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Gynkotek HPLC pomp P580 manual ENG

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1. 4 4 5 Programming e Choose the item Programming from the main menu to change to the programming mode P 580 Main Menu Status Display Programming Setup Diagnosis Note The possibilities described here are intended for stand alone operation If the P 586 is connected with CHROMELEON or GynkoSofi ihe pump is controlled via the data system see sections 4 6 and 4 7 The programming mode allows you to enter 10 different operating programs and to save them under a free number Each program can comprise a maximum of 40 program steps The programs are stored even if the pump is switched off In this mode you can edit delete and overwrite pre viously stored programs In this mode only the left hand keypad is active Select Program free get free free set idle active set free set e Use the arrow buttons lt 4 I A Y to select the desired program number for the program The currently selected program is underlined The status set marks the programs created in the 33 Gynkotek HPLC Operating instructions P 580 programming mode Pressing the key 1 activates a set program The active program is then performed by pressing the Start key Free program locations are available for creating new programs e Pressing the key 2 defines a set program as the flush program indicated by the program type idle This program is started automatically after the active program is completed To prevent starting the flush program
2. Diagnosis displays information relevant for any service or repair work Also this menu allows changing the language setting for the display In the submenu the currently used solvent types can be specified P S80 Main Menu Status Display Programming Setup Diagnosis e n the event of an error please write the displayed information down for the Gynkotek HPLC Service Diagnosis Firmware Rev XXXX Work Load XXXX Uncalib Press xxxx Cursor up Language 38 Gynkotek HPLC Operating Instructions P 580 The last line language allows you to change the language setting for the pump display be tween English and German via the arrow key A In the Diagnosis menu press Enter again to select the currently used solvents Solvent Types Water Methanol Automatic Custom Proceed as described in section 3 10 to set the solvent types you are using Press Esc to return to the main menu 39 Gynkotek HPLC Operating Instructions P 580 4 5 Special Aspects of the High Pressure Gradient System The high pressure gradient pumps P 580A HPG P 580M HPG and P 580P HPG operate with two pressure pump sets integrated in a single housing Operating the high pressure gradient pumps is identical to operating the low pressure gradient pump However please note the fol lowing items 4 5 1 Supported Gradient Combinations If using the optional Solvent Selector 2 from 4 the channels A and C are connected to the pump
3. Start and Stop buttons differs from the usual func tionality in gradient programming Defining custom solvent types is not possible while a gradient program is active Therefore terminate the gradient program prior to defining custom solvent types 24 Gynkotek HPLC Operating Instructions P 580 4 Operation 4 1 Front Panel Display and Control Elements Programming a amp tup Diagnosis i i lipas Puree T Pus oa e 3 4 5 7 8 9 10 12 13 14 15 16 17 18 Fig 15 Display and Control Elements on the Front Panel shows the current pressure shows the upper and lower pressure limit shows the portion of the selected mixing channel shows the current status the individual menus the programs and the individ ual program steps PER Lyset jit he seed program ____ A 7 to the sune ordinate menu activates the display or input of individual mixing channels corresponding LED is active Pressing a button for approx 2sec sets the corresponding por tion to 10055 activates the pressure display input of the upper and lower pressure limit cor responding LED is active uw res e LED Flow On and stops LED Flow Off the flow 1 P Not available in manual operation 25 Gynkotek HPLC Operating Instructions P 580 4 2 Safety Measures Caution Caution Caution Caution Caution Caution Caution Note Caution When operating the HPLC system always ensure
4. 1022 Wire label 3mm white B 2175 1032 Wire label 3mm white C 2175 1042 Wire label 3mm white D 4820 2550 Operating Instructions P 580 English 2309 1100 Container for Accessories 59 Gynkotek HPLC Operating instructions P 580 I EEr Accessories for Pump 1310 7031 Power cord 220 V 3 x 0 75mm 2m P 580 I O connecting cable 25 pin Fuse 0 5A 5 x 20 mm for 230V Fuse 1A 5 x 20 mm for 100V Silicone wash tube 2 80x1 30 EDxID 1 m Suction tube P 580 preparative without fnis Capillary connecting tube stainless steel 1 16 ID 0 70 1 m complete 2281 2001 Plastic syringe 12 mi 5007 9302 Tool for preparative pump 6005 1301 Cap seal for purge valve screw Wire label 3mm white A Wire label 3mm white B Wire label 3mm white C Wire label 3mm white D Operating Instructions P 580 English Container for Accessories 10 Teflon solvent filters Knurled fitting 1 8 PEEK Clamp ring PEEK for tubing 3x1 5 Support flange PEEK for tubing 3x1 5 Wire label 3mm white A Wire label 3mm white B Wire label 3mm white C Wire label 3mm white D 15025 9515 Accessories for Gradient Pump LPG and HPG complete 5025 2520 Suction tube P 580 ID 1 5 mm with teflon solvent filter p bi p p p pd pb pid a pi p pai p j d y 5025 9030 Accessories for Pump P 580 PEEK analyt and prep including 1310 7031 Power cord 220 V 3 x 0 75mm 2m Fuse 0 5A 5 x 20 mm for 230V Fuse
5. 1A 5 x 20 mm for 100V Silicone wash tube 2 80x 1 30 EDxID 1 m Suction tube P 580 ID 1 5 mm with teflon solvent filter PEEK capillary tube 1 16 x 0 25mm 1m Gynkotek HPLC Operating instructions P 580 Partno Description Quantity 5025 2515 Connecting tube 4 way valve pump head left ID 1 0 mm 12200 5502 Single part hand tight fitting 2261 0203 Knurled fitting 1 8 PEEK 2261 1602 Clamp ring PEEK for tubing 3x1 5 2261 1603 Support flange PEEK for tubing 3x1 5 6005 1301 Cap seal for purge valve screw 2200 0011 10 Teflon solvent filters Plastic syringe 12 mi Operating Instructions P 580 English Tool for analytical pump Container for Accessories p e ho jo do o MM mos m Models P 580A HPG Bio P 580A LPG Bio P 580P HPG Bio P 580P LPG Bio Part no Description Quantity 5025 9030 Accessories for Pump F 580 PEEK analyt and prep including Power cord 220 V 3 x 0 75mm 2m 1 1310 7031 1 1270 5003 Fuse 0 5A 5 x 20 mm for 230V 1271 0003 Fuse 1A 5 x 20 mm for 100V 12250 3011 Silicone wash tube 2 80x1 30 EDxID 1 m 5025 2520 Suction tube P 580 ID 1 5 mm with teflon solvent filter PEEK capillary tube 1 16 x 0 25mm 1m Connecting tube 4 way valve pump head left ID 1 0 mm Single part hand tight fitting Knuried fitting 1 8 PEEK Clamp ring PEEK for tubing 3x1 5 Support flange PEEK for tubing 3x1 5 Cap seal
6. 9001 P 580 VO connecting cable 25 pin 11270 5003 Fuse 0 5A 5 x 20 mm for 230V 1271 0003 Fuse 1A 5 x 20 mmy for 100V 12250 3011 Silicone wash tube 2 80x1 30 EDxID 1 m 5025 2520 Suction tube P 580 ID 1 5 mm with teflon solvent filter 56 Gynkotek HPLC Operating Instructions P 580 5020 9110 Capillary connecting tube stainless steel 1 16 ID 0 25 mm I m complete 5025 2515 Connecting tube 4 way valve pump head left ID 1 0 mm 2261 0203 Knurled fitting 1 8 PEEK Clamp ring PEEK for tubing 3x1 5 Support flange PEEK for tubing 3x1 5 Plastic syringe 12 ml Tool for analytical pump Cap seal for purge valve screw 10 Teflon solvent filters Wire label 3mm white A Wire label 3mm white B Wire label 3mm white C Wire label 3mm white D Operating Instructions P 580 English Container for Accessories Accessories for Gradient Pump LPG and HPG complete Suction tube P 580 ID 1 5 mm with teflon solvent filter UE LEM MEE M um Accessories for Pumy 1310 7031 Power cord 220 V 3 x 0 75mm 2m 8025 9001 P 580 VO connecting cable 25 pin 1270 5003 Fuse 0 5A 5 x 20 mm for 230V 1271 0003 Fuse 1A 5 x 20 mm for 100V Silicone wash tube 2 80x1 30 EDxID 1 m Suction tube P 580 preparative without frits Capillary connecting tube stainless steel 1 16 ID 0 70 1 m complete Plastic syringe 12 ml Tool for preparative pump Cap seal for purge valve screw 2175 1012 Wire label 3mm white A 2175
7. Measures eene nennen nee nnennee nentes en oett onset stans ones nasse cene tenen enneonce eae DO 4 3 Choosing the Solvents ccesssessssssresssorssssncseronessseperseregessssecasanepssssenseesscenaness paseesneceaces 26 4 4 Stand alone Operation ccccssssesssssscreccsrccrssansreeseseusenseeegsssseccenensngeassesseccepenseasgeesseceqens LO 4 4 1 Switching on the Pump MRNA 4 4 2 Status Display eere eere neenon einen nnn neenon tasse senes assa eeenenees s LY iti Gynkotek HPLC Operating instructions P 580 4 4 3 Manual Input eese HRS MER 30 4 4 4 LCD Display Menu Structure ccsssssssesscersnssenssessseeensesensenrssseesenssesegeanessnerenees 32 4 4 5 Main Menu secesusnsconttnsssagecensecancessrecaessseanneccenanaes OEE DO 4 4 6 Programming M QR JD 4 4 7 Gradient Programming Example MRNA peser DO 4 4 8 Setup SER EM MERE MEMINI MEM SRI 4 4 9 Diagnosis sesseeontnacesscenepacearsesnacessens ER E 4 5 Special Aspects of the High Pressure Gradient System sese sesevecttensanseteess 40 4 5 1 Supported Gradient Combinations O FO 4 5 2 Double Flow Mode set conanseeeenaees secensnsreuecscassassessesaacossessarenssesssees FU 4 6 CHROMELEON Control
8. for purge valve screw 10 Teflon solvent filters Plastic syringe 12 ml Operating Instructions P 580 English Tool for analytical pump Container for Accessories Accessories for Gradient Pump LPG and HPG complete Suction tube P 580 ID 1 5 mm with teflon solvent filter umi 2251 6001 5025 2515 ed 2200 5502 p p M M um 5025 2520 51 Gynkotek HPLC Operating Instructions P 580 8 2 Optional Accessories Part no Descripton V 1 1 Comment 15025 0500 Option Injection valve stainless steel with accessories 709 8125 20 RH8125 Injection valve complete for stainless steel pumps 709 3725 RH372351 Injection valve preparative for preparative pumps 1713 80865 Syringe 500yl for preparative pumps 1713 80565 Syringe 50yl Capillary cutting tool Knurled fitting Peek 1 16 Zero modem cable RS unit cable 9 pin 62 Gynkotek HPLC Operating instructions P 580 8 3 Spare Parts Models P 580A P 580A LPG P 580A HPG Part no Descrpion 1270 5003 Fuse 0 5A 5 x 20 mm for 230V 1271 0003 Fuse 1A 5 x 20 mm for 100V 8025 9001 P 580 I O connecting cable 25 pin 1310 7031 Power cord 220V 3 x 0 75 mm gray or black 2 m 1384 0308 Magnetic stirrer 8x3mm PTFE 2073 0161 Knurled screw M3 x 16 DIN 464 A2 for housing 2074 0161 Knurled screw M4 x 16 DIN 653 A2 for pump block cover 2116 2702 Pre
9. manual Replace solvent Replace detector lamp Purge the system Purge pump check replace check valves as necessary Degas solvents and check degasser Check setting of manual precompression see section 3 10 Shorten capillary or use ap propriate capillary Rinse or replace column Use fresh solvent Gynkotek HPLC Operating instructions P 580 Remedial action Analysis is not reproducible Sample is unstable and de Use new sample or change composes conditions Gradient is not reproducible Check pump function and degassing Exchange suction frits Ensure constant temperature and air humidity Use col umn thermostat Air bubbles in the system Purge the system Environment conditions are unstable Precompression display shows sudden fluctuation at constant operating condi tions Pump displays message Solvent supply is used up Refill solvent and purge Min Pressure Shutdown pump see section 4 4 3 3 Air bubble in suction tube Check filter frit purge system Solvent emits gas when Degas solvent and check de mixing gasser Leakage in system leaking Find leak tighten fitting fitting connection connection Check valve defective Clean replace valve and purge pump as necessary Contaminations on column Rinse or replace column Pump displays message Max Pressure Shutdown Minute parts from vial septa Find blo
10. may vary c g for pumps without gradient option not all descriptions necessarily apply to the delivered instrument At various points throughout the manual messages of particular importance are indicated by Note Caution or Warning The relevance of these is as follows Note Indicates general information to assist optimum performance to be obtained Caution Indicates that failure to take note of the accompanying information may result in damage to the instrument Warning Indicates that failure to take note of the accompanying information may result in per sonal injury 1 2 Unpacking and Checking Contents To unpack the unit proceed as follows e Place the box on the floor and remove the white accessories pack and the power cable e Pull out the unit slowly and carefully Place the unit on a table Caution To prevent the unit from falling hold the unit itself on both sides not the packaging material Now remove the packaging material and the polythene bag Note Retain all original packaging material in a safe place It is the optimum packaging for shipping the unit e g for repair Shipping the unit in any other packaging automati cally nullifies the warranty All materials used for the packaging can be recycled Check off the contents of the accessory pack against the list in section 8 1 In section 2 3 you will find a list of alternative equipment Gynkotek HPLC Operating Instructions P 580 1 3 Warranty The st
11. must be active during the input e With the left Clear button you can undo the last not yet confirmed input Note During operation the set pressure limits become immediately active Note To view the current system pressure in the pressure display press the Max Min but ton until neither Max nor Min LED are lit If the operating pressure of the pump reaches values outside the set pressure range the pump per forms the following steps The flow is stopped LED Flow Off is on the LEDs A B C D MIN and MAX blink e the error status is indicated on the LCD display e the error is reported to CHROMELEON or GynkoSoft as necessary Example When the pressure is below the specified limit the following error message appears Internal Error Low pressure shutdown CLEAR or FLOW Following a pressure limit shut down proceed as follows e Confirm the error by pressing Flow to immediately resume operation LED Flow On is ac tive e Confirm the error with one of the Clear buttons Remedy the cause of the excessive or low pressure see section 5 The pump is then ready to operate again The pump can be restarted by pressing the Flow button LED Flow On is active Note In CHROMELEON or GynkoSoft confirm the error via the displayed dialog win dow 31 Gynkotek HPLC Operating instructions P 580 44 3 4 Purge Use the Purge button to activate the Purge function i e to temporarily deliver with a high flow rate e g to r
12. w x h x d Weise o 12 7 15 8 kg depending on the model Technical information November 1998 subject to change without notice 57 Gynkotek HPLC Operating Instructions P 580 8 Accessories and Spare Parts 8 1 Standard Accessories Modeis P 580A EE Part no Descriptton Quantity 15025 9010 Accessories for Pump P 580 stainless steel ana lytical complete including 1310 7031 Power cord 220 V 3 x 0 75mm 2m 8025 9001 P 580 I O connecting cable 25 pin Fuse 0 5A 5 x 20 min for 230V Fuse 1A 5 x 20 mm for 100V Silicone wash tube 2 80x1 30 EDxID t m Suction tube P 580 ID 1 5 mm with teflon solvent filter Capillary connecting tube stainless steel 1 16 ID 0 25 mm I m compiete Connecting tube 4 way valve pump head left D 1 0 mm Knurled fitting 1 8 PEEK Clamp ring PEEK for tubing 3x1 5 Support flange PEEK for tubing 3x1 5 2281 2001 Plastic syringe 12 mi 5007 9301 Tool for analytical pump Cap seat for purge valve screw 10 Teflon solvent filters Wire label 3mm white A Wire label 3mm white B Wire label 3mm white C Wire label 3mm white D Operating Instructions P 580 English Container for Accessories LJ ME MM eM uu Models P 80A HPG P 580A LPG P 580M HPG Description Quantity Accessories for Pump P 530 stainless steel ana lytical complete including 11310 7031 Power cord 220 V 3 x 0 75mm 2m 18025
13. 20 1 000 20 1 000 20 Time 6 00 12 00 15 00 Program Step 5 Programming Flow Min Max 1 000 20 200 1 000 20 200 1 000 20 200 Time 12 00 15 00 28 00 c enum program Programming Flow Min 000 20 000 20 Time 000 20 000 20 000 20 the following appearance 37 29 00 Programming B c 55 0 0 0 55 0 0 0 80 0 0 0 Programming Time R C 6 00 55 0 0 0 12 00 80 0 0 0 100 0 0 0 15 00 Programming Time HB C 12 00 80 0 0 0 15 00 100 0 0 90 100 0 0 0 Programming Time 3B eC 00 00 00 00 00 Gynkotek HPLC Operating Instructions P 580 e Press Esc to return to the program list Use the arrow keys to select the program you wish to perform Press the key 1 to activate a previously edited set program The active pro gram is executed by pressing the Start button e Press ESC again to return to the main menu 4 4 8 Setup The menu item Setup serves to display the current pump configuration Warning Do not change any values in the Setup menu Any settings must be performed by the Gynkot ek HPLC Service Wrong input will cause serious errors Warranty cov erage shall be invalidated in the event of inappropriate changes P 580 Main Menu Status Display Programming Setup Diagnosis e Inthe event of an error please write this information down for the Gynkotek HPLC Service e Press ESc to return to the main menu 4 4 9 Diagnosis The menu item
14. 4 7 Gradient Programming Example The example below shows a binary gradient A water B acetonitrile This typical applica tion for a gradient pump serves to separate 16 PAH s of the EPA Priority Pollutant List Please note that the system must be equilibrated at the start time injection time Resetting the condi tions to the start values must be performed after completing an analysis Example e Stepl Time 0 min Total flow imi min Solvent A 45 Solvent B 55 Maximum pressure 20 MPa 200 bar Minimum pressure 2 0 MPa 20 bar e Step2 Time 6 min Total flow Imi min Solvent A 45 Solvent B 55 e Step 3 Time 2min Total flow 1ml min Solvent A 20 Solvent B 80 e Step 4 Time 15min Total flow Iml min Solvent A 0 96 Solvent B 100 e Step5 Time 28 min Total flow Iml min Solvent A 0 Solvent B 100 Gradient Profile 6 9 12 15 18 21 24 27 30 t m ini Fig 17 Gradient Profile Program Step 1 Programming B C D Programming Time Flow Min r m cT m kh 0 00 1 000 2 0 55 0 0 0 0 0 36 Gynkotek HPLC Operating Instructions P 580 Program Step 2 Programming Flow Min 0 00 1 000 20 6 00 1 000 20 Programming B C 55 0 0 0 55 0 0 0 Program Step 3 Programming Flow Min 20 6 00 1 000 20 12 00 1 000 20 Program Step 4 Programming Flow Min 1 000
15. G esee enero LO 3 5 1 Connections of the Integrated Online Degasser ecce eere eene ss LA 3 6 High Pressure Gradient System HPG NER 15 3 6 1 Fluidic Connections of the High pressure Gradient System ss 16 3 7 Installing the Optional Syringe Loading Sample Injector eee 17 3 8 Installation under CHROMELEON MM VEM AS 19 3 8 1 Required CHROMELEON Modules crece esee eee ee sees enses sesso ssassess LO 3 8 2 Installation in the CHROMELEON Installation Program 19 3 9 Installation under GynkoSof Lisuieei eren nene nnne nne rennen nnne eene emnes Del 3 9 1 Required GynkoSoft Modules eerie eere en eee nnn eene eene eene Dl 3 9 2 Installation in GSINST eee een ene eren renes nenne ns ann oes se sesseseses enne essne Zl 3 10 Selecting Solvent Types for Automatic Precompression Control 22 3 10 1 Selecting Solvent Types in CHROMELEON eene nete tne ntaineses 22 3 10 2 Selecting Solvent Types on the Instrument eene en 2D e 4 Operation SESE bibe RA EEE SHAE EEE ESSERE ESTE ERASE SERRE EE ERE SPSS ESE ERR SES EE EEE ESSE EE PRESSE Ee 25 4 1 Front Panel Display and Control Elements 0 0 ccssssssssesserersssssessessssssenersssesessesssses Dol 4 2 Safety
16. Gynkotehk Hic The Ultimate Chromatography Experience HPLC Pump Series P 580 Operating Instructions ius Lx Version 1 42 Date Nov 26 1998 4 Gynkotek HPLC A Division of SOFTRON GmbH Gynkotek Softron 1998 Doc P520 Ol E OPERINST 01 42 Gynkotek HPLC Operating Instructions P 580 Gynkotek HPLC Offices Headquarters Gynkotek HPLC A Division of Softron GmbH Dornierstra ie 4 D 82110 Germering Munich Tel 49 89 89468 0 Hotline 49 89 89468 444 Hotline e mail service gynkotek com Fax 49 89 89468 200 E mail gynkotek gynkotek com Branch Offices Gynkotek AG Trockentoostr 1 CH 8105 Regensdorf Zurich Tel 41 1 870 1212 Fax 41 1 870 1214 E mail gynkotek access ch Gynkotek SARL 3 Avenue Condorcet F 91260 Juvisy sur Orge Paris Tel 33 1 69560717 Fax 33 1 69444981 E mail gynkotek club internet tr Gynkotek UK Ltd 1 Maple Court Davenport Street GB Macclesfield Cheshire SK10 1JE Tel 444 1625 660 200 Fax 44 1625 660 209 E mail gynkotek gynkotek co uk Gynkotek HPLC Inc 19 Spear Road Suite 303 Ramsey NJ 07446 USA Tel 1 201 813 0806 Fax 1 201 818 0990 E mail oynkotek erols com Please visit our web site Aifp Avww gynkofek com Gynkotek HPLC Operating instructions P 580 DECLARATION OF CONFORMITY We Softron GmbH declare that the product Pump P 580 to which this declaration relates is in conformity
17. HPG systems it is recommended to determine a custom solvent type for each avail able solvent channel This information applies to all P 580 HPG models For high pressure gradient systems the precompression control of the pump is activated auto matically after the user specifies the currently used solvent types in CHROMELEON see 3 10 1 or via the front panel keypad see section 3 10 2 in stand alone operation Custom solvent types allow optimizing precompression settings for the currently used solvents This information applies to the models P 580A and P 580 LPG Precompression control for isocratic and low pressure gradient systems is fully automatic Use the default solvent type setting Automatic For information how to check this setting see the sections 3 10 1 and 3 10 2 Caution In contrast to high pressure gradients individual solvent types cannot be used for set ting the precompression on the models P 580 LPG and P 580A The reason for this is that the compressibility behavior of the delivered mixture can differ considerably from that of the individual components 3 10 1 Selecting Solvent Types in CHROMELEON To select the appropriate solvent types in CHROMELEON proceed as follows On the Control Panel e g P580Full supplied with CHROMELEON choose the Flow command from the Control menu e For the HPG models assign each available solvent A B C D depending on the model the appropriate solvent type from t
18. ID 1 5 mm 5025 2517 Connecting tube Valve B D Rear panel B D 5025 2518 Connecting tube Valve A C Rear panel A C 50252610 i
19. MELEON Installation program on the tab dialog box Error Levels See also section 3 8 2 Error Message Cause Remedial Action Driver and pump configu The selected solvent type Update the device driver ration don t match Solvent cannot be entered as the settings with the current type command discarded pump configuration was pump configuration Dou Use the Chromeleon Instal changed The device driver ble click the P 580 pump in lation Tool to adapt the settings no longer corre the CHROMELEON Instal driver configuration spond to the current pump lation Tool and confirm set configuration tings by pressing OK The pump configuration The pump configuration was Update the device driver has changed Use the Chro changed The device driver settings with the current meleon Installation Tool to settings no longer corre pump configuration Dou update the driver configura spond to the current pump ble click the P 580 pump in tion double click the pump configuration the CHROMELEON Instal name and press OK lation Tool and confirm set tings by pressing OK 5 2 GynkoSoft Error Messages Remedial Action Can t receive command re 1 Mains supply to pump is Eliminate cause enable sponse interrupted see atso error communication with Con No function nect Status timeout Communi Pump is not connected with Check cable connection and cation disabled too long T piece or COM interfa
20. Pa 25 bar during this phase correct pre compression settings cannot be ensured An error message will be displayed In this case increase the counterpressure e g by increasing the flow rate Wait again for ap prox 10 minutes before saving the precompression value by pressing Freeze 3 10 2 Selecting Solvent Types on the Instrument In stand alone operation or control via GyrkoSoft proceed as follows to select the appropriate solvent types on the front panel e Deliver the desired solvent at 10096 e Choose Diagnosis from the main menu Press Enter again in the Diagnosis view to reach the menu item Solvent Types e Use the arrow keys A V to select the desired solvent channel A D e With the model P 580 HPG use the arrow keys 4 gt to select the appropriate solvent type e If working with the models P 580 LPG or P 580A choose the solvent type Automatic see notes in section 3 10 e Repeat the above steps for each required solvent A D depending on the model e Press J Enter to save the solvent type assignment Caution For optimum results the solvent type settings of the HPG pumps must be adjusted to the actual solvents by using the commands Start Learn and Stop Freeze Follow the instructions below If HPG pumps are operated in the Automatic mode strong pulsation will result The Automatic mode is reserved for isocratic and LPG pumps Do not use this mode for operating HPG pumps F
21. Preparative version 477 8 ul per stroke Micro version 39 8 ul per stroke Analytical version 3 175 mm Preparative version 7 8 mm Micro version 2 25 mm Gradient formation Low pressure gradient quaternary High pressure gradient binary with optional Solvent Se lector from 4 solvents Flow gradient can be combined with soivent gradient Mixing chamber Standard volume 0 2 ml other volumes available upon request Maximum operating pressure Analytical version max 50 0 MPa 500 bar temporarily 100 0 MPa 1000 bar Micro version max 50 0 MPa 500 bar Safety features User Input Display Keypad 30 buttons 8 line LCD display 21 characters 11 LEDs solvent resistant Prog ing during active program temporarily 100 0 MPa 1000 bar I Remote Control All functions controllable via RS 232 interface permanent status transfer Preparative version max 15 0 MPa 150 bar Automatic load dependent work load Bio compatible vers max 20 0 MPa 200 bar Inputs outputs serial RS 232 interface 1 MSV 6 connection Piston diameter Minimum Maximum pressure shut down selectable Time monitoring of pump function 3 relay outputs 3 relay mputs for Start Stop Hold i i analog output for system pressure 56 Gynkotek HPLC Operating Instructions P 560 Stainless steel 1 4571 sapphire ruby ceramics UHM W ethylene PCTFE PTFE PEEK Full load 70W ames c 413 x 170 x 362 mm
22. al rear flushing chamber see 3 4 4 Buffers or solvents forming peroxide must be rinsed out after an analysis Alterna tively the pump can be operated at a low flow rate 0 05 0 1ml min This reduces the equilibration time of the column and the lamps of the connected UV or RI detectors when resuming operation Before switching from aqueous buffer solutions to an organic solvent the pump must be rinsed thoroughly with water When switching to other solvents ensure that the new solvent is miscible with the solvent still contained in the pump Otherwise the pump may be damaged s a guide for completely rinsing the P 580 incl degasser approximately 30ml rinsing volume per solvent channel are required At a flow rate 3 ml min this means that each channel must be rinsed for a minimum of 10 min If the pump flow is interrupted for longer periods the lamps in any connected UV or RF detectors must be switched off to prevent evaporation of the solvents in the flow cell The unit must be shipped in the original packaging Shipping the unit in any other packaging automatically nullifies the warranty 95 Gynkotek HPLC Operating Instructions P 580 7 Technical Information i Flow rate range Analytical version 9 001 ml min 10 ml min Micro version 0 0005 ml min 5 ml min Preparative version 0 005 ml min 100 ml min HPG Displacement volume working Analytical version 79 2 ul per stroke piston
23. andard warranty coverage for this unit is in accordance with the conditions of sale The warranty has a duration of one year from the invoice date and covers materials and labor ex Works Please note that wear parts cannot be covered by the warranty The warranty coverage shall become invalid in any case identified as resulting from inappropriate use service or the implementation of non specified spare parts Similarly the warranty coverage shall be invalidated in the event of inappropriate shipment packaging or failure to remove ag gressive or damaging solvent residues 1 4 Intended Use Depending on the model the P 580 pump is intended for the operation exclusively in analytical or preparative HPLC systems The pump can be operated either in stand alone mode or via the data systems CHROMELEON or GynkoSoft Please note that the unit may only be operated within its technical specifications see chapter 7 Gynkotek HPLC assumes no liability for material or immaterial damage resulting from misuse of the unit Gynkotek HPLC Operating instructions P 580 2 Overview 2 1 Unit Description The Gynkotek HPLC High Precision Pumps of the series P 580 are high quality modules for the HPLC analysis Whether as binary high pressure gradient system P 580A HPG with the op tional soivent selector 2 from 4 or as quaternary low pressure gradient pump P 580A LPG with the integrated degasser the technical specifications of the P 580 series fulfill t
24. ation 3 1 Positioning the Unit To position the P 580 pump proceed as follows 9 9 5 b Place the unit on a firm and vibration free surface Do not expose the unit to temperature fluctuations Do not expose the unit to direct sunlight Do not expose the unit to high humidity For safety reasons the solvent reservoir containers should be placed on the container tray on the P 580 pump The surface must be solvent resistant Notes The P 580 pump may be placed on top of the Gynkotek HPLC Auto sampler GINA 58 When the pump is in operation the stainless steel tray below the housing should be drawn out to catch any liq uid that may escape from the pump heads Fig f P 580 with container tray 3 2 Electrical Connections Unless otherwise specified Gynkotek MPLC instruments are factory set for operation at 230V SOHz If the local voltage differs from this setting the voltage selector on the rear of the unit next to the power socket see fig 2 and fig 4 must be adjusted To do this proceed as fol lows gt Disconnect the power cord Draw out the fuse cartridge using a small screwdriver fig 2 Draw out the small voltage selector board from the power socket housing Place the board with the label up below the drawing showing the required setting in fig 3 Turn the board so that the voltage value on the board corresponds to the drawing in fig 3 Adjust the plastic tab without turni
25. below the flow rate indicate s the current precompression The time in minutes refers to the run time of the current program 1f the pump is operated in manual mode the run time is not displayed In the lower right corner you can see the currently active program number Note The bar indicating the current precompression shows 0 to 100 precompression from left to right If for example water is delivered at 10 0Mpa 100bar 1 6 of the bar should be covered When system conditions are stable precompression should also be stable A suddenly increasing precompression value is an indication of air in the system Only during gradient steps a temporarily varying precompression value is normal due to the changed solvent properties Press ESC or Show to move from the status display to the main menu see section 4 4 5 Press the Start button to start the currently active program see section 4 4 6 29 Gynkotek HPLC Operating Instrucons P 580 4 4 3 Manual Input Note In this mode the buttons required for programmed operation Start Stop Del see section 4 4 6 are not available For a description of the keypad see fig 15 Display and Control Elements After switching on the pump flow rate input is enabled Use the numeric keypad to enter the re quired flow rate 4 4 3 1 Flow Rate adjustable in steps of 0 001 ml min e By pressing Flow you can start delivery with the currently set flow rate LED Flow On ac tive Pressing
26. block 1 left and the channels B and D are connected to the pump block 2 right see fig 13 The integrated Solvent Selector 2 from 4 supports the following combinations of binary high pressure gradients Pump biock 1 left Pump block 2 right i Solvent A Solvent B Solvent A Solvent D Solvent C Solvent B Solvent C u Solvent D In a specific time step each pressure pump block delivers only one of the two channels 100 It is not possible to specify a mixing ratio for the two channels connected to the same unit A and C B and D If a portion of the solvent A is set and then a portion of C is entered the portion of A is auto matically reduced to 0 The same applies to the channels B and D on the second pump set If portions of B and C are specified e g B 40 C 60 and then a portion of D 20 is entered the portion of C is automatically increased to 80 The same applies to all other channels 4 5 2 Double Flow Mode HPG models support the double flow mode This mode enables maximum flow rates of 20 ml min analytical version 10 ml min micro version or 100 ml min preparative version In standalone operation the double flow mode is available for each solvent combination and com position supported by the HPG pump The pump automatically calculates the flow maximum so that each of the two pump blocks delivers a maximum of 10 ml min analytical version 5 ml min micro version or 50 ml min preparative version Gy
27. ce enable communication via Connect Upper pressure limit ex Maximum pressure is ex Check the setting of the in ceeded ceeded jection valve and all other valves in the system Check the system for blockings by contamina tions Lower pressure limit ex Pressure falls below mini Check whether there are air ceeded mum pressure bubbles in the suction tube Check your HPLC system for leakage 46 Gynkotek HPLC Operating instructions P 580 6 Maintenance 6 1 General Notes The model P 580 is manufactured with high quality components and materials thus minimizing maintenance requirements Please follow the warning notes marked Caution to prevent damage and unnecessary wear The surfaces as well as the keypad and display are to a certain extent resistant to weak acids al kali and organic solvents Remove the solvents and other liquids immediately by absorbing them with a soft lint free cloth or paper do not rub If surfaces are exposed for longer periods these liquids can cause damage You can carry out the maintenance work described below Any repair work necessary beyond as well as regular checks to prevent soiling wear etc should be performed by the Gynkotek HPLC Service approximately once a year This is e g to prevent dry running of the camshaft If unex pected problems occur please contact your Gynkotek HPLC Representative For further information on shutdown and shipment refer to section 6 10 Caut
28. ces for the rear flushing unit point upwards see fig 18 e Tighten the pump heads with the I M4x40 screws e Insert the inlet and outlet valves into the working pump head In contrast to the inlet valve the outlet valve top has a conical cavity for the capillary connection The inlet valve bottom has a planar surface for the suction tube connection e Attach the capillary connections hand tighten and then tighten by a turn with a spanner size V4 e Attach the suction tube to the inlet valve again Take care to avoid cross threading e To avoid contaminations entering the HPLC system the pump must now be thoroughly rinsed minimum 30ml HPLC water or pure organic solution Open the purge valve to prevent rins ing liquid from entering the HPLC system e Test the pump for tightness and if necessary tighten leaking connections 6 6 Mixing Chamber with Pressure Sensor This information applies to all P 580 models Caution Do not loosen the retaining screw of the pressure sensor In any event of errors on the pressure sensor contact the Gynkotek HPLC Service 52 Gynkotek HPLC Operating Instructions P 580 6 7 Purge Valve Seal Replacement This information applies to all P 580 modeis Rinse toxic solutions from the pump e Set the pump flow to 0 Wait until the system is without pressure Remove the purge valve screw from the mix ing chamber unit Use a small flat screwdriver to remove the plastic cap seal
29. cked spot and clean block the system Use different septa Check crimper setting Check connection to MSV replace MSV as necessary see section 4 4 3 3 Connection to motorized switching valve is inter rupted or MSV is defective Pump displays message MSV not connected Deactivate the MSV in the Programming menu by en tering in corresponding program lines see section 4 4 6 Poor degassing degasser Leak in capillary or solvent Check capillary and solvent motor runs in short intervals tube connection tube connections for tight ness tighten fitting connec tions MSV switching is included in program steps but no MSV is connected Flow rate too high Reduce flow rate Mains supply to pump is Call service Do not touch interrupted the degasser switching power supply Degasser motor stops 44 Gynkotek HPLC Operating Instructions P 580 Remedial action Degasser motor runs without Leakage in system leaking Find leak tighten fitting intervals fitting connection connection check degasser Leak sensor detects leak er Leakage in system leaking Find leak tighten fitting ror message on display fitting connection connection see section 6 3 LEDs A B C D MAX MIN blink 45 Gynkotek HPLC Operating Instructions P 580 5 1 CHROMELEON Error Messages A complete list of CHROMELEON error messages for the P580 is included in the CHRO
30. connections handtighten plus an approx V5 tum If neces sary tighten leaking connections Only use the ready made capillary tubes included with the pump Only use capillary tubes that are cleaned inside If necessary shorten the capillary tubes with the cut ting tool for capillary tubes part no 2140 0001 Never use a file to do this Con taminants or filings even minute parts can cause damage in the HPLC system pump injection valve etc To connect the capillary tubes to an injection or switching valve use the ferrules and fitting screws in the accessories included with the valve Read the installation notes from the valve manufacturer Refer to section 3 6 to find detailed information on the instaliation of a high pressure gradient system For mixing the gradient a dynamic mixing chamber with a volume of 200p is inte grated in the high pressure part of the pump To meet individual requirements Gynkotek HPLC offers mixing chambers with variable volumes for flow rates above 3 5 ml min Please contact your Gynkotek HPLC distributor for further in formation 10 Gynkotek HPLC Operating Instructions P 8580 3 4 4 Piston Seal Rear Flushing The mode P 580 has a rinsable rear seal flushing chamber piston seal rear flushing To supply the chamber with rinsing liquid proceed as follows e Unfasten the upper silicone tube that connects the two pump heads and attach the free end to the plastic syringe supplied with acces
31. d Replacement 0 s cccsssseesseserescesseeeeesssassessssereees 52 6 6 Mixing Chamber with Pressure Sensor SNR senes eene sene sns eanssennessss J 6 7 Purge Valve Seal Replacement M HERR sbebaneneeeenees 53 6 8 Degasser oo sccccccsssstesssssesanessenssssessensneessones RR eene DO 6 9 Low Flow Connecting Tube Replacement eene c eeeeeen eene enne esneeeeetes JA 6 10 Pump Shut Down eee eee eeieeeeee ee ees sesta nata sn tarta s setate titt tests testet ets eeeseeseaasstes OF 7 Technical Information Pd REP RR ee Rd RR ee ER Wd RETRRRRRERER Pd dd bh bbhubRRREESTARRSRRRRERERRRERRRRTRRAR 56 8 Accessories and Spare Parts 1 eeeee eee aeree serene eerta setenta soonest sess satu so setees tasso esas cras JO 8 1 Standard Accessories VENE beseeeeesesseeeees MEME NUR 58 8 2 Optional Accessories MES MER MR OL 8 3 Spare Parts EMEN VERRE 263 Gynkotek HPLC Operating instructions P 580 1 Introduction 1 1 About the Operating instructions The layout of this manual is designed to provide quick reference to the appropriate sections ac cording to the operation required However it is recommended that before operating the P 580 this manual should be read thoroughly and completely in order to obtain full understanding of the instrument Note AS the device configuration
32. e vacuum pump runs without pauses or clearly over 50 of the time check the system for possible leaks When connecting the solvent tubes ensure that the connectors are free of contaminants Even with minute particles air can enter the degasser reducing the unit s effectiveness When replacing solvents ensure that solvents are miscible Solvents that are not miscible must be replaced step by step by mixing with an intermediate solvent e After operation thoroughly rinse with alcohol can stay in the degasser During longer periods of inactivity and when using saliferous buffers due to risk of salt crys tallization in the gas separation membrane and thus reduction of degassing performance rinse with de ionized water then rinse with methanol or 2 propanol 6 9 Low Flow Connecting Tube Replacement If the pump is operated with flow rates lower than 2 ml min the tube between the 4 way valve and the pump head ID 1 5mm can be replaced by a low flow connecting tube ID 1 0mm Part no 5025 2515 This will lower response times in gradient operation Proceed as follows to replace the tube t 9 9 6 If necessary rinse toxic solutions from the pump If the pump is connected to the CHROMELEON or GynkoSoft Data System terminate the communication with the data system via the Disconnect command of the Online Control window in CHROMELEON or GynkoSoft see corresponding Manual Switch off the pump Disconnect the power cord L
33. ek HPLC Operating Instructions P 580 3 3 Rear Panel Connectors No Descripion 3 Power switch n 2 _ Mains connection r P 250V fuses 0 5A for 230V USE ONLY WH e RLS il CECIEL PIA BETOME AERLICRG FUST system connection MSV Motorized switching valve control RS 252 VO Input Output for external control PRESSURE for external dis olay of th Fig 4 Rear Panel Connections 3 3 1 RS 232 Interface The P 580 pump can be controlled with the data systems CHROMELEON or GynkoSoft Con trol is via a RS 232 interface This interface has a 9 pin male connector on the rear of the instru ment Fig 4 Note For this connection use only the connecting cables supplied by Gynkotek HPLC This ensures trouble free operation of the connection The following cables are avail able e 9 pin connection Null modem cable part no 1310 2260 e 25 pin connection RS unit cable part no 8914 0103A shipped with the RS T piece Note For controlling the pump with CHROMELEON the pump is connected directly to the PC For the control via GyrkoSoft the P 580 pump is connected either directly to a serial PC interface e g COML or to a GynkoSoft Profibus T piece 3 3 2 l O Connector The digital L O connector of the HPLC pump P 580 see fig 4 offers the possibility to connect an autosampler or a hand operated valve for example This connector supplies the digital inputs Start Stop and Hold a
34. exceeded 3 3 3 Pressure Output The P 580 features an analog output for recording the operating pressure of the pump PRESSURE see fig 4 The pressure output is set to 2m V bar For monitoring the pump pres sure it is possible to connect a recorder or a CHROMELEON or GynkoSoft A D converter Pin Assignment 2 pin Connector ND e Outer ring GN a uu 3 3 4 Motorized Switching Valve MSV It is possible to connect a Gynkotek HPLC MSV 6 e g for column switching to the P 580 The corresponding connection is located on the rear of the pump see Fig 4 The control of the motorized switching valve is via pump programming or via the data systems CHROMELEON or GynkoSoft Caution Use only the Gynkotek HPLC MSV 6 motorized switching valve Gynkotek HPLC Operating instructions P 580 3 4 Fluidic Connections Caution The pump is primed with 2 propanol When operating the P 580 pump for the first time make sure that the solvents you use are miscible with 2 propanol Otherwise use appropriate intermediate steps 3 4 1 Connecting Solvent Supply When connecting the pump and the solvent storage containers please note the following e The supplied solvent tubes must be connected to the threaded adapters A to D depending on the model on the rear of the instrument e Threaded adapters that are not required must be closed with one of the supplied blind plugs Caution To draw solvents from the supply containers only use suction
35. from the purge valve screw Remove the ring seal e Prior to assembly the purge valve screw must be cleaned with a solvent in an ultrasonic bath e Place the new ring seal Part no 2266 0311 onto the purge valve screw Insert in the cor rect direction see fig 19 e Place the new cap seal Part no 6005 1301 into the cavity of the Assembly tool for cap seal included in the standard accessories e Now press the purge valve screw onto the cap seal until it locks into place Ensure that the screw is pressed onto the cap seal at the right angle e Screw the purge valve screw into the mixing chamber unit the first resistance is felt e Now tighten the purge valve screw by an ap prox 4 tum Do not overtighten e Finally check the purge valve for tightness If necessary tighten the purge valve screw Fig 20 Cap seal assembly again Caution Do not use any tools when tightening the purge valve screw If the purge valve screw is too tight the cap seal can be de stroyed 6 8 Degasser This information applies to the models P 580A LPG and P 580A LPG Bio e Clean the solvent tubes regularly Warning Do not open the vacuum chamber of the degasser Do not remove the silicone tubes to the vacuum pump o3 Gynkotek HPLC Operating instructions P 580 Note the operating noise of the degasser As soon as the operating vacuum is reached the vac uum pump is automatically switched on and off at intervals If th
36. he Type list on the Flow System tab e For the models P 580 LPG and P 580A choose the solvent type Automatic see notes in section 3 10 e Confirm your input by pressing OK Caution For optimum results the solvent type settings of the HPG pumps must be adjusted to the actual solvents by using the commands Learn and Freeze Follow the instruc tions below If HPG pumps are operated in the Automatic mode strong pulsation will result The Automatic mode is reserved for isocratic and LPG pumps Do not use this mode for operating HPG pumps 22 Gynkotek HPLC Operating instructions P 580 For optimum results proceed as follows to set custom solvent types on HPG models Deliver the desired solvent A B C or D at 100 flow 1ml min at approx 10 0 MPa 100 bar system pressure Caution The solvent must be delivered at 100 Otherwise the precompression value cannot be set correctly e In the CHROMELEON control panel choose the Flow command from the Control menu e Onthe Flow System tab assign the Custom solvent type e Choose Learn from the list of commands available for the pump During this phase the pump optimizes the precompression setting e Wait approx 10 minutes at 1ml min and then select the command Freeze to save the opti mum precompression setting Caution During the Learn phase the counterpressure should be at the maximum operating pressure If the pressure is lower than 2 5 M
37. he high require ments in reproducibility of the delivery rate zero pulsation operational reliability and economic efficiency The patented isokinetic pre compression allows a precise and zero pulsation flow With flow rates ranging from 1yl min to 1Oml min analytical version and operating pressures up to 50 0 MPa 500 bar the P 580 is suitable for both regular and micro HPLC The programming possibility of the P 580 pump which allows time programs for gradient and flow parameters and the control via relay and motorized witching valve provides high flexibility even in stand alone operation The P 580 pump can be controlled via the Data Systems CHROMELEON or GynkoSoft offering a high degree of system integration All parts contacting media are manufactured from stainless steel PCTFE PEEK sapphire etc which results in excellent resistance against most solvents and buffers The P 580 is also avail able in a biocompatible configuration 2 2 Principle of Operation The P 580 gradient pump is a zero pulsation serial dual piston pump with electronic compressi bility compensation The two pump heads of the P 580 are serially connected i e the solvent passes both pump heads working and equilibration pump head successively Continuous delivery is achieved as follows The working piston delivers at the appropriate flow rate while simultaneously filling the serially connected equilibration head The latter serves as a reservoir and co
38. imes with a dry and lint free paper towel to remove any cleanser from the pistons When replacing the piston ensure correct centering 6 5 3 Piston Seal Removal Each piston has two piston seals One seal is located in each pump head and the other is located in the piston seal rear flushing chamber Replace the seals in intervals of approx 4 months Pro ceed as follows e Use a disassembled piston to remove the piston seal from the pump head see fig 1a b Insert the inlet and outlet valve cartridges the wrong way into the disassembled working pump head arrows now pointing to opposite direction than in fig 1a On the equilibration pump head close the bore hole with a blind plug Insert the piston tip into the piston seal Due to the pres sure the seal will be loosened In general this seal can still be used for the rear seal flushing chamber Do not forget to remove the inlet and outiet valve cartridges and to re insert them in the right direction see arrows in fig 1a If it is not possible to remove the piston seal in this way use the tool available from Gynkotek HPLC pointed screw or a M4 screw e g used of Gynkotek HPLC Operating Instructions P 580 for holding the pump heads Insert the screw into the seal Remove the seal However this procedure destroys the piston seal Caution Together with the piston seal on the high pressure side of the pump always exchange the support ring spare parts kit P 580 piston seal s
39. immediately after the first sample the start impulse for the next sample should arrive before the end of the currently active program When using the flush pro gram it is therefore recommended to enter longer program times for the analysis samples than in the control program for the autosampler As soon as the sampler finishes the last sample and the pump does not receive a new start impulse the flush program is started Note The Start and Stop commands can also be selected in other modes e g while the Status display or the main menu are shown However the commands always apply to the currently activated program under Programming e Press Del to delete the selected program The status set now changes to free e Confirm the program number by pressing 1 Enter to access the programming mode Programming Time Flow Min 0 00 1 000 100 The currently editable program line is underlined To enter a new program line or to edit an existing program line scroll the display to move to the desired cell via the arrow buttons gt A and V Move to the Time column via the buttons A and Y Use the buttons and to reach the other fields e Use the numeric keypad to enter values e Press the left hand Clear bution to undo the last not yet confirmed value e After entering a value confirm your input by pressing J Enter The cursor then moves to the next input field in the active line e To correct the values in a program li
40. inse an air bubble out of the suction tube To activate the Purge function proceed as foliows Press the Purge button to start rinsing with the currently set mixing ratio e Press the Purge button again to deactivate the function Caution With the P 580 Micro model do not deliver more than Iml min with the Purge func tion as optimized capillaries are used for suction and delivery in this model Caution Use the Purge function only with the purge valve open Otherwise the sudden pres sure increase could destroy sensitive columns or cause leakage in the HPLC system Caution Using the Purge button you can rinse only small air bubbles out of the system To remove larger amounts of air from the suction tube use a svringe to draw the air via silicone tubing to the purge outlet see section 3 4 5 44 4 LCD Display Menu Structure P 586 Maln Menu C Program Steps Solvent Types Fig 16 Menu Structure e Press J Enter in the main menu to access the underlined submenu Press Esc to return to the main menu 32 Gynkotek HPLC Oparaling Instructions P 580 4 4 5 Main Menu In the Status display press ESC or Show to view the main menu P 580 Main Menu Status Display Programming Setup Diagnosis e Use the arrow buttons and V to select the individual menu items The selected item is un derlined e Press J Enter to change to the selected menu item Press Esc to return to the main menu from one of the submenus
41. ion The unit must be shipped in the original packaging Shipping the unit in any other packaging automatically nullifies the warranty When shipping the instrument to Gynkotek HPLC please do not forget to include the Service Return Form with a description of the problem and the Decontamination confirmation Warning The P 580 may not be operated with open case except if this is specified in the in structions The warning notes given in the instructions must be strictly followed Warning Please keep in mind that the fluidic components of the pump may be filled with toxic solvents Therefore you should purge the pump with an adequate solvent and wear protective clothing before starting maintenance or repair work on the pump Caution Use original Gynkotek HPLC spare parts only Should damage result from repairs with third party parts or accessories Gynkotek HPLC assumes no liability 6 2 Removing the Unit Cover Warning Switch the instrument off and disconnect the power cord before you remove the cover Components may carry current Loosen the two knurled screws on the rear of the instrument Slightly lift the cover on the back 1 2 cm Slide the cover to the front until the tabs at the front are released from the housing Now lift and remove the cover e 9 9 6 3 Checking Tightness Check the pump for leaks every day Tighten leaking tubing and capillary connections Check the stainless steel tray below the unit for moist
42. is connected with the GynkoSoft data system the keypad is locked The entire contro and programming of the pump is now performed via GynkoSoft The display will show the message Remote Control Active 42 Gynkotek HPLC Operating Instructions P 580 5 Troubleshooting Remedial action Baseline drift too strong Peak broadening Pump not connected to mains Power switch off Blown fuse Replacement fuse blows immediately Leakage in the system Double check valves incor rectly installed not in flow direction or defective Column contaminated System not sufficiently equilibrated Environment conditions un stable Solvent is contaminated Detector not warmed up Detector problem Solvent is contaminated Lamp is too old Air bubbles in the system Pressure fluctuations of the pump pulsation Capillary to detector is too long or has a large inside di ameter Column is overloaded or contaminated Alteration of the solvent Connect power cable Switch on power Replace fuse Call service Find leak remedy Install double check valves correctly see fig 18 re place as necessary Rinse or replace column Rinse system until equili brated Ensure constant temperature and air humidity Replace solvent and check filter frits In aqueous sol vents growth of microor ganisms cf 3 4 1 is possi ble llow full detector warm up time Refer to detector
43. ith solvent connectors A D M Shut off valves switching valves with Solvent Selector option L3 Sowenttubes A C B D 4 DE ee ELE ee ee Fig 13 Fluidic connections of the high pressure gradient system Caution The optional Solvent Selector 2 from 4 allows HPG models to deliver specific combinations of binary high pressure gradients Follow the instructions given in sec tion 4 5 16 Gynkotek HPLC Operating instructions P 580 3 7 Installing the Optional Syringe Loading Sample Injector To install a hand operated sample injector the upgrade kit Sampie Injector is available from Gynkotek HPLC Mount the injector to the P 580 housing as shown below No jPart No Description amp 1 j21000501 3 056 5053 S5 Retaining washer 3 2055 0301 Ji M x30 Screw h 5 5 EH AS Washer mm 5 i i iniecfc 50252004 ie illa tube B nnecfion Fig 14 Installing the optional syringe loading sample injector Proceed as follows gt Disconnect the pump from the mains Remove the top cover of the housing see section 6 2 Remove the two blank plugs on the right side of the lower housing section Insert the supporting rectangle block between the support column and the housing wall Fasten the mounting angie for the syringe loading sample injector with the two I M5x30 screws to the housing Use the supplied washer
44. jPumpbeck LR ET sHiCovercamehat s s 13 Gynkotek HPLC Operating instructions P 580 3 5 1 Connections of the Integrated Online Degasser The integrated online degasser is connected to the low pressure side i e the suction side of the pump See the following diagram for an overvtew Caution Caution Caution Caution A B C D OOS 3 Soventtibes SSS 4 Check vave 5 5 Working head front view amp a 6 Equilibrium pump head front view Fig 11 Solvent tube connections to the Degasser low pressure gradient system Do not connect the Degasser with the pump outlet Otherwise the degasser mem brane may get punctured When connecting the solvent tubes ensure that the connectors are free of contami nants Even with minute particles air can enter the Degasser thus reducing the unit s effectiveness Keep in mind that when purging the HPLC pump short term rinsing the system with increased flow rate see section 3 4 5 the flow rate increases considerably If the maximum flow is exceeded gas bubbles may form After switching on the pump the integrated online degasser requires approx 12 min utes to reach the optimum vacuum Please note that a sufficient period must be al lowed until the pump delivers with maximum degassing performance 14 Gynkotek HPLC Operating instructions P 580 3 6 High Pressure Gradient System HPG The HPG models are optimi
45. lable Parameters Indicates sets status on off Indicates sets opening closing duraton in seconds sec Turns relay on off For further information on controlling the instrument via CHROMELEON please refer to the CHROMELEON User Manual and to the context sensitive CHROMELEON Online Help 41 Gynkotek HPLC Operating Instructions P 580 4 7 GynkoSoft Control Note For details on installing the P 580 in a GymnkoSoft system configuration refer to sec tion 3 9 and to the GynkoSoft Reference Manual The following gives a short list of the available GynkoSeff control commands for pump control For a detailed description of the individual commands refer to the GynkoSoft Reference Manual e Connect Disconnect e Flow Total e B 96C Pressure Limits e Stop Flow Hold Via the GynkoSoft relay switching function the pump relays and the motorized switching valve described in section 3 3 4 can be controlled This requires the installation of the corresponding relays in GSINST see GynkoSoft Reference Manual chapter 2 The table below lists the as signment of GynkoSoft relay numbers to the corresponding pump functions Event 1 Event 2 Event 3 not availabte not available MSV INJECT LOAD Note The Stop Flow command stops the pump flow and the program time count the Hold command only stops the program time count the analysis conditions here mixing ratio and flow rate are frozen Note As long as the pump
46. ne overwrite them and confirm by pressing J Enter e To delete the current program line press Del while the Time field is underlined After completing the last column Max and pressing J Enter you can enter the desired mixing ratio In the programming mode the portion of solvent A cannot be entered directly but is the difference of the three other solvent portions from 106 A 100 B C 6 D If the entered percentages exceed a total of 100 the mixing ratio is automati cally corrected Programming 3D Gynkotek HPLC Operating instructions P 580 Note For high pressure gradient systems with the optional solvent selector please note the supported combinations of high pressure gradients see section 4 5 e After entering the mixing ratio it is possible to specify the status of the two relays RI and R2 and the motorized switching valve MSV If a relay or the MSV is not addressed the corre sponding columns must be marked by default setting In the case of the two relays 0 indicates the status Off and the entry 1 indicates the status On For the MSV 0 switches to Load the input 1 switches to Inject Programming Rl R2 MSV E amo m Note After processing the last program step the pump continues delivery with the current settings To automatically stop the pump flow upon program completion add the last program step with the flow 0 00 to the program Ca
47. ng the board as shown in fig 3 Ensure that the tab locks into place Slide the voltage selector board into the power socket housing as shown in fig 3 Now replace the fuse cartridge Finally check whether the correct voltage is set Caution Choose one of the settings shown in fig 3 100V or 230V Other settings are not supported 5 Gynkotek HPLC Operating instructions P 580 Caution When installing the instrument for the first time check the proper mains and earth connection of the unit For minimum interference effects all components of the analysis system should be connected to the same mains output Warning Prior to changing the voltage or replacing the fuse you must disconnect the power cord 3 2 4 Replacing the Fuse Disconnect the power cord Draw out the fuse cartridge using a small screwdriver fig 2 Replace the fuses Caution Use only fuses of the type specified in fig 2 min nominal voltage 250V or listed in the Accessories Original Spare Parts List If the voltage selector is set to 100V use 1A fuses For 230V you will need 0 5A fuses Replace the fuse cartndge 5x20mm DIN 41662 BOHM Pert Nov di m woe ROADS PUES zany ay ST tie dug Fig 2 Voltage selector and fuse replacement n EDU 1 Insert direction s ae Arrow on arrow 2 Insert voltage selector board in this direction 2 z pi ho lt a r spannu dheg Fig 3 Mains filter Gynkot
48. nkotek HPLC Operating Instructions P 580 4 6 CHROMELEON Control Note For details on installing the P 580 in a CHROMELEON system configuration refer to section 3 8 and to the CHROMELEON online Help and User Manual In CHROMELEON controlling the HPLC pump P 580 can be performed in two ways e directly via the toolbar the menu bar and the controls in the Control Panel viatime programs PGM File or command buttons Note During the control via the data system the keypad of the instrument is locked Con trol and programming the pump is now entirely via CHROMELEON The display will show the message Remote Control Active The following commands are available to display and change current pump settings 4 6 1 CHROMELEON Commands Connect Establish communication between data system and pump Connected Pump confirms connection to data system Disconnect Terminate communication with ihe pump Flow in ml min A 96B 6C 26D availability depending on the model value name solvent type Pressure Limits upper and lower limit current value If the values are outside these limits the sample batch is aborted Emergency handling is started Stop Flow Hold interrupt program Learn begin Learn mode to define custom solvent type Freeze end Learn mode to define custom solvent type t 5 9 6 4 6 2 Relays OperableOutRelay 3 Relay1 P580 Relay2 Relay2 P580 MotorValve Motor Switch Valve Avai
49. nveys while the working piston carries out the suction stroke The characteristic feature of the patented isokinetic precompression is the 120 degree overlapping phase of the de livery strokes of working and equilibration piston When delivering compressible liquids without controlled precompression the pulsation increases with increasing operating pressure since part of the delivery stroke is required for the compression of the solvent in the pump head Pulsation during the precompression phase is reduced to a minimum by velocity modulation of the drive The highly constant delivery is ensured by a patented secondary control system auto matic compressibility compensation The flow rate is always kept constant in relation to the at mospheric pressure For user input see section 3 10 Switching on the model P 580 LPG automatically activates the integrated Online Degasser It serves to continually degas the solvents via special polymer membranes permeable to gas but not to liquids The online degasser thus guarantees optimum operation regarding reproducibility and pulsation reduction Gynkotek HPLC Operating Instructions P 580 2 3 Supported Configurations The HPLC pump P 580 is available in various configurations The pump is suitable for numerous laboratory environments In addition to the isocratic and the quaternary low pressure gradient pump with an integrated degasser a binary high pressure gradient system is available optionally equip
50. o henol otassium dichromate otassium hydroxide otassium nitrate otassium permanganate ropane ropyl alcohol ure benzene alad oil ilicone oil odium chloride odium hydrogen carbona Sodium hydroxide Sodium hydroxide odium thiosulphate ulphur dioxide ulphuric acid ulphuric acid ulphuric acid 100 v o Ez e days GJ days days richloroethylene w sl al of 2 S121 218 A OT A a T pr rrv 3 3 rr Ss sts 3 xylene inc chloride e d a 28 Gynkotek HPLC Operating Instructions P 580 4 4 Stand alone Operation 4 4 1 Switching on the Pump Switching on the pump The following message temporarily appears on the LCD display P 580A HPG 4 Firmware Rev Offset DMS In addition to the model here analytical high pressure gradient pump with 4 solvents the dis play shows the firmware version and the offset value of the pressure display After approx 5sec the status display appears 4 4 2 Status Display The status display indicates the current operating settings of the pump You can also view this display by choosing the item Status Display from the main menu see section 4 4 5 Status tA amp B tC 100 0 0 0 0 0 Flow Pressure 1 000ml min 20bar Time 0 00 min Pgm L In addition to the currently set mixing ratio the display indicates the flow rate in milliliters per minute and the current pump pressure The horizontal bar s
51. o0 eccssscecessnecseressssssaseesssecceneessesssessesssnssnees MEME 4 4 6 1 CHROMELEON Commands seseannesesenatsecsesssennnesnsstsnesesssesenssesesesscescssssoecee Fl 4 6 2 Relays MEER MER NENNEN MAE 3 4 7 GynkoSoft Control MERE DEM PERRA eee 42 5 Trou bleshooting Loe ete ete REEERE A RTT I LL RARE ERE RR ee eR RR Rede de rd d dee 43 5 CHROMELEON Error Messages bescensensseeceees beccsseererenacaeeees pesetas natae ana ecesecesss F 5 2 GynkoSoft Error Messages MEME NEM SPEMNNMNMNMNMMMMM E t E 6 Maintenance bih RRT E E PEE EES SEE SEES ERS FRE SSS SSS HES E E ee ee ee eS SSE eS Ee ee a 4d PTPRR 47 6 1 General Notes eee MEE see eeseceanseeneses Y 6 2 Removing the Unit Cover se eeaeaneensones sseesussesenenentene Lsssasesserenenseaes levsesssesnenaeteees 47 6 3 Checking Tightness ccsscccscsssscsssescnsscscescnssssscnssssensensssseassesssencecsssssenesssseessensesacscees FH 6 4 Double Check Valve Replacement NEM MERE NERA SEE 6 5 Pistons and Piston Seals n m MEME NAT 6 5 1 Pump Head Removal eseesecseanetees sesensctensccee rene RS 6 5 2 Cleaning the Pistons een RR sesenenteestesecssnsseoeeesesee D L 6 5 3 Piston Seal Removai eeneerae rene PDEMIINEMHIRMMMM sessssseernaatacens ENDE 31 6 5 4 Piston Seal and Pump Hea
52. onfiguration Ciose the CHROMELEON Installation program 20 Gynkotek HPLC Operating instructions P 580 3 9 Installation under GynkoSoft Caution GynkoSoft supports controlling the low pressure gradient pump P 580 A LPG as a ternary gradient pump Quaternary gradients are not supported 3 9 1 Required GynkoSoft Modules In GynkoSoft a controlling timebase PCSx is required The control is via the RS 232 interface see section 3 3 1 3 9 2 Installation in GSINST Driver Installation To control the P 580 pump with GynkoSoft the driver for Gynkotek HPLC M480 must be in stalled via the installation program GSINST Note The P 580 communications parameter must be set to 9600 baud 8 data bits 1 stop bit no parity Note For a detailed description of the driver installation procedure refer to chapter 2 of the Gynkosoft Reference Manual 21 Gynkotek HPLC Operating instructions P 580 3 10 Selecting Soivent Types for Automatic Precompression Control For commonly used HPLC solvent types precompression values of the P 580 are already pre set at the factory see also section 2 2 Entering numerical precompression values manually is there fore net required for the P 580 The operator simply specifies the solvent types that the pump will deliver If the P 580 is connected to CHROMELEON the solvent types are selected in the data system see 3 10 1 Otherwise the types are selected on the front panel of the pump see 3 10 2 For
53. oosen the retaining screws on the pump s top cover and remove the top cover see section 6 2 Loosen the inner retaining screws see fig 10 of the main board lift and tilt the board to the outside Remove the connecting tube between the 4 way vaive and the inlet valve Now the low flow tube can be inserted Tighten the connections only partly Take care the PEEK seal of the tube is exactly parallel to the tube Avoid cross threading Hand tighten the connections Do not use any tools to do this Tilt the main board back and tighten the retaining screw Replace the unit cover and fasten Reconnect the power cord and switch on the pump Purge the pump see 3 4 5 if necessary use the Connect command to reconnect the pump to the CHROMELEON or GynkoSoft data systems see corresponding Manual 6 10 Pump Shut Down To shut down the P 580 e g for interrupting operation for longer than 12 hours or for shipment please take note of the following Caution The pump should never run dry Otherwise damage to the pistons or the piston seals may result Caution For transport or for longer interruptions the pump must be filled with methanol or a similar alcohol e g 2 propanol ethanol Ifthe solvents in the pump are not miscible 54 Gynkotek HPLC Operating instructions P 580 Cantion Caution Caution Note Caution Note with water replace the solvents by adequate steps Do not forget to fill the piston se
54. or optimum results proceed as follows to set custom solvent types on HPG models Deliver the solvent A B C or D at 100 flow t ml min at approx 10 0 MPa 100 bar sys tem pressure Caution The solvent must be delivered at 100 Otherwise an error message will be dis played 23 Gynkotek HPLC Operating instructions P 580 Choose Diagnosis from the main menu Press 4 Enter again in the Diagnosis view to reach the menu item Solvent Types e Use the arrow keys lt 4 to select the solvent type Custom e Press Start Learn The pump now determines the optimum precompression setting e Wait for approx 10 minutes at Iml min Then choose Stop Freeze to save the Cus tom solvent setting In the Solvent Types menu this status is indicated by the star symbol next to the Custom solvent type Caution After pressing Start the counterpressure should be at the maximum of the usual op erating pressure If the pressure is below 2 5 MPa 25 bar during this phase correct precompression settings cannot be ensured In this case the following error message will appear on the display Internal error Freeze not possible at current counterpressure or compressibility e If this error message appears increase the counterpressure e g by increasing the flow rate Wait again for approx 10 minutes before saving the precompression value by pressing Stop Caution Here the functionality of the
55. ped with the solvent selector 2 from 4 If required the binary high pressure gradient sys tem can be expanded to a quaternary high pressure gradient system bv connecting an additional high pressure gradient slave pump Additional flexibility is offered by the dynamic mixing cham ber with variable volume that can be adjusted to individual requirements The following list gives you an overview of the currently available models If you have any ques tions please contact the Gynkotek HPLC Sales Department or your Distributor Model PartNo _ Configuration Gradient Configuration Stainless steel 1 4571 isocratic pump analytical Stainless steel 1 4571 isocratic pump semi preparative PEEK isocratic pump analytical inert PEEK isocratic pump semi preparative inert Stainless steel 1 4571 quaternary low pressure gradient pump analytical 5025 0035 PEEK quaternary low pressure gradient pump analytic inert 5025 0016 Stainless steel 1 4571 binary high pressure gradient pump analytical 5025 0025 Stainless steel 1 4571 binary high pressure gradient pump semipreparative 5025 0036 PEEK binary high pressure gradient pump analytic inert 5025 0046 PEEK binary high pressure gradient pump semipreparative inert 5025 0050 Stainless steel 1 4571 isocratic pump micro 5025 0065 Stainless steel 1 4571 binary high pressure gradient pump micro Gynkotek HPLC Operating Instructions P 580 3 Install
56. ption For questions contact your Gynkotek HPLC distributor 3 8 2 Installation in the CHROMELEON Installation Program Proceed as follows to install the HPLC pump P 580 in the CHROMELEON installation program Start the CHROMELEON Server and then the CHROMELEON Installation program e Choose the timebase to which you wish to assign the P 580 e Choose Add Device from the Edit menu or the Context menu of the right mouse button e Select Gynkotek P 580 from the displayed list of supported instruments Confirm by pressing OK The now displayed tab dialog boxes show the configuration of the pump P 580 These settings usually do not require alterations However it is important to check whether the settings corre spond to the current installation environment If necessary change the settings Tab Dialog Box General The General tab allows you to define instrument and hardware parameters such as the installa tion name and serial communication port Option Deseriptin 1 1 Device Name Instrument name under which the HPLC pump is identified in the installation environment default Pump ISerial Communication Serial PC port to which the pump is connected Port The current port settings are displayed but cannot be edited baud rate data bits parity stop bits and handshake IDemo Mode Demo mode on off Tab Dialog Box Error Levels The Error Levels tab serves for classifying errors in specific e
57. r refractive index refractive index detector dis solved gas degasser 4 3 1 1 Chemical Resistance to PEEK Gynkotek HPLC Operating Instructions P 580 a 1 o an TER Re a a T Linni ee De m Dol airs ar e E LEL b el m Da mds a ta 23 acemET 0 00 TES moras lager d j LN CAE EE UE M PEE FEE mal Mag 7 a n H re 22 1 iLi 4 Ee a met EO PEU CER 3 z zi zi z z ee ea eee pk ma a 7 7 m m ol oo olololol ao ool ol ol olol orol ow alo a T oF SISI n fx ES enzcaic acid rake fluid romine utane utanol alcium hydroxide arbon dioxide Carbon tetrachloride hloric gas hlorine liquid hlerobenzene hloroform hromic acid itric acid opper ll sulphate yclohexane yclohexanot yclohexanone Detergent Diethyl ether Diisopropyl ether imethylformamide loctylphthalate loxan thanoic acid thanol Ethyl acetate Ethylene glycol erric chloride ormaldehyde ormic acid ruit juice Gear lubricant oil ready for use j days 23 6 cn 96 Vol Heptane ydraulic fluid ydrochloric acid ydrofluoric acid ydrogen peroxide ydrogen sulphide Kerosene Paraffin actic acid Magnesium chloride Methanol Methyl ethyl ketone Methylisobutyicetone o j a 3 J 35 5 E days m 00 Motor oil itric acid itic acid itrobenzene utrient fat 100 esl te h3 d Gynkotek HPLC Operating instructions P 580 i araffin oil erchlorethylene Phen
58. re rre rennen nnn HR NEMMIMMM praesen D 32 Electrical COMMECtIONS sccescscseessssreccsseesgeessnssccepesetsscessuseecessansesaseesseqeesenseceanpetsaeqersnte 2 32 1 Replacing the Fuse cccssscessecesceessssersereccsecnessssssecsasarecsessessecenssavserssacsesesersars O 3 3 Rear Panel Connectors eee e eee eee eee i eeee sas as ettet attt disssseeeeeseesat toas eesassseeeesaass T 3 3 1 RS 232 Interface c ccsrsseccesssrececescnrscssansceesevcarssvevessnsoonntscssocnscoonssssssscsnsssesssvsare T 3 3 2 VO COMMECTOL ERR 3 3 3 Pressure Output Lisdsseceeeeneeeessssseesenssseeeesseeseesensaseeeesssssedsissneeeressasiesnsnees 3 3 4 Motorized Switching Valve MSV s cccscssssssssrsssssenssssesecersussesnsssersesersssssssnsse D 3 4 Fluidic CoOnnectiomS ccssscssesssssneenssssessessseressesseeeessessssessesseceresensersssssereesssssessonsens D 3 4 1 Connecting Solvent Supply esee eee eee eese see teens aane nasa aeterna rase tasa aas D 3 4 2 Connections in the Low Pressure Section eese eere tentant teen een acea soos D 3 4 3 Connections in the High Pressure Section henteeeesdenssseerereces MERE NR 3 4 4 Piston Seal Rear Flushing MEM NER MM 11 3 4 5 Purging the PUmmp csscesssccsessosesncetsnsccsenetssscensneesecteesssecdsssssescersneccesssssegecsteseaaee 11 3 5 Low Pressure Gradient System LP
59. removed U tube to the outlet valve Hand tighten and then tighten by a 14 turn with a spanner size 4mm Screw the suction tube to the inlet valve again Take care to avoid cross threading e To avoid contaminations entering the HPLC system the pump must be thoroughly rinsed prior to use at least 30ml HPLC water or purely organic solution Open the purge valve to prevent the rinsing liquid from entering the HPLC system Test the pump for tightness and if necessary tighten leaking connections 49 Gynkotek HPLC Operating instructions P 580 6 5 Pistons and Piston Seals Part No Description O10 TER f ae SEE 2 i d Fig 18a P 580 Working pump head Fig 18b P580 Equili bration pump 2 p head day s This informa e tion applies to 5 B en all P 580 mod m age els mi W To exchange the 10 No PartNo Description 11 122670032 Pistonunit_ amp n lt 11 j2 5001 0105 Piston retaining screw A 14 150252011 Piston seal retaining screw 12 J5 5025 2012 Pumpheadbushing Pun G 122060081 Ringsead o j 160252010 Supportringand I8 piston seal 8 9 50252006 U tube connection top 10 20540402 I Mdx4d0 Screw 1 111 50252005 Equilibration pump head piston seals perform the following steps described in the on Spray tube tomiing sec
60. rror levels The default settings should not be altered Tab Dialog Box Head Type amp Limits Use this tab to define the head types the pump type and the limits for the flow rate and the system pressure The following head types are supported Micro Preparative Analytical Micro Bio Preparative Bio and Analytical Bio Activating High Pressure Gradient is reserved for P 580 HPG models This setting allows selecting the double flow mode Gynkotek HPLC Operating Instructions P 580 standard micro semiprep Lower limit of the valid flow rate range _ Upper limit of the valid flow rate range Range Shows the currently valid pressure range of the system Minimum Lower limit of the valid pressure range Maximum i er limit of the valid Tab Dialog Box Relays Inputs The Relays Inputs tab lists available a and remote inputs OperableOut Relay 3 Relay Relay2 P580 MotorValve Motor Switch Valve Caution Ensure that the checkboxes corresponding to the relays remote inputs are activated Otherwise these inputs will not be available in CHROMELEON Tab Dialog Box Solvents The Solvents tab determines the number and names of the solvents connected to the pump Number of Solvents Number of connected solvents max 4 Solvent Names Enter the name of each connected solvent max 30 characters The n name will appear in the gradient display of an online con Finally save the changed server c
61. s on either side as shown in fig 14 To tighten use the supplied hex key size 4 Remove the handle assembly from the sample injector Fasten the sample injector from below to the mounting angle Ensure that the connecting ca ble points to the pump housing side Place the handle assembly on the mounting angle and tighten it with the hex key Fasten the capillary tube to the syringe loading sample injector and to the pump outlet Caution Use the Rheodyne fitting set to fasten the capillary tube to the sample injection valve see Fig 14 Connect the sample injector cable to the VO output of the pump Note It is also possible to mount the sample injection valve horizontally In this case use the two additional bore holes of the mounting angle When mounting the injector horizontally ensure that no air bubbles can reach the system via the syringe 17 Gynkotek HPLC Operating Instructions P 580 Caution Use syringes with the correct needle 0 028 x 2 sec Operating Instructions of the Syringe Loading Sample injector Caution If using a syringe loading sample injector from a different manufacturer use the ap propriate accessories For further information please contact the Gynkotek HPLC Service 18 Gynkotek APLC Operating instructions P 880 3 8 Installation under CHROMELEON 3 8 1 Required CHROMELEON Modules A prerequisite for controlling the HPLC pump P 580 with CHROMELEON is the corresponding CHROMELEON Control O
62. s well as three relay outputs The relay output Operable Out is closed as soon as the HPLC pump is not ready to operate i e in the event of an error or if the instrument is switched off Controlling the relays RI and R2 is via pump programming see section 4 4 6 or the data system CHROMELEQON or GynkoSoft in addition offer the possibility to control the relay Operable Out The functions of the digital inputs Start and Stop correspond to the same keys on the pump s keypad see section 4 1 The signal Hold interrupts the current gradient program and stops the flow Gynkotek HPLC Operating Instructions P 580 25 pln Connector Male plug solder side Ribbon cable pin assignment 1 9 Do not use 15121n0935 amp 6 amp 6 8528 8 6 0 10 OPERABLE OUT RELAY 3 OUT 1 P nm uL P LL IL LL P n n fs n C 25s 22f22 21 20 19 18 17 He isa C 1 OPERABLE OUT RELAY 3 OUT l LL n n n n nm n n n uL n D j 12 RELAY 1 OUT Y 13 RELAY 1 OUT o Qn T ee lu TT n o RET 14 RELAY 2 OUT 15 RELAY 2 OUT 16 17 Do not use 18 HOLD iN a marked wire 19 HOLD IN 20 STOP IN 21 STOP iN 24 22 20 18 16 14 12 109875654321 22 START IN 25 23 21 18 17 15 13 11 22 START IN KS 24 05 Donotuse Ribbon cable numbering Fig 5 25 pin VO Connector Caution Do not use the outputs 1 9 16 17 or 24 25 Note The relay outputs are zero potential switches Caution Maximum switching voltage of the relays is 24V 100mA must not be
63. sories filled with rinsing liquid You can also use a longer sili cone tube see fig 8 e Attach a piece of silicone tube supplied with ac cessories to the free pump head connector and place the free end in a glass or the solvent waste container e The pump must be rinsed at least once a day Es pecially when using saliferous solvents the dura bility of the piston seals can thus be considerably e increased Fig 8 Piston seal rear fiushing from above Caution The rinsing liquid must be miscible with the soivent otherwise the tightness of the pump may be impaired Caution The pump should not be operated for longer periods without rinsing as this may damage the piston seals and the piston 3 4 5 Purging the Pump 8 CESS Ones Outlet segment of the mix 3 chamber icone tube and qe i TPE Fig 9 Purging the pump view from right When purging the pump please note the following Attach a piece of silicone tube accessory to the outlet segment of the pressure sensor Fasten the other end of the tube to a plastic syringe accessory Set the flow to 1ml min while the flow is off LED Flow Off e Loosen the purge valve screw by one turn Open the corresponding dosing valve and use the syringe to draw in the solvent 11 Gynkotek HPLC Operating Instructions P 580 The dosing valves are opened as follows Ensure that the pump is disconnected from the CHROMELEON or GynkoSoft Data S
64. ssure spring VD 186 only up to S N 9805XXX i12116 0901 Tension spring 2146 1051 Spanner 1 4 x 5 16 2146 1052 Spanner 12 x 13 mm 2146 2603 Allen key 3 0 mm 2200 0011 10 Teflon solvent filters 2250 3011 Silicone tube 2 80 x 1 30 mm OD x ID 2261 0100 Fitting screw FS 7 2261 0101 Ferrule SR 7 12261 3190 FS 8 stainless steel 2266 0081 Ring seal DR 8 O ring 2266 0304 Piston seal 1 8 6025 2010 Kit P 580 piston seal PEEK support ring 2266 0311 Ring seal purge valve screw 12266 2021 Seal mixing chamber 2266 2022 Gasket pressure sensor 2267 0032 Piston unit 1 8 2281 2001 Plastic syringe 12 ml 2282 9185 Stainless steel tray 290 x 185 mm 5001 0105 1 8 Piston retaining screw 5001 0114 Slotted screwdriver for piston unit 1 8 5025 2006 U tube top stainless steel complete 3005 1800A U tube top stainless steel complete for double check valves only for preparative pump 5020 2100 Inlet double check valve complete 5020 2200 Outlet double check valve complete 5020 9110 Capillary connecting tube stainless steel 1 16 ID 0 25 mm m complete 5025 2021 Retaining screw f pressure sensor stainless steel 5025 1220 Coupling 5025 1780 Cover for pump block 5025 2001 Pump head left stainless steel 125025 2002 Capillary tube pump head left block mixing chamber 5025 2005 Pump head right stainless steel 15025 2010 Pump head bushing complete 50252011 Retaining screw piston seal pump hiead b
65. that the minimum pressure shut down is set This prevents damage resulting from leakage Furthermore dry running is prevented The pump must never be operated without rear flushing for a longer period of time see 3 4 4 otherwise the piston seals and the piston may be damaged For optimum compressibility compensation ensure to select the appropriate solvent types delivered by the pump prior to operating the P 580 Please follow the instruc tions in section 3 10 The pump must never run dry Damage to the pistons or the piston seals would result Buffers and solutions forming peroxides should be rinsed out after operation Before switching from buffer to organic solution the pump must be rinsed thoroughly with water When switching to another solvent ensure that the new solvent is miscible with the one contained in the pump Otherwise the pump can be damaged e g by floccula tions When interrupting operation for longer periods follow the notes in section 6 10 Jf the pump flow is interrupted for longer periods the lamps in any connected UV or RF detectors must be switched off to prevent evaporation in the flow cell 4 3 Choosing the Solvents Depending on the model wetted parts of the P 580 are manufactured from stainless steel PEEK sapphire ruby ceramics etc See also section 7 Use only standard HPLC solvents Note the special properties of the solvents e g viscosity boiling point UV absorption UV VIS detecto
66. the button again stops the flow LED Flow Off active the flow rate setting is kept e After pressing Flow you can use the arrow buttons T J to change the flow rate even during operation e For discrete input of the desired flow rate during operation you can use the numeric keypad However the entered value must be confirmed with J Enter Until the input is confirmed the LED Flow On will blink e Press the left Clear button to undo the last not yet confirmed input Note During operation the pump immediately changes the flow rate according to the en tered value 4 4 3 2 Mixing Ratio 0 1 to 100 adjustable in Steps of 0 1 e Use the buttons A B C D to display the mixing ratio of the individual channels on the Pres sure display The corresponding LED will be on It is not necessary to explicitly enter the portion of A as this portion is calculated from the difference of the other partial flows Use the arrow buttons Tand 4 to change the mixing ratio in single steps even during opera tion e Use the numeric keypad for the discrete input of the desired mixing ratio during operation However the entered value must be confirmed with J Enter e To use only one channel 100 mixing ratio simply press the corresponding button A B C D for approx 2sec The other channels are simultaneously set to 0 e Press the left Clear button to undo the last not yet confirmed input Caution To change the mixing ratio the por
67. the housing Fasten the top cover to the rear panel using the Knurled Screws e Reconnect the power cord Switch on the pump If the pump is controlled via data system reconnect the pump to CHROMELEON or GynkoSoft via the Connect command Warning The instrument may not be operated while the cover is removed Some components may carry current If you cannot find the cause of a leak please contact the Gynkotek HPLC Service 48 Gynkotek HPLC Operating Instructions P 580 6 4 Double Check Valve Replacement This information applies to all P 580 models On the working pump head of the P 580 there are two double check valves inlet valve and outlet valve The inlet valve is mounted to the pump head from below and the outlet valve from above When removed you can recognize the inlet valve by the inner thread which ends in a planar sur face while the outlet valve has a conical cavity for the capillary connection To remove one or both valves proceed as follows e Rinse toxic solutions from the pump as necessary Set pump flow to 0 Wait until the pressure in the system is reduced Unscrew the suction tube at the pump inlet and the U tube at the outlet Unscrew the corresponding valve cartridge with a spanner size 13mm Insert the new valve cartridge Ensure that you insert the cartridge in the correct solvent flow direction indicated by an arrow on the cartridge see fig 18a Tighten the valve Now attach the
68. tions below e Pump Head Removal Gynkotek HPLC Operating Instructions P 580 e Cleaning the Pistons e Piston Seal Removal e Piston Seal and Pump Head Replacement 6 5 1 Pump Head Removal e Draw out the stainless steel tray from under the housing as far as possible to catch any solvent that may be leaking If necessary rinse toxic solutions from the pump Set the pump flow to 0 Wait until the system pressure is down Remove all capillaries and connections from the pump heads Loosen the Allen screws of the two pump heads and carefully draw from the pump heads Carefully draw the seal rear flushing chamber including the seal see fig 18a gt from the pis ton t 9 9 9 6 5 2 Cloaning the Pistons You can reach the pistons via the pump heads from the front Opening the unit housing is not necessary Proceed as follows e Remove the pump head as described in section 6 5 1 e Clean the piston Note You can also remove the piston entirely to clean them Use the slotted screwdriver contained in the to undo the piston retaining screw see fig 18a b Caution For cleaning the piston use a household scouring cleanser only when you have com pletely removed the piston Otherwise the cleanser may stick to the piston and reach the system Do not use any waxy scouring cleanser e Apply some thin bodied resin free oil only to the metal part of the piston unit e After carefully rinsing the piston rub the piston several t
69. tions of B C and D must be entered explicitly If the total exceeds 100 the values are corrected as follows increasing the portion of B decreases the other portions in the following order A C and D Similarly in creasing the portion of C first decreases the portion of B and then D see section 4 5 for special notes on HPG models Note The pump immediately changes the mixing ratio during operation Note To display the current pressure again in the pressure display press the Max Min but ton until neither Max nor Min LED are lit 30 Gynkotek HPLC Operating instructions P 580 4 4 3 3 Pressure Limit 0 to 50MPa 500bar adjustable in Steps of 0 1MPa tbar Use the Max Min button to determine the maximum or minimum pressure limit Max LED Max is on If the operating pressure of the pump exceeds the limit shown in the Pressure display the pump flow is switched off Thus e g columns can be protected from ex cessive operating pressures e Min LED Min is on If the operating pressure of the pump is below this limit the pump flow is stopped This prevents dry running of the pump or continued delivery in the event of a leak e With the arrow buttons Tand J you can change the displayed pressure limit settings either LED Max or Min is active e Fora discrete input of the desired pressure limits you can also use the numeric keypad How ever the entered value must be confirmed with J Enter The respective LED Max or Min
70. tubes with filter frits Thus you will prevent contaminations from reaching the HPLC system Note Please check the suction frits for permeability Especially when working with aqueous solvents algae and other microorganisms can grow and be deposited on the filter frits Therefore use new solvents regularly Rinse the containers thoroughly before using them again Note For small flow rates the low flow connecting tube part no 5025 2515 can be used for connecting the left hand pump head and the 4 way valve Refer to section 6 9 for further details 3 4 2 Connections in the Low Pressure Section Solvent connections on the low pressure inlet side of the pump are realized with the following components 2261 1603 Supportflange No 2 2261 1602 Clampring amp 3 2261 0203 Knurnedfiting 2250 3151 PTFE tube PartNo Description Fig 6 Solvent connections on low pressure side Caution Do not overtighten fitting connections handtighten If necessary tighten leaking connections 3 4 3 Connections in the High Pressure Section On the model P 580 all capillary connections are supplied with fitting screws and ferrules Gynkotek HPLC Operating Instructions P 580 Caution Caution Caution Note Note No PatNo Descrip tion f1 2250 4011 Capillarytube f 2 2261 0101 ISR 7Ferule 3 2261 0100 Fig 7 Fitting screw and ferrule Do not overtighten fitting
71. upport ring part no 6025 2010 This is to prevent leakage e To remove the piston seal in the rear flushing unit you must first remove the retaining screw no 4 in the fig 8a b on the rear of the pump head bushing 6 5 4 Piston Seal and Pump Head Replacement Caution When installing the pump heads ensure that the open sides of both piston seal springs point away from the pump housing as shown in fig 18 Assemble the rear flushing chamber Insert the first piston seal into the pump head bushing for correct orientation see fig 18 Hand tighten the retaining screw e Slide the complete pump head bushing onto the piston unit into the pump block e Slide the ring seal the new support ring and the second piston seal onto the pump head bush ing Again ensure the correct orientation of the piston seal see fig 18 e Slide the pump head onto the pump head bushing until the second piston seai is correctly in place Please ensure the correct orientation of the working pump head left part of the pump block see fig 18 See the notes below Caution If you do not insert the piston seal in the way described above but into the disassem bled pump head it may get jammed and thus become useless Caution On the left side suction side the working head is installed It can be recognized by the two large bore holes for the inlet and outlet valves Ensure that the centered bore hole for the outlet valve as welt as the connecting pie
72. ure Remedy any leaks 47 Gynkotek HPLC Operating Instructions P 580 In the event that one of the built in leak sensors detects a leak the pump interrupts the current program and stops the flow the LED Flow Off is lit The LEDs A B C D MIN and MAX blink The following error message appears on the LCD display Internal Error Sensor detected leak CLEAR or FLOW Proceed as follows Press one of the Clear buttons on the keypad pressing Flow resumes operation Wait until the pressure is down Switch off the pump Disconnect the power cord Check whether soivent is leaking from the pump heads If this is the case tighten the tube and capillary connections Dry the components If necessary replace the piston seals e Draw out the stainless steel tray as far as possible from under the housing Dry the tray and the leak sensor attached to it e Replace the stainless steel tray e fno errors are reported after the pump is switched on again operation can be resumed e If the leak sensor still reports an error perform the following steps e Ensure that the power cord is disconnected Remove the cover from the P 580 e Loosen the inner retaining screws see fig 10 of the electronics main board lift and tilt the board to the outside e Check the capillary and tubing connections In the event of leaks tighten connections e Tilt the main board back into place and fasten it e Replace the top cover of
73. ushing 5025 2012 Pump head bushing M12 stainless steel 63 Gynkotek HPLC Operating instructions P 580 Purge valve screw complete Mixing motor complete Mixing chamber complete Tray for 4 way valve Suction tube P 580 D 1 5 mm Cap seal for purge valve screw Silicone wash tube 2 80x1 30 EDxiD 1 m Ceramic pressure sensor element Adhesive label 9mm white A Adhesive label 9mm white B Adhesive label 9mm white C Adhesive label 9mm white D Connecting tube degasser A 4 way valve A Connecting tube degasser B 4 way valve B Connecting tube degasser C 4 way valve C Connecting tube degasser D 4 way valve D Connecting tube rear panel A degasser A Connecting tube rear panei B degasser B Connecting tube rear panel C degasser C Connecting tube rear panel D degasser D Connecting tube 4 way valve pump head left ID 1 5 mm Connecting tube 4 way valve pump head left ID 1 0 mm Suction tube P 580 ID 1 5 mm 4 way valve Additional Spare Parts for Model P 580A HPG 2175 1011 Adhesive label 9mm white A 2175 1021 Adhesive label 9mm white B 2175 1031 Adhesive label 9mm white C 12175 104 Adhesive label 9mm white D 5020 9110 Capillary connecting tube stainless steel 1 16 ID 0 25 mm 1m complete 5025 2003 Capillary tube stainless steel complete for right pump head inixing chamber 150252516 Connecting tube left pump head valve right pump head valve
74. ution Programs are freely editable even during their execution However do not edit the currently performed program step This may lead to incorrect results Note Gradients are entered by defining the corner points Linear ramps are automatically executed on the basis of the set points Combined flow gradients are also sup ported Caution Bevor a previously created program Status set can be performed the program must be activated Status active Choose the program with the arrow keys then press the key 1 e The activated program can be started by pressing Start This is also possible while the status display is shown During the program run time the LED Flow On is lit e Press ESC to change to the Status display This allows you to view the entire current data of the active gradient program partial flows flow rate pressure program time program num ber e Press Stop to immediately stop a running program Delivery is continued with the current set tings and the program time stops in the Status display To stop delivery together with the program press Stop and then Flow so that the Flow Off LED is active The pump then stops Caution Before you can stop the pump flow Flow Off the current program must be termi nated by pressing the Stop button 35 Gynkotek HPLC Operating Instructions P 580 After completing a program press Esc to select a new program or to change to another item from the main menu 4
75. with the following European standards EN 50081 1 1992 Electromagnetic compatibility Generic emission standard Part 1 Residential commercial and light industry 4 EN 50082 1 1992 Electromagnetic compatibility Generic immunity standard Part 1 Residential commercial and light industry Caution Softron GmbH will not accept any liability for damage direct or indirect caused by connecting this instrument to devices which do not meet relevant safety standards Nov 26 1998 OM Manager Gynkotek HPLC Operating Instructions P 580 Table of Contents l Introduction P064 0444 99000004909 10409900004 REE l 1 About the Operating Instructions hund db b bh ee aa dd bb bh b deo b b o hohe HR ee m 1 2 Unpacking and Checking Contents esce ceeeeeecesee ER e 1 3 Warranty PTR RRC EEE LER EET TET TITEL LTPP LLL ETE EEL EEL LEE LEE ETE LEE e E 2 1 4 Intended Use cccccssssscsssascvcsecssossssscrevsossccussesserserseseenseseceeusetsceesssssessssssucecestsssesseverssesens d 3 3 2 Overview SERRA SSS ES SEES ee 2 J LTAL L L 2 1 Unit Description CIO he RR Rod E RERRER NR RE RE REGERE ee ee le Bel GEAR ERG Be Be Beet Bet alll tt ele elt Bel Bele ae ee 22 Principle of Operation SERM MN 2 3 Supported Configurations h b d d RR RR hn RE RR ee LL LLL ee Lee Le ELL Le ta 3 Installa tion did EEE ee ee ee ee ee 4 5 3 1 Positioning the Unit ee
76. ystems see sections 4 6 and 4 7 Please note that only one valve may be open at a time Reconnect the pump to CHROMELEON or GynkoSoft using the Connect command as necessary e When all tubes are sufficiently filled with the solvent you can press the Purge button on the front panel see also section 4 4 3 4 Close the purge valve screw again Ensure that the purge valve screw is not too tight 1t is bet ter to slightly tighten it later if there is leakage Caution Do not use any tools to tighten the purge valve screw If the purge valve screw is too tight the seal cap may be destroyed Caution The purge valve screw should only be opened or closed when the system pressure is down Goto the main menu see section 4 4 5 Now you can open the respective valves by pressing the buttons A to D gt A Valve A gt B Valve B Valve C gt D Valve D gt gt 12 Gynkotek HPLC Operating Instructions P 580 3 5 Low Pressure Gradient System LPG Warning Disconnect the power cord before opening the instrument Do not touch any elec tronic components On the degasser switching power supply board see fig 10 no 2 parts may carry current Caution Depending on the configuration equipment and position of the modules can differ from the illustration below ido Mbring chamber inlet cgiKnudedecrews EF Pump outlet SACD display ear LCD boa a 2 8 Purge valve sorew l t
77. zed for forming binary high pressure gradients The pumps deliver extraordinarily precise gradients The pump housing contains two complete pressure pump blocks If solvent supply is controlled via the optional solvent selector as shown below the pump can deliver two solvents on each pressure pump set in a high pressure gradient system Note The two pressure pump blocks can be rinsed independently Note The position of the switching or shut off valves and other modules may differ from the representation in fig 12 1 2 21 o 2b 20 3b 3g 3 4 19 3 18 6 17 B 9 16 10 Fig 12 open model P 580A HPG view from above lonr am Puge vave scerew ik pump moek tem 773 Selector valve pump biock 1 qon 8 Retaining screw pressure sensor A Cover camshat a Wave inet solvente 8 D ZR ubang chamber intet or 4E krurea sorewe Fa Valve outlet sovene 8 0 fpump block 1 et f2 Motor board Pump block righ Main board z Drive moor E F KOYDE SUR A Mixing chamber Inlet for pump bl 2 1 Retaining screw main board S drighty or with T plece blind plug i Equilibration pump head 25 E6 Pump ouet L 3 amp LCD dieplay rear LCD board c22 Powerswitch FJ Valve intet solvents A C 4 Keypad d board 15 Gynkotek HPLC Operating instructions P 580 3 6 1 Fluidic Connections of the High pressure Gradient System No Descripion 1 Rear panel w

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