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Procise/Procise cLC Protein Sequencing Systems Site Prep and

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3. Applied Bivosystems gt Instrument Safety 4 13 Input Output Connections Location The locations of the input output connections on the Procise or Procise cLC Protein 4 14 Instrument Safety Sequencer are shown in the figure below The input output connections are also labeled on the instrument
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6. BH ME gt en a a gt 2 DF JE MIS Applied Biosystems E Instrument Safety 4 5 Symboles des alertes de s curit Symboles lectriques Le tableau suivant donne la signification de tous les symboles lectriques qui figurent sur les appareils Applied Biosystems En pr sence de l un de ces symboles il est imp ratif de se conformer aux consignes de s curit appropri es Position MARCHE de l interrupteur d alimentation principal Position ARR T de l interrupteur d alimentation principal Positions MARCHE ARRET de l interrupteur d alimentation principal bouton poussoir Borne pouvant tre r
7. Este s mbolo indica la posici n de encendido apagado de un interruptor principal de presi n Este s mbolo indica que existe la posibilidad de conectar esta terminal a la toma de tierra de referencia de otro instrumento Esta no es una toma de tierra protegida Este s mbolo indica que la toma de tierra protegida debe ser conectada a tierra antes de realizar cualquier otro tipo de conexion el ctrica al instrumento Una terminal marcada con este s mbolo recibe o suministra corriente o tensi n alterna Una terminal marcada con este s mbolo puede recibir o suministrar corriente o tensi n alterna y continua PRECAUCI N Este s mbolo indica la presencia de alta tensi n y advierte al usuario que proceda con precauci n Pbutbw oo PRECAUCI N Este s mbolo indica que consulte el manual para obtener m s informaci n y que proceda con precauci n S mbolos no A continuaci n se presenta un glosario ilustrado de todos los s mbolos de seguridad y el ctricos alerta no el ctricos que aparecen en los instrumentos de Applied Biosystems 4 12 Instrument Safety PRECAUCI N Este s mbolo indica peligro de altas temperaturas Proceda con cautela cuando trabaje cerca de estas zonas para evitar quemarse con componentes calientes gt gt Este s mbolo indica que hay un l ser dentro del instrumento
8. WARNING Risk of electric shock Disconnect power cord from supply before replacing fuses or removing power supply module from instrument Avertissement Risque de choc lectrique D lectrique D brancher le cordon d alimentation avant de remplacer les fusibles ou de retirer le block d alimentation de l instrument WARNING For continued protection against risk of fire replace only with Listed and Certified fuse of the specified type and ratings Avertissement Pour assurer une protection continue contre les risques d incendie remplacer les fusibles uniquement par des fusibles num r s et certifi s du type de courant nominal specifi s WARNING HOT LAMP Avertissement LAMPE CHAUDE WARNING HOT Replace lamp with an Applied Biosystems lamp Composants chauds Remplacer la lampe par une lampe Applied Biosystems WARNING Disconnect supply cord before opening Grounding circuit continuity is vital for safe operation of equipment Never operate equipment with grounding conductor disconnected Avertissement D brancher la corde d approvisionnement avant l ouverture La continuit de circuit au sol est essentiel pour l exploitation s re du mat riel N actionnez jamais le mat riel avec le conducteur debranch WARNING For protection against fire hazard replace only same type and rating of fuse Avertissement A fin d assurer la protection contre les risques d incendie remplacer un
9. gt Computer Pump communication Detector input GR0821 qu Figure 4 1 Input Output connections on the Procise and Procise cLC Protein Sequencer Gas Safety Pressurized Gas Safety Gaseous Waste Safety This instrument uses a pressurized gas cylinder for operation Some kinds of rapidly leaking gas can displace normal atmosphere and cause suffocation If knocked over a pressurized gas cylinder can explode Keep in mind the following When working with gas cylinders screw the gas cap on tightly when the cylinder is not in use and when you are transporting it Attach the cylinder firmly to a wall or gas cylinder cart by means of an approved strap or clamp NUNNE EXPLOSION HAZARD Pressurized gas cylinders are potentially explosive Always cap the gas cylinder when it is not in use and attach it firmly to the wall or gas cylinder cart with approved brackets or chains Instruments that use gas cylinders for operation emit gaseous waste that is potentially hazardous To handle gaseous waste safely in your laboratory perform the following steps Follow the instructions provided in Laboratory Ventilation Requirements on page 2 10 Read the MSDSs provided by the manufacturer of the chemicals used in your laboratory Operate the fume hood whenever the instrument power is on waste is in the waste container or reagents are on the instrument Vent according to th
10. Systems 1 800 899 5858 then press 15 1 508 383 7855 Peptide Synthesis Pioneer and 9050 Plus Peptide Synthesizers 1 800 899 5858 then press 15 1 508 383 7855 PNA Custom and Synthesis 1 800 899 5858 then press 15 1 508 383 7855 FMAT 8100 HTS System and Cytofluor 4000 Fluorescence Plate Reader 1 800 899 5858 then press 16 1 508 383 7855 Chemiluminescence Tropix 1 800 542 2369 U S only or 1 781 271 0045 1 781 275 8581 Applied Biosystems MDS Sciex 1 800 952 4716 1 650 638 6223 Outside North America Region Telephone Dial Fax Dial Africa and the Middle East Africa English Speaking and West Asia Fairlands South Africa 27 11 478 0411 27 11 478 0349 South Africa Johannesburg 27 11 478 0411 27 11 478 0349 Middle Eastern Countries and North Africa Monza Italia 39 0 39 8389 481 39 0 39 8389 493 Introduction 1 5 1 6 Introduction Region Telephone Dial Fax Dial Eastern Asia China Oceania Australia Scoresby Victoria 61 3 9730 8600 61 3 9730 8799 China Beijing 86 10 64106608 86 10 64106617 Hong Kong 852 2756 6928 852 2756 6968 Korea Seoul 82 2 593 6470 6471 82 2 593 6472 Malaysia Petaling Jaya 60 3 758 8268 60 3 754 9043 Singapore 65 896 2168 65 896 2147 Taiwan Taipei Hsien 886 2 22358 2838 886 2 2
11. are compatible with vented waste Do not simultaneously vent strong oxidizers bases or other chemicals that are incompatible with gaseous waste Maintain negative pressure in duct Waste line tubing or flexible duct Face open end of tubing away from incoming air Place tubing as far into the hood or canopy as possible Use a straight upward run of no more than 3 m 15 ft of polypropylene tubing Securely fasten tubing with polypropylene or Teflon fasteners not brass corrodes Operate laboratory ventilation system whenever the instrument power is on waste is in the waste bottle or reagents are on the instrument This includes nights and weekends Do not connect instrument to a ductless hood or a system that purifies filters air and returns it to the room Site Preparation 2 11 Laboratory Ventilation System Building air intake Fume hood Fume Hood Must not draw gaseous Verify that construction waste back into the materials are compatible building with vented waste Do not simultaneously vent strong oxidizers bases or other chemicals that are incompatible with gaseous waste Gaseous waste Locate hood away from line from air currents created by instrument air conditioning ducts fans windows doors or moving objects or people i that ocal state provincial or national standards Post label at sash level which provides an average face level air flow
12. performance Store the chemicals and reagents as indicated in the table below Read the MSDS sheets for safe handling of the items Note All reagents are common to both kits below P N 401690 and P N 402064 unless otherwise indicated For R2 R5 and PTH standards you may receive both or only one of each type Table 2 1 Procise or Procise cLC Protein Sequencer Installation Chemical Kit Components P N 401690 and P N 402064 Storage P N Description Conditions 400208 R1 5 PITC in N Heptane 20 C 401535 R2B n Methylpiperidine in methanol and water 4 C 4310689 R2C n Methylpiperidine in butanol isopropanol and water 4 C 400003 R3 Trifluoroacetic Acid Room temp 400028 RAA 25 Trifluoroacetic Acid in water 4 C 400315 R5 Acetonitrile with DTT Room temp 4340966 R5B Acetonitrile with n Acetylcysteine Room temp 400079 S1 N Heptane Procise cLC only Room temp 400854 S2B Ethyl Acetate Room temp 400008 S3 N Butyl Chloride Room temp 400314 S4B 20 Acetonitrile in water Procise only Room temp 400470 MeOH Methanol Room temp 400879 PTH Analyzer Standards 20 C 4340968 PTH AA standards solution 40mL bottle Room temp 400385 Biobrene Plus 4 C 400979 B Lactoglobulin 4 C 401446 Premix buffer concentrate 4 C 402051 S4C 10 Acetonitrile in water cLC Room temp 401464 Solvent A3 3 5 THF Tetrahydrofuran in Water 1 L Procise Room temp 401570 Solvent B2 12 Isopropanol and Acetonitrile 1 L Procise Room temp 401887 So
13. 3 velocity of 30 linear m min zi 100 linear ft min See Laboratory Ventilation Specifications on page 2 10 Fume hood Waste Line Tubing Use no more than 3 m 15 ft of polypropylene tubing Securely fasten tubing with polypropylene or Teflon fasteners not brass corrodes GR1743b Ventilation Operation Operate laboratory ventilation system whenever the instrument power is on waste is in the waste bottle or reagents are on the instrument This includes nights and weekends Do not connect instrument to a ductless hood or a system that purifies filters air and returns itto the room Fume Hood Follow these guidelines for fume hood operation and maintenance Operation 4 Operate the fume hood whenever the instrument power is on waste is in the waste container or reagents are on the instrument Use a fume hood that is constructed of materials that are compatible with the waste materials chemicals being generated or exhausted Locate the fume hood away from air currents generated by air conditioning ducts fans windows doors and moving equipment and persons Locate the fume hood exhaust vent where gaseous waste cannot be drawn back into the building 2 12 Site Preparation Waste Ventilation Warnings Affix a sign or label to indicate the position of the fume hood sash which produces an average airflow of 100 linear ft min face velocity The minimum flow velocity at any point
14. 5 Safety Alert Symbols French 4 6 Safety Alert Symbols German 4 7 Safety Alert Symbols Italian eeeoe es 4 8 Safety Alert Symbols Japanese 4 9 Safety Alert Symbols Korean 4 10 Safety Alert Symbols Portuguese 4 11 Safety Alert Symbols Spanish 4 12 Safety Alert Symbols Thai 2 2222 eos 4 13 Input Output Connections 4 14 Gas Safety rar uoces ee ito ee ECR EA 4 15 A Acronyms and Abbreviations Acronyms and Abbreviations Used in MSDSS A 1 Introduction Overview About the Site Preparation and Safety Guide In This Guide The Site Preparation and Safety Guide is sent to all customers who have purchased an Applied Biosystems instrument This guide provides the information you need to fully prepare your site for the arrival and installation of the instrument Complete preparation helps ensure a smooth installation process as well as correct and safe instrument operation This guide for the Procise and Procise cLC Protein Sequencer contains the following chapters and appendix Chapter Title Description Introduction Pro
15. ESAS UEH LC 0 XE A 2 VF Es Age BOL SY SELLE 0 72157 XE Mae na AR AUS WI see USLICI F9 0 WS Deol ees UHUH FOIE AS DUC 9 Ol ASE O ANNE SHS 27 Sol SSNS ADS AS Sel FAS As e gasu a 417 E Applied Biosystems 212101M BASE Hl 217 CHA 2100 coli CS9 7 Ho FA Ol Se 218 ABS LIEIBILILE O SHAN ASE WE LA o 9 SAS TIS Sol 0 AS MO 2121010 4 10 Instrument Safety S mbolos de alerta de seguranca S mbolos eletricos Atabela a seguir constitui um gloss rio ilustrado de todos os s mbolos el tricos usados nos instrumentos Applied Biosystems Sempre que um desses s mbolos aparecer num instrumento siga os procedimentos adequados de seguran a Este s mbolo indica que o interruptor de energia el trica est na posi o ligado Este s mbolo indica que o interruptor de energia el trica est na posi o desligado Este s mbolo indica a posi o ligado desligado de um interruptor principal de energia el trica do tipo bot o de press o Este s mbolo indica que um terminal pode estar conectado a uma refer ncia de aterramento de sinal de um outro instrumento Este nao um terminal terra protegido Este s mbolo indica que este um terminal de aterramento
16. Laboratory Use Part 1 General Requirements This instrument has been tested to and complies with ICES 001 Issue 3 Industrial Scientific and Medical Radio Frequency Generators Safety This instrument meets European requirements for safety Low Voltage Directive 73 23 EEC This instrument has been tested to and complies with standards EN 61010 1 2001 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements and EN 61010 2 010 Particular Requirements for Laboratory Equipment for the Heating of Materials EMC This instrument meets European requirements for emission and immunity EMC Directive 89 336 EEC This instrument has been tested to and complies with standard EN 61326 Group 1 Class B Electrical Equipment for Measurement Control and Laboratory Use EMC Requirements AustralianEMC Standards C This instrument has been tested to and complies with standard AS NZS 2004 Limits and Methods Measurement of Electromagnetic Disturbance Characteristics of Industrial Scientific and Medical ISM Radio frequency Equipment Electrical Requirements Power Power Line Electrical Outlets Power Rating Power Cords Grounding IMPORTANT You must be able to disconnect the main power supply to the instrument immediately if necessary The following table specifies the electrical operating range for the instrument in various parts of the wo
17. Supplies Before the Procise or Procise cLC Protein Sequencer is installed the installation site must be prepared so that the instrument can be operated correctly and safely Follow the requirements presented in Preinstallation Checklists on page 2 2 to simplify the installation procedure Training of operators is one of the primary goals of installation Personnel requiring training should set aside 2 uninterrupted days to work with the Applied Biosystems service representative If this is not possible please call Applied Biosystems Service Administration to reschedule the installation Optimization of the instrument and verification of performance will be performed by an Applied Biosystems service representative during the installation Before installation be sure to contact Sales Administration or your sales representative to order the additional supplies necessary for the ongoing operation of the instrument The chemicals shipped with this instrument will be completely consumed during the installation and initial testing of the instrument Site Preparation 2 1 Preinstallation Checklists About These Use the following preinstallation checklists to ensure that all preparations are made for Checklists installing your instrument All of the personnel and supplies on these checklists are required at the time of installation but most of them are not supplied by Applied Biosystems A service representative will contact you to confi
18. appliedbiosystems com techsupp b Click the Index link for the document type you want then find the document you want and record the index number c Use the index number when requesting documents following the procedures below by phone for fax a From the U S or Canada call 1 800 487 6809 or delivery from outside the U S and Canada call 1 858 712 0317 b Follow the voice instructions to order the documents you want Note There is a limit of five documents per request through the Internet for fax or e mail delivery a Access the Applied Biosystems Technical Support Web site at http www appliedbiosystems com techsupp Under Resource Libraries click the type of document you want Enter or select the requested information in the displayed form then click Search In the displayed search results select a check box for the method of delivery for each document that matches your criteria then click Deliver Selected Documents Now or click the PDF icon for the document to download it immediately Fill in the information form if you have not previously done so then click Deliver Selected Documents Now to submit your order Note There is a limit of five documents per request for fax delivery but no limit on the number of documents you can order for e mail delivery Introduction 1 7 Site Preparation Before You Start Preinstallation Operator Training Performance Verification Ordering
19. in the hood is 80 linear ft min and the maximum is 125 linear ft min Ensure that the fume hood meets all local state provincial or national safety requirements Have a safety professional or mechanical ventilation expert check and record air velocity a minimum of once a year Inspect and maintain the exhaust system including fans and motors a minimum of once a year PINZE CHEMICAL WASTE HAZARD Waste produced by Applied Biosystems instruments can be hazardous and can cause injury illness or death Operate a vented instrument only if it is connected in accordance with all requirements Operate the system venting gaseous waste whenever the instrument power is on waste is in the waste container or reagents are on the instrument Handle all liquid solid and gaseous waste as potentially hazardous See the MSDSs provided by the manufacturer s of chemicals used at your site for more information Connect the exhaust line so that it leads to the fume hood or duct in a straight and upward direction Low points will allow condensation to collect preventing flow through the instrument Do not connect the waste vent line to a ductless hood or to a system that purifies and filters air and returns it to the room Always mix and prepare hazardous materials beneath an operating fume hood Dispose of waste in accordance with all local state provincial or national health and safety regulations and laws Venting hazardous wa
20. install it PHYSICAL INJURY HAZARD Do not attempt to lift the instrument or any other heavy objects unless you have received related training Incorrect lifting can cause painful and sometimes permanent back injury Use proper lifting techniques when lifting or moving the instrument Two or three people are required to lift the instrument depending upon instrument weight A typical laboratory layout is shown below Power Color monitor E ar 5 Solvent O PROCISE and 490 PROCISE Protein Sequencer PTH Waste System Bottles Keyboard Mouse and mouse pad p E e xs r3 E E el 2 8 x s pr 5 a GR1707 Site Preparation 2 9 Laboratory Ventilation Requirements Laboratory Ventilation Specifications About the Waste Exhaust System Connecting the Gaseous Waste Exhaust Line Connecting the Fluid Waste Line Connecting the Flexible Duct 2 10 Site Preparation The laboratory ventilation system s to this instrument must be operating properly whenever the instrument power is on waste is in the waste container or reagents are on the instrument For the Procise and Procise cLC Protein Sequencer proper ventilation operation specifications include 4 A duct or fume hood with an average of 30 linear m min 100 linear feet min face leve
21. of chemical waste Provide primary and secondary waste containers A primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste material and meet federal state and local requirements for container storage Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional safety guidelines consult the MSDS Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the MSDS Handle chemical wastes in a fume hood After emptying the waste container seal it with the cap provided Dispose of the contents of the waste tray and waste bottle in accordance with good laboratory practices and local state provincial or national environmental and health regulations The waste for this instrument is collected in a 1 L 26 gallon glass container Dispose of the waste when the waste bottle is 75 full The composition of the chemical waste may vary depending upon the protocols used the number of samples the volumes specified and the reagents included in the protocols When handling chemical waste we strongly recommend that you Read the waste profile s in this chapter before handling o
22. reagent bottles from becoming overpressurized and possibly exploding the pressure regulator and the pressure relief valve must be in place and working properly Failure of these two components will cause bottle overpressurization and bottle explosion Attach the pressurized gas cylinder firmly to a wall or gas cylinder cart by means of approved straps or clamps VN he EXPLOSION HAZARD Pressurized gas cylinders are potentially explosive Always cap the gas cylinder when it is not in use and attach it firmly to the wall or gas cylinder cart with approved brackets chains or clamps Site Preparation 2 17 Chemical Safety Overview In This Chapter This chapter contains general information about handling hazardous chemicals and waste It also contains information about Material safety data sheets MSDSs 4 Hazardous waste produced if any during the installation procedure Hazardous waste produced during typical use of the instrument if appropriate Applied Biosystems assumes that all operations in your laboratory will be conducted in accordance with safety practices detailed in the MSDSs for the chemicals used in your laboratory and with all local state provincial or national regulations Chemical Safety 3 1 Material Safety Data Sheets Overview About MSDSs Updating MSDSs Ordering MSDSs from Applied Biosystems 3 2 Chemical Safety Some of the chemicals used with this instrument may be listed as hazardous by thei
23. waste with a minimum draw of 2 inches of H5O Provided a system for instrument ventilation with a minimum draw of 30 linear m min 100 linear ft min Ensured that instrument ventilation and exhaust systems to outside air will meet all local state provincial or national air quality regulations Obtained permit if necessary Met other laboratory ventilation requirements as specified in Laboratory Ventilation Specifications on page 2 10 Established proper handling and disposal method s for hazardous chemical waste if appropriate Verified that room ventilation can accommodate instrument heat output of 5690 Btu h 1 66 kW Verified that laboratory ventilation can accommodate other environmental requirements as specified in Laboratory Environmental Requirements on page 2 14 Electrical A dedicated 2 0 kVA power line and ground is required for the system A line conditioner or UPS on the power line is recommended At least 2 standard power outlets are within 2 5 m 8 ft of the instrument location preferably near the back of the instrument Separate power outlets on the same dedicated line are recommended for the computer monitor and optional printer Met requirements specified in Electrical Requirements on page 2 15 Site Preparation 2 3 Equipment and You must supply the following items for installation Check off each item as you verify Consu
24. 358 2839 Thailand Bangkok 66 2 719 6405 66 2 319 9788 Europe Austria Wien 43 0 1 867 35 75 0 43 0 1 867 35 75 11 Belgium 32 0 2 712 5555 32 0 2 712 5516 Czech Republic and Slovakia Praha 420 2 61 222 164 420 2 61 222 168 Denmark Naerum 45 45 58 60 00 45 45 58 60 01 Finland Espoo 358 0 9 251 24 250 358 0 9 251 24 243 France Paris 33 0 1 69 59 85 85 33 0 1 69 59 85 00 Germany Weiterstadt 49 0 6150 101 0 49 0 6150 101 101 Hungary Budapest 36 0 1 270 8398 36 0 1 270 8288 Italy Milano 39 0 39 83891 39 0 39 838 9492 Norway Oslo 47 23 12 06 05 47 23 12 05 75 Poland Lithuania Latvia and Estonia 48 22 866 40 10 48 22 866 40 20 Warszawa Portugal Lisboa 351 0 22 605 33 14 351 0 22 605 33 15 Russia Moskva 7 095 935 8888 7 095 564 8787 South East Europe Zagreb Croatia 385 1 34 91 927 385 1 34 91 840 Spain Tres Cantos 34 0 91 806 1210 34 0 91 806 1206 Sweden Stockholm 46 0 8 619 4400 46 0 8 619 4401 Switzerland Rotkreuz 41 0 41 799 7777 41 0 41 790 0676 The Netherlands Nieuwerkerk a d IJssel 31 0 180 331400 31 0 180 331409 United Kingdom Warrington Cheshire 44 0 1925 825650 44 0 1925 282502 All other countries not listed 44 0 1925 282481 44 0 1925 282509 Warrington UK Japan Japan Hacchobori Chuo Ku Tokyo 81 3 5566 6230 81 3 5566 650
25. 7 Latin America Del A Obregon Mexico 305 670 4350 305 670 4349 To Reach Technical Westrongly encourage you to visit our Web site for answers to frequently asked Support Through questions and for more information about our products You can also order technical the Internet documents or an index of available documents and have them faxed or e mailed to you through our site The Applied Biosystems Web site address is http www appliedbiosystems com support To submit technical questions from North America or Europe Step Action 1 Access the Applied Biosystems Technical Support Web site 2 Under the Troubleshooting heading click Support Request Forms then select the relevant support region for the product area of interest 3 Enter the requested information and your question in the displayed form then click Ask Us RIGHT NOW blue button with yellow text 4 Enter the required information in the next form if you have not already done so then click Ask Us RIGHT NOW You will receive an e mail reply to your question from one of our technical experts within 24 to 48 hours To Obtain Free 24 hour access to Applied Biosystems technical documents including MSDSs is available by fax or e mail or by download from our Web site Documents on Demand To order documents Then by index number a Access the Applied Biosystems Technical Support Web site at http www
26. Procise Procise cLC Protein Sequencing Systems Site Preparation and Safety Guide Applied AS Bibsystems Copyright 2000 2002 2003 2004 Applied Biosystems All rights reserved For Research Use Only Not for use in diagnostic procedures Information in this document is subject to change without notice Applied Biosystems assumes no responsibility for any errors that may appear in this document This document is believed to be complete and accurate at the time of publication In no event shall Applied Biosystems be liable for incidental special multiple or consequential damages in connection with or arising from the use of this document TRADEMARKS Applied Biosystems ABI PRISM BioCAD BioLIMS BioMerge CytoFluor FMAT GeneScan Poros and Procise are registered trademarks of Applera Corporation or its subsidiaries in the U S and or certain other countries AB Design Applera Expedite Mariner SQL GT and Voyager are trademarks of Applera Corporation or its subsidiaries in the U S and or certain other countries MDS and Sciex are registered trademarks of MDS Inc Applied Biosystems MDS Sciex Instruments is a joint venture between Applera Corporation and MDS Inc Microsoft and Windows are registered trademarks of Microsoft Corporation All other trademarks are the sole property of their respective owners Part Number 4314377 Rev C 04 2004 Contents Introduction OVELVIEW 2 2 Dida hic 1 1 About Prems
27. SDSs before using reagents or solvents As the generator of potentially hazardous waste it is your responsibility to perform the actions listed below Characterize by analysis if necessary the waste generated by the particular applications reagents and substrates used in your laboratory Ensure the health and safety of all personnel in your laboratory Ensure that the instrument waste is stored transferred transported and disposed of according to all local state provincial or national regulations Note Radioactive or biohazardous materials may require special handling and disposal limitations may apply Chemical Hazard PNE CHEMICAL HAZARD Some of the chemicals used with Applied Biosystems Warning instruments are potentially hazardous and can cause injury illness or death Read and understand the material safety data sheets MSDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials Minimize contact with and inhalation of chemicals Wear appropriate personal protective equipment when handling chemicals e g safety glasses gloves or protective clothing For additional safety guidelines consult the MSDS Do not leave chemical containers open Use only with adequate ventilation Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended on the MSDS Comply with all local s
28. Symbol zeigt die EIN Position des Hauptnetzschalters an Dieses Symbol zeigt die AUS Position des Hauptnetzschalters an Dieses Symbol zeigt die EIN AUS Position eines Druck Zug Hauptnetzschalters an Dieses Symbol zeigt an da ein Anschlu an die Betriebserde eines anderen Instruments angeschlossen werden kann Dies ist kein gesch tzter ErdanschluB Dieses Symbol zeigt einen gesch tzten Erdanschlu an der geerdet werden 3 bevor andere elektrische Anschl sse zum Instrument hergestellt werden Ein mit diesem Symbol gekennzeichneter Anschlu kann Wechselstrom oder spannung erhalten oder abgeben Ein mit diesem Symbol gekennzeichneter Anschlu kann Wechselstrom oder spannung und Gleichstrom oder spannung erhalten oder abgeben VORSICHT Dieses Symbol zeigt das Vorliegen von Hochspannung an und warnt den Anwender mit Vorsicht fortzufahren gt gt Dr VORSICHT Dieses Symbol fordert Sie auf das Handbuch zwecks n herer Informationen zu konsultieren und mit Vorsicht fortzufahren Nicht elektrische Die folgende Tabelle enth lt Beschreibungen aller auf den Instrumenten von Symbole Applied Biosystems verwendeten nicht elektrischen Symbole VORSICHT Dieses Symbol zeigt eine Gefahr durch die Heizung an Gehen Sie mit Vorsicht vor wenn Sie in der N he dieser Bereiche arbeiten damit Sie sich nicht an hei en Komponenten verbrennen gt gt Dieses Symbol
29. cal Contact technical support by e mail for help in the following product areas Support by E Mail Hours for Telephone Technical Support To Contact Technical Support by Telephone or Fax 1 4 Introduction Product Area E mail address Genetic Analysis DNA Sequencing galab appliedbiosystems com Sequence Detection Systems and PCR pcrlab O appliedbiosystems com Protein Sequencing Peptide and DNA Synthesis corelab appliedbiosystems com Biochromatography PerSeptive DNA PNA and Peptide Synthesis systems CytoFluor FMAT Voyager and Mariner Mass Spectrometers tsupport O appliedbiosystems com LC MS Applied Biosystems MDS Sciex apisupport O sciex com or api3 support O sciex com Chemiluminescence Tropix tropix appliedbiosystems com In the United States and Canada technical support is available at the following times Product Hours Chemiluminescence 8 30 a m to 5 30 p m Eastern Time Framingham support 8 00 a m to 6 00 p m Eastern Time All Other Products 5 30 a m to 5 00 p m Pacific Time In North America To contact Applied Biosystems Technical Support use the telephone or fax numbers given below To open a service call for other support needs or in case of an emergency dial 1 800 831 6844 and press 1 Product or Product Area Fax Dial Telephone Dial ABI PRISM 3700 DNA Analyze
30. cal kit and store the components as specified in Table 2 1 on page 2 6 Do not move or unpack instrument cartons This protects you from liability if any damage occurred during shipping Inspect instrument cartons and report any damage to your Applied Biosystems service representative PHYSICAL INJURY HAZARD Do not move or unpack instrument cartons This instrument is heavy Any incorrect lifting or moving of the instrument can cause painful and sometimes permanent back injury The instrument may tip over if moved or unpacked incorrectly causing serious injury to persons in its path or damage to the instrument itself Unpacking the instrument will also void your warranty with Applied Biosystems You must unpack the installation chemistry kit which is boxed separately from the instrument Read the MSDSs supplied with the chemicals and store the components as specified on page 2 6 CHEMICAL HAZARD Some chemicals used with Applied Biosystems instruments are hazardous and can cause injury illness or death Always read the appropriate MSDSs before interacting with the instrument and chemicals in any way Hazardous Chemical Warnings are prominently displayed on the labels of all hazardous materials Site Preparation 2 5 Installation Chemistry Kit 2 6 Site Preparation Unpack the Procise and Procise cLC installation chemical kit upon receipt The installation chemical kit is intended to be used during installation to verify instrument
31. ction Association publishes recommended regulations for local or state governments in the United States hazardous rating system developed by this association NIOSH National Institute of Occupational Safety and Health USA recommends exposure levels and respirators oc Open cup testing for flash point Acronyms and Abbreviations A 1 Term Explanation OSHA Occupational Safety and Health Administration USA sets chemical exposure levels PEL Permissible exposure limit the federal OSHA limit usually expressed as time weighted average TWA for an 8 hour work shift PPM Parts per Mmillion Prop 65 A California law requiring warnings for chemicals that are known to the state to be carcinogenic or to cause reproductive harm RCRA Resource Conservation and Recovery Act RTECS Registry of Toxic Effects of Chemical Substances SARA Superfund Amendments and Reauthorization Act a federal act administered by EPA SCBA Self Contained Breathing Apparatus STCC Standard Transportation Commodity Code STEL Short Term Exposure Level published by ACGIH Lowest published toxic concentration TLV Threshold limit value the ACGIH recommended TWA usually for an 8 hour work shift TWA Time weighted average UEL Upper explosive limit uorU Unknown UN United Nations This designation identifies hazardous chemicals in the process
32. d Biosystems provides the innovative products services and knowledge resources that make this new Integrated Science possible Worldwide Sales and Support Applied Biosystems vast distribution and service network composed of highly trained support and applications personnel reaches 150 countries on six continents For sales office locations and technical support please call our local office or refer to our Web site at www appliedbiosystems com Applera is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free In North America 1 800 345 5224 Fax 1 650 638 5884 04 2004 SS Applied AS BibEystems Part Number 4314377 Rev C
33. de protecao que deve ser ligado terra antes de se fazer qualquer outra ligacao el trica ao instrumento Um terminal marcado com este s mbolo recebe ou transmite tens o ou corrente alternada Um terminal marcado com este s mbolo recebe ou fornece tens o ou corrente alternada ou cont nua CUIDADO Este s mbolo indica a presenca de alta tensao e avisa o usu rio para proceder com cuidado CUIDADO Este s mbolo serve como alerta para que se consulte o manual a fim de se obter mais informa es e que se proceda com cuidado gt u HOO S mbolos A seguir apresentamos um gloss rio ilustrado de todos os s mbolos de alerta de n o el tricos seguran a n o relacionados eletricidade encontrados nos instrumento Applied Biosystems CUIDADO Este s mbolo representa um perigo devido a aquecedor no local Proceda com cuidado ao trabalhar em reas pr ximas a aquecedores para evitar queimaduras devidas ao contato com componentes quentes Este s mbolo indica que h um laser dentro do instrumento gt gt Instrument Safety 4 11 Simbolos de alerta de seguridad S mbolos el ctricos En la siguiente tabla se muestra un glosario ilustrado de todos los s mbolos el ctricos que se utilizan en los instrumentos de Applied Biosystems Este s mbolo indica la posici n de encendido del interruptor principal Este s mbolo indica la posici n de apagado del interruptor principal
34. e manual instructions and your local regulations GASEOUS CHEMICAL WASTE HAZARD Waste produced by Applied Biosystems instruments can be hazardous and can cause injury illness or death Operate a vented instrument only if it is connected in accordance with all requirements Venting hazardous waste may require local state provincial or national air permits Operate the system venting gaseous waste whenever the instrument power is on waste is in the waste container or reagents are on the instrument Handle all liquid solid and gaseous waste as potentially hazardous See the MSDSs provided by the manufacturer s of chemicals used at your site for more information The exhaust line must lead to the fume hood in a straight and upward direction Low points will allow condensation to collect preventing flow through the instrument Do not connect the waste vent line to a ductless hood or to a system that purifies and filters air and returns it to the room Always mix and prepare hazardous materials beneath an operating fume hood Dispose of waste in accordance with all local state provincial or national health and safety regulations and laws Instrument Safety 4 15 Acronyms and Abbreviations Acronyms and Abbreviations Used in MSDSs Introduction Organizations Regulations and Scientific Terminology MSDSs use acronyms and abbreviations for certain organizations government regulatio
35. eli e la mise la terre d un autre appareil Ce n est pas une borne de mise la terre prot g e Borne de mise la terre de protection devant tre reli e la terre avant d effectuer tout autre raccordement lectrique l appareil Borne recevant ou fournissant une tension ou un courant de type alternatif Borne pouvant recevoir ou fournir une tension ou un courant de types alternatif et continu ATTENTION Indique la pr sence d une haute tension et avertit l utilisateur de proc der avec pr caution ATTENTION Avertit l utilisateur de la n cessit de consulter le manuel pour obtenir davantage d informations et de proc der avec pr caution but DHO Symboles non Le tableau suivant donne la signification des symboles d alertes de securite non lectriques lectriques qui figurent sur les appareils Applied Biosystems ATTENTION Danger associ la pr sence d un appareil de chauffage Proc der avec pr caution pour viter de se br ler au contact de pi ces ou d l ments chauds Indique que l appareil renferme un laser gt gt 4 6 Instrument Safety Sicherheitswarnsymbole Elektrische Symbole Die folgende Tabelle enthalt Beschreibungen aller auf den Instrumenten von Applied Biosystems verwendeten elektrischen Symbole Wenn diese Symbole auf den Instrumenten erscheinen beachten Sie bitte die entsprechenden Sicherheitsvorkehrungen Dieses
36. ibility to Hazardous Waste 3 6 Chemical Safety 4 4 4 Characterize the waste generated with your applications Ensure the health and safety of all personnel in your laboratory Ensure that instrument waste is stored transferred transported and disposed of according to all local state provincial or national regulations Instrument Safety About This Chapter In This Chapter Safe Operation Routine Maintenance for Safe Operation This chapter provides you with the safety information you need to prepare your laboratory and personnel for the installation of the Procise or Procise cLC Protein Sequencer The safety labels and safety alert symbols that may be found on this instrument are provided in several languages Before operating this instrument read the information in the Procise User s Guide concerning hazards and potentialh hazards Ensure that anyone involved with the operation of the instrument is instructed in both general safety practices for laboratories and specific safety practices for the instrument Maintain the instrument in good working order If the instrument has been subjected to adverse environmental conditions such as fire flood or earthquake an Applied Biosystems service representative should inspect the instrument We recommend that an Applied Biosystems service representative check the instrument yearly to verify that The protective housing is functional Loose or distorted panels
37. iquement par un fusible de m me type et de m me courant nominal CAUTION HOT Attention Surface chaude DANGER High voltage Danger Haut Voltage WARNING To reduce the chance of electrical shock do not remove covers that require tool access No user serviceable parts are inside Refer servicing to Applied Biosystems qualified service personnel Avertissement Pour r duire la chance du choc lectrique ne retirez pas les couvertures qui exigent l acc s d outil Aucune pi ce utile d ulitisateur n est int rieur R f rez l entretien au personnel de service de Applied Biosystems DANGER Laser radiation when open and interlock defeated Avoid direct exposure to beam Danger Rayonnement de Laser si ouvert et couplage a d fait Evitez l exposition directe au faisceau CAUTION Moving parts Attention Pi ces mobiles Instrument Safety 4 3 Safety Alert Symbols Electrical Symbols The following chart is an illustrated glossary of all electrical symbols that are used on Applied Biosystems instruments Whenever such symbols appear on instruments please observe appropriate safety procedures This symbol indicates the On position of the main power switch This symbol indicates the Off position of the main power switch This symbol indicates the On Off position of a push push main power switch This symbol indicates that a terminal may be connected to another instrume
38. l velocity of airflow The minimum velocity at any point in the hood is 24 linear m min 80 linear ft min and the maximum velocity is 38 linear m min 125 linear ft min 4 A duct with a draw of 5 1 cm H O 2 0 in H O The waste exhaust system is composed of a fluid waste line and a gaseous waste exhaust line The fluid waste line is attached to a 4 L 1 gal polyethylene bottle in a secondary container provided by the user The gaseous waste exhaust line has a 9 5 mm outer diameter 3 8 in o d and conducts the waste fumes out the back of the instrument to the laboratory ventilation system for disposal CHEMICAL HAZARD Four liter reagent and waste bottles can crack and leak Each 4 L bottle should be secured in a low density polyethylene safety container with the cover fastened and the handles locked in the upright position Wear appropriate eyewear clothing and gloves when handling reagent and waste bottles For more information on handling liquid waste see Chapter 3 Follow these guidelines when connecting the tubing from the instrument s gaseous waste exhaust to your ventilation system Use the shortest possible length and the straightest possible run of polypropylene tubing Tubing length should not exceed 5 m 15 ft Make sure that the tubing does not have low points that can trap residue or condensation Fasten the tubing securely Use polypropylene or Teflon fasteners Do not use brass it corrodes Be ca
39. lvent A3 3 5 THF Tetrahydrofuran in Water 450 mL cLC Room temp 401886 Solvent B2 12 Isopropanol and Acetonitrile 450 mL cLC Room temp Computer The computer that accompanies this instrument comes with a Windows based Specifications operating system Do not use this computer for purposes other than operating this instrument Networking IMPORTANT Do not install optional or unnecessary software Applied Biosystems reserves the right to remove all software not supplied by Applied Biosystems in order to determine system functionality Applied Biosystems will supply and install but not support or connect a network card or install network connections Customers must make arrangements with their own network administration to have this done concurrent with or immediately after installation of the instrument Laboratory Safety Requirements On site Representative Required Safety Equipment We request that a representative from your laboratory be in the vicinity and available to the Applied Biosystems engineer at all times while the engineer is at your facility This person should be familiar with laboratory safety procedures and know the location of all the safety equiptment Your laboratory has specific safety practices and policies designed to protect laboratory personnel from potential hazards that are present We expect that all applicable safety related procedures will be followed at all times The following safety equipme
40. mables Required 2 4 Site Preparation its availability Date Y Confirmed ltem Equipment Safety glasses lab coats Waste bottle with secondary containment Windows PC compatible printer this cannot be ordered with the instrument and is optional A two stage pressure regulator with dual gauges output range 0 80 psi high pressure range 0 3000 psi and a Compressed Gas Association CGA 580 cylinder adapter for each gas cylinder Approved strap or clamp to safely secure gas cylinders to the wall or bench table Consumable Supplies Acetone HPLC grade Additional computer supplies paper disks etc Additional Applied Biosystems reagent kits Argon gas cylinders 2 of 99 998 purity or greater Chemically resistant powder free disposable gloves Deionized water is on site Methanol in wash bottle Items Shipped with This Instrument Summary List Do Not Move or Unpack Instrument Unpacking Chemicals The Procise and Procise cLC Protein Sequencer is shipped with the following Procise or Procise cLC in 1 box with Procise User s manual set P N 4340645 Accessories Detector in 1 box HPLC pump in 1 box Installation chemical kit Procise P N 401690 and Procise cLC P N 402064 Computer system with Microsoft Windows based operating system and monitor in 2 boxes 9 IMPORTANT You must unpack the installation chemi
41. n 2 then 1 From any other See the specific region under To Contact Technical Support by country Telephone or Fax Outside North America To view download or order documents through the Applied Biosystems web site Step Action 1 Go to http www appliedbiosystems com 2 Click SERVICES amp SUPPORT at the top of the page click Documents on Demand then click MSDS Ordering MSDSs from Other IManufacturers MSDSs for Chemicals Not Supplied by Applied Biosystems To view download or order documents through the Applied Biosystems web site Step Action 3 Click MSDS Index search through the list for the chemical of interest to you then click on the MSDS document number for that chemical to open a pdf of the MSDS For chemicals not manufactured or distributed by Applied Biosystems call the chemical manufacturer Applied Biosystems does not furnish MSDSs for chemicals used on this instrument that are not manufactured or distributed by Applied Biosystems Contact the manufacturer s of those chemicals to obtain additional MSDSs Brief warnings for these chemicals are listed below CHEMICAL HAZARD Acetone is a flammable liquid and vapor Exposure may cause eye skin and upper respiratory tract irritation Prolonged or repeated contact may dry the skin Exposure may cause central nervous system depression Keep away from heat sparks and flame Read the MSDS and f
42. ns common scientific terminology units of measurement and chemicals This appendix is provided to help you understand these references while reading the MSDSs for the chemicals used in your laboratory The following table lists acronyms and abbreviations for organizations government regulations and scientific terminology Term Explanation ACGIH American Conference of Governmental Industrial Hygienists CAS Chemical Abstract Service Reference Number for Specific Pure Chemical cc Closed cup testing of flash point CFR Code of Federal Regulations Regulations published by the United States Government CERCLA Comprehensive Environmental Response Compensation and Liability Act Superfund is a federal law administered by EPA DFG MAK Federal Republic of Germany s Maximum Contamination Value in the workplace similar to PEL in the USA DOT United States Department of Transportation regulates transportation of hazardous material USA EPA United States Environmental Protection Agency regulates use disposal or emission of hazardous material USA IDLH Immediate Danger to Life and Health LCio Lowest published lethal concentration LCso Lethal concentration in air that kills 50 of a specified population LDso Lethal dose that kills 50 of a specified population LEL Lower explosion limit MSHA Mine Safety and Health Administration recommends respirators NFPA National Fire Prote
43. nt must be available Fire extinguisher ltis your responsibility to provide appropriate fire extinguishers for use on or near Applied Biosystems equipment The types and sizes of fire extinguishers shall be suitable for use on electrical and chemical fires as specified in currently prevailing codes regulations and or standards and with the approval of the Fire Marshall or other authority having jurisdiction The installation of appropriate fire extinguishers shall be in addition to other fire protection systems and not as a substitute or alternative to them Eyewash Safety shower Eye and hand protection Adequate ventilation First aid equipment Spill cleanup equipment Protection from any sources of hazardous chemicals radiation lasers radioisotopes contaminated equipment radioactive wastes etc and potentially infectious biological material that may be present in the area where Applied Biosystems engineer s will be working Site Preparation 2 7 Laboratory Space Required Dimensions and The Procise or Procise cLC Protein Sequencer and computer have the following Weight dimensions Computer Complete Dimensions Sequencer PTH system system system Width mm 735 525 690 1950 Inches 29 20 27 76 Depth mm 505 530 710 710 Inches 20 21 28 28 Height mm 635 480 455 635 Inches 25 19 18 25 Weight kg 55 45 41 141 Ibs 121 99 76 6 297 Inst
44. nt s signal ground reference This is not a protected ground terminal This symbol indicates that this is a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument A terminal marked with this symbol either receives or delivers alternating current or voltage ut 00 A terminal marked with this symbol can receive or supply an alternating and a direct current or voltage CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution CAUTION This symbol alerts you to consult the manual for further information and to proceed with caution Nonelectrical The following is an illustrated glossary of all nonelectrical safety alert symbols found Symbols on Applied Biosystems instruments 44 Instrument Safety CAUTION This symbol illustrates a heater hazard Proceed with caution when working around these areas to avoid being burned by hot components N A This symbol indicates that a laser is present inside the instrument ESI abe MYU Applied Biosystems ZA EP o FLA gt BE BA
45. nuale per ulteriori informazioni e procedere con cautela Segue un glossario illustrato dei simboli degli allarmi di sicurezza non elettrici trovati su strumenti Applied Biosystems ATTENZIONE questo simbolo illustra un rischio da alte temperature Procedere con cautela quando si lavora in queste aree per evitare ustioni causate di componenti a temperature elevate gt gt Questo simbolo indica la presenza di laser nello strumento 7 Applied Biosystems
46. of the nominal value above a power line regulator may be required High or low voltages can have adverse effects on the electronic components of the instrument Short duration high voltage spikes often cause random failures in microprocessor controlled instrumentation These spikes can be caused by other devices using the same power source refrigerators air conditioners and centrifuges or by outside influences such as lightning A dedicated line and ground between the instrument and building main electrical service will prevent such problems If your environment contains devices that are electrically noisy or you are in an area with frequent electrical storms a line conditioner with a recommended capacity of 2 0 kVA will enhance the reliability of your system The instrument has been designed to pause from short periods of power outage loss To continue operation you must resume the run from the software provided that the line voltage did not become excessively noisy before the outage If you want increased protection during a power outage install an uninterruptible power supply UPS with a capacity of 2 0 kVA In the United States the Clary Corporation a UPS vendor can be reached at 1 800 326 6480 ELECTRICAL SHOCK HAZARD Severe electrical shock which could cause physical injury or death can result from working on an instrument when the high voltage power supply is operating To avoid electrical shock disconnect the power sup
47. of worldwide transportation Units of Measure The following table lists abbreviations for common units of measure Abbreviation Unit of Measure number C degrees Celsius F degrees Fahrenheit HL microliter um micron mol micromole AUFS absorbency units full scale Btu British thermal unit ft foot gal gallon h hour i d inside diameter in inch kVA kilovoltampere L liter m meter mg milligram A 2 Acronyms and Abbreviations Abbreviation Unit of Measure mL milliliter mm millimeter o d outside diameter P N part number psi pounds per square inch sec second V volt VA voltampere VaC volts alternating current W watt Chemicals The following table lists abbreviations for common chemicals Abbreviatiom Definition A adenine AA amino acid 1Ac acetyl Acl acetylimidazole Acm acetamidomethyl Ac O acetic anhydride ACN acetonitrile ACT activator vessel BHA resin benzhydrylamine resin tBoc tert butyloxycarbonyl Bzl benzyl Br Z 2 bromobenzyloxcarbonyl t Bu tert butyl C cytosine CHO formyl CH Bz 4 methylbenzyl CH 0Bzl 4 methoxybenzyl CI Z 2 chlorobenzyloxycarbonyl CPG Controlled Pore Glass DCA dichloroacetic acid DCC dicyclohexylcarbodiimide DCM dichloromethane DCU dicyclohexylurea DIEA diisopro
48. ollow the handling instructions Wear appropriate protective eyewear clothing and gloves CHEMICAL HAZARD Methanol is a flammable liquid and vapor Exposure causes eye and skin irritation and may cause central nervous system depression and nerve damage Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves CHEMICAL HAZARD Sodium Dodecyl Sulfate SDS Exposure causes eye skin and respiratory tract irritation Exposure may cause severe allergic respiratory and skin reaction Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves Chemical Safety 3 3 Hazardous Chemicals Overview Handling Hazardous Chemicals 3 4 Chemical Safety This instrument may use chemicals that are hazardous PIZZA CHEMICAL HAZARD Hazardous chemicals used with this instrument can cause injury illness or death Handle all chemicals as potentially hazardous Chemicals are classified as hazardous when they are physically hazardous or if they can cause acute or chronic health hazards upon exposure Physically hazardous chemicals are materials that are flammable combustible under compression gases explosive oxidative organically peroxidic pyrophoric reactive or unstable or water reactive Chemicals that may cause health hazards include carcinogens materials that are toxic or highly toxic reproductive toxins irritants corrosi
49. ply to the instrument unplug the power cord and wait at least 1 minute before working on the instrument Pressurized Gas and Regulator Requirements Gas Cylinder Pressure Regulator Attaching the Cylinder You must supply the required gas cylinder and accessories for installation This instrument requires one size 1 A argon gas cylinder that holds approximately 7 2 m3 257 ft 3 of gas when full Use only prepurified argon of 99 998 or greater purity An additional argon cylinder should be ordered in advance for the ongoing operation of the instrument Damage to the instrument and its products can result from using impure argon gases other than argon or an inadequate amount of argon NUNNE EXPLOSION HAZARD Pressurized gas cylinders are potentially explosive Always cap the gas cylinder when it is not in use and attach it firmly to the wall or gas cylinder cart with approved brackets chains or clamps A two stage pressure regulator with dual gauges output range 0 80 psi high pressure range 0 3000 psi and a Compressed Gas Association CGA 580 cylinder adapter with a needle type shutoff valve on the exit side are required The needle valves should have Swagelok type end fittings ready for connection to 25 in 6 35 mm o d tubing The second stage output of the regulator should be set at approximately 65 psi minimum 448 1 kPa BOTTLE EXPLOSION HAZARD Bottle explosion can cause severe physical injury To prevent
50. pylethylamine DMAP 4 dimethylaminopyridine DMF dimethylformamide DMSO dimethylsulfoxide DNA deoxyribonucleic acid Acronyms and Abbreviations A 3 Abbreviatiom Definition Dnp 2 4 dinitrophenyl Et ethyl EtOH ethanol Fmoc 9 fluorenylmethyloxycarbonyl G guanine HBTU 2 1H benzotriazol 1 yl 1 1 3 3 tetramethyl uronium hexafluorophosphate HLP high loaded polystyrene HMP resin p hydroxymethylphenoxymethyl polystyrene resin HOAc acetic acid mBHA resin 4 methylbenzhydrylamine resin MeOH methanol Mob 4 methoxybenzyl Mtr 4 methoxy 2 3 6 trimethyl benzene sulfonyl Mts mesitylene 2 sulfonyl NMI 1 methylimidazole NMP N methylpyrrolidone N methyl 2 pyrrolidone OBt ethyl ester OMe methyl ester PAM resin phenylacetamidomethyl resin PEG polyethylene glycol RV reaction vessel SSPS solid phase peptide synthesis T thymine TETD tetraethylthiuram disulfide TFA trifluoroacetic acid TFMSA trifluoromethane sulfonic acid THF tetrahydrofuran Tos 4 toluenesulfonyl tosyl Tri trityl U uracil Z benzyloxcarbonyl A 4 Acronyms and Abbreviations Science iScience To better understand the complex interaction of biological systems life scientists are developing revolutionary approaches to discovery that unite technology informatics and traditional laboratory research In partnership with our customers Applie
51. r 1 800 831 6844 1 650 638 5981 then press 8 DNA Synthesis 1 800 831 6844 1 650 638 5981 then press 21 Fluorescent DNA Sequencing 1 800 831 6844 1 650 638 5981 then press 22 Fluorescent Fragment Analysis includes GeneScan applications 1 800 831 6844 1 650 638 5981 then press 23 Product or Product Area Telephone Dial Fax Dial Integrated Thermal Cyclers ABI PRISM 877 and Catalyst 800 instruments 1 800 831 6844 then press 24 1 650 638 5981 ABI PRISM 3100 Genetic Analyzer 1 800 831 6844 then press 26 1 650 638 5981 Biolnformatics includes BioLIMS BioMerge and SQL GT applications 1 800 831 6844 then press 25 1 505 982 7690 Peptide Synthesis 433 and 43X Systems 1 800 831 6844 then press 31 1 650 638 5981 Protein Sequencing Procise Protein Sequencing Systems 1 800 831 6844 then press 32 1 650 638 5981 PCR and Sequence Detection 1 800 762 4001 then press 1 for PCR 2 for the 7700 or 5700 6 for the 6700 or dial 1 800 831 6844 then press 5 1 240 453 4613 Voyager MALDI TOF Biospectrometry and Mariner ESI TOF Mass Spectrometry Workstations 1 800 899 5858 then press 13 1 508 383 7855 Biochromatography BioCAD Workstations and Poros Perfusion Chromatography Products 1 800 899 5858 then press 14 1 508 383 7855 Expedite Nucleic acid Synthesis
52. r manufacturer When hazards exist warnings are prominently displayed on the labels of all chemicals MSDSs are supplied by the chemical manufacturer and provide information about Physical characteristics Safety precautions Health hazards First aid Spill cleanup Disposal procedures VNDE CHEMICAL HAZARD Be sure to familiarize yourself with the MSDSs before using reagents or solvents Explanations of acronyms and abbreviations used in MSDSs can be found in Appendix A of this guide Chemical manufacturers supply a current MSDS before or with shipments of hazardous chemicals to new customers and with the first shipment of a hazardous chemical after an MSDS update Replace the MSDSs in your files regularly so that the safety information is current You can order free copies of MSDSs for chemicals manufactured or distributed by Applied Biosystems using the contact information below To order documents by automated telephone service 1 From the U S or Canada dial 1 800 487 6809 or from outside the U S and Canada dial 1 858 712 0317 2 Follow the voice instructions to order documents for delivery by fax Note There is a limit of five documents per fax request To order documents by telephone In the U S Dial 1 800 345 5224 and press 1 To order in English dial 1 800 668 6913 and press 1 then 2 then 1 In Canada To order in French dial 1 800 668 6913 and press 2 the
53. r disposing of hazardous waste Read all applicable MSDSs before handling or disposing of hazardous waste Ensure that the waste container is correctly installed Always handle hazardous materials beneath a fume hood that is connected in accordance with all installation requirements Always wear chemical resistant gloves safety glasses and protective clothing when handling hazardous waste material During transfer ensure that the waste container is tightly sealed with the waste cap provided Dispose of hazardous waste in accordance with all local state provincial or national regulations Chemical Safety 3 5 Storing Hazardous CHEMICAL STORAGE HAZARD Never collect or store waste in a glass Waste Container because of the risk of breaking or shattering Reagent and waste bottles can crack and leak Each waste bottle should be secured in a low density polyethylene safety container with the cover fastened and the handles locked in the upright position Wear appropriate eyewear clothing and gloves when handling reagent and waste bottles The following are guidelines for storing hazardous waste Always use secondary containment when storing chemical waste Store waste for only short periods of time Store only small amounts of waste in the laboratory Store waste away from direct sunlight or sources of heat on or off the instrument Disposing of As the generator of potentially hazardous waste it is your respons
54. reful not to puncture tubing Place the tubing away from sources of potential damage such as heat flame or points of contact rubbing with other objects Place the tubing end as far as possible into the duct canopy or hood Oncoming air movement through the duct or canopy should not face the open end of the tubing Connect the fluid waste line from the instrument to the waste bottle so that it drops vertically Doing so prevents liquid and waste from accumulating and blocking the flow Follow these guidelines in connecting the flexible duct from the instrument s system vent to your laboratory ventilation system Use the shortest possible length and straightest possible run of flexible duct Make sure the flexible duct does not have low points that can trap residue or condensation Heat Production Laboratory Ventilation System Canopy and or Duct The thermal output of the instrument system is 5690 Btu h 1666 W Consult your facilities department regarding ventilation requirements for this level of heat output Building air intake Must not draw gaseous waste back into the building Duct Movement of gaseous waste System vent Canopy Air flow 3 4 indicator Waste bottle Instrument exhaust line Ventilation Operation GR1743a Duct work Verify that ducts are constructed of materials that
55. rld Location Voltage VAC Frequency Japan 220 10 50 60 Hz 1 USA Canada 120 10 50 60 Hz 1 Europe pre 1992 220 10 50 60 Hz 1 EC 230 10 50 60 Hz 1 UK pre 1992 240 6 10 50 60 Hz 1 Australia 240 6 10 50 60 Hz 1 The electrical receptacle must have a dedicated 2 0 kVA power line and ground or a 2 0 kVA power line with a line conditioner or uninterruptible power supply UPS This instrument requires a Nema 5 15 receptacle in the U S The electrical receptacle must be located within 2 5 m 8 ft of the instrument rear panel Do not use extension cords This instrument is rated for a maximum output of 400 VA The instrument system is supplied with power cords that plug into a powerstrip The powerstrips have various plug ends that are configured for different electrical receptacles The computer can be plugged into any standard electrical receptacle after it has been configured for the proper voltage Certain types of electrical noise are greatly exaggerated by poor or improper electrical ground connections To prevent these problems it is very important to have a dedicated line and ground between the instrument and building main electrical service Site Preparation 2 15 Power Line Regulator Voltage Spikes Power Outages Electric Shock Warning 2 16 Site Preparation In areas where the supplied power is subject to voltage fluctuations exceeding 10
56. rm that everything is checked off before making an appointment for installation Components The following components are supplied by Applied Biosystems Check off the items Supplied by Applied after performing the actions Biosystems Date Y Confirmed Action Received instrument s and inspected the crates and boxes Counted the number of boxes and checked if Items Shipped with This Instrument on page 2 5 are the same as ordered Reported any discrepancies in instrument system configuration or damage to the crates or boxes to your Applied Biosystems service representative Left the boxes intact for the Service Engineer to unpack Read all sections of this Site Preparation and Safety Guide Read the MSDSs provided with the installation chemistry kit Unpacked and stored contents of installation chemistry kit On Site Personnel Check off the items below after confirming the following with your laboratory personnel Date Y Confirmed Action Have available 2 uninterrupted days for in lab training during installation Designated one person to be the laboratory safety representative This person is familiar with laboratory safety procedures knows the location of all safety equipment and must be available to the Applied Biosystems engineer while the engineer is at your facility Designated 1 person to be present to assist the service engineer in lifting and or positioning the inst
57. rument Laboratory Facility Check off each item as you verify that the instrument location meets each of the Requirements requirements Date Y Confirmed Requirement Safety Met requirements specified in Laboratory Safety Requirements on page 2 7 2 2 Site Preparation Y Date Confirmed Requirement Instrument location Verified a pathway from the receiving dock to the storage area if applicable to accommodate the instrument s 102 cm 40 in crated width Verified a pathway from the storage area to the laboratory to accommodate the instrument s 74 cm 29 in width Laboratory space is of correct dimensions to accommodate the system and its ventilation clearances and is situated so that the instrument is accessible to the installer on all four sides See Laboratory Space Required on page 2 8 Total vertical clearance of 38 cm 15 in is required to raise the cover of the instrument The computer will be no more than 2 m 6 ft from the instrument Laboratory bench is of the correct dimensions and weight tolerance to accommodate the system and computer See Laboratory Space Required on page 2 8 Situated the computer to allow for proper ergonomics during use Ventilatio n and Waste A properly functioning ventilation system is within 5 m 16 ft of the instrument Provided a system for exhausting gaseous instrument
58. rument Location Plan on providing sufficient laboratory space for the instrument system as well as the following accommodations 2 8 Site Preparation 30 cm 1 ft of clearance at the rear of the instrument for adequate ventilation 38 cm 15 in of vertical clearance to raise the instrument cover Access to all four sides of instrument for servicing Do not block access to the rear of the instrument Laboratory bench of correct dimensions and weight tolerance to accommodate the system Sufficient space within approximately 1 5 m 5 ft of the instrument to safely secure at least one size 1A Argon gas cylinder Sufficient space to the right side of the instrument for solvent and waste bottles The computer must be no more than 2 m 6 ft from the instrument The computer monitor and keyboard situated to allow for proper ergonomics during use The following guidelines are recommended Benchspace or pull out tray provided so that the keyboard is positioned directly in line with the monitor Open space under the keyboard so that the operator can sit comfortably directly facing the monitor Workspace provided next to the monitor Assisting the Engineer Move the Instrument Typical Laboratory Layout Power cord At least one person will be required to assist the service engineer place the instrument onto the laboratory bench Do not unpack or move the instrument before the service engineer arrives to
59. ste may require local state provincial or national air permits Site Preparation 2 13 Laboratory Environmental Requirements Altitude Temperature and Humidity Pollution This instrument is for indoor use only and for altitudes not exceeding 2000 m 6500 ft above sea level The laboratory temperature should be maintained at 15 30 C 59 85 F The instrument can tolerate up to 80 non condensing relative humidity Avoid placing the instrument adjacent to heaters cooling ducts or in direct sunlight The installation category overvoltage category for this instrument is II and it is classified as portable equipment The instrument has a pollution degree rating of 2 and may be installed in an environment that has nonconductive pollutants only Safety and Electromagnetic Compatibility EMC Standards U S and Canadian Safety Standards c UL us Canadian EMC Standard European Safety and EMC Standards CE 2 14 Site Preparation This section provides information on U S and Canadian Safety Standards Canadian EMC Standard European Safety and EMC Standards AustralianEMC Standards This instrument has been tested to and complies with standard UL 3101 1 Safety Requirements for Electrical Equipment for Laboratory Use Part 1 General Requirements This instrument has been tested to and complies with standard CSA 1010 1 Safety Requirements for Electrical Equipment for Measurement Control and
60. tallation u a EA HER DU 0 1 1 About Sei Cd I MIN a Cx EU ERU WR 1 2 About Chemical Safety or ee RR ep A 1 2 Technical Support 111 RE Re EE 1 4 Site Preparation Before You St it 1111 1 11 1 11 1 2 1 Pr mstallation Checklists s reisis saio 32 32 DADE RR Erde IRE Pete DR CR Ee Reds 2 2 Items Shipped with This Instrument 2 5 Laboratory Safety Requirements 2 7 Laboratory Space Required 2 8 Laboratory Ventilation Requirements 2 10 Laboratory Environmental Requirements 2 14 Safety and Electromagnetic Compatibility EMC Standards 2 14 Electrical Requirements cete e 2 15 Pressurized Gas and Regulator Requirements 2 17 Chemical Safety NM 33 7 800 3 1 Material Safety Data Sheets Overview 3 2 Hazardous Chemicals cin ERREUR Aa S ed 3 4 Hazardous Waste ass 1111 4100 3 5 Instrument Safety About This Chapter o lr Sees SE 4 1 Instrument Labeling 23200 11 4 2 Ssatety Alert Symbols a foes Gok E PORE ERR ER IUS anaes 4 4 Safety Alert Symbols Chinese es 4
61. tate provincial or national laws and regulations related to chemical storage handling and disposal Chemical Waste PL CHEMICAL WASTE HAZARD Wastes produced by Applied Biosystems Hazard Warning instruments are potentially hazardous and can cause injury illness or death Read and understand the material safety data sheets MSDSs provided by the manufacturers of the chemicals in the waste container before you store handle or dispose of chemical waste Handle chemical wastes in a fume hood Minimize contact with and inhalation of chemical waste Wear appropriate personal protective equipment when handling chemicals e g safety glasses gloves or protective clothing After emptying the waste container seal it with the cap provided Dispose of the contents of the waste tray and waste bottle in accordance with good laboratory practices and local state provincial or national environmental and health regulations Introduction 1 3 Technical Support Contacting You can contact Applied Biosystems for technical support by telephone or fax by Technical Support e mail or through the Internet You can order Applied Biosystems user documents MSDSs certificates of analysis and other related documents 24 hours a day In addition you can download documents in PDF format from the Applied Biosystems Web site please see the section To Obtain Documents on Demand following the telephone information below To Contact Techni
62. user documentation Each word implies a particular level of observation or action as described below Note Calls attention to useful information IMPORTANT Indicates information that is necessary for proper instrument operation FAN Cautions the user that a potentially hazardous situation could occur causing injury to the user or damage to the instrument if this information is ignored NVN Warns the user that serious physical injury or death to the user or other persons could result if these precautions are not taken PME Indicates an imminently hazardous situation that if not avoided will result in death or serious injury About Chemical Safety About Material Safety Data Sheets MSDSs About Waste Disposal 1 2 Introduction Some of the chemicals used with this instrument may be listed as hazardous by their manufacturer When hazards exist warnings are prominently displayed on the labels of all chemicals Chemical manufacturers supply a current material safety data sheet MSDS before or with shipments of hazardous chemicals to new customers and with the first shipment of a hazardous chemical after an MSDS update MSDSs provide you with the safety information you need to store handle transport and dispose of the chemicals safely We strongly recommend that you replace the MSDSs in your files each time you receive one packaged with hazardous chemicals PAULINE CHEMICAL HAZARD Be sure to familiarize yourself with the M
63. ves sensitizers materials that are toxic to the liver kidney or blood forming hematopoietic system and agents that damage the lungs skin eyes or mucous membranes Here are some of the important requirements for handling hazardous chemicals Read and understand all applicable MSDSs before handling hazardous chemicals When replacing reagents always install a new bottle on the instrument Do not add new solution to previously used reagent bottles Some chemicals reduce the integrity of glass bottles As a result repeated use beyond 6 weeks may result in the bottle fracturing when it is pressurized during operation Always wear gloves safety glasses and protective clothing when handling chemicals Always provide adequate ventilation when handling chemicals Some chemicals require handling only in a properly functioning fume hood Provide secondary containment for all reagent bottles Do not store chemicals in direct sunlight or heat on or off the instrument Hazardous Waste Overview This instrument may generate hazardous waste Instrument Waste System Handling Chemical Waste CHEMICAL WASTE HAZARD Wastes produced by Applied Biosystems instruments are potentially hazardous and can cause injury illness or death Read and understand the Material Safety Data Sheets MSDSs provided by the manufacturers of the chemicals in the waste container before you store handle or dispose
64. vides orientation to this guide and Customer Support contact information Site Preparation Provides installation requirements and the preinstallation checklists Chemical Safety Provides general chemical handling guidelines and instrument waste profiles Instrument Safety Explains safety alert symbols on instrument and shows instrument input and output connections Acronyms and Abbreviations Explains terms used in Material Safety Data Sheets MSDSs and in this guide About Preinstallation Preparing Your Site Choosing a Location Preinstallation checklists start on page 2 2 of this guide An Applied Biosystems employee will contact you to verify that everything on the checklists has been done before making an appointment for installation When deciding where to put the instrument keep in mind the following You must be able to disconnect the main power supply to the instrument immediately if necessary You must be able to comply with local state provincial or national air quality regulations while venting the exhaust from this instrument Introduction 1 1 About Safety Using the Instrument Correctly Documentation User Attention Words Use this instrument as specified by Applied Biosystems If the instrument is used in a manner not specified by the manufacturer the protection provided by the instrument may be impaired Five user attention words appear in the text of all Applied Biosystems
65. will not protect the user or the equipment Airflow is not hindered in any way Instrument Safety 4 1 Instrument Labeling Safety Labels Safety labels are located on the instrument Each label consists of a Signal Word panel and a Message panel A Safety Alert symbol indicates a potential personal safety hazard If multiple hazards exist the signal word corresponding to the greatest hazard is used Signal Words XIAN Cautions the user that a potentially hazardous situation could occur causing injury to the user or damage to the instrument if this information is ignored PNA Warns the user that serious physical injury or death to the user or other persons could result if these precautions are not taken PME Indicates an imminently hazardous situation that if not avoided will result in death or serious injury 4 2 Instrument Safety Labels That May Be The following Danger Caution and Warning labels listed in English and French Found On The below may be found on your instrument Instrument English French CAUTION Hazardous chemicals Read the Material Safety Data Sheets before handling Attention Produits chimiques dangeureux Lire les fiches techniques de s ret de mat riels avant la manipulation des produits CAUTION Hazardous waste Read the Waste Profile before handling or disposal Attention D chets dangeureux Lire les renseignements sur les d chets avant de manipuler ou d eliminer
66. zeigt das Vorliegen eines Lasers im Innern des Instruments an Instrument Safety 4 7 Simboli degli allarmi di sicurezza Simboli elettrici Simboli non elettrici 4 8 Instrument Safety La tabella seguente un glossario illustrato di tutti i simboli elettrici utilizzati su strumenti Applied Biosystems Ogni volta che tali simboli compaiono sugli strumenti rispettare le procedure di sicurezza appropriate Questo simbolo indica la posizione ON dell interruttore di alimentazione generale Questo simbolo indica la posizione OFF dell interruttore di alimentazione generale Questo simbolo indica la posizione ON OFF di un interruttore di alimentazione generale a pulsante Questo simbolo indica che un terminale pu essere collegato al riferimento di terra del segnale di un altro strumento Non e un terminale di terra protetto Questo simbolo indica un terminale protettivo di messa a terra che deve essere collegato a terra prima di realizzare qualsiasi altro collegamento elettrico allo strumento Un terminale contrassegnato con questo simbolo riceve o fornisce tensione o corrente alternata Un terminale contrassegnato con questo simbolo pu ricevere o fornire tensione o corrente alternata e continua ATTENZIONE questo simbolo indica la presenza di alta tensione e invita l utente a procedere con cautela gt gt Dr ATTENZIONE questo simbolo invita l utente a consultare il ma

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