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INSTRUCTION MANUAL System User`s Guide UV

1. Check Description Part No Qty O Spectrophotometer 206 25201 91 1 O AC Power Cable 071 60816 12 1 6 UV 1800 Instruction Manual System User s 206 97040 1 Guide This manual O UV 1800 Instruction Manual Operation 206 97042 1 Guide UVProve Software Install CD 206 21439 91 1 UVProve Tutorial Instruction Manual 206 94459 1 D Shimadzu User Authentication Tool 223 10410 1 Instruction Manual 1 2 WE UV 1800 SERIES HE Components 1 2 1 UV 1800 Main Body Front and Top Views oo 0 0 O O O O p D 0000 o 00000 000000 Fig 1 2 Front and Top Views of UV 1800 No Name Description O LCD This displays the operation menus and measurement results O Keypad This is the input component for giving operation commands and numeric values to the instrument etc IS 1 2 5 Keypad Sample Compartment Open close this cover when setting the measured sample Cover US 1 2 4 Sample Compartment O Sample Compartment These are screws for fastening the sample compartment unit Set Screws IS 4 2 Removing Installing the Sample Compartment Unit Standard 6 LED This lights when the power to the unit is ON O Light Source This is the cover of the light source compartment Open close this cover Compartment Cover when replacing the light source lamp IS 3 4 Replacing the Light Source
2. A UV 1800 SERIES IE 1 3 1 2 Components 1 2 2 UV 1800 Main Body Left Side View i i 100 120 220 240V 50 60Hz 140VA AC IN E 00 JO o o 2 6 W I W I o o al Fig 1 3 Left Side View of UV 1800 No Name Description O USB Connector You can use these connecters for any of printer or USB memory stick You can also connect special accessories described below In such cases however it is necessary to purchase separately and connect the designated USB conversion adaptor to each accessory e 6 cell electronic temperature control cell positioner CPS 240A B gt USB adaptor for CPS P N 206 25234 91 e Auto sample changer ASC 5 gt USB adaptor for ASC P N 206 25235 91 For installation and connection procedures refer to the instruction manual of each accessory USB Connector for PC This is the connector for connecting PC during external control operation US Operation Guide 17 1 Connecting to a PC Optional Unit Connector This is the connector to connect the Sipper 160 or Syringe sipper special accessories For installation and connection procedures refer to the instruction manual of each accessory AC Power Connector Connect the enclosed AC power cable to the supply power from an AC electrical outlet ISS 2 2 Connecting Power 1 4 ES UV 1800 SERIES HE 1 2 Co
3. For the range of ultraviolet ray and visible lights a unit called nm nanometer is used Wavelength A Wavelength A Fig 6 2 Wavelength Generally rays of various wavelengths are mixed in the light emitted from a light source although some emit rays of specific wavelength such as laser light source or others emit light of several specific wavelengths such as mercury lamps The light of a certain wavelength extracted selectively by the use of a monochromator is called monochromatic light Light that includes all the rays in the wavelength range of visible rays is called white light The relation between wavelength and color is as follows Ultraviolet Greenish blue Bluish green Orange Red Purplish red Near infrared Wavelength 400 450 500 550 600 650 700 nm Fig 6 3 Wavelength and color of light When white light is irradiated on some substance and the substance absorbs the blue light it appears yellow which is the additive complementary color of blue If blue monochromatic light is irradiated on this substance the light is absorbed and the substance appears black indicating that no color exists sa Color ica y Wees Color E y 400 435 Violet Yellow green 560 580 Yellow green Violet 435 480 Blue Yellow 580 595 Yellow Blue 480 490 Greenish blue Orange 595 610 Orange Greenish blue 490 500 Bluish green Red 610 680 Red Bluish green 500 560 Green Purplish red 680
4. Fig 3 12 3 Wear gloves Hold the new WI lamp at the top and bottom so as not to taint its beam port 4 Insert the new WI lamp into the socket Push it forward until the two pins of the WI lamp contact the back of the socket and stop NOTE The two pins of the WI lamp do not have polarity Either pin can be the upper side Fig 3 13 5 Return the WI lamp retainer spring removed in procedure 1 to the original position 6 Reinstall the D2 lamp to the original position Make sure that no paper or cloth used for the work is left in SCH the light source compartment l o 7 Install the light source compartment cover by the ngs reverse procedure of that described in E Removing the light source compartment cover in this section 8 Insert the electric plug into the outlet and switch ON the UV 1800 Press the side on the switch ee UV 1800 SERIES i 3 11 3 4 Replacing the Light Source The initialization process starts Verify that the EE Initialization initialization for all items is successfully completed LSI Initialize i e OK is displayed for all items ROM Check RAM Check Filter Initialize 1 0 When the Mode menu screen is displayed follow the Laan Motor Initialize a procedure in 3 3 2 Resetting Procedure to reset WL Motor Initialize the WI lamp usage time WI Lamp Energy WL Origin search D2 Lamp Energy WL Check 2007 05 31 Fig 3 16 3 12 MH
5. 700 Purplish red Green 6 10 M UV 1800 SERIES HE 6 3 Spectrophotometer Basics 6 3 2 Ultraviolet Visible Spectrum The energy E of light can be expressed as follows E ch A c is the velocity of light h is Planck s constant and A is wavelength When light is irradiated on a substance the light of certain wavelengths is absorbed according to the molecule structure of that substance This happens as the result of the fact that the electrons existing at the ground state of the molecule absorb light energy and a transition to excitation state occurs The amount of absorption differs depending on wavelength and so the absorption spectrum the curve measuring absorption when monochromatic light is irradiated to a substance with varying wavelengths becomes unique to that substance The analysis of substances based on this principle is called absorptiometry and this method allows 1 Identification 2 Quantitative analysis and 3 Analysis of electron state Also the excited molecule loses energy due to heat and collision with other molecules and returns to its original ground state This process is called radiationless transition In addition the molecule may emit the absorbed light energy as light when returning to the ground state Fluorescent light and phosphorescence exhibit the behavior and analysis utilizing these phenomena is called fluorometry Excited state 1 Excited state 2 Light
6. A UV 1800 SERIES IN 6 25 6 5 List of Cells Quartz cell Optical width Standard required sample volume 5 0 ml or more Name Shape S cell Capacity of cell Special holder Remarks Unnecessary For general Flow cell optical path length 200 34670 reet 4x36 but front plate use Without 10 mm with holes is tube necessary Square flow cell ultra micro for syringe sipper optical path length 10 mm Q 208 92114 30 ul oi Unnecessary With tube Standard required sample volume 0 9 ml or more Square flow cell micro for syringe sipper light path length 10 mm R 208 92113 80 ul ER Unnecessary With tube Standard required sample volume 1 0 ml or more Square flow cell semi micro for syringe sipper light path length 10 mm S 208 92005 390 ul 3 5 x 11 Unnecessary With tube 6 26 MH UV 1800 SERIES HE 6 5 List of Cells N a d L WIA y 12 ESCH e A do LE Alz Se eet a SSS EISS E LEA w E A a LA XA E unit mm Fig 6 19 Optional Cell Shapes A UV 1800 SERIES IE 6 27 Cleaning the Cell Remove the sample from the cell immediately after the completion of measurement After measurement of water solution samples wash the cell with water thoroughly then wash it with ethanol lightly and let it dry well Wash with distilled water d Wash with ethanol y Dry If the cell is stained remove the sta
7. Absorbance Fluorescence Radiationless transition Phosphorescence Ground state Fig 6 4 Molecule energy A UV 1800 SERIES IE 6 11 6 3 Spectrophotometer Basics 6 3 3 Bouguer Beer s Law This law which is the basic principle of quantitative analysis is also called Lambert Beer s law Sample concentration c Incident light lo Transmission light lo x 10 Em Optical pass length Fig 6 5 Bouguer Beer s law When light with the intensity of lo is irradiated on a certain substance and the light with the intensity of has transmitted the following relational formula is established where K stands for proportional constant At this time l lo is called transmittance T l lo x 100 is percent transmittance T and 1 T log lo I is called absorbance Abs T I lo 10760 Abs log 1 T log lo l k c 1 As known from the above formula transmittance is not proportional to the concentration of the sample but absorbance is proportional to the concentration of the sample Beer s law and is proportional to light path length Bouguer s law Also the proportional constant at the time when the light path length is 1 cm and the concentration of object component is 1 mol l is called molar absorptivity and is represented by the sign of e This molar absorptivity becomes a value unique to the substance under specific conditions To fulfil Bouguer Beer s law it is necessary to satisfy condit
8. 071 60816 12 Fuse 218 004 072 02004 22 For 100 120 V 220 240 V Battery CR2032H 38 O ring 074 73306 08 036 15501 21 Used for fixing the quartz plate Alternate Sample Compartment 206 60184 07 Standard cell holder 206 82009 91 Sample compartment front cover 206 25269 ASSY Sample compartment cover 206 25269 6 8 HE UV 1800 SERIES HE Spectrophotometer Basics 6 3 1 What is Light Light is a type of electromagnetic radiation with a speed of 3 0 x 10 8 m sec in a vacuum Electromagnetic radiation includes X ray ultraviolet ray visible ray infrared ray and radio waves Wavelength 20 000 2 000 200 50 10 25 700 400 200 50 0 05 A mm um um nm nm nm nm pm Long wave Intermediate wave Short wave Ultra short wave Microwave Radio waves Far infrared Mid infrared Thermal rays Near infrared Infrared rays Visible Visible rays gt Measured range by spectrophotometer Near ultraviolet Far ultraviolet vacuum ultraviolet Ultraviolet rays X rays yray Note 1 mm 103 m 1 um 10 m 1 nm 10 m 1 pm 1012 m Fig 6 1 Types of electromagnetic waves A UV 1800 SERIES ME 6 9 6 3 Spectrophotometer Basics Wavelength is defined as the length of a single cycle and is usually indicated by a sign called lambda
9. 0 4096 0 7759 1 7059 0 2 0 0045 0 0077 0 0126 0 0257 0 0507 0 0783 0 2119 0 4762 1 3213 0 1 0 0022 0 0038 0 0063 0 0130 0 0261 0 0409 0 1188 0 3005 1 0409 0 05 0 0011 0 0019 0 0032 0 0065 0 0132 0 0209 0 0635 0 1758 0 7799 0 02 0 0004 0 0007 0 0012 0 0026 0 0053 0 0085 0 0365 0 0790 0 4770 0 01 0 0002 0 0003 0 0006 0 0013 0 0026 0 0042 0 0134 0 0413 0 3009 0 005 0 0001 0 0001 0 0003 0 0006 0 0013 0 0021 0 0067 0 0211 0 1791 0 002 0 0000 0 0000 0 0001 0 0003 0 0005 0 0008 0 0027 0 0085 0 0791 0 001 0 0000 0 0000 0 0000 0 0001 0 0002 0 0004 0 0013 0 0043 0 0413 0 0001 0 0000 0 0000 0 0000 0 0000 0 0000 0 0000 0 0001 0 0004 0 0043 Test and Engineering Vol 9 No 9 702 1981 6 16 HN UV 1800 SERIES HE 6 3 Spectrophotometer Basics A halogen lamp or deuterium lamp are used as the light source of a spectrophotometer Because these lamps emit a continuous spectrum taking out 500 nm monochromatic light from the monochromator does not mean that only the light of 500 nm is taken out but the light in a wavelength range having a certain width is taken out Band width Area Energy of light 1 2 Band width me Wavelength width to be taken out 1 1 Total amount of energy of light 1 1 2 1 4 1 4 1 16 1 4 1 16 Fig 6 8 Band width and energy of light Because the absorption coefficient of a substance differs depending on wavelength even if the cen
10. 1800 Manufacturer SHIMADZU CORPORATION ANALYTICAL amp MEASURING INSTRUMENTS DIVISION Address 1 NISHINOKYO KUWABARACHO NAKAGYO KU KYOTO 604 8511 JAPAN Authorized Representative in EU Shimadzu Europa GmbH Address Albert Hahn Strasse 6 10 47269 Duisburg F R Germany X ME UV 1800 SERIES HE CONTENTS Chapter 1 General LI UV 1800 Configuration 1 2 V2 XCOMPONCIIS as beer a tod eet e ae do Ee 1 3 1 2 1 UV 1800 Main Body Front and Top Views oooococccnnccccnnnccccconnnccnnnnnncnnnnnnennnonnnnnnnnnnnnnnnnnnnnn 1 3 1 2 2 UV 1800 Main Body Left Side View 1 4 1 23 UV 1800 Main Body Right Side View 1 5 1 2 4 Sample Compartment e 1 6 12 e EE 1 7 1 2 6 Eight Sotirce Compartmente see aa dd le doo e ende e do o lcd de 1 8 Chapter 2 Installation 2 1 Installation EE 2 2 2 1 1 Installation Requirements and PreparatiON oooconocccinncccccononcccnonnnccnnnnnncnnnnnnnnnnnnnnnnnnnnnnnnnannns 2 2 ENEE Ee 2 3 22 CONA INO POWEN EE 2 4 2 2 1 Verifying Power Supply Voltage ccccccccceeeeeceeeeeeeeeeeeeeeeeeceaeeeeeeaeeeesenaeeeessneeeeeseneeeeeees 2 4 2 2 2 Connecting to the Power Outlet 2 5 2232 GlOUMGING D 2 6 2 3 Checking the Light Source Lamp D2 ccc cceceeeceeeeeeceeeeeeeeeeeeeeeeeeesaeeeeeeceeeeesseaeeeeeseeeeeeseeeeeeees 2 7 2 4 elen Ne EE 2 8 2 5 Turning ON the Power and Initialization oooooooocinnnncccnnnncccnnnncccnnnnnccnnnnnocnnonnnnnnnnnnnrcnnnnnrnnnnnnnn conan 2 9
11. 2 9 1 Power ON OFF a a a enti A hea A deta ee 2 9 2 5 2 Initialization Operation ccccceccceeeeeceeeeeceeeeeceeeecaeeeeecaaeeesecaeeeesenaeeeeseeeeeseseeeeteeseeess 2 10 2 5 3 Switching System Language ococcccoccccnncocccnnnnncnnnnnnoconnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnns 2 12 2 6 Instrument Baseline Correction ccccccceeeecccceeeeceeeeeeeeeeeeeaeeeeeeaeeeeecaeeeeeeaeeeeseeeeeeseseneeseneeeeeees 2 13 2 7 Performance Check after Installation c cceceeccceeeeeceeeeeeeeeeeeeeeeeeeecaeeeeesaeeeeeeeeeeeeseceeeesesneeeeeees 2 15 2 7 1 Parameter Settings 2 ccccccccceeeecceeeee cence eeeeeeeeeeseaeeesaneeeeeaeeeeeeeaeeeeesaaeeeesecsaeeeeseeeeesseeeeees 2 15 2 7 2 Performing Valda O t Eed Ae ed a 2 16 Chapter 3 Maintenance amp Inspection 3 1 Inspection and Maintenance 3 2 3 1 1 List of Periodic Inspection 8 Maintenance Hemes 3 2 3 2 Inspecting the Sample Compartment oooocccccnnoccccnnnoccnnnnnncnnnnnnnnnnnnnnnnnnnnnn cnn nnnnn nn nnnnn rn nnnnrnnnnnnncnninnnns 3 3 3 3 Checking and Resetting the Lamp Usage Time 3 4 3 3 1 Checking Procedure snicsencsirieine i ie aE aE e a 3 4 3 3 2 Resetting Procedures neah rr 3 5 3 4 Replacing the Light Source ccccceceeeccceeeecceeeeeceeeeeeaeeeeeeeseeeedeneeeeeseaeeeeeeaaeeeeseaeeseeseaeeeeseneeeetenees 3 7 3 4 1 Light Source Specifications Ae 3 7 3 4 2 Lamp Replacement Procedure cccccceessseccceeeeteeeeeseceneeeee
12. 6 6 HE UV 1800 SERIES WR 6 1 Specifications Common Function 1 Selection function of the setting conditions after power is supplied Standby at the condition setting menu of each measurement mode Specifying a condition file is possible 2 Selection function of data display digits below decimal point Abs 3 digits T 1 digit or Abs 4 digits T 2 digits 3 Number of files that can be stored built in memory Measurement conditions Max 24 files Curve data Max 8 files Table data Max 8 files 4 Accumulation time set function for fixed wavelength measurement 5 External control function UV 1800 can be controlled from PC This function is applied for the control with bundled UVProve software USB cable that supports USB 1 1 separately required ee UV 1800 SERIES ME 6 7 Maintenance Parts 6 2 1 Consumable Parts Part Name Part No Remarks Replacement Cycle Tungsten odine lamp 062 65005 Light source 2 years Deuterium lamp 062 65055 05 Light source 2 years Replacement cycle above is a recommended value 6 2 2 Maintenance Parts Part Name Part No Remarks Replacement Cycle Mirror R 30 60 FR 206 25347 91 Light source 3 years switching mirror Quartz plate 206 25346 91 3 years Lens 206 25348 91 3 years Replacement cycle above is a recommended value 6 2 3 Repair Parts Part Name Part No Remarks AC power cable
13. Connect the printer to the UV 1800 and start the validation by the following procedure Press the Settings key in the Parameter Input screen for the instrument validation Validation Semi Auto items 2 Auto items Photo accuracy Noise level Photo repeatability Stray light Base line stability Resolution Resolution D2 WL accuracy WL repeatability D2 Sample module Standard cell Input item No To start START Fig 2 14 2 In the Settings screen set 1 Auto Print and Print Init status to ON Settings 1 Auto Print ON 2 Using Multicell No 3 Press the RETURN key to return to the Parameter 3 Print init status ON Input screen for the instrument validation Input item No Fig 2 15 4 In the screen press the STARTISTOP key to start the validation Validation Semi Auto items Photo accuracy eer Photo repeatabilitull 5 The check result is printed out for each validation Stray light Base line stability item when the validation is complete Resolution Resolution D2 WL accuracy 6 Check the performance of each item by referring to ple WEE the printed result Sample module Standard cell Input item No To start START Fig 2 16 2 16 MH UV 1800 SERIES HE Chapter 3 Maintenance amp Inspection 3 1 3 2 3 3 3 4 3 5 CONTENTS Inspection and Maintenance een 3 2 Inspecting the Sample Compartment oocccconnoccccnonococononocononnnnnnnnnnnnnnnnn
14. UV 1800 SERIES HE 3 4 Replacing the Light Source E Replacing the D2 lamp 1 Wear gloves Hold the resin part of the D2 lamp and slowly pull it straight up 2 Slowly extract the D2 lamp upward to remove it from the socket Fig 3 17 3 Insert the new D2 lamp into the socket Fit the locating lug at the bottom of the D2 lamp to the socket notch Make sure that the lamp is inserted all the way 4 Install the light source compartment cover by the reverse procedure of that described in E Removing the light source compartment cover in this section Notch on the socket Locating lug Fig 3 18 5 Insert the electric plug into the outlet and switch ON the UV 1800 Press the side on the switch 1 ol Fig 3 19 A UV 1800 SERIES IE 3 13 3 4 Replacing the Light Source 6 The initialization process starts Verify that the hats tas Initialization initialization for all items is successfully completed LSI Initialize i e OK is displayed for all items ROM Check RAM Check Filter Initialize 7 When the Mode menu screen is displayed follow the Lamp Motor Initialize procedure in 3 3 2 Resetting Procedure to reset WL Motor Initialize a the D2 lamp usage time WI Lamp Energy WL Origin search D2 Lamp Energy WL Check 2007 05 31 Fig 3 20 3 14 MH UV 1800 SERIES HE Cleaning the Exterior When the instrument case sample compartment cover and operation keypad get
15. identical products in the case of products such as PCs and their peripherals and parts which have a short lifespan in the market 3 Warranty Exceptions The failures caused by the following events are excluded from the warranty even if they occur during the warranty period 1 2 The product is handled in an improper way Repairs or modifications are performed by companies or people other than our company and our designated companies This product was used in combination with hardware or software other than those designated by our company Device failures and damage to data and software including the basic software that are caused by computer viruses Device failures and damage to data and software including the basic software that are caused by power failures including power outages and sudden drops of voltage Device failures and damage to data and software including the basic software that are caused by powering off the device without the proper shutdown procedure Failures caused by reasons other than the device itself Failures caused by use in harsh environments such as in high temperature or humidity corrosive gas or vibration Failures caused by fires and earthquakes or any other act of providence contamination by radio active substances and hazardous substances or any other force majeure event including wars riots and crimes Problems occur because the device is transferred or transported after instal
16. monitor display keyboard and printer USB interface etc After the sample side beam and reference side beam are picked up by detectors photodiodes and converted into the voltage by the pre amplifier the signal is then fed into an A D converter and finally read by the CPU On energy measurement mode of spectrum mode only the signal from the sample side beam is read In this case S R switching status is Normal If the status Reverse only the signal from the reference side beam is read WI Reference Pre Cell PD Amp O gt A D Converter HE O gt A D Converter Sample Cell D2 Filter Wavelength Control Control Lamp Control CPU LightSource Switch Control USB I F Personal Computer Fig 6 18 Schematic of electrical system 6 24 MH UV 1800 SERIES HE List of Cells Table 6 1 List of Optional Cells and required sample volume of 100 ul more Name shape Quartz S cell Glass G cell Q ty Special holder Square cell optical length 10 mm A 200 3442 200 34565 1 Not required Square cell matching type A 201 98716 200 98746 2 sets Not required Sealed type square cell optical length 10 mm B 200 34444 200 34444 01 1 Not required Semi microcell optical length 10 mm required c 200 66501 200 66501 01 4 Not required sample volume 1 0mL or more Semi micro black cell optical len
17. necessary to reduce the influence of fluorescent light as much as possible by increasing the distance between the sample and the detector or by inserting a mask between the sample and the detector Stray light is the total of the light of the wavelength deviated from a certain spectrum width having the set wavelength of the monochromator placed in the center and the light emitted from monochromator but does not transmit the sample and pass the side of the sample For example with 0 1 of stray light included in the monochromatic light when a sample with the wavelength of o and absorbance of 2 transmittance 1 is measured because 0 1 stray light is added in addition to 1 of the light with the wavelength of A o the transmitted light will have 1 1 of transmittance absorbance 1 959 causing 2 error As known from this the higher the sample absorbance is the larger the error due to stray light becomes To reduce stray light it is generally effective to form a double monochromator by connecting two monochromators Although it is expensive due to the complex mechanism the amount of stray light is reduced from one fiftieth 1 50 to one one thousandth 1 1000 of that of normal single monochromator Table 6 1 Absorbance error due to stray light Stray Absorbance light 0 8 1 0 1 2 1 5 1 8 2 0 2 5 3 0 4 0 1 0 0222 0 0370 0 0595 0 1150 0 2081 0 2967 0 6150 1 0370 2 0000 0 5 0 0133 0 0190 0 0309 0 0615 0 1169 0 1739
18. the items shown in Fig 2 5 The time required for this initialization is approximately four minutes nitialization ag Light Source Lamp Icon nitia l P at d e n Indicates the illumination status of LSI Initialize OK the light source lamp W and D stand for the halogen ROM he ck 0K lamp and deuterium lamp RAM Check OK respectively This icon is displayed a hen the 1 is ON and not Filter Initialize OK displayed when itis OFF A bi displayed when it is OFF A blinking Lamp Motor Initialize icon indicates that the lamp is OFF zt WL Motor Initialize OK EE WI Lamp Energy OK WL Origin search OK D2 Lamp Energy OK WL Check OK 2007 05 31 System Ver 1 00 Fig 2 5 Initialization screen 2 10 HE UV 1800 SERIES HE 2 5 Turning ON the Power and Initialization Table 2 1 Initialization items Initialization items Description LSI Initialize Initializes each I O device ROM Check Checks program ROM RAM Check Checks memory elements RAM Filter Initialize Detects the reference position of the stray light filter Lamp Motor Initialize Detects the reference position of the motor that drives the light source switching mirror WL Motor Initialize Detects the mechanical wavelength origin position WI Lamp Energy Checks whether or not the WI halogen lamp light energy is at a sufficient level WL Origin search Checks 0 order l
19. usage time data through this function as a reference for lamp replacement write down the usage time on the inspection record periodically For the service life of each lamp refer to 3 4 1 Light Source Specifications 3 3 1 Checking Procedure Press the CF3 Mainte key in the Mode menu screen 2 The Maintenance screen Fig 3 2 appears and the accumulated usage time of the light source lamps are displayed 3 Press the RETURN key to return to the Mode menu screen Fig 3 1 Mode menu H 0 0002 1 Photometric 2 Spectrum 3 Quantitation 4 Kinetics fue Scan 6 Mult i Component 7 Bio Met hod 8 Utilities Input item No Maintenance 1 Validation 2 Instrument Baseline Correction Corrected date 05 12 28 15 12 10 3 Reset lamp usage time WI lamp usage time 32hours D2 lamp usage time 62hours KeCalibration 5 Security settings WI Lamp life 2000hrs P N 062 65005 D2 Lamp life 2000hrs P N 062 65059 05 Input item No Fig 3 2 3 4 EE UV 1800 SERIES WR 3 3 Checking and Resetting the Lamp Usage Time 3 3 2 Resetting Procedure After replacing the light source lamp reset the data of accumulated usage time by the following procedure For the light source replacement refer to 3 4 2 Lamp Replacement Procedure The procedure for resetting the lamp usage time is given below using the D2 lamp as an example Press the CF3 Maint key in the Mode menu screen Fig 3 3 Mode menu 1 Ph
20. 0 UV 1800 SERIES 6 19 6 3 Spectrophotometer Basics E Single Monochromator and Double Monochromator A single monochromator system has one monochromator and a double monochromator system has two monochromators aligned in a series Accordingly when two monochromators are aligned in parallel as in the case of a two wavelength spectrophotometer the system is not called a double monochromator even though it has two monochromators The double monochromator disperses the monochromatic light emerging from the first monochromator again by means of the second monochromator So stray light is greatly reduced and a calibration curve of good linearity is obtained even though absorbance becomes high allowing analysis of samples with a broad concentration range WI Halogen lamp D2 Deuterium lamp W Window plate S1 3 Slit M1 12 Mirror G1 2 Grating F Filter CH Chopper mirror PM Photomultiplier Fig 6 14 Double monochromator configuration UV 2500 series 6 20 M UV 1800 SERIES HE 6 3 Spectrophotometer Basics de M4 M15 ve Y InGaAs PbS M14 SE Ref e W4 W5 SO M12 yA wou A smp PMT D2 Deuterium lamp S1 Entrance Slit S2 Intermediate slit S3 Exit Slit F Filter G1 G2 Diffraction grating First monochromator G3 G4 Diffraction grating Second monochromator C H Chopper mirror M2 g pe si wi d Y HQ S3 f E M1 Reference M
21. 2 deuterium lamp Fig 3 8 Light source appearances A UV 1800 SERIES i 3 7 3 4 Replacing the Light Source 3 4 2 Lamp Replacement Procedure WARNING e Before replacing the lamp turn OFF the instrument power switch and remove the electric plug from the outlet Failure to do so may result in fire electric shock and instrument malfunction DO NOT turn ON the instrument power while the light source compartment is visually exposed Ultraviolet light may be generated a serious health hazard e Before replacing the lamp turn OFF the instrument and let it stand until the lamp cools down sufficiently Touching the lamp when it is still hot will burn you CAUTION e When removing and installing the light source compartment cover avoid hitting the protrusion on the top of the D2 deuterium lamp Fig 3 8 against the back of the cover Doing so may cause a vacuum leak in the lamp tube e Be sure to wear gloves when handling the light source so as not to leave fingerprints on the glass part A fingerprint will burn onto the bulb when the light source gets hot and light transmission will deteriorate e Be careful not to break the lamp e When replacing the WI halogen lamp your hand may contact the D2 lamp Cover the D2 lamp with a clean paper or cloth or remove the D2 lamp before the work e After inserting the WI lamp into the socket DO NOT forcedly move it to right and left or up and
22. 3 4 Replacing the replacement Light Source D2 deuterium lamp O 3 4 Replacing the replacement Light Source 3 2 MH UV 1800 SERIES HE Inspecting the Sample Compartment CAUTION DO NOT spill water or organic solvent on the UV 1800 Doing so may result in electrical failure or instrument malfunction When analyzing liquid sample check that no solution sample is spilled on the sample compartment before and after measurement Should you spill any liquid sample wipe it up immediately NOTE If spilled sample is left in the sample compartment for a while it evaporates and fills the compartment corroding the internal components and interfering with correct measurement results If solution sample is spilled on the sample compartment bottom wipe up the spilled solution after removing the sample compartment unit For the procedure to remove install the sample compartment unit refer to 4 2 Removing Installing the Sample Compartment Unit Standard ee UV 1800 SERIES i 3 3 Checking and Resetting the Lamp Usage Time The UV 1800 has a function to record and display the accumulated usage time of the WI halogen and D2 deuterium lamps used for the light source Even though the accumulated usage time is saved to the instrument after the power is turned OFF the data will be cleared if any electrical problem occurs Therefore if you wish to use the
23. 7 2 Ju Beam N M9 P w2w3 me S CH Sample Beam M8 WI Halogen lamp M1 15 Mirror PbS PbS cell InGaAs InGaAs cell PM Photomultiplier Reference Reference side cell Sample Sample side cell W1 W3 Window plate 30 mm W4 W5 Window plate 40 mm Fig 6 15 Double monochromator configuration UV 3600 series A UV 1800 SERIES IE 6 21 Measurement System 6 4 1 Optical System A schematic of the optical system for the UV 1800 is shown in Fig 6 16 DAA PD a CES po CL Som D2 Deuterium lamp L M WI Halogen lamp M1 M5 F Filter B S GR Grating L S1 Entrance slit Sam S2 Exit slit Ref W Window Plate P D L M Light source switching mirror Mirrors Beam splitter Lens Sample cell Reference cell Photodiodes Fig 6 16 Schematic of optical system The light coming from the light source deuterium lamp D2 or halogen lamp WI is reflected by mirror L M and then enters the monochromator Light source switching is entirely automatic with the instrument selecting the next light source by rotating the mirror L M according to the wavelength Deuterium lamp 190 nm to light source switch wavelength Halogen lamp Light source switch wavelength to 1100 nm default setting 340 8 nm The light source switch wavelength can be set anywhere in the range from 295 0 to 364 0 nm 6 22 M UV 1800 SERIES HE 6 4 Measurement System Wit
24. 800 main body by the procedure described in 4 2 2 Installing the Sample Compartment Unit Fig 4 17 Snapping the sample compartment front cover 6 Close the sample compartment cover Fig 4 4 ee UV 1800 SERIES i 4 9 4 3 Removing Installing the Sample Compartment Front Cover This page is intentionally left blank 4 10 MH UV 1800 SERIES HE Chapter 5 Troubleshooting CONTENTS 5 1 Errors during Initialization secesii ai a E aa eE 5 2 5 2 Problems Symptoms and Golutons nennt 5 4 ee UV 1800 SERIES i 5 1 Errors during Initialization After the power is turned ON the UV 1800 initializes and checks the following items listed in the table below Initia ization Gem 151 Initialize OK a a ROM Check OK oi RAM Check OK ee ee A ter Initialize OK Zo Motor Initialize OK a Motor Initialize OK Ee eege Lamp Energy gt OK O WL Origin search OK cae Lamp Energy OK Ou WL Check OK 2007 05 31 Buste Ver 1 00 Fig 5 1 Initialization screen Table 5 1 Initialization items Item No Initialization items Description LSI Initialize Initializes each I O device O ROM Check Checks program ROM 6 RAM Check Checks memory elements RAM Filter Initialize Detects the reference position of the stray light filter 6 Lamp Motor Initialize Detects the reference position of the motor that drives the light
25. E MARE PRE MIER HTZ DATA Deeb PEF ry a 2007 05 31 System Ver 1 00 Fig 2 6 NOTE The language mode is switched only when turning ON the power switch with the ENTER key pressed When not intending to change the language mode turn ON the power switch as usual without holding down the ENTER key 2 12 MN UV 1800 SERIES HE Instrument Baseline Correction After installing the UV 1800 perform the instrument baseline correction The UV 1800 has the following two types of baseline correction Instrument Baseline Correction This corrects the optical balance of the spectrometer itself The baseline is corrected with a shorter interval within the entire wavelength range 190 nm 1100 nm and the correction data is stored NOTE If the instrument baseline correction is interrupted the stored data is not overwritten 2 Baseline Correction This corrects the baseline within the specified wavelength range to 100 T 0 Abs This correction is completed quickly since it is executed within a relatively wide interval The UV 1800 uses the correction data obtained from CU when performing Therefore the steps and shock noises can be removed in a short time Usually they can be removed only when corrections are performed with the same interval as that used when the measurement data is obtained The procedure to perform instrument baseline correc
26. INSTRUCTION MANUAL System User s Guide UV 1800 SHIMADZU SPECTROPHOTOMETER 206 97040A Feb 2008 Read the instruction manual thoroughly before you use the product Keep this instruction manual for future reference E SHIMADZU CORPORATION KYOTO JAPAN ANALYTICAL amp MEASURING INSTRUMENTS DIVISION This page is intentionally left blank Introduction Read this manual thoroughly before using the instrument Thank you for purchasing this instrument This manual describes installation operation hardware validation cautions for use and details on accessories and options Read the manual thoroughly before using the instrument Use the instrument in accordance with the manual s instructions Keep this manual for future reference IMPORTANT If the user or usage location changes be sure this Instruction Manual is always kept together with the product If this documentation or the warning labels on the instrument become lost or damaged promptly obtain replacements from your Shimadzu representative To ensure safe operation read the Safety Instructions before using the instrument To ensure safe operation contact your Shimadzu representative if product installation adjustment or re installation after the product is moved is required COPYRIGHT Information in this publication is subject to change without notice and does not represent a commitment on the part of the vendor Any errors o
27. N OA O O SSI AI RRA ARALAR AAN EII EOI 200 250 300 350 400 450 nm Water Methanol Ethanol Isopropanol Methylcyclohexan Cyclohexan Acetnitrile Hexane Dioxane Glycerin n pentane Dichloromethane Chloroform Metylacetate Metyl formic acid Carbon tetrachloride Dimethyl sulfoxide Benzene Xylene Toluene Ethylene tetrachloride Acetone Bromoform Nitro methane Carbon disulfide 450 nm Fig 6 7 Wavelength range for solvent to be used using a 10 mm cell ee UV 1800 SERIES IE 6 15 6 3 Spectrophotometer Basics 6 3 7 Calibration Curve Curvature A calibration curve is generally straight However it may bend due to various reasons The probable causes are 1 drift of measurement circuit 2 fluorescent sample stray light broad band width and measurement at the spectrum shoulder Asa spectrophotometer has some drift immediately after power is supplied to it it should be allowed to warm up for 30 min to one hour Also drift may be observed over a long period of time so it is important to check the wavelength accuracy and measurement accuracy periodically using a filter etc When a sample emits fluorescent light and if that light enters the detector the absorbance may appear low and the amount of fluorescent light increases as sample concentration becomes higher As a result the calibration curve may be bent toward the lower side If this occurs it is
28. Touching the lamp when it is still hot will burn you CAUTION e When removing and installing the light source compartment cover avoid hitting the protrusion on the top of the D2 deuterium lamp Fig 3 8 against the back of the cover Doing so may cause a vacuum leak in the lamp tube e Be sure to wear gloves when handling the light source so as not to leave fingerprints on the glass part A fingerprint will burn onto the bulb when the light source gets hot and light transmission will deteriorate e Be careful not to break the lamp According to the procedure described below check to be sure that the light source D2 deuterium lamp did not become dislodged from the designated position during transportation For details on the components on the light source compartment and the procedure to remove the cover refer to 3 4 Replacing the Light Source 1 Remove the light source compartment cover i 2 Check to be sure that the D2 lamp is seated well in the socket with no gap D2 lamp EA Socket If the lamp is mounted at an angle or there is any gap reinstall the lamp so that there is no gap in TEE EPP ege between i 3 Reinstall the light source compartment cover I I x Mounted at an angle X There isa gap Fig 2 3 Checking D2 lamp installation UV 1800 SERIES i 2 7 Operation Precautions E Precautions Before Operation CAUTION e Before turning ON t
29. aitenance aptent tordo 6 8 6 2 1 Consumable Parts ssri a a ae T A T aT ai eia 6 8 6 2 2 Maintenance Parts EE 6 8 6 2 3 Repair Hate mine e oiei ii ii iii 6 8 6 3 Spectrophotometer Basics 6 9 631 E ae Lu 6 9 6 3 2 Ultraviolet Visible Spectrum ccccccceeeececeeeeceeeeeeeeeeeeeeeeeeeeaeeeeeeeeeeeeeseeeeeeesneeeeseenseeetees 6 11 63 3 Bouguer Beers Law iere A ee Ee EES Ee 6 12 6 3 4 Qualitative Analysis and Quantitative Analyse 6 13 63 9 lt Calibration Curve a eege EES EE 6 14 6 3 67 Solvent Selection a ee a Eet Set 6 15 6 3 7 Calibration Curve Curvature cccccccceeeeneeeeeeeceeeeeeeeeeeecaeeeeeecaeeeeseeaeeeeeeaeeeeseeeeeesneeeeeees 6 16 6 3 8 Spectrophotometer Tvpes nn nnnnnnnnnnnnnnnnnnnnenininnes 6 18 6 4 Measurement Gvstem nc R RR RR nr rr nnnn nr rra nina 6 22 6 4 1 Optical Systemics ine a 6 22 0 4 2 Electrical Syste Mirrie e ted ett eE e ta eaei ei arearen 6 24 6 5 Listof Gelle EHNEN EEN AEN 6 25 6 6 Cleaning the TE 6 28 XI RE UV 1800 SERIES HE Chapter 1 General CONTENTS 1 1 UV 1800 Configuration ici 1 2 1 2 ee CEET oc beret ot a ol 1 3 ee UV 1800 SERIES IE 1 1 UV 1800 Configuration This instrument is shipped with the following items Upon opening the shipping container confirm that all of the listed parts are accounted for in your shipment Fig 1 1 Standard Contents Table 1 1 Standard Contents
30. and reference beam alternately enter one detector So unlike the double detector configuration the result is less likely to be influenced by the difference in characteristics of the two detectors WI Halogen lamp D2 Deuterium lamp W Window plate S1 2 Slit M1 10 Mirror G Grating F Filter CH Chopper mirror PM Photomultiplier Fig 6 12 Double beam single detector configuration UV 2400 series Double beam double detector configuration In this configuration the sample beam and the reference beam enter different detectors respectively Thus it is necessary to use two detectors with similar characteristics The advantage of this configuration is that it is not necessary to always pass two beams to the same detector as in the case of the single detector configuration and so a larger space is possible in the sample compartment convenient for measuring unclear samples by keeping them in close contact with the light receiving surface However in the case of the photomultiplier this configuration is not used because matching the detector is difficult WI Halogen lamp D2 Deuterium lamp W Window plate 1 2 Slit L M Light source switching mirror M1 5 Mirror GR Grating F Filter B S Beam splitter Sam Sample side cell n Ref Reference side cell A U SR L Lens PD L Ref P D Photodiode E i PD D Sam Fig 6 13 Double beam double detector configuration UV 180
31. and the UV 1800 main body fit together snugly If they don t press the sample compartment unit down to the sample compartment bottom 2 Tighten two of the sample compartment fixing screws Fig 4 4 to fix the sample compartment unit NOTE Align the screw holes on the sample compartment unit with the knurled screws by moving the unit back and forth 3 Close the sample compartment cover Fig 4 4 4 6 MH UV 1800 SERIES HE Removing Installing the Sample Compartment Front Cover To install some special accessories such as syringe sipper P N 206 23890 91 it is necessary to install the designated front plate on the sample compartment In such a case remove install the sample compartment front cover by the procedure described below For installing removing those special accessories refer to the instruction manual of each special accessory Fig 4 11 Front plate for syringe sipper switching unit 4 3 1 Removing the Sample Compartment Front Cover and Installing the Front Plate 1 Remove the sample compartment unit from the UV 1800 by the procedure described in 4 2 1 Removing the Sample Compartment Unit Turn the sample compartment unit upside down Press the tabs 2 places on the cover in the arrow direction as shown in Fig 4 12 to release them from the unit Fig 4 13 Sample compartment unit without cover Ts Sample compartment front cover Fig 4 13 Samp
32. ation 5 Security settings WI Lamp life 2000hrs P N 062 65005 D2 Lamp life 2000hrs P N 062 65055 05 Input item No Fig 3 7 3 6 EE UV 1800 SERIES HE Replacing the Light Source 3 4 1 Light Source Specifications The UV 1800 uses two types of light source lamps D2 deuterium lamp and WI halogen lamp The D2 lamp and WI lamp are used for ultraviolet region 190 nm light source switching wavelength and visible near infrared region light source switching wavelength 1100 nm respectively The closer the lamp service life comes to its end the smaller the light intensity of each lamp and the greater the noise in photometric data Replace the light source lamp by referring to the Rating life described in the table below 1 The light source switching wavelength can be arbitrarily specified within the range between 295 nm and 364 nm in increments of 0 1 nm For details refer to the Operation Guide 14 1 Setting Instrument Parameters 4 Light source 2 The rating life below has been determined based on average life of a large number of lamps by the lamp manufacturer Please note that some lamps may burn out before they reach the end of their rating lives Table 3 1 Name Part No Type Rating Life WI halogen lamp 062 65005 NA55917 Appox 2000 hours D2 deuterium lamp 062 65055 05 L6380 Appox 2000 hours Protrusion on top A E Beam port Beam port WI halogen lamp D
33. cifications Operating the UV 1800 at supply voltages other than the specified could cause fire electric shock and instrument malfunction Power voltage indication Fig 2 2 The following table shows the power supply voltage power consumption and frequency of the UV 1800 Power Supply Voltage Indication on product nameplate Power Consumption EISqueney 100 V 120 VAC 220 V 240 VAC 100 120 220 240 V AONA iS Verify that the outlet to be used has a sufficient power capacity Insufficient power capacity may cause blackouts and voltage drops affecting other instruments that use the same power supply The range of the allowable voltage fluctuation is within 10 If the fluctuation exceeds 10 be sure to use a voltage stabilizer 2 4 MH UV 1800 SERIES HE 2 2 Connecting Power 2 2 2 Connecting to the Power Outlet WARNING Handle the power supply cable carefully Follow the warnings below to avoid cable damage and the consequent risk of fire electric shock and instrument malfunction Do not place heavy objects on the power supply cable Keep thermal appliances away from the power supply cable Do not modify the power supply cable Do not forcefully bend or stretch the power supply cable Hold the plug when inserting in and removing the cable from the power outlet Should the power sup
34. d Use the power supply that satisfies power satisfy the the instrument power supply power supply specification of the UV 1800 P2 4 specification of the UV 1800 NG is displayed next Perform an appropriate action by to initialization items following the instruction in 5 1 Errors P5 2 during Initialization Nothing appears on Is the display contrast Adjust the display contrast by using the screen properly adjusted the keys while Cr Ca key Weg holding down the LCDCONT key on the keypad Numbers cannot be Is an incorrect value Enter the correct value entered from the being entered keypad Example GOTO WL 1150 nm Photometry values are Is the light source Check if the light source lamp icon odd lamp lit W or D is displayed Tag Dm 0 897548 1S KxAbs Fig 5 4 E When the icon is not displayed Only frame is displayed P2 9 P3 7 Action The lamp is currently OFF 1 Turn OFF the UV 1800 power switch and turn it ON again 2 When the result of the energy check during initialization is NG US Fig 4 1 replace the lamp by the procedure in 3 4 Replacing the Light Source 5 4 WE UV 1800 SERIES HE 5 2 Problems Symptoms and Solutions applies Correction Symptom Typical Cause Remedial Action meterenge Page Photometry values are Is the light source E When the icon is blinking odd lamp lit Action The lamp is currently OFF with some erro
35. d in this manual is as follows LZ Indication of location for related information in the instruction manuals 1 WE UV 1800 SERIES HE Installation Site Precautions WARNING When using flammable and toxic samples be sure to install ventilation equipment at an installation site CAUTION e The weight of this instrument is 15 kg During installation consider the entire weight combined with other instruments The lab table on which this instrument is installed should be strong enough to support the total weight of this instrument It should be level stable and have depth of at least 600 mm Otherwise the instrument could tip over or fall off the table e Avoid installation sites that are exposed to corrosive gases or excessive dust These adverse conditions may be detrimental to maintaining instrument performance and may shorten its service life A UV 1800 SERIES MA iii Installation Precautions To ensure safe operation contact your Shimadzu representative for installation adjustment or re installation after moving the instrument to a different site WARNING Take measures to prevent the instrument from falling in the event of earthquake or other disaster Strong vibrations could cause the instrument to fall over resulting in injury e The power supply voltages and power consumptions of this instrument are listed below The power supply
36. dirty or stained wipe them with a dry soft cloth or tissue Remove more stubborn stains by the following procedure 1 Dip a cloth into watered down mild detergent and wring it well Wipe the instrument with it Dip a cloth into water and wring it well Wipe off any detergent residue on the instrument completely Then wipe the moisture off with a dry cloth NOTE DO NOT leave any spilled water on the UV 1800 DO NOT use alcohol or paint thinner solvents for cleaning Doing so may cause the instrument surface to rust or discolor A UV 1300 SERIES i 3 15 3 5 Cleaning the Exterior This page is intentionally left blank 3 16 HN UV 1800 SERIES HE Chapter 4 Replacing the Sample Compartment Parts CONTENTS 4 1 Removing Installing the Cell Holder 4 2 4 2 Removing Installing the Sample Compartment Unit Standard cceceeceeeeeeeeeeeeeeeeeeeeeneeeetees 4 4 A3 Removing Installing the Sample Compartment Front Cover 4 7 ee UV 1800 SERIES IE 4 1 Removing Installing the Cell Holder To install some special accessories such as Ultra micro cell holder P N 206 14334 it is necessary to replace the standard cell holder in the sample compartment In such a case remove install the cell holder by the following procedure Standard cell holder Fig 4 1 Sample compartment standard Sample compartment cover Knurled screw 4 1 1 Removing the Cell H
37. down The connection part between the pin and glass may crack which makes it impossible for the lamp to illuminate 3 8 EE UV 1800 SERIES 3 4 Replacing the Light Source E Removing the light source compartment cover Using a Philips screwdriver loosen the fixing screw located on the side of the light source compartment cover Fixing screw Lift up the side part of the cover to release the notch a a ae from the fixing screw Notch on the light source compartment cover Fixing screw 3 While sliding the light source compartment cover and lifting it up at an angled following the arrow direction in Fig 3 9 remove it from the main body Fig 3 9 Removing the light source compartment cover ee UV 1800 SERIES i 3 9 3 4 Replacing the Light Source WI lamp D2 lamp 2 WI lamp socket 6 Light source switching mechanism WI lamp retainer spring Fig 3 10 Interior of light source compartment E Replacing the WI halogen lamp When replacing the WI halogen lamp your hand may contact the D2 lamp Cover the D2 lamp with a clean paper or cloth or remove the D2 lamp before the work To remove the D2 lamp refer to E Replacing the D2 lamp in this section 1 Remove the WI lamp retainer spring from the top of the WI lamp 3 10 MH UV 1800 SERIES HE 3 4 Replacing the Light Source 2 Pull out the WI lamp from the socket
38. e ultraviolet near infrared area it is measured The latter is broadly included in colorimetric analysis A UV 1300 SERIES i 6 13 6 3 Spectrophotometer Basics 6 3 5 Calibration Curve The quantitative method to measure the concentration of a sample with unknown concentration from the absorption of a sample with known concentration is provided in two methods 1 Calibration curve method and 2 Standard additive method Calibration curve method 2 Standard additive method Absorbance of unknown Absorbance Concentration of unknown sample Absorbance Concentration of unknown sample Standard sample concentration Standard sample concentration Fig 6 6 Calibration curve In the calibration curve method standard samples are operated according to an established method and then measured for absorbance A calibration curve is prepared by using the absorbance obtained here in the vertical axis and the standard sample in the horizontal axis There are times when the calibration curve does not make a straight line such as when the solution to be measured is a suspension Although the calibration curve is sure to pass the origin when a blank solution is used if it is not used the curve may not pass the origin Next the concentration of the object components in the unknown sample is obtained using this calibration curve In the standard additive method standard sample is added by stages to four or more samp
39. e instrument They could ignite and cause a fire CAUTION e Do not use mobile phones near the instrument They could damage data Precautions for Instrument Inspection Maintenance Adjustment and Care WARNING e Unplug the instrument before inspection maintenance or parts replacement Otherwise electrical shock or short circuit accidents could occur Never remove the main cover This may cause injury or malfunction of the instrument The main cover does not need to be removed for routine maintenance inspection and adjustment Have your Shimadzu representative perform any repairs requiring removal of the main cover e Ifthe power cord plug gets dusty remove the plug from the power outlet and wipe away the dust with a dry cloth If dust is allowed to accumulate fire could result e Replacement parts must be of the specifications given in 1 1 UV 1800 Configuration and 6 2 Maintenance Parts Use of any other parts may result in instrument damage and malfunction e If any water gets onto the instrument wipe it away immediately to prevent rust Never use alcohol or thinner solvents for cleaning the instrument They could cause discoloration e Dispose waste liquid properly and in accordance with the instructions of your administrative department ee UV 1300 SERIES MS v Emergency Operation In an emergency situation press the O side of the power switch l
40. eeeeeseeeeeseeeeeeeees 2 9 Instrument Baseline Correction cccscccccceeeeeeesseeeeeeececssecsaeeeeeeeeseeseeeeeeeeessceeeeeeeeeeeesseeniaeeeeeseess 2 13 Performance Check after Installation ooonnnnncococccccccnononononnnnnnnonononnnnnnnnncnnnnnnn nn nnnnncnnnannonnnnno 2 15 Installation Site 2 1 1 Installation Requirements and Preparation To use the UV 1800 properly and safely install the instrument in an appropriate location that meets the following requirements WARNING When using flammable and toxic samples be sure to install ventilation equipment at installation site CAUTION DO NOT install the UV 1800 in an environment filled with dust or corrosive gas These conditions will adversely affect the durability and performance of the UV 1800 e DO NOT install the UV 1800 near an instrument that produces strong magnetic fields Magnetic fields may adversely affect the accurate performance of the instrument Filters may be added to the power supply lines to reduce any electrical noise e To ensure instrument performance the installation site should meet the following requirements e The ambient temperature must be between 15 C and 35 C with minimal temperature variations Air currents from air conditioners and heating systems must be avoided Exposure to direct sunlight must be avoided The site must be free from vibration Humidity must rema
41. eeseaeeeaeeeeeeessaseaeaeeeeeeensneeaaaees 3 8 30 Gleaning the Exterior ee 0 tobe Ak ca de a ee a O o a ad Cel 3 15 Chapter 4 Replacing the Sample Compartment Parts 4 1 Removing Installing the Cell Holder 4 2 4 1 1 Removing the Cell Holder 4 2 4 1 2 Installing the Cell Holder 4 3 4 2 Removing Installing the Sample Compartment Unit Standard cceecceeeeeeeeeeeeeeeeeeeeeeeeeeeees 4 4 4 2 1 Removing the Sample Compartment Unit ccccecceeceeeeeeceeeeeeeeeeeeeeeeeesaeeeeesseeeseeneeeees 4 4 4 2 2 Installing the Sample Compartment Unit ccccccceeeecceeeeeeceeeeeeeeeeeeeeeeeeeesaeeeeesseeeeeesneeeees 4 5 A3 Removing Installing the Sample Compartment Front Cover 4 7 4 3 1 Removing the Sample Compartment Front Cover and Installing the Front Plate 4 7 4 3 2 Installing the Sample Compartment Front Cover 4 8 ee UV 1800 SERIES IE xi Chapter 5 Troubleshooting 5 1 Errors during Initialization coocccccncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnninininnn 5 2 5 2 Problems Symptoms and Solutions cccccccceeeecceeeeeeceeeeeeeceeeeceeeeseecaeeeseecaeeeseeeeeeeeseneeeesesneeeetees 5 4 Chapter 6 Reference CN NEE e EE 6 2 6 1 1 Hardware Specifications ccccccceesccceesecceeeeeeeeeeeeeeeeeeeceaeeeeeeaeeeeeeaaeeeeseaeeeesseeeeesseeeesenees 6 2 6 1 2 Software Gpecfcations nn nnnnnn nn nn naar n nn narran 6 3 6 2 M
42. es 1 6 HE UV 1800 SERIES HE 1 2 Components 1 2 5 Keypad e conr a pe Fig 1 6 Keypad No Name Description STARTISTOP This is the key for starting and stopping measurement once the parameter setting has been completed e When you press this key the absorbance transmittance at the current wavelength will automatically be set to OAbs 100 T Make sure that prior to sample measurement a blank cuvette is placed in both sample and reference sides UTO ZER o o ges This is the key that is used to change the current wavelength O ENTER When you enter a value press this key after the value to set the entering value Use these keys to move the cursor on the screen upward downward or left right The left cursor key can also be used to enter a negative value when entering numeric values i 2 These are the keys corresponding to the functions that are displayed at the bottom of the screen Gels EE RETURN Use this key to display to the previous screen 9 a Ss S Use this key to adjust the screen contrast Using cursor keys Ca Cv while holding down this key changes the contrast O PRINT Use this key to output a hard copy of the screen C Use these keys to enter numeric values 40 Use this key to clear a numeric value entry error When you press this key the numeric value which has been entered wi
43. gth 10 mm D 200 66551 4 Not required required sample volume 1 0 mL or more Ultra micro cell Ultra micro black cell optical path length 5 mm K 208 92116 4 holder 206 Required sample volume 25 ul or more 55050 91 is req d 3 Super micro EE rere tet nade 206 q P H 55050 91 req d A t y Super micro E M 200 66578 12 1 cell holder 206 q P H 55050 91 req d L Optical length 200 34448 200 34448 01 E 1 10 mm E quartz window glass window EE L 20 200 34472 200 34472 01 4 de Cylindrical cell ZU mm quartz window glass window Cylindrical cell OD 25p holder 204 z quartz window glass window 200 34473 02 200 34473 04 L 100 mm 1 quartz window glass window L 20mm 200 34446 200 34446 01 1 Long path S rectangular cell Square long absorption cell L 50mm G 200 34944 200 34944 01 1 holder 204 L 100 mm 200 34676 200 34676 01 1 23118 01 req d L 1mm 200 34660 01 200 34662 01 1 Short optical Short optical length cell L 2mm H 200 34655 200 34662 11 1 length cell L 5mm 200 34449 200 34449 01 EES for 1mm 204 21473 03 aoe for short optical length Tse 2 mm J 204 21473 01 1 Not required for 5mm 204 21473 02 Micro multi cell Micro multi cell 8 cells optical length 10 mm and holder req d required sample volume of 100 ul more N 208 920989 1 206 23680 91 206 23690 91 Micro multi cell Micro multi cell 16 cells optical length 10 mm p 208 92088 4 holder req d 206 23680 91 206 23690 91
44. h the exception of the light sources and light source mirrors the optical system is constructed so as to prevent exposure to dust and contaminants The monochromator slit aperture is fixed at 1 nm The Shimadzu blazed holographic grading is used for the diffraction grading The light coming out of the monochromator passes through a stray light cutting filter F and strikes the mirror M3 and is then split by the beam splitter B S into the sample side beam and the reference side beam which then pass through their respective cells and strike the detectors photodiodes The relationship between the positions of the cell holders and the beams is as shown in Fig 6 17 The image of the exit slit S2 appears near the cell position in the sample compartment The dimensions of the cross section of the beam on the image plane are Width approx 0 8 mm Height approx 9 mm Whenever possible please use black cells for the microcells when using them as they are minimally affected by scattered light CS 6 5 List of Cells Cell Holder Light beam uw Fig 6 17 Positional relationship of cell holder cell and light beam ee UV 1300 SERIES ME 6 23 6 4 Measurement System 6 4 2 Electrical System A schematic of the electrical system for the UV 1800 is shown in Fig 6 18 The center of control is the microcomputer CPU which performs all controls of light sources switching of light sources filter switching wavelength scanning pulse motor LCD
45. he power switch check to be sure that nothing is placed in the sample compartment and cell holder If the power is turned ON when a sample cell is mounted to the UV 1800 the light source energy check and wavelength check may be judged as NG since the light beam is obstructed When this occurs first turn OFF the power switch remove the cell and then turn ON the power switch again e If Sipper 160 special accessory is mounted turn ON the power switch with the flow cell filled with distilled water If the sample remains halfway within the cell the light source energy check and wavelength check may be judged as NG since the beam is refracted or scattered on the remaining sample If this occurs turn OFF the power switch and turn it ON again while pressing down the sipper 160 suction lever After the pump of the sipper 160 starts rotating suction the distilled water from the sample suction port When the distilled water starts being drained release the lever and finish the suction operation E Precautions During Operation CAUTION e Keep the sample compartment cover closed during measurement or 100 T 0 Abs correction Any outside light detected on the spectrometer may interfere with accurate measurement and correction NOTE 100 T 0 Abs is the function to correct the current photometric value to 100 T for transmittance measurement and 0 Abs for absorbance measurement Performing this correctio
46. ial Action Reference Page Neither light source lamp lights Is the Cooling fan working Check if air is expelled from the exhaust port located on the left side rear of the instrument If the fan has stopped turn OFF the UV 1800 power switch and contact your Shimadzu representative It failed in the access to the file Cannot load this file Is the USB memory or its file system intact Use a normal USB memory Did you unplug the USB memory during file access Reconnect the USB memory and retry accessing the file Is the USB memory write protected Turn OFF the USB memory write protection function Is the USB memory drive encrypted by its security function Has the file been saved in the UVProbe Cancel the encryption of the USB memory The files once saved in the UVProbe cannot be loaded on the UV 1800 Has the file been properly saved The selected file may be corrupted In that case the file cannot be loaded 5 6 MH UV 1800 SERIES HE Chapter 6 Reference CONTENTS 6 1 Ee e e EE 6 2 6 2 Maintenance Parts arnes dr trade 6 8 6 3 Spectrophotometer Basics cc ccceceeseeceeeeeeeeeeeeeeceeeeeeaeeceeaeeeeseaeeeeeseaeeceeescaeeeeseeeeeeeeeeeeenieeeeteaees 6 9 6 4 Measurement Gvstem nr rnnnnnnnnnnn nr RRRn RR Rnn nn DN NR NRRnnnnnnnnnn nn rrnnnnnnnnnnnnnrrnnnninns 6 22 6 5 Be EE 6 25 6 6 Cleaning the Cells tee e ach
47. ight which is the optical origin D2 Lamp Energy Checks whether or not the D2 lamp deuterium lamp light energy is at a sufficient level WL Check Checks wavelength by detecting the emission line at 656 1 nm using the D2 lamp Each item is initialized in order and if the initialization of the item is properly completed OK is displayed next to the item If any abnormality is detected however NG is displayed and the initialization process is terminated In such a case note the item for which NG is displayed and turn OFF the instrument To learn the check points when an error occurred refer to 5 1 Errors during Initialization ee UV 1800 SERIES IE 2 11 2 5 Turning ON the Power and Initialization 2 5 3 Switching System Language You can arbitrarily change the UV 1800 system language If Japanese language instruction manuals are necessary please contact your Shimadzu representative and purchase them separately e UV 1800series System Use s Guide e UV 1800series Operation Guide A P N 206 97039 sio dic cia P N 206 97041 E Switching Procedure English to Japanese 4 Turn ON the UV 1800 by pressing the I side of the power switch Fig 2 4 while holding down the 1 ol ENTER key on the keypad 2 The UV 1800 starts up in Japanese mode and the initialization operation begins IS 2 5 Turning ON the Power and Initialization LS 1 JHA E ROMA xy 4 RMF 4 24345 BRE HR
48. ill does not illuminate replace the WI lamp by the procedure described in 3 4 Replacing the Light Source O Is there anything Turn OFF the UV 1800 power switch Remove P2 9 obstructing the beam the obstruction and turn ON the power switch in the sample again compartment cell holder Is the light source If the icon is not displayed i e only frame is P2 9 lamp icon D displayed it means that the WI lamp is OFF P3 7 displayed on the upper right of the screen Initialization a LSI Initialize OK ROM Check gt OK RAM Check OK Fig 5 3 Turn OFF the UV 1800 power switch and turn it ON again If the lamp still does not illuminate replace the WI lamp by the procedure described in 3 4 Replacing the Light Source ee UV 1800 SERIES i 5 3 Problems Symptoms and Solutions Check whether the problem exhibits the following symptoms before requesting a repair Contact your Shimadzu representative if the error cannot be resolved through the remedial action described below or if the symptom is not covered in the table Ref Symptom Typical Cause Remedial Action eee Page Turning ON the power Is the AC power cable Connect the AC power cable plug switch does not supply plug connected correctly P2 5 the power properly Is the AC power cable Replace the AC power cable with the Wa P1 2 trapped underneath or cable of the same type if it is P2 5 twisted damaged Does the supplie
49. in between 35 and 80 No condensation Humidity must be maintained at under 70 at ambient temperatures over 30 C 2 2 MI UV 1800 SERIES HE 2 1 Installation Site 2 1 2 Installation Space CAUTION e The weight of the UV 1800 is 15 kg When selecting the installation location take into account the total weight of the UV 1800 and other devices Use a desk or stand for installation that can sufficiently support the total instrument weight having a flat and stable surface with at least 600 mm depth If these requirements are not satisfied the instrument may tip over or fall down causing an accident e Ensure that there is at least 100 mm of clearance between the left side of the UV 1800 and the wall The power supply unit and the light source unit cooling fan are located on the left side of the instrument If location specifications are not met the air cooling system with fan may work improperly overheating the instrument and deteriorating its performance The dimensions of the UV 1800 are as given in the figure below 490 mm 270 mm Fig 2 1 UV 1800 SERIES i 2 3 Connecting Power 2 2 1 Verifying Power Supply Voltage WARNING The power supply voltage is indicated on the power supply connector on the side of the instrument Be sure to connect the power supply that meets the indicated spe
50. in by dipping the cell into cleaning agent or acid Dip into cleaning agent 30 C 50 C Approx 10 minutes y Wash with distilled water y Dip into diluted nitric acid small amount of hydrogen peroxide Approx 30 minutes y Wash with distilled water y Dry If the cell gets stained with organic matter first dip it into an organic solvent such as acetone and then wash it with distilled water However when washing the flow cell for the sipper 160 series special accessory it is required to replace the PVC tube for peristaltic pump since the tube corrodes and hardens from the solvent Be sure to fill the flow cell with distilled water after washing If the flow cell is left empty the cell may get stained and it becomes hard to clean afterwards 6 28 MH UV 1800 SERIES HE Record of Revision Date Revision Changed Page Description 2 2008 A Front 2 15 6 2 6 8 and more A Record of Revision Note A Added Page No D Deleted Page No
51. ions such as being free from stray light emission scattering and reflection 6 12 MH UV 1800 SERIES HE 6 3 Spectrophotometer Basics 6 3 4 Qualitative Analysis and Quantitative Analysis To analyze what a substance is and what substances it consists of is called qualitative analysis while analysis of the amount of these substances is called quantitative analysis E Spectrum and Chemical Structure Qualitative Analysis The absorbance of ultraviolet visible light is determined by chromophore functional group that absorbs light such as C C C O N N and N O having multiplet bonding and auxochrome functional group that bonds with chromophore and changes its absorbance position and intensity such as OH NH2 and SH having non bonding electron pair and so is related to the chemical structure In this case absorbance may change depending on introduction of substitution group and types of solvent Movement of the absorbance wavelength to a longer wavelength is called bathochromic movement and its movement to a shorter wavelength is called hypsochromic movement Also an increase of absorbance is called the hyperchromic effect and a decrease is called the hypochromic effect E Colorimetric Analysis Quantitative Analysis Analysis to perform quantitative analysis by comparing the color darkness of a substance is called colorimetric analysis When the substance is transparent if absorbance exists in the invisibl
52. lamp If the deuterium D2 lamp should be broken or its life is finished dispose of the lamp separately from general garbage When disposing of the deuterium D2 lamp supplied from Shimadzu Corporation select a method which will not adversely influence the environment or human body or ask a special disposal dealer for advice or assistance The materials of deuterium D2 lamp are as follows e Metals Tungsten e Quartz glass e Ceramic e Plastic Action for Environment WEEE To all users of Shimadzu equipment in the European Union Equipment marked with this symbol indicates that it was sold on or after 13th August 2005 which means it should not be disposed of with general household waste Note that our equipment is for industrial professional use only Contact Shimadzu service representative when the equipment has reached the end of its life They will advise you regarding the equipment take back a y y g g quip WEEE Mark With your co operation we are aiming to reduce contamination from waste electronic and electrical equipment and preserve natural resource through reuse and recycling Do not hesitate to ask Shimadzu service representative if you require further information A UV 1800 SERIES IE ix Regulatory Information For Europe The product complies with the requirements of the EMC Directive 2004 108 EC and Low Voltage Directive 2006 95 EC Product Name UV Visible Spectrophotometer Model Name UV
53. lation Expendable items and parts Note Recording media such as floppy disks and CD ROMs are considered expendables If there is a document such as a warranty attached to the product or there is a separate contract agreed upon that includes warranty conditions the rules stated in those documents shall be followed Warranty periods for products with special specifications and systems are provided separately viii MN UV 1800 SERIES HE After Sales Service and Replacement Parts Availability After Sales Service If any problem occurs with this instrument inspect it and take appropriate corrective action as described in the Section Troubleshooting If the problem persists or symptoms not covered in the Troubleshooting section occur contact your Shimadzu representative Replacement Parts Replacement parts for this instrument will be available for a period of seven 7 Availability years after the discontinuation of the product Thereafter such parts may cease to available Note however that the availability of parts not manufactured by Shimadzu shall be determined by the relevant manufacturers Disposal Precautions E Disposal of UV 1800 When disposing of the UV 1800 contact your Shimadzu representative Otherwise be sure to dispose of the product separately from general garbage in compliance with the applicable laws or regulations in the country or region where it is used E When disposing of deuterium D2
54. le compartment unit UV 1800 SERIES 4 7 4 3 Removing Installing the Sample Compartment Front Cover 3 Install the sample compartment unit without cover to the UV 1800 main body by the procedure described in 4 2 2 Installing the Sample Compartment Unit 4 Install the designated front plate special accessory to the sample compartment unit Fig 4 14 Installing the front plate 5 Close the sample compartment cover Fig 4 4 4 3 2 Installing the Sample Compartment Front Cover 1 Open the sample compartment cover and remove Sample compartment front plate the designated front plate special accessory 2 Remove the sample compartment unit from the UV 1800 main body by the procedure described in 4 2 1 Removing the Sample Compartment Unit Fig 4 15 Sample compartment front plate 4 8 EE UV 1800 SERIES HE 4 3 Removing Installing the Sample Compartment Front Cover 3 Fit the protruded parts on the sample compartment unit to the corresponding protruded parts on the front cover Protruded part on the front 2 places cover lt Protruded part on the Z sample compartment unit d mp Fig 4 16 Fitting the sample compartment front cover 4 Snap the sample compartment front cover into the sample compartment unit Push the cover in the arrow direction in Fig 4 17 until it snaps 5 Install the sample compartment unit to the UV 1
55. les of measurement sample solution of the same concentration Similarly to the calibration curve method a relation curve between added value and absorbance is prepared The concentration of the object component in the unknown sample is obtained from the point where the related curve crosses the vertical axis This method is applied only when the related curve is straight as far as to the low concentration range Generally extra large absorbance wavelength is used as measurement wavelength for quantitative analysis 6 14 MH UV 1800 SERIES HE 6 3 Spectrophotometer Basics 6 3 6 Solvent Selection Generally when a sample is analyzed it is measured as a solution Accordingly the type and the concentration of the solvent must be adequate A solvent that dissolves the sample well and that is free from mutual action has small absorbance in measurement wavelength range and has small volatility is desired A cell with a lid is necessary for volatile solvent As a solvent water is excellent for measuring absorbance in visible ultraviolet range as it has no absorbance itself On the other hand many of the normally used organic solvents are transparent to the human eye so it can be mistakenly believed that absorbance does not exist in ultra violet range either The solvents and their available operating wavelength ranges are as follows 200 250 300 350 400 EELER Sy ARAN LANA ARANA RARA AO Y RR SSES ESSES SSS SSS SEELE
56. ll be cleared and then you may reenter the appropriate value A UV 1800 SERIES IE 1 7 1 2 Components 1 2 6 Light Source Compartment O Fig 1 7 Light Source Compartment No Name Description O Deuterium Lamp D2 This is the ultraviolet range 190 nm to light source switch wavelength Lamp light source For the D2 lamp replacement procedure refer to 3 4 Replacing the Light Source O Tungsten Halogen This is the visible amp near infrared range light source switch wavelength Lamp WI Lamp to 1100 nm light source For the WI lamp replacement procedure refer to 3 4 Replacing the Light Source 6 3rd light source on You can install a light source other than the standard lamps or the installation site introduction unit of external light source Light source switch wavelength The light source switch can be set anywhere in the range from 295 nm to 364 nm in 0 1 nm increments For details refer to the Operation Guide 12 2 Explanation for Setting Instrument Parameters 4 Light Source Switching Wavelength 1 3 MH UV 1800 SERIES HE Chapter 2 Installation 2 1 2 2 2 3 2 4 2 5 2 6 2 7 ee UV 1800 SERIES i 2 1 CONTENTS INTE e RE 2 2 Connecting POWST shee tena sa 2 4 Checking the Light Source Lamp D 2 7 Operation RE e EE 2 8 Turning ON the Power and Initialization cccccecccceeeecceeeeeeceeeeeeeeeeeeceaeeeeeeaeeeeeeea
57. lt Scan range 190 nm to 1100 nm ow lt Scan speeds Fast Medium Fast Medium Slow Very slow 4 Vertical axis recording range Abs 4 000 to 4 000 Min range 0 001 to 0 001 T E 400 0 to 400 0 Min range 0 1 to 0 1 5 Scan repetitions 1 to 99 6 Recording method Overlay Sequential selectable 7 Reduce Expand zoom Display data with cursor 8 Save Load data function A UV 1800 SERIES i 6 3 6 1 Specifications Quantitation 1 Measurement method One wavelength Two wavelength Three wavelength quantitation Quantitation by derivative 1 to 4 order calculation Functions regarding calibration curve Automatically calculate concentration by K factor method Automatically calculate concentration by one point calibration curve method Multi point calibration curve 1 to 3 order regression calibration curve Number of standard samples 1 to 10 Select pass through origin conditions Display of calibration curve formula Display of relative coefficient of calibration curve Measurement parameter Quantitation by repeat measurement 1 to 10 times and taking the average measurement value Save Load table data function Automatic data print function Results are automatically output on hard copy printer for every measurement Special accessories e Linked measurement with multi cell 8 16 micro multicell and CPS 240 is possible e Sipper linked measurement with the ASC 5 i
58. m of the sample Notch on the sample compartment compartment unit 2 Open the sample compartment cover to remove the sample compartment unit 1 Pull out the sample compartment in the direction that releases the notch from the fixing pin NOTE DO NOT loosen the fixing pin You can release the notch without loosening the fixing pin Fig 4 5 2 While slightly lifting up the sample compartment unit pull the unit out from the fixing pin at an angle Fig 4 6 4 4 IR UV 1800 SERIES 4 2 Removing Installing the Sample Compartment Unit Standard 4 2 2 Installing the Sample Compartment Unit CAUTION Fix the sample compartment unit securely to the instrument main body using the fixing screws knurled screws If the sample compartment unit is not seated properly outside light from the gap makes it impossible to obtain accurate measurement data 1 Open the sample compartment cover and install the sample compartment unit 1 At an angle from above insert the notch on Notch on the sample GE the sample compartment unit to the locating compartment unit pin at the far side of the sample compartment 2 Push the sample compartment unit forward so that the notch is pressed into the locating pin Fig 4 8 ee UV 1300 SERIES i 4 5 4 2 Removing Installing the Sample Compartment Unit Standard 3 Make sure that the sample compartment unit front cover
59. mechanism Monochromator Czerny Turner spectrometer Uses blazed holographic grating Detector Silicon photodiode Sample Compartment Interior dimensions 110 x 250 x 115 mm W x D x H Dimensions 450 x 490 x 270 mm W x D x H Weight 15 kg Operating Temperature 15 C 35 C Operating Humidity 30 80 No condensation 70 or less at 30 C or higher Power Supply AC 100 V 120 V 220 V 230 V 240 V 50 60 Hz Power Consumption 140 VA 6 2 MH UV 1800 SERIES HE 6 1 Specifications 6 1 2 Software Specifications Photometric One wavelength measurement 1 Photometric modes Absorbance Abs transmittance T 2 Simple quantification by K factor method 3 Save Load table data function Multi wavelength measurement 1 Photometric modes Absorbance Abs transmittance T 2 Max 8 wavelengths Wavelength setting is possible by the unit of 0 1 nm 3 Wavelength calculation function Calculation using data at up to 4 wavelengths is possible e Ratio and difference between the photometric values of two wavelengths three wavelength calculation e 4 data calculation K1 x A1 K2 x A2 K3 x A3 K4 x A4 x K5 e 4 data calculation K5 x K1 x A1 K2 x A2 K3 x A3 K4 x A4 An n 1 to 4 indicates absorbance at the measurement wavelength of An n 1 to 4 Spectrum 1 Measurement mode Absorbance Abs transmittance T and energy E N
60. mponents 1 2 3 UV 1800 Main Body Right Side View O Fig 1 4 Right Side View of UV 1800 No Name Description O USB Connector Plug in the USB memory to this connector O Power Switch Use this switch to turn ON OFF the instrument Press the I side on the switch to turn ON press the O side to turn OFF A UV 1800 SERIES IE 1 5 1 2 Components 1 2 4 Sample Compartment 7 Reference side g 1 cell holder o Measurement U 0 side cell holder a Fig 1 5 Sample Compartment No Name Description O Cell Holder The cell holder for the rectangular 10 mm light path cells has one sample cell holder and one reference cell holder LS 6 5 List of Cells Cell Holder Set Screws The cell holder can be easily removed by loosening the cell holder set screws US 4 1 Removing Installing the Cell Holder Sample Compartment Front Cover When using a flow cell etc holes are needed to pass tubing etc through Therefore this Sample Compartment can be removed and exchanged with different types of front panels US 4 3 Removing Installing the Sample Compartment Front Cover O Multi cell Holder Drive Connector This is the connector for connecting the control cables for multi cell sample compartment and 8 16 cell micro multi cell holder MMC 1600 special accessori
61. n nn cnn cnn nnnnnnnnrnnnn nn nnnnnnnnnannnnnins 3 3 Checking and Resetting the Lamp Usage Time 3 4 Replacing the Light SOUrcCe cccceeeccceeeenceeeeeeeeeeeeeseaneeeceeeeeesaaeeeetsaaeeeescaeeeesecaeeessseaeeestsneeeesseaees 3 7 Cleaning the Exterior sisses i ai e i ae eea ae aa aie a e aiae 3 15 ee UV 1800 SERIES i 3 1 Inspection and Maintenance To use the UV 1800 safely be sure to perform inspection and maintenance on the instrument WARNING Unless otherwise specified be sure to turn OFF the UV 1800 and remove the electric plug from the outlet before performing inspection and maintenance Failure to do so may result in fire electric shock and instrument malfunction CAUTION e When replacing parts always use the parts specified in 1 1 UV 1800 Configuration or 6 2 Maintenance Parts Using parts other than those specified may damage the parts causing injuries or system malfunction e DO NOT remove the cover of the UV 1800 Ignoring this may cause injuries or system malfunction For internal repair of the UV 1800 contact your Shimadzu representative 3 1 1 List of Periodic Inspection amp Maintenance Items mepecton al Everyday 1 year 2 years 3 years Reference Sample compartment 3 2 Inspecting the inspection O Sample Compartment Lamp usage time check 3 3 Checking and O Resetting the Lamp Usage Time WI halogen lamp O
62. n only for the specified wavelength is called Auto zero and performing within the specified wavelength range is Baseline correction 2 8 EE UV 1800 SERIES HE Turning ON the Power and Initialization 2 5 1 Power ON OFF Power switch Fig 2 4 UV 1800 Power Switch E Turning ON the Power 1 Press I on the power switch Fig 2 4 to turn ON the power The items to be initialized appear on the screen and start being initialized or checked sequentially IS 2 5 2 Initialization Operation 3 When all initialization items are judged OK the Mode menu screen appears NOTE If the security function is ON the Login screen is displayed prior to the Mode menu screen ISS Operation Guide 1 3 Login Screen l lof Initialization LSI Initialize ROM Check RAM Check Filter Initialize Lamp Motor Initialize WL Motor Initialize WI Lamp Energy WL Origin search D2 Lamp Energy WL Check 2007 05 31 Mode menu 1 Photometric 2 Spectrum 3 Quantitation 4 Kinetics 5 Time Scan 6 Multi Component 7 Bio Method 8 Utilities Input item No LoadP ee UV 1800 SERIES i 2 9 2 5 Turning ON the Power and Initialization E Turning OFF the Power Press O on the power switch to turn OFF the power T el 2 5 2 Initialization Operation When the instrument power is turned ON the spectrometer executes various initializations and checks for
63. o La ts a tad So EE 6 28 ee UV 1800 SERIES i 6 1 Specifications 6 1 1 Hardware Specifications Measurement Wavelength Range 190 1100 nm Spectral Band Width Resolution 110 2 nm Wavelength Accuracy 0 1 nm 656 1 nm D2 0 3 nm All region Wavelength Repeatability 20 1 nm Wavelength Scanning Speeds When shifting wavelength approx 6000 nm min When scanning wavelength approx 3000 nm min 2 nm min Light Source Switching Automatic switching with wavelength range Can be set anywhere in range from 295 nm to 364 nm 0 1 nm units Stray Light 0 02 or less 220 nm Nal 0 02 or less 340 nm NaNO 1 or less 198 nm KCl Photometric System Double beam optics Photometric Range Absorbance 4 4 Abs Transmittance 0 400 Photometric Accuracy 0 002 Abs at 0 5 Abs 0 004 Abs at 1 Abs 0 006 Abs at 2 Abs Tested by using NIST930D or NIST1930 or other appropriate standard filter Photometric Repeatability 0 001 Abs or less at 0 5 Abs 0 001 Abs or less at 1 Abs 0 003 Abs or less at 2 Abs Noise Level Within 0 00005 Abs 700 nm Baseline Flatness 0 0006 Abs 190 nm 1100 nm 1 hour after the light source is ON Baseline Stability Within 0 0003 Abs h 700 nm 1 hour after the light source is ON Light Source 20 W halogen lamp Deuterium lamp Built in light source position automatic adjustment
64. ocated on the right side bottom of the UV 1800 to turn OFF the instrument Power OFF CT side is pressed in Operation at Power Outage In case of electrical failure perform the following operations 1 Press the O side of the power switch located on the right side bottom of the UV 1800 to turn OFF the instrument 2 After the power comes back on start up the UV 1800 normally by following Installation Precautions and Operation Precautions Vi WR UV 1800 SERIES HE Warning Labels For safety operation warning labels are affixed where special attention is required Should any of these labels peel off or be damaged obtain replacements from Shimadzu Corporation Right side High Temperature Light source and light source chamber are very hot When replacing the light source be sure to turn OFF the power and check that the light source is completely cooled ee UV 1800 SERIES IE vii Product Warranty Our company provides a warranty on this product as stated below Details 1 Warranty Period Please consult your Shimadzu representative for information about the extent of the warranty 2 Warranty Description If failure occurs for reasons attributable to our company during the warranty period our company will provide repairs or the replacement of parts without charge including USB dongles However we may not be able to provide
65. od BCA method Biuret method CBB method Bradford method UV absorption method ee UV 1300 SERIES i 6 5 6 1 Specifications Maintenance and Inspection 1 Instrument baseline correction function Function Tie A 2 Display Reset of lamp lighting time 3 Security setting Faculty limitation can be set for each user account 4 Unit validation function 1 Correspond to 9 items Wavelength accuracy wavelength repeatability spectral band width stray light photometric accuracy photometric repeatability baseline flatness baseline stability noise level initialization result record 2 Semi automatic test For items requiring test jigs test can be progressed in the form of dialog 3 Full automatic test Test is automatically performed from measurement to pass fail judgment result printing 4 Setting test conditions pass fail judgment criteria 5 Detailed printing of the test results 6 Printing of all test result File Management Function 1 Convert spectrum and time course curve to CSV format 2 Copy and delete files Data Processing Function Following processings can be performed on curve data such as spectrum and time course curve e Peak Valley detection both possible up to 20 pcs e The four rules of arithmetic e Derivative processing e Smoothing processing e Area calculation e Point pick processing e Print processing printing waveform data in text format
66. older 4 Open the sample compartment cover 2 Loosen the knurled screw fixing the cell holder Remove the cell holder Cell holder 4 2 MH UV 1800 SERIES HE 4 1 Removing Installing the Cell Holder 4 1 2 Installing the Cell Holder 1 While aligning the locating hole on the cell holder with the locating pin on the sample compartment unit place the cell holder on the sample compartment unit Arrow mark Knurled screw NOTE Install the cell holder so that the beam passes through it as directed by the arrow mark 2 Fix the cell holder with the knurled screw Locating pin ao gt _ Locating hole Fig 4 3 ee UV 1300 SERIES i 4 3 Removing Installing the Sample Compartment Unit Standard To install some special accessories such as Sipper 160 series P N 206 23790 91 etc it is necessary to replace the standard sample compartment unit in the sample compartment In such a case remove install the standard sample compartment unit by the procedure described below For installing removing those special accessories refer to the instruction manual of each special accessory Sample compartment cover Sample compartment unit fixing screws Fig 4 4 Main body front view 4 2 1 Removing the Sample Compartment Unit 1 Loosen two of the sample compartment unit fixing Fixing pin on the UV 1800 screws located at the botto
67. otometric 2 Spectrum 3 Quantitation 4 Kinetics 5 Time Scan 6 Multi Component 7 Bio Method 8 Utilities Input item No 2 Press the 3 D Reset lamp usage time key in the Maintenance screen Fig 3 4 GER 1 Validation 2 Instrument Baseline Correction Corrected date 05 12 28 15 12 10 3 Reset lamp usage time WI lamp usage time 32hours D2 lamp usage time 62hours 4 2 Recalibration 5 Security settings WI Lamp life 2000hrs P N 062 65005 D2 Lamp life 2000hrs P N 062 65055 05 Input item No Fig 3 4 3 The window for selecting the lamp whose usage is to be reset appears Use the Ca Cv keys to move the cursor to D2 lamp Fig 3 5 and press the ENTER key Reset lamp usage time Fig 3 5 A UV 1300 SERIES i 3 5 3 3 Checking and Resetting the Lamp Usage Time Use the C Cr keys to select Yes and press the ENTER key 1 Validation 2 Instrument Baseline Correction Corrected date 05 12 28 15 12 10 The lamp usage time will be reset Y se WI Lamp life 2000hrs P N 062 65005 D2 Lamp life 2000hrs P N 062 65055 05 Input item No Fig 3 6 5 You will return to the Maintenance screen Fig 3 7 Verify that D2 lamp usage time is reset to 0 hours 1 Validation 2 Instrument Baseline Correction Corrected date 05 12 28 15 12 10 6 Press the RETURN key to return to the Mode menu 3 Reset lamp usage tine screen Fig 3 3 WI lamp usage time 32hours 4 4 Recalibr
68. ouble detector configuration E Single and Double Beam Configurations e Single beam configuration This configuration allows only one beam to pass through the sample compartment First set the transmittance to 100 or the absorbance to 0 using the cell filled with solvent Then replace it with the cell containing sample and perform measurement Light source Monochromator Detector Amplifier Display Sample compartment Fig 6 10 Single beam configuration Double beam configuration This configuration divides the monochromatic light into two beams using mirrors such as a rotating mirror and a semi transparent mirror so as to make two beams the sample beam and reference beam When the sample cell with sample in it is placed for the sample beam and the reference cell with solvent in it is placed for reference beam in the sample compartment each transmitted light enters the detector The feature of this configuration then is that transmittance and absorbance can be measured once from the sample sign and the reference sign lo simultaneously Reference side Light source Monochromator Detector Amplifier Display Sample side Rotating mirror semi Sample compartment transparent mirror Fig 6 11 Double beam configuration 6 18 MH UV 1800 SERIES HE 6 3 Spectrophotometer Basics E Single and Double Detector Configuration Double beam single detector configuration In this configuration a sample beam
69. ply cable become damaged contact your Shimadzu representative CAUTION Verify that the power switch of the UV 1800 is OFF Le O is pressed in before connecting the electric plug to the outlet 4 Insert the connector of the power supply cable accessory into the power supply connector Fig 1 3 on the left side of the UV 1800 2 Insert the electric plug of the power supply cable into the power outlet A UV 1800 SERIES IS 2 5 2 2 Connecting Power 2 2 3 Grounding WARNING Ground the instrument to prevent electric shock and provide reliable performance of the UV 1800 The power supply cable supplied with the UV 1800 consists of three wires including a ground wire When installing the instrument be sure to insert the cable into the three wire system outlet 2 6 EE UV 1800 SERIES HE Checking the Light Source Lamp D2 WARNING e Before opening the light source compartment cover turn OFF the instrument power switch and remove the electric plug from the outlet Failure to do so may result in fire electric shock and instrument malfunction DO NOT turn ON the instrument power while the light source compartment is visually exposed Ultraviolet light may be generated a serious health hazard If the UV 1800 has been operating before opening the cover turn OFF the instrument and let it stand until the lamp cools down sufficiently
70. r omissions which may have occurred in this publication despite the utmost care taken in its production will be corrected as soon as possible but not necessarily immediately upon detection All rights are reserved including those to reproduce this publication or parts thereof in any form without permission in writing from Shimadzu Corporation Microsoft and Windows are registered trademarks of Microsoft Corporation in the United States and or other countries Other company names and product names mentioned in this manual are trademarks or registered trademarks of their respective companies TM and marks are omitted in this manual 2007 2008 Shimadzu Corporation All rights reserved ee UV 1800 SERIES IS i Safety Instructions To ensure safe operation of the instrument read these Safety Instructions carefully before use e Observe all of the WARNINGS and CAUTIONS described in this section They are extremely important for safety In this manual warnings and cautions are indicated using the following conventions Indicates a potentially hazardous situation which if not avoided could result in serious WARNING injury or possibly death Indicates a potentially hazardous situation which if not avoided may result in minor to CAUTION moderate injury or equipment damage Emphasizes additional information that is provided to ensure the proper use of this NOTE product The symbol use
71. rs The fan is not working or the thermo censor on the circuit board has detected overheating Turn OFF the UV 1800 power switch and contact your Shimadzu representative Did you mistakenly Return to the blank condition press the reference condition and press the P1 7 key during AUTO ZERO Key again measurement Is the cell being used Do not use a glass cell in the an appropriate one ultraviolet range Is a cell phone being The measured value may be used near the influenced depending on the types of instrument cell phones and the radio waves conditions Avoid using a cell phone a near the instrument during measurement Baseline curve does When correcting the Place cells with the same solvent in not meet normal baseline did you puta both the sample side and the specifications solvent with high reference side and perform baseline IS 6 1 1 Hardware absorbance in the cell correction again Specifications holder on only one side Is the beam on only Set the beam conditions so that they one side restricted are identical on both the sample side and the reference side Are you using an Some of the unit specifications may optional accessory not be met when certain optional accessories are installed If none of the above Correct the instrument baseline three scenarios according to 2 6 Instrument Baseline P2 13 A UV 1800 SERIES IE 5 5 5 2 Problems Symptoms and Solutions Symptom Typical Cause Remed
72. s possible Kinetics Kinetics 1 2 3 Rate measurement 1 2 3 Measure Abs time change and calculate activity values Measurement time 1 to 9999 sec min Measurement using Abs difference between two wavelengths is possible Measure Abs time change and calculate the change in absorbance quautity Measurement time 1 to 9999 sec min Determine whether the measured reaction result is progressing in linear fashion 6 4 WE UV 1800 SERIES HE 6 1 Specifications Time Scan 1 Measure time course change in photometric values 2 Measurement mode A Absorbance Abs transmittance T energy E 3 Measurement time 1 to 9999 sec min 4 Linked measurement with multi cell 8 16 micro multicell and CPS 240 is possible 5 Reduce Expand zoom Display data with cursor 6 Save Load data function Multi Component Analysis 1 Up to 8 components 2 Pure and mixed samples of each constituent component can be used as standard samples 3 Besides measurement wavelengths standard sample data can be filed 4 Quantitation can be done by calling up spectra Bio method DNA quantitation 1 Calculate DNA Protein concentrations and Abs ratio DNA concentration K1xA1 K2xA2 Protein concentration K3xA2 K4xA1 2 Factor measurement wavelength can be set arbitrarily 3 Background correction can be applied Protein quantitation 1 Quantitation method Lowry meth
73. source switching mirror WL Motor Initialize Detects the mechanical wavelength origin position WI Lamp Energy Checks whether or not the WI halogen lamp light energy is at a sufficient level WL Origin search Checks 0 order light which is the optical origin O D2 Lamp Energy Checks whether or not the D2 deuterium lamp light energy is at a sufficient level WL Check Checks wavelength by detecting the emission line at 656 1 nm using the D2 lamp 5 2 WE UV 1800 SERIES HE 5 1 Errors during Initialization When NG is displayed during initialization perform the appropriate remedial actions by referring to the table below If the problem is not resolved by these actions contact your Shimadzu representative Item No Check Point Remedial Action Reference Page 0 6 Turn OFF the UV 1800 power switch and turn P2 9 it ON to initialize the instrument again Is there anything Turn OFF the UV 1800 power switch P2 9 obstructing the beam Remove the obstruction and turn ON the power in the sample switch again compartment cell holder Is the light source If the icon is not displayed i e only frame is P2 9 lamp icon W displayed it means that the WI lamp is P3 7 displayed on the upper currently OFF right of the screen Initialization R LSI Initialize gt OK ROM Check OK RAM Check gt OK Fig 5 2 Turn OFF the UV 1800 power switch and turn it ON again If the lamp st
74. the instrument validation function in order to check the performance of the UV 1800 For details on how to use the function refer to the Operation Guide 16 Instrument Validation Function 2 7 1 Parameter Settings Set validation parameters in the Parameter Input screen for each item as follows E Noise level Perform the validation using the parameters below Wavelength 700 nm Tolerance DP 0 30 mAbs or less RMS 0 05 mAbs or less E Baseline flatness Perform the validation using the parameters below Scan range 1100 200 nm Tolerance 0 6 mAbs E Wavelength accuracy D2 emission line Perform the validation using the parameters below Wavelength 656 1 nm 486 0 nm Tolerance 656 1 nm 0 10 nm 486 0 nm 0 30 nm Noise level Test 1 Inspection Yes 2 Wavelength gt 700 0 nm 3 Tolerance P P 0 30 mAbs or less RMS 0 05 n bs or less Input item No Fig 2 11 Validation parameters for noise level Baseline flatness 1 Inspection Yes 2 Scan range 1100 200 nm 3 Tolerance 0 6 mAbs Input item No Fig 2 12 Validation parameters for baseline flatness WL accuracy D2 1 Inspection Yes See 656 1nm 486 0nn 3 Tolerance 656 1 0 10 nm 4 Tolerance 486 0 0 30 nm D Input item No Fig 2 13 Validation parameters for wavelength accuracy D2 emission line A UV 1800 SERIES IS 2 15 2 7 Performance Check after Installation 2 7 2 Performing Validation
75. tion is given below It takes approximately 17 minutes to complete the correction Other UV 1800 operations are not available during that period 4 Verify that nothing is set on the cell holder in the sample compartment and close the sample compartment cover 2 GE Si CF3 Maime Key MINS Mode Mend Mode menu screen Fig 2 7 Photometric 2 Spectrum 3 Quantitation 4 Kinetics fie Scan 6 Multi Component 7 Bio Method 8 Utilities Input item No L 3 Select C2 Instrument Baseline Correction in e the Maintenance screen Fig 2 8 E 2 Instrument Baseline Correction Corrected date 05 12 28 15 12 10 3 Reset lamp usage time WI lamp usage time 32hours D2 lamp usage time 62hours 4 4 Recalibration 5 Security settings Input item No Fig 2 8 ee UV 1800 SERIES i 2 13 2 6 Instrument Baseline Correction 4 Select Yes using the Cr keys and press the ENTER key Correct Instrument Baseline The instrument baseline correction is started 5 During the correction process a message will be displayed at the bottome of the screen To terminate the instrument baseline correction press the STARTISTOP key Baseline Correcting about 17 min Press STOP to stop Fig 2 10 2 14 MH UV 1800 SERIES HE Performance Check after Installation Once the installation is complete validate the performance for the following items e Noise level e Baseline flatness e Wavelength accuracy Use
76. tral wavelength is the same with different bandwidth the wavelength width of the light to be taken out varies This causes the absorption coefficient to be changed in appearance and this results in absorbance change It is sufficient to set the bandwidth of a spectrophotometer from one eighth 1 8 to one tenth 1 10 of the half width of a sample s absorbance spectrum The half width of the absorbance spectrum means the width at the half of the peak of the absorbance spectrum Because the absorbance spectrum often has a broad half width in colorimetric analysis a bandwidth of 10 nm is sufficient Making the bandwidth extremely narrow generates large noise due to energy shortage and may result in poor measurement accuracy If the bandwidth is made large the peak height becomes low in appearance and this may cause the calibration curve to be bent Peak height actually measured Real peak height 04 RS WE JI KE EEN DA 06 08 10 2 Spectrum band width Real spectrum half width Fig 6 9 Band width and peak height A UV 1800 SERIES IE 6 17 6 3 Spectrophotometer Basics 6 3 8 Spectrophotometer Types Spectrophotometers can be categorized broadly by optical systems as follows Single beam configuration Measurement optical system Double beam configuration Single detector configuration D
77. voltage of the instrument is indicated on the label on the side of the instrument Connect the instrument only to a power supply of the voltage indicated otherwise fire or electric shock could result Check that the power supply voltage is stable and that its current capacity is sufficient to operate all the components of the system If not the instrument will not operate at its rated performance Power Supply Voltage Power GE Indicated on the instrument Consumption q y AC100 V 120 V and AC220 V 240 V 100 120 V and 220 240 V E SE Ground the instrument Grounding is necessary to prevent electric shock in the event of an accident or electrical discharge and important for ensuring stable operation e Do not place heavy objects on the power cord and keep any hot items away The cord could be damaged resulting in fire electrical shock or malfunction If the cord becomes damaged contact your Shimadzu representative immediately e Do not modify the cord in any way Do not bend it excessively or pull on it The cord could be damaged resulting in fire electrical shock or malfunction If the cord becomes damaged contact your Shimadzu representative immediately iV RE UV 1800 SERIES HE Operation Precautions WARNING e Always wear protective gloves when handling any toxic or biologically infectious samples e Do not use flammable sprays hair sprays insecticide sprays etc near th

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