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Model 680 Small Animal Ventilator Manual
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1. a pathway for the expired air Harvard Apparatus Model 680 Rodent Respirator Manual l Operation OPERATION Series 680 pumps are equipped with a three wire grounded cord The grounding should be kept intact Hook Up to Animal A y connection should be inserted between the connection to the animal cannula ec and the two tubes coming from the pump D ead space is minimized if the y is as close to the animal as possible See attached sketch Rate and Volume The stroke volume is adjusted by the crank knob at the top of the pump with the volume being indi cated on the cylinder graduations The rate is adjusted on the top panel motor control with knob Expired air can be collected at the exhaust top vertical tube Maintenance The entire pump mechanism is available for inspection by removing the bottom panel All bearings and points of sliding contact should be lubricated every 30 days with machine oil of 20 30 grade A finger full of the silicone lubricant should be smeared inside the cylinder and on cam surface every 90 days or so The motor should be lubricated in accordance with the motor manufacturer s direction Harvard Apparatus Model 680 Rodent Respirator Manual Operation Cont d Replacement Cylinders and Pistons Each replacement syringe cylinder and piston consists of a set cylinder and piston Catalog Number 683 684 685 682 Description Replacement 5cc cylinder and
2. Model 680 Small Animal Ventilator User s Manual 680 Small Animal Ventilator 55 3438 Harvard Apparatus Model 680 Rodent Respirator Manual Table of Contents SUBJECT PAGE NO Tableof CONE aNG aaa ALAN aar aeaii 1 General Information Warranty and Repairs u s 2 AA eT Te en ent rt eer re eet eet 3 Operation maa AABANGAN KUAN ad 4 6 CC FV EIN VUES essence tea wren AA GAAN 7 Volume MII eM svsiiississiniranrissiraniierraitianinnenaerantiasin ita taal tas aaaten asanedadeanaedentusbied 8 Harvard Apparatus Model 680 Rodent Respirator Manual l General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are locat ed on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency W hile the flow will stay calibrated the peak will vary Warranty H arvard Apparatus warranties this instrument for a period of one year from date of purchase At its option H arvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or mate rial This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSE QUENTIAL DAMAGES Some states do not allow exclusion or lim
3. an freely moving 10 cc glass syringe to a short 2 length of tubing and y connec tor Adjust the syringe plunger so that 1 or 2 cc of air isin the syringe 3 Attach the two arms of the y connector to the lower two ports of the valve block Use short lengths of tubing This will establish a closed system between the pump cylinder and the syringe so that the air will move back and forth from the pump to the syringe Minimum dead space between the pump ports and the syringe will assure an accurate test 4 Turn on the pump and observe the excursion of the syringe plunger as it moves back and forth Hold the syringe horizontally to minimize the effect of gravity on the plunger travel The limits of its travel will indicate the actual amount of air being supplied by the pump at each stroke 5 To check stroke volume use a free moving 50 cc glass syringe and connect with a single short rub ber tube to output one way valve port With pump piston in maximum stroke position run pump about 20 strokes min and observe how many strokes it takes to get to 50 cc i e On 5 cc pump stroke should take 10 strokes Should the foregoing tests show error in volume output of pump the most probable cause is air leakage or valve cycling adjustment T he air leakage can be in the o ring seals valve cylinder and piston If there is any doubt the o rings should be removed and new ones installed Use Repair Kit Cat No 682 To readjust slid
4. e valve is a more difficult problem The mechanism assembly must be removed from the chassis Follow instructions as in changing cylinders i e remove control panel and volume adjustment knob cylinder and piston Remove bottom cover Also remove four holding screws two on top of chassis and two on side DO NOT REMOVE VALVE ASSEMBLY Mechanism now should come out through the bottom of chassis By clamping right hand sidein a vise motor can be run and all further volume checks can be made Run motor and stop at the point where the high side of cam is pushing valve spindle and push rod to its uppermost position At this point on pressurizing 3 psi the room air inlet port there should be no leakage past the slide valve If bad leakage occurs the slide valve should be adjusted upwards To do this loosen check nut on pushrod and unscrew capscrew It should be moved just enough to shut off air intake When this condition is obtained all other port cycling should be automatically sequenced To check run motor to put cam lobe on lowest position On output port remove hex faced tube and remove spring and ball valve Replace hex faced tube and pressurize this port There should be no leakage past slide valve at this point If this slide valve assembly is ever removed from mechanism plate it should be rechecked for adjustment T he setting of the slide valve is quite critical to efficient pump operation All volumes should be rechecked as before
5. itation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WAR RANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limita tion may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RM A returned materials authorization num ber has been issued by our customer service department T he customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts H arvard Apparatus stocks replacement and repair parts W hen ordering please describe parts as complete ly as possible preferably using our part numbers If practical enclose a sample or drawing We offer a com plete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patien
6. olding control cover in place M ove control cover aside without disconnecting wires Loosen two 2 4 40 set screws on top of block on piston shaft Slide piston and shaft back towards bearings and then loosen two screws in valve block and remove cylinder an o rings Remove piston by holding hex fit ting next to piston and uncouple hex nut Do not lose neoprene spacers in nut Put back replacement pis ton in reverse manner as removing Couple replacement cylinder and o ring on valve block replace screws and push piston and rod to end Pull back about 1 32 for clearance Be sure crank is on furthest inward stroke Finally lock down block set screws on shaft flat replace cover and crank knob Plug cord 110 VAC in and your pump is ready to operate CAUTION Valve block should never be removed from mounting plate to change cylinders and pistons To do so may derange proper valve air cycling Harvard Apparatus Model 680 Rodent Respirator Manual Volume Calibration PROCEDURE If you have reason to suspect that the output volume of the pump at any given setting is not comparable to the graduation marks on the pump cylinder here is a simple accurate method for testing calibration 1 Set the speed control dial to about 20 strokes per minute Set the pump piston for a volume of 5 cc and stop the pump motor when the piston reaches its most rearward position that is when the cylinder contains 5 cc of air 2 Attach a cle
7. piston for Model 680 only Replacement 10cc cylinder and piston for Models 680 and 681 Replacement 10cc cylinder and piston for Model 681 only Repair Kit for 680 681 Diagram 1 M ode 680 Rodent Respirator Valve H ook U p to Animal Y Connection as close to Tube animal as possible Output one way valve Connection SP Animal Expired Air i Y Pump Air a C f Room Air or j cT Non explosive Mixture Cylinder aD Say Valve Block L e Recycling 680 Rodent Respirator Manual Harvard Apparatus Model Section Drawing Diagram 2 Section Showing Cam at H ith Point NG 0 Valve Block 0680 145 BAG 5013 017 0680 067 5013 017 BOC 5 37 019 5009 037 me 6 a ol NO Ime SSS M Piston NASI 5020 182 a 5009 035 Cylinder 5114 001 gt 5013 027 30cc ows 5013 018 10cc i 5013 019 5cc 5011 015 Oo 5008 014 H SZ Harvard Apparatus Model 680 Rodent Respirator Manual l Changing Cylinders INSTRUCTION CAUTION UNPLUG UNIT To change different size cylinders remove crank knob in top of unit the 6 screws h
8. ts CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS Harvard Apparatus Model 680 Rodent Respirator Manual Description Series 680 Constant Volume Ventilators are positive pressure pumps designed for small rodents T hey con sist of a shunt wound motor operated by a solid state motor speed control linkages cylinder and piston assembly and cam operated slide valve All of the mechanics and electronics are contained in a sheet metal housing and mechanism plate The linkages are so designed the piston almost touches the end of the cylinder at each stroke regardless of the stroke volume T his insures that virtually all of the air taken into the pup is expelled at each stroke The volume is adjustable while the pump is running by means of a crank knob on the top Volume is indicated by graduation marks on the transparent cylinder The pump is designed to use one of two different sized pistons and cylinder In both cases the range of pis ton travel 5 c m is the same Model 680 is furnished with one 5 cc and one 10 cc cylinder and piston assembly M odel 681 is furnished with one 10 cc and one 30 cc cylinder and piston assembly T he larger cylinder is factory installed and the smaller supplied separately A three way slide valve is attached to the mechanism plate at end of cylinder and actuated by a cam on the motor The bottom port is the room air input the middle port is the pump air output while the two upper ports are
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