User`s manual - Fisher UK Extranet
Contents
1. Liquid is not nozzle L provided cleaning wire sucked in X ring is worn or V Liquid leakage damaged Replace the X ring set with the new set Plunger is damaged or rusty KL p Replace the X ring set and Plunger set with the new sets J from tip Nozzle tip attachment Replace the nozzle set with the L part is damaged new set Attached tips are loose Attach the tips firmly NS N Push button movement is poor Plunger has shifted Press the push button a few times X ring is damaged KL Replace the X ring set with the new set gt No grease on the plungers and or X rings Coat the plungers and or X rings lightly with the provided grease P Liquid in the nozzle KL If immediately after suction or if there just some adherence of liquid disassemble and clean the parts Looseness of body and casing amp Ring is loose Tighten the ring Connection screw is 4 loose Tighten the connection screw with the provided wrench If the problem persists even after the above points have been checked stop usage of the unit immediately and consult with the sales outlet where the unit was purchased Caution At this time please confirm that there has been no contamination by chemicals that are harmful to the human body 12. O 4 Return the push button gently to its original position The liquid will be pulled into the tip At this time pause for 1 second to wait for complete suction of the liquid Fig D 5 Withdraw the tip gently from the liquid By doing this gently hardly any of the liquid will remain on the outside surface of the tip If liquid does remain remove it by paper or something similar being careful not to touch the tip s point 4 Note Do not carry out suction from the c position shown in Fig C liquid will be pulled into the nozzle if done from this a position Note Operate the push button gently b Releasing quickly will cause liquid to be pulled into the inside of the unit causing possible loss of accuracy Fig C sin ih Fig D 3 Discharge 1 Touch the top of the tip to the inside of the receiving vessel Fig D 2 Gently depress the push button from a to b Wait abut 1 second then depress to the c position The liquid is now discharged Fig D 3 Press the eject button to remove the used tip Fig D Do not touch the tip during use or after use when liquid that is harmful to the body is being used Disassembly and assembly of the airtight unit If any of the problems included in the Troubleshooting section of this
3. manual arise use the following procedure to disassemble and inspect this unit 1 Disassembly 1 Turn the ring in the direction of the arrow and remove the casing from the handle Fig 1 Note If the connection screw is loosened tighten it with the provided wrench Fig 2 2 Remove the screw that holds the ejector and remove the ejector from the casing Fig 3 3 Remove the screw that holds the casing Fig 4 4 Lightly press the nozzle and remove the casing A set as shown in Fig 4 5 Remove each part Fig 5 2 Assembly 1 Attach each part to casing B Fig 5 2 Assemble and attach the casing A set Fig 4 3 Assemble and attach the ejector to the assembled casing Fig 3 4 Assemble and attach the casing set to the handle Fig 1 Note When assembling be sure to set the counter to the maximum volume range Parts will not mesh properly Note If the connection screw is loosened tighten it with the provided wrench Fig 2 Handle q oii QD Fig 1 Connection B lt screw Fig 2 Handle H Casing Ejector set Ejector plate holding screw Fig 3 Casing Aset Ejector button Casing holding screw Fig 4 Plunger head spring Plunger holder set Plunger set X ring holder Nozzle Bush Nozzle spring First spring Nozzle spring holder Position to gear plungears Fi
4. may result in loss of accuracy Do not touch the unit directly with your hand just after autoclaving and drying A because the unit is extremely hot during autoclaving and drying Directly touching the hot unit may cause an accident Code Variable range Measured volume Accuracy Precision Model No u 2 ul 00 NP7 Tul 8 0 lt 4 0 8V 12V 0 5 10 5u 4 0 lt 2 0 10 u 2 0 lt 1 0 00 NP7 5u 3 0 lt 1 5 8S 12S 5 50 25 u l 2 0 lt 0 8 50 p 4 1 0 lt 0 5 00 NP7 40 u l 1 4 lt 0 5 8L 12L 40 200 100 u 2 1 0 lt 0 4 200 u 0 9 lt 0 3 00 NP7 50 u 2 1 4 lt 0 7 8K 12K 50 300 150 u 4 1 0 lt 0 5 300 u 0 6 lt 0 2 9 e Tips autoclavable Code ur Color Applicable model Tip length Q ty bag u BMT UT 0 1 10 Natural 00 NP7 8 12V 31 0 mm 1000 BMT SE 5 200 Natural 00 NP7 8 12S 8 12L 51 1 mm 1000 BMT K 50 300 Natural 00 NP7 8 12K 58 9 mm 1000 Rack tips autoclavable Code ae P Color Applicable model Q ty box BMT UTR 0 1 10 Natural 00 NP7 8 12V 960 96 x 10 cases BMT SER 5 200 Natural 00 NP7 8 12S 8 12L 960 96 x 10 cases BMT KR 50 gt 300 Natural 00 NP7 8 12K 960 96 x 10 cases R 1 ECALIBRATION PROCEDURE Remove the cap by pulling it out with your fingers Fig 6 2 Loosen the lock handle by turni
5. the instrument may be damaged or deteriorate Do not operate when the liquid amount is less than the set volume It may cause the liquid to scatter into the main body and the instrument may deteriorate in the quality Operation 1 Volume setting 1 Turn the lock handle in the unlock direction and loosen it Fig A 2 Turn the push button to set the digital counter to the desired volume When increasing volume first set the scale a half revolution further and then set itto the desired amount When decreasing the volume the scale can be set directly to the desired amount At this time set the counter s scale to the pointer mark red located at the bottom section of the counter window Fig B 3 After volume setting turn the lock handle in the lock direction and tighten Fig A Note Do not exceed the specified liquid volume limit otherwise the instrument may be damaged or deteriorate in the quality Note After changing the volume use the unit after first carrying out a trial operation Digital counter 401 200 u 0 2 4 0 0 9 X Pointer mark Red Fig A Fig B 2 Suction 1 Attach the disposable tip to the nozzle attach from the rack when doing so 2 Press the push button down to a gt b Fig C 3 With the push button pressed down insert the top of the tip to a point suitable for suction of the desired volume Fig D
6. tip eject function The tips can be removed without touching them by fingers The simple casing structure enables easy maintenance Standard accessories Tip 80h x 16 12 CN x 24 Silicone grease x 1 Cleaning wire x 1 Wrench x1 Please make sure that none of the above accessories are missing before using this unit m Safety precautions For safe and proper use of the Nichipet 7000 please be sure to carefully read the Safety precautions in this section and CAUTIONS on the next page before starting work with it e The contents of CAUTIONS are matters that require the user s attention not only for using the Nichipet 7000 properly but for preventing the user from accidents and physical damage After reading this manual please keep it in a convenient place for quick reference Please observe the following cautions to ensure the safe use of this product A CAUTIONS Be sure to observe the following instructions for using your Nichipet 7000 properly and safely If the user uses the Nichipet 7000 in the wrong way disregarding the following instructions it may result in injury to the user or and other persons or physical damage to this instrument or and other equipment AUN NO 0 10 11 12 13 Do not use for any other purpose than handling of liquid Never modify this instrument as this m
7. to direct sunlight 2 hours before operation as well as during operation Exposure to sunlight may result in loss of accuracy Avoid touching the tip and nozzle sections as much as possible just before operating Warming of these parts may result in loss of accuracy Please use the forward method when sampling the operation method used in this manual Using a different method may result in loss of accuracy Press the push button gently If pressed quickly liquid will be pulled into the inside of the unit which may result in loss of accuracy Liquid inside the unit may also result in performance loss Please discard tips after use Repeated use may result in loss of accuracy There is also danger of contamination caused by mixing of liquids Liquid remaining in a tip may be mixed with new liquid suctioned in causing an undesired result Do not turn the unit on its side or upside down when there is liquid in the tips Liquid will get inside the unit which may result in performance loss After autoclaving and drying wait till the unit has cooled down sufficiently before using Using while warm may result in loss of accuracy After autoclaving and drying assemble the unit after it has cooled down sufficiently Assembling while warm may result in damage to screw heads or other forms of product quality degradation When turning the push button don t exceed the specified sample volume limit otherwise
8. 2 Replacement parts list Please specify volume and channel number when ordering Replacement part s name Contents size Ring x 1 common Connection washer x1 common Connection screw x 1 common Casing A x 1 Insert nut x 5 Casing A set BIE button 1 Each volume Ejector shaft A x 1 Ejector shaft B x 1 Ejector spring x 1 Casing B x 1 Each volume Plunger fixing unit x 1 First spring x 2 Guide shaft x 2 Plunger fixing unit set Guide tube x 2 Each volume E ring x 4 Bush x 1 Stem x 1 Plunger head spring Set 8ch x 8 12ch x 12 common Plunger set unge BenX zen Te Each volume Plunger head 8ch x 8 12ch x 12 X ring suppressor set X ring suppressor 8ch x 2 12ch x 3 Each volume Nozzle spring holder set Nozzle spring holder 8ch x 2 12ch x 3 common X ring set X ring 8ch x 8 12ch x 12 Each volume Nozzle set Nozzle 8ch x 8 12ch x 12 Each volume Nozzle spring set Nozzle spring 8ch x 8 12ch x 12 common Casing holding screw x 4 common Ejector plate x 1 Ejector set Flat washer x 1 Each volume Ejector plate holding screw x 1 Wrench x 1 common Note Parts marked with a star xx influence precision of the product When replacing these parts please replace all of them with new parts Xx Please contact your local distributor for price information Xx Specifications of the instruments and optio
9. France Belgique Belgi Fisher t l 0388671414 tel 056 260 260 Bioblock Scientific 0388671168 fax 056260270 email infos bioblock fr email belgium bioblock com Parc d innovation BP 50111 F67403 illkirch cedex www bioblock com www bioblock be Mode d emploi Espa a tfno 915159234 fax 91 515 92 35 email ventas bioblock com www es fishersci com Autoclavable N i C h I pe t 7000 Multi channel digital micro pipette User s manual CERTIFIED 1509001 Thank you for your purchase of our Nichipet 7000 For safe and proper use of the Nichipet 7000 please be sure to carefully read this manual After reading this manual please keep it in a convenient place for quick reference BE ORIGINATIVE Always Pursuing Originality NICHIRY Since 1944 Autoclavable N C h I pet 0 00 Multi channel digital micro pipette Features Complete autoclaving 121 C 20 minutes is possible with this unit Fatigue free operation for long time Digital system allows easy one touch volume setting One touch locking function with single hand Covering 0 5 u to 300 u range with 4 models each of 8 and 12 channels Unit construction design helps to prevent accuracy change due to hand temperature Suitable for sampling to a micro plate with 96 well and 9 mm pitch Because the handle and casing angle can be adjusted freely 360 degrees the unit can be used at a position of your choice includes
10. ay cause an accident Do not use for handling of liquids that will be directly injected into the body As certain types of liquids are harmful to the body never discharge any sample liquid while pointing the instrument at a person Do not eject tips while this unit is pointed at a person Do not eject tips when there is liquid inside The sharp protruding tips of the unit are dangerous Please use care when handling Please make sure that the tips are securely attached to the nozzle Scattering of liquid will occur if tips fall off the nozzle If liquid that is harmful to the body has splashed onto the unit treat it properly before using it When using liquid that is harmful to the body never touch a tip that has been used Do not use this unit to mix liquid etc This will cause tips to loosen fall off or result in liquid splashing on the unit After autoclaving or drying do not touch the unit as it is very hot Touching the unit could result in bodily harm Although this product is designed to resist chemicals it may be damaged by some kinds of chemicals such as N methyl pyrrolidinone etc Please contact the manufacturer before using special chemicals 10 Matters that require strict observance Users are required to strictly observe the following points in order for the instrument to keep its excellent precision reproducibility and original performance for a long time Avoid exposing this unit
11. g 5 Autoclaving Autoclaving of this complete unit is possible Follow the procedure below at atemperature of 121 C for 20 minutes 1 Be sure to loosen the lock handle and set the counter to the maximum volume number of the volume range Fig A 2 Loosen a connecting screw in the Fig 2 by 180 degree 3 Loosen four screws on the casing in the Fig 4 by 180 degree 4 When placing inside the autoclaving unit be careful not to damage the nozzle Also place in a position that does not put any weight on the nozzle 5 After autoclaving be sure to dry the unit thoroughly Drying Carry out drying immediately after autoclaving Dry the unit thoroughly in a constant tempera ture air drier at 60 C for over 60 minutes 1 Dry the product in the same condition as when it was autoclaved 2 Be careful not to damage the nozzle when placing the unit in the dryer Also make sure that the unit is placed in a position that does not place any weight on the nozzle 3 Make sure that the unit turned to the normal temperature after the drying Tighten the connecting screw in the Fig 2 and four screws on the casing in the Fig 4 And make sure that a ring in the Fig 1 is tightened Note After drying if the unit is assembled while it is still warm screw head damage etc may occur causing damage to the product or loss of product performance Be sure to reassemble the unit after it has completely cooled down Also using the product while it is warm
12. nal accessories as well as contents of accessory sets are subject to change without notice x No part of this manual may be reproduced reprinted in any form without permission of the copyright holder This is strictly prohibited by the copyright law 13 Memo NICHIRYO 4 10 lwamoto cho 2 chome Chiyoda ku Tokyo 101 0032 Japan TEL 81 3 5820 2664 FAX 81 3 5820 2410 Homepage URL http www nichiryo co jp E mail info nichiryo co jp E 01 2004 01 1000
13. ng it counter clockwise and stop when the oval opening on the lock handle faces you Fig 7 3 Rotate the push button until one of two hex head screws can be seen through the oval opening Fig 7 4 Loosen both hex head screws with a hex head wrench 1 5 mm by turning them counter clockwise one by one Fig 8 5 Keeping the hex head wrench inserted into one hex head serew turn the push button to calibrate the pipette Fig 8 6 The pipetting volume can be adjusted by rotating the push button clockwise to in crease and counter clockwise to decrease Please refer to the table standard volume adjustments Degree of Rotation 0 5u 10u 5u 50u 40u 200u 4 5040 300p 2 360 0 31 u 2 1 6 u 2 6 5 u l 9 6 u l 720 0 63 u 2 3 2 u 2 13u 19 2 u 4 7 Tighten the both hex head screws after ad justing the push button and measure the accuracy of the pipette 8 Repeat the above procedures until the pi pette is calibrated within the specified ac curacy An accuracy test should be made SER at the specified minimum and maximum volume of each pipette 9 Return the cap to its original position E Push button Lock handle Oval opening 4 gt Hex head screw Fig 7 TD Increase g B Decrease Hex head wrench 11 Troubleshooting Problem Probable cause Solution f Foreign matter in the Remove foreign matter with
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