Olympus Student Microscopes CHA & CHB Instruction Manual
Contents
1. lt z lt Q a Z Q 3 lt z n Lu a Q oO 2 va Q LJ Q e 2 gt a 3 Scanned by J G McHone 8 Nov 09 for personal use only not for sale This instruction manual has been prepared for the Olympus Student Microscopes Models CHA and CHB It is recommended that you read the manual carefully in order to familiarize yourself fully with your microscope in order to obtain optimum performance from this Precision instrument IMPORTANT Observe the following points carefully m Operation 1 Always handle the microscope with the care it deserves and avoid abrupt motions or any impact 2 Avoid exposure of the microscope to direct sunlight dust and vibration 3 Only use the tension adjustment ring for altering the tension of the coarse adjustment knobs Do not twist the two coarse adjustment knobs in the opposite directions simultaneously as this will cause damage 4 Ascertain that the voltage selector switch on the base plate of the Model CHA is set to conform with the local mains voltage 5 Disconnect the line cord from the AC power outlet before fuse replacement Maintenance 1 Lenses must always be kept clean Fine dust on lens surfaces should be blown or wiped off by means of an air blower or a clean brush Carefully wipe off oil or finger Prints d2. Stand Objective E gt Mechanical stage Plain stage r Base Condenser Ill ASSEMBLY The picture below illustrates the sequential procedure of assembly The numbers indicate the assembly order of various components Take care at assembly to keep all glass surfaces clean and avoid scratching the lens surfaces Eyepiece cap ua Securely attach the mechanical amp Eyepiece stage to the plain stage with the clamping screws and tighten S them with a coin Observation tube Objective RS Observation tube clamping screw Mechanical stage Clamping screws Condenser A t Filter mount Line cord Pull down the locking knob of the lamp house cover Aligning the positioning dots on condenser mount and condenser insert the condenser into the mount from below and clamp with condenser clamping screw After opening the lamp house cover at the microscope base press the tungsten bulb socket against the lamp mount and rotate clockwise Tungsten bulb 20WCHB For CHB Gm For use of a halogen bulb insert its contact pins into the socket n case of the tungsten bulb its silver reflecting surface must be positioned pointing to the base plate Before use wipe off fingerprints or stains on the respective bulbs Halogen Lamp socket bulb CH LSHB For CHA O Tungsten bulb 6V10W For CHA E HE IV IDENTIFICATION AND FUNCTION OF
3. Voltage selector switch is not matched with the mains voltage Bulb does not light Bulb is burned out Set the switch to match the mains voltage Replace the bulb Loose electrical connection Secure the connection Reduced bulb life Voltage selector switch is not matched with the mains voltage Set the selector switch to match the mains voltage Bulb is not a standard one Use a standard bulb Bulb was over volted too long Reduce bulb voltage Focusing Coarse adjustment is too tight Tension adjustment ring is tighten ed too much Loosen the tension adjustment ring slightly User is trying to raise the stage pas sing over the upper focusing limit imposed by the engaged pre focus ing lever Unlock the pre focusing lever b Stage drops and the speci Tension adjustment is too loose ring Tighten the ring slightly men goes out of focus c Stage cannot be raised to the upper limit Pre focusing lever is engaged in lower than focusing position Unlock the pre focusing lever d Stage cannot be lowered to the lower limit of the working range Substage is lowered to much Raise the substage e Objective front lens touch Specimen is mounted on the stage es the specimen upside down 4 Binocular Observation Tube a Incomplete binocular vi sion Interpupillary distance is not cor rectly adj
4. When objectives are chang ed they are not parfocal Mechanical tube length is not cor rectly adjusted Adjust with the tube length adjust ment rings on the observation tube Light intensity does not increase although the volt age is raised Condenser is lowered excessively Raise the condenser Rheostat trimmer screw is not cor rectly adjusted Adjust it correctly 2 Electric System a Illuminator is too bright or too dark Voltage selector switch is not matched with the mains voltage for CHA Set the switch to match the mains voltage Mains voltage is too high or too low Adjust the mains voltage with a variable voltage transformer Rheostat trimmer screw is not cor rectly adjusted Adjust it correctly b Output voltage for the il luminator cannot be reg ulated Voltage selector switch is not matched with the mains voltage for CHA Set the switch to match the mains voltage Mains voltage is too low or too high Adjust the mains voltage with a variable voltage transformer Troubles Causes Remedies Light flickers and the in Mains voltage is unstable Use a voltage stabilizer tensity is unstable Filament of the bulb is likely to burn out Replace the bulb Loose electrical connection Secure the connection Fuse burns out too often Fuse is not a standard fuse Use a standard fuse
5. VARIOUS COMPONENTS Interpupillary distance scale Observation tube clamping screw Loosen the clamping screw slightly to rotate the observation tube as desired Mechanical tube length adjustment ring Rotate the ring to match your S interpupillary distance setting ob oe tained from the scale and make diopter adjustment Tension adjustment ring Coarse adjustment knob Specimen holder Fine adjustment knob Graduated in increments of 2 5H Low drive coaxial stage con trols Main switch Sliding control lever For continuously variable light intensity Rheostat trimmer screw After switching on if necessary rotate this screw with a coin until the bulb is dimly lit with the sliding control switch at minimum voltage position Fuse holder Grounding terminal Line voltage selector switch For CHA Set the switch to con form with the local mains voltage Automatic pre focusing lever Condenser height adjustment knob The condenser is generally used at top position For use with objectives 10X and lower power however it is recommended to lower the condenser properly to eliminate uneven field illumina tion Lamp mount Aperture iris diaphragm lever Filter mount Slip in type Filter mount Accepts 45mm diam filters Lamp house clamping knob The lamp house cover can be opened by pulling down the knob or closed
6. by pushing it up until it snaps in place Before pushing ascertain that the knob is positioned as shown in the picture right marked with circle V OPERATION Summary of Putting the Microscope in Operation 1 Match the line voltage selector switch to local mains voltage for CHA See page 5 Adjust the trimmer screw See page 7 Place a specimen slide on the stage See page 7 Switch on the light source Loosen the automatic pre focusing lever Oak wWON Coarse focus with a low power objective Make interpupillary and diopter adjustments See page 8 Swing in the desired objective Adjust light intensity ON Fine focus Lock the automatic pre focusing lever See page 8 12 Adjust the aperture iris diaphragm See page 9 Ss A Adjustment of Minimum Line Voltage CHA The minimum voltage required for the light source can be adjusted with the rheostat trimmer screw at the microscope base plate in accordance with the line voltage and frequency The built in rheostat incorporates a thyristor in its semi conductor circuit for the following advantages a Extremely fine adjustment of light intensity can be easily achieved b Flickering of the bulb filament is eliminated and the light intensity is stabilized c Increased life expectancy of the bulb For adjustment of the minimum line voltage ascertain that the voltage selector switch is set to conform with t
7. eposited on the lens surfaces with gauze moistened with a small amount of xylene alcohol or ether 2 Do not use organic solutions to wipe the surfaces of various components Plastic parts especially should be cleaned with a neutral detergent 3 Never disassemble the microscope for repair 4 The microscope should be stored in its container immediately after use If this is not possible it should be covered with the vinyl dust cover provided VI vil Scanned by J G McHone 8 Nov 09 for personal use only not for sale CONTENTS STANDARD EQUIPMENT VARIOUS COMPONENTS OF THE STUDENT MICROSCOPES MODELS CHA amp CHB ASSEMBLY IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS OPERATION A Adjustment of Minimum Line Voltage B Placing a Specimen Slide on the Stage C Interpupillary Distance and Diopter Adjustments D Tension Adjustment of Coarse Adjustment Knobs E Automatic Pre focusing Lever F Aperture Iris Diaphragm G Immersion Objectives OPTICAL DATA TROUBLESHOOTING How to put microscope fixing blocks provided with an optional wooden storage case The blocks should be put at the bottom of the wooden storage case in the following order P e Mi 1 Insert the screw with the flat washer into fixing block one of the two holes 8mmd as shown in the drawing Turn the screw to get into the block and tighten it with the spanner provided or a screwdriver Put the other block in
8. er u x 1 3 0 52 0 26 Spring Spring Eyepiece Remarks loaded loaded WF10X Total magnification 400X 1000X Field number 18 Focal depth 1 3 03 0 66 Field of view mm K 0 45 0 18 Immersion objective The resolving power is obtained if the objective is used with the fully opened aperture diaphragm e Working distance The distance from the specimen or cover glass to the nearest point of the objective Numerical aperture The N A represents a performance number which could be compared to the relative aperture of a camera lens The quantity of light which the objective receives from the object increases with the square of the performance number Resolving power The resolving power of a lens is measured by its ability to separate two points e Focal depth The distance between the upper and lower limits of sharpness in the image formed by an optical system As you stop down the aperture iris diaphragm the focal depth becomes deeper The larger the N A of the objective the shallower the focal depth Field number A number that represents the diameter in mm of the image of the field diaphragm that is formed by the lens in front of it Field of view diameter The actual size of the field of view in mm VII TROUBLESHOOTING Troubles Remedies 1 Optical System a Field of view is cut off or Nosepiece did not change properly Slightly rotate the nosepiece until illuminated ir
9. he local mains voltage and the sliding control lever 2 is positioned closest to you low voltage and then activate the main switch If the bulb is dimly lit the secondary voltage is correct If it is not lit at all rotate the rheostat trimmer screw 8 gradually with a coin until the bulb is dimly lit then push the sliding control lever forward in order to obtain optimum light intensity Fig 1 Fig 1 B Placing a Specimen Slide on the Stage NOTE 1 Cover glass Olympus objectives with an engraving 0 17 are corrected for use with cover glasses of 0 17mm thickness No 1 2 Specimen slide It is recommended to use specimen slides of 0 8mm to 1 5mm thickness However for use with the immersion darkfield condenser BH DCW optionally available a specimen slide between 0 8mm to 1 2mm thickness is preferable C Interpupillary Distance and Diopter Adjustments Binocular tube 1 Hold the knurled dovetail slides of the right and left eyepiece tubes with both hands and push the tubes together or pull them apart laterally which ever is required while looking through the eyepieces with both eyes until perfect binocular vision is obtained Fig 2 2 Rotate the tube length adjustment ring on the right eyepiece tube to match your interpupillary distance setting which you obtained from the scale Fig 2 3 Look at the image through the right eyepiece with your right eye and focus on the specimen w
10. ical aperture of the objective in use in order to achieve optimum objective performance as depth of focus image contrast and resolution However since microscopic specimens generally are low in contrast their image lacks contrast if the objective is used with its full numerical aperture Therefore it is often preferable to stop down the aperture diaphragm slightly more than indicated by the objective N A An aperture setting at 70 to 80 of the objective N A is recommended If necessary remove the eyepiece and looking at the exit pupil of the objective adjust the opening of the diaphragm 70 80 30 20 G Immersion Objectives To utilize the full numerical aperture of an immersion objective with engraving HI for homogeneous immersion the objective specimen and condenser are immersed in an immer sion oil Care should be taken to prevent oil bubbles from forming in the oil film between condenser specimen slide or objective After use carefully wipe off the immersion oil deposited on the lens surfaces with gauze moistened with xylene Never leave oil on lens surfaces after use as oil remnants will seriously impair the performance of the lens systems It is recommended to use Olympus immersion oil for immersion objectives VI OPTICAL DATA Objective Achromat Magnification 10X S40X S100X N A 0 25 0 65 1 30 W D mm 5 40 0 39 0 11 Focal length mm 15 98 4 31 1 81 Resolving pow
11. ith the coarse and fine adjustment knobs 4 Next looking at the image through the left eyepiece with your left eye rotate the tube length adjustment ring to focus on the specimen without using the coarse and fine adjustment knobs D Tension Adjustment of Coarse Adjustment Knobs A tension adjustment ring is provided next to the right hand coarse adjustment knob With this device the tension of the coarse adjustment is freely adjustable for either heavy or light movement depending on operator preference However do not loosen the tension adjust ment ring too much because this may cause the stage to drop or the fine adjustment knobs to slip The arrow mark indicates increase of the tension Be careful not to rotate the right and left coarse adjust ment knobs in the opposite directions simultaneously E Automatic Pre focusing Lever This lever D is provided to prevent possible contact between specimen and objective as well as to simplify coarse focusing The lever is locked after coarse focus has been accomplished This prevents further upward travel of the stage by means of the coarse adjustment knobs and automatically provides a limiting stop if the stage is lowered and then raised again The automatic pre focusing lever does not restrict fine focus ing Fig 4 Fig 4 F Aperture Iris Diaphragm An aperture iris diaphragm is provided on the condenser the opening of which can be adjusted to match with the numer
12. regularly it clicks into position Condenser is not correctly mounted Re insert the condenser all the way on the ring mount 10 Troubles Causes Remedies Dust or dirt is visible in the field of view Dust or dirt on the glass surface at the light exit on the base Dust on condenser top lens Remove dust or dirt Dirty specimen Dust on eyepiece Excessive image contrast Condenser is lowered excessively Raise the condenser Aperture iris diaphragm is stopped down excessively Open the diaphragm Resolution problems Image is not sharp Insufficient contrast Image details lack de finition Objective is not correctly position ed in the light path Slightly rotate the nosepiece until it clicks into position Dirt on objective front lens Clean the objective Immersion objective is used with out immersion oil Apply immersion oil Bubbles in the immersion oil Remove bubbles Olympus immersion oil is not used Use Olympus immersion oil Dirty specimen Dust on eyepieces and condenser top lens Field of view is partially out of focus Objective is not correctly position ed in the light path Slightly rotate the nosepiece until it clicks into position Specimen is not correctly position ed on the stage Place the specimen on the stage and secure it with the specimen holder or stage clips
13. the above order 10 10 I STANDARD EQUIPMENT CHA 213 W CHB 213 W Microscope stand with quadruple revolving nosepiece plain stage and in base illuminator 6V 10W halogen CHA F W Microscope stand with quadruple revolving nosepiece plain stage and in base illuminator 20W tungsten CHB F W Binocular observation tube inclined 45 BH B145 W Mechanical stage with coaxial right hand low drive controls CH MVR Abbe condenser CH CD Ach 10X Objectives Ach S40X spring loaded Ach S100X oil spring loaded Eyepieces BiWF 10X Lamp socket Halogen light source CH LSH CH LSHB Halogen bulbs 6V 10W HALCH Tungsten bulbs 20WCHB Spare fuses 0 5A for 100 110 120V or 0 3A for 220 240V Eyepiece caps Immersion oil bottled Vinyl dust cover m Optional Accessories Monocular tube inclined 45 Mechanical stage with coaxial left hand drive controls Tungsten bulb 6V 10W for CHA Wooden storage case CH MO45 CH MVL CH 6V 10W TP CHA B WSC Il VARIOUS COMPONENTS OF THE STUDENT MICROSCOPES MODELS CHA amp CHB These models are composed of various components and interchangeable accessories A variety of combinations standard or optional is available according to your requirements This is a picture of the Model CHA Eyepiece Observation tube Revolving nosepiece
14. usted Diopter adjustment is incomplete Reverse the specimen Correct the interpupillary distance Complete the diopter adjustment Right and left eyepieces are not matched Use a pair of matched eyepieces User is unaccustomed to binocular vision Prior to looking at the image of the specimen try to look at the entire field of view or look at a far away object before resuming microscopic observation
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