tacoTM Instrument user manual
Contents
1. Laminar air flow optional Disposable gloves Protective goggles Lab coat Note To avoid power failure connecting the taco 32 to an UPS is recommended 2 4 Front View of taco 32 2 4 1 LED Signal Signals Run Stand by signal Green Pause End signal Yellow UV on signal Green Error signal Red taco 32 Nucleic Acid Automatic Extraction System un Stand by Pause End O 1 UV 2 e Self test passed Ready to run Lights on Extraction finished Lights on UV lamp on Lights on Any error occurred taco 32 Nucleic Acid Automatic Extraction System 2 4 2 Operation Button Pause Reset Function disabled under the No Button Description following status During the extraction 1 Door Press to open close the door UV lamp is on During magnetic bead transfer Short press to pause the During magnetic bead drying machine UV lamp is on 2 Pause Reset While paused or extraction Reset is functional only when finished long press to reset extraction is paused or finished the machine Press to start resume the 1 Door is open 3 Start l machine 2 UV lamp is on taco 32 Nucleic Acid Automatic Extraction System 2 5 Rear View of taco 32 F taco 32 Nucleic Acid Automatic Extraction System 2 6 Loading taco Plate amp Sleeve 2 6 1 Loading the 96 Well Extraction Plate Magnetic rod module Shipping anchor of the mag2. off the UV lamp press the UV light switch again Note Under the circumstances listed below the UV light switch is nonfunctional Reset is not done after extraction The door of taco 32 is open During the extraction process 4 5 How to Shut Down taco 32 4 5 1 Before switching off taco 32 please make sure the 96 Well Extraction Plate and Mixing Sleeves are removed from the device 4 5 2 Press the door button to close the door 4 5 3 Turn the main switch off 11 5 Cleaning and Maintenance 5 1 General Care Do not disassemble taco 32 for cleaning This will void the warranty Never allow liquids to sit on any surfaces of the device for extended periods of time Do not use solvents detergents or bleaches to clean the magnetic rods Use only clean and wet paper towel to wipe off stains on magnetic rods Note Wear gloves or other protective gears Always turn off and unplug taco 32 before cleaning 5 2 Dealing with Spills Wipe off any visible stains on the cleaning area see illustration below with disposable paper towel immediately In case of serious liquid spills please contact your local distributor or GeneReach for assistance Do not dismantle the instrument for cleaning Please make sure the total volume of each well does not exceed 900 ul before extraction Using UV light for sterilization after each extraction is recommended Cleaning area 12 Troubleshooting taco
3. 32 does not perform normally a No LED light signals b Self test too long c The magnetic rods module and lower racks are not in the correct positions d Unusual noises from the machine e Liquids spill and adhered to device Magnetic rod function failure a Stains on the magnetic rod b The magnetic rod comes off from the heat shrink sleeve Comments and Suggestions Check to see if the plug has been connected to the power system Check to see if the main power has been switched on Check voltage of electricity outlet see 2 2 taco 32 Specifications Contact your local distributors or GeneReach for assistance Contact your local distributors or GeneReach for assistance Contact your local distributors or GeneReach for assistance Check the Mixing Sleeves to see if they have been pushed into the lower rack Check the 96 Well Extraction Plate to see if it has been pushed into the plate holder Check that there is nothing preventing the track from moving Contact your local distributor or GeneReach The total volume of each well should not exceed 900 ul Use UV light for sterilization and then clean taco 32 with water or a laboratory detergent Repeat the extraction procedures with new samples and reagents Ensure the Mixing Sleeves are installed before each extraction Use clean and wet paper towel to wipe it out carefully Do not use any solvent or detergent to clean the magn
4. GeneReach immediately Any damaged components may lead to poor performance of the instrument 3 1 Safety Information Not following the instructions may cause personal injury or device failure Place taco 32 in a room with good ventilation Make sure the working area is clean dry flat and vibration proof leave enough room for cables lids and other accessories Do not touch any plugs and electric switches with wet hands Make sure to keep taco 32 away from water Do not unplug by pulling on power cord Hold the plug not the cord to unplug Keep power cord away from any hot surface Turn off and unplug the machine before moving it Package taco 32 carefully before moving and or delivery Vibrations or crashes may lead to damage Users should revalidate taco 32 if the device has been moved Waste disposal must be in compliance with local laws When handling infectious materials always wear a lab coat disposable gloves and protective goggles Do not look straight at the UV light while lights are on Do not repair the machine yourself including fixing the door and changing the UV lamp Contact your local distributor or GeneReach directly to deal with the problems 3 2 Emergency Stop 3 2 1 Should any emergency occur press the PAUSE button or turn off the MAIN SWITCH to stop taco 32 3 2 2 After all emergency is cleared please follow the instruction to resume
5. ees l1 A SOW TO ShutDown Taco M S oenen EE EE ETONE EE aR EE Eren E E 11 5 Cleaning and Maintenance esesssessececececssesecescesesecessesesesessesesesecsesesesesseseseseo 12 ARTEO E e E E ETE E E E EE 12 32 DCA Vy MN OI EG a cei cscule doce acegwiasue E E ER 12 Trouble hooo cocerscckcessccecccevscevscessecrscevacssacesscenecexsesvabeccsceetssicesanssssenctsvecssstaceecestsvtustscacisesedesssassesbers 13 Symbols xs Pe HAEE taco 32 Nucleic Acid Automatic Extraction System Date of manufacturing Manufacturer Catalog number Serial number Alternative current Consult instruction for use Caution consult accompanying documents This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE symbol WEEE Directive 2002 96 EC Please follow your institutional requirements for disposal of the accessories taco 32 Nucleic Acid Automatic Extraction System 1 Introduction The taco 32 Nucleic Acid Automatic Extraction System taco 32 is a magnetic bead based DNA extraction and purification device Nucleic acids bind to silica coated magnetic beads which can be separated easily from biochemical contaminants through automatic transfer and processing of magnetic beads Purified nucleic acids could be eluted from the beads after several washing steps 1 1 Intended Use taco 32 is developed to work with nucleic acid extraction kits developed by GeneReach Bio
6. etic rods Contact your local distributor or GeneReach 13 UV Lamp function failure UV light signals not on Door function failure The door of taco 32 cannot be opened closed normally Comments and Suggestions Check that the door is closed The UV lights are designed not to operate while the door is open Check to see if taco 32 is in extraction mode UV lights cannot operate during extraction Contact your local distributor or GeneReach for assistance Contact your local distributor or GeneReach for assistance 2014 GeneReach Biotechnology Corporation All rights reserved For research use only Not intended for any animal or human therapeutic or diagnostic use 14
7. netic rod Housing for the Mixing Sleeve _ Protection of magnetic rods and mixing the sample Mixing Sleeve with magnetic bead Deep well plate for extraction Use two Mixing 4 96 Well Extraction Plate Sleeves for each 96 Well Extraction Plate when processing extraction The 96 Well Extraction Plate should be fully inserted Notched corner of the 3 5 into the plate holder with the notched corner pointing 96 Well Extraction Plate to far right 6 Plate holder Housing for the 96 Well Extraction Plate Note The Mixing Sleeves and the 96 Well Extraction Plate should be placed in the same slot when processing extraction See illustrations above Inadequate installation of taco Plate amp Sleeve may damage the machine taco 32 Nucleic Acid Automatic Extraction System 2 6 2 Loading the Mixing Sleeve Slide the Mixing Sleeve into position Make Tenon of Mixing Sleeve sure the tenon on the Mixing Sleeve is inserted fully into the mortise on the lower rack 2 6 3 Releasing the Mixing Sleeve d Button Mortise Button of Mixing Sleeve Press the button to Mixing Sleeve to release Note Using non recommended consumables may affect the device performance taco 32 Nucleic Acid Automatic Extraction System 3 Important notes After receiving taco 32 please check the instrument and accessories for any damages Should any questions arise please contact your local distributor or
8. restart the taco 32 See 4 1 amp 4 2 taco 32 Nucleic Acid Automatic Extraction System 4 Operation Procedure of taco 32 4 1 How to Start the taco 32 4 1 1 Load the reagent to 96 Well Extraction Plate in advance See taco nucleic acid extraction kit manuals for instruction Note Please make sure the total volume of each well does not exceed 900 ul before extraction 4 1 2 Plug in taco 32 and switch on the main power The device will complete self check within 3 minutes and be ready when the Run Stand By signal lights are on 4 1 3 Press the door button to open the door of taco 32 load 96 Well Extraction Plate and Mixing Sleeves according to the instructions See chapter 2 6 Loading Plate amp Sleeve 4 1 4 Push the 96 Well Extraction Plate and Mixing Sleeves into the plate holder and lower rack respectively Ensure they are in the correct position 4 1 5 Press the door button of the taco 32 to close the door 4 1 6 Press START to begin the extraction Note Operate taco 32 only under room temperature 16 30 C Note Under the circumstances listed below the START button is nonfunctional The UV lamp is on While the door of taco 32 is open 4 2 How to Pause taco 32 4 2 1 During the extraction press the PAUSE button to stop taco 32 if necessary The yellow LED will flash while the device is paused 4 2 2 The magnetic rods module and Mixing Sleeves
9. taco 32 Research Use Only OOOO 0 0 0 0 ooo Nucleic Acid Automatic Extraction System User Manual Manufacturer GeneReach Biotechnology Corporation TEL 886 4 2463 9869 Email sales genereach com No 19 Keyuan 2 4 Road Central Taiwan Science Park Taichung City 407 Taiwan Website www tacomag com Cat atcd 0O1 2014 03 Content SV UNS a a S 1 Linodic ions cen cecceseveivssceusecsccusteiecedeusesecveneiwssceudesscueneivcuveuvesecuseusscsersesesvsade 2 enged U e E ETT E 2 Lero M Z Exron Pinciples E Eei 2 O DZ Ea a 3 TAPO T OPO MLS e o E E stuns 3 PAT E aa e S EE 18 A E T EE E E A E E 3 2 3 Equipments to Be Supplied by USer 2 0 eee ccccccccesssesseececeeeeeaeessseeeeeeeeesseeeeeeeeeeeeesaeeeesses 3 LATO VAC WOT ARCO cS 2 cerns siyasecistoase n N T A aeeadesseesdeensseusveosteatse EE 4 De DIG al ACW OL a M a a E E E EA NE EAEE 6 20 Loadine taco ABest nace cscierawece aso aucaanenveannassisededieen aSa AE EOE nenesa 7 sporan IN OUCS aa cevasssyesscssecsscauegsseigeccscenyacsr ss tavesuengsssesvavesescersssdveseteveseensssas lt varecaseeese 9 Ded SACL MINOR aE i siccse chore E E EE N E 9 EME O A E E to deein eect saaiens daauanessaceredesens 9 A Operation Procedure of taco OZ acc scceescacsss vers cececesnussicssececussusss AEE a Ear N 10 ALHO O SCO e eE E E 10 P WOH E a E A N E A ET 10 to How OR sc aco M A E a E E E 11 4 4 When and How to Turn on UV Lamp ccccecesssseecccecceeeeeseeececeeeeaaeeseececeeeesaaeaseeeee
10. technology Corporation GeneReach to extract nucleic acids from samples for research use purpose This product is intended for professional use by properly trained laboratory technicians familiar with molecular biology techniques 1 2 taco Extraction Principle taco 32 is based on the magnetic separation technology to work with the specially designed disposable taco Plate amp Sleeve which are provided with all reagent kits Samples and reagents including the magnetic particles are dispensed into wells of the extraction plate manually according to instructions of the kit before extraction starts The automated steps performed by taco 32 include Transfer of magnetic beads and mixing magnetic beads with samples Sample lysis Binding of nucleic acids to magnetic beads Washing steps to remove biochemical other than nucleic acids Elution of nucleic acids from magnetic beads taco 32 Nucleic Acid Automatic Extraction System 2 taco 32 Layout 2 1 Product Components Component Quantity l taco 32 Power Cord User Manual Warranty Card 2 2 taco 32 Specifications Specification Dimensions W x D x H 42 cm x 30 cm x 38 cm Net Weight 23 4 kg Power Requirement 100 120 200 240V AC 50 60 Hz 2A Operating Temperature Extraction time 2 3 Equipments to Be Supplied by User taco nucleic acid extraction kit taco Plate amp Sleeve Uninterrupted power system UPS optional
11. will move up to the top together 4 2 3 Close the door of taco 32 and press Start Resume to resume the extraction Note Under these circumstances listed below the Pause button is nonfunctional During the drying and transferring process of magnetic bead The UV lamp is on 10 taco 32 Nucleic Acid Automatic Extraction System 4 3 How to Reset taco 32 4 3 1 After the extraction is finished the yellow LED light will be on together with a long beep sound 4 3 2 Long press the DOOR button to stop the alarm and open the door 4 3 3 Take out the 96 Well Extraction Plate and Mixing Sleeves the extracted nucleic acids are ready for subsequent applications 4 3 4 To reset taco 32 long press the PAUSE RESET button 4 3 5 After a short beep sound taco 32 is reset and ready when the green LED lights are on Note Please transfer the extracted nucleic acids to a new tube immediately after extraction Quality of nucleic acids may decline otherwise Note The Reset button is only functional when Under PAUSE status Extraction is finished 4 4 When and How to Turn on UV Lamp 4 4 1 After the extraction is finished remove the 96 Well Extraction Plate and Mixing Sleeves and press the RESET button 4 4 2 Press the UV light switch at the rear of taco 32 to turn on UV lamp for sterilization of the space 4 4 3 Sterilization will continue for 30 minutes To manually turn
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