BBD 6220 CO2 Incubator User Manual
Contents
1. Press any setpoint key Actual temperature CO O and rel humidity values are displayed again immediately 50 079 041 27 45 6 OPERATE Switching function Humidification ON OFF 28 43 Factory setting Humidification switched on set to 60 rel humidity Instruction Entry Keys Remarks Display Remarks Status Select function level 2 0 Hold down Indicator lamp control flashes and current function level is indi cated by the flashing display Zs be XP tee C Display circuit status Release and press factory setting 10 again c C Humidification ON C CO Change circuit status o Humidification OFF V Press C ENJE co Change circuit status o Humidification ON A Press C Hot co Exit function level 10 Press Actual temperature CO O and rel humidity values are displayed again after 1 minute Press any setpoint key Actual temperature CO O and rel humidity values are displayed again immediately 50 079 041 6 OPERATE Switching function Manual zero calibration of CO measuring system 75 Recommendation Check the CO concentration especially during long term tests F If there is a large deviation 0 5 between the displayed actual value for CO concentration and the nominal value the measuring system can be recalibrated du2. 5 C To avoid this open the doors for a short time This also applies to the reduced temperature If these measures are unsuccessful or if errors with undefined codes occur please notify our service department refer to Appendix When requesting our service please state the scanned error code 26 43 50 079 041 6 OPERATE Switching functions Press 0 Key page 19 17 to select the individual switching functions as required The indicator lamp control page 19 16 indicates when one of the switching functions specified below is selected 7 Acoustic alarm ON OFF 7 Humidification ON OFF 7 Manual zero calibration of CO measuring system m Setpoint lock in ON OFF m Door heater ON OFF Switching function acoustic alarm ON OFF Factory setting acoustic alarm switched on Instruction Entry Keys Remarks Display Remarks Status Select function level 1 m Press Indicator lamp control flashes and current function level is indi cated by a flashing display Af V Press mm c maaa Pand u Releaseandprossagan MEN C Acoustic alarm ON ENE co Change circuit status o acoustic alarm OFF ei Tess A C CO Change circuit status o acoustic alarm ON ANP es MO C FE co Exit function level zl Release Actual temperature CO O and rel humidity values are displayed again after 1 minute
3. m Reducing the germ count in the exhaust air by taking appropriate measures such as using high performance suspended matter filters Handling of human and animal pathogenic biological agents The following general rules also apply to the handling of human and animal pathogenic biological agents m Human pathogenic biological agents may only be handled with approval under the terms of the Federal Epidemics Act Approval under the terms of the Federal Animal Epidemics Act and the Ordinance concerning Agents of Animal Epidemics is required to handle agents of animal epidemics Pregnant women or nursing mothers are not permitted to handle infectious and human pathogenic biological agents or materials containing these agents Apply to cell cultures as appropriate Manufacturers certificates can be found in the memoranda of the Berufsgenossenschaft Chemie employer s liability insurance association for chemicals entitled Sichere Chemiearbeit Safe chemistry and the Berufsgenossenschaft f r Gesundheitsdienst and Wohlfahrtspflege employer s liability insurance association for health service and welfare They can also be obtained on request from the test centre of the expert committee on Health care and welfare Berufsgenossenschaft f r Gesundheitsdienst und Wohlfahrtspflege Pr fstelle des Fachauschusses Gesundheits dienst und Wohlfahrtspflege Pappelallee 35 37 22089 Hamburg Germany Source specification B003
4. C Adopt NEW temperature set Temperature inside unit is dis release point played Setting the CO setpoint Instruction Entry Keys Remarks Display Remarks Status Display CO setpoint co press Actual setpoint is displayed and P decimal point flashes Select CO setpoint CO amp A dr cken Setpoint increases co 8 dr cken Setpoint decreases Adopt NEW CO setpoint co CO concentration inside unit is release displayed Setting the O setpoint optional Instruction Entry Keys Remarks Display Remarks Status Display O setpoint 02 press Actual setpoint is displayed deci mal point flashes Select O setpoint 40 8 A press Setpoint increases 02 amp y press Setpoint decreases O concentration inside unit is Adopt NEW O setpoint 02 displayed release The control is deactivated when the unit is set for 21 O since this corresponds to the O content of air 20 43 50 079 041 6 OPERATE Setting the relative humidity setpoint Instruction Entry Keys Remarks Display Remarks Status Actual setpoint is displayed and Display the relative humidity rH press setpoint decimal point flashes Set the relative humidity set 4 4 2 A press Setpoint increases point aH amp
5. O m The sum of the nominal values for CO O must not exceed 90 Example 10 CO 80 O 90 possible 20 CO 80 O 100 impossible Before entering the chamber all gases pass through a filter where particles larger than 0 3 um are retained Filter efficiency is 99 998 A fan integrated in the rear of the interior wall ensures that the gases and the incubator atmos phere are thoroughly mixed The sensors for CO O option and relative humidity are also located on the rear panel of the in terior wall These sensors measure the parameters inside the unit and transmit corresponding sig nals to the closed loop controller A pressure compensation vent on the rear panel of unit avoids undesirable pressure build up in side the unit during the admission of gases he place of installation must be thoroughly ventilated in order to expel the gases escaping around pressure compensation vent 50 079 041 13 45 5 DESCRIPTION OF THE UNIT Humidification The atmosphere in the chamber is humidified through the admission of steam generated in a aktiv humifier Relative humidity rH can be regulated between 60 and 95 To ensure reliable operation of the steam generator only fill the reservoir with fully desali nated or distilled water The capacity of the reservoir is approx 4 7 Itr difference between min and max levels 2 2 ltr Door switch The heating system gas supply and humidifier are switched off when
6. e g to provide employees with instructions on accident prevention measures These sections are clearly marked in the list of contents Thermo Electron GmbH can accept no liability or responsibility for the marketability or the suitability of this instruction manual for a certain purpose other than that specified under Areas of application of equip ment Thermo Electron GmbH reserves the right to change the content of this instruction manual at any time and without prior notice As regards foreign language translations the German version of this instruction manual is binding This edition of the instruction manual applies to the BBD 6220 unit specified on the front page Date of publication 03 2006 Nominal charge Safety with regard to the protection of persons the environment and the material under process largely depends on the conduct of the persons using the equipment This instruction manual is important for your safety as well as for the setup installation use and maintenance of the equipment To avoid errors and any resulting damage especially personal injuries be sure to read this man ual carefully before using the equipment and follow all instructions 50 079 041 LIST OF CONTENTS PAGE Les GENERAL ca oe clare eects acct a AS ea aan eae daa ida 5 7 Explanation of icons in the instructions Manual 0 0 cece cece aee 5 Explanation of icons in the short instructions 0 ccc nc 6 General InSIUCUON
7. edition 1 92 ZH 1 343 of the Berufsgenossenshaft der chemischen Industrie employer s liability insurance as sociation for the chemicals industry Jedermann Verlag Dr Otto Pfeffer OHG PO box 103140 69021 Heidelberg 44 43 50 079 041 Thermo ELECTRON CORPORATION Internet www thermo com 50 079 041 45 45
8. is expected to provide anyone who works on or with the equipment with written instructions based on this instruction manual for the tasks to be performed These in structions should be easy to understand and in the language of the persons operating the equip ment This also applies to disinfecting and cleaning the unit also refer to Chapter 10 Maintenance 50 079 041 7 45 2 AREAS OF APPLICATION 8 43 The BBD 6220 gassed incubator is an item of laboratory equipment for cell and tissue cultures and can be used to simulate the physiological environmental conditions of cells The unit is generally suitable for setup and operation in the following areas n In laboratories for microbiological and biotechnological work m Medical and microbiological laboratories to DIN 58956 n Central laboratories in clinics and hospitals m Laboratories operating at safety levels L1 L2 and L3 Only organisms which comply with the requirements for safety levels L1 L2 and L3 may be handled in the unit The unit must be set up and operated in accordance with all applicable regulations in your country FRG ZH 1 119 ZH 1 342 ZH 1 343 ZH 1 598 It is not permitted to handle gases or agents whose vapours are combustible or can form a hazardous potentially explosive atmosphere when mixed with air Be sure to comply with the applicable regulations in your country FRG ZH 1 10 50 079 041 3 SAFETY INSTRUCTIONS In the case of biolo
9. tention must be paid to the information given in the instruction manual or accompany ing documents Water supply vent overflow Add drain water quick release coupling Marks information in the instruction manual for optimizing use of the equipment aqua dest ICON CN AS za des start Disinfection mode key operated switch stop BN Marks information for higher surface temperature in the disinfection mode Overtemperature protection temperature limit controller Selection of switching interlocking functions fi Error code enquiry acknowledgment of acoustic signal Change switching functions activated display Automatic startup of unit and calibration of measuring systems auto zero calibration active display des Disinfection mode display Information on proper disposal recovery of raw materials Sai 50 079 041 5 45 1 GENERAL EXPLANATION OF ICONS TO THE SHORT INSTRUCTIONS Switch on unit operate the main switch aqua dest Check the water level min aa o Set the temperature limit controller 7 Close unit doors Read the operating instructions Y N auto start pec San La r stop Start disinfection routine gt 1sec auto start ne automatical auto st
10. 7 press Setpoint decreases Adopt NEW relative humidity Relative humidity concentration erH release E ear setpoint inside the unit is displayed Disinfection routine The entire interior of the unit together with all sensors can be disinfected with hot air at 180 C The disinfection routine lasts approx 13 hours Start disinfection routine Instruction Entry Keys Remarks Display Remarks Status Open the unit door for at least All displays flash 30 seconds Remove samples and vessels from the interior of the unit Activate the disinfection rou Mee Indicator Lamp page 19 11 tine turn key in direction of stop flashes and indicator lamp page 15 arrow 2 comes on Press for approx 1 second Close the door Disinfection routine runs automati cally Temperature Limit Controller is dis abled CO display indicates the phases of the disinfection routine auto start routine begins on com pletion of the disinfection routine In the disinfektion mode appr 13 h occur slightly higher temperatures on the outer sur I face the door area and the internal door grip When LED des is flashing proceed with particular caution Avoid touching the door areas during the disinfection routine 50 079 041 21 45 6 OPERATE Disinfection phase Temp Heating phase Cooling phase Flow chart of d
11. APPENDIX The unit is not suitable for stacking when operated in the manner intended 50 079 041 4 SETUP AND INSTALLATION Clearances When setting up the unit the minimum clearances between the unit and adjacent surfaces and other units must be maintained Larger clearances are recommended to facilitate accessibility for installation and the supply con nections pra 100 3 4505 100 gt on e LH Y l Y o Fig 1 Minimum clearances in mm Supply connections l Mains socket o CO gas connection _ 0 N gas connection option Label supply connection Potential free contact RS 232 interface Fig 2 Supply connections rear panel of unit Mains connection Prior to connecting the unit to the mains make sure that the power supply ratings match those stated on the nameplate Your unit is equipped with a power supply cable with a grounding contact type plug Fuse protection required 7 Use a type G 16 circuit break switch or a type T 16 A fusible cutout Refer to the Chapter titled TECHNICAL SPECIFICATIONS for unit power consumption 50 079 041 11 45 4 SETUP AND INSTALLATION Gas supply connection The gas connection are located on the back of the unit The gas must be supplied to each unit via a pressure reducer with the inlet pressur
12. are decontaminated through hot air disinfection at 180 C The sensors can be left inside the unit during the disinfection process Do not spray the O rel humidity sensors porous sintered material with disinfectant Recommended disinfectant A surface disinfectant recommended by Thermo Electron Corporation can be ordered under following part numbers m Spray bottle 250 ml Part No 50052425 7 Refill bottle 500 ml Part No 50051939 NOTE Decription disingectant Details for efficiency and approvals are available on request Order Disinfection 7 Remove samples cultures etc from the unit m Switch off the unit m Remove shelves and carrier racks Remove safty device from the rear panel of the inner vessel with tools e g screw driver and detach them by lifting it up Clean inner housing shelves and carrier racks using a warm skin sensitive detergent Pril and allow them to dry completely Do not wet sensors 4 6 8 with liquids and disinfectants 1 Lead through and pressure compensation vent 2 Steam outlet Do not fill unit with any liquids only clean the surface Recirculated air blower Pt 100 temperature sensors Relative humidity sensor Fig 8 Rear wall of inner vessel Also clean glass door and seal with detergent and allow them to dry completely m Then spray surfaces and seals with surface disinfectant and allow it to react in accordance with th
13. glass screen right 50 030 022 Seal for glass door for gas tight glass screen 50 041 536 Filler canister 50 029 126 Equipment fuse T 6 3 A slow blow 2 pcs 03 002 641 Sealing plug for feed through without hole 03 669 008 Sealing plug for feed through with hole 50 029 827 Subframe 300 mm high 50 031 348 Subframe 780 mm high 50 029 597 Pressure reducer for CO 03 429 937 Pressure reducer for N 03 429 942 Pressure reducer for O 03 429 943 50 079 041 37 45 12 TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS UNITS VALUE MECHANICAL Outer dimensions W x H x D mm 920 x 855 x 775 Inner dimensions W x H x D mm 607 x 669 x 585 Chamber volume l approx 220 Divided Shelves W x D mm 260 x 500 Quantity included in scope of supply pcs 3 Max quantity pcs 12 Max surface load kg 10 per shelf Max gross load of unit kg 30 Weight of unit excluding accessories kg 107 net THERMAL Ambient temperature range C 18 30 Temperature CO equipment C Ambient temp 5 50 control range CO O equipment C 7 50 Temperature deviation temporal DIN 12 880 Part 2 K lt 0 1 Temperature deviation at 37 C K lt 0 5 spatial DIN 12 880 Part 2 50 C K lt 0 7 Heating up time with auto start ambient temperature 22 C unit empty to 37 C h approx 5 Cooling time ambient temperature 22 C unit empty from 37 C to 25 h approx 5 Heat release into s
14. outside door 22 2 eee eee rr 14 Disinfection TOUTING asics eo a een Be an ate eee el de eae eee ba ee ee nee 14 Potential free contact connection 6 cece nc 14 67 ORERATE A e IER INNS tte ada a a ee e 15 30 SWICK Panel sre A A ro E AAEE ENEA 15 Weater Supply escindida E Ad RES 16 MaInS SWICh 2 A A tape A O AS A A 16 Temperature protection device temperature limit controller 020 cece cece eee ee o 18 Control and display panels 1 ccc cnn 18 21 Disinfection routine serorea nerea i e te tenet e need ee ee ER Na eaa eera 21 22 auto start routine auto zero routine 1 e teen nents 23 24 Errorcode enquiry ceci a a a iia SPM A a te eens 23 25 Switching TUNCHONS riada bade Oh tee tad Gerda ree ee ceeds oth dd 27 Switching function Acoustic alarm ON OFF 0 0 0 ccc cece e eee carr 27 Switching function Humidification ON OFF 0 c cece cence eee eee eee eee eee eens 28 Switching function Manual zero calibration of CO measuring system 0 cece eee eee 29 Switching function Setpoint lock in ON OFF 2 2 cee carr 30 Switching function Door heater ON OFF 0 0 ccc eee teen nee een e teen eee nne es 31 50 079 041 3 45 LIST OF CONTENTS PAGE 10 11 12 13 14 15 4 43 STARTUP DW A nets Gece Cha A a a EEA 32 OPERATION suas o 33 Guidelines for USE visitar acts ira 33 Before putting the unit into operation oococcccccccccc
15. 50 079 041 5 DESCRIPTION OF THE UNIT Levelling the shelves aaa y The shelves can be aligned with the aid of a spirit level with height adjustable feet Functional principle The atmosphere in the chamber can be regulated in relation to its temperature CO concentra tion O concentration optional and relative humidity Heating system The temperature inside the unit can be regulated in the range of 7 C to 50 C but must be at least 5 C approx 7 C for the O version above the ambient temperature of the unit Condensation on the glass door is largely prevented by heating the unit door If the unit door is left open for a long period of time however condensation cannot be ruled out Qa The unit door heater can be switched off as required refer to Chapter headed OPERATION This enables the unit to be operated at high ambient temperatures or when the operating tem perature is only supposed to exceed the ambient temperature by approx 4 C Example Ambient temperature Operating temp Heating of unit door 25 37 C ON 24 28 OFF 32 to 33 37 OFF Gassing Connection to the gas supplies CO O or rather N are located on the rear panel of the incuba tor 7 The CO content of the atmosphere in the chamber can be regulated in the range 0 to 20 m The O concentration inside the unit can be regulated in the range 3 to 90 O by admit ting N lt 21 O or O gt 21
16. ACTICE 50 079 041 9 45 4 SETUP AND INSTALLATION Transport When setting up installing and operating the unit be sure to comply with all applicable regulations in your country FRG ZH 1 119 ZH 1 342 ZH 1 343 ZH 1 598 Handle the unit with care during transport Do not lift up the unit by its door Refer to TECHNICAL SPECIFICATIONS for weights and dimensions Place of installation 3 Stacking 10 43 The place of installation must be dry and draught free The unit should not be placed or operated in recesses which cannot be ventilated To avoid or at least minimize deviations from the technical specifications the tempera ture at the place of installation must be in the range 18 C to 30 C Set up the unit on a solid non flammable surface laboratory tables subframes Make sure that the unit is in a stable and perfectly upright position Do not cover or obstruct the fresh air or exhaust vents in the unit housing please observe the minimum clearances see page 11 CO are admitted into the gassed incubator CO is a health risk hazard The place of installa tion must therefore be well ventilated Gases discharged from the pressure relief valve must be safely expelled outside by means of the ventilation systems It may be necessary to take special measures in order to ensure proper ventilation when several units are installed in the same room For information on gas flow during operation refer to the
17. CULTURE MEDIA CO2 42 43 pH value of culture media Dependency on the CO2 concentration Media Na HCOs g l DMEM 3 70 BME 2 20 MEM 2 20 J Medium 199 2 20 Mc Coy 2 20 F 10 1 20 F12 1 20 MEM 0 85 Media with Hanks Salts 50 079 041 BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE General rules Keep windows and doors in the working areas closed while work is in progress Do not consume drinks food or smoke in the working areas Do not keep foodstuffs in the working areas Lab coats or other protective garments must be worn in the working area Oral pipetting is prohibited Use pipetting aids Only use needles and syringes if absolutely necessary With all manipulators be sure to avoid aerosol formation as far as possible On completion of work and before leaving the working area clean hands thoroughly Disinfect and remois turize hands if necessary Keep working areas clean and tidy Only equipment and materials actually required should be on the work tops Keep stocks in the areas or cabinets provided for them only Regularly check the identity of the biological agents used if this is a requirement for hazard potential assessment The length of the interval between checks should be set according to hazard potential With regard to the handling of biological agents employees must be issued with verbal instructions relating
18. Circuit break switch G16 Rated power consumption during kW 1 2 1 2 incubation disinfection MISCELLANEOUS Sound pressure level DIN 45635 Part 1 dB A lt 50 max r H of the ambiance r H 180 Height mNN 2 000 Under some circumstances eg when working with culture vessels without covers the control range will be restricted to 90 95 since below this level evaporation of the medium will lead to an increase of the relative humidity in the chamber 50 079 041 39 45 13 MATERIALS USED DISPOSAL 40 43 Components Material Outer housing Inner vessel shelves Glass pane Door seal of unit Glass door seal Thermal insulation Control and display membrane Heating systems Leads Other components Galvanized sheet steel painted to RAL 9002 Stainless steel material No 1 4301 Sodium silicate glass Magnetic core sheathed in soft PVC Tempered silicon PU foam sheet CFC incorporating non woven needled glass fibre quilt binder free Polyethylene Silicon sheathed resistance heating conductor and tubu lar heater Plastic sheathed copper strands Sheathed electrical components coated in various plas tics partly mounted on printed circuit boards bonded with epoxy resin Disposal WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo El
19. Operating instructions Hot Air Disinfectable Gassed Incubator BBD 6220 AN Read this operating instructions and keep it near the equipment for reference purposes Valid 03 2006 50 079 041 A Thermo ELECTRON CORPORATION 2 43 Postal adress Germany Thermo Electron LED GmbH Robert Bosch StraBe 1 D 63505 Langenselbold Enquiries from Germany Phone Sale 0800 1 536376 Service 0800 1 112110 Fax Sale Service Postal address USA Thermo Electron Corporation 275 Aiken Road Asheville NC 28804 USA Enquiries from North America Phone 1 800 879 7767 Fax 1 828 658 0363 E Mail info labequipment de thermo com 0800 1 112114 E Mail Enquiries from Latin America info labequipment de thermo com Phone 1 828 658 2711 Fax 1 828 645 9466 Enquiries from Europe Middle East and Africa E Mail Tel 49 0 6184 90 6940 iinfo labequipment de thermo com Fax 49 0 6184 90 7474 E Mail Enquiries from Asia Pacific info labequipment de thermo com Phone 852 2711 3910 Fax 852 2711 3858 E Mail info labequipment de thermo com Copyright Thermo Electron GmbH D 63505 Langenselbold Deutschland No part of this publication may be reproduced or transmitted in any form or by any means including pho tocopying and recording without the written permission of Thermo Electron GmbH Various sections of this instruction manual may be copied only for in house use by the equipment opera tor
20. S sated tbc cfs ON k ARRAES era la GATE aed RACE Ine dow beste cane Lede 7 Equipment log DOOK sica ts Datei teed nied eam dtl tender A A coats 7 Operating NSiUUCtONS cocina rada paedavte donate da 7 2 AREAS OF APPLICATION iaa vee Sen re ren tee eee eee ee ee eee Vea ee 8 3 SAFETY INSTRUCTIONS oo icicd ecient eck we Se we eee ce tae eee A ive aid wee ee 9 4 SETUP AND INSTALLATION 00 00 eines vise eign wesee tee She ee ati ete eee eee ee 10 12 TRANS POM 3 chines ada rana patas 10 Place of installation cicecvel oia logan dada samades dada 10 STACK it ete case eames AA ete qc vane ao dai pal 10 Clear aces A Ls ea teed a A a ern A eed heehee hate 11 SUPPIY CONNECHONS tree scroniedenetshadadianee soe Macs rail arts dare ias 11 Mains CONNECH N sese desd bodes tee nda asas ariba 11 Gas SUppIY CONNECUON sasiet anarie AS ARA AA 12 RS 232 IMENICE 0 A A A AA AA a 12 Potential free contact connection 0 cece cee eee eee rr een ete t bene nenneee 12 5 DESCRIPTION OF THE UNIT occ cee bee ated ee eee ee elec bee wee ee Da eee ee 13 14 Levelling the shelves ui A a a 13 Functional PrINCIP S cera da oh ee eee a Gad ee ee ee 13 heating SYSTEM tc seas ss Aa eg vate Beate ca ete Sle Gat ae ed o oe 13 GASSIING A Ai aA a Beale teas Ae 2 fa 13 H miditicaton Li A A te ee ae Ada a AAA thle AA 14 Door switch nd peas 14 ernal MIMOS A A AA A AA A ENA 14 Six piece Gas tight glass screen wk cee eee ee ened eden e een e ee enaes 14 Lockable
21. again immediately Actual temperature CO O and rel humidity values are displayed 50 079 041 31 45 7 START UP A 32 43 The items specified in brackets refer to Fig 4 on page 15 Instruction Entry Keys Remarks Display Remarks Status Open unit doors Remove transit restraints and accessories from inside the unit Carry out basic cleaning Water reservoir Fill unit with water Observe max level use destilled or fully desalinated water Switch on unit Mains switch 8 1 All display elements come on for approx 15 seconds auto test of controller O version The O measuring system is started up automatically on completion of the auto test Duration approx 5 min O display reads run Adjust setpoints Control and display panel A Refer to page 18 21 Set temperature protection de vice Adjust Temperature Limit Control Refer to page 18 ler 6 Select switching function s as required On control and display panel refer to page 20 and 27 to 31 Activate auto start Indicator lamp auto start Ale V Press flashes Hold down for approx 5 seconds Close all unit doors Temperature displays actual value CO displays 0 0 O displays 21 0 rel humidity displays actual value auto start routine runs automatically m Unit is set to entered temperature and relative humidity set poi
22. art Open unit doors for at least 30 seconds v 30s 310 e 4 it gt CO y v gt 210 xo B E Dd Set the desired setpoints 950 AH T auto start X f gt 5s amp activate the auto start start stop 7 O End of the auto start bring in the samples Take out the samples open unit doors for at least 30 seconds Sao MaS Flow chart of disinfection routine 6 43 50 079 041 1 GENERAL General instructions e To avoid errors and causing damage especially personal injuries be sure to read this manual carefully before using the equipment and follow all instructions When setting up and operating the unit be sure to comply with the instructions contained in this manual as well as all applicable laws regulations and directives in your country FRG ZH 1 119 ZH 1 342 ZH 1 343 ZH 1 598 The unit meets the following safety requirements m DIN 12880 Part 1 11 78 m DIN VDE 0700 Part 1 IEC 335 1 EN 60335 1 11 90 7 EN 61010 When you have an enquiry order replacement parts or file a complaint please state the data on the nameplate and if applicable the fault code Equipment log book We advise you to keep an equipment log book Keep a record of inspections and testing calibration work and any major work carried out on the unit such as maintenance agents loaded etc in this log book refer to APPENDIX Operating instructions The operator employer
23. ated and the O sensor optional is calibrated for 21 7 Indicator lamp page 18 17 auto start goes out 7 The auto zero routine determines the reference value and O sensor optional is calibrated n Gas is admitted until the CO O setpoints are reached The auto zero routine compensates for zero drift of the CO measuring cell The auto zero routine runs automatically every 6 hours When the indicator lamp page 19 13 auto zero is flashing the auto zero routine is in progress If the door is opened or closed during the auto zero routine auto zero calibration is restarted If the auto start has not been completed after 24 hours at the latest page 19 indicator lamp 17 auto start is still flashing the auto start will have to be cancelled manually refer to page 24 and repeat 50 079 041 23 45 6 OPERATE Instruction Entry Keys Remarks Display Remarks Status Cancel auto start routine Ala lv Indicator lamp page 19 17 auto start goes out and auto zero cali Press for approx 5 seconds bration routine starts Indicator lamp page 19 13 auto zero flashes Cancel auto zero routine Ala y Indicator lamp page 19 13 auto zero goes out Press for approx 5 seconds Unit displays the current actual values If a repeat auto start auto zero routine is unsuccessful contact the service department of Kendro Laboratory Products E
24. ccncn cnn cnc 33 DUI OPEN ii EA ede a A A A a 33 Operation interruptions 0 ccc cece eee eee eee eee eee eee teen ee eae 33 SHUTDOWN 2200 A cette A AA AAA haere te 34 MAINTENANCE ii a A Al 35 36 Cleaning Disinfection 00 ccc cece nc 35 TOSUNG id a IR 36 Equipment og DOOK isdee enhance AA 36 Replacing of electrical parts 0 cece nr rr en ene e ees 36 AUTHORIZED EQUIPMENT PARTS AND ACCESSORIES 2000ce cece eee e eee eee eeeee 37 TECHNICAL SPECIFICATIONS 0c0ococcccc eee eee eens 38 39 MATERIALS USED DISPOSAL dic tint tie ee ene a 40 GAS CONSUMPTION ud A le le et ee ee ee eee 41 PH VALUE OF CULTURE MEDIA 0 ccc cess cette eee eee eee eee 42 Basic rules of sound microbiological engineering practice 00 ssee cece cece eee 43 44 Geoneralrules 2 53200 pein e pee eet eee naa abra 43 Handling of pathogenes 0 cece eee eee eee eee eee etnies 43 44 Handling of human and animal pathogenic biological agents 06 cee eect nents 44 APPENDIX Statement of conformity Log Book Explanation of confirmation of safety Information request form regarding maintenance and servicing contract Communication protocol of RS 232 device 50 079 041 1 GENERAL EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL In chapters of the instruction manual which deal with safety this icon appears under the title of the chapter Displayed on the equipment this icon denotes that special at
25. e manufacturer s instructions for use m Then rinse surfaces with distilled water several times until all traces of disinfectant have been removed Collect the washings The surfaces of the unit can also be re rinsed or sprayed with a 70 by vol alcohol solution analytically pure isopropanol if required 50 079 041 35 45 10 MAINTENANCE A Attention Be sure to comply with the safety instructions and applicable regulations in your coun try Alcohols or solutions with an alcohol content of greater than 10 can ignite or form explosive gaseous mixtures when they come into contact with air and should therefore only be used in well ventilated places Do not expose to naked flame Equipment and component parts cleaned using alcohol or alcoholic solutions must not be ex posed to naked flames or other possible hazard sources Wait until dry before use 7 Install the parts in the reverse order of removal as appropriate 7 Disinfect the unit with hot air at 180 C disinfection routine m After disinfection put the unit back into operation as described and always check that it is safe to operate prior to loading Testing The serviceability and safety of the unit are only guaranteed if the necessary testing maintenance and repair work is carried out by the Thermo Electron Gmbh department or by staff authorized by us The unit should be checked for safety leakproofing and serviceability at least once a year Equipment log b
26. e set to between 0 8 and max 1 bar This setting must not be changed for safety reasons FRG ZH 1 119 E gt The gases must have at least 99 5 purity If several units are placed in the same room special ventilation measures are required n CO gassing Connect the gas cylinder to the gas nozzle on the unit 7 O N gassing optional If you intend to run the unit with an oxygen content above 21 connect an oxygen cylin der If you intend to run the unit with an oxygen content less than 21 connect a nitrogen cylin der To deactivate the oxygen control set the nominal value to 21 0 average oxygen content of ambient atmosphere RS 232 interface Serial interface for computer aided acquisition and documentation of critical operating parameters temperature CO relative humidity error codes etc The RS 232 interface can connect a single unit in combination with the Kelvilog software package and can be expanded to connect 31 units with the Netcontrol hard and software package Potential free contact connection The Potential free contact 1 changeover contact is rated for the following circuits Circuit Voltage Fuse to be installed by customer Mains operated circuits max 250 V AC max 6A SELV SELV E circuits 25 50 V AC max 2 1 A cf VDE 0100 Part 410 60 120 VDC max 1 0 5A Fig 3 Application example of Potential free contact connection 12 43
27. ectron Corporation has contracted with one or more recycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Thermo Electron s compliance with these Directives the recyclers in your country and information on Thermo Electron products wich may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS 50 079 041 14 GAS CONSUMPTION Gas consumption during operation Gas consumption corresponds to the gas quantity re of BBD 6220 gassed incubator leased at the back of the unit which has to be discharged safely into the outside air by ventilating the placement area Gasing with CO2 CO2 concentration Time min Amt of gas supplied 8 Ih Gas inlet pressure 1 bar Gasing with Oxygen 02 Nitroge N2 O2 concentration O2 0 20 40 60 80 100 Time min Amt of gas supplied O2 9 8 l min N2 7 4 l min Gas inlet pressure 1 bar Gas consumation CO2 0 9 e e N 2 o CO2 consumtion l h 2 w 0 2 0 1 0 2 4 6 8 10 12 144 16 18 20 CO2 concentration CO2 Amt of gas supplied 8 h Gas inlet pressure 1 bar Gas consumtion of N2 02 o N gt 02 consumtion l h 0 10 20 30 40 50 60 70 80 90 100 O2 concentration O2 Amt of gas supplied O2 9 8 l min N2 7 4 min Gas inlet pressure 1 bar 50 079 041 41 45 PH VALUE OF
28. et to approx 2 C above the nominal temperature set at the temperature controller it assumes the function of load protection protection of unit its environment and loaded material The load protection function is only effective above room temperature If Fault is displayed during operation m Check the settings on the Temperature Limit Controller and the controller and correct them if necessary If the fault cannot be rectified contact our service department display panel A Microprocessor controlled controller with digital temperature CO O optional and relative hu midity displays The controller has the following functions m Temperature set display and control 7 CO concentration set display and control n O concentration set display and control OPTIONAL 7 Relative humidity set display and control m Error code scan m auto start function activate n Switching functions Acoustic alarm switch ON OFF Humidification switch ON OFF Manual calibration of CO measuring system execute Setpoint lock in select ON OFF Door heater switch ON OFF 50 079 041 6 OPERATE 1 2 3 4 5 6 7 8 Fig 7 Control and display panel A 1 Indicator lamp 2 Display 3 Indicator lamp 4 Display 5 Indicator lamp 6 Display 7 Indicator lamp 8 Display 9 Key 10 Key HEATING Temperature CO GASSING CO concentra
29. gical incubators biological safety with regard to the protection of persons its surroundings and the load is heavily dependent on the observance of the applicable regulations by the persons using the equipment Even then however the possibility of hazards especially health hazards arising cannot be ruled out The residual risk depends on the work performed in each individual case To avoid errors and causing damage especially personal injuries be sure to carefully read this manual before putting the equipment into operation and follow all instructions Safe and reliable operation of the unit can only be guaranteed if the necessary inspections maintenance and repair work are carried out by Heraeus Service Department personnel or by personnel authorized by our company Gas is to be supplied to each unit by means of a pressure reducer with the inlet pressure set to between 0 8 and max 1 bar This setting must not be changed for safety reasons FRG ZH 1 119 The place of installation must be thoroughly ventilated in order to expel the gases escaping around the pressure relief valve to the outside To maintain the temperature protection function the functioning of the overtemperature protection device temperatur limit controller must be checked at reasonable intervals Only original replacement parts authorized by the manufacturer are to be used Refer to the Appendix titled BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEER ING PR
30. ice trips or an error is detected by the diagnosis system a fault message is sent to the customer s own signalling system 14 43 50 079 041 6 OPERATE Switch panel 6 j A Control and display panel refer to Fig 7 1 Indicator lamp Fault overtemp protection A 2 Indicator lamp Disinfection mode leraeus x 3 Indicator lamp Master Switch ni e ES 4 Vent overflow Water supply i festa 5 Level gauge Water supply a re 02 6 Setting knob Temperature protection device aosan 7 Press button Disinfection mode A i i al 8 Master switch z 0 i 9 Quick release coupling Add drain water 1 2 e eT 3 m 8 4 m O Fig 4 Switch panel 50 079 041 15 45 6 OPERATE Water supply Level gauge 5 m indicates the water level 7 The water level should be in the range between min and max This range is equivalent to a volume of 2 2 ltr If the water supply falls below the min level a reserve of 0 5 ltr is still available Filling the water reservoir 16 43 For humidification of the incubator water of the following quality must be used m demineralised water that has been sterilised or sterile water that has been demineralised Distilled or autoclaved water is suitable Fill the canister with the water and leave the scre
31. iseases As generally recognized rules of indus trial medicine they enable the doctor to assess evaluate and record the results of examinations according to the same criteria Apply to cell cultures as appropriate Source specification B003 edition 1 92 ZH 1 343 of the Berufsgenossenshaft der chemischen Industrie employer s liability insurance as sociation for the chemicals industry Jedermann Verlag Dr Otto Pfeffer OHG PO box 103140 69021 Heidelberg 50 079 041 43 45 BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE Potentially hazardous genetically engineered organisms viruses and subviral agents must be handled in accordance with employers liability insurance association guideline G 43 entitled Biotechnology m Instructions for administering first aid in the event of accidents involving pathogenic microorganisms and viruses must be immediately to hand in the working area The person in charge must be notified of all accidents immediately Depending on hazard potential it may be necessary to take further safety precautions Use of class I class II type approved or class III safety workbenches air flow pointing away from experimenter Restricting and supervising access to specific areas Use of special protective garments and breathing apparatus Disinfection of all materials containing pathogenes before they leave the worktop Maintaining a partial vacuum in the working area
32. isinfection routine Status display on CO display Heat The chamber is heated to 180 C the disinfection temperature can be read off the temperature display m Disinfect When the disinfection temperature of 180 C is reached a disinfection phase with a duration of approx 3 hours is started If the door is opened during the period and the temperature drops below 180 C the disinfection routine restarts automatically E Cool On completion of the disinfection phase the unit cools down to the originally set nominal temperature m The yellow indicator lamp des as soon as the unit reaches the set operating temperature e g 37 0 C is reached The sensors are automatically calibrated auto start and the unit is readjusted to the set gas rel humidity setpoints The disinfection routine is completed m If a temperature of 200 C is exceeded during the disinfection routine the latter is cancelled and all poles of the heating system are disconnected Error message 501 is displayed Cancelling the disinfection routine Instruction Entry Keys Remarks Display Remarks Status Cancel disinfection des The CO display indicates the cool stop ing phase of the disinfection routine Press for approx 1 second 22 43 50 079 041 6 OPERATE auto start routine auto zero routine An auto start routine auto zero routi
33. ller A temperature protection device is built into the unit and acts as a temperature limit controller It conforms to Thermal Safety Class 3 1 to DIN 12880 Part 1 11 78 In a functional state the temperature limit controller assumes the control function if the set temperature is exceeded The red indicator lamp 1 Fault lights up as soon as the temperature limit controller responds Fig 6 Temperature The cutout temperature of the temperature limit controller can be adjusted in the range limit controller 6 AN Control and 18 43 20 to 55 C using a coin or screwdriver Have your temperature protection device tested for proper operation at reasonable inter vals This applies in particular before prolonged work processes Operational test Condition Nominal temperature controller reached constant To run this test turn the dial on the Temperature Limit Controller so that it shows approx 5 less than the temperature indicated on the temperature controller If the Temperature Limit Controller responds and the red indicator lamp 1 Fault comes on the Temperature Limit Controller is operating properly Now set the Temperature Limit Controller to the required value depending on the max cutout temperature m If the Temperature Limit Controller is set to the upper temperature limit it assumes the function of unit protection protection of unit and surroundings m If the Temperature Limit Controller is s
34. ne will have to be run when putting the unit into operation or if the temperature setpoint is altered by more than 1 C Wr To ensure that there is only air i e no CO in the chamber before the auto start routine is acti vated ventilate the chamber by opening all doors The auto start can only be activated if the doors are open for at least 30 s The indicator lamp auto start page 19 17 indicates when the auto start routine is running Calibration can take up to 5 hours especially if the unit is cold If the unit door is opened during the auto start the auto start routine auto zero routine restarts automatically after the unit door is closed On resumption of the power supply after a power failure the auto start routine auto zero routine is also restarted Instruction Entry Keys Remarks Display Remarks Status Open unit doors for at least All display segments flash 30 seconds Adjust or check the set Refer to pages 20 points Activate auto start Indicator lamp page 19 17 auto A amp V Ww start flashes Press for approx 5 seconds Close all unit doors Temperature reads Actual value CO reads 0 0 O reads 21 0 optional rel humidity reads Actual value The unit is set to the temperature and relative humidity setpoints When temperature and relative hu midity setpoints are achieved consistently the CO measuring system is automatically calibr
35. nts Relative humidity is built up m When a constant temperature and relative humidity are reached the CO and O measuring system is calibrated automatically m Indicator lamp auto start goes out auto zero routine for determining reference values is exe cuted m Gas is admitted until the entered CO O setpoints are reached 50 079 041 8 OPERATION Operating control The microbiological safety of the unit is largely dependent on the proper conduct of the persons using the unit Refer to the Appendix Basic rules of sound microbiological engineering practice Be sure to comply with the instructions contained in this manual as well as all applicable regula tions and directives in your country FRG ZH 1 119 ZH 1 342 ZH 1 343 ZH 1 598 Before putting the unit into operation m Put on all safety garments necessary to protect hands face and body remove jewelry 7 Disinfect and clean the chamber and fittings regularly Only use lint free materials to wipe surfaces clean m Do not use explosive disinfectants When using alcoholic disinfectants be sure they comply with all applicable regulations of your country FRG ZH 1 598 During operation Correct loading determines the temperature conditions in the chamber If large objects or auxiliaries are placed inside the chamber this can impair the temperature distribution in the unit When loading the unit do not arrange articles too clo
36. nts V Press C s 0 CO Change circuit status PRA o Release setpoints A Press 4 C 5 CO Exit function level Actual temperature CO O and w0 Release rel humidity values are displayed again after 1 minute Press any setpoint key Actual temperature CO O and rel humidity values are displayed again immediately 30 43 50 079 041 6 OPERATE Switching function Door heater ON OFF To adapt the unit to the ambient temperature in the place of installation the door heater can be switched on or off see page 13 for example Ta Condensation can occur inside the unit especially on the glass door or glass screen when the door heater is switched off Factory setting door heater switched on Instruction Entry Keys Remarks Display Remarks Status Select function level 5 w Hold down Indicator lamp control flashes and the current function level is indi cated by the flashing display A or Y Press C Display circuit status Release and hold down e 6 factory setting 0 again C Door heater ON CO2 Change circuit status Cc o Door heater OFF V Press C nm co Change circuit status o Door heater ON A Press C EX co Exit function level O Release again after 1 minute Actual temperature CO O and rel humidity values are displayed Press any setpoint key
37. ook We recommend you keep an equipment log book refer to APPENDIX Keep a record of inspection and tests calibration work and any major work carried out on the unit e g maintenance work agents loaded etc in this log book Replacement of electrical parts Work on electrical components of the unit may only be carried out by Heraeus service de partment personnel and when the unit is in a deenergized state disconnected from the mains supply Only use original replacement parts approved by Thermo Electron Gmbh The sensors may only be replaced by authorized personnel of the operator 36 43 50 079 041 11 AUTHORIZED REPLACEMENT PARTS AND ACCESSORIES The safety and serviceability of the equipment are only guaranteed if the approved original replacement parts specified below are used Use of other parts presents unknown risks and is not approved under any circumstances Replacement part Type Order No Instruction manual 50 079 019 Hose set for connecting unit to gas supply 50 062 701 Unit foot upper part 50 044 921 Foot height adjustable lower part 50 029 587 2 support brackets 50 011 380 Shelf 50 069 923 Shelf divided 50 069 921 Glass door 50 025 445 Gasket for iner casing front 50 048 705 Glass door for gas tight glass screen left 50 030 020 Glass door for gas tight glass screen with feed 50 030 021 trough left Glass door for gas tight
38. ring operation Example Display 5 0 CO Comparative measurement 4 2 CO Instruction Entry Keys Remarks Display Remarks Status Select function level 3 Indicator lamp control flashes and 0 mole down the current function level is indi cated on the flashing display A or V Press mem c Display zero point Release and press C 0 again oft MO co Enter determined CO con o centration A or V Press C E co Start calibration process exit function level 10 Release i CAL is briefly displayed I Press The corrected actual value is then displayed 50 079 041 29 45 6 OPERATE Switching function Setpoint lock in ON OFF To prevent unintentional or unauthorized modification of the setpoints for temperature CO O and rel humidity the setpoints can be locked in The setpoints cannot be changed until the interlock has be cancelled again Factory setting setpoints unlocked Instruction Entry Keys Remarks Display Remarks Status Select function level 4 Hold down Indicator lamp control flashes and E 0 current function level is indicated by flashing display A or V Press C Display circuit status Release and hold down us o factory setting 0 again y C setpoints released j 5 CO2 Change circuit status o locked setpoi
39. rror code request The unit is equipped with an error diagnosis system This diagnosis system lets you detect and evaluate a fault during operation by means of specific codes If an error occurs within a control loop the relevant display flashes to indicate this Press page 19 18 to display the error detected by the diagnosis system and to acknowl edge the alarm When the error is cleared the error message is automatically closed and the display stops flashing Exception Error 502 Instruction Entry Keys Remarks Display Remarks Status Scan error code i Hold down Error code is displayed acoustic Acknowledge acoustic alarm alarm is acknowledged Display No error detected 24 43 50 079 041 6 OPERATE ERROR CODES CODE CAUSE Error condition POSSIBLE REMEDY 88 l Error during auto start Measuring cell outside cali Repeat the auto start routine refer to auto start bration range pages 23 Unit doors are open Doors are open for more Close the unit doors 99 Ls than 10 minutes refer to page 14 or just acknowledge gt 10 min acoustic alarm Temperature below Actual value lt setpoint Check the settings on the controller 100 setpoint 0 5 C and Temperature Limit Controller re fer to pages 18 and 20 Temperature above Actual value gt setpoint Ambient temperature could be too 101 setpoint 1 C high check the circ
40. se to one another on the shelves use only approx 70 of surface area in order to avoid impairing air circulation and en sure constant heat flow Do not impair temperature distribution This is mainly caused by the following m Covering the air vents Voluminous objects or apparatus Equipment releasing large amounts of heat Do not change the settings of the temperature protection devices as this will render load protection ineffective Operating interruptions If operations are interrupted make sure that no contamination hazards can occur Remove disinfect and clean any objects or auxiliaries put into the unit Disinfect and clean the chamber Disinfect and remove any residues Disinfect the unit with hot air disinfection routine 50 079 041 33 45 9 SHUTDOWN The unit may only be shut down by qualified staff trained to operate this equipment The unit must not present any risk of contamination after shutdown Shut off the gas supply Remove disinfect and clean any objects or auxiliaries put into the unit Disinfect and clean the chamber Leave the unit doors open until the chamber is dry m Disinfect the unit with hot air disinfection routine Set the mains switch to 0 and disconnect the power plug 34 43 50 079 041 10 MAINTENANCE Cleaning Disinfection Be sure to disinfect the unit in compliance with the applicable regulations in your country FRG ZH 1 598 The chamber and fittings
41. the unit door is opened e The unit door can only be closed when all glass doors are sealed properly If the unit door is ta not properly closed while the unit is in operation a visual alarm is given all LEDs flashing If the door is open for longer than 10 minutes an acoustic alarm sounds when this monitor ing function is activated by way of function level 1 refer to Page 26 Internal fittings Pull out shelves with support brackets to prevent tilting are arranged inside the units The sup port can be offset in the carrier racks at intervals of 46 mm Six piece gas tight glass screen By virtue of the small vent cross section unnecessary cooling and discharge of the atmosphere in the chamber is largely avoided when a glass door is opened of the six piece gas tight glass screen Lockable outside door The outside door is lockable Disinfection routine This routine is for disinfecting the interior of the unit In this process the unit is heated to 180 C and kept at the disinfection temperature for approx 3 hours The unit is then automatically cooled down again and the nominal values for temperature gases and relative humidity are reset The entire procedure lasts approx 13 hours after which the unit is ready for operation again Potential free contact connection Connection for the customer s own signalling system e g telephone system building services management systems If the overtemperature protection dev
42. tion O N GASSING optional O concentration optional HUMIDIFICATION Relative humidity Increase reading Decrease reading 11 des O autozero a O rH x 9 ro al auto start a control 11 Indicator lamp 12 Key 13 Indicator lamp 14 Key 15 Key 16 Key 17 Indicator lamp 18 Key 19 Indicator lamp 20 Key 50 079 041 12 13 14 15 16 17 18 19 20 Disinfection mode Temperature setpoint auto zero CO setpoint O setpoint optional Relative humidity setpoint auto start Error code inquiry Start calibration routines Control Switching functions 19 45 6 OPERATE In its as delivered condition the unit is preset to the following values Temperature 37 0 C CO concentration 0 0 O concentration 21 0 optional Relative humidity 60 0 humidity control activated via function level 2 Setting the temperature setpoint Instruction Entry Keys Remarks Display Remarks Status Switch on unit All display elements come on for Example EXE master switch I approx 15 seconds Auto test of controller Display temperature setpoint C press Actual setpoint is displayed and decimal point flashes Setpoint increases Select temperature setpoint 2 A press Setpoint decreases C C amp V press
43. tive humidity be Actual value lt setpoint 5 Check the water reservoir 400 KAI low setpoint Contact the service department of Thermo Electron Gmbh if necessary 404 Lo ro Relative humidity sen Sensor faulty short Contact the service department of AA sor faulty circuited Thermo Electron Gmbh 405 SK Evaporator faulty 95 C gt Actual value Contact the service department of 50 079 041 25 45 6 OPERATE des des temperature Actual value lt 180 C 10 C Repeat the disinfection process Con 500 below setpoint tact the service department of Thermo Electron Gmbh if necessary des temperature Actual value gt 180 C Contact the service department of 501 des above setpoint 10 Thermo Electron Gmbh Error during disinfec Power failure gt 1 sec Press the des buton Fig 4 7 to re 502 tion procedure set the error and repeat the disinfec des tion procedure check voltage supply for power failure A malfunction of the unit is not the only explanation for error codes 101 201 They can also be displayed after a setpoint is decreased Example When operating the unit with the setpoint entered for 10 CO If the CO setpoint is decreased to 5 CO the unit cannot reach this lower CO concentration quickly enough due to its tightness Error 201 is displayed The designated error messages are displayed when m Setpoint lt actual value m Setpointtemperatur lt ambient temperature
44. to the workplace before commencing work These instructions must be re issued at least once annually thereafter Employees inexperienced in the fields of microbiology virology or cellular biology must be given thorough instructions and be supervised Vermin and pests must be combatted regularly if necessary Handling of pathogenes Also heed the following basic rules regarding the handling of pathogenes Disinfect all workplaces on a daily basis It may be necessary to switch disinfectants as a precaution against resistant germs Do not wear protective garments outside the working areas Contaminated equipment must be autoclaved or disinfected prior to cleaning Waste containing pathogenes must be collected safely and rendered harmless through autoclaving or disinfection If infectious substances are spilt the contaminated area must be closed off immediately and disinfected If work is conducted using human pathogenes against which an effective antidote is available all employees who are not yet immune must be vaccinated and checked regularly for immunity in the appro priate manner The health of employees must be monitored by medical check ups i e employees must undergo an initial check up before commencing work and subsequent annual check ups Medical check ups should be conducted in accordance with the applicable employers liability insurance association guidelines in particular G 24 Skin diseases and G 42 Infectious d
45. uit status of the door heater refer to pages 15 and 31 Temperature sensor Sensor broken short Contact the service department of gt 620 C Thermo Electron Gmbh 104 faulty circuited Thermo Electron GmbH CO below setpoint Actual value lt setpoint 1 Check the gas supply for cylinder content 200 CO2 inlet pressure supply line and connection to unit Refer to page 12 CO above setpoint Actual value gt setpoint Check the gas supply for 201 Y 1 inlet pressure Refer to page 12 CO measuring cell Sensor broken short Contact the service department of AO 2 294 co faulty circuited Thermo Electron Gmbh Ns a N below setpoint Actual O value lt Check the gas supply for 2 setpoint 2 gas cylinder content 300 Gassing with N inlet pressure supply line and connection to unit Refer to page 13 14 N above setpoint Actual O value gt Check the gas supply for 301 OF pp setpoint 2 inlet pressure Begasung mit N Refer to page 13 14 Oe O above setpoint Actual O value lt Check the gas supply for setpoint 2 gas cylinder content 302 Gassing with O inlet pressure supply line and connection to unit Refer to page 13 14 Oo y O above setpoint Actual O value gt Check the gas supply for 303 setpoint 2 inlet pressure Gassing with O Refer to page 13 14 304 NL 24010 sensor faulty Contact service z Rela
46. urroundings at 37 C 95 kWh h approx 0 16 rel humidity Heat release into surroundings at 50 C 95 kWh h approx 0 22 rel humidity Heat release into surroundings during disinfec kWh h approx 0 59 tion process Recovery time at 37 C 5 CO 95 rel humidity 7 O closing the doors after they have been open for 30 s Temperature min lt 3 CO min lt 2 max 1 4 min Humidity min lt 9 O min lt 15 38 43 50 079 041 TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS UNITS VALUE Humidification Water quality Distilled or fully desalinated min max level l 2 2 Water demand to humidify from 40 rel humidity to 95 rel humidity ml 17 Water consumption ml h 10 Total capacity l 4 7 Measuring range and Setting range r H 160 95 Control accuracy rH 1 GAS ENGINEERING Carbon dioxide CO Oxygen O Nitrogen N Purity 99 5 Inlet pressure bar 0 8 up to max 1 Measuring range and Setting range CO 10 20 Control accuracy CO 0 1 ELECTRICAL Rated voltage V 1 PE AC 230 or 1 PE AC 120 Rated frequency Hz 50 60 50 60 Radio interference suppression DIN VDE Interference suppression level N 0875 Type of enclosure DIN 40050 IP 20 Protection class l Overvoltage category IEC 1010 II Degree of soiling IEC 1010 2 Rated current A 5 2 or 10 0 Fuse protection Fuse T 16 A slow blow
47. w cap off Hang the canister on the bracket lo cated on the upper left of the incubator fig 1 Connect the tubing connector fig 1 into the quick fit coupling the water reservoir will be filled under the force of gravity Should the water not flow there may be an airlock in the tube in which case press on the tube bulb to move the air through Attention If the reservoir is overfilled the excess water any will flow from the overflow pipe After filling place the filling canister on the floor and allow water in the filling tube to return to the canister before removing the quick fit connection fig 2 Attention After filling completely empty and dry the filling canister store the canister dry to prevent the formation of contamination Figure 1 Figure 2 50 079 041 6 OPERATE As the outlet not only functions as an overflow but also as a vent it must be unsealed while the unit is in operation PRD 7 La 7 To drain the empty filling canister place it on the floor and connect it using the quick release coupling 9 Take care that the filling canister does not overflow Disconnect the quick release coupling if necessary E Aways drain the water reservoir before transporting the unit Mains switch 0 Unit OFF Unit ON the green indicator lamp 3 indicates that unit is ready for operation Fig 5 Mains switch 8 50 079 041 17 45 6 OPERATE Temperature protection device temperature limit contro
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