Wrinkle Analyzer TiVi90 User Manual
Contents
1. Expem Thie Pis Parken Semen Manual Aboa Carne Acts Wrinkle Analyzer TiVi90 User Manual User Manual 3 3 Version 3 3 October 2013 PIONEERS IN TISSUE VIABILITY IMAGING TIVi90 Wrinkle Analyzer Dear Valued Customer Welcome to the WheelsBridge Wrinkle Analyzer TiVi90 system intended for automatic analysis of wrinkle appearance The Wrinkle Analyzer TiVi90 was designed especially for observer independent analysis of wrinkles in association with evaluation of skin care products and follow up of treatment regimes The Wrinkle Analyzer TiVi90 utilizes a highly sensitive digital camera equipped with polarization filters making it possible to suppress cross polarization mode or enhance co polarization mode surface reflections from the skin surface The versatile system software based on the MATLAB high performance language for technical computing allows for rapid and easy capturing and analysis of images Among the many useful features of the Wrinkle Analyzer TiVi90 software the following are of particular interest Automatic capturing of photos Automatic tracking of wrinkles Display of winkle profile 3D display of wrinkle structure Quantitative measures of wrinkle depth volume and asymmetry appearance Simultaneous analysis of up to 10 wrinkles Trend analysis window to display changes in wrinkle appearance over time Creation of wrinkle maps Possibility to load and save entire sess2. S ju c d Vinnkke Hurnber paac Display Load Sassiens Export a Dapth C valuma Agyrnm Gassian Chota 28 Again it can be clearly seen that the Depth of each wrinkle increases with session number and increasing forehead frowning 29 Click the Volume radio button to display the corresponding Volume values Trend iia x V alurmg wersus Venkle Hurmbar Volume value 2 Winrkla Humber Select Display Lost Sessions Export C Depth Ce Volume CO Agumm Sagcion ME 30 Click the Asymmetry radio button to display the corresponding Asymmetry values Wrinkle Analyzer TiVi90 3 3 Sida 17 TIVi90 Wrinkle Analyzer Asyrii remus Wnnkia Humber 50 as E E E ams E 2 V nnkila Hornber ms epeci Disphry load Seseicns Export pih O valume a Beyrmm 31 Click the Export button menu and navigate to a folder into which the Trend data is to be stored in MS Excel ASCII format Insert a File Name in the File Name edit box in the Save As window and click the Save button 32 Click the Close button the close the Trend window 33 Navigate to the folder in which the MS Excel spread sheet was saved and double click its name If MS Excel is installed the spread sheet will now open This spread sheet contains numerical information about the Wrinkle characteristics that can be used for further analysis A further example demonstrates the possibility
3. Pick a file Look in O TVBUdemonetraton IF Acess Th B sessnn Documarts Ceaskinp Wy Computer File name Asa T EXISagsIOn My Netwark Files nfhypn 25 Several sessions can be loaded simultaneously If all sessions are to be loaded point at the first session Asess TiVi9O0session with the mouse Press the shift button on the key board while dragging the mouse to point at the last session Fsess_TiVi90session All files are now high lighted Click Open to upload the sessions Alternatively separate files can be selected by clicking the actual file names while pressing the Ctr button on the key board The Trend window should now look like Depth werzus Session a Der Tea A em weht H Semn Tag THOS M35 Beag 25 Cea ag TOO Daesm ItuAnQ IDCS 19 00 B og OS 1 Peas Aad 51 Semon Hame amp Recoding Daie Salari Dicey 26 The diagram displays the Depth of the selected wrinkles black wrinkle No 1 brown wrinkle No 2 and orange wrinkle No 3 for the different sessions The name and dates at which the original photos were captured are displayed along the x Wrinkle Analyzer TiVi90 3 3 Sida 16 TiVi90 Wrinkle Analyzer axes for the actual sessions For all wrinkles analyzed the trend is towards a greater depth with increased frowning 27 Click the Wrinkle button to display the Depth values versus wrinkle number along the x axes Trend Depth wersie ionkla Humber ab JE 3b Ba us 3
4. Quake Peel Sie Track Lengi Track Beith Trmck Limi trop indes Wi gut of Tracic Degin bigis 2 Draw a ROI around the areas with craw foot wrinkles Wrinkle Analyzer TiVi90 3 3 Sida 36 TiVi90 Wrinkle Analyzer yot Boesmebs Sets Droplets Manuel bout Dera Asostrat Wet Tit Heght Me Wrinkle Appearance Cici Bio Frc Lais irar 010023273 Phan Parameters Wrinkle Paraiwaeis Cris Faot 0084 Track Foii Sira Guids Frit Som Tract Langit He aud et Track Depi jode 3 Click the Show ROI button to display the ROI area Panmetar Settings DecgDaim Mlama About Dero Anbei ECL Page Photo who B8 Het 1125 Virinkbe Appearance Click ca a ermi be siart tracing Darin 1T mmr 2012 18 4 0 252 Track Dei Sine Guida Poni Sin Tract Langh Track eii Traci Liri Slog kiez fic aui of Treck Dupth Mods 4 Click the Wrinkle Map button to open the Wrinkle Map window Wrinkle Analyzer TiVi90 3 3 Sida 37 TiVi90 Wrinkle Analyzer Wrinkle hap Scan Through Scan Through NENNEN A Wirnide Three hod EE OB A 5 In the Wrinkle Map window a set of wrinkles with a depth determined by the threshold setting in the Wrinkle Map panel is displayed 6 Print 137 in the Wrinkle Threshold edit box or adjust the setting by use of the Wrinkle Threshold slider to change the threshold Wrinkle Analyzer TiVi90 3 3 Sida 3
5. a volume of 7 7 and an asymmetry of 2 The length of the entire track with the actual setting is 100 pixels The width within which the tracking is performed is 1O pixels counted up and down from the track line as indicated by the green guide point lines Wrinkle Analyzer TiVi90 3 3 Sida 10 TiVi90 Wrinkle Analyzer natu MEME MEM MEM MEE Winkie Appearance Trackn Corspletg Traci Lage 103 r TERI Wt Dept iE yore 127 A ta Fi gat ol Truck lez uada 10 Place the mouse pointer to the left of the first track on the same wrinkle and click the left mouse button The system now tracks the second wrinkle section in this photo The window should look like Wrinkle Appearance mai ea rh Wwoeewe T5 Ga d p URDU cA ea E wt Wrinkle Analyzer TiVi90 3 3 Sida 11 TiVi90 Wrinkle Analyzer 11 Place the mouse pointer on the wrinkle in the lower right section of the photo The system now tracks the third winkle segment in this photo The window should look like Espa Ti Pre Procemer Panameter bngs Marsi dost ena Miami Wrinkie ppearanpce Wil Des 1EZ Vs E Gum OT Track Pet Gare Gente Poni Sz Tract Leni Track Pyme Traci Leni Srp ria bia Cash ed Treci Decor Elda 12 Click the Step Through button in the Wrinkle Parameters panel three times to successively display the average profiles of the three tracks The associated WAISs Depth Volume and Ass are displayed in the
6. 5 Click Display Sessionto display the results in a separate window 6 Click the Clear All button and load the photo forehead 0005 7 Click the Load Coord button and navigate to the folder in which the coordinates for the tracks in forehead 0004 were saved Double click MyCoord_TiVi90tracks mat The main window should now look like Larn TAi freA me mem Banera hrg Manual Abozi Caro Aroriari we hari Hit E Wrinkles Sppeannce as 54hag c UE ie cs 8 The three green dots represent the centre of the middle of the individual tracks in the photo forehead 0004 They should be in the vicinity of the corresponding wrinkles in the photo forehead 0005 now displayed 9 To move the green dots closer to the respective wrinkle point on a green dot press the left mouse button and drag the dot to the final position where the mouse button is released Repeat this procedure for all three green dots The main window should now look like Wrinkle Analyzer TiVi90 3 3 Sida 25 TIVi90 Wrinkle Analyzer byst T iTnm Procemmzr Pamaen barua Abcub Carne Amiant 10 To make the final adjustment of the green dot positions click the Adjust Coord button 11 Click Start Tracking to produce the tracks in the photo displayed The main window should now look like Lor TBnm Procamuer Pamen athinzz Barus About Dema Amostar t 12 Click Display Session to display the results in
7. a separate window Wrinkle Analyzer TiVi90 3 3 Sida 26 TIVi90 Wrinkle Analyzer 13 The wrnkle profiles in the photos forehead 0004 and forehead 0005 can now be compared This concludes the GETTING STARTED section Wrinkle Analyzer TiVi90 3 3 Sida 27 TiVi90 Wrinkle Analyzer 4 DROPDATA Using DropData is a convenient way of bringing image wrinkle data such as Depth Volume and Asymmetry to a table or Excel spread sheet Assume that a wrinkle in a photo has been tracked and the 77Vi90 Wrinkle Analyzer window looks like Espot PurameterSetengs OCropOetse Manual About Darmo Aaarmtart P ot rape Phat With a Height TED Wirind be A pepe eran ce Treng Gone Track erar E Dmm TTamar 2812 tie Wor epi dT Velume 6 4 gy l A Track Point ire Ginie Pont Erre Frisch Lenglh Trick Fd Trek Lirwit inp indes Fia put cF Track Daph za 1 Select DropData in the pull down menu to open the DropDataSheet Wrinkle Analyzer TiVi90 3 3 Sida 28 TiVi90 Wrinkle Analyzer Table Hame __ No Length Width Mode Depth Vol Ass Comment n i m ii 1 2 Click the Export to Dropdata button in the 7iVi Wrinkle Analyzer window The wrinkle data now appears in the first raw in the DropData window Wrinkle Analyzer TiVi90 3 3 Sida 29 TiVi90 Wrinkle Analyzer Ne Length Width Mode Depth Wot Ass Comment 5il 11 71 4 A Tap FOOD S 4000 Controls ceci Photo Lomd Tabie Export
8. interest try to select the region of interest to be as similar to that one in the first photo as possible and click the Show ROI button 21 Use the green dots in the centre of the tracks in the Session Display forehead 0001 window as reference points and other landmarks in this photo to place the mouse pointers in the same positions on the wrinkles in the forehead 0002 photo in the same order as in the first photo After having generated all three tracks the main window should look like Wrinkle Analyzer TiVi90 3 3 Sida 14 TiVi90 Wrinkle Analyzer Epon THiPre Procemser frimer sainz Manual Abest Dine Askism Fiona Tring Compe Track Leng 10 Track Poet fane Dure Pom zz Track Lenge Track PETS Track Land Sop nda Ho qur ot Traci Drecrt bibs 22 Click the Save Session button and save this session under name Bsess Continue this procedure with the remaining photos forehead 003 forehead 006 and save the corresponding sessions under the names Csess Dsess Esess and Fsess in the TiVi90demonstration folder In case a mouse point is misplaced the corresponding track can be deleted by clicking the Undo Track button 23 Click Display Trend to open the Trend window m Depth varaia Session m seinrt Displsy m Eisen ROS Wrinkle Analyzer TiVi90 3 3 Sida 15 TiVi90 Wrinkle Analyzer 24 Click the Load Session button to open the Pick a File window and load sessions from the disk
9. of Depth Volume and Asymmetry of the wrinkles versus wrinkle number or session Nw nd ila r Ima ee BON Denia B Pe ag Us Lig 25 4 23 IE Eras 3g 2008 SS Denman ame A Hedat Cale Suact Diep us 8 Depth Volurs Apre nkdg iit Wrinkle Analyzer TiVi90 3 3 Sida 48 TIVi90 Wrinkle Analyzer 1 Load session to upload one or more sessions simultaneously To load more than one session press the Ctr button and click on the sessions to be uploaded by the mouse pointer in the Pick a File window 2 Export to export the results to a MS Excel spread sheet for further analysis 3 Wrinkle to select if the Wrinkle Appearance Parameters are to be displayed as a function of wrinkle number or date of recording the photo associated with the actual session Session to select if the Wrinkle Appearance Parameters are to be displayed as a function of session number 4 Depth in Select Display frame to display the depths of the wrinkles 5 Volume in Select Display frame to display the volumes of the wrinkles 6 Asymmetry in Select Display frame to display the asymmetry of the wrinkles 6 5 DropData 1 Open the DropData window by selecting DropData from the TiVi Wrinkle Analyzer pull down menu Wrinkle Analyzer TiVi90 3 3 Sida 49 TIVi90 Wrinkle Analyzer do Length Width Mode Qepth Vol amp s Comment L gris A R OE 1 i th Ton 44 700 Sanii LanaWE 0DO6 L Len W
10. score 13 Reset to reset the Scan Through procedure and display all wrinkle segments 14 Close to close the Wrinkle Map window Wrinkle Analyzer TiVi90 3 3 Sida 53
11. to analyze also the minutest wrinkles on the back of the hand It is assumed that the photos are captured with the polarization filters set to co polarization mode 1 Open the TiVi90 Wrinkle Analyzer main menu 2 Click the Load Photo button and select the Hand 0001 file in the TiVi90demonstration folder The main window should now look like Wrinkle Analyzer TiVi90 3 3 Sida 18 TiVi90 Wrinkle Analyzer besmi d nn Pe brean re arum Oe Abosi Darme Bain lt a Mid Her 22M Day oT mi a ER Ev El 3 Open the Parameter Settings window and set the parameters in accordance with the figure below bpm TW ePerPeoeger Peehi hj Menuet brub Uere Aimar i ud ue Tapt Caicamdire ie op UT 4 Observe that the Track Length has been set to 50 and the Track Width to 20 Click the OK button t accept the parameters and close the Parameter Settings window 5 Draw a ROI over the base of a finger Wrinkle Analyzer TiVi90 3 3 Sida 19 TIVi90 Wrinkle Analyzer Fain WW qm Hw INE Dads Th dag PE Bhi ipeo Tiperi Pir e ieee Abot Dero Anm Track Pear Sura ae arj Ton Traci Lagi iara vit Fraga Lane Le Ed B cupo Trine eei Mera WU 38 1 2 x fil bb 7 We will start by analyzing the horizontal major wrinkles and then continue with rotating the photo to analyze the minor wrinkles Produce tracks by placing the mou
12. 40 51 WAL Daphh 2i 2 Wobma 105 Ame 25 8 Click the Export to DropData button to export the wrinkles data from this new photo to the DropData table which now should look like Wrinkle Analyzer TiVi90 3 3 Sida 33 TiVi90 Wrinkle Analyzer Table Hame Depth Vol Ass LenaWR 0006 1 2 Tor 11 7000 5 4000 3 1 0 LenaWR 0006 1 50 0 Top 11 7000 5 4000 LenaWR 0006 2 il 14 6000 8 2000 0 0 LenaWR 0007 1 24 2000 40 5000 Select Photo LenaWR D006 Delete Photo LenaWR 0006 9 Click Bar Graph to display the data in a Bar Graph diagram Wrinkle Analyzer TiVi90 3 3 Sida 34 TIVi90 Wrinkle Analyzer pe Lenat 0006 2 Lena ARD006 Lena AR 0006 1 UU Number of Columns Number of Rows 10 Click the Export Data button in the DropData window to export the wrinkle data to an Excel spread sheet 11 Click the Close button to close the DropData window Wrinkle Analyzer TiVi90 3 3 Sida 35 TiVi90 Wrinkle Analyzer 5 WRINKLE MAP The Wrinkle Map is particularly useful for displaying the appearance of a group of adjacent wrinkles such as craw foot wrinkles appearing close to the eye 1 Click Load Photo and upload the CrawFoot 001 photo from the TiVi90demonstration folder Export ParereterSettiing Droplets Mimas About Dern Assistant uat Pee Phicta Ww Ei H eB Wrinkle Appearance Draw a Regen et niere De Taa aa Pho Farameiurs Virinkla Pie bore Cae aH E Tr i Prit Eze i
13. 8 TiVi90 Wrinkle Analyzer e Wrinkile Map Scan Through 7 Drag the Remove Small Objects slider to the right to remove small wrinkle segments from the Wrinkle Map Scan Through Scan Through Wrinkle Analyzer TiVi90 3 3 Sida 39 TiVi90 Wrinkle Analyzer 8 Click the Enumerate button to assign and display numbers to the individual wrinkle segments scan Through Scan Through lengva Small Coeds 9 Click the Scan Through button to display the wrinkle segments one by one The parameters of the actual wrinkle segment will now be displayed in the Scan Though panel Wrinkle Analyzer TiVi90 3 3 Sida 40 TiVi90 Wrinkle Analyzer Wrinkle Map Scan Throwgh Scan Through Sean Theeugh 10 Click the Reset button To remove segment 2 which is part of the eye brow rather than a wrinkle segment from the Wrinkle Map point at this segment by the mouse pointer press the left mouse button and drag the mouse pointer to a position outside the Wrinkle Map with the mouse button pressed Wrinkle Analyzer TiVi90 3 3 Sida 41 TiVi90 Wrinkle Analyzer Wrinkle Map Scan Through Scan Through 11 The remaining wrinkl
14. 8 Language to set the actual language available only in stand alone mode 29 About to display the Wrinkle Analyzer TiVi90 About window 30 DemoAssistant to open the Demo Assistant for online assistance on how to use the Wrinkle Analyzer 6 2 TiVi 90 Wrinkle Analyzer Parameter Settings window The Settings Parameter window is used for setting and saving the different track parameters Parameter settings Track Length and Width Siac Depih Calculaiian kode Track Length I Track Width A21 Track aid Guide Pons Eira Track parmi iu Kd ep pint e Tap l Tire k ri ali es WY Track Limit zip indas IPC i Humber Dur OF Track Endpainis 1 Factory Settings used for uploading the factory values Default values of the track parameters 2 Save Settings used for saving the actual track parameters These values are automatically uploaded the next time the Wrinkle Analyzer TiVi90 is opened Wrinkle Analyzer TiVi90 3 3 Sida 46 TIVi90 Wrinkle Analyzer 3 Load Settings used for uploading the saved track parameters 4 OK click this button to accept the new settings and close the Parameter Settings window The following tracking parameters can be set either by dragging the slider to the target value or by printing the target value in the edit box and clicking the keyboard Enter button 1 Track Len
15. Deis LangVyR DoOa Deleig Photo Seve As Save Lana A 0006 3 Track a new wrinkle and again click the Export to DropData button New wrinkle information is now added to the DropDataSheet table Wrinkle Analyzer TiVi90 3 3 Sida 30 TiVi90 Wrinkle Analyzer Table Hame Width Mode Depth Vol Ass Comment i 20 Top 117000 54000 3 0 0 0 0 20 Top 11 7000 5 4000 LenaVWR 0006 2 l 20 Top 14 6000 8 2000 55 Controls Select Photo Load Table Export Data LenaWR 0008 Delete Photo Save LenalVR 0006 4 Upload a new photo in the TiVi Wrinkle Analyzer window 5 In order to place the wrinkle center coordinates at the same position in this photo select the first LenaWR 0006 photo from the Select Photo menu in the DropData window to open the Show Photo window Wrinkle Analyzer TiVi90 3 3 Sida 31 TiVi90 Wrinkle Analyzer Hame LenaWR 0006 6 Click the Tracks radio button to display this photo with tracks drawn Hame LenaVR 0006 m e 7 This window with center coordinates of wrinkles in the photo LenaWR 0006 can now be used as a guide to set the tracker coordinates in the actual photo displayed in the TiVi Wrinkle Analyzer window Wrinkle Analyzer TiVi90 3 3 Sida 32 TiVi90 Wrinkle Analyzer E n TEL Expert Parerrweter Setting DropData Manuel About Demo Gszetant Photo Ven idio Hegu HR Wrinkle Appearance Trasckng Cornphir Track Length 51 Cute Teer 62
16. R 0006 2 2 i 21 2 324 EXWR 1208 Controls Soles Dhato eer Expori Dele Lena AF D TIA a Dete Preto Save os mn Lana A 0808 2 The table displays wrinkle data such as wrinkle number track length track width track mode wrinkle depth wrinkle volume wrinkle asymmetry and a column for inserting comments in the table 3 The Name of the table selected by the Save As function is displayed above the table 4 Load Table to load an existing DropData table 5 SaveAs to save the actual table under a selected name 6 Save to save the actual table under its preselected name 7 Export Data to export table data to an Excel spread sheet 8 Select Photo menu to select a photo to display in the Show Photo window 9 Delete Photo to delete a photo from the actual table 10 Bar Graph to display wrinkle data as a rotatable bar graph 11 Close to close the DropData awindow 12 Open the Show Photo window by selecting a photo in the Select Photo menu Wrinkle Analyzer TiVi90 3 3 Sida 50 TiVi90 Wrinkle Analyzer Hame LenaWR 0006 13 Copy to copy the photo to the windows clipboard from where it can be inserted into e g an Excel spread sheet 14 Tracks to display the tracks in the actual photo 15 Region to display the region of interest 16 Photo to display the entire photo 17 Close to close the Show Photo window Wrinkle Analyzer TiVi90 3 3 Sida 51 TIiVi90
17. Wrinkle Analyzer 6 5 Wrinkle Map Wrinkle Map Scan Through Scan Through EE ee NENNEN eee 1 Photo panel displays the ROI of the actual photo 2 Wrinkle panel displays the corresponding Wrinkle Map with the wrinkle segments highlighted and the background skin displayed in grayscale colour 3 File Name displays the actual filename 4 Remove Small Objects slider and edit box to remove small objects from the wrinkle map 5 Wrinkle Threshold slider and edit box to change the threshold level for wrinkle detection 6 Dragging the mouse pointer after pointing on a wrinkle segment and pressing the left mouse button to a position outside the Wrinkle Map to remove the actual wrinkle segment from the wrinkles Map 7 Undo Erase to restore the original set of wrinkle segments 8 Enumerate No Numbers to display or hide wrinkle segment numbers Wrinkle Analyzer TiVi90 3 3 Sida 52 TIVi90 Wrinkle Analyzer 9 Export Data to export wrinkle segment information to an Excel spread sheet 10 Load Settings to load saved wrinkle parameters Remove Small Objects and Wrinkle Threshold and apply these to the actual Wrinkle Map 11 Save Settings to save the current Remove Small Objects and the Wrinkle Threshold parameters 12 Scan Through to display the wrinkle segments one by one together with information about wrinkle segment number area and wrinkle
18. Wrinkle Appearance frame above the profile display Click the Wrinkle Structure button to display the structure of the wrinkle along the tracking area The Winkle Structure can be rotated by placing the mouse pointer within the wrinkle structure diagram and pressing the left mouse button Wrinkle Analyzer TiVi90 3 3 Sida 12 TiVi90 Wrinkle Analyzer 13 Click the Step Through button three times to display the wrinkle structures of the three tracks 14 Click the Wrinkle Profile button to again display the profile 15 Click the Save Session button Navigate to the TiVi90demonstration folder Print Asess in the File Name edit box and click the Save button to save the session under the name Asess TiVi9Osession 16 Click the Clear All button to reset the system 17 Click the Load Session button to open the Pick a File window Double click the Asess_TiVi90session file to re load this session 18 Click the Display Session button to open the Asess session in a separate window Move this window to the side it will be used as a reference window for placing the mouse pointer at the same wrinkles in other photos 19 Click Load Photo to load a new photo Double click the forehead 002 file to load this photo Wrinkle Analyzer TiVi90 3 3 Sida 13 TiVi90 Wrinkle Analyzer LEzpus Th Pra Frocsemcr Parmala Sathrequ Filmus Abzu Deno Anmissi Wrinkk Appearance 20 Draw a ROI around the area of
19. a 5 TIVi90 Wrinkle Analyzer 4 Endpoints setting the endpoints of the Wrinkle Profile are defined as starting from the upper and lower boundaries respectively The Wrinkle Volume is calculated as the area between the line that connects the endpoints of the wrinkle and the wrinkle profile The Wrinkle Asymmetry is calculated as the relation between the area to the left and the right of the horizontal line according to the formula 200 Left Area Right Area Left Area Right Area In the GETTING STARTED section these and other features are f rther explained by way of an example 3 GETTING STARTED The basic features of the Wrinkle Analyzer TiVi90 are probably best explained by way of an example In the following example it is assumed that the photos forehead 0001 jpg forehead 0006 jpg have been captured by a Canon EOS550D 650D TiVi camera with polarizing filters and stored in the 71Vi90demonstration folder These photos demonstrate wrinkles appearing in the forehead of a male subject with increasingly frowning forehead The task 1s to analyze the wrinkle appearance parameters depth volume and asymmetry and to display the wrinkle profiles and structures respectively 1 Open the Wrinkle Analyzer TiVi90 main window Wrinkle Analyzer TiVi90 3 3 Sida 6 TiVi90 Wrinkle Analyzer Egoi Purarerimrihatng Drepiwka Mimu About Demo Amistart Virinkla ppaaranca Phor Parameters Wrinkle Fonger Track P
20. aini Bie nuits Peri Sine Tract Len gin Track Vct Track Lini op inie Hip cari pi Trace Deptt Mode 2 Click the Parameter Settings pull down menu to open the Parameter Settings window Set the parameters according to the figure below by dragging the sliders or inserting the numbers directly into the edit boxes and check the Top check box in the Depth Calculation Mode panel The window should now look like Exon Parnmberscetings Manual Aut Deme Ardian Wrinkle Analyzer TiVi90 3 3 Sida 7 TIVi90 Wrinkle Analyzer 3 Click the OK button to store the parameters and close the Parameter Settings window 4 Click the Load Photo button and navigate to the 77Vi90demonstration folder Double click the file forehead 0001 file The TiVi90 main window should now look like Epot Parmele Settings DeopDsta Manual About Demo Assistant Its IL pipe Phot With i871 Haghe 11M Wrinkle Appearance Dm a Regign of irere Diy pS 13823511 Photo Psscamedprs Wrinkle Parameters Torehesd P001 Pha Track Deint Sm Guida Por Sca Tract Length Track Vite Trace Linit Er iadi Hz oud of Track Depth Vade 5 Draw a ROI as outlined in the figure below by use of the mouse position the pointer at the upper left corner of the ROI to be drawn press the left mouse button and drag the pointer to the lower right corner release the left mo
21. e segments are now renumbered Click the Export Data button and save the information about the wrinkles segments in an Excel file 12 Open the Excel file to display the wrinkle information File Name CrawFoot 0001 Remove Small Objects 6967 Wrinkle Threshold 139 0192 Number Area Wrinkle Score 1 oF TT 344 2 18403 2 0 3 457 A 14799 434 In order to make meaningful comparison of two photos with Craw foot wrinkles the parameters Removes Small Objects and the Wrinkle Threshold must have the same settings in both photos Wrinkle Analyzer TiVi90 3 3 Sida 42 TIVi90 Wrinkle Analyzer 6 DETAILED DESCRIPTION 6 1 Wrinkle Analyzer TiVi90 main window When the Wrinkle Analyzer TiVi90 is started in toolbox mode from inside the T Vi700 Analyzer the first photo in the sequence loaded in the 77Vi700 software is automatically transferred to the Wrinkle Analyzer TiVi90 In the Wrinkle Analyzer TiVi90 window the photo forehead 0001 jpg has been uploaded by clicking the Load Photo button and then double clicking the file icon forehead 0001 jpg in the TiVi90demonstration folder zr TWP ra Procemecr Parameter Settings Manual Absut Demo mustard Photo Wa d fi Hege Jia Wrinkle fuppeamsncn Duis raion TA ZR OI Phom Paraevirs Virinkle Parameters kreh Tr k Apaj Ene Gaus Pini Sates Track Leagh Track wisi Tak ara on Ecex Hz ceni ert Track Depi Vides The name of the photo is di
22. ee of this Application accepts the terms of the MCR Library License WheelsBridge is a registered trade mark Wrinkle Analyzer TiVi90 3 3 Sida 2 TIVi90 Wrinkle Analyzer 1 INTRODUCTION Wrinkles frequently appear on the parts of the body where there has been extensive sun exposure Wrinkles can appear as fine structures or deep furrows Smoking skin type heredity occupational and recreational habits are factors that all promote wrinkling of the skin Many products have been developed for the reduction of wrinkles or rather their appearance but few cost effective techniques are to date available for quantitative analysis of wrinkle appearance The Wrinkle Analyzer TiVi90 is a software package that analysis several features of wrinkles based on photos captured by polarization spectroscopy camera technology The intended use of the Wrinkle Analyzer TiVi90 1s to analyse the appearance of wrinkles in experimental in laboratory applications It is not yet approved for the diagnosis and treatment of disease 2 OPERATING PRINCIPLE The Wrinkle Analyzer TiVi90 operates 1n the following way 1 The TiVi camera is set to cross or co polarization mode by adjusting Illuminator filters 2 A photo of the actual skin area is captured 3 The photo is uploaded to the Wrinkle Analyzer TiVi90 main window 4 A region of interest ROI is drawn and displayed 5 Automatic tracking of a wrinkle starts by pointing at the wrinkle using t
23. gth determines the length of the track in horizontal pixels During a tracking procedure the tracking starts the mouse pointer after clicking the left mouse button and first tracks to the right half track length and then tracks to the left half track length The centre point of the track is marked by a green square 2 Track Width determines the number of vertical points around the latest track point used to calculate the next track point This is done by calculating the minimum value along the Track Width and uses this minimum value as the next track point 3 Trackpoint Size sets the size of the track point in pixels 4 Guidepoint Size sets the size of the Track Width points in pixels 5 Track Limit the maximum number of pixels the track is allowed to jump in the vertical direction in between two successive track points In operation only if the Narrow Track checkbox is un checked 6 Stop Index this parameter determines how well defined the minimum value along Track Width has to be to be classified as a true track point and not a random minimum value only In operation only if the Auto Stop checkbox is checked 7 Technically the Stop Index is compared to the relative difference of the minimum value and the average value along the Track Width A low value of Stop Index value is less tolerant to identifying a track point than a large value is 8 Number Out of Track sets the number of successive fail
24. he intensity projection of the photo between the two green boundaries constitutes a local profile of the wrinkle The tracking procedure is based on successively finding the minimum value of the intensity profile over the entire length of the track Based on these separate intensity profiles a wrinkle structure is composed displaying the intensity changes throughout the entire length of the track 150 i 160 140 EMI 120 s a 30 100 Track Length Track Width Wrinkle Analyzer TiVi90 3 3 Sida 4 TIVi90 Wrinkle Analyzer By calculating the average profile along the track a Wrinkle Profile is constructed 0 5 10 15 20 25 30 35 From this Wrinkle Profile several key features characterizing the wrinkle can be calculated The Wrinkle Depth is calculated as the length of the vertical line connecting the minimum value to the cross point of the line that connects the endpoints of the wrinkle The endpoints of the wrinkle can be defined in four different ways 1 Fix setting the endpoints of the Wrinkle Profile are defined as starting from the 10 pixel at each side of the minimum value 2 Top setting the endpoints of the Wrinkle Profile are defined as starting from the maximum value at each side of the minimum value Default 3 First Max setting the endpoints of the Wrinkle Profile are defined as starting from the first maximum value at each side of the minimum value Wrinkle Analyzer TiVi90 3 3 Sid
25. he mouse 6 The wrinkle profile is automatically calculated and features of the wrinkle appearance depth volume and asymmetry are displayed 7 The cross section of a wrinkle structure can be visualized in 3D 8 Up to 10 wrinkles in the same photo referred to as a session can be analyzed simultaneously 9 A complete session can be saved and uploaded again at a later occasion for further analysis 10 An infinite number of sessions can be displayed in separate windows simultaneously Wrinkle Analyzer TiVi90 3 3 Sida 3 TiVi90 Wrinkle Analyzer 11 The centre coordinates of a set of wrinkles can be saved and re loaded to be applied in other photos 12 The results of up to six sessions can be exported to an integrated Trend Analyzer for analysis of changes in wrinkle appearance over time 13 The overall result from the Trend Analyzer can be stored in a MS Excel spread sheet for further analysis 14 All basic recorded and calculated data can be stored in a MS Excel spread sheet 3 WRINKLE PARAMETERS The TiVi90 Wrinkle Analyzer automatically tracks a wrinkle white trace pointed at by the mouse within set boundaries green traces based on the local intensity within these boundaries of a photo captured by way of a polarization spectroscopy camera TP When atracking procedure is complete the track 1s assigned a number and a green indicator is placed at the centre of the track Each vertical line in t
26. ions Possibility to load and save wrinkle coordinates Operates in cross and co polarization mode All data can be exported to ASCII format spread sheets We are convinced that the Wrinkle Analyzer TiVi90 will be a productive tool in the assessment and follow up of wrinkle appearance following treatment with skin care products Thank you for choosing the WheelsBridge Wrinkle Analyzer TiVi90 WheelsBridge AB Wrinkle Analyzer TiVi90 3 3 Sida 1 TIVi90 Wrinkle Analyzer Information in this document 1s subject to change without notice and does not represent a commitment on the part of WheelsBridge AB No part of this manual may be reproduced or transmitted in any form or by any means electronically or mechanically including photocopying and recording for any purpose without the written permission of WheelsBridge AB Any use of application of WheelsBridge products must be performed by properly trained and qualified personnel WheelsBridge AB takes no responsibility for action taken on the basis of information derived from the use of the product described in this manual or from consequential damages in connection with furnishing performance or use of this manual Licensee s limited rights are governed by the license agreement between WheelsBridge AB and MathWorks Licensee may not modify or remove any license agreement file MarthWorks or third party that is included with the MCR Libraries MCR Library License Licens
27. se pointer at the centre of the actual wrinkles Wrinkle Analyzer TiVi90 3 3 Sida 20 TiVi90 Wrinkle Analyzer beet TW Ae eee Peer ete Wara Abs Dl Sid Wrinkle Appearance Warme TLE Volmw B Aaa T 8 Click the Save Session button and save the session under name HandA 9 Set Track Length to 20 and Track Width to 10 in the Parameter Settings window 10 Click Undo Show ROI and draw a smaller ROI in the photo cere A Prene Pee brings Pimak et Dema Aorta 11 Click the Show ROI button Wrinkle Analyzer TiVi90 3 3 Sida 21 TiVi90 Wrinkle Analyzer Exe TM Prelom Bete eto Mel Aban er Sete EZ 12 Click the Rotate Photo once to rotate the photo 90 degrees and get the finest wrinkles in parallel with the scanning direction 13 Produce tracks of the finest wrinkles by placing the mouse pointer at the centre of the finest wrinkles after having set the parameters Winks Appearance Vie Cage L1 i 1 Aga Md Wrinkle Analyzer TiVi90 3 3 Sida 22 TiVi90 Wrinkle Analyzer 14 Save the session under the name HandB 15 Click the Display Trend button to open the Trend window and load the two sessions See 24 uo DOR Iq Seana Hama amp Receding Data ceci apay ILrad Sessionis Export a Dist n C Siym Virinkdg Chae 16 The nghtmost group of bars represents the finest wrinkles while the leftmost group of bars represents the somewha
28. sets the TiVi90 system and prepares it for capturing or uploading a new photo 14 Save Coord to save the coordinates of the centre points of tracks and the associated ROI 15 Load Coord to load the coordinates of the centre points of tracks and the associated ROI 16 Adjust Coord to automatically make the final adjustment of green dots positions indica ng track centre points provided they are positioned in the vicinity of the actual wrinkles 17 Start Tracking to automatically start tracking if the associated green dots are in position Wrinkle Analyzer TiVi90 3 3 Sida 44 TiVi90 Wrinkle Analyzer Export TP re Procesror Para meler Seth gs Mangal Stout emo isyan Phot Lo Urin ke f rme ramce Tracking Complies Track Langit 101 WE Dph 24 Wolo 4 5 um E p m Jd LE Photo Parameters Track Pomel Soe Guide Poin Tira Ratte Pigg Track Largik Urie Shey All Track Ad Trak bL ang BHop mje Wa matol Track Depth cca The Wrinkle Appearance Index WAD Depth Volume and Asymmetry is displayed in the Wrinkle Appearance frame together with the number of the wrinkle profile structure displayed The large vertical bars represent the standard deviation while the shorter and thicker bars represent to standard error of the mean 18 Narrow Track to be checked if the track is to be continuous without any interruptions recommended With Narrow Track checked
29. splayed in the Load Photo edit box while the Height and Width of the photo in pixels are displayed in the upper right corner of the Photo frame together with the Date at which the photo was captured The next user action Draw a Region of Interest 1s displayed above the photo in the Photo frame 1 Edit box shows the name of the photo displayed 2 Load Photo to upload a photo from the hard drive 3 Wrinkle Map to open the Wrinkle Map window Wrinkle Analyzer TiVi90 3 3 Sida 43 TiVi90 Wrinkle Analyzer 4 Rotate Photo rotates the displayed photo 90 degrees counter clockwise To be used if the wrinkle to be analyzed runs vertically 5 Show ROI to display a region of interest if a ROI is drawn When this button is clicked the action can be reversed by clicking it again now labeled Undo Show ROD In the ROI photo the green frame indicates the borders for placing the mouse to start a tracking procedure 6 Abort clicking this button aborts the current track action 7 Undo Track erases the latest wrinkle track 8 Refresh erases all tracks and ROIs 9 Load Session to load a saved session 10 Save Session to save a session 11 Display Session to display the results in a new window the Display Session window Several Display Session windows can be kept open simultaneously 12 Display Trend to open the Trend window in which the overall results are displayed 13 Clear All re
30. t larger wrinkles in the skin at the base of the finger Click the Volume radio button to display the Volume of the two sets of wrinkles Volume armus Season bikita tir EO m St Su eg J I4 Sanam Kame A Receding Data Valume Whe Selact Depi Load Sessions spon t Depth a Malus 1 Ad yrmmmilry Anrijs Ch The final example demonstrates the possibility to save and reload the coordinates for a wrinkle session which is useful when two sessions recorded at different points in time are to be compared Wrinkle Analyzer TiVi90 3 3 Sida 23 TiVi90 Wrinkle Analyzer 1 Open the TiVi90 Wrinkle Analyzer main menu 2 Click the Load Photo button and select the forehead 0004 file in the TiVi90demonstration folder The main window should now look like Pho Wek T Haie 1328 Dam ap oe 3 Draw a region of interest click Show ROI and produce three tracks The main window should now look like Ret TAi pre Pisce ParamredsrisTireg Baa Aba Deme Senin Phain Tracks Compe Tracr Large 13i Wrinkle Analyzer TiVi90 3 3 Sida 24 TIVi90 Wrinkle Analyzer 4 Click the Save Coord button to save the centre coordinates of the three tracks and the associated ROI Navigate to a folder in which these parameters are to be saved insert a name MyCoord in the File Name text box and click the Save button
31. the track point is shifted no more than one pixel as the tracking proceeds from one vertical line to the next This facilitates the generation of a smooth track 19 Auto Stop to be checked if the track is to be automatically stopped when the wrinkle becomes too fuzzy 20 Wrinkle Structure click this button to display a 3D profile of the wrinkle in the Wrinkle Appearance frame Placing the mouse pointer on the structure changes the pointer to circular The view angle can be altered by moving the mouse while keeping the left mouse button pressed 2 p lt Wrinkle Profile click this button to display the average profile of a wrinkle in the Wrinkle Appearance frame 22 Step Through click this button successively to display either to Wrinkle Structure or the Wrinkle Profile of the actual tracks up to ten tracks allowed 23 Close to close the Wrinkle Analyzer TiVi90 window Wrinkle Analyzer TiVi90 3 3 Sida 45 TIVi90 Wrinkle Analyzer 24 Export to save the data associated with the tracked wrinkles in a MS Excel file ASCII format 25 Parameter Settings opens a new window in which the wrinkle tracker parameters can be set The actual numerical values of these parameters are displayed in the separate Wrinkle Parameters frame 26 Drop Data to open the Drop Data window into which wrinkle parameters can be automatically exported 27 Manual online version of the Wrinkle Analyzer TiVi90 manual 2
32. ures to identify a track point before the system determines that the wrinkle depth is too shallow to be dealt with If the Number Out of Track is exceeded and the system tracks to the right the actual tracking procedure stops and the system starts tracking to the left It the system tracks to the left and the Number Out of Track is exceeded the tracking procedure is completed and the profile structure and WAI values are calculated based on the reduced Track Length 6 3 TiVi 90 Wrinkle Analyzer Display window The Wrinkle Analyzer Display Session window is generated by clicking the Display Session button in the main window The Display window displays the result of a specific Wrinkle Analyzer TiVi90 3 3 Sida 47 TiVi90 Wrinkle Analyzer session An infinite number of Display Session windows can be kept open and used as references mee Winkie Appamasca VW E spon a4 Luz Meee IES kd y AB Aaa TE ely Zucht 1 Wrinkle Structure to display the wrinkle structure in the Wrinkle Appearance frame 2 Wrinkle Profile to display the wrinkle profile in the Wrinkle Appearance frame 3 Step Through click to manually step through the wrinkle structures or profiles 4 Close to close the Display Session window 6 4 TiVi 90 Wrinkle Analyzer Trend window The Wrinkle Analyzer Trend window is generated by clicking the Display Trend button in the main window The Trend window displays the overall results in terms
33. use button The TiVi90 main window should now look like Wrinkle Analyzer TiVi90 3 3 Sida 8 TIVi90 Wrinkle Analyzer Expat Tri Pe Processor Forarrnbus Settings pr Weih Time 6 Click the Show ROI button to display the ROI ipn o TMPPme Propescr Parameter bimg Marau Pici wren Lich prd mri bz ic gari kaing Dats Em might S 352g 2309 ai Sip Bee Hz caf af Tr ck Ges Uih Abc Dera dgucare 738 Heget mia Baka DS 18 55 11 Traci Pee Sire Gete Pont Son Truck Lan gir Track Pa Trick Leva Wa uro Track Cosh Elec Wanie Psramemrs Wrinkle Analyzer TiVi90 3 3 Sida 9 TiVi90 Wrinkle Analyzer 7 Control that the Narrow Track check box in the main window is checked and that the Auto Stop check box is unchecked 8 Position the mouse pointer on or in the immediate vicinity of the wrinkle in the upper right section of the photo and click the left mouse button to start the tracking procedure Isos OTM Pre Fropmegr Peramea Sirge Piece Abri Demo Amis Piisi fett Tob isi ii Winkle finpearance ech GF a ee i aii Packs Due Ad IMS isa Wink Paramater 9 The system will first track the wrinkle to the right of the mouse pointer and then to the left of the mouse pointer When the track is complete it is assigned a number and the average profile is displayed in the Wrinkle Appearance window The Wrinkle Appearance Index WAI indicates a depth of 19 2
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