NeuroWare® User`s Manual
Contents
1. Dongle should be indicated by Silicon Labs CDC Serial port COM under COM and LTP Version 1 5 6 03 26 2015 OPTOSTIMWARE User s Manual a Y Ports COM amp LPT J Communications Port COM1 aa Intel R Active Management Technology SOL COM3 al Silicon Labs CDC Serial port COM10 Figure 7 Information in Device Manager Version 1 5 7 03 26 2015 OPTOSTIMWARE User s Manual TBS Software Navigation Launch the OptoStimware software application OptoStimware is in Run mode once launched _ gt L A Figure 8 OptoStimW are Launch Screen Loading Saving Configurations Default parameters are loaded into OptoStimWare from the configuration ini file located on your computer gt C 2Ch OptoStimware Default Config File OptoStimware ini Version 1 5 8 03 26 2015 OPTOSTIMWARE User s Manual lt ui t Computer OS C 2Ch OptoStimware V1 7 0 4 Search 2Ch OptoStimware VI P Mame Date modified Type 2CH OptoStimware Setting 9 22 20151 44 PM Configuration sett 2CH OptoStimware V1 7 0 9 14 2015 2 33PM Configuration sett 9 22 2015 1 44 PM Filename Default Config File OptoStimware Figure9 Loading Configuration File Customized pattern parameters can be saved by pressing the Save As button The new configuration file by default will be saved to C 2Ch OptoStimware Figur2. 9 22 2015 2 40 PM Select HS 3 Downloaded i d 9 22 2015 2 38 PM 3 HSACK 9 22 2015 2 38 PM Search HS 3 Downloaded 9 22 2015 2 38 PM 2 HS ACK z 9 22 2015 2 38 PM Search HS 2 Downloaded Be AIANDI N m Figure16 Multiple Headstage Detection 3 Generate and download pattern in OptoStimWare Choose the stim signal parameters you wish to download to the headstage Refer to the Programming the Stim Channelssection of this document for more pattern programming instructions When finished click the Download Pattern button to send the pattern data to the headstage Opto Channell y Channel2 v Search HS Setup l mA S Stim Plr Ware Initial Delay ID E a a re Once following trigger Pulse Current PIL Pulse Duration PD1 SE us 7 A 50 A 10 ms z C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Train Multiple Pulses Train Frequency TF Turn On HS Turn Off HS 20 Hz Train Duration TD LED Alert Reset Alert _ Stimulus Multiple Trains Stimulus Frequency SF nAnNDS wv VIL Stimulus Duration SD Dongle Headstage Pattern 9 22 2015 2 56 PM Pattern Ack a 9 22 2015 2 56 PM Pattern Downloaded d 9 22 2015 2 55 PM Select HS ACK 9 22 2015 2 55 PM Select HS 2 Downloaded Quit Stimware 9 22 2015 2 55PM 2 HSACK 9 22 2015 2 55 PM Search HS 2 Downloaded MAar Ae Figuret7 Download and Trigger Iumination Patte
3. Figure24 LED Detection 4 Turn OFF Headstage Press the Turn Off HS button in StimWare to turn off headstage when experiment is over The Headstage indicator will go gray when headstage is off Version 1 5 19 03 26 2015 OPTOSTIMWARE User s Manual 9 22 2015 3 33 PM Off Command Ack 9 22 2015 3 33 PM Off Command Downloaded 9 22 2015 3 33 PM Trigger Command Ack 9 22 2015 3 33 PM Trigger Command Downloaded 9 22 2015 3 33 PM Trigger Command Ack 9 22 2015 3 33 PM Trigger Command Downloaded arn mn Figure25 Turning Off Headstage Version 1 5 20 03 26 2015 OPTOSTIMWARE User s Manual Application Notes Charging the Headstage Lithium lon Battery The wireless stimulator headstage includes an integrated rechargeable battery which should be recharged when not in use Recharging is accomplished by the following procedure 1 Connect supplied charging plug to the charge port on the headstage 2 The LED on the charging plug will turn on Troubleshooting Problem The headstage won t turn on Suggestion Charge the battery via the charging plug provided with the system Refer to the section titled Application Notes for charging instructions Problem One or more output signals are missing or incorrect Suggestion Download the stim signal parameters to the headstage with OptoStimWare making sure to follow all instructions within this manual Check to make sure that the channels are enabled w
4. illuminated and you will see warning information in log section If both LED are damaged you will see warning LED1 LED2 Voltage Abnormal If Ch1 LED is damaged and Ch2 LED is fine you will see warning LED1 Voltage Abnormal and must disable Ch1 if you still want to ran pattern on Ch2 LED If Ch1 LED is fine and Ch2 LED is damaged you will see warning LED2 Voltage Abnormal and must Version 1 5 18 03 26 2015 OPTOSTIMWARE User s Manual disable Ch1 if you still want to ran pattern on Ch2 LED Opto Stim Ware Channell 7 Channel2 7 SearchHS Setup l Initial Delay ID Once following trigger Pulse Current PIL Pulse Duration PD1 k l Aso us y so J0 ms x m C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Turn On HS Turn Off HS Download Tri Pattern nager Train Multiple Pulses Train Frequency TF SEJ Hz Train Duration TD eee eee Fae AS S zl TD _ Stimulus Multiple Trains Stimulus Frequency SF 10 002 LED Alert Reset Alert Stimulus Duration SD 1 500000 05 ms v ee o l l sD 9 22 8015 3 29 PM LED1 LED2 Voltage Abnormal 9 22 2015 3 29 PM Trigger Command Ack 9 22 2015 3 29 PM Trigger Command Downloaded 9 22 2015 3 29 PM Pattern Ack 9 22 2015 3 29 PM Pattern Downloaded 9 22 2015 3 28 PM Trigger Command Ack ARANAIS An Me Dongle Headstage Pattern o 2s m
5. minimum which is 50uS It can however be used to Stagger the stim channels so that only 1 channel is stimulating at a time Pulse Current PI1 PI is the amount of current driven through the OPTRODE LED The lower the PI the less current is driven through the LED The dimmer the LED Illuminates 100 13 32 mW at 10 mA Version 1 5 10 03 26 2015 OPTOSTIMWARE User s Manual Light Power 50 of Current Figure 12 Power by Current Percentage Graph Pulse Duration PD1 PD is the time that the LED is on The shorter the PD the less current is driven through the LED over whatever period of time that is set by the TF Together PD and TF make up the Duty Cycle that the LED is driven The greater the Duty Cycle the longer the PD the brighter the LED will illuminate 2 O 100 01 54 75 e7 50 14 25 a o 5 jla 0 20 40 60 80 100 Duty Cycle Frgure 53 Temperature by Duty Cycle Graph Train Frequency TF TF sets the number of individual pulses that occur per second and is measured in Hz Hz 1 Time Train Duration TD TD sets the total duration of stimulation event and is measured in microseconds milliseconds or seconds Version 1 5 11 03 26 2015 OPTOSTIMWARE User s Manual AN Stimulus Multiple Trains SF or Stimulus Frequencyis actually the length of time from the start of the Train to the end of 1 cycle By setting this number low relative to t
6. Opto Stim G2 Ware Wireless Optical Stimulation User s Manual a division of Harvard Bioscience Inc Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 Fax 919 544 3061 www trianglebiosystems com OPTOSTIMWARE User s Manual Table of Contents Document CVS VN ace sens wets E cae acne wn ee E E E E en 2 1 alll 8 54 81 S Ac Geen ee ee eo ee ee ee eee 2 Headstage PINOUT sssrin EEE EEE 3 UE SD Er rac a wists rrsie hs tec E sea neces waren E necioss 4 Me OFS CEO Fr ose cae cent acon a E E EE 4 Hardware VIN Cell ACL OIM ce sercen states gasna see ese e esterase E sess eeseseemcenaatae ss 4 SOMW Ah INS tat OM seese E E E 5 Minimum System Requirements ccccccccseccseccsecccecceuccenceeuceencceneceseeeeeceeeceeeeeeeeteeeeeeeteeetens 5 OptoStimWare Installation cc cccccececccseccceececeeeceecseeecseeeseeeeeseueceeeueceseueseeeneeeegness 5 Soltware IN ACO tga seca saan sceceecadsnnscosiecacnossacsrosasdedeensasanaseutesesoentecnsaneceasecics sen inucosasneedecssnanececencaasassiouienen 8 Loading Saving Configurations cccccccccccccccccccccccecseseeececceccccscseseeeeeuuueuesssseceeeeeeecssseeeeeeuauuugesseeeeeeeeeeeess 8 Programming the SUM Channel esssssensrn amen oe eee ne nt ene ae ner er er eo eee 10 PUI LCA WED sitashcccseainmsisadictnsta E uasiensbacs cence E E uaiaieaecsanamsbateasecrationa 10 PAE COre
7. e 10 Saving Parameters Version 1 5 9 03 26 2015 OPTOSTIMWARE User s Manual IN Programming the Stim Channels The two stim channels can be enabled and programmed separately from each other To enable a channel select the checkbox next to its name A channel that is unchecked will not output any signal so it may be advantageous to disable an unused channel to conserve battery life In the screen under the Channel tab you will see the items that define the stim pulse by setting the following settings Opto hannel 1 Channel 2 SearchHS Setup mE C Stim a5 Ware Initial Delay ID PD1 Once following trigger Pulse Current PIL Pulse Duration PD1 k l i50 us z150 2110 ms Jo s d fo Jo msi f C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Train Multiple Pulses Train Frequency TF Jo e Train Duration TD Download Z j _ Stimulus Multiple Trains LED Alert i Stimulus Frequency SF 10 0025 Stimulus Duration SD 1 400000 05 s E a j m z Dongle Headstage Pattern lll lll lll Figure 11 Distinguishing Separate Channels The graphics in the OptoStimWare interface are intended as conceptual references and do not reflect the actual pattern set by the user defined parameters Initial Delay ID ID is the user defined delay between the trigger and the start of the stimulation pulse In most circumstances this should be set to the
8. e Headstage If you have multiple headstages with different address you will be able to control each headstage with OptoStimWare At one time dongle can only communicate with one headstage Once the headstages are charged Set up Start Address and End Address based on headstage s address range Click the Search HS tab Press the Search HS button on that window and you will see your headstage listed in Available Headstages table Once your headstages are visible click on headstage that you want to control with mouse Press the Select HS button The green Headstage indicator at the bottom will illuminate and elected headstage address will be shown right inside indicator Version 1 5 13 03 26 2015 OPTOSTIMWARE User s Manual Opto Channel1l v Channel2 v Search HS Setup Stim Ware Dongle Version te a T r o ee ae Mi 23 0 Step1 Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS q noe me Step4 Select HS in list with mouse A 2 G 3 and click on Select HS Step5 Download pattern to selected ASilable Headstages ee ee cs Battery Status C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Turn On HS Turn Off HS Download Tri Pattern ngger LED Alert Reset Alert Dongle Headstage Pattern 9 22 2015 2 40 PM Select HS ACK a
9. he train duration low because it is the reciprocal of time you can create a blank period between stimulation trains SF lt 1 TD or Train Duration Example If TD 5S SF can be equal to or less than 0 2Hz Certain parametric limitations are programmed into the software to ensure signal consistency and prevent saturation of the headstage amplifiers If a parameter has exceeded its allowed range ex current percentage is too high it will automatically be reset to its highest allowed value PI1 range is 1 to 100 Typical current for the LED is from 5mA to 10mA Current for custom LEDs can be as high as 50mA Rossi MA Go V Murphy T Fu Q Morizio J Yin HH 2015 A wirelessly controlled implantable LED system for deep brain optogenetic stimulation Frontiers in Integrative Neuroscience 9 8 1 7 System Setup and Testing 1 Connect USB Dongle to computer Open the OptoStimWare program application If the dongle is successfully recognized by your computer the Dongle LED will illuminate in OptoStimWare Opto Channel 1 l Channel 2 Search HS Setup l Stim Ware Dongle Version eerie a Stepl Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS For ine Step4 Select HS in list with mouse op 2 and click on Select HS Step5 Download pattern to selected Available Headstages icshrsborcmebosenboalee HS Address HS Type Battery Statu
10. iebiosysiems com www tranglebosysioms com Figure 1 Images of Items Headstage Pinout OG oc oO lt JO I Black Dot LED Side Figure 2 Pin Mapping Version 1 5 3 03 26 2015 OPTOSTIMWARE User s Manual LED Optrode Diagram 8 00mm iien LED Positions LED Size 24W x 32L x 14H 1 38mm 7 2 36mm 0 6 mm Thickness Froure 3 LEED Optrode Diagram Also Available in 4mm Length Mating Connector e Omnetics A70010 801 Hardware Installation Screw the USB Dongle Antennae onto the USB Dongle Connect the LED Optrode to the Headstage ensuring the alignment of pins and omnetics logo 3 It is recommended to charge the Headstage until the indicator light on the Charger goes from red to green Version 1 5 4 03 26 2015 OPTOSTIMWARE User s Manual Computer Opto StimWare Software Omnetics Connector 24 GHz A ni mal Headstage Optrode Trigger 1 Trigger 2 External Stimulation Triggers TTL Pulse Figure 4 System Setup Diagram Software Installation Minimum System Requirements e Atleast 1 0 GHz Processor e 2GBof storage memory e 1GBRAM e 1 USB 2 0 port e Windows 7 OptoStimWare Installation To install and run OptoStimWare 1 Insert the OptoStimware installation disc into your computer Navigate to OptoStimware Installer folder within the installation disc and double click setup exe then follow the prompts until installat
11. ion is complete Version 1 5 5 03 26 2015 OPTOSTIMWARE User s Manual IN 2 Navigate to the Silabs CDC_Install folder within the installation disc and double click Silabs CDCInstaller_x64 exe if Operation System is 64bit or Silabs CDCinstaller_x86 exe if Operation System is 32bit then follow the prompts until installation is complete GO di lt EFM32 USB driver Silabs CDC Install Organize Include in library Share with Burn New folder sir Favorites Name Date modified Type E Desktop dpinst 3 25 2014 3 05 PM XML Document 11 KB a Downloads C Silabs_License_Agreement 8 16 2013 11 55 AM Text Document 7 KB S Recent Places Ef silabs cde 4 10 2014 3 50PM Security Catalog 9 KB 3 Dropbox 4 4 201411 50 AM Setup Information 3 KB E 10 24 2013 11 39 Application 1 026 KB Libraries Si CDCInstaller x8 10 24 2013 11 39 Application 901 KE Documents a Music E Pictures E Videos hb 6 items Figure 5 Select dongle driver 3 Plug the USB dongle into PC Silicon Labs CDC Serial port driver will be installed Silicon Labs CDC Serial port COM10 installed Silicon Labs CDC Serial port COMO wf Ready to use Figure 6 Dongle driver installation done 4 Right click on My computer and select Manage to open the Device Manager If you do not have My computer on your desktop click the start button then right click on Computer and select Device Manage
12. ithin the software prior to downloading Check all equipment used to monitor the signal to ensure continuity FAQ To see a large series of questions and answers regarding the ontogenetics systems please view this link Call TBSI customer support at 919 361 2663 if there are any problems with system setup and function You can also submit a Technical Issue report on our website here Version history for this manual 1 1 Initial release with Specifications 12 13 1 2 Added pattern creation description 5 14 1 3 Added Search HS function 1 4 See Scanned Changes file 3 30 1 5 Overhauled Version 1 5 21 03 26 2015
13. n R 76 E 10 PULE DU On POT aee E E E E TAE E EEE 11 Tran Freguency TF rreri E EEA 11 TaN O OAT D aaa E T 11 Stimulus Multiple Trains arseen E N O EE 12 System Setup and Testing oirin irren enr EEE E E E E 12 PO DIC AU OM NO SE AEE EEA A 21 TOUDE HOONE aee E On eee eee eer errr 21 FO eC EE A ee ee ee 21 Version history TOF this MANU scsscucctesscnvesdannavcsesnanesseoncnesaesacdenedanseesid soap uncteesndededsanseadanntesudenannssausancedeunars 21 Version 1 5 1 03 26 2015 OPTOSTIMWARE User s Manual Document Overview This document will demonstrate how to use the TBSI OptoStimWare hardware and software The program controls a single 2 channel headstage that allows researchers to generate and download two separately customizable waveform patterns via a wireless USB dongle The headstage is battery operated therefore only needs to be connected to the charging plug for recharging Shipped Items List e 2 Channel Optogenetic Stimulation Headstage e OptoStimWare Installation CD e USB Dongle e Headstage charger e Trigger Cables e Optrode Headstage USB Dongle Optrode Version 1 5 2 03 26 2015 OPTOSTIMWARE User s Manual Trigger Cables Charger Installation CD Opto Stim gF Ware Neuro Stimulating Software for Bio Research 2012 Triangle BioSystems international inc 2224 Page Road Suite 108 Durham NC 27703 919 361 2663 T 919 544 3061 F sales triang
14. nd and should be connected with the ground of external system All external trigger settings are located in Set up tab of StimWare as Figure16 shows Either rising edge or falling edge can be selected as active trigger edge The channel should be disabled to avoid false trigger when you finish experiment or when you don t use external trigger Trigger setting won t take effective until you click Set Up External Trigger button Version 1 5 15 03 26 2015 OPTOSTIMWARE User s Manual Opto Channell Vv Channel2 v Search HS Setup Stim E Ware External Trigger Setting Set Up Ch1 Enable Chi Edge Selection External Trigger i Rising Edge x Ch2 Enable Ch2 Edge Selection C 2Ch OptoStimware V1 7 0 i v Rising Edge 7 4 Default Config File OptoStimware ini Turn On HS Turn Off HS Download Tri Pattern ngger LED Alert Reset Alert 9 22 2015 3 03 PM Set Trigger Ack 9 22 2015 3 03 PM Set Trigger Downloaded 9 22 2015 3 01 PM Trigger Command Ack 9 22 2015 3 01 PM Trigger Command Downloaded 9 22 2015 2 56 PM Pattern Ack 9 22 2015 2 56 PM Pattern Downloaded An Nnr narr me sI ar Dongle Headstage Pattern Waj gt Figure 19 External Trigger Setting When valid external trigger is detected it will trigger the pattern loaded in headstage Trigger Cable will show up in log window and Pattern indicator starts blinking as Figure17 shows Op
15. rn Version 1 5 14 03 26 2015 OPTOSTIMWARE User s Manual SEN 3 1 Software Trigger Press Trigger to start the pattern The Pattern light will illuminate and blink while the pattern is running and will stop blinking when pattern is over Opto Channel 1 Channel2 V SearchHS Setup n ee Stim GE Ware mosso G as Once following trigger Pulse Current PIL Pulse Duration PD1 k l 50 50 10 cc om o gt all C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Train Multiple Pulses Train Frequency TF A20 Hz Train Duration TD hom DEARER EZERS Rae peel E _ Stimulus Multiple Trains Stimulus Frequency SF 0 0025 Stimulus Duration SD 1 400000 05 ms 9 22 2015 3 01 PM Trigger Command Ack 9 22 2015 3 01 PM Trigger Command Downloaded 9 22 2015 2 56 PM Pattern Ack 9 22 2015 2 56 PM Pattern Downloaded 9 22 2015 2 55 PM Select HS ACK 9 22 2015 2 55 PM Select HS 2 Downloaded AIA PInAaer Wee a Dongle Headstage Pattern a oe Figure 88 Triggering Pattern 3 2 External Trigger Two external triggers are supported As Figure1 shows one end of trigger cable can be plugged in dongle and the other end are two bare wires that can be connected with external system in which external trigger signals are generated Red wire is external trigger signal whose voltage is up to 5V Black wire is grou
16. s C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Download Tri Pattern ngger LED Alert Reset Alert Dongle Headstage Pattern 9 22 2015 2 05 PM Dongle Detected A a Figure 64 Dongle Recognition Version 1 5 12 03 26 2015 OPTOSTIMWARE User s Manual AN 2 Search and Turn ON Headstage Once the headstage is charged Click the Search HS tab Press the Search HS button on that window and you will see your headstage under Available Headstages Once your HS is visible press the Select HS button or Turn On HS and the green Headstage light at the bottom will illuminate and turn on Headstage Opto Channel V Channel 2 Search HS Setup l Stim Ware Dongle Version eneen Scary HS renner Mazo Stepl Power on all HS for example 6 HS Step2 Check Coninuous Search and Set up Start Address and End address Step3 Click Search HS Step4 Select HS in list with mouse and click on Select HS Step5 Download pattern to selected HS or send cmd to it C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini Download Tri Pattern ngger LED Alert _lll Battery Status 9 22 2015 2 16 PM Select HS ACK A Dongle Headstage a 9 22 2015 2 16 PM Select HS 2 Downloaded i Sele ownloade 9 22 2015 2 15 PM Dongle Detected Figure 7 Search and Select Headstage Multipl
17. the reasonable pattern duration by considering T4 which is interval between the two consecutive trigger pulses Trigger Signal VL Pattern Start Pattern Stop R l Rising edge Figure 21 External Trigger Rising Edge Active e Falling edge active If Falling Edge is selected in Ch Edge Selection the falling edge of external trigger signal will trigger pattern If the negative pulse T1 is more than 4ms it s regarded as valid trigger The pattern will start after delay T2 If the negative pulse T1 is less than 4ms it s regarded as false trigger and ignored and the pattern won t start T3 is interval between the end of pattern and the next coming trigger edge User should set up the reasonable pattern duration by considering T4 which is interval between the two consecutive trigger pulses Version 1 5 17 03 26 2015 OPTOSTIMWARE User s Manual 1 T1 lt l l VH Trigger Signal i l VL Pattern Start Pattern Stop d A Falling edge Parameter Description VH External Trigger High External Trigger Low D ef a a ee ee wooo end to next trigger pattern E E time m _ E Figure 23 External Trigger Table LED Detection When you start Pattern either by Trigger button in OptoStimWare or external trigger on dongle the status of LED will be detected before pattern is running If optrode isn t there or LED is burned out LED Alert indicator will be
18. to Channeli y Channel2 v Search HS Setup rae mA a Stim a5 Ware Initial Delay 1D Once following trigger Pulse Current PIL Pulse Duration PD1 pare 50 us M 50 A 10 ms z iE C 2Ch OptoStimware V1 7 0 4 Default Config File OptoStimware ini V Train Multiple Pulses Turn On HS Turn Off HS Download Pattern ligan LED Alert _ ll Reset Alert Train Frequency TF J i 20 Hz Train Duration TD H500 ms B Stimulus Multiple Trains Stimulus Frequency SF Ap Ans Vv VL Stimulus Duration SD A Ann A rA SUUUUU 05 ms k 9 22 2015 3 06 PM Trigger Cablel a 9 22 2015 3 06 PM Trigger Cablel 9 22 2015 3 06 PM Select HS ACK 9 22 2015 3 06 PM Select HS 2 Downloaded 9 22 2015 3 06 PM 2 HSACK 9 22 2015 3 06 PM Search HS 2 Downloaded e AIARA ke Dongle Headstage Pattern a fe Figure 20 External Triggering Version 1 5 16 03 26 2015 OPTOSTIMWARE User s Manual IN e Rising edge active If Rising Edge is selected in Ch Edge Selection the rising edge of external trigger signal will trigger pattern If the positive pulse T1 is more than 4ms it s regarded as valid trigger The pattern will start after delay T2 If the positive pulse T1 is less than 4ms it s regarded as false trigger and ignored and the pattern won t start T3 is interval between the end of pattern and the next coming trigger edge Users should set up
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