HS 54 User Manual
Contents
1. P2 Vled 1 ca Vled 1 Vled 20 o Vled 2 o OutTT4_1 2 o OutTT10_1 2 o OutRef1 21 o OutRef2 21 o OutTT4_2 3 o OutTT10_2 3 Oo OutTT3_4 22 o OutTT9 4 22 o OutTT4 3 45 OutTT10_3 ae OutTT3_3 23 o OutTT9_3 23 o OutTT4 4 5 o OutTT10_4 5 o OutTT3_2 24 o OutTT9_2 24 o OutE1 elo OuteE3 ae OutTT3 1 25 a OutTT9_1 25 Stim2_Sre Tio Stim4_Ret fly VSS T vss 26 Stim2_Ret Bly Stim4_Sre Bly VDD 27 o VDD 27 o OutE2 9 o OutE4 9 Lo GndRef 28 o GndRef 28 o OutTT6_1 10 Lo OutTT12_ 1 10 o OutTT5 4 29 o OutTT11_4 29 o OutTT6_2 11 o OutTT12_2 11 o OutTT5_3 30 o OutTT11_3 30 o OutTT6_3 12 o OutTT12_3 12 o OutTT5_2 31 o OutTT11_2 31 o OutTT6 4 13 Lo OutTT12 4 13 o OutTT5_1 32 o OutTT11_1 32 o OutTT7_1 14 1G OutTT1_1 14 1 OutTT8_4 33 o OutTT2_4 33 o OutTT7 2 Bla OutTT1_2 15 OutTT8 3 al o OutTT2_3 a o OutTT7_3 16 o OutTT1_3 16 o OutTT8_2 35 o OutTT2_2 35 o OutTT7_4 17 o OutTT1_4 17 o OutTT8_1 36 o OutTT2_1 36 o Stim3_Src 18 o Stim1_Ret o i ig l Stim3_Ret 19 Stim1_Sre 19 o o T x7 CON uDB37 a CON uDB37 Tie gnds Tie gnds Figure 1 Pinout of the Two uDB37 Tether Connectors Note The ground signals on pin 37 and 28 are connected together on the headstage amplifier board Neuralynx Inc HS 54 User Manual Page 5 Revision 2 01 July 2009 5 Power Supply Requirements The Headstage Amp requires a dual supply 5v and 5v for the buffer Opamp amplifiers Current draw wil2. is labeled on each side of the respective tetrodes position The two Reference Electrodes adjustment locations are labeled as Ref1 and Ref2 8 LED s Power Usage and Customization Two separate conductors in each Tether Vled and Vled are used for LED power with the following benefits e The LEDs can be powered independently from the unity gain buffer amplifier circuitry e LED voltage may be different than the amplifier power voltages e Tether wire voltage drop is reduced since the LEDs will typically draw more current than the unity gain Opamp amplifiers The LED default pattern consists of single blue LEDs at the front and rear positions two red LEDs on the left and right adjacent positions of the front blue LED and two green LEDs on the left and right adjacent positions of the rear blue LED For this configuration the power supply voltage applied to the Vled and Vled tether connections should be 5v and 5v respectively Note These are the same voltages which are used for powering the amplifier circuitry and the same power supply may be used for both powering LEDs and the amplifiers There are a total of 14 LED mounting positions one between each Tetrode Adjustment Tool Alignment Cutout location Each of the 14 LED position consists of two surface mount LED mounting pads a total of 4 pads at each location on the top side of the board and 2 pads on the bottom side of the board for a single surface mounted LED serie
3. of Figures and Tables Figure Pinout of the Two uDB37 Tether Connectors eseesseeesesesesseressessrserersereresreeseeee 5 Fig re 2 Via Labeling om BIB suede os te ee ee eee 10 Table 1 HS 54 Channel Mapping eicics cayccooeies axccsapdeeegeeasduessconcagacs aid ecpdesndstas leelacteneeoeacys 10 Table 2 HS 54 Reference Mapping 4 4 1s accisassaesecsavenaceasnacaden snayaatasounctesnaveata soeesdeadooradees 10 Neuralynx Inc HS 54 User Manual Page 2 Revision 2 01 July 2009 1 Document Overview The purpose of this document is to explain the functionality of the HS 54 This will include general information of the Headstage and a chart explaining the mapping of all of the tetrode channels 2 Headstage 54 Overview This manual should be used with the Neuralynx EIB board EIB 54 Kopf manual The Neuralynx Headstage Amplifier is the active electronic part of the Hyperdrive Electrode Interface Board Headstage Tether system It provides 54 channels of Unity Gain amplification buffering and locations for up to 28 surface mount light emitting diodes for use in experiments which involve animal position tracking It also provides connections for the electrode inputs to the EIB and output connectors for the dual cable 72 conductor tether The Headstage Amplifier is 56 millimeters in diameter Two 37 pin micro D connectors are used for tether connections Four connectors on the bottom of the board are used to connect the Headstage Amp
4. the use of the Shorting Plug or proper usage of Static Protection including Static Wrist Straps please contact Neuralynx at 406 585 4542 or email Support Neuralynx com Neuralynx Inc HS 54 User Manual Page 9 Revision 2 01 July 2009 10 HS 54 EIB 54 Tether Combination Channel Mapping Table 1 HS 54 Channel Mapping Tetrode on the EIB Board Tether labeled on HS AD Channel AD Channel AD Channel AD Channel m TEE EE E A E E EA m2 T3 T4 Ooo m o f i 2 3 TTS 16 oo o oom A a y s ee 7 i T7 Ooo o mm eae 9s w n TT 179 T10 I a eae T11 T12 ae Se ee ee eee Figure 2 Via Labeling on EIB Table 2 HS 54 Reference Mapping Reference Channels on EIB Tether labeled on HS References Labeled in DRS References Labeled on ERP 27 El E2 E3 E4 R1 R2 NOTE the above mapping examples are hardware system dependant The channels are mapped from the HS to Tether to Tether Adapter The type of hardware you are using will designate the correct mapping in the Cheetah Acquisition Software For this example we will be using a Digital Lynx system with DRS boards For this specific example let s use a 4 board system 64 channels which will include two Input Boards and two DRS Boards IDID The board to the extreme left will be in position 0 and the board to the far right will be board 3 The headstage will have two tethers Neuralynx Inc HS 54 User Manual Page 10 Revision 2 01 July 2009 c
5. i Neuralynx High Density Electrophysiology Recording Systems HS 54 User Manual Revision 2 01 July 2009 Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 Fax 406 585 9034 www Neuralynx com support Neuralynx com Table of Contents I Document OveryieW asada rill eel eie aeia ein ieii res naa e Reisa 3 2 Headst ge DA OVerVi eW oscri eserita aanita atea a laer e oean S 3 De NOS SAN e E a cha cy eee AEREE E E ule Ba e a Es Bde 4 4 Tether Signal Omne Crrcin ei 5 cach25 ac ccaedssmesoseiencads souzach te suacsucte iactetienacatasoaechtececctuataeeiee 5 5 Power Supply Requirements secs rescesducsclte fevas assasvanedey aussaadtacggate devas tavesacgaansausbesagucuetdedaes 6 6 Mounting the Headstage to the EIB ow eee eesecssneeceeeeeceeececseeeeceseeecseeeeeseeeeesaes 6 7 Tetrode Adjustment Tool Alignment Cutouts 2000 0 ee eesecsseceseeeeeeeeseecsaecneesseeeenees 7 8 LED s Power Usage and Customization esssseessesessssseeesstsrtsesstsetsesseseeseseeeessesee 7 9 Practical TipSisusiesinn nenn nn an a a A aa n ea ES 8 9 1 Antistatic Precautions ninun n a a a es feet cect a 8 9 2 Maintenance and Cleaning Information ssssssessessseseseeessseessresseesseeesseessseessese 8 9 3 Shorting Plug Board description and use sssesseesseessensseesseessetesstessesseesseesseee 8 10 HS 54 EIB 54 Tether Combination Channel Mapping eecceeeseeceeeeeeneeeees 10 List
6. l be about 15 milliamps LED power is separate from amplifier power LED power requirements and operation is described below NOTE Care must be taken when powering the Headstage Amplifier Both 5v and 5v supplies must be applied and removed at the same time Each tether connector contains conductors for the 5v and 5v amplifier power This redundancy will result in better reliability and less voltage drop on the power conductors in the tether 6 Mounting the Headstage to the EIB The EIB is permanently attached to the Hyperdrive The Headstage Amplifier is mounted to the EIB by placing the Headstage Amplifier on top of the EIB and carefully tightening the hold down screw in the center of the Headstage Amplifier The alignment block provides proper connector pin alignment between the two boards In practical daily use the Alignment Block allows convenient and proper connection even if the animal is moving This system also allows the Headstage to be removed without damage bending to the double male signal connection pins used between the Headstage Amplifier and the Tetrode Interface Board The bottom side of the Headstage Amplifier contains 4 female connectors arranged in a rectangular pattern mirroring the connector pattern on the topside of the EIB Each board has female socket type connectors to reduce the possibility of damage to the board assemblies An additional connector set double male headers are used to make the connec
7. l deionized water solution Shake excess rinse solution off The board must be dried before using and may be placed in a dry incubator at 40 degrees C This is the process used for final cleaning after assembly 9 3 Shorting Plug Board description and use Each Headstage is shipped with an EIB which has been modified to connect short each of the Headstage Electrode Input signal connections to Ground This modified EIB is referred to as a Shorting Plug This Shorting Plug provides additional static protection Neuralynx Inc HS 54 User Manual Page 8 Revision 2 01 July 2009 during shipping It should also be used between recording sessions or whenever the headstage recording system will not be used for an extended period of time Please note that the Headstage opamps contain electronics industry standard static protection circuitry which protects against damage when applied to the standard human body discharge model therefore it is safe under normal handling conditions during animal hyperdrive connection We recommend placing the shorting plug as shipped in between recording sessions as an additional static protection measure Also note that Neuralynx highly recommends the use of a static wrist strap whenever handling ANY exposed electronics or when handling ANY implanted animal because cell damage will occur in the event of even slight static discharge to implanted electrode connections If you have any questions regarding
8. lifier to the Neuralynx Tetrode Interface Board Low noise low power low input bias current Opamp have been used on this device instead of the traditional Source Follower FET circuit used in the traditional headstage amplifier design in order to obtain exact unity gain which greatly improves Common Mode Rejection Ratio CMRR performance for the entire recording system for artifact and other common mode noise signal rejection lower output impedance to reduce noise susceptibility of the tether and other signal cabling provide integral antistatic protection on each input channel insure low input bias current levels and eliminate signal distortion NOTE Because an Opamp is used for the unity gain buffer amplifiers special care and attention must be given to the power supply design and power application and removal sequencing The input protection circuitry will lower the input impedance if the input voltage exceeds either power supply voltage This can occur by either raising the input voltage signal above the power supply voltage or by the loss of either power supply voltage In practical use this is not a problem since the tether connectors make a robust connection and redundant wires are used in the tether for the power supply connections The 2 power supplies should be applied simultaneously with a double pole DPDT switch The Neuralynx EEG Reference Panel contains power supplies which properly sequence power supply voltage and monito
9. mall amount of heat glue or other removable adhesive can be used to hold the double male header connectors to the bottom side of the Headstage Amplifier This will help the header connectors stay with the Headstage Amplifier when the animal is disconnected Remember that you will want to replace the header connectors if one is damaged or bent 9 1 Antistatic Precautions As with all electronics static discharges cause damage to semiconductor devices and especially to impedance inputs The Opamp inputs are protected against a 2000 volt discharge but care must still be used when handling and using the Headstage Amplifier Please observe the following guidelines e always wear a grounding wrist strap when handling an animal which is connected for recording e always wear a grounding wrist strap when handling the Headstage Amplifier and e Store the headstage in the black antistatic protective box in which it was shipped Static discharge damage will usually result in lower amplifier input impedance and noisier amplifier channel performance 9 2 Maintenance and Cleaning Information The Headstage Amplifier is not coated with any covering and is therefore susceptible to contamination from dirt animal food fingerprints and wastes If recording signals become noisy check for contamination on the topside AND the bottom side of the board The board may be cleaned with hot soapy water Ivory dish soap is the best followed by a rinse with a 50 alcoho
10. oming from the board These tethers are labeled P1 and P2 on the silkscreen of the HS Tether P1 will be inserted into the DRS board 1 and the tether P2 will be inserted into the DRS board 3 Now from the chart above we read that TT1 uses AD channels 8 11 We also know that the DRS board in slot 1 uses up 32 place AD channels So from the example above the P2 tether will start at AD 64 IB 1 will use AD channels 0 31 IB 2 will use AD channels 64 95 in the Digital Lynx because it is attached into the DRS in the 3 slot With this information we can then deduce that TT1 will be occupying AD channels 72 75 Neuralynx Inc HS 54 User Manual Page 11 Revision 2 01 July 2009
11. r headstage currents Neuralynx Inc HS 54 User Manual Page 3 Revision 2 01 July 2009 3 Glossary EIB An Electrode Interface Board EIB is the interface between experiment electrodes in a microdrive and the appropriate headstage IB Neuralynx Input Board AC DC DRS Digital Reference Selector Board Neuralynx Inc HS 54 User Manual Page 4 Revision 2 01 July 2009 4 Tether Signal Connections Two 37 pin micro D type connectors are used for the headstage tether connections Tether cables consist of a pair of shielded 36 conductor cables to give a total tether connection of 72 wires with overall shields Signal type assignments input output and power for the two connectors are identical meaning that the same pins on each connector are used for the same type of signal For example pin 37 is used for the shield pin 21 is a reference electrode output This allows the connectors to be reversed without damage to the Headstage or animal however proper connections must be made or recording channels will be reversed The individual wires in the Tether are 44 gage stranded conductors with a resistive measurement of approximately 3 ohms per meter The Shield pin 37 has a resistance measurement of about 1 ohm total for a 5 meter cable Connector towards the front
12. s current limiting resistor on the bottom side of the board The two LEDs and the resistor are connected in series There are also 4 small via holes between the LED mounting positions These vias provide connections to LED positive power voltage LED negative power voltage The LED end of the series connected LED LED Resistor circuit and The resistor end of the LED LED Resistor string This allows for a very flexible LED configuration If you need to have a custom LED configuration please contact Neuralynx for advice on the LED mounting and wiring configuration If you do not need the LEDs you may leave Neuralynx Inc HS 54 User Manual Page 7 Revision 2 01 July 2009 them unpowered off A good practical use is to power the LEDs along with the amplifier power to indicate power application to the amplifier circuitry 9 Practical Tips ALWAYS WEAR AN ANTISTATIC WRIST STRAP WHEN HANDLING AN ANIMAL ORWHEN HANDLING THE HEADSTAGE AMPLIFIER These are available at most electronics stores It is very convenient to leave the headstage amplifier physically connected to the in between experiment recording sessions When this is done protect the headstage by placing it in the black antistatic shipping box A piece of black conductive foam in the bottom of the box will also help Keep the double male header connectors attached to bottom side of the Headstage Amplifier and not to the top of the Tetrode Interface Board attached to the animal A s
13. tions between the two boards allowing for easy replacement of a damaged header connector and proper vertical spacing between the two boards Note The header pins should normally be left with the Headstage Amplifier and not with the Electrode Interface Board As stated above there are 4 connectors between the Headstage Amplifier and EIB The front connector is a single row 14 pin connector the rear connector is a single row 16 pin connector and the left and right connectors are a double row 18 pin connector with the pins arranged in a 2 x 9 pattern Note that the 2 dual row left and right connectors have an offset the left side connector is one pin position to the rear of connector on the right side Care must be taken to insure that the Headstage Amplifier is attached with the proper front to rear orientation Headstage power should be turned off when connecting and disconnecting to the animal A spare set of the 4 double male header connectors is included with the Headstage Amplifier Extra sets of the header connectors may be ordered from Neuralynx Neuralynx Inc HS 54 User Manual Page 6 Revision 2 01 July 2009 7 Tetrode Adjustment Tool Alignment Cutouts There are 14 semi circular recesses on the outside edge of the Headstage Amplifier printed circuit board These recesses or cutouts accommodate the Tetrode Adjustment Tool and help the user align the tool during insertion and adjustment Note that each tetrode number TT1 through TT12
Download Pdf Manuals
Related Search