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Installing your UV-Visible Spectrophotometer

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1. Light Beam in Parallel Cell Walls Light Beam out Light Beam in Non parallel Cell Walls Light Beam out Figure 6 Shift of the instrument light beam due to non parallel cell walls 38 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Good Measurement Practices 3 Apertured Cells or Cuvettes In applications where sample volume is limited apertwred or microcells are used The width of these cells is reduced to reduce the volume and the blank part of the cell must be blackened to avoid unwanted transmission and reflection through the side walls If the side walls are not blackened the result will be poor photometric accuracy and if different concentrations are measured poor linearity The disadvantage of apertured and microcells is that part of the light beam is blocked Not all the light passes through the sample and there can be some loss in sensitivity See Figure 7 for recommended cells and Figure 8 for cells you should not use with the instrument i S LAA Quartz cells Quartz cells with black apertures lt gt Figure 7 Recommended cells CAUTION Quartz cells with black apertures smaller than 2 mm when used with a multicell transport can lead to measurements of poor reproducibility Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 39 3 Good Measurement Practices Cs
2. NI Quartz cells with transparent apertures Fluorescence cells Plastic cells Figure 8 Cells you should not use with the instrument Flow Cells We recommend a sipper system with a flow cell for obtaining high precision measurements Using a flow cell eliminates the necessity of moving the cell between blank measurement and sample measurement Also the cell can be rinsed thoroughly with the solution to be measured The design of the flow cell should minimize entrapment of bubbles and flow channeling to provide the most reliable results 40 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Good Measurement Practices 3 Handling and Maintaining Cells or Cuvettes Passivating New Cells When filling a non passivated new cell with your sample you will observe that air bubbles stick on the windows of your cell To prevent the formation of sticky bubbles rinse the cell with cleaning and passivating fluid part number 5062 8529 The cleaning procedure is described on the label of the cleaning fluid container Cleaning Cells The fats in fingerprints are significant absorbers in the UV region and if left on optical surfaces can cause erroneous results Wipe off all fingerprints and contaminants before using a sample cell Use only high quality lens tissues part number 9300 0761 and never dry the inside of a cell with lens tissues Dry the inside of the cell with pressurized oil free air that prevent
3. Cell clamps Position cell here D 45 Clamp screws Figure 18 Positioning the cell stops for a 1 cm sample cell Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 57 4 Installing Accessories 2 If your sample cell is not one of the standard lengths position the cell stops as for a long path sample cell i e move all cell stops in Figure 18 to the left 3 Because the light beam is collimated absolute positioning of the sample cell is not important however consistent cell positioning gives more consistent results The cell stops make it easier to consistently position your sample cells 4 Install the sample cell between the clamps Slide it against the cell stop If your sample cell is not one of the standard lengths position it so the clamps are gripping the mid point of the cell 5 Allow the clamps to close against the sample cell see Figure 19 Tighten the four screws that hold the sample cell in place Figure 19 Installed 10 cm cylindrical cell in the long path cell holder 58 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing Accessories 4 Optical Filter Wheel To achieve optimum measurement conditions optical filters may be necessary for spectral measurements of photosensitive samples Agilent Technologies offers an optical filter wheel assembly 08451 60302 see Figure 20 which can be mounted on the standard
4. 9 Exit the Configuration Editor by selecting File gt Exit 10 Reboot the PC and power cycle the instrument 11 Wait until the front panel light on the instrument turns green Then start the Agilent ChemStation to check if the software communicates with the spectrophotometer In case the red error message line Power Fail shows up refer either to the following troubleshooting section or repeat the installation procedure 130 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Troubleshooting LAN Communication Power failure reported on the Agilent ChemStation If your Agilent ChemStation can not connect to the spectrophotometer using LAN communication please perform the following troubleshooting steps Verify that basic communication is possible Use the ping command to verify that the IP address is operational 1 From the Start menu in the taskbar select Start gt Run In the Open field type cmd exe and press Enter Type ping lt IP number gt and press Enter to verify communication of the LAN Interface Card and the LAN interface in your PC lt IP number gt needs to be replaced by the appropriate IP address e g 10 10 10 101 or host name The command ping will send a request for reply to the IP address bypassing part of the Windows TCP IP settings A successful ping would look like this Reply from 10 10 10 101 bytes 32 time lt 10ms
5. Agilent Technologies 823574 has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing STOP instalation Hardware Installation warning 11 Click Finish to complete the hardware setup Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for Agilent Technologies 823574 Click Finish to close the wizard Cancel Figure 47 HardwareWizard completion Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide The hardware installation warning may appear If so click Continue Anyway 97 6 GPIB Communication Connections and Configuration Configuring the Agilent 82357 USB GPIB Interface 12 Start the IO Config Utility by clicking in the IO Icon METTE and choose Agilent Connection Expert 13 Uncheck Show this window at startup and click Close Welcome Screen The Agilent Connection Expert Main Window is displayed see Figure 48 E Agilent Connection Expert File Edit view I O Configuration Tools Reftesh All WP Undo IZ Interactive 10 MB Add Instrument W9 Add Interface XC D
6. Description Transmission Control Protocol Intemet Protocol The default wide area network protocol that provides communication across diverse interconnected networks SSSA OK al Cancel Figure 56 Local Area Connection Properties 2 In the Local Area Connection Properties dialog see Figure 56 click Configure to make sure the device is working properly If the device is not working properly or no network interface card is configured please refer to the hardware manual of your network card and the PC for how to install and configure a network interface card Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 3 In the This connection uses the following items section of this dialog make sure the Internet Protocol Version 4 TCP IPv4 is available and checked If the TCP IP protocol is not installed use the Install button select Protocol from the list of network components to install and press Add From the Manufacturers select Microsoft and from the Network Protocol select the Internet Protocol Version 4 TCP IPv4 and press OK If required reboot your computer 4 Select the Internet Protocol Version 4 TCP IPv4 and click Properties If the PC is connected to your companies LAN do not change any setting Note down the Subnet Mask if available Ask your responsible
7. Setup will install Agilent UY ChemStation Rev B 02 01 in the following folder To install to this folder click Next To install to a different folder click Browse and select another folder r Destination Folder C Chem32 Browse lt Back 6 Select the additional modules you want to install and click Next InstallShield Wizard Select Components Choose the components Setup will install Select the components you want to install and clear the components you do not want to install 9 G111648 G112244 Advanced Add On Description 7 G111744 G1123AA Biochemical Add On This selection will install all 7 G111844 G112644 Dissolution Add On ee files for ECM G181344 Security Pack Add On J v Space Required on C 105724 K Space Available on C 15793568 K lt Back Cancel Note that the Security Pack Add On and the ECM Add On are mutually exclusive One or the other can be installed but not both 7 Enter the License Registration Number for each selected module in the entry line and click Add License When all required licenses are added Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing the UV Visible Software 5 press NEXT InstallShield Wizard Enter License information Please enter licenses for all selected Instruments and Add Ons Product Remove License G111544 G112144 UV Vis General Purpose ChemStation G111644 G11
8. and so on Avoid star configurations connecting all of the devices to a central point Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 The Agilent 82357A B USB to GPIB interface has an integrated 2 5 m cable and an GPIB connector In a single instrument configuration no additional GPIB cable is required WARNING According to the IEEE 488 specification for the General Purpose Instrument Bus GPIB the bus is not designed for dynamic configuration Cycling power on an GPIB instrument that is connected to the bus while other GPIB instruments are actively communicating to the GPIB controller can induce electrical spikes that may potentially corrupt the GPIB protocol In extreme cases this may require cycling power on all instruments including the GPIB controller that is typically the Agilent ChemStation Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 81 6 GPIB Communication Connections and Configuration Installation of the Agilent GPIB Communication Library Installing New 10 Libraries 1 Turn the Computer on and log on Local administrator rights are required to perform the setup and configuration of the Agilent GPIB 1 0 Library 2 Run Setup exe in the GPIB directory of your UV Visible ChemStation CD ROM Follow the instructions in the installation Wizard This will install the Agilent IO Libraries Suite on your computer upda
9. light when the instrument is turned on When the line power switch stands out and the green light is off the instrument is turned off On the front panel of the spectrophotometer is an indicator which will display different colors depending of the actual condition of the instrument e Green the instrument is ready to measure e Green blinking the instrument is measuring e Yellow the instrument is in busy state for example turning one of the lamps on or if both lamps are switched off e Red Error condition that is the spectrophotometer does not pass one of the self tests which are run when the spectrophotometer is turned on or an error occurred during operation In this case the UV Visible operating software gives a detailed error message and possible explanations are in the online help system Additionally the Reference Manual gives information about troubleshooting Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and Start Up 2 e Red blinking Error condition of the spectrophotometer processor system Because in this case there is no communication with the computer there will be no error message The online help system and the Reference Manual gives more information about troubleshooting The four pushbuttons on the front panel cause the following actions to be performed and the resulting data being sent to the computer e blank the instrument takes a blank measurement that is a referen
10. such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 137 A Safety Information e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF 138 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Safety Information A Other Precautions Use of the spectrometer system and accessories may involve materials solvents and solutions which are flammable corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards chemical burn hazards fire hazards toxicity and other hazards that can result in death serious personal injury or damage to equipment Apply all necessary precautions including use of lab coats safety goggles and other appropriate forms of personal protection All wastes should be disposed of in accordance with local regulatory requirements Protective Earth Shock Hazard Connecting the Agilent Cary 8454 UV Vis to a power source that is not equipped with a protective earth contact creates s shock hazard for the operator and can damage the instrument Always
11. where the specific gravities or miscibilities of the solvent and analyte are quite different Again stirring is a way to prevent this kind of problem Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 45 3 46 Good Measurement Practices In an unstirred cell it is sometimes possible to observe local photodegradation of sensitive analytes Because the actual volume of the sample in the light path is very small stirring the sample will reduce the time any given analyte molecule is in the light path This minimizes the photodegradation and increases homogeneity Using a flow cell with continuous flow can yield similar results Checklist for Best Results Cell V Cell is made of quartz or glass V Apertured cells have black sides V Apertured cells have an aperture greater than or equal to 3 mm Y Cell windows are free of fingerprints and other contamination V Flow cell used instead of an apertured standard cell Measurements Y Solution in cell is free of floating particles V Both solution in cell and cell walls are free of bubbles Y Solution in cell is mixed homogeneously V Blank measured on same solvent as sample V Blank measurement shows a flat baseline Figure 11 and Figure 12 show a good and a poor baseline Y Cell orientation of blank and sample measurements is the same Y Ideally the cell is not removed between the measurement which means the cell is filled rinsed using a pipette or a flow ce
12. Configuration menu select that element under Screen Elements then select a color from those shown below in the Standard Colors or Custom Colors list box Your changes will appear in the Screen Elements list box You can create custom colors and add these use the Add Custom Colors button and follow the instructions available with the Help Select and configure the GPIB interface For GPIB communication in the configuration dialog for the GPIB Card field hp82341 must be selected Click OK to save changes and return to the Configuration Editor display If you want to add new instruments Choose Add New Instrument from the Add Delete menu to add a new instrument window to the Configuration Editor screen Follow the above procedure to configure the instrument Choose Delete Instrument from the Add Delete menu to remove an instrument from the configuration Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing the UV Visible Software 5 7 Choose Save from the File menu to save your configuration 8 Choose Exit from the File menu to close the Configuration Editor 9 To set parameters for the accessories you have installed and configured refer to the Help in the UV Visible ChemStation software Autosamplers If you have an Agilent XY autosampler your Agilent ChemStation software requires a special driver and a cable See the readme txt for further information Agilent Cary 8454 UV Visible Spectroscopy Sy
13. IT consultant for this mask if not available and request a fixed IP address for your instrument if you want to use the existing LAN for the instrument connection For further details please see Connecting the Spectrophotometer to the PC Internet Protocol Version 4 TCP IP v4 Properties Y mE General You can get IP settings assigned automatically if your network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings Obtain an IP address automatically Use the following IP address IP address 10 10 10 1 Subnet mask 255 255 255 J Default gateway Obtain DNS server address automatically Use the following DNS server addresses Preferred DNS server Alternate DNS server Figure 57 Internet Protocol Version 4 TCP IPv4 Properties Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 113 7 LAN Communication Installation Connection and Configuration 5 In the Internet Protocol Version 4 TCP IPv4 Properties dialog see Figure 57 select the option Use the following IP address and enter your computer s IP Address and Subnet Mask a Ifthe system is on an isolated LAN the following defaults can be used 192 168 254 10 for the PC IP address and 255 255 255 0 for the subnet mask note to use these defaults DIP switches 5 and 6 on the LAN card must be ON Gateway DNS and WINS IP addresses do not need to be configur
14. Installation Guide 55 4 Installing Accessories Long Path Cell Holder 56 The Agilent 89076A long path cell holder is a sample cell holder which can be adjusted to hold most cylindrical and rectangular sample cells with path lengths of up to ten centimeters It uses the same mounting system as the other sample cell holders Cell stops Figure 17 Long path cell holder The long path cell holder includes built in cell stops for accurate positioning of 1 2 5 and 10 cm sample cells The optical filter wheel 08451 60302 can be mounted on the long path cell holder in the same way it is mounted on other cell holders see Optical Filter Wheel on page 59 for details However instead of a screwdriver a 2 5 mm hex wrench is required to install the optical filter wheel on the long path cell holder Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing Accessories 4 Installing the Long Path Cell Holder 1 Loosen the hold down screws and remove the current sample holder from the sample compartment 2 Orient the long path cell holder so that the clamps that hold the sample cell in place are on the right Lower the cell holder onto the sample compartment mounting guides 3 Tighten the hold down screws Installing a Sample Cell 1 Position the cell stops according to the path length of the sample cell you will be using This example shows a 1 cm sample cell Cell stops
15. O base address are required Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 The Agilent 82357A B USB to GPIB interface is connected to and powered by one of your PC s USB connectors The Agilent 82341C ISA GPIB interface is not supported by Agilent ChemStations revision B 04 xx or higher Installing an GPIB Interface The Agilent 82357A B USB to GPIB interface is connected to one of your PC s USB connectors This does not require opening your PC Since Peripheral Component Interconnect PCI interface boards can be installed in several different computers the following instructions are general If you have difficulties during the installation consult your computer manual or dealer WARNING Turn off and unplug your computer and all attached electrical devices before removing any covers 1 Turn off and unplug your computer then remove the computer s cover s 2 Select the appropriate empty slot for your GPIB card The 82350A B plugs into any PCI slot It is best not to place the GPIB board next to a graphics interface As the Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 79 80 GPIB Communication Connections and Configuration GPIB connector is wider than a typical PC slot you may have to leave the slot s adjacent to the GPIB interface empty 3 Loosen the mounting screw and remove the selected empty slot s rear
16. TTL 128 If the message request timed out is displayed the IP address can not be reached by the ping command If the ping request was answered successfully by the instrument you need to verify that your Windows TCP IP settings are correct for the selected network especially the subnet mask and gateway settings should be checked Identify by host name If you identify the UV Visible system by host name ensure that the used host name and IP address are correctly setup in the DNS server Try using the IP address in the Configuration Editor Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 131 7 LAN Communication Installation Connection and Configuration Verify that the LAN Interface card is correctly configured To ensure that all parameters of the LAN interface card are set correctly use the Agilent BootP Service program from the Agilent UV Visible ChemStation CD ROM and disable any other means that configure the LAN interface card of the instrument Install the bootp service as described in section Using the Agilent BootP Service on page 118 and configure it for the MAC address of the LAN interface card DHCP server Make sure that no DHCP server runs in the same subnet as the instrument s as these servers also may respond to a bootp request and do not set the buffer handling of the LAN interface card correctly i e T145 64 T146 01 T147 01 They also may assign a different IP address to the instrument
17. The RS 232 connector may be used to control the spectrophotometer from a computer through RS 232 connection using appropriate software for future use This connector needs to be activated by the configuration switch module next to the GPIB connector The software needs the appropriate drivers to support this communication See your software documentation for further information The GPIB connector is used to connect the spectrophotometer with a computer The 8 bit configuration switch module next to the GPIB connector determines the GPIB address of your spectrophotometer The switches are preset to a default address recognized by the operating software from Agilent Technologies The MIO slot for the Lan interface The accessory board slot is reserved for future use The power input socket accepts a line voltage of 100 240 V AC with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA There is no voltage selector on your spectrophotometer because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents that the spectrophotometer cover is taken off when line power is still connected On the right side of the instrument there is a door for exchanging the lamps Behind this plastic door there is another sheet metal door Two independent safety light switches are implemented
18. against packing list For more information see Unpacking Your Spectrophotometer on page 22 V Install your UV Visible spectrophotometer Connect line power to the rear Connect the waste tubing underneath the sample pan For more information see Installing your UV Visible Spectrophotometer on page 23 V Install your computer and printer Connect the keyboard and mouse Connect the monitor to line power and to the computer Connect the printer to line power and to the computer Connect the computer to the spectrophotometer with the GPIB or LAN cable For more information see Installing Your Computer on page 29 and Chapter 7 LAN Communication Installation Connection and Configuration Y Switch on the spectrophotometer make sure the sample area is clear wait 2 minutes for the lamps to ignite When you are ready to operate your spectrophotometer switch on the computer and printer For more information see Starting the Spectrophotometer on page 30 12 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Fast Installation 1 Installing Non Preconfigured Agilent Cary 8454 UV Visible Spectrophotometer Systems You must install and configure the software In addition you may need to install and configure an instrument communication interface such as the GPIB PCI interface card V Ensure you have space and an appropriate power supply For more information see Site Requirements
19. defaults global defaults sm 255 255 255 0 gw lt gateway address ask your system administrator gt 8454 lab UV Vis ht 1 ha 0030D30D3277 ip lt ip address ask your system administrator gt sm 255 255 255 0 gw lt gateway address ask your system administrator gt vm auto hn bs auto T145 64 T146 01 T147 01 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 125 7 LAN Communication Installation Connection and Configuration The entries are described below 8454 lab Comment line starting the configuration set for an Agilent Cary 8454 UV Visible spectrophotometer A comment is preceded with all characters after the on the same line are ignored by the BootP Service UV Vis Host name of the instrument The host name communication can only be used if a DNS server Domain name server is correctly set up for the name or a corresponding entry in the HOSTS file exists windows system32 drivers etc hosts ha 0030D30D3277 This is the hardware or MAC address of the G1369C LAN card It is usually printed on a label attached to the card If not see section Determine your MAC Address on page 121 ip lt IP address ask your system administrator gt This is the IP address that will be assigned to the card after the BootP request This IP address must be configured using the Agilent ChemStation Configuration Editor sm 255 255 255 0 This is the subnetmask assi
20. for Your Spectrophotometer on page 19 V Unpack the system check for damage and contents against packing list For more information see Unpacking Your Spectrophotometer on page 22 Y Install your UV Visible spectrophotometer Connect the line power to the rear Connect the waste tubing underneath the sample pan For more information see Installing your UV Visible Spectrophotometer on page 23 V If your computer and printer are not supplied by Agilent ensure that the requirements for proper operation of the software are met For more information see Minimum PC Requirements for Agilent ChemStation on page 64 V Install the GPIB interface board in your computer For more information see Installing an GPIB Interface on page 79 V Install your computer and printer Connect the keyboard and mouse Connect the monitor to the power supply and to the computer Connect the printer to the power supply and to the computer Connect the computer to the spectrophotometer with the GPIB cable Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 13 1 14 Fast Installation For more information see Installing Your Computer on page 29 V Switch on the spectrophotometer make sure sample area is clear and wait two minutes for the lamps to ignite When you are ready to operate your spectrophotometer switch on the computer and printer For more information see Starting the Spec
21. for the spectrophotometer The cut off wavelength of the filter you choose should be low enough that it does not eliminate important spectral information but high enough that it blocks the light that could degrade your sample If you use a filter with your samples you must use the same filter when you make your blank measurement Turning the Dj Lamp off The short wavelength radiation leading to photodegradation comes from the light of the Dy Lamp For application where readings are taken at wavelengths above 400 nm the Dy Lamp can be turned off The light intensity supplied by the Tungsten lamp is sufficient for a good signal to noise ratio over the wavelength range 400 nm 1100 nm When using cells with small apertures check the signal to noise ratio by making sample measurements under conditions of your application Stirring and Temperature Control Solution homogeneity can be a problem especially for viscous solutions There are cases where due to convection induced gradients rapid absorbance changes may give irreproducible data These changes can be observed spectroscopically by taking measurements with short integration times To minimize convection effects keep the temperature of your sample the same as the cell holder or environmental temperature Problems like these can also be minimized by using a thermostattable cell holder and or a stirring module A similar effect can occur in cases of incomplete mixing This is especially true
22. or Remove Programs dialog 2 In the Add or Remove Programs dialog select Agilent IO Libraries Suite and click Change Remove After a few seconds of preparation the Agilent IO Libraries Suite InstallShield Wizard is displayed see Figure 52 Agilent 10 Libraries Suite 15 0 InstallShield Wizard Modify repair or remove Agilent I0 Libraries Suite 15 0 Full version number 15 0 10528 0 An identical version of Agilent IO Libraries Suite 15 0 is already installed on your PC You may modify repair or remove it Click one of the options below O Modify amp Select new program features to add or select currently installed features to remove OFepait iF Reinstall all program features installed by the previous setup O Remove E Remove all installed features Figure 52 Agilent 10 Libraries Suite InstallShield Wizard Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 103 6 GPIB Communication Connections and Configuration Troubleshooting 3 Select the Repair option and click Next Wait until the repair process is finished 4 Click Finish to complete the repair see Figure 53 Agilent 10 Libraries Suite 15 0 InstallShield Wizard Maintenance Complete InstallShield Wizard has finished performing maintenance operations on Agilent IO Libraries Suite 15 0 Figure 53 Agilent 10 Libraries Suite maintenance complete 104 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GP
23. plate 4 Holding the board by its edges insert the board into its slot Make certain that the board s edge connector is fully seated Lock the board into place with the mounting screw 5 Replace the computer s cover s Plug in and restart the computer GPIB Cabling When connecting GPIB devices together there are several basic rules that should be observed 1 Whenever possible turn off and unplug the computer and all attached devices before the GPIB cables are installed 2 Before connecting any analytical instrument to an GPIB cable consult the documentation supplied with each device and determine its GPIB address No two devices including the PC connected to a GPIB interface may have the same address Alter their addresses as necessary to avoid any duplication Write down each GPIB address This information will be needed later 3 Try to use short GPIB cables that are two meters long or less 4 GPIB cable 0 5 m 10833D GPIB cable 1 0 m 10833A GPIB cable 2 0 m 10833B GPIB cable 4 0 m 10833C 5 Connect one end of an GPIB cable to the computer s GPIB connector or use the USB GPIB converter interface to connect to the first instrument 6 Be certain that you properly tighten all GPIB connectors A poor connection causes errors which are difficult to diagnose 7 Connect GPIB devices in a chain A chain occurs when an GPIB device is connected to the next GPIB device and it is in turn connected to the next
24. solvent s absorbance characteristics over the wavelengths of interest its suitability as a solvent for the analyte and on experimental conditions Table 1 lists common solvents and the lower limit of their useful wavelength range Table 1 Lower limit of UV transmission for some common solvents Lower Limit Solvent 180 195 nm 200 210 nm 210 220 nm 245 260 nm 265 275 nm 280 290 nm Above 300 nm Sulfuric acid 96 Water Acetonitrile Cyclopentane n Hexane Glycerol 2 2 4 Trimethylpentane Methanol n Butyl alcohol Isopropyl alcohol Cyclohexane Ethyl ether Chloroform Ethyl acetate Methyl formate Carbon tetrachloride Dimethyl sulfoxide Dimethyl formamide Acetic acid Benzene Toluene m Xylene Pyridine Acetone Carbon disulfide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 43 3 Good Measurement Practices Many of the solvents in Table 1 are hazardous Be sure you fully understand their properties before using them When using volatile solvents such as acetone or methylene chloride make sure that the sample cell is stoppered Evaporation of a solvent can change the solute concentration or cause solution noise due to solute convection currents Both of these will affect the accuracy of your measurements We also recommend stirring and temperature control when you use volatile solvents When using water as solvent we recommend using UV grade or HPLC grade water
25. the configuration procedure If the PC has been rebooted after installing the Agilent BootP Service the Agilent BootP Service is started automatically during the next startup of the system To change BootP settings the service must be stopped the changes made then restarted The details are documented within this section Determine your MAC Address Determine the MAC address of the LAN card installed The number is engraved in the board Write down the MAC address before installing the card into the module To remove the card from the system turn the module off before removing the LAN card Read the MAC address from the label Reinstall the card and turn on the module again Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 121 7 122 LAN Communication Installation Connection and Configuration Using the Launch Manager 1 To add the instrument to the network go to Start gt Programs gt Agilent BootP Service gt Edit BootP Settings The BootP Settings screen appears 2 Uncheck Do you want to log BootP requests 3 Click Launch Manager The BootP Manager screen appears Hardware Address HostName IP Address Subnet Mask Gatewa 0030D 3003277 UV Vis 10 10 10 101 8454 lab 255 255 255 0 10 10 10 1 Modify Delete Exit Manager Figure 60 Configured interfaces view of the Agilent BootP Service 4 Click Add The Add BootP Entry screen appears 5 Make the following entries for your instrumen
26. use small fonts whereas for a monitor with a 1024x768 or higher resolution you may select a larger font The font size is available with the control panels s Display settings In the Display properties dialog box use the Appearance tab for the adjustment 72 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing the UV Visible Software 5 Configuring Your System The setup program configures the system automatically for your UV Visible spectrophotometer as Instrument 1 with an GPIB address of 25 If you wish to use LAN communication a different GPIB address set up an additional spectrophotometer or set up an temperature control accessory you must configure the system To configure your system 1 In the Agilent ChemStation group start the Agilent UV Visible Configuration Editor 2 Choose Instruments from the Configure menu The Instrument Type box lists the instrument types available on your Agilent ChemStation Select one to begin your configuration Enter the name you want to call the instrument in the Instrument Name box The Agilent ChemStation will use this name to identify the instrument Select Normal under Initial Screen Window Size to have your instrument session window appear as a full screen but with an open area across the bottom for icons If you select Icon or Full Screen the instrument session window will start as an icon or as a full screen Click OK to enter your selections and t
27. your instrument Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 19 2 Installation and Start Up Power cords Different power cords are offered as options with the spectrophotometer The female end of each of the power cords is identical This connects to the power input socket at the rear of the spectrophotometer The male end of each of the power cords is different and designed to match the wall socket of a particular country or region Do not replace the mains power cord with one that is inadequately rated When replacing the mains power cord use only a mains power cord with a rating equivalent to the one supplied with the instrument See the instrument installation guide for detailed mains power cord rating information Always operate your instrument from a power outlet which has a ground connection Always use the power cord designed for your region 20 Space Do not position the equipment so that it is difficult to operate the disconnecting device The spectrophotometer is 34 4 cm 13 5 inches wide 56 0 cm 22 0 inches deep and 18 5 cm 7 3 inches high see Figure 1 on page 24 It weighs 14 kg 30 86 lbs and will fit on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side to allow for the circulation of air for cooling and approximately 8 cm 3 1 inches in the rear for electrical connections The computer including the keyboard is appro
28. 0 GPIB Interface the Agilent IO Libraries Suite must be installed See Installation of the Agilent GPIB Communication Library on page 82 for details e The 82350 PCI GPIB interface must be installed in your PC See Installing an GPIB Interface on page 79 for hints If the Found New Hardware Wizard appears and the Agilent I0 Libraries Suite is not installed press the Cancel button Local administrator rights are required to configure your interface 86 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Configuring the Agilent 82350 GPIB Interface 1 Boot your PC a tool tip will notify you about new hardware and the Found New Hardware Wizard will start Select Option 2 and click Next see Figure 32 Found New Hardware Wizard Welcome to the Found New Hardware Wizard This wizard helps you install software for Agilent Technologies 82350 PCI GPIB If your hardware came with an installation CD Q or floppy disk insert it now What do you want the wizard to do A Install from a list or specific location Advanced Click Next to continue lt Back Cancel Figure 32 Found New Hardware Wizard Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 87 6 GPIB Communication Connections and Configuration Configuring the Agilent 82350 GPIB Interface 2 Select Option 2 and click Next see Figure 33 Found New H
29. 2 Regulatory Information 143 CECompliance 143 Electromagnetic Compatibility 143 Agilent Technologies on the Internet 145 Index 147 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 9 Contents 10 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 1 Fast Installation Installing Preconfigured Agilent Cary 8454 UV Visible Systems 12 Installing Non Preconfigured Agilent Cary 8454 UV Visible Spectrophotometer Systems 13 Installing Software Only 15 Installation is straightforward but if you have difficulties or need more information references to detailed installation information are given Use this chapter if you are e installing a preconfigured UV Visible spectroscopy system from Agilent including spectrophotometer computer and software e installing a UV Visible spectroscopy system not purchased as a preconfigured system or e installing software only Phe Agilent Technologies 1 Fast Installation Installing Preconfigured Agilent Cary 8454 UV Visible Systems If you have purchased one of these systems the software is pre installed and preconfigured You need only install and switch on the hardware V Ensure you have space and an appropriate power supply For more information see Site Requirements for Your Spectrophotometer on page 19 V Unpack the system check for damage and contents
30. 2244 Advanced Add On G111844 G11264A Dissolution Add On G181344 Security Pack Add On The applicable product and license numbers are printed on your Software Certificate and Registration Packet see the example license registration label SOFTWARE REGISTRATION LABEL FOR PRODUCT NUMBER G1115AA REVISION CODE A 08 01 REGISTRATION AA MMIC SESE Figure 23 Example license registration label Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 71 5 Installing the UV Visible Software 8 The configuration editor will open automatically as part of setup Adjust the configuration if desired the default is for GPIB communication from the PC to the spectrophotometer Save the configuration and exit the configuration editor Setup will then continue 9 Click Finish to complete the setup and reboot your system InstallShield Wizard orere B InstallShield Wizard Complete The InstallShield Wizard has successfully installed Agilent UV ChemStation Rev B 02 01 Before you can use the program you must restart your computer No will restart my computer later Remove any disks from their drives and then click Finish to complete setup If you selected the G3770AA or G5182AA ECM access module establish your ECM access using Internet Explorer before you first launch UV Visible ChemStation Monitor Configuration If you have a monitor with a 800x600 pixel resolution
31. 8 14 G1115 90053 EE Agilent Technologies
32. Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide oe Agilent Technologies Notices Agilent Technologies Inc 2002 2003 2008 2011 2013 2014 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G1115 90053 Edition 08 14 Agilent Technologies Australia M Pty Ltd 679 Springvale Road Mulgrave Victoria Australia 3170 Research Use Only This product may be used as a component of an in vitro diagnostic system if the sys tem is registered with the appropriate authorities and complies with the relevant regulations Otherwise it is intended only for general laboratory use Software Revision This handbook is for B 05 xx revisions of the Agilent ChemStation software where xx is a number from 00 through 99 and refers to minor revi sions of the software that do not affect the technical accuracy of this handbook Microsoft is a U S registered trade mark of Microsoft Corporation Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties eit
33. C with a yellow warning symbol with an exclamation mark Use the File menu s Exit command and in the upcoming dialog click Reboot Now After the reboot your GPIB library installation is complete Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 91 6 GPIB Communication Connections and Configuration Configuring the Agilent 82357 USB GPIB Interface Configuring the Agilent 82357 USB GPIB Interface 92 e To configure your 82357 USB GPIB Interface the Agilent IO Libraries Suite must be installed See Installation of the Agilent GPIB Communication Library on page 82 for details e A USB 1 0 or 2 0 connection on your PC must be available Local administrator rights are required to configure your interface 1 Plug the 82357A B USB GPIB Interface into the GPIB connector of the instrument s GPIB connector If a GPIB cable is used to interconnect multiple modules e g 89090A Peltier Temperature controller the USB Interface device must also be plugged into the GPIB connector see Figure 39 taaan Tna ee Figure 39 82357 USB GPIB interface on a spectrophotometer Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Configuring the Agilent 82357 USB GPIB Interface 2 Connect the USB connector of the 82357 USB GPIB interface into a USB port of your Computer The hardware wizard detects a USB device and the Found New Hardware Wiz
34. IB Communication Connections and Configuration 6 Table5 GPIB address switch settings addresses 0 through 15 Address 1 2 3 4 5 6 7 8 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 1 0 3 0 0 0 0 0 0 1 1 4 0 0 0 0 0 1 0 0 5 0 0 0 0 0 1 0 1 6 0 0 0 0 0 1 1 0 7 0 0 0 0 0 1 1 1 8 0 0 0 0 1 0 0 0 9 0 0 0 0 1 0 0 1 10 0 0 0 0 1 0 1 0 11 0 0 0 0 1 0 1 1 12 0 0 0 0 1 1 0 0 13 0 0 0 0 1 1 0 1 14 0 0 0 0 1 1 1 0 15 0 0 0 0 1 1 1 1 WARNING Switches 1 and 2 are used for factory firmware settings Do not change these switches Any changes could result in instrument failure To recover set switches 1 and 2 to position 0 and power cycle the instrument For more detailed information see the Service Manual Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 109 110 GPIB Communication Connections and Configuration Table 6 GPIB address switch settings addresses 16 through 31 Address 1 2 3 4 5 6 7 8 16 0 0 0 1 0 0 0 0 17 0 0 0 1 0 0 0 1 18 0 0 0 1 0 0 1 0 19 0 0 0 1 0 0 1 1 20 0 0 0 1 0 1 0 0 21 0 0 0 1 0 1 0 1 22 0 0 0 1 0 1 1 0 23 0 0 0 1 0 1 1 1 24 0 0 0 1 1 0 0 0 25 0 0 0 1 1 0 0 1 26 0 0 0 1 1 0 1 0 27 0 0 0 1 1 0 1 1 28 0 0 0 1 1 1 0 0 29 0 0 0 1 1 1 0 1 30 0 0 0 1 1 1 1 0 31 0 0 0 1 1 1 1 1 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 7 LAN Communication Installation Connect
35. IB Communication Connections and Configuration 6 Problems with Plug and Play option GPIB cards Operation systems using Plug and Play may show problems during the installation of the appropriate drivers for the GPIB PCI cards In order to overcome plug and play related problems please follow the steps to install the card successfully 1 If already installed un install the Agilent IO Libraries from Control Panel Add Remove Programs 2 Remove the GBIB Interfaces if installed listed under the Device Manager and shut down the computer Control Panel double click System Hardware Tab Device Manager GPIB Interfaces 3 Physically remove the GPIB card from the computers PCI Interface 4 Restart the computer and log on to the system Install the Agilent IO Libraries Suite Do not configure the card at this time and shut down the computer 5 Install the GPBI card again and turn on the computer the new hardware found wizard will indicate that a PCI card is found Select the second choice to search from a list of known devices and there select the option for Agilent Technologies since the IO Libraries are already installed 6 Ifthe new hardware wizard does not run check the device manager to verify that the GPIB interface is listed and installed correctly A Device Manager Of x Eile Action View Help e merala By TK UV XP2 amp 3 Computer B s Disk drives Ej a Display adapters 2 DYD CD ROM drives H Floppy disk contr
36. Instrument Bus and GPIB General Purpose Interface Bus GPIB communication can be used to control Analytical instruments by means of a controller The Agilent Cary 8454 UV Visible spectrophotometer and the optional Peltier Temperature Station 89090A have build in GPIB interfaces PCs are the common controllers today Most PCs do not offer a GPIB interface by default However such interfaces can be added Two different types of interfaces are supported by the UV Visible ChemStation software the PCI interface board Agilent 82350A B and the USB to GPIB converter interface 82357A B To configure the GPIB interface on the PC and provide the interface driver for the PC s operating system the Agilent IO Libraries Suite 16 1 is required The following steps are required to setup GPIB communication 1 Installing an GPIB Interface 79 2 Connecting the intrument s with the controller GPIB Cabling 80 3 Installation of the Agilent GPIB Communication Library 82 4 Configuration of the GPIB interface Configuring the Agilent 82350 GPIB Interface 86 Configuring the Agilent 82357 USB GPIB Interface 92 5 Configuring an Agilent Cary 8454 UV Visible Spectrophotometer 128 Operating System Instrument Compatibility The Agilent 82350A B GPIB interface PCI card and the Agilent 82357A B USB to GPIB interface are supported on Windows 7 The Agilent 82350 is a PCI GPIB interface card no additional settings on the interface card such as changing the I
37. PC 112 Installation of the TCP IP protocol 112 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 7 Contents Installing the LAN Interface in your UV Visible Spectrophotometer 115 Connecting the Spectrophotometer to the PC 116 Single Instrument Installation 116 Multiple Instrument Installation 116 Setting the IP Address of the Spectrophotometer 117 Using the Agilent BootP Service 118 What is the Agilent BootP Service 118 Addresses 119 Using the Agilent BootP Service Program 120 Configure the Agilent BootP Service Program 121 Configure the Agilent BootP Service 123 Configuring the G1369C LAN Card Using BootP 125 Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer 127 What is the Agilent ChemStation Configuration Editor 127 Configuring an Agilent Cary 8454 UV Visible Spectrophotometer 128 Troubleshooting LAN Communication 131 Power failure reported on the Agilent ChemStation 131 Frequent buffer overrun in Agilent Cary 8454 logbook 132 Safety Information 133 Safety Information 134 Warning Symbols 134 Precautions 136 Solvent Information 137 Flow Cells 137 Solvents 137 Other Precautions 139 Protective Earth 139 Disconnecting Device 139 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Contents Allowing Hot Instrument to Cool 140 Environmental Conditions and Power Requirements 141 Installation category 142 Pollution level 142 Electrical Power 14
38. SE AGREEMENT ATTENTION DOWNLOADING COPYING PUBLICLY DISTRIBUTING OR USING THIS SOFTWARE IS SUBJECT TO THE AGREEMENT SET FORTH BELOW TO DOWNLOAD STORE INSTALL OR RUN THE SOFTWARE YOU MUST FIRST AGREE TO AGILENT S SOFTWARE LICENSE AGREEMENT BELOW IF YOU HAVE READ UNDERSTAND AND do not accept the terms of the license agreement InstallShield Figure 26 Agilent 10 Libraries Suite license agreement Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 83 6 GPIB Communication Connections and Configuration Installation of the Agilent GPIB Communication Library Setup Type Choose the setup type that best suits your needs Please select a setup type Typical fie Recommended features for your configuration will be installed Custom Choose the program features you want to install Recommended for advanced users InstallShield Figure 27 Agilent IO Libraries Suite setup type selection 5 Click Next to continue and press the Install button on the summary screen ite 15 0 InstallShield Wizard Start Copying Files Review settings before copying files Setup has enough information to start copying the program files If you want to review or change any settings click Back If you are satisfied with the settings click Next to begin copying files Current Settings 10 Libraries Suite Directories Core Product Directory C Program Files Agilent I0 Libra
39. Spectroscopy System Installation Guide 21 2 Installation and Start Up Unpacking Your Spectrophotometer 22 Upon receipt of your spectrophotometer computer and printer inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the spectrophotometer computer or printer have been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier as well as Agilent Technologies Save the shipping material for the carrier s inspection Check the contents of the shipping container against the list below If any items are missing or obviously damaged call Agilent Technologies V Unpack the spectrophotometer You should have the following components Power cord to connect the spectrophotometer to line power LAN Interface if you connect the spectrophotometer through a LAN Waste tubing to connect the sample pan of your spectrophotometer to a waste container Checkout sample caffeine solution V Unpack the computer and its accessories You should have the following components Computer and power cable Keyboard Mouse Display unit connecting cable and power cable USB to GPIB Interface if you connect the spectrophotometer through GPIB Printer connecting cable and power cable Printer accessories for example ink cartridge in case of a DeskJet printer Agil
40. The GPIB address 25 is also used as the default in the installation of the UV Visible ChemStation software To change the GPIB address of your spectrophotometer 1 Turn off your spectrophotometer 2 On the spectrophotometer s configuration switch module make sure switches 1 and 2 are in the down position for GPIB address definition and set the switches 3 through 8 for the appropriate address see Table 5 E E E E E E E E 123 45 67 8 Figure 55 Configuration switch module Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 107 6 GPIB Communication Connections and Configuration 3 Remove any objects for example cuvettes flow cells from the light path and turn on the line power to the spectrophotometer This action stores the GPIB address in the non volatile memory The address will remain unchanged in the non volatile memory until a new address is set on the DIP switches and the instrument is power cycled again CAUTION Make sure that the GPIB address does not conflict with other instruments connected to the same GPIB controller and the GPIB address of the controller itself In our configuration the GPIB address 30 is used for the controller 108 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Address Settings Use Table 5 and Table 6 to find the switch settings for the GPIB address of your spectrophotometer GP
41. They automatically turn off the lamps when the sheet metal door is opened Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and StartUp 2 Setting up the Spectrophotometer Connecting Your Spectrophotometer via GPIB to the Computer 1 Identify the spectrophotometer s configuration switch module see Figure 3 For GPIB operation the default address is 25 that is all switches are in the down position except for the switches 4 5 and 8 which are in up the position resulting in a pattern 00011001 where 0 means down and 1 means up No two instruments or accessories may share the same GPIB address For alternate switch settings see Chapter 6 GPIB Communication Connections and Configuration 123 45 67 8 Figure 3 Configuration switch module 2 Connect the GPIB cable to the GPIB connector at the rear of the spectrophotometer Connecting your Spectrophotometer via LAN to the Computer see Chapter 7 LAN Communication Installation Connection and Configuration Connecting Power to Your Spectrophotometer See Figure 2 on page 25 1 Your line voltage must be between 100 240 V AC with a line frequency of 50 or 60 Hz There is no voltage selector on your spectrophotometer because the power supply has automatic wide ranging capability 2 Make sure the power switch of the spectrophotometer is in the off position that is the push
42. actions you must perform to install your UV Visible spectroscopy system If you are an experienced user of analytical equipment from Agilent Technologies this may be all you need If you need more information this chapter guides you to other relevant areas in this or another handbooks 2 Installation and Start Up Use this chapter if you need more detailed information about the installation process 3 Good Measurement Practices Use this chapter if you require information about general operating conditions to perform accurate and reliable measurements for example handling samples solvents and cells 4 Installing Accessories This chapter describes how to install the simple mechanical and optical accessories that are available to expand the capabilities of your UV Visible spectrophotometer 5 Installing the UV Visible Software This chapter describes what your computer requires and what you must do to install the software Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 3 GPIB Communication Connections and Configuration This chapter describes how to set the select code how to install an GPIB card in your computer and how to change the GPIB address of your spectrophotometer LAN Communication Installation Connection and Configuration This chapter describes how to set up LAN communication between your Agilent ChemStation and your UV Visible spectrophotometer Safety Information This chapter conta
43. anges by e g creating a log file entry of a new instrument and edit the new instrument using the Launch Manager Deselect Do you want to log bootp requests 10 Click OK to save the values or Cancel to discard them The program ends 11 To restart the BootP Service go to Start gt Settings gt Control Panel gt System and Security and select Administrative Tools gt Services The Service screen appears 12 Right click Agilent BootP Service 13 Select Start 14 Close the Services and Administrative Tools screen 15 This completes the configuration Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Configuring the G1369C LAN Card Using BootP In order for an internal G1369C LAN card to use BootP the default setting bootp YES must be set For more details please see the user manual of your G1369C LAN interface card To change the setting the card must be reset this is done each time the instrument with the G1369C LAN card inserted is power cycled The Agilent BootP Service uses a file called bootptab were all the configuration information is stored The BootP Service program on the Agilent ChemStation CD ROM allows easy editing of the bootptab file If a BootP Service is already installed the following information must be supplied to the system administrator to be able to correctly set up the bootptab file CAG Bootptab file global
44. ard will start see Figure 40 3 Select Option 1 and click Next and the installation will start see Figure 40 Found New Hardware Wizard Figure 40 Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Can Windows connect to Windows Update to search for software C Yes this time only Yes now and every time connect a device C No not this time Click Next to continue Found New Hardware Wizard first detection Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 93 6 GPIB Communication Connections and Configuration Configuring the Agilent 82357 USB GPIB Interface 4 To initialize the interface select Option 1 and click Next and the initialization will start see Figure 41 Found New Hardware Wizard Ss This wizard helps you install software for Agilent Technologies 823574 Initializing 9 If your hardware came with an installation CD QE or floppy disk insert it now What do you want the wizard to do i d 33 Install from a list or specific location Advanced Click Next to continue lt Back Next gt Cancel Figure 41 Found New Hardware Wizard first detection 5 The system will initialize the USB device This might take some seconds see Figure 42 Found New Hardware Wizard P
45. ardware Wizard Please choose your search and installation options Figure 33 Install hardware device drivers 3 Select 82350 PCI and click Next see Figure 34 The installation of the device driver will start Found New Hardware Wizard Select the device driver you want to install for this hardware Figure 34 Select a device driver 88 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Configuring the Agilent 82350 GPIB Interface 4 Click Finish see Figure 35 You are notified when the process is complete Completing the Found New Hardware Wizard The wizard has finished installing the software for Agilent Technologies 82350 PCI GPIB Click Finish to close the wizard lt Back i Cancel Figure 35 Finish installation of the GPIB card 5 Start the IO Config Utility by clicking in the IO Icon Purr iom and choose Agilent Connection Expert 6 Uncheck Show this window at startup and press Close Welcome Screen The Agilent Connection Expert Main Window is displayed see Figure 36 ouan snin Agilent VSA The primary VSA ibrary Figure 36 10 Libraries Configuration Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 89 GPIB Communication Connections and Configuration Configuring the Agilent 82350 GPIB Interface 7 In the Agilent Connection Expert main window select GPIBO see Fi
46. ble spectrophotometer is connected to the PC s LAN interface In this configuration only the black 10 ft long crossover twisted pair cable part number 5183 4649 can be used This is supplied with the Agilent UV Visible ChemStation software Do not use the standard non crossover Ethertwist 10BaseT cable supplied with the LAN interface card G1846A for direct single instrument connection Multiple Instrument Installation If you want to connect more than one spectrophotometer to the Agilent ChemStation PC or if the PC needs to connect to a network using the same network connection a switch for example the G2402A 8 port switch is required The G2402A switch comes with one standard EtherTwist 10BaseT cable that is used for the connection to the PC Use the standard EtherTwist 10BaseT cable part number G1530 61485 provided with the LAN interface cards to connect the switch to the spectrophotometer Do not use the black 10 ft long crossover twisted pair cable part number 5183 4649 in an installation with multiple instruments 116 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Use standard twisted pair LAN cables for the connections Personal computer Network Switch Instruments Figure 58 LAN connection using a switch Setting the IP Address of the Spectrophotometer The IP address of the LAN interface card inserted
47. bon disulfide 43 carbon tetrachloride 43 CE compliance 143 chloroform 43 cleaning cells 41 colloidal dispersions 44 common solvents 43 computer video socket 29 configuration editor 73 128 configuration switch 8 bit configuration switch 26 configuration switch module 27 connecting power to your spectrophotometer 27 connecting tubing 51 consumption line power 19 contents of shipping container 22 control switch module 107 cooling air 20 cut off filter 45 cut off wavelength 45 cyclohexane 43 cyclopentane 43 D default address 27 degassed 44 device configuration 73 device configuration dialog 129 dimethyl formamide 43 dimethyl sulfoxide 43 DIP switches 108 dirty filters 60 display 29 dust 44 E electronic self tests 31 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide entrapment of bubbles 40 Environmental Conditions and Power Requirements 141 error condition 24 25 ethyl acetate 43 ethyl ether 43 excessive noise 33 F factory firmware settings 109 fan 31 flow cell 40 flow cells 48 137 frequency line power 19 front panel indicator 24 front panel pushbuttons 25 front view 23 fuses 19 G general purpose input output connector 26 glass cells 37 glycerol 43 GPIB cable 80 GPIB address 27 GPIB address definition 107 GPIB cable 27 GPIB connector 26 27 GPIB switch module 26 GPIO connector 26 ground co
48. button stands out and the LED inside the switch is off see Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 27 2 Installation and Start Up Figure 1 3 Plug the female end of the power cord into the power input socket at the rear of the spectrophotometer Plug the male end of the power cord into your electrical outlet Installing the Waste Tubing There is a waste outlet for the sample area It is used to drain any liquids which may come from the sample area in case of a leak or a spill to a waste container To connect your waste tubing to the spectrophotometer 1 Lift the front part of the spectrophotometer up so that you can locate the waste outlet underneath the sample pan Figure 4 Connecting the waste tubing 2 Connect the waste tubing which comes with your spectrophotometer to the waste outlet underneath the sample pan 3 Put the other end of the waste tubing into the waste container 4 Make sure that the waste tubing has a constant down slope from the spectrophotometer to the waste container 28 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and Start Up 2 Installing Your Computer Detailed instructions for installing your computer are specific to the type and configuration of the computer and are provided with it This section gives a checklist of the main installation procedures refer to the documentation that was delivered with your computer for d
49. ce measurement of solvent without the analyte This is used in all subsequent sample measurements until a new blank measurement is taken A baseline spectrum is displayed on the computer e sample the instrument takes a sample measurement or starts a series of measurements This depends on the parameters set in your software e standard the instrument takes a measurement of a standard Additional information such as concentration and so on have to be entered in the operating software e stop the instrument and or software aborts any ongoing activity and returns to a to measure state All connections are made at the rear of the spectrophotometer see Figure 2 GPIB connector and switches MIO slot for LAN interface Remote connector on D Multicell transport connector GP 10 connector Line connector RS 232 connector Figure 2 Rear view of the Agilent Cary 8454 UV Visible spectrophotometer Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 25 2 26 Installation and Start Up The multi cell connector allows you to connect the cable which comes from the multicell transport The GPIO general purpose input output connector allow you to connect your sipper and autosampler or other accessories The remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare etc
50. ce your spectrophotometer has passed the self tests it is ready to run samples For best results you may want to let the spectrophotometer warm up for at least 1 hour This time allows the optical system to stabilize and should result in better measurements If the instrument was not stored at room temperature before it was turned on allow more time for the instrument to stabilize In general the life of your spectrophotometer will be increased by leaving it on all the time but switching off the lamps when the instrument is not in use lamp intensity of the deuterium lamp and lifetime of lamps decrease with use Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and Start Up 2 Starting the Agilent ChemStation Software If the Agilent UV Visible ChemStations icon is not visible on the Windows Desktop open the Start menu and select Agilent UV Visible ChemStations from the list of programs A maximum of two sessions of an instrument can be started in parallel the online session must be always the first launched session Two instrument icons are displayed for the Agilent UV Visible ChemStations e Instrument Online starts the software in online mode online means Agilent ChemStation is connecting to the configured spectrophotometer Instrument Offline starts the software in offline mode offline means Agilent ChemStation is not connecting to the configured spectrophotometer To start the software clic
51. cell holder the adjustable cell holder or the thermostattable cell holder An optical filter wheel with the same filters but with a different mounting can be used with the multicell transport see the multicell transport User s Guide Notches indicate filter number Figure 20 Optical filter wheel assembly The filter wheel rotates to four different positions Position zero is empty and permits 100 transmittance at all light wavelengths Positions one two and three have filters which absorb varying amounts of the UV spectrum Figure 21 shows the transmission characteristics of each filter Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 59 4 60 Installing Accessories 100 80 60 40 20 190 200 300 400 500 600 700 800 820 Figure 21 Transmission characteristics of filter wheel filters Table3 Filter wheel positions Position Filter 0 None 1 UV roll off 2 265 nm UV cut off at 50 T 3 295 nm UV cut off at 50 T The choice of when to use a filter and which filter to use should be made only after considering all the factors that affect your measurements If you decide that a filter is needed you should choose a filter that transmits the wavelengths needed for analytical information and that blocks the wavelengths that contribute to sample degradation Often your final choice will be a compromise since sometimes even the light at the wavelengths of analytical
52. d every time connect a device C No not this time Click Next to continue Figure 44 Found New Hardware Wizard second detection Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 95 96 GPIB Communication Connections and Configuration Configuring the Agilent 82357 USB GPIB Interface 9 Select Option 1 and click Next to start the installation see Figure 45 Found New Hardware Wizard This wizard helps you install software for Agilent Technologies 823574 Initializing If your hardware came with an installation CD or floppy disk insert it now What do you want the wizard to do Install from a list or specific location Advanced Click Next to continue lt Back Figure 45 Found New Hardware Wizard second detection 10 The system initializes the 82357A USB GPIB Interface device This might take some seconds see Figure 46 Wait until the configuration screen appears see step 14 Found New Hardware Wizard Please wait while the wizard installs the software SS pig Agilent Technologies 823574 Initializing re Sw agt357 inf To CAWINDOWSSINF Figure 46 Installing the 82357A USB GPIB interface second detection Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Panlkin aa oh A Configuring the A Hardware Installation The software you are installing for this hardware
53. e Spectroscopy System Installation Guide Safety Information A Regulatory Information CE Compliance Your Agilent instrument has been designed to comply with the requirements of the Electromagnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Agilent has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by e the CE Marking appearing on the rear of the product and the documentation package that accompanies the product containing a copy of the Declaration of Conformity The Declaration of Conformity is the legal declaration by Agilent that the product complies with the directives listed above and shows the EN standards to which the product was tested to demonstrate compliance Electromagnetic Compatibility EN55011 CISPR11 Group 1 ISM equipment group 1 contains all Industrial Scientific and Medical ISM equipment in which there is intentionally generated and or used conductively coupled radio frequency energy which is necessary for the internal functioning of the equipment itself Class A equipment is equipment suitable for use in all establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purpos
54. e filter wheel assembly The mounting ridges on the filter wheel assembly should fit into the holes in the clamp The base of the filter wheel assembly should be against the base of the cell holder 6 Tighten the screws that hold the clamp Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 61 4 62 Installing Accessories Removing the Filter Wheel Assembly Loosen but do not remove the two screws that hold the clamp 2 Separate the filter wheel assembly from the cell holder The filter wheel clamp may be left in place or removed In either case retighten the two screws 4 Store the filter wheel in a clean plastic bag Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System P Installation Guide ee 5 Installing the UV Visible Software 6 e z e a Agilent ChemStation Computer Details 64 Installing the Agilent ChemStation Using the DVD 67 This chapter describes the minimum hardware and software requirements and describes the installation process for the UV Visible software It is assumed that a supported Microsoft Windows operating system is already installed Agg Agilent Technologies 63 5 Installing the UV Visible Software Agilent ChemStation Computer Details This section describes the PC hardware and operating system requirements that must be met for successful installation and operation of Agilent UV Visible Ch
55. e of the products in violation of applicable laws rules or regulations Warning Symbols To avoid personal injury death or damage to the instrument the operator must refer to an explanation in the manual This symbol is placed on the product where it is necessary for you to refer to the manual in order to understand a hazard 134 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Safety Information A The following symbols appear on the instrument for your information The hazard they describe is also shown Single phase alternating current When attached to the rear of the instrument indicates that the product complies with the requirements of one or more EU directives Indicates a protective ground terminal Eye damage may result from directly viewing light produced by deuterium lamps used in detectors and spectrophotometers Always turn off the deuterium lamp before opening the lamp door on the instrument Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 135 A Safety Information Precautions 136 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective ear
56. e that your spectrophotometer computer and the UV Visible operating software is in proper operating condition There is an optional automated self test which can be used for a quick check of the spectrophotometer This self test can only be executed after the instrument has warmed up for at least one hour 18 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and StartUp 2 Site Requirements for Your Spectrophotometer Power considerations Your spectrophotometer can operate on a line voltage of 100 240 V AC witha line frequency of 50 or 60 Hz Maximum power consumption is 220 VA The instrument power supply accepts any line voltage supplied in the above mentioned range Consequently there is no voltage selector in the rear of the spectrophotometer There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING To disconnect the instrument from line power disconnect the power cord from the supply The power supply still uses some power even if the power switch on the front panel is turned off The computer and InkJet or LaserJet printers accept a nominal line voltage of 110 or 220 V AC with a line frequency of 50 or 60 Hz For more information see the documentation that comes with your computer and or printer WARNING Connecting your instrument to a line voltage that is higher than specified causes a shock hazard and damage may occur to
57. each time it is started If a DHCP server is running in the same subnet make sure that fixed IP addresses are reserved for your instruments Ask you IT consultant to make sure that no IP address conflict may occur Frequent buffer overrun in Agilent Cary 8454 logbook Please contact your system administrator to check if the network is not capable of the network traffic induced due to the data acquisition of the instrument s This can also be caused by an incorrectly configured buffer handling of the LAN interface card correct settings T145 64 T146 01 T147 01 132 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Operator s Manual A Safety Information Safety Information 134 Warning Symbols 134 Precautions 136 Solvent Information 137 Other Precautions 139 Environmental Conditions and Power Requirements 141 Regulatory Information 143 Agilent Technologies on the Internet 145 ee Agilent Technologies 133 A Safety Information Safety Information Agilent products must only be used in the manner described in the Agilent product user guides Any other use may result in damage to the product or personal injury Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or us
58. ed if you do not connect to any other part of a network 6 Turn off the Microsoft Windows Firewall and reboot the computer 114 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Installing the LAN Interface in your UV Visible Spectrophotometer The JetDirect card G1846A and the TalkToLab card G1369C are the LAN interfaces used to physically connect the spectrophotometer to the PC viaa LAN cable To install the LAN interface card 1 Switch off the spectrophotometer 2 Remove the metal cover at the upper right of the rear panel 3 Ifthe system is on an isolated LAN the following defaults can be used 192 168 254 10 for the PC IP address 255 255 255 0 for the subnet mask and 192 168 254 11 for the spectrophotometer note to use these defaults DIP switches 5 and 6 on the LAN card must be ON 4 Plug the LAN interface card into the slot and screw down the two mounting screws Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 115 7 LAN Communication Installation Connection and Configuration Connecting the Spectrophotometer to the PC This section describes how to setup the connection between your instrument s and the PC If you are going to connect to an existing LAN please contact your system administrator for further assistance Single Instrument Installation In a single instrument installation only one UV Visi
59. elete Task Guide DEK System tasks iP Refresh al RRB Add an instrument OF com ASRL1 SF come ASRL2 BR sodan interface SA LAN TCPIPO hee information 4 usB GPIB GP1B0 GPIBO 25 INSTR S usso How do 1 add an instrument Q How dot get drivers Where can I find programming samples Agilent VISA The primary VISA library Figure 48 Agilent Connection Expert main window 82357 USB GPIB interface Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Configuring the Agilent 82357 USB GPIB Interface 14 In the Agilent Connection Expert main window select USB GPIB GPIB0 Go Agilent Connection Expert File Edit view I O Configuration Tools Help X ReteshAll oe DProperties I Interactive 10 HR Add instrument PJ Add Interface XK Delete Task Guide Instrument 1 0 on this PC Agilent 82357A Interface Converter USB GPIB GPIBO Tasks for This Interface k Refresh All 2 Refresh this GPIB interface a kwapa R Change properties S comi ASRLI Change Properties G COM2 ASRL2 DP change the label amp Lan crcptpo s VISA interface ID GPBO Less lt lt Pa Serial number MY45346096 XK perte GPB address z General Tasks SICL interface ID gpibo Logical unit 7 ig Refresh all a Board number 0 GPIB0 Agilent 488 Properties IRR Add an instrument Pae decor Yes More I
60. emStation Agilent UV Visible ChemStation is supported on personal computers with a 3 GHz 32 bit x86 processor or better All PC hardware and peripherals must be listed in the Microsoft Windows Marketplace Tested Products List Hardware section The hardware testing status is available from the Microsoft home page on the world wide web http winqual microsoft com HCL Default aspx If your PC hardware is not tested we cannot guarantee that the system will work with the supported operating system The software will run with the minimum amount of RAM installed but system performance will depend on what other processes are running Minimum PC Requirements for Agilent ChemStation Table4 Minimum PC Requirements PC configuration Windows 7 Professional 32 bit SP 1 Graphics resolution The recommended graphics resolutions for video graphics adapter are 1024 x 768 with large fonts or 1280 x 1024 with large fonts Processor 3 GHz Screen resolution Super VGA 1280 x 1024 64 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing the UV Visible Software 5 Table4 Minimum PC Requirements continued Mass storage 40 GB Hard Disk and CD ROM Memory Minimum of 2 GB of RAM for UV Visible ChemStation software and a single instrument The minimum recommended amount of memory for a single bath dissolution system G1118AA and kinetic measurements G1117AA is 3 GB of RAM Add 256 MB of RAM for each addi
61. ended Standard Figure 61 Services window 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools windows Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 123 7 124 LAN Communication Installation Connection and Configuration To edit the BootP Settings go to Start gt All Programs gt Agilent BootP Service gt Edit BootP Settings The BootP Settings screen appears When this screen opens the first time the default settings from installation are displayed To edit the TabFile select Maintain bootp tabfile The default tab file was created at installation and is located at Program Files Common Files Agilent Shared BootP bin TabFile It contains configuration information entered on this screen If necessary you can change the BootPtab File Location by using the browse button on the right a valid BootP tabfile must exist create your own template for the tabfile by selecting Create template BootP tabfile and click Create Template Select Do you want to log bootp requests The default log file was created at installation and is located at Program Files Common Files Agilent Shared BootP bin LogFile It contains an entry for every time a device requests configuration information from BootP If necessary you can change the BootPlog File Location by using the browse button on the right a valid BootP tabfile must exist Perform your necessary ch
62. ent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and Start Up 2 Installing your UV Visible Spectrophotometer Your spectrophotometer is very easy to install This section provides an overview of the instrument and a checklist of the main installation procedure If you have accessories for the spectrophotometer which are not covered in this section see Chapter 4 Installing Accessories or refer to the documentation that was delivered with your accessories for detailed information Instrument overview The front view of the spectrophotometer is shown in Figure 1 which shows the sample compartment open Unlike conventional instruments the UV Visible spectrophotometer does not suffer from ambient stray light The open sample area makes it easier to access it generally and to connect tubing to a flow cell or thermostattable cell holder The spectrophotometer is shipped with the standard single cell cell holder already in place Standard and accessory cell holders can be removed and replaced in seconds with few or no tools Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 23 2 Installation and Start Up green light 24 Line power switch with Pushbuttons Figure 1 Front view of the Agilent Cary 8454 UV Visible spectrophotometer The line power switch is located at the lower left part of the instrument Pressing it in turns on the instrument It stays pressed in and shows a green
63. erting a Cell 48 This chapter describes making measurements selecting material optical specification and type of cell handling and maintaining cells checklist for good results solvents selection sample preparation use of filters stirring and temperature control of sample how to insert cuvettes into the cell holder phe Agilent Technologies 35 3 Good Measurement Practices General Considerations There are many factors that can affect the results of your measurements This section provides brief discussions of some of the more important ones Spectrophotometer Design The sample compartment of the spectrophotometer is open Unlike conventional instruments the Agilent Cary 8454 UV Visible spectrophotometer does not suffer from ambient false light The open sample area is easier to access and it is easier to connect tubing to a flow cell or thermostattable cell holder Making Measurements Blank Reference and Sample Measurement Your spectrophotometer is a single beam instrument so you must measure a blank before you measure a sample For high accuracy measurements the blank and the sample measurement should closely follow each other In general a blank measurement should be repeated as often as is practical Even in a thermally stable environment a blank measurement should be taken every half hour to ensure accurate results Chemically the only difference between the blank and the sample should be t
64. es This device complies with the requirements of CISPR11 Group 1 Class A as radiation professional equipment Therefore there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 143 A 144 Safety Information Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures 1 Relocate the radio or antenna 2 Move the device away from the radio or television 3 Plug the device into a different electrical outlet so that the device and the radio or television are on different electrical circuits 4 Make sure that all peripheral devices are also certified 5 Make sure that appropriate cables are used to connect the device to peripheral equipment 6 Consult your equipment dealer Agilent Technologies or an experienced technician for assistance Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment ICES NMB 001 This ISM device co
65. etailed information Setting up the computer V If you have any additional accessories install them before you set up your computer Additional memory refer to your computer documentation Additional mass storage devices hard disk drive tape drive DVD refer to your computer documentation Accessory boards excluding USB to GPIB interface refer to your computer documentation USB to GPIB interface see Installing an GPIB Interface on page 79 V Connect the keyboard and mouse to the sockets at the back of the computer V Connect the display unit to the video socket at the back of the computer V Connect the printer cable to the socket on the printer and to the correct printer interface socket on the back of the computer V Connect the GPIB connector of the USB to GPIB interface to the spectrophotometer s GPIB connector and the USB connector to the computer or connect your LAN cable from the spectrophotometer to the LAN connector on the computer If you have more GPIB instruments connected to your computer see Chapter 6 Installing an GPIB Interface for correct GPIB cabling because you must observe some basic rules For correct setup see Chapter 7 LAN Communication Installation Connection and Configuration VY Connect the power sockets to the computer display and printer and switch them on Follow the instructions for setting up the computer in your computer documentation Agilent Cary 8454 UV Visib
66. g module 45 stop pushbutton 25 stoppered sample cell 44 stray light 23 sulfuric acid 43 supply line power 19 switches power 27 T TalkToLab 115 temperature control 44 temperature fluctuations 21 temperatures 21 thermal equilibrium 51 thermostattable cell holder 45 thermostatted water 51 thermostatted water bath 50 toluene 43 trimethylpentane 2 2 4 trimethylpentane 43 turn on your spectrophotometer 30 turn on self test 33 U useful wavelength range of solvents 43 UV grade water 44 V video socket on computer 29 viscous solutions 45 volatile solvents 44 voltage line power 19 wW Warning Symbols 134 warning symbols 134 waste container 28 water 43 wedge shaped cells 38 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Index 149 Index 150 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide www agilent com In This Book This handbook describes the steps needed to install your new Agilent Cary 8454 UV Visible spectroscopy system Most of the handbook deals with installing a complete system that is spectrophotometer computer and printer However in the second half of this handbook you will find additional information about installing mechanical and optical accessories accessory boards GPIB connections and configuration and software installation Agilent Technologies 2002 2003 2008 2011 2013 2014 0
67. gned by the BootP request gw lt gateway address ask your system administrator gt This is the gateway address assigned by the BootP request T145 64 T146 01 T147 01 These parameters configure the buffer handling of the G1369C LAN card These are required for the instrument on the LAN to work properly and must not be changed Different entries for the subnet mask and default gateway might be required depending on your network setup These values need to be set by your network administrator 126 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer What is the Agilent ChemStation Configuration Editor The Agilent ChemStation Configuration Editor is a program that allows the easy configuration of your Agilent UV Visible ChemStation software This includes e Detecting the GPIB interface in your PC e Selecting communication using LAN or GPIB e Configuring the analytical hardware connected to the PC e Configuring the paths used for data storage e Configuring color display of the Agilent ChemStation Since the Configuration Editor modifies the internal communication module database the Agilent UV Visible ChemStation must be closed beforehand If LAN connection is used Microsoft TCP IP protocol must be installed and configured the LAN interface Ca
68. gure 37 2 WBE leteractive lO M Add incoumert PY Addinterlace Y Delete ere CSC a Refresh ad 2B Kure SY coon cases SF come aseaz Tasks for This Interface RO Refresh this GPIB terface S uso a1101039 2 ves Agtent VISA The primary VI A brary Figure 37 Agilent 82350 default settings 8 Click Change Properties In the Agilent 82350 PCI GPIB Interface GPIBO dialog window select or enter the following properties VISA interface ID to GPIBO GPIB address to 30 check System controller recommended SICL Interface ID to hp82341 note that this is case sensitive Logical unit to 7 or 9 and uncheck Auto discover instruments connected to this interface see Figure 38 Agitent 82350 P PIG Interlace GPO VBA interface 1 creo CPB address w system coneroter irecommensed SICK interface ID noBz3st Logical unit 7 Auto discover instruments connected to this interface Ajar 488 Properties Ca Cow Cie Figure 38 Agilent 82350 interface configuration The appearance of the dialog slightly differs depending on the type of GPIB board installed NOTE pp g Slightly p 90 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 DIR Intarfara intertace Configuring the Agilent 82350 GPIB 9 Select OK The configured GPIB board should now appear as GPIBO in the list of Instrument I O on this P
69. he Agilent 89055A cell stirring module shown in Figure 14 mounts inside the base of the thermostattable cell holder It enables magnetic induction stirring inside a standard 1 cm sample cell The cell stirring module contains a plastic encased magnetic impeller that can be driven by water or pressurized air When you put a magnetic stirring bar 9301 1161 into the filled sample cell the stirring bar rotates in tandem with the impeller magnet thus stirring the sample Figure 14 Cell stirring module Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing Accessories 4 Installing the Cell Stirring Module Remove the thermostattable cell holder from the sample compartment 2 Remove the three screws that hold the thermostattable cell holder to its base Remove the cell holder and turn it upside down 3 Insert the cell stirring module into the cavity in the base of the thermostattable cell holder see Figure 15 Figure 15 Installing the cell stirring module 4 Tighten the four screws flathead M3x6 mm that hold the cell stirring module in place 5 Attach the thermostattable cell holder to its base 6 Mount the thermostattable cell holder back in the sample compartment Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 53 4 54 Installing Accessories Tubing Connections and Operation The cell stirring module can be driven by either water or air Water is preferable because i
70. he instrument operates within performance specifications Your spectrophotometer can be stored at altitudes up to 4 600 m 14 950 ft and operated at altitudes up to 2 000 m 6 500 ft The Agilent Cary 8454 UV Vis instrument is suitable for indoor use only and is classified suitable under Equipment class I category Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 141 A 142 Safety Information Installation category The installation category is II based on IKC61010 1 The installation category implies the regulation for impulse withstand voltage It is also called the Over voltage category II applies to electrical equipment with a nominal supply voltage up to 300 V Pollution level The pollution level is 2 based on IEC61010 1 Pollution level describes the degree to which a solid liquid or gas that deteriorates dielectric strength is adhering 2 applies to a normal indoor atmosphere where only non conductive pollution occurs Electrical Power Your spectrophotometer can operate on a line voltage of 100 240 VAC witha line frequency of 50 or 60 Hz Maximum power consumption is 220 VA The instrument power supply accepts any line voltage supplied in the above mentioned range Consequently there is no voltage selector in the rear of the spectrophotometer There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Agilent Cary 8454 UV Visibl
71. he optional adjustable cell holder The adjustable cell holder helps you ensure the cells are properly centered in the light path 48 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 4 Installing Accessories Thermostattable Cell Holder 50 Cell Stirring Module 52 Long Path Cell Holder 56 Optical Filter Wheel 59 Many simple mechanical optical accessories are available to expand the capabilities of your UV Visible spectrophotometer This section briefly describes these accessories and outlines how to install them The following more complex accessories with electronic components are available to enhance the functionality and capabilities of your spectrophotometer Information about these are included with the accessories in separate handbooks e Sipper system e Autosampler e Multicell transport e Peltier temperature control accessory ee Agilent Technologies 49 4 Installing Accessories Thermostattable Cell Holder The Agilent 89054A thermostattable cell holder see Figure 13 is a single cell cell holder with a manifold around the cell Water from a thermostatted water bath can be circulated through the manifold to hold the sample cell at a uniform temperature Up to 1 cm open topped cells and flow cells can be used in a thermostattable cell holder NO RN EERE Tubing restraint tab Figure 13 Thermostattable cel
72. he presence of the analyte s For measurements with liquid samples the blank should be a sample cell filled with the solvent you plan to use 36 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Good Measurement Practices 3 Sample Cell or Cuvette Material Quartz sample cells cuvettes or sample cells with quartz face plates are required if you want to use the full 190 to 1100 nm wavelength range of your spectrophotometer If you plan on working only in the visible and or short wave near infrared range of 350 to 1100 nm you can use good quality glass cells Disposable plastic sample cells for measurements in the range 400 1100 nm are also available The quality of these cells varies and they are generally not recommended Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 37 3 Good Measurement Practices Optical Specifications of Cells The accuracy of the readings of a diode array instrument is very sensitive to spatial shifts of the analysis light beam Cells having non parallel opposite faces or so called wedge shaped cells lead to a spatial shift of the light beam see Figure 6 Therefore the opposite cell walls illuminated by the analysis light beam have to be parallel The degree of parallelism is measured in terms of the angle between the two opposite cell walls We recommend that you use 10 mm path length cells with an angle which is below 0 1 degrees of an arc
73. her express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or asa commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclo sure of Software is subject to Agilent Tech nologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rig
74. hts as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide In This Guide This handbook describes what you have to do to install your UV Visible spectroscopy system Most of the handbook deals with installing a complete system that is the spectrophotometer computer and printer However in the second half of this handbook you will find additional information about installing accessories accessory boards GPIB connections and configuration and software installation 1 Fast Installation Use this chapter as an overview of the different
75. ins relevant safety information Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Contents 1 Fast installation 11 Installing Preconfigured Agilent Cary 8454 UV Visible Systems 12 Installing Non Preconfigured Agilent Cary 8454 UV Visible Spectrophotometer Systems 13 Installing Software Only 15 2 Installation and Start Up 17 Site Requirements for Your Spectrophotometer 19 Power considerations 19 Power cords 20 Space 20 Environment 21 Unpacking Your Spectrophotometer 22 Installing your UV Visible Spectrophotometer 23 Instrument overview 23 Setting up the Spectrophotometer 27 Installing Your Computer 29 Setting up the computer 29 Starting the Spectrophotometer 30 TurningOn 30 Instrument Warm up 32 Starting the Agilent ChemStation Software 33 Operational Checkout 33 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Good Measurement Practices 35 General Considerations 36 Spectrophotometer Design 36 Making Measurements 36 Sample Cell or Cuvette Material 37 Optical Specifications of Cells 38 Apertured Cells or Cuvettes 39 Flow Cells 40 Handling and Maintaining Cells or Cuvettes 41 Solvents 43 Sample Preparation 44 Photosensitive Samples 45 Stirring and Temperature Control 45 Checklist for Best Results 46 Inserting a Cell 48 Installing Accessories 49 Thermostattable Cell Holder 50 Installing a Thermostattable Cell Holder 51 Cell Stirring Module 52 Installing the Cel
76. interest can affect your sample If you do not need a filter for a group of measurements you can move the filter wheel to position zero This permits full light transmission When you will not be using the filter wheel for an extended period of time you should remove the filter wheel assembly from the cell holder and store it in a clean plastic bag The optical filters of the filter wheel must be kept clean Dirty filters interfere with light transmission and lower the spectrophotometer performance These filters may be cleaned with isopropanol and photographic lens tissues Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing Accessories 4 Installing the Filter Wheel Assembly Installing the filter wheel assembly is fairly simple The procedure is the same for all three cell holders You will need a Phillips screwdriver to install the filter wheel assembly However if you install the filter wheel on the long path cell holder instead of a screwdriver a 2 5 mm hex wrench is required 1 Remove the cell holder from the sample compartment 2 Remove the two screws shown in Figure 22 3 Position the filter wheel assembly clamp as shown in Figure 22 Replace the two screws but do not tighten them J l Clamp Figure 22 Installing the optical filter wheel assembly 4 Tilt the outer edge of the clamp up slightly 5 Bring the filter wheel assembly against the cell holder so that the clamp fits in the slot of th
77. ion and Configuration Installing and Configuring TCP IP onthe PC 112 Installing the LAN Interface in your UV Visible Spectrophotometer 115 Connecting the Spectrophotometer to the PC 116 Using the Agilent BootP Service 118 Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer 127 Troubleshooting LAN Communication 131 The first five sections of this chapter describe all necessary installations connections and configurations of the LAN communication The last section of this chapter helps you if the LAN communication does not work properly ot Agilent Technologies 111 7 LAN Communication Installation Connection and Configuration Installing and Configuring TCP IP on the PC 112 Installation of the TCP IP protocol 1 Select Start gt Control Panel gt Network and Sharing Center In the Tasks list click on Manage network connections In the LAN or High Speed Internet list right click on your LAN adapter icon and select Properties 4 Local Area Connection Properties m m Networking Connect using Broadcom NetXtreme Gigabit Ethernet This connection uses the following items E Client for Microsoft Networks E QoS Packet Scheduler 8 File and Printer Sharing for Microsoft Networks Internet Protocol Version 6 TCP IP v6 Sg Internet Protocol Version 4 TCP IP v4 Link Layer Topology Discovery Mapper 1 0 Driver Link Layer Topology Discovery Responder
78. k the Instrument Online or Instrument Offline icon in the Agilent ChemStations section Operational Checkout Operational checkout is used to determine if the instrument is in proper operating condition in combination with your UV Visible operating software 1 Be sure to verify the successful completion of the turn on self test of the spectrophotometer that is the indicator light on the front panel of the spectrophotometer is lit and is green 2 In your UV Visible operating software take a blank measurement and observe the baseline noise With an empty sample area this should be in the low mAU range Excessive noise or spikes may indicate a problem 3 For the spectrophotometer an additional optional qualification can be automatically performed using your software After warm up of the spectrophotometer for one hour the instrument is ready for the automated self test which is located in the diagnostic portion of the software The successful completion of these tests complete the operational qualification of your spectrophotometer For reference purposes you may want to print a copy of the results Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 33 2 installation and Start Up Operational Checkout 34 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide ee 3 Good Measurement Practices General Considerations 36 Ins
79. l Stirring Module 53 Tubing Connections and Operation 54 Long Path Cell Holder 56 Installing the Long Path Cell Holder 57 Installing a Sample Cell 57 Optical Filter Wheel 59 Installing the Filter Wheel Assembly 61 Removing the Filter Wheel Assembly 62 Installing the UV Visible Software 63 Agilent ChemStation Computer Details 64 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Contents Minimum PC Requirements for Agilent ChemStation 64 Installing the Agilent ChemStation Using the DVD 67 Overview 67 Uninstalling Pre existing Agilent UV Visible ChemStation Software 67 Installation Procedure 68 Monitor Configuration 72 Configuring Your System 73 6 GPIB Communication Connections and Configuration 77 GPIB Communication 78 Operating System Instrument Compatibility 78 Installing an GPIB Interface 79 GPIB Cabling 80 Installation of the Agilent GPIB Communication Library 82 Installing New l0 Libraries 82 Configuring the Agilent 82350 GPIB Interface 86 Configuring the Agilent 82357 USB GPIB Interface 92 Upgrading Existing 10 Libraries 101 Upgrading 10 Libraries 101 Working with your Agilent 10 Libraries 102 Troubleshooting 103 Missing files during installation 103 Problems with Plug and Play option GPIB cards 105 Changing the GPIB Address of the Spectrophotometer 107 GPIB Address Settings 109 7 LAN Communication Installation Connection and Configuration 111 Installing and Configuring TCP IP onthe
80. l holder 50 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing Accessories 4 Installing a Thermostattable Cell Holder 1 Loosen the hold down screws and remove the current sample holder from the sample compartment 2 Orient the thermostattable cell holder the same way the standard cell holder was oriented and lower it onto the sample compartment mounting guides 3 Tighten the hold down screws 4 Connect tubing silicon 3 16 inch i d to the inlet and outlet of the manifold It does not matter which side is used as the inlet and which side is used as the outlet If necessary route the tubing under the tubing restraint tab to keep it from interfering with the light beam Make sure the tubing is securely attached it expands when heated and if it is not secure a leak will result 5 Insert the sample cell and lower the lever down to lock the cell in place 6 Begin pumping thermostatted water through the manifold Wait for the sample cell and sample to reach thermal equilibrium before you make any measurements The time required to reach thermal equilibrium will vary depending on the water temperature and the sample but generally should take about five minutes When the thermostattable cell holder is not in use it is best to disconnect the tubing and drain the manifold Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 51 4 Installing Accessories Cell Stirring Module 52 T
81. le Spectroscopy System Installation Guide 29 2 Installation and Start Up Starting the Spectrophotometer 30 Before you turn on your spectrophotometer make sure the light path through the sample compartment is clear All filters should be removed from the light path Anything blocking or absorbing part of the light beam could cause an error indication when you turn on the spectrophotometer The power switch is located at the left front of the spectrophotometer When starting UV Visible application software wait until the completion of the self test procedures for the spectrophotometer Otherwise the application software may not recognize the spectrophotometer Turning On 1 turn on your spectrophotometer with the pushbutton at the lower left part of the instrument The pushbutton will stay pressed in and light green if power is present Indicator Line power switch with green light Figure 5 Turning on the Agilent Cary 8454 UV Visible spectrophotometer Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and Start Up 2 2 The fan will start turning 3 The indicator at the upper left corner of the front panel will light in three different colors The yellow color which means busy will stay on during the start up and self test routines until at least one of the lamps are ignited and the spectrophotometer is ready to operate 4 The turn on procedure including electronic self tests lasts abou
82. lease wait while the wizard installs the software S ae y Agilent Technologies 82357A Initializing n ars ld Lod agt357 inf To C WINDOWS INF lt Back NERY ancal Figure 42 Initializing the 82357A USB GPIB interface first detection 94 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Configuring the Agilent 82357 USB GPIB Interface 6 Click Finish to close the wizard after the initialization Hardware driver see Figure 43 The initialization of the USB device is finished the installation still needs to be done Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for Agilent Technologies 82357 Click Finish to close the wizard lt Beck y Canca Figure 43 Finish Device Driver Installation 7 The hardware wizard now detects the USB GPIB device for a second time and the Found New Hardware Wizard starts again see Figure 44 8 Select Option 1 and click Next see Figure 44 Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission fis Can Windows connect to Windows Update to search for software Yes this time only Yes now an
83. ll is used V Time between blank and sample measurement should be short Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Good Measurement Practices 3 200 400 600 800 1000 0 0015 0 0010 0 0005 0 0000 Absorbance 0 0005 0 0010 200 400 600 800 1000 Wavelength nm Figure 11 Example of a blank on water showing a good baseline 0 01 0 00 0 01 0 02 Absorbance 0 03 0 04 0 05 200 400 600 800 1000 Wavelength nm Figure 12 Example of a blank on water with bubbles causing a poor baseline If your blank or spectra show artifacts similar to the one in Figure 12 see Solvents on page 43 to optimize the measurement procedure Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 47 3 Good Measurement Practices Inserting a Cell Your spectrophotometer is shipped with the standard single cell cell holder you must first install in the sample compartment This cell holder accommodates standard cells or flow cells To insert a sample cell cuvette in the cell holder 1 Move the locking lever to its up position 2 Insert the sample cell making sure you orient it correctly The frosted non clear sides of the sample cell should not be in the path of the light beam 3 Lock the sample cell in place by pushing the Small volume flow cells and particularly any cells locking lever back down with less than a 2 mm aperture may require use of t
84. m Installation Guide 119 7 120 LAN Communication Installation Connection and Configuration a A tlant De D q ne Agilent BOOT Service Ile ar USING Using the Agilent BootP Service Program The Agilent BootP Service program allows configuring the LAN interface card Within the Launch Manager of the program you can easily add a MAC address and IP address pair to the configuration The Launch Manager is displaying a list of the currently configured MAC address and allows modification of the current configured settings Bootp Manager xi Hardware Address HostName IP Address Subnet Mask Gatewa 0030D 30D 3277 UV Vis 10 10 10 101 8454 lab 255 255 255 0 10 10 10 1 Modify Delete Exit Manager Figure 59 Configured interfaces view of the Agilent BootP Service Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Configure the Agilent BootP Service Program If the system is on an isolated LAN and the default IP address 192 168 254 11 for the spectrophotometer is used BootP is not necessary Before configuring BootP Service you must know the IP addresses of the computer and instruments the subnet mask and the gateway The MAC or hardware address of a LAN interface is a world wide unique identifier No other network device will have the same MAC address The MAC address is printed on the card or can be read out during
85. ments such as power lines power cords etc It also gives recommendation for minimum available bench space and environmental conditions in the laboratory Unpacking Your Spectrophotometer on page 22 contains information about what to do when the packing material is damaged or the shipment is incomplete It includes a list of the items which are supplied with the instrument and computer Installing your UV Visible Spectrophotometer on page 23 gives an overview of the instrument and explains buttons indicators and the line power switch It explains where and how to connect line power communications and waste drain tubing Installing Your Computer on page 29 describes how to install the computer and peripherals e g monitor mouse keyboard and printer Because the UV Visible operating software comes already installed when the system has been ordered as a bundle this topic is covered in Chapter 6 GPIB Communication Connections and Configuration ott Agilent Technologies 17 2 Installation and Start Up e Starting the Spectrophotometer on page 30 describes the turn on procedure for the spectrophotometer and the warm up time required to fulfill all specifications e Starting the Agilent ChemStation Software on page 33 describes how you start Windows from the MS DOS operating system and how you start your Agilent ChemStation software e Operational Checkout on page 33 use this section to ensur
86. mple area is clear and wait two minutes for the lamps to ignite When you are ready to operate your spectrophotometer switch on the computer and printer V From the Windows menu install the Agilent ChemStation software For more information see Installing the Agilent ChemStation Using the DVD on page 67 V From the Windows Start menu start the Agilent ChemStation software For more information see Starting the Agilent ChemStation Software on page 33 V Ensure that your software and spectrophotometer operate properly by taking a blank measurement or by performing the automatic self test in the diagnostic section of your software For more information see Operational Checkout on page 33 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 15 1 16 Fast Installation Installing Software Only Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide ee 2 Installation and Start Up Site Requirements for Your Spectrophotometer 19 Unpacking Your Spectrophotometer 22 Installing your UV Visible Spectrophotometer 23 Installing Your Computer 29 Starting the Spectrophotometer 30 Starting the Agilent ChemStation Software 33 Operational Checkout 33 The following topics are covered in this section Site Requirements for Your Spectrophotometer on page 19 contains information about electrical require
87. mplies with Canadian ICES 001 Cet appareil ISM est conforme la norme NMB 001 du Canada South Korean Class A EMC declaration Aw 7 71 G73 34 5417 214 This equipment is Class A suitable for professional use and is for use in electromagnetic environments outside of the home o 71713 ATS A Se AA REA H a EL ALBA JIA ME A AAAA AANA ALE AS Bao w Bey clr x lo Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Safety Information A Agilent Technologies on the Internet For the latest information on products and services visit our website at http www agilent com Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 145 A Safety Information Agilent Technologies on the Internet 146 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Index A accessory boards slot 26 accurate results 36 acetic acid 43 acetone 43 acetonitrile 43 adding new instruments 74 additional qualification 33 address switch module 107 Agilent Technologies on the Internet 145 Agilent UV Visible ChemStation configuration 129 air circulation 21 air for cooling 20 ambient stray light 23 ambient temperatures 21 analytes 36 apertured cells or cuvettes 39 apertures 45 automated self test 33 baseline noise 33 benzene 43 blank 36 46 blank pushbutton 25 bootp server 118 bubble formation 44 buffer handling 126 C cable GPIB 80 car
88. new cells 41 photochemical reactions 45 photodegradation 45 photographic lens tissues 60 photosensitive substances 45 pipette 42 plastic sample cells 37 poor linearity 39 poor photometric accuracy 39 power consumption 19 power supply 19 power switch 24 Precautions 136 printer cable 29 proper operating condition 33 pushbuttons 25 pyridine 43 Q quartz sample cells 37 rapid absorbance changes 45 ready to measure 31 recommended cells 39 red blinking error condition 25 31 red error condition 24 31 Regulatory Information 143 relative humidity 21 remote connector 26 repeat ignition cycle 31 RS 232 connector 26 S safety light switches 26 sample 36 46 sample cell 36 44 sample degradation 60 sample pushbutton 25 screen element color 74 selftests 24 sensitivity 39 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide setting up an additional spectrophotometer 73 shipping container contents 22 signal to noise ratio 45 single beam instrument 36 sipper system 40 sipper sampler system 44 software certificate and registration packet 71 solute convection 44 solution 46 solution noise 44 solvent 36 Solvent Information 137 solvent suitability 43 Solvents 137 solvents 43 spectrophotometer IP addresses 117 spikes 33 stabilize 32 standard cells 48 standard pushbutton 25 standard single cell cell holder 48 Stirring 44 stirrin
89. nformation How do 1 connect Q How do I get drivers Where can I find programming samples Agilent VISA The primary VISA library Figure 49 Agilent 82357 default settings 15 Click Change Properties In the Agilent 82357 Interface Converter USB GPIB dialog window select or enter the following properties VISA interface ID to GPIBO GPIB address to 30 SICL Interface ID to hp82341 note that this is case sensitive uncheck Auto discover instruments connected to this interface see Figure 50 USB GPIB VISA interface ID GPIBO GPIB address 30 SICL interface ID ies Auto discover instruments connected to this interface Agilent 488 Properties Figure 50 Agilent 82357 interface configuration Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 99 6 GPIB Communication Connections and Configuration Configuring the Agilent 82357 USB GPIB Interface NOTE The appearance of the dialog slightly differs depending on the type of USB GPIB converter interface 16 Click OK to close the interface configuration dialog and use the File menu s Exit command to quit the Agilent Connection Expert Your GPIB library installation and 82357 interface configuration is complete 100 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Upgrading Existing 10 Libraries Upgrading 10 Libraries Follow
90. nnection 20 147 Index handling cells 42 hardware installing 64 hardware address 118 121 highest precision measurements 40 homogeneity 46 host name 126 131 HPLC grade water 44 humidity 21 CES NMB 001 144 indicator 31 indicator on front panel 24 Installation of the TCP IP protocol 112 installing hardware 64 installing the waste tubing 28 instrument icons 33 instrument types in configuration editor 119 isopropyl alcohol 43 J JetDirect card spectrophotometer IP addresses 117 K keyboard 29 L lamp intensity 32 lamps start turning on 31 LAN 115 LAN adapter JetDirect card 115 TakkToLab card 115 Lan Adapter 115 lens tissues 41 license registration number 70 148 ight beam 38 41 ine frequency 19 ine power consumption 19 ine power input socket 26 ine power supply 19 ine power switch 24 ine voltage 19 iquid samples 36 M MAC address 118 121 126 magnetic impeller 52 magnetic induction stirring 52 making measurements 36 methanol 43 methyl formate 43 MIO board slot 26 mouse 29 multicell connector 26 m xylene 43 N55011 CISPR11 143 n butyl alcohol 43 n hexane 43 non volatile memory 108 0 offline mode 33 online mode 33 operating conditions 21 optical filter 45 optical self tests 31 optical specifications of cells 38 optical surfaces 41 P parallelism 38 particulate matter 44 passivating
91. o display the Instrument Name Device Configuration To modify settings in an existing instrument configuration you must select the module first in the Selected Modules frame and press the Delete button Select the module in the Modules frame and chose the type of interfacing In case of GPIB connection select the GPIB option The GPIB address is adjusted using the scrollbar The available GPIB addresses are displayed in the box The spectrophotometer and accessories are shipped with the following default GPIB addresses GPIB Address Instrument Accessory Name 25 Spectrophotometer or 20 Temperature control accessory Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 73 5 74 Installing the UV Visible Software Press Add to add the module to the list of Selected Modules In case of a LAN connection select the LAN option and press Add In the upcoming dialog select Identify by Host Name or Identify by IP Address and enter the selected information Host Name or IP Address in the entry field Press OK to add the module to the list of Selected Modules Click OK to complete the instrument configuration If you configure more than one instrument you must ensure that each instrument has a unique GPIB or LAN address You can configure up to four spectrophotometers Click OK to enter your selections and to return to the main menu If you want to change the color of a screen element Choose Colors from the
92. ollers GB Floppy disk drives X 8 X Agilent Technologies 823574 H S IDE ATA ATAPI controllers H Keyboards 4 Mice and other pointing devices Ez E Monitors H E Network adapters EA Ports COM amp LPT E Processors Sound video and game controllers a System devices 4 Universal Serial Bus controllers Figure 54 Device Manager GPIB interfaces Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 105 6 GPIB Communication Connections and Configuration Troubleshooting 7 To configure the GPIB card please follow the instructions in Configuring the Agilent 82350 GPIB Interface on page 86 or Configuring the Agilent 82357 USB GPIB Interface on page 92 106 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Changing the GPIB Address of the Spectrophotometer This section describes how to set change the GPIB address of the spectrophotometer GPIB addresses are only applied in configurations using a GPIB connection to the spectrophotometer In a LAN connection the GPIB address is not used The connector for the interface is located on the rear panel of the spectrophotometer Next to the connector is an address and control switch module This module is shown in Figure 55 The address switches are set at the factory to a default GPIB address of 25 Gn fact the binary equivalent of 25
93. om B_02_01 SI 44 Setup exe K This file does not have a valid digital signature that verifies its x publisher You should only run software from publishers you trust w can decide what soft to run Click Run to continue 4 Read the licence agreement and click Yes to accept License Agreement Please read the following license agreement carefully Press the PAGE DOWN key to see the rest of the agreement AGILENT TECHNOLOGIES INC SOFTWARE LICENSE TERMS ATTENTION E KEAR THE SOFTWARE IS SUBJECT TO THE LICENSE TERMS SET FORTH LOW IF YOU DO NOT AGREE TO THESE LICENSE TERMS THEN A DO NOT INSTALL OR USE THE SOFTWARE AND B YOU MAY RETURN THE SOFTWARE FOR A FULL REFUND OR IF THE SOFTWARE IS SUPPLIED AS PART OF ANOTHER PRODUCT YOU MAY RETURN THE ENTIRE PRODUCT FOR A FULL REFUND Do you accept all the terms of the preceding License Agreement If you choose No the setup will clase To install Agilent UY ChemStation Rev B 02 01 you must accept this agreement InstallShield lt Back Yes No Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 69 5 70 Installing the UV Visible Software Installing the Agilent ChemStation Using the DVD 5 Accept the default installation path C Chem32 or choose a different install location then click Next InstallShield Wizard Choose Destination Location Select folder where Setup will install files amp
94. on Hazard The Lithium battery can be found on the Spectrometer Processor Main Board SPM Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Do not dispose of lithium batteries in domestic waste 140 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Safety Information A Environmental Conditions and Power Requirements Your spectrophotometer will operate within specifications at ambient temperatures of 0 to 40 C 82 to 104 F Hot Surface If you use the instrument at environmental temperatures higher than 50 C 122 F the rear of the instrument may get hot CAUTION The spectrophotometer can be shipped or stored where the ambient temperature is between 40 and 70 C 4 and 158 F Do not store ship or use your instrument under conditions where temperature fluctuations could cause condensation within the instrument Condensation will damage the electronics If your instrument was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation You can operate your spectrophotometer in environments with relative humidity up to 95 non condensing provided the temperature is between 25 40 C 77 to 104 F Avoid air circulation of varying temperatures like from refrigerators or air conditioners and exposure to direct sun light to ensure t
95. operate your instrument from a power outlet which has a ground connection Disconnecting Device Shock Hazard To disconnect the instrument from line power disconnect the power cord from the supply The power supply still uses some power and a shock hazard remains inside the instrument even if the power switch on the front panel is turned off For safety reason do not position the equipment so that it is difficult to operate the disconnecting device Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 139 A Safety Information Allowing Hot Instrument to Cool Hot Surface The lamps and its surroundings remain hot for up to five minutes after the lamp is turned off To avoid burns allow the lamp compartment to cool before carrying our any work in this area UV Light Source Eye Hazard Eye damage may result from directly viewing light produced by deuterium lamps Always turn off the deuterium lamps before opening the lamp door on the instrument When operating the instrument always wear safety glasses prescribed for viewing ultraviolet rays Direct viewing of ultraviolet rays without protection causes permanent blindness or vision impairment Power Cord Lithium Do not replace the mains power cord with one that is inadequately rated When replacing the mains power cord use only a mains power cord with a rating equivalent to the one supplied with the instrument Batteries Information Explosi
96. pply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Safety Information A Solvent Information CAUTION Organic and aqueous solvents can be hazardous if handled improperly Always follow the precautions listed below to protect both operator and instrument Flow Cells Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of flow cells Solvents Always filter solvents small particles can permanently block capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your analysis method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls 02 gt 2COCl 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Analysis grade ethers which can contain peroxides for example THF dioxane di isopropylether
97. rd must be installed the spectrophotometer must be connected to the PC and a bootp server must be installed and configured before the spectrophotometer s can be used See Installing and Configuring TCP IP on the PC on page 112 for details Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 127 7 LAN Communication Installation Connection and Configuration Configuring an Agilent Cary 8454 UV Visible Spectrophotometer This section describes how to configure an Agilent Cary 8454 UV Visible spectrophotometer for LAN communication 1 Start the Agilent ChemStation Configuration Editor by double clicking on the Configuration Editor icon in the UV Visible ChemStation start up group by choosing Start gt All Programs gt UV Visible ChemStations gt UV Vis Configuration Editor 2 Select the Configure gt Instruments menu option 3 Select the Instrument Type UVVis enter an Instrument Name for your configuration e g 8454 UV Visible System 1 choose an Initial Screen Window Size and click OK Select Instrument Instrument Type Instrument Name 8454 UY Yisible System Initially Start Instrument Session O Yes No Initial Screen Window Size Icon Full screen Figure 62 Instrument configuration dialog 128 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 4 If GPIB communication has been setup for
98. ries Suite l iComponents Directory C Program Files IV VISA Directory C Program Files VISA Selected Features Agilent VISA as primary Manuals Examples lt InstallShield Figure 28 Agilent 10 Libraries Suite installation summary 84 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Installation of the Agilent GPIB Communication Library 6 A window with feature explanations and the progress window below will be displayed Agilent IO Libraries Suite 15 0 Insta C Agilent TMFramework Connectivity AceApplicationBase d Figure 29 Agilent 10 Libraries Suite installation progress 7 Click Finish to complete the setup i0 Agi braries Suite 15 0 Agilent IO Libraries Suite 15 0 has been connectivity solutions successfully installed 2 1 0 Hardware Run Connection Expert A Agilent VEE Pro BEJO O 352M A ts Agilent Open ee Agilent Technologies Agilent Technologies Inc 1963 2007 Figure 30 Agilent 10 Libraries Suite completion dialog As a result of your installation you should see the IO library icon on the Windows task bar ES cl 3 48 pm Figure 31 Agilent 10 Libraries Suite quick launch icon Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 85 6 GPIB Communication Connections and Configuration Configuring the Agilent 82350 GPIB Interface e To configure your 8235
99. s the cell from contamination by tissue particles or rinse the cell with blank or sample solution Floating particles in the cell will deflect the light beam and so lead to a very poor quality of the measured spectrum a floating particles will deflect and scatter the light beam lt q Figure 9 Floating particles in a cell Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 41 3 Good Measurement Practices CAUTION 0 01 0 00 0 01 0 02 0 03 Absorbance 0 04 0 05 200 400 600 800 1000 Wavelength nm Figure 10 Spectrum taken with floating particles in the light path Lens tissues for glasses or other uses often contain detergents or lubricants which can affect your measurements If possible avoid cleaning the faces of your cell between blank and sample measurements Handling Cells Always install a cell so that it faces the same direction to minimize problems with cell non uniformity For best results with microcuvettes leave your sample cell clamped in position throughout the measurement sequence Solutions should be removed and replaced by pipette or use flow cells If glass pasteur pipettes are used make sure that the optical windows of the cell are not touched or scratched by the pipette 42 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Solvents Good Measurement Practices 3 Your choice of solvents should be based primarily on the
100. se of the instrument on the LAN the G1369C LAN card used to connect the spectrophotometer to the LAN does not store any settings so each time the instrument is powered on the card sends a request to the network for an IP address and setting information The implementation of the BootP protocol on the G1369C LAN card conforms to RFC 951 and RFC 1048 In order to provide this information to the card the BootP Service must be operational before the card sends the request and the BootP Service must be configured to know the hardware address also called Media Access Control acronym MAC address of the LAN interface card Multiple LAN interface cards can be configured by means of a single BootP Service If your network is already using a BootP Service please refer to section Configuring the G1369C LAN Card Using BootP on page 125 If you do not have a BootP Service we suggest to use the Agilent BootP Service program supplied on the Agilent UV Visible ChemStation DVD Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Addresses Before installing and configuring BootP Service you must know the IP addresses of the computer and instruments the subnet mask and the gateway Table 7 Example Device Address PC 10 10 10 1 Spectrophotometer 10 10 10 101 Subnet mask 255 255 255 0 Gateway 10 10 10 1 Agilent Cary 8454 UV Visible Spectroscopy Syste
101. stem Installation Guide 75 5 Installing the UV Visible Software Installing the Agilent ChemStation Using the DVD 76 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide eec 6 GPIB Communication Connections and Configuration GPIB Communication 78 Installing an GPIB Interface 79 Installation of the Agilent GPIB Communication Library 82 Configuring the Agilent 82350 GPIB Interface 86 Configuring the Agilent 82357 USB GPIB Interface 92 Upgrading Existing 10 Libraries 101 Working with your Agilent I0 Libraries 102 Troubleshooting 103 This chapter describes how to install the GPIB communication library and configure the GPIB interface of your computer and how to change the GPIB address of your spectrophotometer Additional information about the installed I O library the use of the tools and hints for troubleshooting are provided e Changing of the GPIB address might be required when you run more than one module connected to your GPIB interface The GPIB address of the connected modules must be unique ot Agilent Technologies 77 GPIB Communication Connections and Configuration GPIB Communication 78 IEEE 488 is a short range digital communications bus specification Originally created for use with automated test equipment the standard is still in wide use for that purpose IEEE 488 is also commonly known as HP IB Hewlett Packard
102. t MAC Address Host Name IP Address Comment if desired Subnet Mask Gateway Address 6 Click OK 7 Exit Launch Manager and power cycle both the PC and the spectrophotometer 8 Ping the IP address from a command prompt on the PC to verify Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide LAN Communication Installation Connection and Configuration 7 Using the Agilent BootP Service Configure the Agilent BootP Service BootP Service starts automatically when your PC reboots In order to add an additional instrument or to change the configuration the service must be stopped After the changes have been applied the service must be restarted 1 To stop the BootP service go to Start gt Settings gt Control Panel gt System and Security and select Administrative Tools gt Services The Services window appears u Services Eile Action Yiew Help e gt m 2 eq Services Local Services Local Select an item to view its Agilent Bootp Service description amp Agilent Event Distributor Sa Alerter S Application Layer Gateway Service amp Application Management amp ASP NET State Service amp Automatic Updates Background Intelligent Transfer Service sy ClipBook Sa COM Event System Sa COM System Application amp Computer Browser amp Cryptographic Services DHCP Client Distributed Link Tracking Client Distributed Transaction Coordinator DNS Client Error Reporting Service 3 ui Ext
103. t 20 seconds before the lamps start turning on Under cold ambient conditions the lamp may not ignite and the instrument will repeat the ignition cycle automatically The entire turn on and self test process will take about 1 2 minutes Optical self tests are performed which last a few seconds 5 When the green indicator at the upper left corner of the front panel comes on the instrument is ready to measure and you can turn on your computer and printer If the software is set to leave both lamps off at turn on of the spectrophotometer the front panel indicator remains yellow If the indicator shows a red color there is an error condition Try turning on the spectrophotometer again red error condition that is the spectrophotometer does not pass one of the self tests In this case turn on your computer and start the UV Visible operating software The software will give a detailed error message and possible explanations are in the online help system Additionally the Operator s Manual gives information about troubleshooting red blinking error condition of the spectrophotometer processor system Because in this case there is no communication with the computer there will be no error message The online help system and the Operator s Manual gives more information about troubleshooting Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 31 2 32 Installation and Start Up Instrument Warm up Technically on
104. t ChemStation Security Pack for UV Visible Spectroscopy User s Guide for details During the installation some of your configuration files will be changed If you don t intend to use any ChemStation software you can remove the ChemStation ini file located in the operating system folder C Windows by default Installation Procedure Use the following procedure if you are using the DVD to install or upgrade the Agilent ChemStation Before installing the software e Set up the computer hardware If GPIB communication will be used connect as described in Installing an GPIB Interface on page 79 not required for LAN communication e Make sure an operating system supported by your revision of the Agilent UV Visible ChemStation software is available on the target PC 1 Insert the Agilent UV Visible ChemStation Products DVD into the DVD drive 2 From the Start menu in the taskbar select Start gt Run 3 Atthe command line type diskdrive Setup exe for example D Setup exe then click OK 68 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing the UV Visible Software 5 Installing the Agilent ChemStation Using the DVD NOTE Using Windows 7 you may get the warning dialog below several times during the installation The publisher could not be verified Are you sure you want to tun this software m Name B_02_01 SI 44 Setup exe Publisher Unknown Publisher Type Application Fr
105. t is more easily controlled Water temperature can be up to 100 C 212 F with a maximum allowable back pressure of 4 bar approximately 60 psi If air is used to drive the cell stirring module the pressure should be less than 0 3 bar approximately 5 psi when the stirrer is started Again the maximum pressure is 4 bar approximately 60 psi There are several ways you can connect a water or air supply to the cell stirring module see Figure 16 In all cases we recommend using 3 16 inch i d silicon tubing between the water supply and the stirring module The stirring module can be used alone without the thermostattable functions of the cell holder e The same water supply can be used to regulate sample cell temperature and to power the cell stirring module Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installing Accessories 4 If you need a faster stirring speed than can be obtained by connecting the manifold and cell stirring module in series you can connect separate supplies to the manifold and stirring module Figure 16 Tubing connections for a cell stirring module Table 2 Tubing connections for a cell stirring module Connection Comment A in B out Without stirring C in D out Without cell thermostatting A in B to D C out In series with cell thermostatting A and C in B and D out In parallel with cell thermostatting Agilent Cary 8454 UV Visible Spectroscopy System
106. talling the UV Visible Software 5 Installing the Agilent ChemStation Using the DVD Overview This section explains how to use the Agilent Technologies UV Visible ChemStation Software Products DVD to e install the Agilent ChemStation for the first time e upgrade repair an existing Agilent ChemStation software e install an additional Agilent ChemStation module Agilent UV Vis ChemStation B 05 02 and later versions are only supported on Microsoft Windows 7 Professional and Enterprise 32 Bit and 64 Bit SP1 Microsoft Server 2003 and Microsoft Server 2008 32 bit SP1 Local Administrator rights on the target PC are required to perform the installation Uninstalling Pre existing Agilent UV Visible ChemStation Software To remove all analytical ChemStation software from your hard disk 1 Insert your Agilent Technologies UV Visible ChemStation Software Products DVD into the drive 2 Run the setup program on the DVD 3 Select Remove and then click Next Note that the removal of ChemStation software can also be done from within the Control Panel Only files installed by the product DVD are removed All additional files such as method and data files are not automatically removed By default they are located in the respective instrument directory C Chem32 1 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 67 5 Installing the UV Visible Software If the Security Pack had been installed please see your manual Agilen
107. te the registry and add a new Program Group called Agilent IO Libraries Suite Windows 7 may request your permission to continue Press the Run button to continue Open File Security Warning E The publisher could not be verified Are you sure you want to tun this software E i Name YAB_02_01SL44 Setup exe L Publisher Unknown Publisher Type Application From B_02_01 SI 44 Setup exe publisher You should only run software from publishers you trust x This file does not have a valid digital signature that verifies its How can decide what software to run Figure 24 Windows security warning 82 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Installation of the Agilent GPIB Communication Library The InstallShield Wizard prepares for the installation and displays its Welcome dialog Agilent IO Libraries Suite 15 0 InstallShield Wizard Welcome to the InstallShield Wizard for Agilent 10 Libraries Suite 15 0 The InstallShield Wizard will install Agilent 10 Libraries Suite 15 0 on your computer To continue click Next Figure 25 Agilent 10 Libraries Suite installation Welcome screen 3 Click Next to continue and select I accept the terms of the license agreement 0 InstallShield Wizard License Agreement Please read the following license agreement carefully AGILENT TECHNOLOGIES INC SOFTWARE LICEN
108. th grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of su
109. the installation procedure described Installing New IO Libraries on page 82 The existing library will be automatically removed Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 101 GPIB Communication Connections and Configuration Working with your Agilent 10 Libraries Working with your Agilent 10 Libraries 102 For details about the Agilent IO Libraries Suite please refer to the additional documentation provided To access the documentation click on the IO Icon R sis in the notification area of the Taskbar choose Documentation and select the type of information you need see Figure 51 Agilent VISA Agilent Connection Expert Utilities Agilent VISA Options Connectivity Guide Documentation Interface Manuals gt Help Topics LXI Event and Timing Guide Installation Information SICL Users Guide 10 Config Information VISA Users Guide Hide Agilent IO Control 10 Libraries Suite Readme Exit About Agilent IO Control EN ka RY 11 10AM Figure 51 Agilent 10 Libraries Suite documentation access Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide GPIB Communication Connections and Configuration 6 Troubleshooting Missing files during installation It may happen that some IO installation files are not copied during the installation process In this case use the repair option of the Agilent IO Libraries Suite as follows 1 Start the Control Panel s Add
110. the spectrophotometer the following screen will come up 25 is the default GPIB address 8454 UY Visible System 1 Device Configuration UVVis Modules GPIB CLAN Spectrophotometer 8452A GPIB Address jo 8454 Temp Control Selected Modules Spectrophotometer 8454 GPIB 25 Figure 63 Device configuration dialog GPIB interfacing Select the Spectrophotometer 8454 GPIB 25 entry in the Selected Modules tab and click Delete to delete the selected module 5 Select the Spectrophotometer 8454 in the Modules tab select the LAN option and click Add to add to the Selected Modules box 6 Select Identify by IP Address and type in the IP address of the LAN interface card If a DNS server is installed you can also define the host name Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 129 7 LAN Communication Installation Connection and Configuration 7 Click OK to exit and to return to the Device Configuration dialog box which now should look like this 8454 UV Visible System 1 Device Configuration UYVis Modules C GPIB LAN GPIB Address p 4 Spectrophotometer 8452A Temp Control Selected Modules Spectrophotometer 8454 IP 16 16 16 161 Figure 64 Device configuration dialog LAN interfacing 8 Click OK to exit the Device Configuration dialog box and save your configuration changes by selecting File gt Save
111. tional bath in a multi bath dissolution system G1118AA In all cases an appropriate amount of virtual memory should be configured A maximum of four instruments may be interfaced to each Agilent ChemStation If you are upgrading from a previous version of Agilent ChemStation you must uninstall the old version of ChemStation first Scheduled Processes Some scheduled processes like virus checking or defragmenting may cause instrument communication problems Due to these problems any automated measurement will be aborted To prevent problems of this kind please disable any scheduled event Number Format To avoid wrong values displayed or printed the International settings must include the correct number format Set the Number Format Decimal Separator to decimal point in Control Panel gt Region and Settings Printer Please make sure that a default printer is configured in your Microsoft Windows operating system This is done via the Control Panel application Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 65 5 Installing the UV Visible Software Agilent ChemStation Computer Details If no default printer is configured you may encounter the following problems e the Copy To Clipboard menu may have an error e in the Custom Report View there may be problems with new templates e the Print Preview will not work 66 Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Ins
112. to reduce unwanted absorbance from the water If you are using the sipper sampler system the water should be degassed to avoid bubble formation in the flow cell especially if the water comes from a pressurized water supply Sample Preparation 44 The sample cell should be rinsed three to five times with your intended solvent before you fill it with the pure solvent that will be used in the measurement Turning the cell upside down on a small stack of absorbent tissues will help remove any residual solvent This treatment will minimize contamination from previous experiments Samples which contain colloidal dispersions dust or other particulate matter should be filtered centrifuged or allowed to settle If not the overall attenuation of transmittance spectrum due to light scattering and or reflection will hide the spectral information from the analyte Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Good Measurement Practices 3 Photosensitive Samples A few substances are very photosensitive They degrade or undergo photochemical reactions if exposed to light This can be easily seen by a decrease of sample absorbance over time Use of Filters The shorter wavelength higher energy UV light is most likely to degrade photosensitive samples If you have a problem you can selectively block portions of the UV spectrum with a UV cut off filter An optical filter wheel assembly with three cut off filters is available
113. to the spectrophotometer can be assigned by using the Agilent bootp service This service assigns the LAN interface card with a configured IP address each time an IP address is requested Refer to the next section Using the Agilent BootP Service on page 118 If the system is on an isolated LAN and the default IP address 192 168 254 11 for the spectrophotometer is used BootP is not necessary Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide 117 7 LAN Communication Installation Connection and Configuration Using the Agilent BootP Service 118 What is the Agilent BootP Service If the system is on an isolated LAN and the default IP address 192 168 254 11 for the spectrophotometer is used BootP is not necessary The Agilent Bootstrap Protocol Service acronym BootP Service provides central administration of IP addresses for Agilent instruments on a LAN The service runs on the instrument LAN PC which must be running TCP IP network protocol and cannot run a DHCP server service or another BootP Service When an instrument is powered on an Agilent LAN interface card in the instrument broadcasts a request for an IP address or Host Name and provides its hardware address as an identifier The request may continue for up to 5 minutes The Agilent BootP Service answers this request and passes a previously defined IP address and Host Name associated with the hardware address to the requesting instrument In ca
114. trophotometer on page 30 V Install the Agilent ChemStation software For more information see Installing the Agilent ChemStation Using the DVD on page 67 V From the Windows Start menu start the Agilent ChemStation software For more information see Starting the Agilent ChemStation Software on page 33 V Ensure that your software and spectrophotometer operate properly by taking a blank measurement or performing the automatic self test in the diagnostic section of your software For more information see Operational Checkout on page 33 V If you need information about solvents sample preparation blank measurements cuvette handling or how to insert a cuvette see Chapter 3 Good Measurement Practices V Install any accessories For simple accessories see Chapter 4 Installing Accessories for complex accessories see the instructions supplied with the accessory or the Installation and Maintenance section of the Agilent ChemStation online help Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Fast Installation 1 Installing Software Only V Unpack the software check for damage and check the contents against the packing list V Ensure that your computer and printer meet the requirements for proper operation of the software For more information see Minimum PC Requirements for Agilent ChemStation on page 64 V Switch on the spectrophotometer make sure the sa
115. ximately 50 cm 19 7 inches wide and 60 cm 23 6 inches deep Additional space is required for accessories like pumps and autosamplers Agilent Cary 8454 UV Visible Spectroscopy System Installation Guide Installation and StartUp 2 Environment Your spectrophotometer will operate within specifications at ambient temperatures of 0 to 40 C 82 to 104 F If you use the instrument at environmental temperatures higher than 50 C 122 F the rear of the instrument may get hot The spectrophotometer can be shipped or stored where the ambient temperature is between 40 and 70 C 4 and 158 F Do not store ship or use your instrument under conditions where temperature fluctuations could cause condensation within the instrument Condensation will damage the electronics If your instrument was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation You can operate your spectrophotometer in environments with relative humidity up to 95 non condensing provided the temperature is between 25 and 40 C 77 to 104 F Avoid air circulation of varying temperatures like from refrigerators or air conditioners and exposure to direct sun light to ensure the instrument operates within performance specifications Your spectrophotometer can be stored at altitudes up to 4 600 m 14 950 ft and operated at altitudes up to 2 000 m 6 500 ft Agilent Cary 8454 UV Visible

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