Operator`s Manual
Contents
1. However because acetic acid is a weak acid basic urine specimens may require a greater number of drops of 2 acetic acid in order to lyse the red blood cells To lyse red blood cells using 2 acetic acid 1 Process the CenSlide tube as you normally would see Sections 2 5 thru 2 7 2 After the microscopic analysis has been performed uncap the CenSlide tube and decant the supernatant 3 Add 6 drops of 2 acetic acid to the CenSlide tube CenSlide 2000 Urinalysis System Page 3 5 Special Topics OPERATOR S MANUAL 4 Mix the acetic acid into the CenSlide tube s viewing area by bending it five times hand flicking it five times and then bending it five more times 5 Place the CenSlide tube in the StakRak horizontally and allow the urine sediment to settle for two minutes prior to microscopic examination Note Bending and hand flicking the viewing area causes an increase in the number of cellular elements in the viewing area Due to this increase quantitative analysis will not be valid as cellular element levels will be falsely elevated 3 3 Microscope Objectives 3 3 1 Background Examination of urine is normally done with a 10x objective for an overview evaluation followed by closer examination with a 40x objective Over the years it has become standard to use a 1 0 mm thick microscope slide with a glass coverslip of 0 17 mm thickness for the examination of urine under the microscope The optics in th2. Assure that the CenSlide tubes are properly placed in the CenSlide Holder Using the 10x Objective focus the microscope on the top or bottom of the CenSlide tube surface and then slowly adjust up or down until you find the observation plane Then switch to your 40x objective If it comes in contact with the CenSlide tube you will need to obtain a long working distance objective through your local distributor or directly through StatSpin If you have any questions or require customer assistance please call 1 800 782 8774 from 8 00 am to 6 00 pm EST CenSlide 2000 Urinalysis System Page 5 3 After Sale Support OPERATOR S MANUAL 6 0 StatSpin CenSlide Urinalysis System Warranty StatSpin warrants the CenSlide 2000 Centrifuge and CenSlide tubes to be free from defects in material and workmanship for one year During the first year StatSpin will repair or replace your CenSlide 2000 Centrifuge at no charge if it fails to perform as specified If the instrument has been modified without StatSpin s consent or if the failure is the result of misuse or abuse StatSpin has no obligation to repair or replace the failed centrifuge 6 1 StatSpin Maintenance Agreement After the first year a StatSpin Maintenance Agreement can be purchased to extend warranty coverage an additional year Under this agreement StatSpin will replace your centrifuge with a refurbished centrifuge at no charge if it fails to perform as specified If the Stat
3. At the end of the cycle an audible beep will sound the amber CYCLE light will go out the green READY light will begin blinking and when the rotor stops the lid will automatically open CenSlide 2000 Urinalysis System Page 2 4 Operation OPERATOR S MANUAL 2 7 Sample Analysis 1 Open the lid and remove the CenSlide tubes 2 Place the CenSlide tubes into the StakRak horizontally Figure 2 5 Allow the urine sediment in the tubes to settle for at least one minute prior to viewing 3 Place a CenSlide Holder onto the microscope stage 4 Place a CenSlide tube into the CenSlide Holder and position its viewing area under the micro scope objective Figure 2 6 Figure 2 6 5 Focus on the viewing area using the microscope s low power 10x objective and proceed with the microscopic examination Note Occasionally uneven distribution of the sediment may occur due to aggregates or clumps of debris mucous cells etc forming around the edges of the CenSlide tube s viewing area When this occurs count only the fields where elements are evenly dispersed Avoid counting areas inside the clumps CenSlide 2000 Urinalysis System Page 2 5 Operation OPERATOR S MANUAL 2 8 Quality Control There are a number of commercially available urine controls that contain white blood cells red blood cells and crystals that may be used for daily verification of the CenSlide Urinalysis
4. System The quality control procedures established for your laboratory should be followed CenSlide 2000 Urinalysis System Page 2 6 Special Topics OPERATOR S MANUAL This chapter covers special procedures technical specifications and references 3 0 Staining The recommended stain for use with the CenSlide 2000 is a modified Sternheimer Malbin stain This stain is commercially available call StatSpin for source 800 782 8774 This stain when used properly provides increased contrast and specific staining of urinary sediment This makes recognition of some formed particles especially casts easier 3 0 1 Preparing Censlide Tubes for Centrifugation 1 Label a CenSlide tube with the appropriate patient information 2 A first morning specimen with an acid pH is recommended because it is concentrated and more likely to contain casts than a dilute alkaline afternoon specimen Dispense a sample for analysis as follows a Shake the urine collection container sufficiently to resuspend the particles uniformly in the urine b Remove the lid from the urine collection container and pour the urine into the open end of a CenSlide tube up to the line directly above the arrow 5 mL 3 If requested insert a chemistry urinalysis test strip into the CenSlide tube prior to adding stain and tilt it slightly to assure that urine completely covers the uppermost pad 4 Shake the stain bottle before dispensing Add five to eight dr
5. balancing 7 For appropriate counter balancing simply place a CenSlide tube with equal volume of urine or water opposite each CenSlide tube placed in the rotor Figure 2 3 8 Seat the CenSlide tube firmly into the rotor slot pushing down on the cap until the CenSlide tube can go no further Close the lid The lid must be completely closed or the CenSlide 2000 Centrifuge will not operate When the lid is properly closed the green READY light on the control panel will stop flashing CenSlide 2000 Urinalysis System Page2 3 Operation OPERATOR S MANUAL 2 6 Centrifuge Operation 1 Press the green START button on the control panel of the CenSlide 2000 Centrifuge Figure 2 4 The amber CYCLE light will flash indicating that the centrifuge is currently in operation Figure 2 4 For safety purposes the lid will not open while the rotor is turning Microprocessor technology is used to monitor the speed and time to assure accuracy Near the end of the cycle a flicking process clicking sound will automatically occur to evenly disperse the sediment in the CenSlide tube Note If the OPEN ABORT button is pressed during operation the centrifuge will stop The amber CYCLE light will flash slowly and a beep will sound to indicate interruption of the cycle When the rotor stops the lid will automatically open It is recommended that the samples be discarded 2
6. time required to process and review a specimen The CenSlide 2000 Urinalysis System includes the CenSlide 2000 Centrifuge and CenSlide tubes Figure 1 1 Figure 1 1 The patented CenSlide tube is both a centrifuge tube and microscope slide eliminating the need for slides coverslips and specimen transfer Once a urine sample is dispensed into a CenSlide tube and the cap is put in place you never need to open the tube again greatly reducing the possibility of spills and contamination CenSlide 2000 Urinalysis System Page 1 1 Product Overview OPERATOR S MANUAL The CenSlide 2000 Centrifuge is microprocessor controlled to automatically spin CenSlide tubes for a preprogrammed time and speed At the end of the centrifuge cycle the urine sediment is redistributed evenly across the viewing area of the CenSlide tube completely ready for microscopic analysis The CenSlide tube inserts directly into a CenSlide Tube Holder which is located on the microscope platform The CenSlide Tube Holder positions each CenSlide tube in the same place on the microscope Once you focus on the first CenSlide tube each succeeding tube will automatically be in focus The urine sediment is easily visualized and counted through its patented transparent tip and correlates exceptionally well with conventional methods 1 1 CenSlide 2000 Cenirifuge The CenSlide 2000 Centrifuge Figure 1 2 is used exclusively with the CenSlide tubes to provide a rap
7. with highly refractile unstained globules within their hyaline matrix A dark purple nucleus with a large encircling border of light purple matrix Stain a light to medium purple Stain a light to medium purple Stain purple Stain purple 3 1 Confirmatory Protein Assay Note Do not perform a sulfosalicylic acid test until after the standard CenSlide microscopic analysis has been completed 1 Take the CenSlide tube used in performing the microscopic analysis and place it in a standard centrifuge 2 Centrifuge the CenSlide tube at 400 xg for five minutes CenSlide 2000 Urinalysis System Page 3 4 Special Topics OPERATOR S MANUAL 3 1 Confirmatory Protein Assay cont 3 After centrifugation remove the CenSlide tube from the centrifuge and decant the supernatant into a clean clear tube Be very careful when decanting the supernatant not to disturb the sediment at the bottom of the CenSlide tube 4 Add an equal volume of sulfosalicylic acid Exton s Reagent to the decanted supernatant and mix 5 Grade for cloudiness 3 2 Differentiating Red from White Blood Cells One of the most common methods of differentiating red blood cells from white blood cells is by the addition of a few drops of 2 acetic acid The acetic acid will lyse only the red blood cells leaving the white blood cells intact Red blood cells are effectively lysed in the CenSlide tube with the addition of 6 drops of 2 acetic acid
8. 0 C to 30 C CenSlide is a registered trademark and the CenSlide tube is a patented 5 260 032 product of International Remote Imaging Systems Inc CenSlide Power Supply Statspin Part No Description Mfg Model No 01 3553 001 AC Power Adapter APS AD 740U 1240 For use with all CenSlide 2000 centrifuges 01 3581 001 AC Transformer Leader A48240800 For use with 2C 9000 001 only CenSlide 2000 Urinalysis System Page 1 5 Operation OPERATOR S MANUAL This chapter covers the installation and general operation of the CenSlide 2000 Urinalysis System 2 0 Installing the Censlide 2000 Centrifuge After removing the CenSlide 2000 Centrifuge from its box place it on a level working surface close to an electrical outlet The CenSlide 2000 Centrifuge operates on 24 volts E supplied by an external power supply module Plug the power supply module into the electrical outlet and its power cord into the centrifuge s power receptacle located at the back of the centrifuge Figure 2 1 Mark a 300 mm clearance boundary around the centrifuge to allow adequate ventilation and safety Figure 2 1 Please use only the power supply provided with the equipment To turn unit completely off disconnect power plug in located at the rear of the instrument 2 1 Safety Features The CenSlide 2000 Centrifuge uses an electronically controlled locking mechanism to prevent the centrifuge from being 1 Operated except when the l
9. 2000 Centrifuge Warranty Card This will activate the warranty on your centrifuge and assure your receipt of pertinent follow up product information 1 Label a CenSlide tube with the appropriate patient information 2 A first morning specimen with an acid pH is recommended because it is concentrated and more likely to contain casts than a dilute alkaline afternoon specimen Dispense a sample for analysis as follows a Shake the urine collection container sufficiently to resuspend the particles uniformly in the urine b Remove the lid from the urine collection container and pour the urine into the open end of a CenSlide tube up to the line directly above the arrow 5 mL 3 If requested insert a chemistry urinalysis test strip into the CenSlide tube and tilt the tube slightly to ensure that urine completely covers the uppermost pad CenSlide 2000 Urinalysis System Page 2 2 Operation OPERATOR S MANUAL 4 f stain is required add it to the sample after the chemistry test strip has been removed and read Figure 2 2 5 Place the cap on the CenSlide tube and push down firmly until it clicks and or its rim is flush with the top of the CenSlide tube 6 Holding the CenSlide tube just Figure 2 2 below the cap align it so that the flat side of the CenSlide tube conforms to the flat side of the slot in the rotor head Figure 2 3 One to six CenSlide tubes can be placed into the rotor with appropriate counter
10. Controlled Lid and Bowl One piece molded Nylon plastic Centrifuge Cycle Ramp to 1350 rpm 410 15 seconds Hold 1350 rpm 15 seconds Ramp down to 400 rpm 15 seconds Flick tube 2 seconds Centrifugal Force 97 xg Power Consumption 20W Physical Dimensions Height 5 6 14 2cm Width 22k 11 4 29 0 cm Depth 12 9 32 8 cm Weight 9 lbs 4 1 Kg Environmental Conditions Indoor Use Altitude up to 2000m Temperature 5 C to 40 C Maximum relative humidity 80 for temperatures up to 31 C decresasing linearly to 50 relative humidity at 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient overvoltages according to installation category Il Pollution degree 2 RON ooo ea 6 Position fixed 45 angle Nylon plastic Switches Pressure sensitive membrane CenSlide 2000 Urinalysis System Page 1 4 Product Overview OPERATOR S MANUAL 1 4 CenSlide Tube Specifications Description Disposable non sterile shatterproof polypropylene plastic molded tube with clear viewing area incorporated Tubes are packaged with recessive leak resistant caps in shrink wrapped boxes Dimensions D shaped taper 110mm long x 21mm wide Viewing Area Dimensions 7 9mm w x 10 7mm I x 0 25mm h Total Volume 5 0 mL Storage Conditions Store at room temperature 2
11. Len5lide Urinalysis System OPERATOR S MANUAL Table of Contents OPERATOR S MANUAL Please read the entire Operator s Manual prior to operating the CenSlide Centrifuge This manual includes easy to follow instructions technical information about the CenSlide 2000 Urinalysis System maintenance instructions and a simple trouble shooting guide TABLE OF CONTENTS Product Overview 1 0 General zie RR ye rmx 1 1 1 1 CenSlide 2000 Centrifuge llus 1 2 1 2 CenSlide Tubes lille 1 3 1 3 CenSlide 2000 Centrifuge Specifications 1 4 1 4 CenSlide Tube Specifications 1 5 General Operation 2 0 Installing the CenSlide 2000 Centrifuge 2 1 2 1 Safety Features 0 000 cece eee eee 2 1 2 2 Precautions Lu eck Rr Ph ha eae Racal ra 2 2 2 9 Haza ie es be Reeth WOR Reus Pda ea anaes Cased 2 2 2 4 Preparing the Centrifuge for Use 2 2 2 5 Preparing CenSlide Tubes for Centrifugation 2 2 2 6 Centrifuge Operation 000 eee eee 2 4 2 7 Sample Analysis 00000 cee eee ee eee 2 5 2 8 Quality CONTON s arsana amani eee 2 6 Special Topics 3 0 SIAINING seac ee eNotes a WHat rax 3 1 3 0 1 Preparing CenSlide Tubes for Centrifugation 3 1 CenSlide 2000 Urinalysis System Page i 1 Table of Contents OPERATOR S MANUAL Special Topics Continued 3 0 2 Staining Characteristics liu 3 2 3 1 Confirmatory Protein As
12. Spin Maintenance Agreement is not purchased your centrifuge can be repaired or replaced at a fee to be determined at time of the repair or replacement 6 2 Technical Assistance For technical assistance call StatSpin at 1 800 782 8774 CenSlide 2000 Urinalysis System Page 6 1 Visit our home page on the internet at http www statspin com Stal Spr An IRIS Company 85 Morse St Norwood MA 02062 800 782 8774 781 551 0100 FAX 781 551 0036 REV C 3 98 55 3313 001
13. alk you through any procedures 4 0 Cleaning the Censlide 2000 Centrifuge For Urine Spills Clean up urine spills inside the CenSlide Centrifuge bowl with a cloth dampened with 5 bleach For Grease Makeup Clean the centrifuge with a damp Fingerprints or Stains cloth and mild detergent Note The bowl interior has not been designed to be fluid tight therefore disinfecting solutions should not be poured into the bowl 4 1 Cleaning the Censlide Tubes Wipe the viewing area surface lightly with clean gauze or lens cleaning paper Do not use disinfecting alcohol or water to clean the viewing area surface as these can cause water spots on the surface Note Be careful not to rub too strongly on the viewing area surface as this can scratch the viewing area CenSlide 2000 Urinalysis System Page 4 1 Maintenance OPERATOR S MANUAL 4 2 Censlide 2000 Cenirifuge Calibration The CenSlide 2000 Centrifuge uses microprocessor technology to monitor the speed rpm and timing to ensure the accuracy of each test No initial rom calibration is required The rom can be independently checked with a photoelectric tachometer available from many sources To check the rpm 1 Insert six 6 water filled CenSlide tubes into the rotor 2 Position the tachometer beam so it shines through the sight hole located at the top of the centrifuge cover 3 Turn the tachometer on 4 Press the start button wait 15 seconds and re
14. cord the rpm CenSlide 2000 Urinalysis System Page 4 2 Trouble Shooting Guide 5 0 Trouble Shooting OBSERVATIONS The CenSlide 2000 Centrifuge does not appear to have power OPERATOR S MANUAL ACTIONS Check that the power transformer is securely plugged into both the AC wall outlet and the back of the centrifuge Try a different AC outlet to verify that AC current is being supplied to the power supply module Make sure the lid is securely closed and locked in place Reset the CenSlide 2000 Centrifuge by disconnecting and reconnecting the power supply cord s plug to the centrifuge The CenSlide 2000 Centrifuge appears to vibrate when operating Balance appropriately by placing equally filled CenSlide tubes opposite each other It does not appear that the flicking mechanism is functioning CenSlide 2000 Urinalysis System Seat CenSlide tubes firmly and securely within the CenSlide 2000 Centrifuge rotor Check that you are using the correct power supply module Use only the power supply provided with the instrument Clean and dry the flicker button with alcohol and a tissue or swab Page 5 1 Trouble Shooting Guide 5 0 Trouble Shooting OBSERVATIONS OPERATOR S MANUAL ACTIONS Sediment appears to pack along the sides of the viewing area Seat CenSlide tubes firmly and securely within the CenSlide 2000 Centrifuge rotor If the specimen is extremely thick or turbid dilut
15. e objectives are ground to exacting tolerances which take into account the refractive index of glass which determines how much light rays are bent by a specific thickness With the advent of procedures such as tissue culture where the containers are usually of plastic and may be thicker than a glass coverslip it was found that the optics had to be modified so they were able to focus through the increased thickness and also deal with the refractive index problem of the various plastics being used These special objectives were more costly and did not find their way into the world of routine microscopes CenSlide 2000 Urinalysis System Page 3 6 Special Topics OPERATOR S MANUAL 3 3 2 Non American Optical Non AO Microscopes After 1979 the Deutsche Industrial Norm DIN Standard was accepted by most microscope manufacturers This standard ization of objective lengths solves the problem of the focusing distance when DIN Standard High Resolution Long Working Distance objectives are used Microscopes equipped with 40x DIN Standard objectives may hit the CenSlide tube s viewing area when it is rotated from the 10x objective to the 40x objective StatSpin invento ries a replacement 40x high resolution long working distance objective that produces excellent images at 40x without interference It can be used with all DIN Standard micro scopes Use StatSpin product number 2C 4950 001 Microscope Objective 40x Long to order thi
16. e the sample with saline CenSlide tubes leak The lid latch is stuck in the closed position CenSlide 2000 Urinalysis System Verify that the caps are firmly and securely in place on the CenSlide tubes The CenSlide tubes are not made for multiple use Use a non liquid filler to match the weight of 5 mL of fluid when using as a balance tube If the OPEN ABORT button does not release the lid after the cycle end alert disconnect the power cord at the back of the unit and then reconnect it to do a RESET Retry the OPEN ABORT button Place the bottom end of a Cen Slide tube into the slot in the lid latch and press against the latch Carefully clean and dry the latch assembly If the lid O ring is causing the lid to stick Clean it and the underside of the lid with mild soap and water Page 5 2 Trouble Shooting Guide OPERATOR S MANUAL 5 0 Trouble Shooting OBSERVATIONS ACTIONS The lid to the centrifuge is open Disconnect the power cord at the but the green light is not flashing back of the unit and then reconnect it This will cause a power RESET The yellow light will not go on Check that there is power to the when the start button is pressed centrifuge by observing the green light Check that the lid is securely latched Microscopic observation Make sure the CenSlide tubes have at least and focus difficulty a one minute settling time in the horizontal position after centrifugation
17. id and accurate urinalysis It automatically spins to the appropriate g force shuts off and flicks the CenSlide tubes Figure 1 2 viewing area to ensure even distribution of the sediment Rotor And C Lid CenSlide Slots Latch Open Abort Start Button Button CenSlide 2000 Urinalysis System Page 1 2 Product Overview OPERATOR S MANUAL 1 2 CenSlide Tubes Viewing Area The patented CenSlide tube Figure 1 3 is a combination tube and microscope slide The CenSlide tube reduces processing time and eliminates errors associated with decanting the super natant and resuspending the microscopic particles thereby increasing precision Cap and accuracy Figure 1 3 The CenSlide tube holds 5 mL of urine which is centrifuged and the resulting sediment is automatically resuspended in its built in viewing area for microscopic examination The CenSlide 2000 Urinalysis System has been clinically tested and shown to correlate favorably with tests performed by the standard microscope slide and KOVA methods CenSlide 2000 Urinalysis System Page 1 3 Product Overview OPERATOR S MANUAL 1 3 CenSlide 2000 Centrifuge Specifications Power Requirements Model Power Supply Mains M401 12 120 VAC 60 Hz 26 W Product No 2C 9000 001 M401 22 90 240 VAC 50 60 Hz 26 W Product No 2C 9000 22 Centrifuge Motor 24 VDC Brush Type Solid State Microprocessor
18. id is completely closed and latched or 2 Opened while the centrifuge is in operation IMPORTANT Do not attempt to defeat any safety A features of this equipment If the equipment is not used correctly protection provided by the equipment will be impaired To reduce the risk of injury Do not operate with an unbalanced load Inspect rotor for fatigue CAUTION Incorrect tubes may damage rotor NO USER SERVICABLE PARTS Refer all service to qualified service personnal CenSlide 2000 Urinalysis System Page 2 1 Operation OPERATOR S MANUAL 2 2 Precautions The CenSlide 2000 Centrifuge is designed to concentrate urine sediment in CenSlide tubes for diagnostic testing Therefore use of the CenSlide 2000 Centrifuge places a responsibility upon administrative personnel to ensure adequate training of operators as to the safe and effective use of the centrifuge 2 3 Hazards The CenSlide 2000 Centrifuge is not explosion proof and should not be used in a potentially explosive atmosphere 2 4 Preparing the Centrifuge for Use When the CenSlide 2000 Centrifuge is plugged in a tone will sound its lid will open about one eighth of an inch and the green READY light on the right side of the control panel will flash Open the lid fully by manually lifting it until it rests on its back support 2 5 Preparing Censlide Tubes for Centrifugation At this time remove complete and mail the CenSlide
19. ls Squamous Vaginal Urethral Cells Hyaline Casts RBC Casts Hemoglobin Casts WBC Casts blue with Renal Epithelial Casts Bacterial Casts CenSlide 2000 Urinalysis System OPERATOR S MANUAL Round cells with a blue nucleus and a large encircling border of pale blue cytoplasm These are ragged and irregularly shaped cells with orange to purple nuclei and extensive blue or purple cytoplasm Hyaline casts appear pink to light purple with uniform matrices They appear pale pink with unstained or pale lavender red blood cells enmeshed within their matrix They are deep purple and coarsely granular with mixtures of hemoglobin red blood cells and fibrin They appear pale pink with purple to orange white blood cells enmeshed within their hyaline matrix With glitter cell casts the cells are pale a granular cytoplasm surrounded by the pink hyaline matrix They appear pale pink with small cells with purple nuclei enmeshed within their hyaline matrix They appear pale pink with visible purple stained bacteria enmeshed within their hyaline matrix Page 3 3 Special Topics Granular casts Fatty Casts Trichomonas Vaginalis Budding Yeast Mucous Bacteria Mycelia Spores Fungi OPERATOR S MANUAL They appear pale pink with granules staining light purple fine granular or dark purple coarse granular enmeshed within their hyaline matrix They appear pale pink
20. ops of the stain to the CenSlide tube Figure 3 1 Figure 3 1 CenSlide 2000 Urinalysis System Page 3 1 Special Topics OPERATOR S MANUAL Warning This product will stain fabrics Use caution when dispensing 5 Place the cap on the CenSlide tube and push down firmly until it clicks and or its rim is flush with the top of the CenSlide tube Invert two to three times to evenly mix the specimen with the stain 6 Refer to Chapter 2 pages 2 3 through 2 5 for instruction on completing the preparation and reading of CenSlide tubes 3 0 2 Staining Characteristics Red Blood Cells In acid or neutral urine the red cells often remain intact and colorless or stain slightly purple In alkaline urine the red cells are usually lysed and alkaline hematin forms which stains dark purple White Blood Cells The nuclei of ordinary leukocytes usually stain a dark orange purple and the granularity of the cytoplasm is distinguishable There is variability between light and dark forms Glitter Cells These are large polymorphonuclear leukocytes frequently seen in pyelonephritis that appear pale light blue to almost colorless The cells are larger than dark staining cells Cytoplasmic granules with or without Brownian movement may be noted Renal Epithelial The nucleus stains dark purple with Cells small rim of orange purple cytoplasm CenSlide 2000 Urinalysis System Page 3 2 Special Topics Bladder Epithelial Cel
21. s objective 3 3 3 American Optical AO Microscopes American Optical microscopes equipped with a 40x infinity corrected objective do not have enough working distance to focus on urine microscopic elements without hitting the CenSlide tube s viewing area StatSpin inventories a replacement 40x high quality flat field long working distance objective that produces excellent images with all American Optical infinity corrected microscopes Use StatSpin product number 2C 4940 001 Microscope Objective 40x Short to order this objective If an American Optical microscope s 40x objective is Not Parfocal with the 10x objective it requires the operator to refocus excessively between the 10x and the 40x objective To eliminate this StatSpin supplies with each American Optical infinity corrected replacement objective a set of shims washers of different thickness that can be installed between the 40x objective and the turret to eliminate non parfocal situations Exact combinations of these shims provide the ability to fine tune the parfocal situation CenSlide 2000 Urinalysis System Page 3 7 Special Topics OPERATOR S MANUAL On an unmodified American Optical model 110 series microscope all shims will be required For a modified American Optical model 110 series microscope it may be necessary to remove some of the shims Remove the shims one at a time starting with the thinnest until i
22. say 4 3 4 3 2 Differentiating red blood cells from white blood cells 200200000 3 5 3 3 Microscope Objectives 0 00 eee ee 3 6 3 3 1 Background 22r eee Eds 3 6 3 3 2 Non American Optical Non AO Microscopes 3 7 3 3 3 American Optical AO Microscopes 3 7 3 3 4 Microscopes manufactured Prior to 1979 3 8 3 4 References 000 ces 3 8 Maintenance 4 0 Cleaning the CenSlide 2000 Centrifuge 4 1 4 1 Cleaning the CenSlide Tubes 4 1 4 2 CenSlide 2000 Centrifuge Calibration 4 2 TroubleShooting 5 0 TroubleShooting 000e eee eee eee 5 1 After Sale Support 6 0 StatSpin CenSlide 2000 Urinalysis System Warranty 0200000005 6 1 6 1 StatSpin Maintenance Agreement 6 1 6 2 Technical Assistance 2000000 6 1 CenSlide 2000 Urinalysis System Page i 2 Product Overview OPERATOR S MANUAL The CenSlide 2000 Urinalysis System is a closed system used in performing microscopic examinations of urine For n vitro diagnostic use 1 0 General The CenSlide 2000 Urinalysis System reduces exposure to biohazardous materials and helps laboratories control inherent variations in conventional microscopic analysis by standardizing urine sediment volume centrifuge conditions and technician technique Unique in its simplicity this advanced system is easy to use yet can dramatically reduce the number of steps and
23. t is possible to go back and forth between the 10x and 40x with minimal amount of refocusing Contact StatSpin Technical Service if assistance is required 3 3 4 Microscopes Manufactured Prior to 1979 Microscopes manufactured prior to 1979 have objectives that conformed to no standard Contact StatSpin Technical Service for assistance with these non standardized microscopes 3 4 References 1 Sternheimer R and Malbin B Clinical Recognition of Pyelonephritis with a New Strain for Urinary Sediment American Journal of Medicine 1 312 1951 2 Freeman J A and Beeler M F Laboratory Medicine Urinalysis and Medical Microscopy 2nd Edition Lea amp Febiger Philadelphia 1983 3 Tipper P L MS MT ASCP Yang G MS MT ASCP Nichols L MT ASC and Settineri R MS Comparison of CenSlide and Standard Methodology for Microscopic Urinalysis Diagnostic amp Clinical Testings Vol 28 No 2 pages 38 39 1990 4 NCCLS Document GP16 T Routine Urinalysis and Collection Transportation and Preservation of Urine Specimens Tentative Guideline December 1992 CenSlide 2000 Urinalysis System Page 3 8 Maintenance OPERATOR S MANUAL This chapter explains how to take care of your CenSlide tubes and CenSlide 2000 Centrifuge If you have any questions on how to use your CenSlide Urinalysis System simply call the StatSpin Hotline at 800 782 8774 A StatSpin representative will gladly answer your questions or t
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