Home

FractionCollectorFrac-920and Frac-950

1. Free Methods 99 Method Occupied Sel Method free 01 edit edit clear Set Fraction Size Clear Method 01 00 0 ml yes yes no Set Fraction Start 0 ml Set Method End 0 ml Set Drop Sync Active no Set Peak Detect Sel Peak Detect Slope Level Slope Off Save Method Set Slope yes yes no 0 min 0 _ Set Peak Frac Size 0 00 ml 000 _ Note Before starting to create a method the method base should be checked for more information refer to Fraction Collector Frac 920 User Manual Creating a new method To create a new method From the Run Method menu press V to reach the Program Method menu Program Method 2 Press OK 3 The number of free methods is displayed Free Methods 99 Sel Method free 01 4 Select a free method and press OK Editing a method To edit a method Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 4 Operation 4 1 Fraction Collector Frac 920 stand alone 32 1 Select a method number to edit Press OK Method Occupied edit edit clear 2 Select edit in the Method Occupied menu Press OK Note It is only possible to change methods that have the same method base as the one currently selected in Setup and Check Clearing a method To clear a method independent of method base 1 Select a method number to clear Press OK Me
2. In order to maintain the declared EFUP the product shall be operated normally ac cording to the instructions and environmental conditions as defined in the product manual and periodic maintenance schedules specified in Product Maintenance Pro cedures shall be followed strictly Consumables or certain parts may have their own label with an EFUP value less than the product Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures This product must not be disposed of as unsorted municipal waste and must be collected separately and handled properly after decommissioning Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 23 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS ABASURARAANAMREE Name and Concentration of Hazardous Substances PRUBEBEU SUISSE Table of Hazardous Substances Name and Concentration BBE S A LS ETC EE r p Component name Hazardous substance is Ed E 2 RRA Z0R EE Pb Hg Cd 18 1177 40 X 0 0 0 0 0 18 6083 00 X 0 0 0 0 0 0 RRBZASAS WAREZRBEM ARMA HS 8139165J 111363 2006 tr EME B BR BE RAE X An VUB S8 UR ze IP TE RAE AR HA EHH E EE 08 5 111363 2006 HEM EMRBER Js PIS A A RT PIT BEDA SY REA 0 Indicates that this toxic or hazardous substance contained in all of the homogeneous ma terials for this part is below the limit requirement in SJ T11363 2006
3. x Indicates that this toxic or hazardous substance contained in at least one of the homoge neous materials used for this part is above the limit requirement in SJ T11363 2006 e Data listed in the table represents best information available at the time of publication 24 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 3 Installation 5 Installation Fraction Collector Frac 920 and Frac 950 is delivered in protective packing material and shall be unpacked with great care Any equipment connected to Fraction Collector Frac 920 and Frac 950 must fulfill appli cable standards and local regulations For detailed information on Installation see Fraction Collector Frac 920 and Frac 950 User Manuals 5 1 Site requirements Parameter Requirement Operation site Indoor use Altitude Maximum 2000 m Electrical power 100 240 V AC 10 50 60 Hz Transient overvoltages Overvoltage category Il Ambient temperature 4 C to 40 C Placement Stable laboratory bench Humidity 20 to 95 non condensing Pollution degree 2 5 2 Transport The equipment can be transported on a trolley capable of supporting at least 20 kg NOTICE e Always lift Fraction Collector Frac 920 and Frac 950 by the base unit never by the delivery arm as this may damage the arm Before moving the system disconnect all cables and tubing connected to peripheral components and liquid containers Frac
4. 2 Press A or V to change the set value A cursor below a text or numerical value shows what is affected when pressing the buttons CN Control buttons Press OK to verify the set value and exit the set value mode To cancel press Esc Button Description feed Press feed tube to advance the fraction collector one position The tube feed is delayed according to the set value in the Set Delay UV to Frac menu The control button feed tube is disabled if connected to CAN bus If the pump is off the tube feed is done without a delay Press end to interrupt method operation before the method is completed pump on off Press pump on off to start stop the pump When no method is running the pump can be used for e g buffer exchange or equilibration pause cont ct c D Press pause cont to pause all operation without ending the method All functions including pump and fraction collector are stopped Press pause cont again to restart the method operation 30 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone Programming a method Under this menu you can create up to 99 different methods In a method you can choose e g fraction size and fraction start and stop When saving a method it will be designated a method number Program Method
5. 61010 1 EN 61326 1 Electrical equipment for measure EN standard is harmonized ment control and laboratory use with EU directive EMC requirements 2004 108 EC EN ISO 12100 Safety of machinery General EN ISO standard is harmo principles for design Risk assess nized with EU directive ment and risk reduction 2006 42 EC FCC compliance This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation Note The user is cautioned that any changes or modifications not expressly approved by GE Healthcare could void the user s authority to operate the equipment This equipment has been tested and found to comply with the limits for a Class B digital device pursuant to part 15 of the FCC Rules These limits are designed to provide rea sonable protection against harmful interference in a residential installation This equip ment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the eq
6. 920 and Frac 950 Operating Instructions 28 9622 09 AC 25 3 Installation 3 2 Transport e lift the instrument by the base unit To make it easier to move Fraction Collector Frac 950 on the laboratory bench first lift the front approx 30 and tilt the unit until the rubber feet clear the bench Then move the unit to the desired location 5 5 Unpacking Check for damage Check the equipment for damage before starting assembly and installation There are no loose parts in the transport box All parts are either mounted on the system or located in the accessory kit box If any damage is found document the damage and contact your local GE Healthcare representative Unpack the system Remove straps and packing material Then set the equipment upright before starting installation 5 4 Assembly The following parts must be added to the Fraction Collector Frac 920 and Frac 950 in strument before it can be used e Tube rack e Collection tubes Tubing Waste tube 5 5 Connections Communication Make sure that UNICORN control software is installed on the computer Connect the Fraction Collector Frac 920 and Frac 950 according to the electrical drawings in electrical and communication connections for Fraction Collector Frac 920 or electrical and communication connections for Fraction Collector Frac 950 respectively 26 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 3 Installation 3 5 Connections F
7. Press OK 35 Select which method to run Select method to Run Method 1 1 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 35 4 Operation 4 1 Fraction Collector Frac 920 stand alone 4 Press OK Press OK to start 5 Press OK to start the run The status of the run is displayed By using the arrow buttons different statuses are displayed Run menu 1 2 3 1 1 RUN 10 3 ml Tube 03 3 08 5 00 ml 4 5 No Description 1 Method no 2 Method status 3 Method run length 4 Tube no 5 Actual tube volume fraction or peak fraction size Tube volume is counted from 0 to set value Run menu 2 1 2 10 0 ml min No Peak 22 FSD 0 min Description 1 Flow ml min if a valid pump is connected 36 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone No Description 2 Peak indication Peak or No peak is shown if peak detect is turned on Slope or Level 3 UV absorbance value FSD 4 Slope or level value min or 96 Fraction size Method End and Peak detect can be changed during the run To reach the menus use the arrow button The changes of settings are the same as for program ming a method By setting the value of fraction size to zero the SV 923 valve is set to waste if connected Pause cont can be activated Method End cannot be set to a value be
8. long delay time Tubes skipped Faulty parameters in UNICORN may be the cause Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 49 6 Troubleshooting 6 1 Faults and actions Fault Action DropSync is not func e The drop sensor photocell is dirty Clean the photocell tioning with a damp cloth e Check that the capillary end projection is not too long 2 mm e Check that the flow rate is not too high a continuous flow No fractions are collect Check that the safety bar is folded up ed Liquid misses the tubes e Check that the DropSync unit is close enough over the tubes e Check that the rack is correctly fitted to the rack holder e Check thatthe capillary end is cut cleanly and straight e Check that the correct rack type is selected 50 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 7 Reference information 7 Reference information About this chapter This chapter contains technical data regulatory and other information 7 1 Specifications Parameter Value Ingression protection IP21 Frac 950 IP22 Frac 920 Supply voltage 100 240 V AC 10 50 to 60 Hz Power consumption 300 VA Frac 950 20 VA Frac 920 Dimensions H x W x D 480 x 380 x 550 mm Frac 950 250 x 320 x 400 mm Frac 920 Weight 16 5 kg Frac 950 4 5 kg Frac 920 Ambient temperature 4 to 40 C Relative humidity toleranc
9. 006 BFS ais HE SIESIRSER HRM TS X58 RASNE The following product pollution control information is provided according to SJ T11364 2006 Marking for Control of Pollution caused by Electronic Information Products BFE SP n5 RE TB zi H Explanation of Pollution Control Label YOBGSGRBRARIT me 8ESJ T11363 2006 HFRS mess xm BR Loy BER PRENASESYR MERAWRFAAT MAS FB RA FRELREAWRET ABASWAFASREARRS PHP IS HAST Bn TAPHAUG RUSjSxXGON EAR WIGYE REPUIEBHR HF 23 RAGE Pt FR BH E E 58 FA BBR ER Gn FE FP PE SR HE 0 75 HE fT 1E FEA APRETI TE PME RAE 48 AU RT EC TG AY aE A RU SE ES BF T BER HER BE 8 FB rs OF EL RUNS FB HBERCE RI RELL hE MASHER MEARS m 4E EET BIR ERAS ao EL UEPITFRER S SEI 77 in AR fe FR BR DR ANF Ge CEE FA dn As RAN AR RET SS EEA NIIS RS SA This symbol indicates the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Products The number in the symbol is the Environment friendly Use Period EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating con ditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year
10. 015 General Electric Company 2009 2015 General Electric Company All rights reserved First published Jul 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9622 09 AC 02 2015 a4
11. Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 39 4 Operation 4 3 Fraction Collector Frac 950 The green indicator shows when flashing power is on when continuously lit power is on and connection with UNICORN is established When lit the yellow indicator shows that Fraction Collector Frac 950 is running When connected to UNICORN several beeps are heard after which Fraction Collector Frac 950 starts to perform an automatic calibration process During this process the tube holder moves rapidly in several directions When calibration is completed the tube holder stops in the home position Note The safety bar must be folded up for Fraction Collector Frac 950 to start up Operating Fraction Collector Frac 950 40 Fraction Collector Frac 950 is controlled from a PC running UNICORN version 3 21 or higher for standard mode or version 4 0 or higher for prep mode For AKTAmicro UNICORN version 5 0 or higher is recommended Control of Fraction Collector Frac 950 can be achieved automatically from a method or manually via the functions available in UNICORN Using Fraction Collector Frac 950 in a method is described in the AKTA design Optional Configuration User Manual The following functions are available for operating Fraction Collector Frac 950 from UNICORN Manual Method Man_Fractionation Fractionation Fractionation_Stop Fractionation_Stop Feed_Tube Feed_Tube Man_Peak_Fractionation
12. Frac 920 can also be used as a stand alone unit 8 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 3 Instrument 1 3 1 Fraction Collector Frac 920 1 3 1 Fraction Collector Frac 920 Main parts SS SIs SEES 5 Table 1 1 The main parts of Fraction Collector Frac 920 Part Description 1 Delivery arm 2 Lock knob 5 Control buttons 4 Control buttons 5 Collection tubes 6 Tube rack 7 Programming buttons 8 Menu display Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 9 1 Introduction 1 3 Instrument 1 3 1 Fraction Collector Frac 920 Electrical and communication connections 1 2 3 4 9 6 Counter Vie EJ Remote Uninet 1 1 Drop sensor 2 Valve 3 Auxillary equipment 4 Remote digital I O 5 UniNet 1 6 Power inlet 10 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 3 Instrument 1 3 2 Fraction Collector Frac 950 1 3 2 Fraction Collector Frac 950 Main parts FRAC 950 Table 1 2 The main part of Fraction Collector Frac 950 Part Description 1 Delivery arm 2 Safety bar 3 Collection tubes 4 Tube rack 5 Indicators Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 11 1 Introduction 1 3 Instrument 1 3 2 Fraction Collector Frac 950 Electrica
13. Fraction Collector Frac 920 and Frac 950 Operating Instructions Original instructions Table of Contents Table of Contents 1 Introd UE OR cascade bend it hut mf dera t c LUE 3 11 Important user information ettet 4 12 Regulatory information 5 1 5 IriStr riie Fit soiree 8 1 5 1 Fraction Collector Frac 920 T 9 1 5 2 Fraction Collector Frac 950 ttti tttttcccstnn 11 T4 CORHOL SONO reete ai radit resorte inest eet hd ier Mee 12 15 Us rdocumerntatloh ccrte iore tinte tine etii i e ERA 12 Safety INStrUCtIONS M 14 21 Safety precautions conet re ect o RE ERE ERE 14 LOEMENNKI em 19 2 3 Emergency pFocedul s uices butter le mte o tu cer bebo 21 2 4 Recycling AntOr dtr einean e Pee RR ere pret Mae meti terit 22 25 Declaration of Hazardous Substances DOHS eet 23 Tabycel ltsleic 7 Reereeereneeeer erp erere NIU MI UI 25 3 1 Site requirements ou eeecceecsssssesssssseesccsssescsssuessssseecsssssccsssnsccssssscesssssecsesssecssssscceccsscsesssscecsssseecessseeesesiee 25 3 2 MEDS ID OM p M 25 3 5 Unpacking 26 5 5 JASSSmblVisseneuiitsenee utate tege ns ameet e P eee E LESE E 26 3 5 CONNECTIONS x 26 3 6 Spareparts and accessories oer RUE ER RR RE EEUU 27 lg M 28 41 Fraction Collector Frac 920 stand alone entente 28 4 2 Fraction Co
14. Peak_Fractionation Peak_Frac_Stop Peak_Frac_Stop AccumulatorWash AccumulatorWash Ignore_LastTube Reset_Frac_Position Reset_Frac_Position PeakFrac Parameters PeakFrac Parameters Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 3 Fraction Collector Frac 950 WINCORN LAB14NT Frac Instructions Instructions r Parameters C Pump FractionationStop neck C Flowpath FeedTube 18 amp 30 mmTubes 1 Man_Peak_Fractionation AlamskMon Peak_FracStop Frac order G Fad Peak_FracParamet tes Acoumulatotwash 2 C Other Ignore_LastTube zl 0 000 33333 933 H mi 4 No Description No Description 1 Select rack types 3 Select tube type and size 2 Select fractionation order 4 Select tube type and size m ee eee ia Pump FractionationStop C Flowpath FeedTube f Er 1 O Et 2 C Tie 6 vame 2 C Other Ignore_LastTube Description b Description 1 Select starting tubes 2 Select fractionation bases Itis also possible to set the delay volume i e the added volume of tubing and components between the UV flow cell in the AKTA system and Fraction Collector Frac 950 This value must be changed when the AKTA standard configuration system is changed to an op tional configuration Collecting fixed fractions Details about collecting fixed fraction volumes using UNICORN in a method are described in the AKTA des
15. RN LAB14NT Frac Instructions x Instructions Parameters Man Fractionation Insert C Pump FractionationStop C Flowpath peels an Peak Fractionation AlarmstMon Peak_FracStop Frac Peak_FracParameters Accumulatorwash Other 3 Click on the Execute button 4 Fill up the rack with new tubes 42 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 3 Fraction Collector Frac 950 5 Click on the Continue button to restart the fractionation in the next tube Reset_Frac_Position The instruction Reset_Frac_Position resets the fraction collector This means that frac tionation set to start at next position will start in the first position The instruction will reset next position for all tube types 1 Select menu System Control Manual Frac in UNICORN 2 Select the instruction Reset Frac Position in the Frac list 3 WINCORN LAB14NT Frac Instructions Instructions Parameters Insert FractionationStop c Duro FeedTube C Flowpath Man Peak Fractionation Peak FracStop C AlarmstMon Peak FracParameters Frac AccumulatotW ash Ignore LastTube C Other Click on the Execute button AccumulatorWash The accumulator used to eliminate spillage at tube change can be manually washed with the instruction AccumulatorWash 1 2 Start a flow of 10 ml min manually using the system pump If the fraction collector is connected to any other port than
16. T Specials Instructions x Instructions FracParameters Parameters Abas FracParameters DelayVol 10 10 r TubeChange DropSync TEE zi ml Specials Peak_FracParameters StartSlope 100 000 mAU min TubeChange C EndSlope 75 000 m amp U min Monitore Miriwicth 0 150 min DropSync I Keyboard Mode Open C Curves Set Selected Parameter To Strategy Default Value Cancel Help 4 The appropriate value for your KTA system is found in the KTA design Optional Configuration User Manual 5 Click on the OK button The value entered will be used until a further change is made Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC Flow control during tube change 4 Operation 4 3 Fraction Collector Frac 950 The liquid flow during tube change can be handled in three different ways 1 In UNICORN select menu System Control System Settings 2 Clickthe Specials radio button and select instruction FracParameters Highlight the TubeChange instruction WINCORN LAB2 NT Specials Instructions x Instructions FracParameters Parameters C FracParameters DelayVol 0 10 fem DelayVol 0 375 ml pos TubeChange DropSync m Specials Peak FracParameters StartSlope 100 000 mALI min TubeChange 3 EndSlope 75 000 m amp U min LEO Minit 0 150 min pou a Keyboard Tube Mode Open DropSync C Curves LN Set Selected Parameter To Strategy Default Value Cancel Heb 3 For the parameter TubeChange select one of the following o
17. able protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of Fraction Collector Frac 920 and Frac 950 The fraction collector should be kept clean and spilled liquid should be wiped off before it dries Remove dirt from the surface using a cloth and a mild cleaning agent The rack holder should be positioned over the centre The safety bar should be folded down when the fraction collector is not in use Fraction Collector Frac 950 only When your AKTA system is cleaned also clean the capillaries and the accumulator in Fraction Collector Frac 950 with distilled water The instrument should be wiped regularly with a damp cloth Remember to wipe the DropSync unit photocell as well Allow the instrument to dry completely before use Cleaning before planned maintenance service To ensure the protection and safety of service personnel all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts maintenance work Please complete the checklist in the On Site Service Health and Safety Declaration Form or the Health and Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from Section 7 2 Health and Safety Declaration Form on pag
18. ately in an undefined state Frac 920 and Frac 950 Computer e The UNICORN computer shuts down in an undefined state e The data collected up to the time of the power failure is available in UNICORN Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 21 2 Safety instructions 2 4 Recycling information 2 4 Recycling information Decontamination Fraction Collector Frac 920 and Frac 950 shall be decontaminated before decommis sioning and all local regulations shall be followed with regard to scrapping of the equipment Disposal general instructions When taking Fraction Collector Frac 920 and Frac 950 out of service the different ma terials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances Fraction Collector Frac 920 and Frac 950 contains hazardous substances Detailed in formation is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment 22 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS 25 Declaration of Hazardous Substances DoHS 383 5J 111364 2
19. ation Manufacturing information The table below summarizes the required manufacturing information For further infor mation see the EU Declaration of Conformity DoC document Requirement Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Conformity with EU Directives This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards Directive Title 2006 42 EC Machinery Directive MD 2004 108 EC Electromagnetic Compatibility EMC Directive 2006 95 EC Low Voltage Directive LVD CE marking CE The CE marking and the corresponding EU Declaration of Conformity is valid for the in strument when it is e used as a stand alone unit or e connected to other products recommended or described in the user documentation and e usedinthe same state as it was delivered from GE Healthcare except for alterations described in the user documentation 6 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 2 Regulatory information International standards This product fulfills the requirements of the following standards Standard Description Notes EN IEC 61010 1 Safety requirements for electrical EN standard is harmonized UL 61010 1 equipment for measurement with EU directive CAN CSA C22 2 control and laboratory use 2006 95 EC No
20. biological agents The operator has to take all necessary actions to avoid spreading hazardous biological agents in the vicinity of the instrument The facility should comply with the na tional code of practice for biosafety Installing and moving the instrument WARNING Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the equipment before con necting the power cord WARNING Fraction Collector Frac 920 and Frac 950 must always be connect ed to a grounded power outlet WARNING Power cord Only use power cords with approved plugs delivered or approved by GE Healthcare WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect gt Pe gt Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 17 2 Safety instructions 2 1 Safety precautions NOTICE Any computer used with the equipment shall comply with IEC 60950 and be installed and used according to the manufacturer s instruc tions System operation WARNING Hazardous chemicals during run When using hazardous chemi cals run System CIP and Column CIP to flush the entire system tubing with distilled water before service and maintenance WARNING Hazardous biological agents dur
21. d by mains voltage and handles liquids that may be hazardous Before installing operating or maintaining the system you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injury or damage to the equipment The safety precautions in this section are grouped into the following categories General precautions Using flammable liquids Personal protection Installing and moving the instrument System operation Maintenance General precautions Always follow these General precautions to avoid injury when using Fraction Collector Frac 920 and Frac 950 WARNING Do not operate Fraction Collector Frac 920 and Frac 950 in any other way than described in the Fraction Collector Frac 920 and Frac 950 Operating Instructions 14 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 2 Safety instructions 2 1 Safety precautions WARNING Operation and user maintenance of the Fraction Collector Frac 920 and Frac 950 should be performed by properly trained personnel only WARNING Do not use any accessories not supplied or recommended by GE Healthcare WARNING Do not use the Fraction Collector Frac 920 and Frac 950 if it is not working properly nor if it has suffered any damage for example damage to the power cord or its plug e damage caused by dropping the equipment e damage caused by splashing liquid onto it WARNING Operat
22. e 20 to 95 non condensing Atmospheric pressure 84 to 106 kPa 840 to 1060 mbar Acoustic noise level 73 dBA Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 51 7 Reference information 7 2 Health and Safety Declaration Form 7 2 Health and Safety Declaration Form On site service On Site Service Health amp Safety Declaration Form Service Ticket To make the mutual protection and safety of GE service personnel and our customers all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair To avoid delays in the servicing of your equipment please complete this checklist and present it to the Service Engineer upon arrival Equipment and or work areas not sufficiently cleaned accessible and safe for an engineer may lead to delays in servicing the equipment and could be subject to additional charges Please review the actions below and answer Yes or No Provide explanation for any No answers in box below Instrument has been cleaned of hazardous substances Please rinse tubing or piping wipe down scanner surfaces or otherwise ensure removal of any dangerous residue Ensure the area around the instrument is clean If radioactivity has been used please perform a wipe test or other suitable survey Adequate space and clearance is provided to allow safe access for instrument service r
23. e 52 or print it from the PDF file available on the User Documentation CD Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 47 5 Maintenance 5 3 Changing capillaries Do 5 4 48 Changing capillaries Change the capillaries when they show signs of leakage or wear sharp bending for example Changing waste tubing Change the waste tubing when it shows signs of wear Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 6 Troubleshooting 6 Troubleshooting A WARNING Disconnect power Always disconnect power from the instrument before performing any maintenance task WARNING Do not open the instrument There are no user serviceable compo nents inside and you can be exposed to high voltage CAUTION Be sure to fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced Fraction Collector Frac 950 only gt Pb CAUTION Only spare parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the unit 6 1 Faults and actions Fault Action No tube change e Start a flow and start fractionation e Select FeedTube from the menu SystemControl Man ual Flowpath If the motor does not start and an error appears contact GE Healthcare e Check the delay volume A large delay volume at a low flow rate generates a
24. e attached to the Fraction Collector Frac 920 and Frac 950 instrument For infor mation about marking of the computer equipment refer to the manufacturer s instruc tions Labels on the instrument The illustration below shows an example of the identification label that is attached to Fraction Collector Frac 920 and Frac 950 XX XXXX XX XXXXX Samp le Code no XXXXXXXX Voltage IXI XXX XXX XXX VAC Serial no XXXXXXX Frequency XX XX Hz Mfg Year 2014 Max Power XXX VA Fuse XX X XXAL 250 V a Protection Class XXXX Lisreo AE CC uo 4001767 IEEE GE Healthcare Bio Sciences AB f Made in Sweden 751 84 Uppsala Sweden B Conforms to ANSI UL Std 61010 1 Cert to CAN CSA Std C22 2 No 61010 1 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 19 2 Safety instructions 2 2 Labels Symbols used in instrument Label Meaning labels Warning Read the user documentation before using the equipment Do not open any covers or replace parts unless specifically stated in the user documentation The equipment complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand The equipment complies with applicable European directives q has been certified by a Nationally Recognized Testing Laboratory c us This symbol indicates that Fraction Collector Frac 920 and Frac 950 NRTL NRTL means an organization which is recognized by the US Occupational Safety a
25. efinitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood 4 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 1 Important user information Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE e NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A note is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information In this section This section describes the directives and standards that are fulfilled by Fraction Collector Frac 920 and Frac 950 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 5 1 Introduction 1 2 Regulatory inform
26. epair or installation In some cases this may require customer to move equipment from normal operating location prior to GE arrival Consumables such as columns or gels have been removed or isolated from the instrument and from any area that may impede access to the instrument All buffer waste vessels are labeled Excess containers have been removed from the area to provide access Provide explanation for any No answers here Equipment type Product No Serial No hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the area has been made safe and accessible Name Company or institution Position or job title Date YYYY MM DD Signed GE and GE monogram are trademarks of General Electric Company GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 2010 14 General Electric Company All rights reserved First published April 2010 DOC1149542 28 9800 26 AC 05 2014 52 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC Product return or servicing 7 Reference information 7 2 Health and Safety Declaration Form Health amp Safety Declaration Form for Product Return or Servicing Return authorization number and or Service Ticket Request To make sure the mutual protection and safety of GE personnel our cust
27. ign Optional Configuration User Manual Collecting peak fractions Details about collecting peaks only using Fraction Collector Frac 950 in a method are described in the AKTA design Optional Configuration User Manual Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 41 4 Operation 4 3 Fraction Collector Frac 950 Feed tube During fractionation the tube rack can be moved forward one tube with the instruction FeedTube 1 Select menu System Control Manual Frac in UNICORN 2 Select the instruction FeedTube in the Frac list WINCORN LAB14NT Frac Instructions x Instructions Parameters IER Man Fractionation 1 C Li RO FractionationStor C Flowpath qma an Peak Fractionation C AlarmstMon Peak FracStop Frac Peak FracParameters Accumulator Wash Close C Other Ignore_LastTube _ Help 3 Click on the Execute button The tube rack moves on to the next tube after the set delay volume has been collected Ignore LastTube The tube position in the rack which is defined in the start protocol to have the last tube can be ignored with the instruction Ignore LastTube When the last tube is reached and there are more fractions to collect an alarm is gen erated and the system is paused You can then fill up with new tubes and use the instruc tion Ignore LastTube 1 Select menu System Control Manual Frac in UNICORN 2 Selectthe instruction Ignore LastTube in the Frac list WINCO
28. ing run When using hazardous biological agents run System CIP and Column CIP to flush the entire system tubing with bacteriostatic solution e g NaOH fol lowed by a neutral buffer and finally distilled water before service and maintenance CAUTION Pinch hazard Do not have any part of your body within the unit base area when Fraction Collector Frac 950 is switched on An automatic calibration process starts when Fraction Collector Frac 950 is connected to UNICORN During calibration the dis penser arm moves rapidly Several beeps are heard before the calibration procedure starts gt P gt gt CAUTION Be sure to fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced Fraction Collector Frac 950 only 18 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 2 Safety instructions 2 1 Safety precautions Maintenance WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the user documenta tion WARNING Disconnect power Always disconnect power from the instrument before performing any maintenance task 2 2 Labels In this section This section describes the instrument labels and labels concerning hazardous substances that ar
29. ion and user maintenance of Fraction Collector Frac 920 and Frac 950 should be performed by properly trained personnel only WARNING Do not use the Fraction Collector Frac 950 if the safety bar is bro ken CAUTION Waste tubes and containers must be secured and sealed to prevent accidental spillage Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 15 2 Safety instructions 2 1 Safety precautions CAUTION Make sure waste container is dimensioned for maximum possible volume when the equipment is left unattended NOTICE Avoid condensation by letting the unit equilibrate to ambient tem perature Using flammable liquids WARNING A fume hood or similar ventilation system shall be installed when flammable or noxious substances are used Personal protection WARNING Always use appropriate personal protective equipment during operation and maintenance of Fraction Collector Frac 920 and Frac 950 WARNING When using hazardous chemical and biological agents take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of Fraction Collector Frac 920 and Frac 950 16 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 2 Safety instructions 2 1 Safety precautions WARNING Spread of
30. ion number and a beep is heard Frac 920 V2 00 If an error is detected during the self test an error message is shown 3 Theself test takes about five seconds When the self test is completed the display shows the Run Method menu Run Method 4 When UNICORN connects to Fraction Collector Frac 920 the displays shows the message Uninet 1 connected for for a few seconds Uninet connected 5 When connection is established the message Setup and check is displayed Setup and Check Flow during tube change The sample flow during tube change can be handled in two different ways The selections can be found under menu System Control System Settings Specials 1 Tochange the settings select the instruction FracParameters 2 For the parameter TubeChange select one of the following options e Tube No synchronization of collection e DropSync Tube change synchronized to drop release to minimise spillage Should only be used at flow rates below 5 ml min 3 Click on the Execute or OK button 38 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC Setting the delay volume 4 Operation 4 2 Fraction Collector Frac 920 connected to an AKTA instrument The delay volume between the UV monitor and Fraction Collector Frac 920 in your AKTA chromatography system can be set The fraction marks shown in UNICORN will be ad justed according to this volume to show the actual par
31. l and communication connections s z 5 1 UniNet 3 UniNet 1 Te 1001207 4 o Jozzo2 o v J ns mE 2 3 1 UniNet 3 connectors 2 DropSync connector 3 Power inlet 4 Power switch 5 UniNet 1 connector 14 Control software UNICORN control software UNICORN is a complete software for control and supervision of Fraction Collector Frac 920 and Frac 950 The software runs under Microsoft Windows operating system For more information about UNICORN control system see the UNICORN user manuals supplied 15 User documentation In addition to these Operating Instructions the documentation package supplied with Fraction Collector Frac 920 and Frac 950 also includes product documentation binders containing detailed specifications and traceability documents The most important documents in the document package with regard to technical aspects of Fraction Collector Frac 920 and Frac 950 are 12 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 5 User documentation System specific documentation User documentation Content Fraction Collector Frac 920 and All instructions needed to operate the instrument Frac 950 Operating Instructions in a safe way including brief system description installation and maintenance Fraction Collector Frac 920 and Detailed system description Comprehensive user Frac 950 Use
32. llector Frac 920 connected to an KTA instrument 37 AS Fraction Colector Frac 950 oet ecce tra pe HE E 39 Mainteliuli6e esee a e ad POE HO OE red v nd ERR OREL ERE ERE 47 5 1 J General x 47 5 2 Gleanirig zs 5 47 55 Changing Capilla ES uses ntn rd te Tn 48 52 Changing WASTE tubing eire tree ect er rer 48 WOUDIESNO OHN Giessen oer b D HERE ue o e mtd rr fF rad 49 6 1 Faults dnd actions tttm tiet t E ERREUR HE TUER ERE RR EE RH 49 Reference information as 51 Zl SpSCIGGLlOlS ucciso ar EE E EE raran SETIR EE putent Dre ten 51 7 2 Health and Safety Declaration Form wsccceccssssssssssssssssssscssssssssssusssssessccssssssssussssssessessssssssssnsseesees 52 43 Ordering nforrmatiOra eret terere er e erben crest bete ers 54 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 Introduction Purpose of the Operating Instructions The Operating Instructions provide you with the instructions needed to handle Fraction Collector Frac 920 and Frac 950 in a safe way Prerequisites In order to operate Fraction Collector Frac 920 and Frac 950 as is intended the following pre requisites must be fulfilled e The user should have a general understanding of how a PC and the Microsoft Windows operating system works if a computer is used e The user must understand the concepts of liquid chromatography The user must read and understand the Safety Instructions in this manual Fraction Collector F
33. low actual value of run method The pump on off button is not active during run 42 Fraction Collector Frac 920 connected to an AKTA in strument Fraction Collector Frac 920 is controlled from a PC running UNICORN version 3 0 or higher Control of Fraction Collector Frac 920 can be achieved automatically from a preprepared method or manually via the functions available in UNICORN Note Fraction Collector Frac 920 are designated Frac 900 in UNICORN Note When Fraction Collector Frac 920 is controlled from UNICORN all menu push buttons are disabled The manual instructions in UNICORN allow the following operations Collecting a fixed volume in each tube e Collecting each peak in a separate tube e Feed tube move on to next tube It is also possible to set the delay volume which corresponds to the volume between the UV monitor and Fraction Collector Frac 920 This value must be changed if the tubing is changed to tubing of a different inner diameter see Setting the delay volume on page 39 Starting the instrument 1 Connectthe power cord to the mains power outlet The display lights up and the system immediately performs a self test Selftest Please wait Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 34 4 Operation 4 2 Fraction Collector Frac 920 connected to an AKTA instrument 2 During the self test several messages are shown on the display for example system name and software vers
34. low path Connect inlet and outlet tubing to the system See Fraction Collector Frac 920 and Frac 950 User Manuals Electrical power Connect the power cord to a grounded power outlet specified in Section 3 1 Site require ments on page 25 3 6 Spare parts and accessories For correct up to date information on spare parts and accessories visit www gelifesciences com AKTA Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 27 4 Operation 4 Operation About this chapter This chapter provides instructions for the use of Fraction Collector Frac 920 and Frac 950 44 Fraction Collector Frac 920 stand alone Starting the instrument 1 Connect the power cord to the mains power outlet The display lights up and the system immediately performs a self test Selftest Please wait 2 During the self test several messages are shown on the display for example system name and software version number and a beep is heard If an error is detected during the self test an error message is shown Frac 920 V2 00 3 The self test takes about five seconds When the start up is completed the display shows the Run Method menu Run Method Menu display See Fraction Collector Frac 920 for the layout of the Frac 920 front panel Press A or V to select a specific menu Press OK to enter a sub menu e Press Esc once to move back one menu level or repeatedly to return to the mai
35. n menu level 28 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone Main menus Sub menus Press A orV ou ok c Semena ESC Submenu 2 1 ESC Sub menu 2 1 1 Press A orV OK gt ESC Sub menu 2 3 1 Sub menu 2 3 2 The Fraction Collector Frac 920 software is divided into three main menus Menu Description Run Method is used for running methods that are pro grammed by the user The Run Method menu is dis played after the self test See Running a method on page 35 Run Method Program Method is used for creating new methods To Program Method reach the Program Method menu press V See Pro gramming a method on page 51 Setup and Check is usedfor setup and checking internal system parameters such as Fraction Base and Delay UV to Frac For more information refer to Fraction col lector Frac 920 User Manual To reach the Setup and Setup and Check Check menu press V again Changing a parameter value 1 Set Fraction Size 1 0 ml 02 0 Description 1 Parameter Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 29 4 Operation 4 1 Fraction Collector Frac 920 stand alone N Description Current value WN New value to be set To change a parameter value 1 Press OK to enter the set value mode
36. nd Health Administration OSHA as meeting the legal requirements of Title 29 of the Code of Federal Regulations 29 CFR Part 1910 7 Labels concerning hazardous Label Meaning substances This symbol indicates that the waste of electrical and electronic equip ment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment 20 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 2 Safety instructions 2 2 Labels Meaning This symbol indicates that the product contains hazardous materials in tty excess of the limits established by the Chinese standard SJ T11363 2006 CO I Requirements for Concentration Limits for Certain Hazardous Substances in Electronics 2 5 Emergency procedures In this section This section describes how to do an emergency shutdown of the Fraction Collector Frac 920 and Frac 950 system The section also describes the result in the event of power failure Emergency shutdown In an emergency situation do as follows to stop the run Step Action 1 Press the Main power switch to the 0 position The run is interrupted imme diately Power failure The result of a power failure depends on which unit that is affected Power failure to will result in Fraction Collector e The run is interrupted immedi
37. ny or Name institution Position or job title Date YYYY MM DD Signed To receive a return authorization number or service number GE 2nd GE monogram are trademarks of General Electric Company please call local technical support or customer service GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 US 2010 14 General Electric Company All rights reserved First published April 2010 D0C1149544 28 9800 27 AC 05 2014 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 55 7 Reference information 7 3 Ordering information 7 5 Ordering information For ordering information visit www gelifesciences com AKTA 54 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA GE and GE monogram are trademarks of General Electric Company AKTA and UNICORN are trademarks of General Electric Company or one of its subsidiaries Microsoft and Windows are registered trademarks of Microsoft Corporation All other trademarks are the property of their respective owners Any use of UNICORN is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreement is available on request UNICORN 2003 2
38. omers transportation personnel and our environment all equipment must be clean and free of any hazardous contaminants before shipping to GE To avoid delays in the processing of your equipment please complete this checklist and include it with your return 1 Please note that items will NOT be accepted for servicing or return without this form 2 Equipment which is not sufficiently cleaned prior to return to GE may lead to delays in servicing the equipment and could be subject to additional charges 3 Visible contamination will be assumed hazardous and additional cleaning and decontamination charges will be applied Please specify if the equipment has been in contact with any of the following Radioactivity please specify Infectious or hazardous biological substances please specify Telephone No Other Hazardous Chemicals please specify Equipment must be decontaminated prior to service return Please provide a telephone number where GE can contact you for additional information concerning the system equipment Liquid and or gas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other please specify Equipment type Product No area has been made safe and accessible hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the Serial No Compa
39. port 1 in the Outlet valve you must manually switch Outlet Valve to the other port Select menu System Con trol Manual Flowpath in UNICORN Select the instruction OutletValve and select the desired port Click on the Execute button Select menu System Control Manual Frac in UNICORN Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 43 4 Operation 4 3 Fraction Collector Frac 950 6 Select the instruction AccumulatorWash in the Frac list WINCORN LAB14NT Frac Instructions x Instructions Parameters isa DEGR FrectonationStop Strokes 1 10 dee C Flowpath Man Peak Fractionation 5 m T OFF Peak FracStop AlarmstMon Peak FracParameters Frac M lgnore LastTube C Other Reset Frac Position Le 7 Select the number of strokes to be used for washing with the parameter Strokes 8 Click on the Execute button Setting delay volume 44 The delay volume between the UV flow cell in the chromatographic system and Fraction Collector Frac 950 must be known to UNICORN The delay volume is used to adapt the collected fractions to the event marks generated by UNICORN 1 In UNICORN select menu System Control System Settings 2 Click the Specials radio button and select instruction FracParameters The DelayVol instruction becomes highlighted 3 To change the setting click on the up and down arrows for the DelayVol parameter or type a new value directly in the parameter window WINCORN LAB2 N
40. ptions Tube No synchronization of collection Spillage will occur between tubes e DropSync Tube change is synchronized to drop release to minimize spillage Use i d 0 75 mm tubing between the DropSync and the accumulator Accumulator During tube change the flow is diverted to the accumulator which stores the liquid When the new tube is in position the liquid is pressed out rapidly for col lection 4 Flow rate limit recommendations using DropSync without spillage are given in the table below For higher flow rates the accumulator is recommended for spillage free fractionation For the 30 mm rack and Prep mode racks the accumulator is recom mended Rack type Flow rate limit using dropSync ml min microplates 0 to 1 0 12mm Oto 1 5 18 and 30 mm Oto 2 0 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 45 4 Operation 4 3 Fraction Collector Frac 950 5 After selection click on the OK button Define rack and tube parameters When running a method the rack and tube parameters for the rack to be used must be set This is described in detail in the AKTA design Optional Configuration User Manual 46 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 5 Maintenance 5 Maintenance 51 General Fraction Collector Frac 920 and Frac 950 require no periodic maintenance 5 2 Cleaning WARNING When using hazardous chemical and biological agents take all suit
41. r Manuals instructions method creation operation ad vanced maintenance and troubleshooting EU Declaration of Conformity for Document whereby the manufacturer ensures Fraction Collector Frac 920 and thatthe product satisfies and is in conformity with Frac 950 the essential requirements of the applicable direc tives Software documentation Together with each system the following software documentation is supplied providing additional information that applies to Fraction Collector Frac 920 and Frac 950 indepen dent of the specific configuration Document Purpose Contents UNICORN manual package e The manuals contain detailed instructions on how to administer UNICORN work with methods perform runs and evaluate results e The Online help contains dialog descriptions for UNICORN The Online help is accessed from the Help menu Component documentation Documentation for components produced both by GE Healthcare and by a third party are if existent also included in the document package Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 13 2 Safety instructions 2 Safety instructions About this chapter This chapter describes safety compliance safety labels general safety precautions emergency procedures power failure and recycling of Fraction Collector Frac 920 and Frac 950 2 1 Safety precautions Introduction Fraction Collector Frac 920 and Frac 950 are powere
42. rac 920 and Frac 950 and software should be installed configured and calibrated according to these Operating Instructions About this chapter This chapter contains important user information a description of the intended use of Fraction Collector Frac 920 and Frac 950 regulatory information list of associated documentation definitions of safety notices and so on Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 3 1 Introduction 1 1 Important user information 1 1 Important user information Read this before operating the product All users must read the entire Operating Instructions before installing operating or maintaining the product Always keep the Operating Instructions at hand when operating the product Do not operate the product in any other way than described in the user documentation If you do you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use Fraction Collector Frac 920 and Fraction Collector Frac 950 are automated fraction collectors for use in AKTA chromatography systems Fraction Collector Frac 920 can also be used as a stand alone unit Fraction Collector Frac 920 and Frac 950 are intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See d
43. ress OK Sel Peak Detect Level Slope Off 2 Select Peak Detect Level or Slope Press OK e f Level has been selected set an absorbance level 96 FSD when to start collecting fractions e If Slope has been selected set a slope min Set Slope 096 min 0 Press OK Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 33 4 Operation 4 1 Fraction Collector Frac 920 stand alone 34 3 Set peak fraction size ml Set Peak Frac Size 0 00 ml 0 00 4 Press OK For more information about the peak detection parameters refer to Fraction Collector Frac 920 User Manual Saving the method 1 Decide to save the method select yes or no Save Method yes yes no 2 Press OK Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone Running a method Used for running methods that are programmed by the user Run Method Select method to Run Method 1 1 Press OK to start 1 RUN 10 3 ml Tube 03 3 08 5 00 ml 10 0 ml min No Peak 2296 FSD 096 min Set Fraction Size 10 0 ml Set Method End 100 0 ml Set Peak Detect Slope Set Slope 096 min Set Peak Frac Size 0 00 ml Description 5 Description 1 Run menu 1 2 Run menu 2 1 Use the Esc and arrow buttons to reach the Run Method menu Run Method 2
44. thod Occupied edit edit clear 2 Select clear Clear Metod 01 yes yes no 3 To confirm the deletion select yes Press OK Setting fraction parameters When a new method is created or a current is edited the first displayed menu will be Set Fraction Size Set Fraction Size 00 0 ml 1 Press OK and select a fraction size using the arrow buttons Set Fraction Size 0 00 ml 10 0 2 Press OK To set fraction start press V Press OK 4 Setastart value Set Fraction Start 0 ml 10 0 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone 5 Press OK Note Fraction start is used if you want to have a delay between the method start and the start of collecting fractions The pump is always started di rectly at method start To set a method end press V Press OK 7 Seta method end value Set Method End 0 ml 100 8 Press OK To activate the drop synchronization press V Press OK Set Drop Sync Active no yes no 10 Select yes To deactivate the function select no Press OK Note The flow rate should be lt 5 ml min to make drop synchronization possible Setting peak detection The peak detecting function can be set to Off Level or Slope Set Peak Detect Off To skip peak detection press V 1 To activate a peak detecting function P
45. ts collected T 2 3 Select menu System Control System Settings Specials in UNICORN To change the settings select the instruction FracParameters Enter the new delay volume value The appropriate value for your particular system is found in the Reference information a chapter in the System Manual of your AKTA chromatography system Refer also to the AKTA design Optional Configuration User Manudl Click on the OK button The value entered will be used until a further change is made 4 5 Fraction Collector Frac 950 CAUTION Pinch hazard Do not have any part of your body within the unit base area when Fraction Collector Frac 950 is switched on An automatic calibration process starts when Fraction Collector Frac 950 is connected to UNICORN During calibration the dis penser arm moves rapidly Several beeps are heard before the calibration procedure starts CAUTION Be sure to fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced Fraction Collector Frac 950 only NOTICE The DropSync unit will be damaged if it is positioned below the tube rims Turn on the Fraction Collector Frac 950 using the Power switch at the rear panel The Fraction Collector Frac 950 now performs a self test There is one green and one yellow indicator on the front of Fraction Collector Frac 950
46. uipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different from that to which the receiver is connected Consult the dealer or an experienced radio TV technician for help Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 7 1 Introduction 1 2 Regulatory information Regulatory compliance of connected equipment Any equipment connected to Fraction Collector Frac 920 and Frac 950 should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within the EU connected equipment must be CE marked Environmental conformity This product conforms to the following environmental requirements Requirement Title 2011 65 EU Restriction of Hazardous Substances RoHS Directive 2012 19 EU Waste Electrical and Electronic Equipment WEEE Directive ACPEIP Administration on the Control of Pollution Caused by Electronic Information Products China Restriction of Hazardous Sub stances RoHS Regulation EC Registration Evaluation Authorization and restriction of No 1907 2006 CHemicals REACH 1 5 Instrument Fraction Collector Frac 920 and Frac 950 are automated fraction collectors for use in AKTA chromatography systems Fraction Collector

FractionCollectorFrac-920and Frac-950

Contents

Download Pdf Manuals

Related Search

Related Contents