EDP Plus User Manual 2002
Contents
1. 12 KEYBOARD LOCK CONTROL F 9 The EDP Plus keyboard can be locked if desired This feature is for protection Locking the keyboard prevents unwanted volume or mode changes when the EDP Plus is used for the same task over an extended period To lock the keyboard press F 9 when the keyboard is active when KB is displayed Press F 9 again to unlock the keyboard When the keyboard is locked all keystrokes are ignored excep F O clear F 8 tone generator control and F 9 keyboard lock unlock The trigger operates whether the keyboard is locked or unlocked Setting Speed 5 PROGRAMMABLE SPEED Fe You can program the pickup and dispense speed of the EDP Plus pipette to accommodate almost any liquid including moderately dense or viscous liquids The default speed setting for EDP Plus pipettes is 8 This fast setting is optimized for liquids with similar physical properties to water However you may wish to set a higher speed for even faster operation or a slower speed for more dense or viscous liquids To reset the speed press F e decimal point then press a number between O and 9 then press E The display reverts to show volume after speed has been set The table below shows cycle component times for available speed settings Table 5 1 EDP Plus Programmable Speeds Speed Full Scale Full Scale Delay before Blowout Dwell Time Setting Pickup Dispense Blowout duration constant 9 1 3 sec2. 19 REINSTALLING THE LIQUID END CAUTION Always attach the replacement liquid end BEFORE inserting the encoder plug 1 Refer fo Figure 8 3 Hold the shaft retaining nut and support the liquid end upright with its end on a smooth soft surface such as a paper towel 2 Bring the liquid end and the control module together with the control module directly above the liquid end Actuator 3 Engage the actuator pin with the actuator seat on the Pin piston retainer 4 Push the liquid end toward the control module body and rotate the liquid end so the tip ejector pin on the control module fits through the matching hole on the liquid end shaft With the tip ejector pin in the proper position the control module key will be aligned with the keyway on the shaft Ja FIGURE 8 3 ATTACHING THE LIQUID END 5 Hold the liquid end and control module together and thread the shaft retaining nut onto the control module Ensure the threads do not cross and hand tighten the shaft retaining nut onto the control module NOTE 2 5 mL and 10 mL models do not use tip ejectors For these models engage the actuator pin into the piston retainer and thread the liquid end onto the control module Store the shaft retaining nut and tip ejector spring for use with other liquid ends 6 Take the replacement encoder plug Make sure the labels on the plug and liquid end match Also check the markings on the liquid end these are shown in Figure 9 1 7 Al
3. MULTIPLE DISPENSE NOMINAL VOLUME SETTINGS 1 The nominal pickup volume or previously stored value is displayed Press E to check or change the dispense volume 2 Press number keys to set or change the dispense volume While pressing keys the entry flashes until E is pressed See DISPENSE VOLUME below 3 The instrument calculates and flashes the largest number of whole aliquots which will divide into the nominal volume You can accept this aliquot number by pressing E or you can change to a smaller number by pressing number keys then E MULTI DISPENSE MULTI KB 235ul KB 4 A DISPENSE VOLUME NUMBER OF ALIQUOTS FLASHING 4 The instrument calculates and displays the volume needed to pick up all aliquots r PICKUP MULTI TOTAL PICKUP VOLUME ne 94 Ou l OPERATION 1 Press and release the trigger to pick up the displayed volume 2 Press and release the trigger again to dispense the first aliquot Continue pressing and releasing the trigger until all aliquots are dispensed 3 Press 0 to clear the instrument discarding the residual volume left in the tip After the dispense volume isn entered EDP Plus calculates the pickup volume Pickup Volume Number of Aliquots x Dispense Volume Residual Volume When the calculated volume is picked up the piston slightly overshoots and then returns to its proper pickup position to ensure accuracy of the first aliquot dispensed To ensure that t
4. volumes up fo 100 pL 2 to 4 mm volumes from 100 uL fo 1000 iL 3 to 6 mm volumes over 1000 pL 3 There is no significant adherence of liquid fo the surfaces of the tip Each tip should be rinsed once before pipetting to assure the best precision 4 When dispensing the tip is touched to the sidewall of the receiving vessel At the end of each dispense cycle the tip is moved along the wall to remove any excess sample from the outside of the tip A consistent rhythm is maintained from sample to sample when pipetting Viscous liquids such as serum or plasma can be measured accurately with a slow speed See Table 5 1 Programmable Speeds You can further improve accuracy with viscous solutions by using MULTIPLE DISPENSE mode with the number of aliquots set fo 1 and using a slow speed This method simulates reverse mode pipetting in manual pipettes In MULTIPLE DISPENSE mode the volume picked up includes an extra residual volume and the volume of the dispensed aliquot is accurate You can measure warm or cold liquids with good precision by using a consistent pipetting rhythm A consistent rhythm will help minimize any differences in heating or cooling effects within the pipette Use a new disposable tip each time for best accuracy and precision when measuring samples with temperatures greatly different from ambient and do not pre rinse As with any air displacement pipette best results will be obtained if there is no delay
5. MEASURE MODE Discard Ready Pick up remainder Blowout Ready PISTON f 2 A f POSITION UP HOME TAP or HOLD FLO to DOWN TRIGGER gt gt gt CLEAR gt gt gt PAUSE gt gt gt PAUSE gt gt gt Holding trigger delays this step Mt READ VOLUME BEEP BEEP MEASURED BEEP NOTES 1 An initial pickup volume can be set 2 Piston direction can be changed by pressing O even while the trigger is pressed 3 At full stroke or at zero the trigger must be released Then either press F 0 to clear the pipette or press the trigger again to reverse piston direction 11 MEASURE MODE F 5 Make sure the piston is at the HOME position KB shows on display and the keyboard is not locked Press F 5 to open MEASURE mode SETTINGS The display reads Opl or any previously entered initial pickup volume You can enter an initial pickup volume if you wish by pressing number keys then E OPERATION 1 Press and hold the trigger to pick up Pickup speed increases until it reaches the maximum Release the trigger and press it again to restart at the slowest rate To pick up one increment at a time tap the trigger Any initial pickup volume is picked up at the first trigger press Hold the trigger in to continue measurement When the desired volume of liquid has been picked up ensure the lower level of the liquid is flush with the tip ori
6. You can extend the delay by holding in the trigger as the sample is dispensed When EDP Plus is in any other mode open PIPETTE mode as follows a Press F 0 This action will clear the current operation and move the piston to HOME position KB will show on the display indicating the piston is HOME Check the keyboard is not locked If locked press F 9 to unlock it b Press F 1 fo open PIPETTE mode The display will show the nominal volume unless you have previously set a volume in PIPETTE mode In all modes any previously entered volume is remembered by EDP Plus as long as power is supplied continuously Holding the trigger in postpones the next step in all modes except TITRATE MEASURE and MIX Each piston stroke is signalled by a short beep when the tone generator is turned on End of stroke beeps will not be included in the following descriptions 6 QUICK REFERENCE MULTIPLE DISPENSE MODE Dispense Ready Pick up Multiple Blowout PISTON TO ne mE POSITION 7 a UP HOME REPEAT DOWN _ TRIGGER gt gt gt TRIGGER gt gt gt CLEAR gt gt gt PAUSE gt gt gt BEEP BEEP AFTER BEEP BEEP EACH ALIQUOT BEEP BEEP MULTIPLE DISPENSE MODE F 2 Make sure the piston is at the HOME position KB shows on display and the keyboard is not locked Open MULTIPLE DISPENSE mode by pressing F 2 PICKUP MULTI KB 1000ul
7. between picking up the sample and dispensing it 18 Changing Liquid Ends 8 EDP Plus liquid ends are interchangeable The liquid end nominal volume is marked in two places shown in Figure 9 1 The encoder plug is marked on its top Woie Mert on surface The liquid end can be mounted very quickly on oe the EDP Plus control module as described below E f me Before removing the liquid end bring the piston to TT HOME by pressing F 0 The letters KB should appear on the display If the keyboard is locked unlock it press F 9 Waima Mari on I Tip Gerir Shan FIGURE 8 1 TO REMOVE THE LIQUID END VOLUME MARKINGS ON LIQUID END 1 Referring to Figure 8 2a loosen the shaft retaining nut by rotating it counter clockwise 2 When the shaft retaining nut is free of the threads pull the liquid end away from the control module Figure 8 2b 3 Remove the encoder plug from the control module Figure 8 2c and store it with its matching liquid end Liquid measurements made with the EDP Plus will be accurate and precise ONLY when the correct liquid end encoder plug combination is used NOTE Before storage disassemble the liquid end and inspect for salts or residues Clean the liquid end and keep it with its encoder plug Do not touch the underside of the plug skin oils can contaminate it causing poor electical contact and performance The plug can be cleaned with isopropanol FIGURE 8 2 REMOVING THE LIQUID END
8. 1 3 sec 0 0 sec 0 13 sec 1 0 sec 8 1 3 sec 1 3 sec 0 5 sec 0 13 sec 1 0 sec 7 1 3 sec 1 3 sec 1 0 sec 0 13 sec 1 0 sec 6 2 2 sec 2 2 Sec 0 5 sec 0 22 sec 1 0 sec 5 2 2 sec 2 2 sec 1 0 sec 0 22 sec 1 0 sec 4 2 2 sec 2 2 Sec 1 5 sec 0 22 sec 1 0 sec 3 2 2 sec 2 2 Sec 2 0 sec 0 22 sec 1 0 sec 2 4 4 sec 4 4 sec 0 5 sec 0 44 sec 1 0 sec 4 4 sec 4 4 sec 1 0 sec 0 44 sec 1 0 sec 0 4 4 sec 4 4 sec 2 0 sec 0 44 sec 1 0 sec This table does not apply to trigger controlled actions in TITRATE and MEASURE modes Default speed setting for EDP Plus 10ML and M8 models is 5 Speed range is from O 6 13 Advanced Operation 6 Table 6 1 EDP Plus Advanced Functions FUNCTION KEY DESCRIPTION APPLICABLE MODE MIX F 6 Mixes by repeated up and P PIPETTE down piston motion while D DILUTE trigger is held in VOLUME F 7 PIPETTE up to 12 distinct P PIPETTE SEQUENCING volumes can be picked up and dispensed sequentially MULTIPLE DISPENSE up M MULTIPLE DISPENSE to 12 distinct volumes can be dispensed sequentially from a single pickup volume DILUTE up to 12 distinct D DILUTE volumes can be picked up sequentially and dispensed together MIX F 6 Available in PIPETTE and DILUTE modes only SETTINGS 1 Open the MIX function by pressing F 6 2 The display prompts you for a mix volume y MIX Enter this volume and pres
9. 2 Removing the Liquid End r 19 Figure 8 3 Attaching the Liquid End r 20 Figure 8 4 Display afer Initialization Sequence 20 Figure 9 1 Removing the Tip Ejector 21 Figure 10 1 Typical Piston Seal Assembly ccceececeeesteeteeetteeneeens 22 Figure 10 2 Removing the Piston Assembly from the Liquid End 23 Figure 10 3 2 5 mL Liquid End Removing the Piston Seal 23 Figure 10 4 10 mL Liquid End Assembly 24 Figure A 1 Connecting Wall Power Supply ccccesesteteeeseeneeees 28 Figure A 2 Rapid Charge Stand Accessory 28 Table 3 1 EDP Plus Basic Operation eerren 4 Table 5 1 EDP Plus Programmable Speeds ccccccceteessestteecees 13 Table 6 1 EDP Plus Advanced Functions 14 Table 12 1 EDP Plus Specifications 0ccccccccccceesseeeeeseeeeseesereenees 27 EDP Plus EDP and EDP2 are trademarks of Rainin Instrument LLC 1989 2002 Rainin Instrument LLC All rights reserved EDP Plus Pipettes are manufactured under U S Patent No 4 671 123 4 779 467 and 4 905 526 and under the following national patents Taiwan NI 23042 Australia AU 589891 France FR 2559904 Europe CH DE GB IT NL EP 0152120 and EP 0428500 Canada CA 1293709 Japan JP 1807271 Germany DE 3588071 and DE 3586289 Prices and specifications subject to change without notice Description 1 EDP Plus Electronic Pipettes use a motorized linear actuator to move
10. 250 EDP Plus M8 Pipette 8 x 250 microliter fixed liquid end Battery Pack 6100 080 BatteryPack for cordless operation Rechargeable by Wall Power Supply or Rapid Charge Stand Rapid Charge Stand 6101 049 Rapid Charge Stand for EDP Plus pipette US Japan 6101 048UX Rapid Charge Stand for EDP Plus pipette UK 6101 047EX Rapid Charge Stand for EDP Plus pipette Europe Extra Liquid Ends Each Liquid End is supplied with a matching Encoder Plug 6100 461 Micro 10 Liquid End 10 microliter 6100 066 Liquid End 25 microliter 6100 067 Liquid End 100 microliter 6100 068 Liquid End 250 microliter 6100 069 Liquid End 1000 microliter 6100 030 Liquid End 2 5 milliliter 6100 032 Liquid End 10 milliliter Extra Control Module 6100 462 Control Module only for all volumes 29 Extra Wall Power Supply 6100 063 Wall Power Supply 100 130V 50 60Hz US Japan 6100 064UX Wall Power Supply 210 260V 50 60Hz UK 6100 044EX Wall Power Supply 210 260V 50 60Hz Europe Extra Shafts 6100 414 EP 10 Shaft upper 6100 416 EP 10 Shaft lower Seal Retainer metal 6100 427 EP 25 Shaft 6100 428 EP 100 Shaft 6100 429 EP 250 Shaft 6100 430 EP 1000 Shaft 6100 224 EP 2500 Shaft 6100 250 EP 10 ML Shaft Assembly EC Declaration of Conformity according to ISO IEC Guide 22 and EN45014 Manufacturers Name Rainin Instrument Co Inc Manufacturer s Address 5400 Hollis St Emeryville CA 94608 declares that the following product P
11. end as shown in 2 3 Slip the shaft retaining nut from the tip ejector 4 Figure 10 1 and set it aside until you need it again 5 Slide the shaft retaining nut over the liquid end 6 Thread the shaft retaining nut carefully onto the control module threads and tighten the liquid end in place Do not overtighten FIGURE 9 1 REMOVING THE TIP EJECTOR CAUTION Do not press the trigger during this operation Care and Maintenance 10 EDP Plus pipettes are designed to need very little maintenance and should give years of trouble free service if treated with proper care and the operating recommendations in Section 8 are followed Also see RAININ Publication AB 15 Procedure for Evaluating Accuracy and Precision of RAININ Pipettes available online at http Awww rainin com pdf ab15 padf The following rules should be strictly observed to keep the mechanism dry and clean 1 Never allow liquid to enter the shaft where it can contact the piston or seal 2 Never pick up liquid without a disposable tip attached 3 Never invert EDP Plus or lay if on its side with liquid in the tip Always hold it upright 4 Never use solvents to clean EDP Plus Instead use a lint free wipe dampened with water to clean the instrument Keep the keyboard and encoder plug dry 21 SEALS Periodically each liquid end except the 10 uL should be tested to ensure proper sealing as follows 1 Set the nominal volume and attach a disposa
12. into the tip Use water as your sample until you are familiar with the EDP Plus pipette QUICK REFERENCE PIPETTE MODE Ready Pick up Dispense Blowout PISTON Fy n h POSITION UP HOME DOWN _ TRIGGER gt gt gt TRIGGER gt gt gt PAUSE gt gt gt PAUSE gt gt gt Holding in the trigger delays this step H YH BEEP BEEP BEEP BEEP PIPETTE MODE F 1 When power is supplied EDP Plus executes a five second initialization sequence and opens in PIPETTE mode The display will show the nominal volume SETTINGS Press number keys then the E Enter key to change the volume The display will flash on and off If you make a mistake set a new volume then press E OPERATION PICKUP DISPENSE a 475ul 475ul PICKUP VOLUME DISPENSE VOLUME 1 Press and release the trigger to pick up the displayed volume You will hear a beep at the end of the piston stroke See diagram at left 2 Press and release the trigger again to dispense the liquid See diagram at right The piston moves to HOME then rapidly executes a blowout stroke You will hear a beep at the end of the blowout Affer a one second delay the piston returns to HOME ready for the next pickup This is signalled by two beeps The one second delay is to give you time to remove the tip from the dispense vessel before the piston returns to HOME position
13. is at the HOME position KB shows on display and the keyboard is not locked Press F 4 to open DILUTE mode The display will show the default diluent volume which is 90 of nominal volume The default sample volume is 10 of nominal volume for 10 1 dilution SETTINGS 1 Set the diluent volume V1 by pressing number keys then E DILUTE 500ul Vi DILUENT VOLUME 2 Set the sample volume V2 in the same way DILUTE V2 SAMPLE VOLUME P 10 Ou l OPERATION 1 Press and release the trigger to pick up the diluent DILUTE Vi 500ul PICKUP PICK UP DILUENT VOLUME 10 2 Press and release the trigger fo pick up an air gap j Air DILUTE 3 Press and release the trigger a third time to pick PICKUP up the sample 100ul V2 DILUTE 4 A three part tone sounds indicating that diluent DISPENSE and sample are separated by the air gap ready to be dispensed Press and release the trigger to 600l dispense diluent and sample DISPENSE DILUENT AND SAMPLE TOGETHER 5 The display shows the diluent volume ready for the next cycle When using DILUTE mode with the 10mL liquid end the air gap will probably rise through the diluent and allow diluent and sample to mix in the sample reservoir To avoid this you can use VOLUME SEQUENCING in DILUTE mode Then set a smaller air gap or no gap as desired See Section 6 Advanced Operation VOLUME SEQUENCING QUICK REFERENCE
14. row key or E fo turn on instrument Depleted Battery Pack Recharge Battery Pack see Appendix B Wall power supply disconnected not using Battery Pack Reconnect cord to EDP Plus pipette Display reads Err warbling alarm Illegal volume entered will not be accepted Enter legal volume Keyboard will not accept Keyboard locked Unlock keyboard by entries KB locked on LCD If the suggested remedy does not correct the problem contact RAININ 800 543 4030 pressing F 9 If anything confusing happens during operation of the EDP Plus pipette it can sometimes be remedied by pressing F 0 unless the display is flashing indicating an entry must be completed Pressing F 0 returns the piston to HOME and activates the keyboard You can also reset the instrument by removing power or removing and replacing the encoder plug The keyboard MUST be unlocked if you remove the encoder plug The pipette will restart and reinitialize It will be in PIPETTE mode at its nominal volume after this sequence This remedy should be used only as a last resort since all stored information will be lost from the micro computer and will need to be re entered RAININ maintains service departments to correct any electro mechanical problems with the EDP Plus pipette or problems resulting from physical or chemical damage In the U S A please contact Technical Service at 800 543 4030 for more information and a
15. sequencing cycle can be started at any point Open the VOLUME SEQUENCING function by pressing F 7 SETTINGS Display flashes Opl or the volume last set Set the first pickup dispense volume V1 by pressing number keys followed by E Continue setting sequential pickup dispense volumes Press 0 E to end the sequence If you enter all twelve volumes you do not have to press O to end the sequence Sequential volumes are numbered as follows Vq_9 then Va 10 Vb 11y amp Vo 12 OPERATION 1 Press and release the trigger to pick up the current volume PICKUP SEQ 100p FIRST VOLUME PICKED UP 15 2 Press and release the trigger to dispense the current volume DISPENSE SEQ 100u 1ST VOLUME DISPENSED 3 Press and release the trigger fo pick up the next sequential volume PICKUP SEQ 150ul 2ND VOLUME PICKED UP 4 Press and release the trigger to dispense the next sequential volume Continue in this way until all volumes are picked up and dispensed 5 After the last volume in the sequence is dispensed the first volume is displayed again ready for pickup 6 To close the VOLUME SEQUENCING function press F 7 VOLUME SEQUENCING MULTIPLE DISPENSE MODE In MULTIPLE DISPENSE mode with VOLUME SEQUENCING up to 12 distinct volumes can be dispensed sequentially from one pickup volume Open the VOLUME SEQUENCING feature by pressing F 7 SETTINGS 1 The display fl
16. 00 full stroke cycles before charging is needed If the Battery Pack discharges during operation the EDP Plus will sound a warning and the message Lob will be displayed If this occurs connect the EDP Plus to a charger immediately after the current pipetting cycle If you connect to the wall power supply you can continue operation without interruption The Battery Pack can only be charged when installed in EDP Plus in either of two ways WALL POWER SUPPLY Connect the wall power supply cord fo the connector on the back of the control module Connect the power supply fo an AC power source with the correct line voltage as specified on the power supply 105 130 Volts AC 50 60 Hz or 210 260 Volts AC 50 60 Hz The wall power supply can recharge a completely discharged Battery Pack in about 14 hours RAPID CHARGE STAND Connect the Rapid Charge Stand to a suitable AC power source with the correct voltage See paragraph above Place EDP Plus on the stand so that the four contact pins on fit into the contact slots on the stand The display reads FC Fast Charge functions are inoperative until EDP Plus is removed from the stand The Rapid Charge Stand can recharge a Battery Pack in about 90 minutes Current is metered precisely and the Battery Pack temperature continually monitored via a built in sensor probe When the Battery Pack is fully charged the red charge indicator light on the Rapid Charge Stand goes out For extended sto
17. EDP P lus Electronic Pipette 9 pipettes for q volume ranges 0 5 uL to 10 mL f from 250 uL EDP Plus shown E i RAININ Contents Section 1 D SCIIPTION esievicsesevevresicsees E AE EEA Section 2 Unpacking and Set up 0 ccccccccecccccesecceeescteesseeetsessseeeseeenees Section 3 Basic Operation sesta rises i ead at ees Maer 4 Section 4 Optional SettingS cccceeceeseeeseeeteesseenseeteeenseenteeens 12 Section 5 Setting Speeds 2 00 0 cececeeeeseesseesseeeseeecseenseeesteesseeneeens 13 Section 6 Advanced Operation rerne 14 Section 7 Proper Pipetting Technique ccccccccecceecetesseetteeeteenees 18 Section 8 Changing Liquid Ends erreren 19 Section 9 Removing the Tip Ejector 21 Section 10 Care and Maintenance 21 Section 11 Troubleshooting ienien aiai deeeenes 26 Section 12 Performance Specifications s 27 Appendix A Charging the Battery Pack 0 ccccccecesccsesseeteesseenteeens 28 Appendix B Ordering Information 0 ccccccececcseneteteeneetteesseeteeens 29 FIGURES AND TABLES Figure 1 1 EDP Plus Controls and Indicators 0 00ccccceceseeeeeteeeeeees Figure 2 1 Display after Initialization Sequence 2 Figure 2 2 Installing the Battery PACK 0 ccccccecseeeseesseeteeeteeeteenteees 3 Figure 3 1 Holding the EDP Plus Pipette 4 Figure 3 2 Resuming Operation after Auto Shut Off 5 Figure 8 1 Volume Markings on Liquid End s 19 Figure 8
18. ashes Opl or the volume last set Enter the first volume V1 with the number keys and press E 2 The display flashes Opl again prompting you for the next volume Enter the second volume V2 and continue in the same way for each entry finishing each entry with E and the last entry with 0 E Sequential volumes are numbered as follows Vj_9 then Va 10y Vb 11y amp Vo 12 3 The EDP Plus pipette calculates and displays the number of cycles or the largest number of times the sum of the volumes will divide into the nominal volume of the instrument Accept the number of cycles by pressing E or set a smaller number and press E Setting a larger number will sound an error alarm OPERATION 1 The display will show the volume to be picked up press and release the trigger to pick up that volume 16 2 The next trigger press will dispense the first sequential volume The next trigger press will dispense the second sequential volume and so on 3 After all sequential volumes in the first cycle have been dispensed press the trigger to begin the second cycle Continue until all cycles are complete 4 The display will read CLEAR after the last dispense volume in the last cycle Press 0 to clear the instrument 5 To close the VOLUME SEQUENCING function press F 7 VOLUME SEQUENCING DILUTE MODE In DILUTE mode with VOLUME SEQUENCING up to twelve distinct volumes can be picked up sequentially and dispe
19. ble tip 2 Pick up the full volume of water 3 Hold the instrument upright and observe the tip for 30 fo 45 seconds There should be no leaks from the tip or droplet formation at the tip orifice If leakage does occur the seal between the tip and the shaft may not be airtight DISPOSABLE TIP SEAL Use only RAININ disposable pipette tips as recommended in Section 5 These tips mate exactly with the EDP Plus liquid end shaft to form airtight seals PISTON SEAL The seal between the piston and the inside surface of the shaft is formed by a PTFE ring and a fluoroelastomer O ring except the 10 mL liquid end The PTFE ring extends through the O ring and only the PTFE ring makes contact with the piston This is shown in Figure 10 1 The 10 mL piston seal assembly is shown later in this section The O ring compresses the PTFE ring against the piston fo maintain a proper seal even after moderate seal wear and also forms a stationary seal between the PTFE ring and the shaft Periodically you should dismantle the liquid end for oo inspection and cleaning as follows Fi Taa 1 Remove the liquid end from the control module fo Do not remove the encoder plug for this procedure 2 Inside the liquid end press the spring loaded piston retainer and rotate it slightly counter Clockwise to release the retainer catch 3 Pull the piston assembly out of the liquid end Fig 10 2 The seal should stay attached to the piston If it remains
20. er Pipetting Technique 7 Use RAININ tips EDP Plus pipettes are designed for use with RAININ manufactured disposable tips Specified performance of EDP Plus pipettes is guaranteed only when RAININ disposable tips are used as recommended in Section 8 RAININ cannot accept responsibility for damage to EDP Plus pipettes or for poor performance resulting from the use of other than RAININ disposable tips RAININ disposable tips are molded from premium grade virgin polypropylene plastic Molds are inspected and maintained frequently to assure high quality tip production Samples from each lot of tips are inspected microscopically to assure that every lot meets these high standards To order Tips for EDP Plus Pipettes please refer to the RAININ Pipetting Solutions Catalog or the current RAININ Product Update Call 800 543 4030 for a free copy of these publications You can also view or download a PDF version online http Avww rainin com lit_prodlit asp Pipetting techniques EDP Plus pipettes are designed to deliver accurate and precise measurements under the following conditions 1 The liquid pipetted is water or liquid with density viscosity and vapor pressure similar to water The temperature of the room the EDP Plus and the liquid are all in the range 21 5 C 1 C The EDP Plus pipette is kept vertical while pipetting or within 20 of vertical 2 The end of the disposable tip is immersed beneath the sample surface as follows 2 to 3 mm
21. er outlet matching the line voltage printed on the label 105 130 VAC 50 60 Hz or 210 260 VAC 50 60 Hz Then plug the cord from the power supply into the socket on the back of the control module The EDP Plus pipette will execute its initialization routine After about 5 seconds the instrument will be in PIPETTE mode and the display should be as shown in Figure 2 1 If not refer to the Troubleshooting Guide in Section 11 of this manual PICKUP KB 1000p FIGURE 2 1 DISPLAY AFTER INITIALIZATION SEQUENCE EP 1000 SHOWN Your EDP Plus pipette is now ready for use If you have purchased the Battery Pack Accessory install it into the control module as described in the following pages NOTE EDP Plus can be used without a power cord when the Battery Pack is installed However before installing the Battery Pack it is a good idea to test operation with the wall power supply to see that the pipette works correctly WARNING This equipment generates and uses radio frequency energy and if not installed and used properly i e in strict accordance with the instruction manual may cause harmful interference to radio communications It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC rules which are designed to provide reasonable protection against such interference when operated in a commercial environment Operation of this equipment in a residential area is li
22. fice no air has been included and read the volume on the display The piston direction can be reversed at any time by pressing 0 This is useful if an air bubble is picked up pressing 0 reverses the flow expelling the bubble Pressing 0 again reverses flow and continues measurement The display always shows the current volume in the tip as well as the piston direction either PICKUP or DISPENSE 2 At full or zero scale the display reads CLEAR and the trigger must be released Press the trigger again to reverse direction automatically The pipette may be cleared and the tip contents dispensed by pressing F O this is useful if the volume of liquid to be picked up exceeds the full scale volume of the instrument Optional Settings 4 TONE GENERATOR CONTROL F 8 A tone generator in the EDP Plus signals operational status or error conditions as follows Status Tone sound Tone description Initialization BEEP BEEP Two short beeps Key press BIP One very short beep End of pickup stroke BEEP One short beep End of dispense stroke BEEP One short beep lower tone Piston at HOME BEEP BEEP Two short beeps End of pickup in DILUTE mode BEEP BEEP BEEP Two short one long beep Error condition Low battery DEE DAH DEE DAH High warble Works even when tone generator is OFF Tones are automatically switched on when the instrument is powered up To switch off the tones press F 8 Pressing F 8 again turns the tone generator back on
23. he last aliquot will be accurate the fixed residual volume remains in the tip after all aliquots have been dispensed and must be discarded by pressing 0 before making the next pickup stroke QUICK REFERENCE TITRATE MODE Discard remainder Ready Pick up Titrate and Blowout Ready PISTON n en ie POSITION UP HOME TAP or HOLD F 0 to J DOWN __ TRIGGER gt gt gt TRIGGER gt gt gt CLEAR gt gt gt PAUSE gt gt gt i Ul BEEP BEEP BEEP READ VOLUME BEEP DISPENSED TITRATE MODE F 3 Make sure the piston is at the HOME position KB shows on display and the keyboard is not locked TITRATE PICKUP Open TITRATE mode by pressing F 3 1000p KB TITRATE NOMINAL VOLUME SETTINGS 1 The display shows the nominal pickup volume TITRATE To change the pickup volume enter a new initial PICKUP ickup volume with the number keys and press E pickup y p a 5 60ul INITIAL PICKUP VOLUME SET 2 To set an initial dispense volume press number keys then press E To set an initial dispense TIRATE DISPENSE volume without changing the initial pickup volume press E first Enter the volume with number keys 1 50Oul Then press E again KB INITIAL DISPENSE VOLUME SET OPERATION 1 Press and release the trigger to pick up the initial volume 2 To begin titration press and hold the trigger to dispense liqu
24. id first slowly then at an increasing rate The longer the trigger is held the faster the liquid is dispensed Release and hold in the trigger again to restart dispensing at the slowest rate To dispense one increment at a time tap the trigger 3 When the desired endpoint is reached read and record the dispensed volume from the display Then clear the instrument by pressing F 0 This will discard the remainder in the tip and return EDP Plus to its starting position If the entire volume is titrated press the 0 key when the beep sounds and the display reads CLEAR This action will execute the blowout stroke and empty the residual volume from the tip In TITRATE mode a small residual volume is left in the tip after the entire volume has been titrated The residual volume ensures that the volume titrated is accurate The residual volume is not part of the titrated volume and must be discarded by pressing F 0 QUICK REFERENCE DILUTE MODE Pick up Pick up Pick up Ready Diluent V1 Air Sample V2 Dispense Blowout Ready PISTON 3 3 s POSITION e A 2 uP 1 7 F HOME A il DOWN TRIGGER gt gt gt TRIGGER gt gt gt TRIGGER gt gt gt TRIGGER gt gt gt PAUSE gt gt gt PAUSE gt gt gt A H trager delays this step UJ L L uy L U uy DILUENT AIRGAP __ SAMPLE BEEP BEEP BEEP BEEP BEEP BEEP BEEP DILUTE MODE F 4 Make sure the piston
25. ign the encoder plug with its slot in the head of the control module avoid touching the bottom of the plug and insert the plug squarely If power is supplied to the EDP Plus and the keyboard is unlocked the initialization sequence begins when the encoder plug is in place 8 After the initialization sequence look at the display The EDP Plus pipette should be in the pipette mode with the display reading PICKUP The nominal volume of the liquid end is also displayed as shown in Figure 9 4 If your display does not show the proper nominal volume check that the correct encoder plug is installed PICKUP KB 1000ul FIGURE 8 4 A DISPLAY AFTER INITIALIZATION SEQUENCE EP 1000 SHOWN 20 Removing the Tip Ejector 9 The EDP Plus pipette liquid end except 2 5 mL and 10mL has a built in tip ejector operated by pressing the thumb button The tip ejector is a hollow spring loaded moveable shaft surrounding the liquid end and extending up through the shaft retaining nut The EDP Plus pipette is normally operated with the tip ejector in place allowing disposable tips to be ejected without hand contact However when using deep narrow reservoirs you may wish to operate the pipette without the tip ejector in place Refer to Figure 9 1 and remove the tip ejector as follows 1 Loosen the shaft retaining nut counter clockwise Pull the liquid end with tip ejector from the control module Remove the tip ejector from the liquid
26. in the liquid end invert the FIGURE 10 1 PISTON SEAL ASSEMBLY liquid end and gently tap it on the bench until the seal drops out 4 Inspect the piston seal bushing and inside of the shaft for any dirt and particulate contamination There is no need to dismantle the piston assembly any further 22 5 Remove any contamination by soaking and rinsing the piston assembly with distilled water or Vy alcohol The piston surface should have a high gloss finish If the finish is dull scratched or pitted Piston the seal may wear very rapidly and the complete Assembly piston assembly should be replaced 6 Blow the assembly dry with clean dry compressed nitrogen or dry it with a very soft lint free wipe To avoid scoring the piston do not use rough material such as a paper towel Liquid End CAUTION Never apply grease to the pipette seal or piston except 10 mL Any grease or lubricant may alter the effective diameter of the piston and cause errors in liquid measurement FIGURE 10 2 REMOVING THE PISTON ASSEMBLY FROM THE LIQUID END REASSEMBLY 1 Make sure the PTFE seal and O ring are properly positioned on the piston as shown in Figure 10 1 2 Place the piston assembly into the liquid end and compress the piston retainer against the piston spring Rotate the retainer slightly clockwise to engage the retainer catch 3 Replace the liquid end on the control module attach a disposable tip and test f
27. ip the pipette tightly during operation Operate the trigger with your index finger and the tip ejector button with your thumb Because there is no need for thumb pressure during pick up dispense and blowout operator fatigue is greatly reduced compared to manual pipettes FIGURE 3 1 HOLDING EDP PLUS AUTO SHUT OFF The EDP Plus pipette shuts itself off automatically if none of the keys or the trigger are pressed for three minutes If the display is blank and the trigger does not work while power is supplied EDP Plus is shut off Press any of the top row keys or E fo turn on the instrument F 7 8 9 lt lt Press any key in this row or E D MEAS MIX when EDP Plus is shut off 4 5 6 c P M T 0 1 2 3 E FIGURE 3 2 RESUMING OPERATION AFTER AUTO SHUT OFF EDP Plus remembers current operational settings when shut off and will resume operation at the same point and with the same settings as when last used as long as power fo the instrument has not been interrupted This feature is provided to extend the life of the Battery Pack accessory BASIC OPERATION The examples used in this manual show the display for EP 1000 EDP Plus with 1000 uL nominal volume Your instrument will operate in the same way but volumes shown will be different if your pipette has a different nominal volume Attach a disposable tip by pressing the liquid end shaft firmly
28. kely to cause interference in which case the user at his own expense will be required to take whatever measures may be required to correct the interference INSTALLING THE BATTERY PACK The Battery Pack compartment is at the rear of the control module Slide the battery compartment cover left to remove it Carefully pull the male connector clear shown below Align the male and female connectors noting the pin pin arrangement one pin space two pins Fasten the connectors together but do not force them Insert the Battery Pack into the control module as indicated on the label Replace the compartment cover If the pipette does not initialize when power is supplied but worked normally when connected to the wall power supply the Battery Pack may have discharged in transit See Appendix A Charging the Battery Pack A FEMALE BATTERY PACK CONNECTOR CONTROL MODULE MALE CONNECTOR D Connect the male connector to the female connector on the new battery pack and rotate the battery pack into the control module as shown by the arrow D G x Push the battery pack into the control module as far as possible as shown at left Push the wires fo the right of the battery as shown at right Replace the battery door and slide it closed FIGURE 2 2 INSTALLING THE BATTERY PACK If you have any questions regarding this procedure call Technical Support 800 543 4030 Basic Operation 3 EDP Plus can opera
29. nsed together Open VOLUME SEQUENCING by pressing F 7 SETTINGS 1 Display flashes Opl or volume last set Press number keys then E to set the first sequential volume V1 2 Display flashes Opl or volume last set Press number keys followed by E to set the next volume V2 Sequential volumes are numbered as follows V_g then Vacjq Vb 11y amp Vo 12 3 Continue in this way until all volumes are set Press 0 E to end the sequence OPERATION 1 The display shows the first pickup volume Press and release the trigger to pick up the first volume V1 2 Press and release the trigger again to pick up the next volume V2 Air gaps are not automatically added when VOLUME SEQUENCING If you wish air gaps can be set as volume entries 3 Continue in this way until all volumes are picked up 4 A three part tone sounds Press the trigger to dispense the total volume all at once 5 The display shows the first pickup volume again Press and release the trigger fo pick up volume V1 and restart the cycle 6 To close the VOLUME SEQUENCING function press F 7 This is a good way fo avoid the potential problem when using DILUTE mode with the 10mL liquid end that of the air gap rising through the diluent allowing diluent and sample to mix in the sample reservoir You can set a smaller air gap which will stay in place between the diluent and sample See Section 3 Basic Operation DILUTE mode 17 Prop
30. or proper sealing as described in the first part of this chapter 2 5 mL LIQUID END The 2 5 mL EDP Plus piston assembly threads into the shaft and is removed and replaced with a seal wrench Cat No 6100 231 Piston 1 Remove the liquid end from the control module 1 Assembly 2 Place the wrench in the slots in the piston assembly Turn the wrench counter clockwise to remove the piston assembly as shown in Figure 10 3 3 Clean or replace the seal as described earlier 4 Reinstall the piston assembly by first inverting the shaft and threading the piston assembly into the shaft Tighten firmly with the wrench Do not over FIGURE 10 3 tighten 2 5 mL LIQUID END REMOVING PISTON SEAL 23 5 Test operation by pushing and releasing the piston If the piston movement is not free or the piston does not return easily to its rest position the O ring and seal are probably not seated correctly In this case reinstall the piston assembly 6 Replace the liquid end on the control module 10 mL LIQUID END The piston seal is formed by a nitrile rubber O ring and fluorinated grease Refer to Figure 10 4 and proceed as follows 1 Remove the liquid end from the control module 2 Remove the upper portion of the liquid end Work the spring retainer beyond the threads of the shaft Carefully remove the piston assem bly spring guide bushing and O ring Discard the worn O ring 3 Remove all traces of used grease f
31. rage of the EDP Plus the Battery Pack should be fully charged and removed from the instrument NOTE If you use a Rapid Charge Stand to rest the instrument keep the Rapid Charge Stand unplugged until you need to recharge the batteries Recharging takes about 1 1 2 hours with the Rapid Charge Stand 28 Ordering Information B EDP Plus Pipettes parts and accessories can be ordered online 24 7 at www rainin com or by phone mail or fax from Rainin Instrument LLC Rainin Road Box 4026 Woburn MA 01888 4026 800 4 RAININ 800 472 4646 FAX 781 938 1152 e mail pipets rainin com 7500 Edgewater Drive Box 2160 oakland CA 94621 0060 FAX 510 564 1617 RAININ Japan 4 1 11 Hongo Bunkyo Ku Tokyo 113 Japan Phone 81 03 5689 8311 Fax 81 03 5689 2670 Other International See http Avww rainin global com list html or email global rainin com for the the name of the distributor in your country Complete EDP Plus Pipettes Includes one Control Module one Wall Power Supply and one Liquid End for the nominal volumes listed below Cat No Description EP 10 EDP Plus Pipette 10 microliter EP 25 EDP Plus Pipette 25 microliter EP 100 EDP Plus Pipette 100 microliter EP 250 EDP Plus Pipette 250 microliter EP 1000 EDP Plus Pipette 1000 microliter EP 2500 EDP Plus Pipette 2 5 milliliter EP 1OML EDP Plus Pipette 10 milliliter EP M8 10 EDP Plus M8 Pipette 8 x 10 microliter fixed liquid end EP M8
32. roduct Name EDP Plus Motorized Microliter Pipette Model Number EDP Plus Product Options Rapid Charge Stand Wall Power Supply conforms to the following Product Specifications Safety EN61010 1 1993 IEC1010 1990 A1 A2 EMC EN55011 1988 Class A Electromagnetic Interference EN50082 1 pr EN55024 2 1992 Electrostatic Discharge pr EN55024 3 1991 Electromagnetic Immunity pr EN55024 4 1993 Electrical Fast Transient Supplementary Information Responsible Signatory Haak Magnussen VP of R amp D Date August 30 1996 This Declaration of Conformity applies only to products which have the CE mark attached RAININ Rainin Instrument LLC 7500 Edgewater Drive Oakland CA 94621 3027 a METTLER TOLEDO Company Prices and specifications are subject to change without notice Copyright 1989 2002 Rainin Instrument LLC 9920 055 Rev H
33. rom the shaft interior guide bushing and piston assembly with a lint free wipe DO NOT USE SOLVENTS 4 Smear a light coat of fresh fluo rinated grease uniformly around the piston ensuring that no dirt is present Smear a light coat of grease on the new O ring and guide bushing 5 Place the O ring then the guide bushing small end of the bushing down on the shelf inside the shaft body Place the spring on top of the guide bushing Upper Shaft Body ad Piston Assembly Seal Spring Guide Bushing O Ring FIGURE 10 4 10 mL LIQUID END ASSEMBLY 6 Wipe any foreign particles from the front surface of the cone Carefully insert the piston into the shaft body and snap the spring retainer down beyond the shaft threads CAUTION BE CAREFUL NOT TO SCRATCH THE PISTON 7 Replace the upper body of the liquid end taking care not to cross the thread or overtighten 24 Troubleshooting 11 Symptom Possible Cause Suggested Remedy Inaccurate liquid measurement or leaks Bad seal between disposable tip and liquid end Use RAININ disposable tips Damaged shaft end Replace shaft Inadequate seal on piston assembly Check clean or replace piston seal as outlined in Section 9 Lob on display warbling alarm Insufficient charge in Battery Pack Recharge Battery Pack see Appendix B Blank display no alarm EDP Plus is shut off Press any top
34. s E 7 200i Wa MIX VOLUME ENTERED OPERATION 1 PIPETTE mode Press and release the trigger to aspirate the pickup volume Press the trigger again to dispense DILUTE mode Press and release the trigger to pick up the diluent Press and release the trigger to pick up an air gap and again to pick up the sample When the three part tone sounds press the trigger once more to dispense diluent and sample together The mix volume may be larger than the pickup or diluent sample volumes 14 2 Hold the trigger either during or after the dispense stroke to start mixing Mixing will continue as long as the trigger is held in The display alternates between PICKUP and DISPENSE while mixing is in progress DISPENSE V V 200l 200yl MIXING IN PROGRESS MIX VOLUME ALTERNATELY PICKED UP AND DISPENSED PICKUP 3 Release the trigger Mixing stops and a normal blowout stroke occurs The display shows the pickup or diluent volume ready for another cycle 4 To close the MIX function press F 6 VOLUME SEQUENCING F 7 in PIPETTE MULTIPLE DISPENSE and DILUTE modes VOLUME SQUENCING PIPETTE MODE In PIPETTE mode up to twelve distinct volumes can be picked up and dispensed sequentially with VOLUME SEQUENCING When using this function you are not restricted to the fixed sequence by pressing the F 0 keys you can go back and repeat the previous volume or by pressing E you can skip over the next volume The
35. ssistance Outside the U S A contact the RAININ distributor for your country If you do not know the address call International Customer Service at 001 510 564 1614 or send e mail to global rainin com RAININ distributors are also listed by country on the web www rainin global com list html 26 Performance Specifications 12 These manufacturer s specifications should be used as guidelines when establishing your own performance specification in accordance with ISO 8655 Table 12 1 Specifications EDP Plus VOLUME INCREMENT ACCURACY PRECISION Model SET uL ul h uL ul lt 10 pl 1 0 01 2 5 0 025 1 2 0 012 1 5 0 075 0 6 0 03 0 1 0 0 1 0 4 0 04 25 uL 2 50 0 05 6 0 0 15 2 0 0 05 2 5 1 2 0 15 0 4 0 05 25 1 0 0 25 0 3 0 075 100 uL 0 0 1 3 0 0 3 1 0 0 1 50 0 8 0 4 0 2 0 1 100 0 8 0 8 0 2 0 2 250 uL 25 0 5 2 0 0 5 0 6 0 15 125 0 8 1 0 0 15 0 19 250 0 8 2 0 0 15 0 38 1000 uL 100 1 0 3 0 3 0 0 6 0 6 500 0 8 4 0 0 13 0 65 1000 0 8 8 0 0 13 1 3 2500 ul 250 5 0 3 2 8 0 0 8 2 0 1250 0 8 10 0 0 16 2 0 2500 0 8 20 0 0 12 3 0 10ML 1 mL 10 5 0 50 0 0 6 6 0 5 mL 1 0 50 0 0 2 10 0 10 mL 0 8 80 0 0 16 16 0 Specifications are subject to change without notice and apply to PIPETTE mode operation 27 Recharging the Battery Pack A Power is supplied to the EDP Plus by either a wall power supply cord or with an accessory Battery Pack A fully charged Battery Pack will allow you to make more than 10
36. te in five modes MODE KEY DESCRIPTION P PIPETTE F A single volume is picked up and dispensed M MULTIPLE F 2 A single volume is picked up and multiple DISPENSE volumes dispensed stepwise T TITRATE F 3 A single volume is picked up and partially dispensed under trigger control D DILUTE F4 Diluent and sample are picked up separately and dispensed together MEAS F5 A sample is picked up under trigger control MEASURE and its volume is displayed Table 3 1 EDP Plus Basic Operation Modes are seletced in EDP Plus by holding the F key and i E i i a number key as shown in the table above The keyboard i shows the name or initial of the mode See sketch to right The instrument powers up in PIPETTE mode You can change modes when 1 The piston is in the HOME position and KB is displayed If necessary press the FUNCTION key F and then 0 to clear the current operation and move the piston to HOME KB should then appear on the display 2 The keyboard is not locked Press F 9 to unlock To change modes press F then the appropriate number key For example to open TITRATE mode press F 3 For MULTIPLE DISPENSE mode press F 2 The EDP Plus pipette has been designed to be easy to use whether you are right or left handed The balance weight comfortable feel and light touch of EDP Plus make it ideal for continuous use Hold EDP Plus loosely as shown in Figure 3 1 You do not need to gr
37. the piston The linear actuator controlled by a miniature onboard computer moves the piston the correct distance at the correct speed every time Piston strokes are consistent and reproducible Each complete EDP Plus pipette includes a control module a wall power supply and a replaceable liquid end The control module is the same for all volume ranges an encoder plug tells the control module which liquid end is attached Extra liquid ends with encoders can be purchased separately A precisely machined chrome plated stainless steel piston is moved by an electronic linear actuator The piston displaces a column of air which picks up or dispenses liquid through a disposable polypropylene tip This tip is the only part of the instrument that touches the liquid A mechanical tip ejector allows tips to be changed easily without hand contact avoiding sample carryover and contamination You enter mode volume and speed settings into the Encoder Plug microcomputer through a keyboard Operational status and settings are shown on an easy to read LCD liquid crystal display Audible tones signal completion of piston strokes and error conditions Pickup and dispense are performed by pressing the trigger Power can be supplied to the EDP Plus pipette in either of two ways with a cord attached to a wall power supply or with an rechargeable Battery Pack for maximum convenience and freedom of movement Tip Ejector Button Keyboard With a full
38. y charged Battery Pack installed the EDP Plus will operate for more than 1000 full stroke cycles FIGURE 1 1 before recharging is needed EDP PLUS CONTROLS AND INDICATORS The piston in the 10 mL liquid end is polished stainless steel not chrome plated Except 2 5 mL and 10 mL liquid ends Unpacking and Set up 2 UNPACKING In addition to this manual your EDP Plus package should contain 1 EDP Plus Electronic Pipette 2 Wall Power Supply Before using your EDP Plus inspect each component for damage sustained in transit If you find damage file a claim immediately with the shipping carrier Save the shipping container until any damage claim is resolved and the instrument is checked and operates properly WARRANTY Complete and return the Warranty Registration Card All EDP Plus Electronic Pipettes carry a one year warranty against defects in materials and workmanship Physical and chemical abuse are not covered For information regarding the warranty or repair of any instrument please call RAININ at 800 543 4030 e mail pipets rainin com www rainin com Outside the U S A local warranty conditions may apply Contact your Rainin distributor see Wwww rainin global com or e mail global rainin com NOTE If the equipment is used in a manner not specified the protection provided by the equipment may be impaired Wananty retums are only accepted with prior authorization SET UP Connect the wall power supply to a pow
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