Ettan DALTsix Large Vertical Electrophoresis System
Contents
1. Fig 2 Brussel sprout Brassica oleracea var Gemnifera extract separated in the first dimension on an Immobiline DryStrip 3 10 NL 24 cm The second dimension was separated on the Ettan DALTsix with a 12 5 T 2 7 C polyacrylamide gel using the Laemmli buffer system Amersham Biosciences 2 D electrophoresis The compact 6 gel Ettan DALTs x Electrophoresis Unit is simple to assemble and use Only four parts are needed to assemble an Ettan DALTsix Electrophoresis Unit See Fig 4 1 the lid with anode and cathode leads and cathode electrode 2 the upper buffer chamber Fig 3 3 anode assembly cassette carrier and 4 lower buffer chamber with built in ceramic heat exchanger and buffer circulation pump see Fig 4 Connecting the heat exchanger to a MultiTemp III Thermostatic Circulator is quick and mess free through the QuickFit connectors pre assembled on the heat exchanger tubing Parallel strands of platinum wire installed in the lid and the anode assembly cassette carrier control the uniformity of the electrical field A set of distribution ports on the lower edge of the anode assembly cassette carrier direct buffer circulation around and between the cassettes for efficient heat control Buffer circulates into the lower buffer chamber on the right hand side up between the cassettes down the left hand side and across the ceramic heat exchanger Assembling the system for an electrophoresis run starts by i2. T Cassette Removal Tool seet x x x 2 pk 80 6474 82 Only needed when spot picking lab cast gels and when background fluorescence is not a concern Included with caster Included with main instrument p3 2 D electrophoresis Specifications Ordering information Ettan DALTs x Electrophoresis Unit product code no Gel capacity 6 gels Ettan DALTsix Electrophoresis Unit 115 V 80 6485 08 Electrophoresis buffer volume 4 5 anode 0 8 cathode Includes Two pieces 2 m long vinyl tubing with one Circulation pure voltage 115 V 60 Hz or 220 V 50 Hz pair of oe Connectors 1 CRESE removal tool Bufer eireulstion pump raie 10 Vmin a DALT six Electrophoresis Unit 220 V 80 6485 27 ncludes Two pieces 2 m long vinyl tubing with one Cooling Ceramic heat exchanger pair of QuickFit Connectors 1 Cassette removal tool Pressure limit ceramic heat exchanger 87 kPa 12 psi Ettan DALT Gel Casting Cassette 1 0 mm 80 6466 84 Maximum temperature 40 C Ettan DALT Gel Casting Cassette 1 5 mm 80 6488 69 Environmental operating conditions Indoor use 4 40 C Ettan DALT Pre cast Gel Cassette 80 6466 65 ballad a Ettan DALT LF Glass Plate Set 80 6475 58 gory Includes 2 borosilicate glass plates with attached spacers ae Me ETesIee 2 Ettan DALT Glass Plate Set 80 6475 39 Safety features Safety interlock lid submersi
3. ble pump Includes 2 glass plates with attached spacers Product and safety certifications EN61010 1 UL3101 1 CSA22 2 Wonder Wedge 80 6127 88 TOO e EMO ENGI AOK CE Marked Ettan DALT Blank Cassette Insert 80 6467 03 ANATOR A wee AAEE L Ettan DALT Gel Caster 80 6485 46 Weight 12 2 kg Includes 7 Separator Sheets 0 5 mm and 6 Filler Sheets 1 0 mm Order cassettes separately Ettan DALTs x Gel Caster Ettan DALT Separator Sheets 0 5 mm 16 pk 80 6467 41 Gel Capacity 6 gels 1 0 mm or 1 5 mm thick Ettan DALT Filler Sheets 1 0 mm 6 pk 80 6467 60 Volume total acrylamide solution 425 ml for 1 0 mm thick gels 550 ml Ettan DALT Gradient Maker 80 6487 36 for 1 5 mm thick gels Equilibration Tube Set 12 pk 80 6467 79 Dimensions h x w x d 27 x 37 x 8 cm Ettan DALT Cassette Removal Tool 2 pk 80 6474 82 Weight 3 6 kg Ettan DALT Cassette Rack 2 pk 80 6467 98 1 0 mm Gel Casting Cassettes poets stainless steel tray 23 x 42 cm a Cassette dimensions w x h x d 27 6 x 21 7 cm x 7 mm 2 stainless steel trays and 1 clear plastic lid Slab gel dimensions w x h x d 25 5 x 20 5 cm x 1 0 mm Roller 80 1106 79 Ettan DALT Gel 12 5 26 x 20 cm 6 pk 17 6002 36 1 5 mm Gel Casting Cassettes Ettan DALT Buffer Kit for 12 pre cast gels 17 6002 50 Cassette dimensions w x h x d ei oe 21 7 X 7 5 mm EPS 601 Power Supply 6 600 V 1 400 mA 1 100 W 18 1130 02 Slab gel dimensions w x h x d 25 5 x 20 5 x 1 5 mm MultiTemp IIl Thermostatic Circulator 115 V 18 1102 77 MultiTem
4. cassette See Fig 6 Glass Plate Set 80 6475 39 is used for lab casting 1 0 mm thick gels that will be used for spot picking The set includes one short glass plate and one long glass plate with spacers attached These plates are not hinged LF Glass Plate Set 80 6475 58 is used for lab casting 1 0 mm thick gels for fluorescence scanning applications such as 2 D Difference Gel Electrophoresis DIGE No more handling of acrylamide solutions is required when using large format 26 x 20 cm pre cast Ettan DALT Gel 12 5 for second dimension separations The polyacrylamide gel is bound to a plastic support film which eases handling and assures dimen sional stability The Ettan DALT Buffer Kit for the pre cast gels includes concentrated buffers and agarose sealing solution Ettan DALT Gel 12 5 and Ettan DALT Buffer Kit use a patented buffer formulation for long storage stability and high reproducibility Fig 7 Plastic backed Ettan DALT pre cast gel loaded into pre cast gel cassette The cassette is designed for easy application of the IPG strip and agarose sealing solution Multiple gel staining with Ettan Staining Tray Set Large format second dimension gels can be conveniently stained in the Ettan Staining Tray Set For high sensitivity and reliable detection of proteins we recommend the PlusOne Silver Staining Kit Protein Fig 8 PlusOne Silver Staining Kit and Ettan DALTtwelv
5. e Staining Tray Set The set includes 1 perforated stainless steel tray 23 x 42 cm 2 stainless steel trays and 1 clear plastic lid Table 1 Ettan DALTs x System ordering guide needed with needed with needed for product lab cast gels pre cast gels 2 D DIGE Ettan DALTs x Electrophoresis Unit x x x PlusOne equilibration chemicals Tris Urea Glycerol SDS DTT Bromophenol Blue Equilibration Tube Set 12 pk 80 6467 79 Ettan DALTsix Gel Caster 80 6485 46 Ettan DALTsix Gradient Maker 80 6487 36 PlusOne gel casting chemicals and buffers Acrylamide Bisacrylamide TEMED Ammonium persulfate Tris Glycine x x x x x x 1x x Ettan DALT Gel Casting Cassette x 1 0 mm 80 6466 84 Ettan DALT Gel Casting Cassette x 1 5 mm 80 6488 69 Ettan DALT LF Glass Plate Set 80 6475 58 x x Ettan DALT Glass Plate Set 80 647 5 39 see Ettan DALT Pre cast Gel Cassette 80 6466 65 Roller 80 1106 79 Ettan DALT Gel 12 5 6 pk 17 6002 36 Ettan DALT Buffer Kit 17 6002 50 Ettan DALT Blank Cassette Insert 80 6467 03 Wonder Wedge 80 6127 88 Ettan DALT Cassette Rack 2 pk 80 6467 98 PlusOne staining chemicals Silver Coomassie x X X x x xK xK xK Ix Ettan Staining Tray Set 80 6468 17 x X X x x x Ettan DALT Separator Sheets see 0 5 mm 16 pk 80 6467 41 Ettan DALT Filler Sheets seet x x 1 0 mm 6 pk 80 6467 60 Ettan DAL
6. file 2 D electrophoresis Ettan DALTs x Large Vertical Electrophoresis System for second dimension 2 D electrophoresis Ettan DALTsix Electrophoresis System for large format and medium throughput 2 D electrophoresis e Runs up to 6 gels 26 cm wide by 20 cm long each with 18 or 24 cm long Immobiline DryStrip gels e Accommodates lab cast and pre cast gels e Simple to use 1 0 mm or 1 5 mm pre assembled hinged gel cassettes e Innovative anode assembly cassette carrier and upper buffer chamber simplify loading and unloading e Built in ceramic heat exchanger and buffer circulation pump ensure efficient cooling e Durable reliable and flexible The Ettan DALTsix Electrophoresis Unit runs up to six large format 26 x 20 cm second dimension acrylamide gels in just 4 5 hours The gels accept the first dimension 18 or 24 cm Immobiline DryStrip gels IPG strips from both IPGphor and Multiphor II isoelectric focusing systems True to its heritage Ettan DALTsix uses the same convenient pre assembled gel cassette designs with hinged plates and permanent spacers as the original Amersham Biosciences DALT and Ettan DALTtwelve DALT II Systems For lab casting choose a 1 0 mm or 1 5 mm gel casting cassette and high quality PlusOne gel casting chemicals For precast gels on support film choose the Ettan DALT Gel 12 5 1 0 mm precast gel cassette For some fluorescence applications low fluorescence borosilicate gla
7. nserting the anode assembly cassette carrier into the lower buffer chamber and the anode buffer Then turn on the buffer circulation pump and MultiTemp III Insert up to 6 filled gel cassettes into the slots in the cassette carrier When you run fewer than six gels fill remaining slots with blank cassette inserts Fig 3 Upper Buffer Chamber with slots for 6 cassettes The upper buffer chamber has six gel slots and a center handle slot each flanked by a double rubber gasket to seal the cassettes in place See Fig 3 The buffer chamber simply slides down over the tops of the gel cassettes and blank inserts Add cathode buffer to the upper buffer chamber check that buffer levels are equal set the safety lid in place connect the power supply and start the electrophoresis run The lid has an integral safety interlock that disconnects the power to the electrodes if the lid is opened during the run When the electrophoresis run is complete remove the lid Lift out the cassette carrier Remove the upper buffer chamber and slide the gel cassettes from the slots Open them with a Wonder Wedge tool df 80 6490 02 p2 lid with leads TLL safety interlock upper buffer chamber anode assembly cassette carrier lower buffer chamber ceramic heat exchanger buffer circulation pump Fig 4 Exploded view of Ettan DALTsix Electrophoresis Unit Ettan DALTs x Gel Caster and Gradient Maker Ettan DALTsix Gel Ca
8. p IIl Thermostatic Circulator 220 V 18 1102 78 Ettan DALTs x Gradient Maker PlusOne equilibration gel casting and staining chemicals Volume 1000 ml buffers See BioDirectory Catalogue Weight 0 73 kg Handbook 2 D Electrophoresis Using Immobilized 80 6429 60 This declaration of conformity is only valid for the instrument when it is e used in laboratory locations e used as delivered from Amersham Biosciences except for alterations described in the User Manual and e connected to other CE labeled instruments or products recommended or approved by Amersham Biosciences www amershambiosciences com PH Gradients Principles amp Methods Asia Pacific 852 2811 8693 Australasia 61 2 9899 0999 Austria 01 576 0616 24 Belgium 0800 73888 Canada 1 800 463 5800 Central Eastern amp Southeastern Europe 43 1 982 3826 Denmark 4516 2400 Finland 358 0 9 512 3940 France 0169 35 67 OO Germany 0761 4903 402 Italy 02 27322 1 Japan 81 3 5331 9336 Latin America 55 11 3667 5700 Middle East and Africa 30 1 96 OO 687 Netherlands 0165 580 410 Norway 23 18 58 OO Portugal 21 417 7035 Russia amp other C I S amp N I S 7 095 232 0250 Southeast Asia 60 3 724 2080 Spain 93 594 49 50 Sweden 018 612 19 OO Switzerland O1 802 81 50 U K 0800 616 928 USA 1 800 526 3593 Ettan Immobiline PGphor Multiphor MultiTtemp and PlusOne are trademarks of Amersham Biosciences group Amersham Biosciences Amersham Place Little Chalfon
9. ss plates and vinyl spacers are available Loading gel cassettes into the unit is simple Slide up to six filled gel cassettes into slots on the anode assembly cassette carrier then lower the carrier into the lower buffer chamber The upper buffer chamber then slides down over the cassettes sealing the upper ends of the cassettes into the chamber Efficient cooling and close temperature control in the Ettan DALTsix Electrophoresis Unit are maintained by the combination of the built in ceramic heat exchanger and buffer circulation pump with an external MultiTemp III thermostatic circulator Ettan DALTsix system is modular in design The system components include the Ettan DALTsix Electrophoresis Unit Gel Caster Gradient Maker an EPS 601 Power Supply and MultiTemp III Thermostatic Circulator Ettan 80 6490 02 Rev AA 10 01 pl Fig 1 The compact 6 gel capacity Ettan DALTsix Electrophoresis Unit DALTtwelve Electrophoresis System accessories including blank cassette insert separator sheets filler sheets cassette rack and staining tray set are fully compatible with Ettan DALTszx units Ettan DALTsix System is the newest member of a long line of innovative durable and reliable vertical electro phoresis units building on more than 30 years of experience in the field pH 3 10 NL _____ __ gt h T u a i ae ia _ i AE a rr HA x Pi i a Ems i opin He r ll p
10. ster holds up to six 1 0 mm or 1 5 mm cassettes with separator sheets for casting homogeneous or gradient gels If fewer gels are to be cast the unused volume is filled with blank cassette inserts and filler sheets Pouring gel solution into the filling groove in the back wall of the caster fills the cassettes from below to produce consistently uniform gels The Ettan DALTsix Gradient Maker is designed to produce linear convex and concave exponential gradients of solutions ranging in volume from 200 1000 ml A sliding valve at the bottom quickly connects or disconnects the chambers _ _ Fig 5 Ettan DALTs x Gel Caster for casting 1 0 and 1 5 mm large format gels 2 D electrophoresis Fig 6 Pre assembled hinged Gel Casting Cassette for 26 x 20 cm lab cast gels The differential plate heights ease IPG strip loading The two glass plates are joined along the left edge by a silicone rubber hinge Sticky vinyl spacers 1 0 or 1 5 mm thick are glued on each edge of the long plate Close and open the cassette like a book Choose large format lab cast or pre cast gels for Ettan DALT six runs The large format gels are 26 cm wide by 20 cm long to accept both 18 or 24 cm IPG strips Your choices for the second dimen sion gel include casting your own 1 0 or 1 5 mm thick gels and using 1 mm thick pre cast Ettan DALT Gel 12 5 For regular lab cast gels we recommend the pre assembled hinged gel casting
11. t Buckinghamshire England HP7 9NA Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Munzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences 654 Minnesota Street San Francisco CA 94107 USA Coomassie is a trademark of ICI plc Amersham and Amersham Biosciences are trademarks of Amersham plc 2001 Amersham Biosciences Corp All rights reserved All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Printed in the USA Amersham Biosciences
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