Data Sheet
Contents
1. MUM 1 Concentrated and Prediluted Rabbit Monoclonal Antibody ISO 9001813485 CERTIFIED Control Number 901 352 032415 Catalog Number CRM 352 A B PRM 352 AA Description 0 1 0 5 ml concentrated 6 0 ml prediluted Dilution 1 200 1 400 Ready to use Diluent Da Vinci Green N A Intended Use For In Vitro Diagnostic Use MUM 1 BCS is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of MUM 1 protein by immunohistochemistry IHC in formalin fixed paraffin embedded FFPE human tissues The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient s clinical history and other diagnostic tests by a qualified pathologist Summary and Explanation Multiple myeloma oncogene 1 MUM 1 is a 50 kDa protein encoded by the MUM 1 gene Studies have shown IRF4 MUM 1 is expressed in the nuclei and cytoplasm of plasma cells and a small percentage of germinal center GC B cells located in the light zone This antibody labels MUM 1 protein in centrocytes and their progeny plasma cells activated T cells and a wide spectrum of hematolymphoid neoplasms derived from these cells 1 3 Principle of Procedure Antigen detection in tissues and cells is a multi step immunohistochemical process The initial step binds the primary antibody to its specific epitope After labeli2. body Incubate for 30 minutes at RT Probe N A taal Biocare Medical 4040 Pike Lane Concord CA 94520 USA Tel 800 799 9499 l Page of 2 www biocare net OAI 352 T60 60 tests prediluted Ready to use N A Protocol Recommendations manual use Cont d Polymer Incubate for 30 minutes at RT with a secondary conjugated polymer Chromogen Incubate for 5 minutes at RT with Biocare s DAB OR Incubate for 5 7 minutes at RT with Biocare s Warp Red Counterstain Counterstain with hematoxylin Rinse with deionized water Apply Tacha s Bluing Solution for 1 minute Rinse with deionized water Technical Note This antibody has been standardized with Biocare s MACH 4 detection system It can also be used on an automated staining system and with other Biocare polymer detection kits Use TBS buffer for washing steps Protocol Recommendations ONCORE Automated Slide Staining System OAI352 is intended for use with the ONCORE Automated Slide Staining System Refer to the ONCORE Automated Slide Staining System User Manual for specific instructions on its use Protocol parameters in the ONCORE Automated Slide Stainer Protocol Editor should be programmed as follows Protocol Name MUM 1 Rb Protocol Template Description Rb HRP Template 1 Dewaxing DS Option DS2 Antigen Retrieval AR Option AR2 low pH 90 C Reagent Name Time Temp MUM 1 Rb 30 min 25 C Limitations The optimum antibody dilution and
3. by genes involved in chromosomal alterations in lymphoma and leukemia clinical value of their detection by immunocytochemistry Blood 2002 Jan 15 99 2 409 26 3 Johnson LR Nalesnik MA Swerdlow SH Impact of Epstein Barr virus in monomorphic B cell posttransplant lymphoproliferative disorders a histogenetic study Am J Surg Pathol 2006 Dec 30 12 1604 12 4 Center for Disease Control Manual Guide Safety Management NO CDC 22 Atlanta GA April 30 1976 Decontamination of Laboratory Sink Drains to Remove Azide Salts 5 Clinical and Laboratory Standards Institute CLSI Protection of Laboratory Workers from Occupationally Acquired Infections Approved Guideline Fourth Edition CLSI document M29 A4 Wayne PA 2014 aol Biocare Medical 4040 Pike Lane CE Concord CA 94520 Page 2 of 2 USA Tel 800 799 9499 www biocare net Fax 925 603 8080 ISO 9001813485 CERTIFIED Follow the antibody specific protocol recommendations according to data sheet provided If atypical results occur contact Buiocare s Technical Support at EMERGO EUROPE Molenstraat 15 2513 BH The Hague The Netherlands
4. ng the antigen with a primary antibody an enzyme labeled polymer is added to bind to the primary antibody The detection of the bound antibody is evidenced by a colorimetric reaction Source Rabbit monoclonal Species Reactivity Human and dog Clone BC5 Isotype IgG Total Protein Concentration 10 mg ml Call for lot specific IgG concentration Epitope Antigen MUM 1 protein Cellular Localization Nuclear and cytoplasmic Positive Control Tonsil Known Applications Immunohistochemistry formalin fixed paraffin embedded tissues Supplied As Buffer with protein carrier and preservative Storage and Stability Store at 2 C to 8 C Do not use after expiration date printed on vial If reagents are stored under conditions other than those specified in the package insert they must be verified by the user Diluted reagents should be used promptly any remaining reagent should be stored at 2 C to 8 C Protocol Recommendations manual use Peroxide Block Block for 5 minutes with Biocare s Peroxidazed 1 Pretreatment Solution recommended Reveal Pretreatment Protocol Heat Retrieval Method Retrieve sections under pressure using Biocare s Decloaking Chamber followed by a wash in distilled water alternatively steam tissue sections for 45 60 minutes Allow solution to cool for 10 minutes then wash in distilled water Protein Block Optional Incubate for 5 10 minutes at RT with Biocare s Background Punisher Primary Anti
5. protocols for a specific application can vary These include but are not limited to fixation heat retrieval method incubation times tissue section thickness and detection kit used Due to the superior sensitivity of these unique reagents the recommended incubation times and titers listed are not applicable to other detection systems as results may vary The data sheet recommendations and protocols are based on exclusive use of Biocare products Ultimately it is the responsibility of the investigator to determine optimal conditions The clinical interpretation of any positive or negative staining should be evaluated within the context of clinical presentation morphology and other histopathological criteria by a qualified pathologist The clinical interpretation of any positive or negative staining should be complemented by morphological studies using proper positive and negative internal and external controls as well as other diagnostic tests Quality Control Refer to CLSI Quality Standards for Design and Implementation of Immunohistochemistry Assays Approved Guideline Second edition I LA28 A2 CLSI Wayne PA USA www clsi org 2011 Precautions 1 This antibody contains less than 0 1 sodium azide Concentrations less than 0 1 are not reportable hazardous materials according to U S 29 CFR 1910 1200 OSHA Hazard communication and EC Directive 91 155 EC Sodium azide NaN used as a preservative is toxic if ingested Sodium azide may
6. react with lead and copper plumbing to form highly explosive metal azides Upon disposal flush with large volumes of water to prevent azide build up in plumbing Center for Disease Control 1976 National Institute of Occupational Safety and Health 1976 4 EMERGO EUROPE CE Molenstraat 15 2513 BH The Hague The Netherlands Fax 925 603 8080 MUM 1 Concentrated and Prediluted Rabbit Monoclonal Antibody Control Number 901 352 032415 Troubleshooting Precautions cont d 2 Specimens before and after fixation and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens If reagents or specimens come into contact with sensitive areas wash with copious amounts of water 5 3 Microbial contamination of reagents may result in an increase in nonspecific staining 4 Incubation times or temperatures other than those specified may give erroneous results The user must validate any such change 5 Do not use reagent after the expiration date printed on the vial 6 The SDS is available upon request and is located at http biocare net 1 800 542 2002 References 1 Carbone A et al Expression pattern of MUM1 IRF4 in the spectrum of pathology of Hodgkin s disease Br J Haematol 2002 May 117 2 366 72 2 Falini B Mason DY Proteins encoded
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