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AKTAdesign Monitor pH:C900 Manual

1. Check Service Mode Telephone Service 012345678901 Contract Number 012345678901 Serial Number 01234567 YM 012345 Monitor pH C 900 v1 00 Date of Maintenance Buzzer Test 30 B 1 Check menu B 1 1 Check Monitor Cell and Electrode A check of the conductivity cell and pH electrode can be performed 1 Select main menu Check press OK 2 Select sub menu CheckMonitor cell and Electrode press OK 3 Thetext Passed will appear for 2 seconds if the cell and electrode are indicating reasonable values If there are any problems an error message will appear B 1 2 Check Service Mode Service information relevant to the instrument can be checked Information may not be available in all menus 1 Select main menu Check press OK 2 Select sub menu Check Service Mode press OK 3 The service telephone number is displayed press OK 4 The service contract number is displayed press OK 5 The instrument serial number is displayed press OK 6 The instrument name and software version are displayed press OK 7 The date of the last service is displayed press OK 8 Atest of the instrument buzzer is performed press OK Monitor pH C 900 User Manual 18 1120 06 Edition AF Setup Conductivity Setup Cond Temp Comp 2 0 C 2 0 Setup Cond Ref Temp 22 3 C 25 0 Reference information B B 2 Setup menu B 2 1 Setting up conductivity temperature
2. pega villas 6 Awarning message will be displayed press OK eo 280 7 Enter the temperature shown on the thermometer press OK B 2 7 Setting up the temperature display Display of the temperature in the conductivity flow cell in the main operating menu can be enabled or disabled 1 Select main menu Setup press OK Setup Temperature 2 Select sub menu Setup Temperature press OK Setup Show Temp 3 Select sub menu Setup Show Temp press OK On On Off 4 Set the temperature display on or off press OK 34 Monitor pH C 900 User Manual 18 1120 06 Edition AF Setup Language GB GB DF EI Setup Unit Number Set Display Angle Reference information B B 2 8 Selecting language The language used on the display can be changed 1 Select main menu Setup press OK 2 Select sub menu Setup Language press OK 3 Select the desired language GB British English D German F French E Spanish Italian B 2 9 Setup unit number The unit number is the identification the Monitor pH C 900 has on the UniNet bus It should correspond to the number set in UNICORN for the Monitor pH C 900 The number should be set to 0 if one pH C 900 is used If more than one pH C 900 monitor is used they must all have different numbers 1 Select main menu Setup press OK 2 Select sub menu Setup Unit Number press OK 3 Select unit number 0 25 B 2 10 Setup display angle The d
3. o d 1 16 2m 18 1112 54 PEEK tubing i d 1 0 mm o d 1 16 2m 18 1115 83 Fingertight connector 1 16 10 18 1112 55 Monitor pH C 900 User Manual 18 1120 06 Edition AF 41 Short instructions Short instructions Calibrating Cond Temp pH 25 4 22 9C 11 5 Calibrate pH Set Cond Scale 100 42 The following short instructions are checklists for users who are fully familiar with the safety precautions and operating instructions described in this manual The instructions assume that optional equipment is installed according to the installation instructions Select 1 Switch on the instrument by means of the mains switch on the rear panel 2 The main operating menu RUN menu is shown 3 Calibrate the pH electrode before use and or daily by using 2 buffers with known pH values 4 The conductivity flow cell does not normally need to be calibrated 5 Set the conductivity scaling for 0 and 100 6 Aways store the pH electrode in 1 1 mixture of pH 4 buffer and 1 M KNO3 when not in use Monitor pH C 900 User Manual 18 1120 06 Edition AF For contact information for your local office please visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company KTA Drop Design AKTApurifier AK
4. 2 3 2 3 2 2 Return to main menu Pressing ESC repeatedly always returns to the main menu 1 which is the main operating menu Main menu 1 ESC ESC ESC ESC Main menu 2 Select value A cursor below a text or numerical value shows what is affected by the dial To increase the value turn the dial clockwise To decrease the value turn the dial counterclockwise The value can be reset by turning the dial several clicks counterclockwise Parameter Setup Cond ref temp Current value Newvalue to be set When setting numerical values the cursor moves up to the next digit if the dial is turned quickly in one direction 22 0 to simplify entering large values The cursor moves back one place to the right every two seconds if the dial is not turned The text or numerical value displayed is accepted by pressing the OK button To cancel press the ESC button Monitor pH C 900 User Manual 18 1120 06 Edition AF Cond Temp pH 25 4 22 9 C 11 50 4 000 mS cm 22 9 C Set Cond Scale 100 Set Cond Scale 0 Calibrate pH Set pH Analogue Out Check Setup Alarm Timer 00 30 52 Cond Temp pH 25 4 22 9 C 11 50 or CondTc Temp pH 25 4 22 9 C 11 50 Tc 4 000mS cm 22 9 C Bar graph Operation 3 3 3 Main menu overview Main operation menu The menu is accessed from all posi
5. Handle the pH electrode with care CAUTION The tip of the pH electrode consists of a thin glass membrane Protect it from breakage contamination and drying out or the electrode will be destroyed Always store the electrode with the end cover filled with a 1 1 mixture of pH 4 buffer and 1 M KNO3 Do NOT store in water only 1 Unpack the pH electrode Ensure that it is not broken or dry 2 Prior to first using the electrode remove the electrode end cover and immerse the glass bulb in buffer for 30 minutes 3 Remove the dummy electrode from the flow cell and store it in the flow cell holder 4 Carefully insert the electrode in the flow cell Tighten the nut by hand to secure the electrode Note If the electrode is not fully inserted the system will leak and a dead volume will occur in the holder 5 Connect the pH electrode cable to the rear of the instrument to the socket pH Probe 2 6 Connecting electrical signal cables WARNING Only use mains cables delivered or approved by GE Healthcare The sockets for electrical signals are located on the rear panel pH pH Ground Probe Analogue out 0 1V Mains On off Conductivity H Cond Flow Cell DS HET 0 O0 m 2 7 Connecting to chart recorder if used The external chart recorder outputs for pH and conductivity from the monitor are 0 1 V There is also a 4 20 mA signal available at the same output connectors Monitor pH C 900 User Manual 18 1
6. The new cell constant is automatically calculated and updated Conductivity of 1 00 M NaCl at 20 30 C 97 95 90 Conductivity mS cm 85 80 77 Taranaratiirn Ir Monitor pH C 900 User Manual 18 1120 06 Edition AF Setup Conductivity Setup Adj Cell Const 34 4 cm Warning This will change cell calib Setup Adj Cell Const 34 4 cm 35 5 Setup pH Setup pH Temp Comp Off On Off Reference information B 8 2 3 Entering the conductivity cell constant After replacing the flow cell the cell constant has to be set The cell constant is shown on the packaging 1 Select main menu Setup press OK 2 Select sub menu Setup Conductivity press OK 3 Select sub menu Setup Adjust Cell Constant press OK 4 A warning message will be displayed press OK 5 Enter the cell constant press OK The new cell constant is updated When entering the cell constant from UNICORN select System Control System Calibrate and select Cond_Cell B 2 4 Setting up pH temperature compensation The relationship between pH and the output signal from the pH electrode is temperature dependent For more accurate measurements during temperature changes the pH measurement can be temperature compensated In normal applications if the temperature of the buffers and calibration buffers are identical temperature compensation does not need to be on When using temperature compensation
7. compensation The conductivity in a buffer is temperature dependent To relate conductivity to concentration and or compare conductivity values temperature compensation should be used The compensation consists of a compensation factor together with a reference temperature All conductivity values will then automatically be converted to the set reference temperature 1 Select main menu Setup press OK 2 Select sub menu Setup Conductivity press OK 3 Select sub menu Setup Cond Temp Comp press OK 4 Set a temperature compensation factor press OK The factor is expressed in percentage increase of conductivity per C increase in temperature If the temperature compensation factor is unknown a general approximate value of 2 can be set for many common salt buffers Set the value to 0 for no temperature compensation 5 Select sub menu Setup Cond Ref Temp press OK Select the reference temperature to which the measured conductivity values will be converted normally 20 or 25 C press OK B 2 2 Calibrating the conductivity cell Normally it is not necessary to adjust the cell constant as the flow cell is pre calibrated on delivery Adjustment is only necessary when replacing the conductivity flow cell with a flow cell whose cell constant is unknown It is also recommended that the conductivity flow cell is recalibrated after cleaning When adjusting the cell constant from UNICORN select System Control System Calibrate and then se
8. installed on a stable laboratory bench providing a suitable working area gt gt be Monitor pH C 900 User Manual 18 1120 06 Edition AF 7 2 Installation 2 Installation A 2 1 Unpacking CAUTION The following information should be read carefully to ensure that the instrument is installed correctly Unpack the instrument and check the items against the supplied packing list Inspect the items for obvious damage which may have occurred during transpor tation It is recommended that all packing materials should be retained if onward trans port of the instrument is expected 2 2 General precautions WARNING Do not block the rear panel of the system The mains power switch must always be easy to access WARNING Do not block the rear panel of the system The mains power switch must always be easy to access The instrument should be located in a place of low temperature variations away from heat sources draughts and direct sunlight The instrument shall be operated within its normal ambient temperature range 4 to 40 C The instrument should be installed on a stable laboratory bench or in AKTAexplorer or AKTApurifier To ensure correct ventilation a free space of 0 1 mis required behind and in front of the instrument Do not use any soft mate rial under the instrument to ensure that the ventilation inlet in the front is not blocked CAUTION The mains power to pH
9. it is important that the temperature of the pH electrode is the same as that of the conductivity flow cell since that is where the temperature is measured 1 Select main menu Setup press OK 2 Select menu Setup pH press OK 3 Select sub menu Setup pH Temp Comp press OK 4 Set the temperature compensation on or off press OK Monitor pH C 900 User Manual 18 1120 06 Edition AF 33 B Reference information B 2 5 Setting up the pH display Normally the pH is displayed in the main operating menu or its alternative see section 3 4 Reading pH and conductivity values If not required the pH display can be set to off 1 Select main menu Setup press OK one Show pH 2 Select menu Setup pH press OK 3 Select sub menu Setup Show pH press OK 4 Set the pH display on or off press OK B 2 6 Calibrating the temperature sensor Calibration of the temperature sensor in the conductivity flow cell is only necessary if the monitor is used in high accuracy measurement or the conductivity flow cell is replaced 1 Place the flow cell together with a precision thermometer inside a box or empty beaker to ensure that they are not exposed to draft Leave them for 15 minutes to let the temperature stabize 2 Read the temperature on the thermometer 3 Select main menu Setup press OK Setup Temperature 4 Select sub menu Setup Temperature press OK eee eae 5 Select sub menu Setup Adjust Temp press OK
10. 120 06 Edition AF Installation 2 1 Connect the chart recorder to the DIN socket pH or Cond using the cable supplied a For 0 1 V use wire 1 and 2 Use a recorder with floating inputs i e none of the inputs connected to ground b For 4 20 mA use wire 5 and wire 6 Note Thesignal cable is delivered with protective covers on each wire Do not remove the protective covers from unused connections as a short circuit may disturb the measurements 2 Set the recorder to 0 1 V or 4 20 mA input full scale 2 8 Connecting to communication link The monitor is intended for use with AKTAexplorer and AKTApurifier and can be controlled from a PC running UNICORN version 2 20 or higher using UniNet cables Connect two UniNet cables to the UniNet 1 connectors The instrument can be connected in series anywhere between the PC and a termination plug The UniNet 1 link connects in series the PC with Pump P 900 Monitor pH C 900 Monitor UV 900 and the Frac 900 The termination plug is connected to the last instrument in the chain CAUTION The mains power to the monitor must be switched OFF before connecting the instrument to the UniNet 1 link 2 9 Connecting to supply voltage 1 Make sure the on off switch is in the OFF position O 2 Connect the supplied mains cable between the instrument and a grounded mains socket Any voltage 100 240 V AC 50 60 Hz can be used WARNING The instrument must be connected
11. C 900 must be switched OFF before connecting the instrument to any cells or external equipment 2 3 Installing the conductivity cell 1 Place the conductivity cell in a suitable place for example on the shelf of the Monitor UV 900 using the clip provided with the UV monitor The cell can be placed up to 1 5 m from the monitor housing Monitor pH C 900 User Manual 18 1120 06 Edition AF Installation 2 2 Connect the conductivity cell to the socket Conductivity Flow Cell on the rear panel of the instrument si 3 Connect the tubing with the Fingertight connectors The flow cell itself does not have a recommended flow direction In conjunction with the pH electrode place the conductivity flow cell and select its flow direction so that the screw head end of the flow cell faces the pH flow cell 2 4 Installing the pH electrode 2 4 1 Mounting the flow cell holder 1 Hook the flow cell holder on the right hand side of the housing Secure it with the slide clamp A amp Flow direction If the flow cell holder is not used the flow cell must still be installed at an angle of 30 from the vertical with the outlet placed higher than the inlet to prevent air bubbles being trapped in the cell The flow direction is marked on the flow cell 2 Connect the tubing with the Fingertight connectors Monitor pH C 900 User Manual 18 1120 06 Edition AF 9 2 Installation 10 2 5 Inserting the pH electrode Note
12. GE Healthcare AKTAdesign Monitor pH C 900 User Manual 36 KTA Important user information CE Certification All users must read this entire manual to fully understand the safe use of AKTAdesign Monitor pH C 900 IMPORTANT KTAdesign Monitor pH C 900 is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes WARNING The Warning sign highlights an instruction that must be strictly followed in order to avoid personal injury Be sure not to proceed until the instructions are clearly understood and all stated conditions are met CAUTION The Caution sign is used to call attention to instructions or conditions that must be followed to avoid damage to the product or other equipment Be sure not to proceed until the instructions are clearly understood and all stated conditions are met Note The Note sign is used to indicate information important for trouble free and optimal use of the product Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please EE Contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a class A product In a domestic environment it may cause radio interference in which case the user may be required to take appropriate measures
13. H Setup Temperature Setup Language Setup Unit Number Set Display Angle Alarm Timer 12 30 52 Set Alarm Set Timer Set Clock Alarm Timer off Monitor pH C 900 User Manual 18 1120 06 Edition AF 01224567ean1 Serial Number O1224647 VMNIQZAE Monitor pH C 900 VT Date of Maintenance z Buzzer Test Setup Cond Temp Comp 2 0 Setup Cond Ref Temp 22 3 C Setup Adjust Cond 34565mS cm Setup Adj Cell Const 34 4cm 1 Setup pH Temp Comp Off Setup Show pH On Setup Adjust Temp 23 5 C Setup Show Temp On 37 C Reference information C Technical specifications The full specifications apply only after at least 1 hour warm up Operating data pH measurement pH range Accuracy temperature compensated not temperature compensated Response time Long term drift Flow rate sensitivity 0 to 14 spec valid between 2 and 12 0 1 pH within 4 to 40 C 0 2 pH within 15 to 25 C 0 5 pH within 4 to 15 C and 25 to 40 C Max 10 s 0 95 of step Max 0 02 pH h measured at pH 4 0 Max 0 1 pH units within 0 10 ml min Conductivity measurement Conductivity range Deviation from theoretical conductivity Reproducibility short term long term Noise Response time Temperature sensor Accuracy Drift Flow rate sensiti
14. H 4 buffer and 1 M KNO3z when not in use When the pH electrode is removed from the flow cell a dummy electrode can be inserted in the flow path Electrode regeneration If the electrode has dried out immerse the lower end of the electrode in buffer with a 1 1 mixture of pH 4 buffer and 1 M KNO overnight 3 10 Restart after power failure If the power supply to the instrument is interrupted the instrument automatically restarts itself and displays the main operating window All set values are retained in the instrument Monitor pH C 900 User Manual 18 1120 06 Edition AF 19 4 Maintenance 4 Maintenance A A A 20 WARNING Disconnect the power supply before attempting to replace any item on the instrument during maintenance CAUTION Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the instrument 4 1 Periodic maintenance WARNING Remove liquid or dirt from the system surface using a cloth and if necessary a mild cleaning agent Do not use strong detergents WARNING When using hazardous chemicals make sure that the entire system has been flushed thoroughly with bacteriostatic solution e g NaOH and distilled water before service and maintenance Interval Action see procedures below Every 6 month or more often if required Change pH electrode Check that the power cord is whole and undamaged It shall be replaced if any sig
15. Set pH Analogue Out Set pH Full Scale pH12 00 11 00 Set pH Zero Level pH 2 00 4 00 18 As a rule when an electrode has an asymmetry potential outside of 60 mV and a slope lower than 80 and no improvement can be achieved by cleaning it should be replaced An electrode is still usable at lower slopes and higher asymmetry potentials but the response will be slower and the accuracy diminish 3 6 2 Calibrating with the electrode in the flow cell When calibrating with the electrode fitted in the flow cell in AKTApurifier follow the above procedure Before adjusting the pH monitor ensure that the pH has stabilized Leave the pump running while calibrating Switch to the other standard buffer solution and repeat the procedure For a description of calibration from UNICORN with the electrode fitted in the flow cell see section 6 6 in UNICORN User Manual 3 7 Calibrating conductivity The cell constant for the particular flow cell is written on the flow cell packaging Refer to section B 2 3 in Reference information for how to enter the cell constant Adjustment of the cell constant is only necessary when the monitor is to be used to determine specific conductivity with high accuracy The procedure is described in Reference information section B 2 2 Calibration can also be performed from UNICORN 3 8 Using an external chart recorder The external chart recorder outputs for pH and conductivity from
16. TAexplorer and UNICORN are trademarks of GE Healthcare companies All third party trademarks are the property of their respective owners 1996 2008 General Electric Company All rights reserved First published Sept 1996 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 18 1120 06AF 06 2008
17. WARNING All repairs should be done by personnel authorized by GE Healthcare Do not open any covers or replace any parts unless specifically stated in the instructions Monitor pH C 900 User Manual 18 1120 06 Edition AF This product complies with the European directives listed below by fulfilling corresponding standards A copy of the Declaration of Conformity is available on request e 73 23 EEC Low Voltage Directive e 89 336 EEC EMC Directive The CE logo and corresponding declaration of conformity is valid for the instrument when it is e used as a stand alone unit or e connected to other CE marked GE Healthcare instruments or e connected to other products recommended or described in this manual and e used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Note The Declaration of conformity is valid only for systems that are marked with the CE logo CE Contents Contents CONLENES oon cccs adesessess daslssoss less ss cannsusenndeseawinsesusenesetcedaveesders 4 IntroductioN ioiii 6 ZAREE Ee EAEE EAEE E E A 6 2 2 SAFETY sates r a EA R A n n 6 2 30 WMPOGKING Seatac ta aA E A E A O EER 8 24 General PreCqutiOns nri A A i at 2 5 Installing the conductivity cell 2 6 IAst lling th pH electrode isanne ZT Insertingithe PH electrode einernie E E nette 2 8 Connecting electrical signal cables oo ssssssssssessecscccecceceessssessnsss
18. be at least 1 mS cm The settings remain until they are changed Values above 150 are shown as 150 values below 0 as 0 3 6 Calibrating pH A good laboratory routine is to calibrate the instrument once a day when the electrode is replaced and if the ambient temperature is changed The pH monitor is calibrated using standard buffer solutions in a two point calibration The two buffer solutions can have any pH value as long as the difference between them is at least 1 pH unit Calibration can also be performed from UNICORN In UNICORN select System Control System Calibrate Select the pH monitor The calibration procedure can be done with the pH electrode either fitted in or removed from the flow cell 3 6 1 Calibrating with the electrode outside the flow cell When calibrating the electrode out of the flow cell and changing from one buffer to another rinse the electrode tip with distilled water and dab it carefully with a soft tissue to absorb the remaining water Do NOT wipe the electrode as this may charge it and give unstable readings The steps below describe the procedure used with the electrode removed from the flow cell Monitor pH C 900 User Manual 18 1120 06 Edition AF Calibrate pH Calib pH Buffer 1 Calib pH Buffer 1 Calibrated Electrode Operation 3 Note The Monitor must be unlocked if connected to a UNICORN control system 1 Remove the pH electrode from the flow cell and imme
19. d clean the electrode following the instructions in section 4 3 Cleaning the pH electrode If the membrane has dried out the electrode may be restored by soaking it in buffer overnight Clogged liquid junction Refer to section 4 3 Cleaning the pH electrode Incorrect unstable pH reading Monitor pH C 900 User Manual 18 1120 06 Edition AF Check that the electrode cable is connected properly to the rear of the instrument Check that the pump and valves operates correctly Check that the electrode is correctly inserted in the flow cell and if necessary hand tighten the nut If air in the flow cell is suspected tap the flow cell carefully or tilt it to remove the air Alternatively flush the flow cell with buffer at 8 ml min for 1 2 min Check that the pH electrode is not broken Check that the pH electrode is calibrated 23 5 Trouble shooting 24 Fault Action 7 Check the slope see section 3 6 Calibrating pH If it is outside the range 80 105 or the asymmetry potential deviates more than 60 mV from O mV clean the pH electrode Recalibrate and if the problem persists replace the pH electrode Clean the pH electrode if required see section 4 3 Cleaning the pH electrode Compare the response of the pH electrode with that of another pH electrode If the response differ greatly the electrode may require cleaning or replacement 10 There may be interference from other equipment Connect the pH flo
20. display in section B 2 7 of Reference information 3 5 Setting conductivity scale The monitor measures conductivity over the complete working range No range settings are required However to obtain a usable output signal for a recorder or to view the conductivity as a percentage of buffer B it is possible to set a 0 to 100 Monitor pH C 900 User Manual 18 1120 06 Edition AF 15 3 Operation Set Cond Scale 100 Set Cond Scale 0 16 range This can be done with reference to the buffers used or by selecting any fixed range between 0 US cm and 999 9 mS cm To set the scale with reference to the buffers do as follows 1 Start the flow with the high conductivity buffer Select main menu Set Cond Scale 100 press OK 2 When the conductivity level has stabilized set the value which should correspond to 100 by pressing OK If required the value can be changed with the setting dial 3 Change to the low conductivity buffer Select main menu Set Cond Scale 0 press OK 4 When the conductivity level has stabilized set the value which should correspond to 0 by pressing OK If required the value can be changed with the setting dial The scale can be set without pumping buffer through the cell Follow the same procedure and simply set the conductivity values directly at points 2 and 4 ignoring the measured conductivity values displayed The difference between the value for 100 and 0 value span must
21. e e High accuracy and reproducibility e Flow cells with low dead volume e Accurate and reliable monitoring through self test and self calibration e Flow cells can be connected close together minimizing band broadening and time delay between detectors 1 2 Safety IMPORTANT Monitor pH C 900 is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes e The instrument is designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water e Operate in accordance with local safety instructions WARNING The instrument must not be opened by the user It contains high voltage circuits which can be capable of delivering a lethal electric shock WARNING Always disconnect the power supply before doing any maintenance Monitor pH C 900 User Manual 18 1120 06 Edition AF Introduction 1 WARNING The instrument must be connected to a grounded mains socket WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system WARNING Make sure that the pressure from the pump never exceeds the maximum cell pressure to avoid the risk of explosion See specifications for maximum pressure tolerance WARNING The system should be
22. e is still displayed replace the pH electrode Check temp amp cond condition Check that the conductivity cell is connected Diff cond 0 amp 100 is too small The difference between 0 and 100 must be at least 0 1 mS cm Recalibrate Only allowed in stand alone ERROR 88 89 96 Unit number cannot be changed when the instrument is connected in AKTAexplorer or AKTApurifier Call for Service ERROR key OK ERROR key Esc ERROR key OK Esc 1 Switch off the instrument 2 Check all connections 3 Switch on the instrument ERROR 100 ERROR 109 113 ERROR 120 Call for Service ERROR 121 Calibration range not within limits Recalibrate with new values ERROR 106 108 ERROR 118 Exc x y in ab c Exc DIV O in ab c Exc instr in ab c Exc address in ab c Switch off the instrument Check all UniNet1 connections Switch on the instrument Switch off the instrument Check all connections Switch on the instrument WM HI RM FR Monitor pH C 900 User Manual 18 1120 06 Edition AF Reference information A A Description A 1 Instrument Monitor pH C 900 is an on line monitor for measurement of pH and conductivity The monitor can work with standard glass pH electrodes with a built in liquid filled reference electrode and BNC connector pH pH Ground Probe Conductivity H Cond Flow Cell 6 Unei ow 00 wiz Analogue out 0 1V Mains On off The conduct
23. ear of the instrument housing 1 If the cell is replaced with a new flow cell the monitor must be calibrated with the new cell constant value which is written on the flow cell package See sub menu Setup conductivity in section B 2 3 in Reference information If the cell constant is not known it can be determined see section B 2 2 in Reference information 4 7 Instrument housing Wipe the instrument housing regularly with a damp cloth Let the instrument dry completely before use Monitor pH C 900 User Manual 18 1120 06 Edition AF Trouble shooting 5 5 Trouble shooting Monitor pH C900 A 5 1 General When contacting GE Healthcare for support state the program version of the instrument which is shown for 2 seconds during switch on voltage circuitry WARNING Do not open or remove enclosure Risk of electrocution from high 5 2 Faults and actions If the suggested actions do not correct the fault call GE Healthcare 5 2 1 pH measurement Fault Action No response to pH changes 1 Check that the electrode cable is connected properly to the rear of the instrument The electrode glass membrane may be cracked Replace the electrode Small response to pH changes Clean the pH electrode according to section 4 3 and recalibrate If the problem remains replace the pH electrode Slow pH response or Calibration impossible Check the pH electrode tip If it is contaminate
24. es resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system 3 1 On off Switch on the instrument at the mains switch on the rear panel At switch on the instrument performs a self test and then starts calibration After approx 30 seconds the display shows Cond Temp pH and the instrument is ready to use All parameters are factory set to default values If the conductivity cell is not connected the Temp and Cond fields are blank The pH field is blank if Show pH is set to off The monitor can be used immediately but the full specifications are not obtained until after a 1 hour warm up 3 2 Menu selection and settings 3 2 1 Moving between menus A specific menu is selected by turning the front selection dial clockwise or counterclockwise When the required menu is visible the menu or selection is accepted by pressing the OK button Menu selection OK button ESC button Select sub menu Return one menu level Monitor pH C 900 User Manual 18 1120 06 Edition AF 13 3 Operation 14 If a menu has sub levels the sub menu is displayed by pressing the OK button Pressing the ESC button moves back one menu level Main menus Sub menus Sub menus Main menu 1 Main menu 2 OK gt Sub menu 2 1 Sub menu 2 1 1 ESC Main menu 3 Sub menu 2 2 Main menu 4 CHENE K gt Sub menu 2 3 1 Main menu 5 Sub menu
25. etrahydrofuran THF Physical data Control Degree of protection housing flow cells Power requirements Power consumption Functions pH electrode cable length Cond cell cable length Inlet and outlet tubing Analogue outputs Display Dimensions H x W x D Weight Compliance with standards Saftey standards Stand alone or from a PC with UNICORN version 2 20 or higher through UniNet 1 connection P 20 P 44 100 240 V AC 50 60 Hz 25 VA Languages selectable English German Spanish French Italian 1 5 m BNC connector 1 5 m D sub 9 pole connector UNF 10 32 2B Fingertights with capillary tubing 1 16 outer diameter 0 1 V and 4 20 mA full scale overrange function see section 2 Installation for pin configuration 2 rows with 20 characters each 100 x 260 x 370 mm 8 5 kg The declaration of conformity is valid for the instrument only if it is e used in laboratory locations e used in the same state as it was delivered from GE Healthcare except or alterations described in the User Manual e connected to other CE labelled GE Healthcare modules or other products as recommended This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC and other international requirements through the following harmonized standards e EN 61010 1 e IEC 61010 1 e CAN CSA C22 2 No 61010 1 e UL61010 1 Monitor pH C 900 User Manual 18 1120 06 Edition AF 39 C Reference inf
26. isplay angle can be set to compensate for different viewing heights 1 Select main menu Setup press OK 2 Select sub menu Set Display Angle press OK 3 elect viewing angle gt Up gt Mid or gt Down Monitor pH C 900 User Manual 18 1120 06 Edition AF 35 B Reference information Alarm Timer 12 30 52 Set Alarm 12 32 21 0 00 00 00 Set Timer 18 34 52 00 00 00 Alarm Timer 12 35 16 Bzz00 33 00 Bzz00 00 29 13 08 45 Alarm time Set Clock 12 26 53 12 36 53 Alarm Timer off OK off Insert Access Code 36 B 3 Setting and using the alarm timer You can set the alarm function to either a fixed alarm time or using a count down timer The default or previous value is shown in parentheses 1 Select main menu Alarm Timer press OK The display shows the current time 2 Select sub menu Set Alarm if you want to set an alarm at a fixed time Press OK to enter the time value in the form HH MM SS pressing the OK button after entering each time unit 3 If you want to set a count down timer turn the dial to select sub menu Set Timer Press OK to enter the countdown value in the form HH MM SS pressing the OK button after entering each time unit An alarm time and a count down timer cannot both be set 4 Press ESC button to return to the Alarm Timer menu which now shows the set alarm time or count down time as BzZHH MM SS 5 When the alar
27. ivity monitor has a very large dynamic range from 1 uS to 999 9 mS cm and is therefore suitable for a wide range of applications Connector switch Function pH Ground Signal ground pH Probe Connection to pH electrode standard BNC socket Conductivity Flow Cell Connection to conductivity flow cell 9 pole D sub connector Analog out 0 1V 2 separate recorder outputs for pH and conductivity Recorder output 0 1 V UniNet 1 Computer network Mains Supply voltage grounded On off Instrument on off switch The instrument contains no internal user replaceable items Monitor pH C 900 User Manual 18 1120 06 Edition AF 27 A Description 28 A 2 pH electrode The pH electrode is of the sealed combination double junction type It contains a sealed Ag AgCl reference which cannot be refilled an internal electrolyte bridge of 4M KCl saturated with Ag AgCl an outer electrolyte bridge of 1M KNO3z an annular ceramic reference junction and a low profile pH membrane The pH electrode is delivered with a transparent cover Sealing Internal electrolyte bridge containing 4M KCI saturated with AgCl Silicone sealing Ag AgCl reference electrode Internal annular coaxial ceramic reference junction External electrolyte bridge containing 1M KNO3 NSSS SSSR RE EXTFFTXSZO SSSR aus Ss xv S O ring Glass electrode containing diluted buffered KCI O ring External annular c
28. lect CondCalib Note The conductivity temperature compensation must not be used when adjusting the cell constant Set the Setup Cond temp comp to 0 see section B 2 1 The temperature sensor must be calibrated before adjusting the cell constant see section B 2 6 1 Prepare a calibration solution of 1 00 M NaCl 58 44 g l Let the solution stand until it is at room temperature This is important for exact measurements 2 Fill the flow cell completely with the calibration solution by pumping at least 15 ml through the cell with a syringe 3 Stop the flow and wait 15 minutes until the temperature is constant in the Monitor pH C 900 User Manual 18 1120 06 Edition AF 31 B Reference information Setup Conductivity Setup Adjust Cond 83 53mS cm Warning This will change cell calib Setup Adjust Cond 83 53mS cm 86 60 32 range 20 30 C 4 Read the conductivity value displayed and compare it with the theoretical value from the graph on opposite page at the temperature of the calibration solution If the displayed value and the theoretical value correspond no further action is required If the values differ proceed with actions 5 8 5 Select main menu Setup press OK 6 Select sub menu Setup Conductivity press OK 7 Select sub menu Setup Adjust Cond press OK 8 Awarning message will be displayed press OK 9 Enter the theoretical conductivity value according to the graph press OK
29. m time is due or the count down timer reaches 00 00 00 an alert display is shown and the instrument beeps until the OK button is pressed The display shows the time elapsed since the alarm and the current time The alarm timer is based on the internal instrument clock which can be set in the Set Clock menu placed after the Alarm Timer menu The clock is reset when the power is turned off A set alarm timer function can be reset by pressing OK in the menu Alarm Timer off B 4 Service displays The instrument has service displays for use by authorized service personnel If the service display Insert Access Code is accidentally selected press the ESC button to exit to the normal operation display Monitor pH C 900 User Manual 18 1120 06 Edition AF Cond Temp pH 25 4 22 9 C 11 5 4 000 mS cm 22 9 C Set Cond Scale 100 Set cond scale 0 B 5 Menu overview Reference information B Telephone Service Q1p24cczonn4 Contract Number I Calibrate pH Calibr pH Buffer 1 Calibr pH Buffer 2 Calibrated Electrode Set pH Analogue Out Set pH Full Scale pH12 00 Set pH Zero Level pH2 00 Check Check Monitor Cell and Electa Check Service Mode Setup Setup Conductivity Setup p
30. n of damage is detected When required Clean the conductivity cell Clean the pH electrode 4 2 Cleaning the flow cell Remove the pH electrode and install the dummy electrode in the pH flow cell Pump a cleaning or sanitizing agent through the flow cells The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer WARNING NaOH is injurious to health Avoid spillage and use protective clothing and equipment Monitor pH C 900 User Manual 18 1120 06 Edition AF Maintenance 4 4 3 Cleaning the pH electrode Note The pH electrode has a limited life length and should be replaced every six months or when the response time is slow WARNING Always use protective equipment when handling dangerous chemicals Use one of the following procedures to clean the electrode to improve the response e Salt deposits Dissolve the deposit by immersing the electrode first in 0 1 M HCl then in 0 1 M NaOH and again in 0 1 M HCI Each immersion is for a 5 minute period Rinse electrode tip in distilled water between each solution e Oil or Grease Films Wash electrode tip in a liquid detergent and water If film is known to be soluble in a particular organic solvent wash with this solvent Rinse electrode tip in distilled water e Protein deposits Dissolve the deposit by immersing the electrode in a 1 pepsin solution in 0 1 M HCl for five minutes followed by thorough rinsing with di
31. ng 5 Fault Action 5 Clean the flow cell according to procedure in section 4 5 Cleaning the conductivity flow cell Conductivity measurement with the 1 Clean the flow cell according to procedure in same buffer appears to decrease section 4 5 Cleaning the conductivity flow cell over time 2 The ambient temperature may have decreased Use a temperature compensation factor see section B 2 1 Setting up conductivity temperature compensation in Reference information Absolute conductivity value wrong 1 Turn the flow cell so the end with the screws is facing the pH flow cell 2 Recalibrate the conductivity cell 3 Calibration solution 1 00 M NaCl not correctly prepared Prepare a new calibration solution and recalibrate the conductivity cell Ghost peaks appear the in gradient 1 A charged sample has been detected e g profile protein 2 Air bubbles are passing through the flow cell Check for loose tubing connections If necessary use a flow restrictor after the conductivity flow cell 5 2 3 Other problems Fault Action Error in external chart recorder 1 Check the chart recorder in accordance with its manual 2 Test the recorder function and input voltage which should be 1 V full scale 3 Check the conductivity scaling and pH scaling see menus Set cond scale 100 section 3 5 Set cond scale 0 section 3 5 and Using an external chart recorder section 3 8 No text on the front display 1 Check that the mai
32. ns cable is connected and the power switch is in ON position Monitor pH C 900 User Manual 18 1120 06 Edition AF 25 5 Trouble shooting 26 5 3 Error messages If the suggested actions do not correct the fault call GE Healthcare Message Action Cell constant is out of range 1 Wrong solution used during calibration Use 1 00 M NaCl and recalibrate 2 Air in the conductivity cell during calibration Flush the flow cell with calibration solution and recalibrate 3 Dirty conductivity flow cell Clean the cell according to section 4 5 Recalibrate the cell 4 Bad cell Replace pH diff is too small The difference between the zero level and full scale must be at least 1 pH unit Bad pH cell or not connected 1 Check that the pH electrode is connected 2 Replace the pH electrode Temp cell is bad or not connected 1 Check that the conductivity cell is connected Recalibrate 2 The calibration value differs from the predefined calibration value by more than 5 C Recalibrate If the message is still displayed replace the conductivity cell Too close between buffer 1 amp 2 The difference between the pH of the buffers used during calibration must be at least 1 pH unit Cal failed Very bad slope lt 10 or gt 199 Recalibrate If the message is still displayed replace the pH electrode Bad Slope Slope is lt 70 or gt 110 Clean the pH electrode and recalibrate If the messag
33. oaxial ceramic reference junction The flow cell should not be used with any other pH electrode A 3 Conductivity cell The flow cell has two cylindrical titanium electrodes positioned in the flow path of the cell An alternating voltage is applied between the electrodes and the resulting current is measured and used to calculate the conductivity of the eluent The monitor controls the AC frequency and increases it with increasing conductivity between 50 Hz and 50 kHz giving maximum linearity and true conductivity values Monitor pH C 900 User Manual 18 1120 06 Edition AF Reference information A The conductivity is automatically calculated by multiplying the measured conductance by the flow cell s cell constant The cell constant is pre calibrated on delivery but can be measured with a separate calibration procedure This procedure is described in Reference information section B 2 Temperature sensor Eluent gt One of the electrodes has a small temperature sensor for measuring the temperature of the eluent in the flow cell Temperature variations influence the conductivity and in some applications when highly precise conductivity values are required it is possible to program a temperature compensation factor that recalculates the conductivity to a set reference temperature Monitor pH C 900 User Manual 18 1120 06 Edition AF 29 B Reference information B Menus Check Monitor Cell and Electrode
34. onductivity flow cell on cccsssssssccccececceeseessssssssssnsssnenneeee 22 4 6 Changing conductivity flow cells oe sscssesseccccecceceeceecsssssnsssssnsssnneeeeese 22 4 7 Instrument housing oaececscsscsccscssssssssssssssssssssnsusssssssseseccseseceseeeeeeesesssssssssssassususssseseees 22 Monitor pH C 900 User Manual 18 1120 06 Edition AF Contents 5 Trouble shooting sicssssisisssscssicteessecestsescdevesberssedersdelaratasiie 23 Bee G n rdlliss nest inner te rame ee Rule cit 23 5 2 FOUS SANAGA NS Re nn RO A ed te 23 5 3 Error messages 6 Description nm nn Ee 27 6 1 ASUMEN sees ceca cence rer nn catia de re Maas ihe 27 6 2 PH ClCCHOUG sen orate eer dans cee O era gett etree toch 28 6 3 Conductivity cell 28 Ts MenUS a seas acess nanas 30 TI CHECKEN e ee tee acne 30 2 YS UPDIM NUS RER SR a e cas ie cht Ne hte AR 31 7 3 Setting and using the alarm timer ecceceecceccccssccssssssessssssssssssssssusssssesecseceesesceeeees 36 FA Service displays sets en A ane Rent 36 TS MIMENU OVERVIOW isn le nn ea n OR NENANA 37 8 Technical Specifications sssssscsssssssseseseseeseeeeseseees 38 9 Accessories and spare parts 41 10 Short instructions EL RE 42 Monitor pH C 900 User Manual 18 1120 06 Edition AF 5 1 Introduction 1 Introduction gt gt 1 1 General Monitor pH C 900 is a high precision on line monitor for measurement of pH and conductivity in liquid chromatography The pH C 900 features e Fast respons
35. ormation 40 Physical data EMC standards This device meets the requirements of the EMC Directive 89 336 EEC and other international requirements through the following harmonized standards e EN 61326 emission and immunity e EN 55011 GR 2 Class A emission e This device complies with part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference recevide including interference that may cause undesired operation Monitor pH C 900 User Manual 18 1120 06 Edition AF Reference information D D Accessories and spare parts Item Quantity Code no per pack Monitor pH C 900 without pH electrode and flow cells 1 18 1107 76 pH electrode round tip 1 18 1111 26 pH electrode with flow cell and holder round tip 1 18 1134 84 pH flow cell incl dummy electrode 1 18 1112 92 Dummy electrode round tip 1 18 1111 03 Conductivity flow cell 1 18 1111 05 1 3 Signal cable mini DIN open 18 1110 64 Teflon tubing i d 1 8 o d 3 16 m 18 1112 47 Tubing connector for 3 16 o d tubing 10 18 1112 49 Ferrule for 3 16 tubing 10 18 1112 48 Stop plug 5 16 5 18 1112 50 Stop plug 1 16 5 18 1112 52 Union Luer female 1 16 male 2 18 1112 51 Union 1 16 female M6 male 6 18 1112 57 Union M6 female 1 16 male 8 18 1112 58 PEEK tubing i d 0 75 mm o d 1 16 2m 18 1112 53 Teflon tubing i d 0 75 mm
36. rse the electrode in the List standard buffer solution normally pH 7 0 2 Select main menu Calibrate pH The display shows the current low and high calibrated pH value Press OK 3 Select sub menu Calib pH Buffer 1 press OK When the pH value has stabilized the Please wait message will disappear 4 Adjust the pH value in the display using the dial so that it corresponds to the known pH value of the 1 st buffer solution press OK The sub menu Calib pH Buffer 2 is shown 5 Rinse the electrode tip with distilled water and then immerse the electrode in the 2 nd standard buffer solution e g 4 0 or 9 0 press OK 6 When the pH value has stabilized the Please wait message will disappear 7 Adjust the pH value in the display using the dial so that it corresponds to the known pH value of the 2 nd buffer solution press OK 8 The sub menu Calibrated Electrode Slope shows the slope of the calibration curve where 100 corresponds to 59 16 mV per pH step at 25 C The asymmetry potential at pH 7 is shown as a mV value Press ESC to return to the main menu Voltage mV 500 mV Asymmetry potential at pH 7 500 mV 9 Before use rinse the electrode using distilled water A new electrode has a slope of typically 95 102 and an asymmetry potential within 30 mV As the electrode ages the slope decreases and the asymmetry potential increases Monitor pH C 900 User Manual 18 1120 06 Edition AF 17 3 Operation
37. sssasnees 10 2 9 Connecting to chart recorder if used oe eeeessssssssssssseennnnnesssssssseeeeeeeeeeeet 10 2 10 Connecting to communication LINK oe cccsssesssscccceceeceeeesessssssssssssssneneeeees 11 2 11 Connecting to supply voltage o ceeeesssssssssssssstessssscsssesssesseceeesessessssnsssssssssnsises 11 2 12 Preparing the instrument for USE eeeeesesesssssssssssessssesesssesmmetensnnssessssseseeeeeeeeeeet 12 ODER GEO assessors EA 13 ST ORO Re 13 3 2 Menu selection and settings o ceessssssssssssssssssssssssessscsecsesseceeceeceesessssssstsssnssssnanees 13 3 3 MAIN Men OVE EW oaae esea rE araea AEE A Aai 15 3 4 Reading pH and conductivity values ccccccssssssscccecceceseesesssssssssnsssnneeees 15 3 5 Setting conductivity scale oee cccceccccsssssssssssssssssssesssssssceseesssseseeseeceesesssssssnsssnssnssaeeee 15 36 Calibrating PH sirana eenaa A 16 3 7 Calibrating conductivity 18 3 8 Using an external chart recorder eeessssssssssssssssssesssnnssssscccessesesseeseeeeeeeeee 18 3 9 Storage and shut down eesesesesessesssssscccecsssecceccesesssessstseeeesssssssseeeeeeseseeeeeceeeeeneeee 19 3 10 Restart after power failure oeeeesesesseesssssssssscssesccccecsesesessensnsssssscceseesesssececeeeeesee 19 MOIR ENANCE san ii die sise 20 GET Periodic mainte Na NE hisser nn nier 20 4 2 Cleaning the flow cell een 20 4 3 Cleganing the pH electrode sn nn es ce 21 44 CHANGING pH lectrode 2 48 attirant 21 4 5 Cleaning the c
38. stilled water If these procedures fail to rejuvenate the electrode the problem is most likely a clogged liquid junction 1 Heat a 1 M KNO solution to 60 80 C 2 Place the electrode tip in the heated KNO solution 3 Allow the electrode to cool while immersed in the KNO solution before re testing If these steps fail to improve the electrode response replace the electrode 4 4 Changing pH electrode See section 2 4 Installing the pH electrode Monitor pH C 900 User Manual 18 1120 06 Edition AF 21 4 Maintenance A 22 4 5 Cleaning the conductivity flow cell WARNING When using hazardous chemicals make sure that the entire system has been flushed thoroughly with bacteriostatic solution e g NaOH and distilled water before service and maintenance If the conductivity measurements are not comparable to previous results the electrodes in the flow cell may be contaminated and require cleaning To clean the flow cell 1 Pump 15 ml of 1 M NaOH at 1 ml min through the flow cell either by using a pump or a syringe 2 Leave it for 15 minutes 3 Rinse thoroughly with 50 ml de ionized water Note If the flow cell is totally blocked the blockage can be broken using a thin needle or a piece of string with a diameter less than 0 8 mm 4 6 Changing conductivity flow cells The flow cells can be changed when required Make sure the instrument is switched off before disconnecting connecting the cells to the r
39. the monitor are 0 1 V There is also a 4 20 mA signal available at the same output connectors For the conductivity signal 0 represents 0 V and 100 represents 1 V as set under main menus Set Cond scale 0 and Set Cond scale 100 For the pH signal the full scale and zero level has to be set according to below 1 Select main menu Set pH Analogue Out press OK 2 Select sub menu Set pH Full Scale Set the range value press OK The range is the full scale pH range for the chart recorder 1 V 3 Select sub menu Set pH Zero Level press OK 4 Setthe value press OK The zero level is the pH value corresponding to 0 V to the chart recorder The difference between zero level and full scale must be at least 1 pH unit Monitor pH C 900 User Manual 18 1120 06 Edition AF Operation 3 3 9 Storage and shut down CAUTION Never leave the pH electrode in the flow cell for any period of time when the system is not used since this may cause the glass membrane of the electrode to dry out Dismount the pH electrode from the flow cell and fit the end cover filled with a 1 1 mixture of pH 4 buffer and 1 M KNOs Do NOT store in water only 3 9 1 Storage of conductivity flow cell Overnight The conductivity cell can be left filled with a buffer Weekend or Long time storage Flush the conductivity cell with water and fill with 20 ethanol 3 9 2 Storage of pH electrode The pH electrode should always be stored in a 1 1 mixture of p
40. tion by pressing the ESC button repeatedly Alternative display of conductivity with horizontal bar graph Setting conductivity full scale Setting conductivity zero point Calibration of pH monitor Setting the recorder output for the pH measurement Check internal operating values See Reference information section B 1 Setup conductivity pH temp language etc See Reference information section B 2 Set different timer option See Reference information section B 3 3 4 Reading pH and conductivity values The main operating menu shows the conductivity as a percentage of full scale together with the current temperature in the flow cell and the pH value If the pH value is not stable or is changing an asterisk is displayed after the value e g 4 02 The menu is reached from any other menu by pressing the ESC button repeatedly If temperature compensation is switched on the display will show CondTc instead of Cond see menu Setup conductivity temperature compensation in section B 2 1 of Reference information By turning the dial one click clockwise an alternative display of the conductivity is shown This display shows the actual conductivity value in mS cm together with the percentage value and as a horizontal bar graph with 10 resolution If temperature compensation is switched on Te is shown in the display The display of pH and temperature can be disabled see menu etup pH display in section B 2 5 and Setup temperature
41. to a grounded mains socket The instrument contains no user replaceable fuse Monitor pH C 900 User Manual 18 1120 06 Edition AF 11 2 Installation 12 2 10 Preparing the instrument for use Before the instrument is ready to use e Set the conductivity cell constant see section B 2 3 e Calibrate the temperature sensor see section B 2 6 e Calibrate the pH electrode see section 3 6 Note The conductivity cell constant is shown on the packaging Retain the packaging in case the conductivity cell constant needs to be re entered Note Measured temperature is the temperature in the conductivity flow cell which can differ from the ambient temperature Note When running chromatography using organic solvents it is recommended that the pH electrode is removed and the dummy electrode inserted in its place as organic solvents will cause pH electrode degeneration Before performing these procedures you are recommended to read sections 3 1 3 3 WARNING The computer should be installed and used according to the instructions provided by the manufacturer of the computer Monitor pH C 900 User Manual 18 1120 06 Edition AF Operation 3 3 Operation A Selftest Monitor pH C900 Calibrating Calibration OK Cond Temp pH 254 22 9 C 11 50 WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and glov
42. vity Environment 1 uS cm to 999 9 mS cm Max 2 of full scale calibrated range or 10 uS cm whichever is greater in the range 1 uS cm to 300 mS cm Max 1 or 5 uS cm Max 3 or 15 uS cm Max 0 5 of full scale calibrated range Max 3 s 0 95 of step 2 0 C 0 5 C per 10h 1 within 0 100 ml min 4 to 40 C 20 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure Flow cells pH cell Max Flow rate Max Pressure Back pressure Internal volume Wetted materials Chemical resistance 38 100 ml min 0 5 MPa 5 bar 72 psi Max 0 02 MPa 0 2 bar 2 9 psi 88 ul pH electrode and flow cell Glass FFKM perfluororubber titanium Dummy electrode PTFE polytetrafluoroethylene The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 Ethyl acetate 100 Hexane and 100 Tetrahydrofuran THF Monitor pH C 900 User Manual 18 1120 06 Edition AF Conductivity cell Reference information C Max Flow rate Max Pressure Back pressure Internal volume Wetted materials pH stability range Chemical resistance 100 ml min 5 MPa 50 bar 725 psi Max 0 01 MPa 0 1 bar 1 5 psi 14 ul Titanium CTFE 1 13 1 14 lt 1 days exposure The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 Ethylacetate 100 Hexane and 100 T
43. w cell and the rear panel of the monitor using a standard laboratory 4mm banana plug cable 11 Check that the pH electrode has been calibrated at the correct temperature 12 In organic solvents such as ethanol methanol and acetonitrile stable pH measurements are not possible since dehydration of the membrane will occur It is recommended that the pH electrode is not used in applications using organic solvents Mount the dummy electrode instead 13 Clogged liquid junction Refer to section 4 3 Cleaning the pH electrode pH values vary with varied 1 back pressure Replace the pH electrode 5 2 2 Conductivity measurement Fault Action Incorrect or unstable reading 1 Check that the conductivity flow cell cable is connected properly to the rear of the instrument Check that the pump and valves operate correctly If temperature compensation is being used check that the temperature sensor is calibrated and that the correct temperature compensation factor is in use Check that the column is equilibrated If necessary clean the column Check the operation of the mixer Baseline drift or noisy signal 1 There may be air in the flow cell Use a flow restrictor after the flow cell Check for leaking tubing connections Check the column is equilibrated If necessary clean the column Check the operation of the mixer and the pump Monitor pH C 900 User Manual 18 1120 06 Edition AF Trouble shooti

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