination 2 3 1 Attachment of carrier for trans mitted light illumination 100 W e Attach carrier 2 5 1 to the relevant contact surface on the rear of the stand and use the SW 4 Allen key to tighten the four enclosed hexagonal screws 2 5 2 e Connect plug 2 5 4 for the LCD display if available to the SB socket 2 5 3 at the instrument rear Alignment of the carrier 100 W is not required Fig 2 5 Attachment of carrier for trans mitted light illumination 100 W 2 3 2 Attachment of carrier for trans mitted light illumination 30 W 1 Before attachment of the carrier for transmitted light illumination 30 W 451380 0000 000 to the stand the control electronics included in the stand must be changed by Zeiss service staff Remove HAL 100 and N 103 illuminators from the microscope e f required remove carrier 100 W by loosening the four hexagonal screws SW 4 and disconnect plug of the LCD display from the SB socket Fig 2 6 Attachment of carrier for trans e Screw adapter plate 000000 1005 842 2 6 1 mitted light illumination 30 W onto the contact surface on the stand rear using the four hexagonal screws e Attach centering pin of the carrier f
102. or transmitted light illumination 30 W 2 6 2 to the adapter plate and tighten the three SW 4 hexagonal screws 2 6 3 Alignment of the carrier 30 W is not required e Connect power supply plug of the carrier for transmitted light illumination 30 W 2 6 5 to the 12 V 100 W connector 2 6 4 on the rear of the stand 2 8 B 40 080 e 03 01 START UP Axiovert 200 Screw in objectives Carl Zeiss 2 4 Screw in objectives Remove dust caps 2 7 1 from the respective openings in the nosepiece e Remove objectives 2 7 2 from the case and screw them in the nosepiece 2 7 3 starting with position 1 see engraved number in increasing order of magnification factors Make sure that the objective is correctly and securely locked Make sure to use dust protection caps on those nosepiece eyes which are not required Fig 2 7 Screw in objectives 40 080 03 01 2 9 START UP Carl Zeiss 7 H 4 N 4 y l 3 Fig 2 8 Attachment of mechanical stage 130x85 Fig 2 9 Insertion of mounting frame K 2 10 Attachment of microscope stages Axiovert 200 2 5 Attachment of microscope stages 2 5 1 Attachment of mechanical stage 130x85 R L and mounting frame for mechanical stage K The mechanical stage is mounted to the stand directly above three contact points with the relevant drilled holes To improve access during stage assembly the
103. oscope 40 080 03 01 2 5 Fig 2 2 Attachment of binocular tube Fig 2 3 Inserting the eyepieces START UP Carl Zeiss Attachment of binocular photo tube Axiovert 200 2 2 Attachment of binocular photo tube All the binocular tubes listed in the microscope configuration can be attached to the Axiovert 200 and Axiovert 200 M as described below Proceed as follows to attach a tube for the first time orto replace a tube Loosen hexagonal screw 2 2 2 using the SW3 ball headed screwdriver If a tube is to be replaced hold it and remove it in forward direction Remove the dust cap from the tube lens of the tube to be attached Insert dovetail of the binocular tube 2 2 1 into the stand mount 2 2 3 align it with the stand and tighten hexagonal screw 2 2 2 using the ball headed screw driver 2 2 1 Inserting the eyepieces and the centering telescope Remove both dust protection caps 2 3 1 and 4 from the binocular tube Remove both eyepieces 2 3 2 from the cases and insert them in the binocular tube until stop The centering telescope 2 3 3 which is used to view the aperture and phase stops and to center the phase stops can be inserted in one of the tubes instead of an eyepiece The variable eyelens permits focusing on these stops The eyepieces W PL 10x 23 Br foc can be attached to all tubes 2 6 B40 080e 03 01 START UP Axiovert 200 Attachment of binocular photo tu
104. osition and the Objectives in the nosepiece are shown in the lower LCD line e Activation of one of the two FOCUS keys permits the focus factor to be changed individually for the swung in objective Key FOCUS the drive moves faster Key FOCUS V the drive moves slower e Then focus on the specimen again and swing in the objective with the next lowest magnification While the nosepiece is being moved to the next position the focus position and focus speed values set for the previous objective are saved in the Set mode e Use the current objective to set the focus speed focus exactly on the specimen and move the nosepiece to the next position to store the settings e Repeat the above settings for all objectives The last settings should always be made with the oil immersion objectives e Finally the nosepiece must be turned by one further position to permit the values to be saved e To save the entire setting exit the Set mode by briefly pressing the Set LM key Resetting of Z focus factors and parfocality to default values e Activate Set mode e Press and hold FOCUS and FOCUS V keys simultaneously for approx 1 s until you hear beep 40 080 03 01 3 25 OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 Deactivation of Set mode and saving of settings The Set mode can be exited after conclusion of the settings The settings changes performed are automatically stored when the mode is exited To exit the Set m
105. pensation Use the coarse fine focusing drive 3 16 6 to focus on the selected detail of the specimen If no light is visible in the eyepieces check whether light exits the housing of the halogen illuminator If this is not the case switch on the halogen illuminator by pressing the HAL on off key 3 16 7 Use the toggle switch 3 16 8 to set the light intensity to a comfortable brightness Adjustment is made in small steps when the key is pressed half down pressure point and in big steps when the key is pressed down completely Close luminous field diaphragm 3 16 16 until it is visible in the field of view even if not in focus 3 17 A A B D E Focus on the edge of the luminous field diaphragm 3 16 B by moving the condenser Fig 3 17 Diaphragm settings for KOHLER 3 17 17 vertically illumination in transmitted light brightfield Center 3 16 C luminous field diaphragm using centering screws 3 17 15 and 18 and open it until the edge of the diaphragm just disappears from the field of view 3 17 D For aperture diaphragm setting remove one eyepiece from the eyepiece tube and set aperture diaphragm 3 16 20 to approx 2 3 of the diameter of the objective exit pupil 3 17 E Optimum contrast setting is dependent on the respective specimen Insert eyepiece again and if required refocus on the specimen via fine drive Match the light intensity via the toggle switch is The field size and the objective a
106. perture change after every objective change i e the luminous field diaphragm and aperture diaphragm settings must be repeated to ensure optimum results 40 080 03 01 3 31 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3 3 1 4 Transmitted light brightfield configuration of the Axiovert 200 M Each Axiovert 200 M microscope can be configured to permit the transmitted light brightfield technique 3 3 1 5 Transmitted light brightfield setting according to KOHLER on the Axiovert 200 M Put the Axiovert 200 M in operation as described in chapter 2 Configure the instrument in the Set mode Switch on the instrument and activate the Light M anager standard setting The illumination intensity the reflector turret setting brightfield position the condenser settings position of the turret and aperture diaphragm opening and the status transmitted light on or off are stored by pressing the Set LM key Motorized microscope components must be activated via the relevant control elements e Set K HLER illumination as described in section 3 3 1 for the Axiovert 200 manual e After the last setting item briefly press the Set LM key on the instrument rear to store the settings A brief beep is emitted after release of the key to confirm that the setting has been stored e The above diaphragm settings must be repeated for all objectives since the field size and the objective aperture are chang
107. piece with mounts for DIC 5 3 7 Bihoeular tube 43 12 3 5 d een rl utu aliua ds de lee usd 3 8 Binocular 452 23 ee 3 8 Setting of eyepiece distance on the binocular tube eeemnn 3 9 DISNEY Er er een Eee 3 9 LD condenser 0 55 6 position H Ph2 Ph3 1 2 3 10 LD condenser 0 35 6 position H PhO Ph1 Ph2 DIC 3 10 5 DOSIEION reflector turret 3 13 Iris Stop SIL eoe to ese tees entre S n Needs cach 3 13 Off switch and toggle SWIEGP es eh 3 14 Set LM mr CT engen heit 3 14 Operation and function controls on the Axiovert 200 M motorized 3 16 ER 3 19 Condenser 0 55 6 position H Ph1 Ph2 Ph3 DIC 3 20 Axiovert 200 e 3 30 Diaphragm settings for KOHLER illumination in transmitted light brightfield 3 31 Centering the phase stop on the 4 eee 3 34 Centering of phase stop bright in condenser with phase ring dark in objective 3 34 Components for the transmitted light DIC technique on the Axiovert 200 3 37 Settind or VAREL EONERASE doe peer tel egeta se mecha an Eee gera cupio 3 38 VAREL contrast with microtiter plates
108. r out of the 0 position and to set the optimum contrast in this way Since the DIC technique uses polarized light it is impaired if birefringent components foils sometimes used with histological sections are positioned between polarizer and analyzer The same applies to Plexiglas culture chambers if the chamber bottom is made of plastic In such cases it is recommended to use chambers with glass bottoms to avoid the loss of optical performance 3 36 B40 080e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss 1 Polarizer D fixed optional rotary 2 Condenser 3 DIC slider 4 Analyzer slider fixed 5 Analyzer slider 30 Fig 3 20 Components forthe transmitted light DIC technique on the Axiovert 200 B 40 080 e 03 01 3 37 OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3 3 4 Setting of VAREL contrast in transmitted light 3 3 4 1 General principle VAREL contrast provides a relief like image of objects and can be used as an alternative to phase contrast VAREL contrast can also be used on curved surfaces e g 96 well microtiter plates for which no contrast can be achieved with the phase contrast technique no flush ring positioning 3 3 4 2 Configuration of the Axiovert 200 manual and Axiovert 200 M Condenser with turret equipped with VAREL stops Objectives which are suitable for VAREL contrast e g A Plan Ph1Varl or L
109. rast are obtained is determined refocusing is always required Immersion objectives are always insensitive to differences in cover slip thickness When immersion objectives are used the air between the cover slip and the objective is replaced with a liquid which is immersion oil in most cases 1 10 B 40 080 03 01 Axiovert 200 INSTRUMENT DESCRIPTION Objectives Carl Zeiss The following objectives are available for the Axiovert 200 Axiovert 200 M microscope Objective type Magnification numeric aperture Cover slip thickness D in mm Free working distance A in mm Contrasting A Plan A Plan A Plan LD A Plan LD A Plan LD A Plan LD A Plan LD A Plan LD A Plan Cover slip cap LD Achroplan Achroplan Achroplan LD Achroplan LD Achroplan LD Achroplan LD Achroplan LD Achroplan Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Neofluar Plan Apochromat Plan Apochromat areferstod 1 40 080 03 01 4x 0 10 10x 0 25 20x 0 40 20x 0 40 40x 0 60 40x 0 60 63 0 75 5 0 15 5 0 15 10 0 30 10 0 30 16 0 50 Imm 16 0 50 Imm 20x 0 50 20x 0 50 40x 0 75 40x 0 75 40x 1 30 Oil 40x 1 30 Oil 63x 0 95 Korr 100x 1 30 Oil 100x 1 30 Oil 63 1 40 Oil D 0 6 D 0 6 D 0 6 D 0
110. rrier for 30W Illuminator N HBO 103 000000 1007 980 Collector N HBO 103 XBO 75 000000 1007 976 Achromatic collector 000000 1007 978 Mercury vapor short arc lamp HBO 103 W 2 380301 9350 000 Variable intensity lamp control AttoArc 2 N 000000 1007 975 Power sup unit for N 75 90 250V 50 60Hz 130 VA 000000 1003 924 Illuminator N XBO 75 000000 1007 981 Collector N HBO 103 XBO 75 000000 1007 976 Achromatic collector 000000 1007 978 Xenon lamp XBO 75 W 2 380053 9870 000 Protective gloves 417008 0000 000 Face protection shield 417009 0000 000 Lamp housing HBO 50 with socket 447220 0000 000 Lamp collektor HBO 50 SF 25 447270 0000 000 HBO 50 super pressure mercury lamp 381619 0000 000 Power supply unit for HBO 50 100 240 V 000000 1113 833 Microscope configurations and modules sheet 3 B40 080e 03 01 INSTRUMENT DESCRIPTION Axiovert 200 Microscope configurations and modules Carl Zeiss 2 axes control panel 457433 0000 000 HRZ 200 fine focusing stage 000000 1013 186 only in combination with LSM 510 Dust cover 459330 0000 000 Motor control MCU 28 457428 0000 000 CAN bus cable 2 5 m 457411 9011 000 Scanning stage 120x100 000000 1113 509 Path deflecting mirror mot Subbus 000000 1095 490 LD condenser 0 55 mot H Ph 1 Ph 2 Ph 3 DIC DIC 000000 1005 848 Excitation filter wheel 8 positions MOT for 25 mm filters 000000 1008 10
111. rture diaphragm always fully opens automatically NA 0 55 The turret position appears in the lower line of the LCD display e g Cond DICII Cond NA 0 55 is displayed in a phase stop position and the key pair A is without function Fig 3 15 Condenser 0 55 6 position H Ph1 Ph2 Ph3 DIC DIC The motorized condenser can only be used with the motorized stand or with a manual stand which is equipped with an interface PC board 1x OPTOVAR key for the motorized change of Optovar settings 3 13 12 Key for the selection of the Optovar magnification switching sequence 1x 1 6x 2 5x Key pressed briefly current position is shown in the LCD display for 4 s or until the next press of the key without LCD display the position is approached imediately key pressed again target position is displayed and approached n the LCD display the selected Optovar magnification and the overall magnification are shown 3 20 B40 080e 03 01 OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss LEFT RIGHT key for the motorized switching of the Sideport turret 3 13 13 Key to switch the beam splitting ratio for Sideport doc right Sideport doc left and VIS visual observation through the binocular Five motorized configurations are possible LSM connector SP L and Sideport 60 R LSM connector BP and Sideport 60 L LSM connector BP and FCS connector SPL Safety device LSM PASCAL Change to beam path mot
112. s on the mat 1 to 6 correspond to the objective mounts on the nosepiece 1 to 6 The objective table below specifies the hole size for the most common objectives that must be cut out for the corresponding objective e Put cover mat onto nosepiece IS Press down the cover mat only as far as is possible without noticeably slowing down the motion of the nosepiece e Next put a silicone ring 2 38 3 over every objective There are two types of silicone rings available Size 1 small Size 2 large The objective table also specifies the size of the silicone ring to be used for the most common objectives On objectives with correction ring the silicone ring must be above correction ring On all other objectives slide the silicone ring up to about 3 mm before the beginning of the thicker part of the objective to ensure correct function of the objective s specimen protection mechanism e Then slip the cut drain hose over the two drain connectors 2 38 6 Put the other end of the hose into the collecting tank The liquid can only drain if the collecting tank provides venting This is made possible e g by a hole in the lid or by screwing the lid only loosely 2 28 B 40 080 e 03 01 START UP Axiovert 200 Attachment of Aqua Stop Carl Zeiss You can fix the drain hose using the provided clips Ks Fix the hose in such a way that the drain gutter is not bent in focusing e Attach the microscope stage again
113. stion mark appears in the upper line of the LCD display instead of the objective name Fig 3 11 Off switch and toggle switch activate the Set mode keep the Set LM key 3 12 1 on the instrument rear pressed for approx 1 second until a double is Sy emitted The objective name in the LCD display starts blinking e Now set the magnification and contrasting 1 technique one after the other for each nosepiece position by using the toggle switch 3 11 2 on the instrument front to quickly or slowly scroll through a list of all possible combinations in forward or backward direction Fig 3 12 Key Set LM To exit the Set mode and return to the standard mode briefly press the Set LM key on the instrument rear The permanent storage of all the settings is confirmed by a beep LightM anager Settings stored appears in the LCD display D If no adjustment is performed for more than 60 seconds the Set mode is exited automatically 3 14 B40 080e 03 01 OPERATION Axiovert 200 Axiovert 200 Carl Zeiss 3 1 2 2 Light Manager The Light M anager is automatically activated each time the Axiovert 200 is switched on The Light Manager automatically sets the illumination intensity of the halogen illuminator last saved for each individual objective position Therefore the correct brightness is immediately available in almost all cases During the objective change the lamp voltage is reduced a
114. ted to the line via the line socket 2 22 1 also see section 2 13 3 Attachment of illuminator N 103 The ebq 100 dc is equipped with a wide area power unit which automatically adapts to the available line voltage Voltage change is not required Fig 2 22 Powersupply 100 dc rear 2 18 B 40 080 e 03 01 START UP Axiovert 200 Interfaces Microscope and power supply Carl Zeiss 2 9 Interfaces of the Axiovert 200 M 1 Prior to connecting any components switch off the microscope The Axiovert 200 M is connected to a PC via RS 232 interface 2 23 2 Motorized components of the Axiovert 200 M e g the motorized condenser are connected via the SB interfaces 2 23 3 Further external control components e g the 3 axis motor control MCU 28 of the scanning stage must be connected to the CAN SB connectors 2 23 1 2 10 Switch microscope and ebq 100 dc power supply on and off Switch the microscope on and off using the line switch 2 24 1 e If a fluorescence illuminator e g N 103 is connected see section 2 13 3 Attachment of illuminator N HBO 103 switch power supply ebq 100 dc on and off via the line switch 2 24 2 2 11 Equipotential bonding terminals Terminals for equipotentil bonding for electrophysiological measurements are located on the rear side of the stand 2 23 4 and on the underside of the binocular tubes 000000 1005 827 and 000000 1005 828
115. ual shutter 19 Turret of condenser 3 Compartment for slider 11 Binocular component of the tube 20 Setting wheel for aperture stop analyzer 12 Setting ring of eyepiece on the condenser 4 Setting wheel for Optovar turret 13 Eyepiece 21 Microscope stage 5 Reflector turret 14 Polarizer D with 2 position filter 22 Compartment for aperture stop 6 Focusing drive coarse fine changer slider 7 HAL on off key 15 Centering screw for condenser 23 Setting wheel for Sideport 8 Toggle switch for illumination 16 Adjusting lever of luminous field 24 Setting knob for intensity diaphragm Frontport Baseport 9 Rotary or slider knob for 17 Setting knob for vertical vis doc beam splitting adjustment of the condenser Fig 3 16 Axiovert 200 3 30 B40 080e 03 01 OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss Swing out 3 position filter changer 3 16 14 Place a high contrast specimen on the microscope stage 3 16 21 Match the eyepiece distance interpupillary distance to the user s individual interpupillary distance For this purpose pull apart or push together the binocular component 3 16 11 of the tube Set the zero point of ametropia compensation on the setting ring 3 16 12 of the eyepieces 3 16 13 to the white dot if no eyepiece reticle is available to the red dot if eyepiece reticle is available If required optimize focus of the selected object detail via the setting ring of the relevant eyepiece for ametropia com
116. using a spacer bar and a spacer disk Use the two shorter hexagonal screws to screw the spacer bar 2 10 5 to the two front contact points Place spacer disk 2 10 3 on the rear contact point Place specimen stage 2 10 2 on the stand and first fix it to the rear contact point from above using the longer hexagonal screw 2 10 1 Make sure that the screw goes through the drilled hole of the spacer disk Then screw specimen stage to the right and left of the spacer bar from below using two hexagonal screws 2 10 4 Also tighten the rear screw 2 10 1 Attach object guide 2 11 1 to the specimen stage from the right or left and fix it in position from below using three hexagonal screws 2 11 2 Then push mounting frame for object guide 2 11 3 under the two springs of the object guide from below until it clicks into position 40 080 03 01 Attachment of microscope stages Carl Zeiss Fig 2 10 Attachment of specimen stage 250x230 Fig 2 11 Attachment of object guide and mounting frame 2 11 START UP Carl Zeiss Attachment of microscope stages Axiovert 200 2 5 4 Attachment of heating stage The heating stage is attached to the contact points of the stand using three spacer disks e Remove any available microscope stage and additional mounting components e Place spacer disks 2 12 2 on the three contact points of the stand e Place the heating stage 2 12 1 on t
117. ut pushing in the cylinder until stop Compartment for 3 position filter slider 3 1 26 for 3 position dia 25 mm filter slider Push in filter slider until the required stop position with the labeling being visible from the front Compartment for iris stop slider reflected light 3 1 27 and 28 Accepts the slider with iris stop 3 10 1 as aperture or luminous field diaphragm for the setting of KOHLER illumination Push slider into the compartment until stop Symbol for stop opening wedge points to the user Use lever 3 10 4 of the slider to open or close the iris stop lower position The two centering screws 3 10 2 and 3 socket head SW 3 permit the stop to be centered in the beam path 40 080 03 01 Fig 3 10 Iris stop slider 3 13 OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3 1 2 Switching on and basic settings on the Axiovert 200 manual Switch on the microscope with the on off switch on the right side 3 11 1 The indicator light in the switch lights up The microscope is ready for operation after a few seconds 3 1 2 1 Configuration Set mode If the microscope is equipped with the LCD 1 display the correct objective names must be assigned to the objective positions first after the microscope has been switched on or when objectives have been changed The objective positions are entered in the Set mode In the case of the first entry a que
118. utomatically to avoid glare and to permit the required brightness to be achieved sooner FL key on off for fluorescence shutter If this key is pressed briefly the optional fluorescence shutter reflected light can be opened or closed Opening is confirmed by a beep The Light Manager remembers the setting of the halogen illuminator separately for the opened and closed shutter and resets it automatically after a changeover i e the correct lamp brightness is always available immediately after any change between reflected and transmitted light D This function of the fluorescence shutter key is also beneficial for pure transmitted light operation to change quickly and easily between two different settings of the lamp brightness e g in the following cases change between the brightfield and darkfield contrasting technique change between differently absorbing filters in the beam path change between visual observation and documentation using a camera Programming of switching status The individual settings of the Light Manager can be saved permanently so that they are always available immediately when the instrument is switched on To save the Light Manager parameters briefly press the Set LM key on the instrument rear Saving as switching status is confirmed by a beep LightManager Settings stored appears in the LCD display 40 080 03 01 3 15 OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3 2 Axiovert 200 M

Operating Manual Axiovert 200 / Axiovert 200 M Inverted Microscopes

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