LMC-3000 - User manual
Contents
1. CED Medical Biological Research amp Technologies LMC 3000 Laboratory centrifuge Operating Manual for versions Certificate V 5AD V 5AE Contents Oo ah ae eS S Salei PRECAUTIONS occas tasai a as a i a a a E a i a a 4 GENS Fal TOMMANOM acetone aidai as ii aa ai o a a a a a 6 STINGS S elgesi A I 7 Pr 10 dearer emer eee eee en AA ia a a AI 9 Speciel Saron aaa ass enthuses a a a eras heen a 11 Wire Ua oa 9 ey Lis sa ie eel E ge EO 13 Waranty and Claims 15 ai iii ia aaa i aaa L a etal ek ia 14 Declaration of Conformity Lisas sai i ia L aa eee a i 15 1 Safety Precautions The following symbols mean A Caution Make sure you have fully read and understood the present Manual before using the equipment Please pay special attention to sections marked by this symbol GENERAL SAFETY Use only as specified in the Operating Manual provided Do not use a dropped or damaged unit Store and transport the unit in a horizontal position see package label After transportation or storage and before connecting to electric circuit keep the unit under room temperature for 2 3 h According to EN 61010 2 20 people and hazardous materials must not be within a 300 mm area around the device during the centrifuge operation Use only original accessories rotors adaptors etc provided by the manufacturer and ordered specifically for this model Before using any cleaning or decontamination methods except those recommen2. 4 Fuse holder 7 Warranty and Claims 7 1 The Manufacturer guarantees the compliance of unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions 7 2 All rotors and accompanying metal buckets have maximum operating life of 7 years from the date of use The plastic buckets have an operating life of two years form the date of use 7 3 The warranted service life of unit from date of delivery to the Customer is 24 months excluding the consumables i e adapters see tables 2 and 3 Contact your local distributor to check availability of extended warranty 7 4 Warranty covers only the units transported in the original package 7 5 If any manufacturing defects are discovered by the Customer an unsatisfactory equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form 7 6 The following information will be required in the event that warranty or post warranty service comes necessary Complete the table below and retain for your records LMC 3000 Laboratory centrifuge Serial number O Date of sale O 8 Declaration of Conformity Declaration of Conformity Equipment name Type of equipment Directive Manufacturer Applied Standards LMC 3000 Laboratory Centrifuge EMC Directive 2014 30 EC Low Voltage Directive 2014 35
3. R 12 10 For 12 of 4 8 ml vacutainers xH 13x100 mm BS 010208 QK BN 16 100 R 12 10 For 12 of 8 9 ml vacutainers xH 16x100 mm BS 010208 RK AP 96 mitopaies tia 196108 948 Ti san irae aTa Table 3 Replacement parts rotor adapter sets asi porrer sein Sa ano nae SPER Tag esameak For 12 of 15 ml centrifuge tubes BN 17 120 R 12 15 xH 17x120 mm BS 010208 TK For 12 of 10 15 ml centrifuge tubes BN 16 90 R 12 10 xH 16x90 mm BS 010208 SK 12 Relative Centrifugal Force 6 1 6 2 6 3 6 4 Maintenance If the unit requires maintenance disconnect the unit from electric circuit and contact Biosan or your local Biosan representative All maintenance and repair operations must be performed only by qualified and specially trained personnel Standard ethanol 75 or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and disinfection of the unit It is recommended to perform disinfection after operation session by cleaning the parts inside the centrifuge chamber The rotor and other accessories are autoclavable 120 C 20 min Fuse replacement Disconnect from electric circuit Remove the power plug from the rear of the unit Pull out the fuse holder by applying leverage in recess A Remove the fuse from the holder Check and replace with the correct fuse if necessary for 230 V M1 A or for 120 V M 2 A type M time lag Medium Fig
4. the new rotor carefully by turning the fixation nut tightly 4 Operation Recommendations during operation A 4 1 4 2 4 3 44 4 5 4 6 Use even numbers of tubes arranged symmetrically one opposite another when loading to give the unit even balance during operation The opposite tubes must be filled up egually Centrifuge rotors must not be filled over the volume specified by the manufacturer Rotor must always be fixed securely Stop the operation immediately by pressing the RUN STOP key if any unusual noise occurs during acceleration which can be due to improper rotor fixation Check the power cord preliminary for any signs of damage Connect the power cord to a properly grounded power socket Set the power switch on the rear side to position ON The centrifuge will turn on and the lid opens automatically The following readouts appear on the display previously set time and speed in the upper line Set mode indication STOP lid closed and il lid locked or OPEN lid is open rotor stopped and current speed 0 rpm in the lower line Actual Check the rotor and buckets for any signs of wear or corrosion and replace if necessary Insert EVEN number of tubes microtest plates in rotor one opposite another The loading in the opposite tubes must be equal Using the A and V TIME keys fig 3 1 set the required time interval 0 90 min increment 1 min Using the A and V RPM keys fig 3 2 se
5. EC RoHS 2011 65 EC WEEE 2002 96 EC amp 2012 19 EU SIA BIOSAN Ratsupites 7 build 2 Riga LV 1067 Latvia EN 61326 1 Electrical equipment for measurement control and laboratory use EMC requirements General requirements EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61010 2 20 Particular requirements for laboratory centrifuges We declare that this product conforms to the requirements of the above Directive s Signature of 4 AHA Signature Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R amp D 28 01 2015 Date 72 01 2015 Date 15 Biosan SIA Ratsupites 7 build 2 Riga LV 1067 Latvia Phone 371 67426137 Fax 371 67428101 http www biosan lv Edition 5 01 July 2015
6. chieved stable indication RUN Note If the rotor imbalance occurs causing vibration the centrifuge stops automatically IMBALANCE indication will be shown on the display In such a case open the lid after the rotor has stopped and remedy the cause of imbalance Centrifugation is stopped automatically after the set time elapses while display shows blinking indication STOP A sound signal is emitted after full stop of the rotor press the RUN STOP key fig 3 3 to stop the signal Centrifugation can be stopped before the set time elapses if necessary by pressing the RUN STOP key The set time interval will be shown on the display Press the A Open key fig 3 4 and open the lid lifting it upwards with a hand it is possible to open the lid only when the rotor is stopped Display shows OPEN At the end of operation set the power switch in position O OFF on the rear Disconnect the power cord from electric circuit Note The electrical lid lock allows opening the lid only when the unit is connected to the mains and is turned on Do not force the lid to open when the unit is switched off 4 14 Lid emergency opening 4 14 1 Disconnect the power cord from electric circuit 4 14 2 Slide the unit to the front of the bench to access the emergency opening slot on the underside of the unit located in the front side Avoid tilting the unit as this may cause spilling of the materials from the containers inside the unit 4 14 3 Insert a s
7. ded by the manufacturer check with the manufacturer that the proposed method will not damage the equipment Do not make modifications to the design of the unit ELECTRICAL SAFETY Connect only to electric circuit with voltage corresponding to that on the serial number label Ensure that the switch and plug are easily accessible during use Do not plug the unit into an ungrounded power socket and do not use an ungrounded extension lead Disconnect the unit from electric circuit before moving Switch the unit off and disconnect the power cord plug from power socket to disconnect the unit from electric circuit It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment If liquid penetrates into the unit disconnect it from electric circuit and have it checked by a repair and maintenance technician Do not operate the unit in premises where condensation can form Operating conditions of the unit are defined in the Specifications section DURING OPERATION Do not centrifuge flammable or chemically active substances If such liquids are spilled on the rotor or rotor chamber the centrifuge must be cleaned with a most cloth and a mild soap solution Do not use rotors with visible signs of corrosion wear or mechanical damage Do not fill in the tubes after they have been inserted in the rotor Do not leave the operating unit unattended Do not operate the uni
8. eeessaaeeeeeeees 1 copy Optional accessories PO TOLO Bursa a a r S R on reguest ReOP rolor eiaa aE TR on request aa BO OO L o E E A E E on request AL OD EEE EFF on reguest gt ONO gt Pm pr ssp ps pP on reguest R 24GC rotor for gel cards ccccccccccccceeceeeeaeneseseeeeeeeeeeeeeeeees on request RR U rotor support stand B aaa aaa aaa aaa a aaa aaa on reguest BN 13 75 BN 13 100 and BN 16 100 for R 12 10 and BN 11 30 for R 6P adapter sets B on reguest 3 3 Set up place the unit on the even stable and clean surface remove protective film from the display plug the power cord into the socket on the rear and position the unit so that there is easy access to the power switch and mains according to EN 61010 2 20 people and hazardous materials must not be within a 300 mm area around the device during the centrifuge operation do not place any objects 100 mm behind the centrifuge to ensure unimpeded air circulation Rotor replacement Caution Check the rotor and adapters for any signs of wear or corrosion and replace if necessary Hold the rotor with one hand and using the supplied wrench for rotor replacement 13 mm turn the fixation nut fig 2 1 counterclockwise to release the rotor Caution Do not hold the rotor by rings or adapters mounting when mounting and fixing it Hold the rotor as shown on Fig 2 Correct Correct 1 Fig 2 Rotor fixation Replace the rotor and secure
9. l lid automatic imbalance switch off lid lock during the centrifuge operation provide safe operation at all speeds e Rotor imbalance automatic diagnostics emergency stop indication IMBALANCE e Low noise level not more 60 dBA e Soft run up and run down of the rotation e Possibility to switch off forced braking e Wide choice of accessory rotors Centrifugation process causes moderate heating of the operating chamber which does not exceed 15 C above room temperature during one and a half hour In case where quickly inactivating samples are to be centrifuged the preliminary cooling or use of LMC 4200R refrigerated centrifuge is recommended 3 1 3 2 Getting started Unpacking Remove packing materials carefully and retain for them future shipment or storage of the unit Examine the unit carefully for any damage incurred during transit The warranty does not cover in transit damage Warranty covers only the units transported in the original package Complete set Package contents Standard set LMC 3000 Laboratory centrifuge ccccccceeseeeeeeeeeeeeeeeeeeeeeeeeeaaeaees 1 pce Spare fuse inside the fuse holder ce eseeeeeceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeas 1 pce BOWE I COU oresar a ro ae saaa iais aa a asis aaa aaa sio 1 pce wrench for rotor replacement 13 MM cccccsseeeeceeeeeeceeeeeeseeeesaaeees 1 pce Operating manual Certificate cccccceeeeeccceeeeeeeeseeeee
10. mall screwdriver or similar tool with diameter up to 3 mm into the emergency opening slot in front of the dot on the label Open at a depth of 10 15 mm 4 14 4 Move the lever to the arrow direction to release the lid lock 5 Specifications The unit is designed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C in a non condensing atmosphere and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 5 1 Speed setting range aaa 100 3000 rpm increment 100 rpm 5 2 Digital time Setting ceeccesseeeeeeeeeeeeeeeeeees 1 90 min increment 1 min 5 3 Acceleration time up Max speed NOt more cccceeeeeeseeeeeeeeeeeeeeeeeeens 20s 5 4 Deceleration time TORCEG braking NOMOS sereine e a a ai a i k aa a a ai L 35s THES DEAKINGs NOLO ix kasatorius a lia a as i a i Oa 100s 5 5 Rotor imbalance automatic CIAQNOSTICS cccceeeeeeeeeeeeeeeeeens emergency stop indication IMBALANCE 5 62 sJ ROLAMOM drechoN ik is beac eecorcece eal see omsies eeork seumeereeeenie counterclockwise ihc WIS DIA A ae a sia deaasas eccetule ashes eaters LCD 5 8 Maximum noise level not More cece eseeseeseeseeeseesueeeeveeeeaeeaess 60 dBA 5 9 WOKO GIAIMCIOK ais cece asas ses eaten crated ne ai i A aa 335 mm STO DIMENSIONS aisiais os sodas aa saaa aaa ia 495x410x235 mm 5 11 Operating voltage power co
11. nsumption 230 V 50 Hz 110 W 0 5 A or 120 V 50 60 Hz 120 W 1 A SA2 Weni issue eee ia aa a ala a e ia aa A a 11 8 kg Biosan is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice Accurate within 10 Table 1 Rotors and rotor holder Tube plate Model Description and gel card Gatalogue number manufacturer For 6 conical centrifuge tubes of 50 ml with aluminium tube holders xH 40x103 mm BS 010208 DK max speed RCF 3000 rpm 1700g For 6 conical centrifuge tubes of 50 ml with plastic tube holders xH 40x103 mm max BS 010208 XK speed RCF 3000 rpm 1700g For 12 conical centrifuge tubes of 15 ml Bea BS010208EK R 12 15 xH 17x120 mm Bi BS 010208 EK io one Nunc max speed RCF 3000 rpm 1700g Sarstedt For 12 round bottom centrifuge tubes of 10 15 i PRCI 300 pa max speed RCF 3000 rpm 1700g For 2 standard 96 well microplates R max speed RCF 2000 rpm 560g BS 010208 AK LxWxHmax 128x85 6x45 mm For 24 of 8 column gel cards for blood group R 24GC serology testing max speed RCF Grifols Diamed BS 010208 VK 1500 rpm 375g LxW 53x74 mm RR U Rack for rotors BS 010208 UK Table 2 Optional accessories rotor adapter sets ae Catalogue For rotor Description umber R 12 10 BN 13 75 R 12 10 For 12 of 2 5 ml vacutainers OxH 13x75 mm BS 010208 PK BN 13 100
12. t in environments with aggressive or explosive chemical mixtures Please contact manufacturer for possible operation of the unit in specific atmospheres Do not operate the unit if it is faulty or has been installed incorrectly Do not use outside laboratory rooms Use only cleaning and decontamination methods recommended by the manufacturer BIOLOGICAL SAFETY According to EN 61010 2 20 a centrifuge without a gasket is not considered a biologically safe system and therefore cannot be used for centrifuging hazardous materials contaminated with toxic radioactive or pathogenic microorganisms It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment 2 General information LMC 3000 is a modern desktop laboratory centrifuge useful for sedimentation of cells bacteria yeast formed blood elements It provides operation with tubes gel cards and microtest plates LMC 3000 is designed for safe work metal protecting housing easy maintenance and wide application range in medical biochemical industrial and other type laboratories Opening for external Fuse speed control holder Power socket Power switch Control panel Fig 1 Overall view FEATURES e User friendly centrifugation parameters time and speed input and simultaneous display of the set and actual parameter values e Safe assay performance metal protective housing and meta
13. t the required speed 100 3000 rom increment 100 rom Note that the speed can also be adjusted during operation Note Some plastic tubes and microtest plates can be damaged at higher speeds Refer to the tube material specifications to make sure that it will not get damaged at the set speed Do not set speed higher than 2000 rom when working with microtest plates or 1500 rom when working with gel cards Changing the braking mode of the rotor By default centrifuge applies force to stop the rotor after program completion To toggle between forced braking and free braking press A Open key fig 3 4 and hold it for 8 s Using VY and A TIME keys chose between BRAKE OFF free braking n BRAKE ON forced braking Press and hold A Open key for 8 s to exit choosing mode 4 7 4 8 4 9 4 10 4 11 4 12 4 13 10 Time min sec Speed RPM RPM LMC 3000 RUN STOP Fig 3 Control panel Close the lid carefully and smoothly until the clicking sound is heard STOP indication and ia symbol appear in the lower line of the display fig 3 5 Note If any of these readings STOP or has not appeared on the display the program will not start centrifugation Try to open and close the lid again Press the RUN STOP key fig 3 3 to start centrifugation Blinking indication RUN and current speed is displayed in the lower line The timer in the upper line starts countdown after the set speed is a
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