マニュアル Monitor UV
Contents
1. 002 9Y VIVSddN YL Ad NJAIMS NI GALNIYd Yj GE imagination at work2. Protected ground 1 Remote Control Adaptor Code No 19 6008 01 is available as an accessory to provide screw in socket connection The functions autozero and event mark can be controlled from a controller like the Gradient Programmer GP 250 or GP 250 Plus or the Liquid Chromatography Controller LCC 500 LCC 500 Plus or LCC 500 CI Connect a Remote Control Adaptor Code No 19 6008 01 to the socket Input Output Connect one or two Signal cables Code No 19 6006 01 between the Remote Control Adaptor and the controller PORT SET 8 1 to autozero and PORT SET 8 0 approximately 0 1 min later to make the port ready for use again Autozero Pin 14 Output 21 Autozero Pin 15 Output 23 Ground Event mark Pin 6 Output 22 Event mark PORT SET 9 1 to make an Pin 15 Output 23 Ground event mark and PORT SET 9 0 approximately 0 1 min later to make the port ready for use again 3 Installation With the controllers LCC 500 LCC 500 Plus and LCC 500 CI you also connect the UV M II to the monitor input socket MONITOR 1 or MONITOR 2 Use the signal cable Code No 19 6006 01 supplied or the Y cable Code No 18 0577 01 and choose the 100 mV output voltage to minimize electrical noise Connect the positive wire into the 100 mV terminal and the negative wire into the 0 terminal Connect the ground wire to either the controller or the monitor ground terminal 17 4 Operation 4 1 4 2 4 3 18 Routine start up
3. 2 General Description 2 1 2 General Description Principle of The UV M II Optical Unit houses the lamp Hg or Zn the operation wavelength filter and the flow cell The light beam is directed through a conical or straight flow through cuvette 6 pl or 2 pl illuminated volume to a photo detector The photo detector current is passed to the signal processing circuitry in the Control Unit Fig 1 Optical Unit Inlet Lens Beam splitter Flow cell Photodetector Lam E Outlet sossoereoss Recorder output Fig 1 The operation principle of the Monitor UV M II The reference signal comes from the same point in the lamp as the signal measuring the sample thus assuring a stable baseline by negating the effects of variations in lamp intensity The Hg lamp emits light only at some certain wavelengths it does not emit light at 280 nm so for this wavelength the light is converted at a fluorescent surface before it passes the filter On the lamp housing there is special exit for the 280 nm light which necessitates a change in lamp position when working at this wavelength 2 General Description 2 2 Control Unit Front panel s e LZ Amersham e amp Biosciences 5 RANGE AU 2 F amp FY FS H eS 3 Ss Ss N oe amp e sogo o S nN amp TIME CONST 4 Fig 2 Control Unit Front panel No Item Description 1 lamp on off 1
4. Choice of wavelength Lamp power 4 Operation This is a short instruction on how to start up the UV M II You will find more detailed information in Section 4 2 to 4 9 1 Make sure that the installation procedure is properly carried out see Section 3 The Lamp on off LED should be lit If not see Section 4 3 2 Select a wavelength by rotating the filter wheel on the Optical Unit and position the moveable lamp housing on Q for 280 nm or for all other wavelengths 3 Allow 1 hour warm up after a cold start The UV M II is warmed up when the base line is fully stable 4 Depress auto zero mark key until the key LED illuminates If the LED flashes see Section 6 Zero the recorder Select the desired sensitivity range by pressing one of the range AU keys 7 Verify the Time constant by pressing both range AU key at the same time TIME CONST CHECK The LED corresponding to the set Time constant will flash Changes can be done on the rear of the Control Unit see Section 4 7 1 Open the protective black plastic filter wheel cover on the Optical Unit see fig 4 2 Rotate the filter wheel to the desired position A click will indicate that the filters is in position 3 Position the lamp housing Hg with the mark on the lamp housing facing the same symbol as in seen on the wavelength label i e when working at 280 nm and when working at other wavelengths 4 Close the filter wheel cover Note Itis ver
5. Switches lamp on off Key with indicator lamp 2 Indicates the lamp power function 3 Indicates if lamp is connected 1 2 autozero mark Autozero when depressed at least Key with indicator lamp 2s 2 Base line adjustment when used together with the range AU keys 3 Event mark 10 deflection 3 range AU TIME CONST 1 Select range values 0 001 2 AUFS CHECK keys a range AU to select from 2 to 0 001 AUFS or b range AU to select from 0 001 to 2 2 Verify the Time constant seconds by pressing both keys which causes the LED above the set value to flash 4 TIME CONST The LED above the set value flashes when pressing both range AU keys 5 range AU LED showing sensitivity range 2 General Description Rear panel Input Output z a Optical 100 10 0 Unit Time constant mV Before instailation and service see below Fig 3 Control Unit Rear panel No Item 1 Mains inlet with voltage selector and fuse holder 2 Lamp 3 Input Output 4 Time constant Signal output 6 Optical Unit Underside Description Socket for mains cable Selects mains voltage 100 120 220 230 or 240 V Mains fuses 1 x 400 mA for 100 120 V 60 Hz 1 x 200 mA for 220 230 240 V 50 Hz Inlet for the lamp cable Warning Disconnect the lamp cable only with the lamp turned off A 15 pin D SUB connector for external control of event mark and autozero and for signal output Sets time constant to 0 1 1 0 or 10s The output signal is a
6. sssssssssssssssessseessresssrsssesseeeseresse 14 3 9 Connection of the Optical Unit to the Control Unit 15 3 10 Connection to a recorder se ssssssssssssssssessresseressresssressesssee 15 3 11 Remote connection i aeit araa aea ria eneoti iape AKETE 16 3 12 Connection to a controller ss nsssseossosssssseesssseeessssees000 16 A r Operatora E Ee EE EEEE EAN Tiea O aa Aaaa ELTE iae 18 4d Ro utine start Upsir eerti aater eeeausnseucdetibeaes dee 18 4 2 Choice of wavelength ssnsesssnssssssesosssseeessssesssosesssseeess 18 43s ALAM Pi pOWEE o eoe atei oaae aie OEE E T esi 18 AA WALID iis ereinda a io dr aa 18 AD AMICOZEEO riae a AE A E E EEEE NEn 19 4 6 Selection of sensitivity range eessesssssssssesseesseresssesssess 19 4 7 Selection of Time constant dswansesecseapurisdaareweveseeies 19 A B Pent Mark cencorenennn n a aa eons 19 4 9 Base line adjustment s esssssssesssessssseccssssecssssessssssessseeses 19 X MAINTENANCE dereat e e e EER 20 5 1 Instrument nousing 2 ccdecstesdentersedd acestriersterrde axcvuneuns shorts 20 5 2 Cleaning the flow cebl vss czceszcntes csceene asGasee titans 20 5 3 Gleaming the filters ssc ccvsecavceseecas saree ccepercedendeadececsvevecteuvees 21 5 4 Changing a defective lamp sssssssssssessseessresssssssesseeeseresse 21 GA Trouble shooting sieners an es a s e 23 7 echnical Spe ificationSunsnnssneaiinna tiari ieies ii 25 Control Unit serimi a n E aiet 25 Optical Unites
7. local safety regulation when disposing of mercury waste 21 5 Maintenance a Unscrewing the lamp Fig 13 Changing a defective lamp b Removal of the lamp 1 Remove the two screws on the lamp housing end plate which is attached to the power cable Fig 13 2 Carefully slide the lamp out of the lamp housing Insert the new lamp into the lamp housing and secure the end plate with the two screws Do not touch the lamp 22 6 Trouble shooting 6 Trouble shooting The Monitor UV M II has been designed for trouble free use If good chromatographic practice is followed very little difficulty should be experienced Clean optical surfaces are essential if low noise levels are to be maintained The following check list is meant to be a guide in trouble shooting If the checks in this section are executed and the UV M II does not work properly consult your local GE Healthcare representative Symptom Cause Remedy Panel keys do not work lamp on off LED does not light lamp on off LED flashing lamp on off LED on no recorder Autozero LED flashing Lamp plug connected or disconnected with mains power on 1 Mains cable not connected 2 No voltage at wall socket 3 Fuse blown 1 Lamp cable not connected 2 UV lamp power malfunction 1 Control Unit not connected to recorder 2 Recorder not operating Recorder zero not set correctly 4 Recorder range incorrect Wrong filte
8. procedure When the monitor is not in use clean the cell and use the protective red plugs to cover any open holes or keep the flow cell connected Store it dry or filled with distilled water Never allow any solution to dry out in the cells Never try to dry a cell with compressed air as such air contains microscopic oil particles If a cell has to be dried use clean nitrogen Liquids flowing through the cells should be free of suspended particles and degassed to prevent the formation of bubbles Always 13 3 Installation 3 8 Connection to 14 the column make sure that the cell is clean dirt in the cell may be detected by viewing the light path with a magnifying glass See Section 5 1 for instructions to clean the cell If using preflanged tubing for FPLC or Standard Chromatography drop the Tubing connector FPLC Fig 11 a into the inlet of the flow cell and secure it in position by fingertightening a preflanged tubing over it Repeat this procedure for the outlet of the flow cell Fig 11 a Tubing gt connector FPLC with gt corresponding tubing 1 Fix the Optical Unit directly under the column on the Scaffold holder Note Always position the Optical Unit with the filter wheel cover facing upwards Prepare the outlet tubing from the column with the appropriate Tubing connector if unflanged Figs 11 b and c gt o N 2 Fig 11 b Tubing connector standard with correspo
9. 10 or 100 mV DC signal The monitor is connected to a recorder via the 3 pole connector supplied 100 or 10 mV to plus O to minus Inlet for the Optical Unit signal cable Precautionary instructions are fixed to the underside of the Monitor UV M II Control Unit Read them before using or servicing your UV M II 2 General Description 2 3 Optical Unit Filter housing 4 Lamp cable 3 1 Lamp housing end plate 2 baie eaten VY Filter wheel cover 5 Optical Unit detector housing cable 9 Protective cover 8 Detector housing 6 Flow cell aperture 7 Fig 4 Optical Unit No Item Description 1 Lamp housing Consists of a black heat distributor the lamp and the lamp cable Two lamps Zn and Hg are available the Zn lamp housing is the larger The Hg lamp housing slides to two positions relative to the filter housing d for detection at 280 nm e for the other wavelengths Lamp housing end plate Loosen to exchange lamp Lamp cable Connects to the Control Unit Filter housing Contains the filters Four screws fix the filter housing to the detector housing 5 Filter wheel cover A black plastic cover that gives access to the filter wheel with the filters The filter wheel rotates with a click to three positions each placing a different filter in the light path 6 Detector housing Contains the flow cell and the photo detector 7 Flow cell Fits into the top of the detector housing being f
10. arefully draw chromic acid into the cell Do not draw acid into the syringe 4 Allow the acid to remain in the cell for 10 20 minutes Eject the cleaning solution carefully without splashing and rinse the cell with a distilled water 100 ml b ethanol distilled water 50 v v 20 ml c distilled water 20 ml 6 Replace the cell in the Optical Unit and reconnect the system to be monitored For optimal performance it is essential that the interference filters are clean and free of any particulate material Do not touch the interference filters Should the filters become contaminated with dust finger prints or oil proceed as follows 1 Carefully take out the filter from the housing without touching or scratching the filter surface see Fig 8c 2 Use lens cleaning tissue dipped in ethanol to gently clean the filter surfaces 3 Place the clean filter back into the filter wheel for installation or into its protective bag for storage The mercury lamp has an expected lifetime of approx 8 000 hours the reflective surface used for 280 nm approx 4 000 hours and the zinc lamp approx 2 000 hours Warning Before changing a defective lamp ensure that the lamp cable is disconnected from the Control Unit to prevent injury to eyes If the mercury lamp is broken make sure that all mercury is removed Warning If the mercury lamp is accidently broken carefully remove all mercury and glass to prevent mercury poisoning Follow
11. e No No per pack Lamps and filters Hg optics with filters 18 0604 02 1 Zn optics with filter 18 0605 02 1 Hg lamp assembly 18 0630 01 1 Zn lamp assembiy 18 0631 01 1 Filter Wheel 18 0647 01 1 Filter 214 nm 18 0622 01 1 Filter 254 nm 18 0620 01 1 Filter 280 nm 18 0621 01 1 Filter 313 nm 18 0623 01 1 Filter 365 nm 18 0624 01 1 Filter 405 nm 18 0625 01 1 Filter 436 nm 18 0626 01 1 Filter 546 nm 18 0627 01 1 The optics with filter s contain 1 Lamp 1 Lamp housing 1 Filter wheel lor 2 filters Zn 214 nm Hg 254 and 280nm 1 filter strip with label s all assembled The lamp assemblies contain lamp and lamp housing Flow cells Filow Cell 2 mm 18 0684 01 1 Flow Cell 5 mm 18 0675 01 1 The flow cells contain 1 Flow Cell 1 Assembly Kit 2 Tubing conneclor standard 2 Tubing conneclor FPLC 1 116 Ferrule PTFE Liquid connections Tubing connector standard 18 0765 01 2 Tubing connector FPLC 18 0766 01 2 Protective cover 18 0763 01 10 Assembly kit 18 0768 01 1 Assembly kit containing 1 cone removel tool 2 protective cover 2 M6 lubing nipple stainess steel Other Accessories and Spare Parts Remote controi adaptor 19 6008 01 1 Fuse holder 5x20 220V 19 8654 01 1 Fuse holder 6 3x32 110V 18 0847 01 1 Mains Cable 220V 19 2448 01 1 Mains Cable 110V 19 2447 01 1 Scaffold Holder 18 0716 01 1 Aluminium support rod 18 0552 01 1 Fiow Restrictor 18 1012 07 1 Keyboard and Overlay 18 1003 96 1 27 28
12. ead volume Cell shape Cell material in direct contact with liquid Flow range Pressure Tubing connection type Dimensions Weight Sensitivity specifications Static short term noise Dynamic short term noise Static long term noise Dynamic long term noise Static drift Dynamic drift Flow sensitivity Test conditions Dynamic condition Response time Room temperature Wavelength Flow cell 254 313 365 405 436 and 546 nm Lifetime 8 000 h 280 nm Lifetime 4 O00 h Wavelength 214 nm Lifetime 2 000 h Interference Circular 1 27 cm diameter Wavelengths 214 254 280 313 365 405 436 and 546 nm Electronics designed according to IEC and UL Flow cell 2 mm Flow cell 5 mm 2 0 0 2 mm 5 0 0 1 mm 2 ul 6 ul 30 ul 10 ul Horizontal double cone with inlet and outlet distributor Straight flow through cuvette Quartz Quartz Suprasil Suprasil ETFE titanium ETFE O 1 000 ml h O 1 000 ml h Max 40 bar Max 40 bar Flanged FPLC Unflanged type standard Capillary 1 16 o d metal HPLC 100 x 100 x 50 mm Wx Lx H 0 4 kg 3x 10 AU 6 x 10 AU 2 x 10 AU 4x 10 AU 1 x 10 AU h 2 x 104 AU h 2 x 104 AU min ml 1 ml min methanol ls 25 0 2 C 254 nm 5 mm cell 8 Accessories and Spare Parts 8 Accessories and Spare Parts Please order accessories and spare parts according to the designations and code numbers given below Designation Cod
13. irmly held into place by the locking nut on the underside 8 Protective cover A protective cover around the flow cell inlet outlet protects the Optical Unit from liquid coming in contact with the electronics 9 Signal cable Connects to the Control Unit 3 Installation 3 1 Site requirements 3 2 Unpacking 3 3 Choice of horizontal or vertical position 3 Installation Some important notes regarding installation e Be careful to avoid spillage on the Optical Unit Always use the protective cover to prolong monitor lifetime see Section 3 7 e Always make sure that the locking nut is turned to the stop to avoid base line drift see Section 3 7 e Always use the correct Tubing connector to avoid leakage see Section 3 8 e Always position the Optical Unit with the filter wheel cover up wards see Section 3 8 e Connect the column outlet tubing to the top on the 5 mm cell and to the bottom on the 2 mm cell see Section 3 8 The UV M II is designed for use under normal laboratory conditions The atmosphere should be free of both excess humidity and corrosive or contaminated vapours which may form deposits on the optical surfaces To minimize drift the temperature should be kept constant The Optical Unit should be placed away from draught heat and direct sunlight or any other influence which may cause large temperature variations The UV M II may be operated at ambient temperatures in the range 4 40 C The
14. m 0 001 to 2 AUFS and then jump to 0 001 to repeat the sequence 4 range AU will cycle range from 2 to 0 001 AUFS and then jump to 2 to repeat the sequence Select an alternative range by depressing one of the range AU keys The Time constant in the Monitor UV M II is factory set at 1 second Choose the Time constant according to the peak widths in the chromatogram The shorter Time constant the faster response in the monitor both for peaks and noise Choose a higher Time constant for broader peaks than for narrower peaks Technique Recommended Time constant Standard Chromatography 10orl1ls AALS lorO 1s HPLC lorO 1s To select another setting take the following steps 1 Insert a thin screwdriver into the Time Constant hole on the rear of the UV M II see Fig 3 and turn the arrow to the appropriate setting of 0 1 1 or 10s 2 Verify the Time constant by depressing both range AU keys at the same time The LED indicator for the corresponding TIME CONST will flash Depression of the autozero mark key less than 1 second activates an event mark A spike of 10 full scale will mark the spot on your chromatogram Event mark can also be performed from a remote control See Section 3 1 and 3 12 Depress autozero mark and one of the range AU keys at the same time lt range AU will raise the baseline level p range AU will lower it The new base line is maintained until a new sensitivity range is selected or a new autozero procedure i
15. nding tubing Fig 11 c 1 16 Ferrule PTFE the Tubing connector for HPLC with 4 corresponding tubing 3 Screw the column outlet tubing directly into the top of the Optical Unit for 5 mm flow cell or the bottom for 2 mm flow cell Fig 12 Both connections provide all upward flow direction in the cell i e minimizing the risk of trapping air in the flow cell Screw to fingertightness Check for leakage when starting to run and if necessary tighten the tubing further with the supplied wrench 3 Installation 3 9 Connection of the Optical Unit to the Control Unit 3 10 Connection to a recorder Fig 12 Connection of the column to the two flow cells i T 4 Connect the Optical Unit outlet tubing into the opposite hole Use the cone removal tool in the Assembly kit when removing the Tubing connector FPLC Screw the tool a few mm into the hole in the Tubing connector and remove the cone The lamp cable connects into the Lamp socket on the rear of the Control Unit see Fig 3 To disconnect the cable press the outer security clasps and remove the plug The Optical Unit signal cable plugs into the Optical Unit socket on the rear of the Control Unit see Fig 3 To disconnect the signal cable lift the lower security clasp and pull the plug out Warning When the lamp is on the lamp socket carries a dangerous voltage Do not connect disconnect with the lamp LED on Tool
16. nine Sexceksetaas ond eE EEE ATE aE E 26 8 Accessories and Spare Parts sssesssessesesssesssrssssesseessreeseresseesssess 27 1 Introduction 1 Introduction The GE H ealthcare Monitor UV M II Control Unit Code No 18 1001 10 Hg Optics with 254 and 280 nm filters Code No 18 0604 02 or Zn Optics with 214 nm filter Code No 18 0605 02 is a fixed wavelength monitor for liquid chromatography The UV M II consists of a control module and a small optical unit which makes it easy to place it optimally in the system State of the art electronics and high precision optics offer flexibility and accuracy in UV M II detection The UV M II is ideal for all types of liquid chromatography primarily FPLC and HPLC The Optical Unit has interchangeable mercury and zinc lamps an analytical high precision double cone 5 mm path length or a preparative straight 2 mm path length flow cell The double cone shape of the analytical cell minimizes the collisions between light beams and cell wall and maximizes the illuminated volume i e it gives an increased sensitivity Easy selection of different filters means there is good choice for diverse application needs This unit may be connected directly to any chromatography column thus minimizing the dead volume between the column and the monitor The Control Unit has touch panel design and solid state electronics Functions such as autozero and event mark can be executed from a remote source
17. o the filter position i e the label should be placed opposite to the filter Fig 8 e 11 3 Installation 3 6 Change of the 12 lamp assembly Fig 8 e Filter wheel accessories 9 Reassemble the circular plastic band with the filter wheel peg fitting into the band notch Fig 8 e 10 Check that all filters are clean Place the filter wheel back into the filter housing It can be placed only in the correct position 11 Reassemble the filter housing to the detector housing by fastening the four screws Tools needed Philips screwdriver gt Fig 9 Changing the lamp type For changing a defective lamp see Section 5 3 Warning Before changing a defective lamp ensure that the lamp cable is disconnected from the Control Unit to prevent injury to eyes If the mercury lamp is broken make sure that all mercury is removed 1 Detach the end plate by removing one and loosen one of the two holding screws on the lamp housing on the Optical Unit Slide the lamp housing off the filter housing 3 Detach the end plate as in step 1 above from the lamp housing you are going to attach to the Optical Unit 4 Slide the lamp housing onto the filter housing guides with the light aperture facing the filter housing The lamp and signal cables should be on the same side As you slide the lamp housing into position depress the two pressure pads on the filter housing in sequence to facili
18. on it is respectfully suggested that authors wishing to use these designations refer to their trade mark status at least once in each article Copyright 1995 GE Healthcare Bio Sciences AB All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means without permission in written form from the company Contents Ti Tite CCH OI icseetcasayssdeueseceaes vasuieaceheead e AE SEE E EE ease 3 2 General DEsCri pron cxtcne es elses shoiaowouarenestevterac e 4 2 1 Principle of operation esssssssssessesesssesssessseeseressressersssesssee 4 22 Control Unit nirera eor n A r E E E ie roata 5 Front paneleran aene eae aaa oO SA EE EaR REEN 5 Rear paneler renis r aanas aoao a aa e SEa 6 Under SIG 1 5 6 innein i a s 6 2 3 Op cal Unitsanseece n E E a taey 7 De Installation eesin E E E T E es epee 8 Some important notes regarding installation 0000 8 3 1 Site TEQUIFEMENTS cnici reie i E e a 8 3 2 Unpacking eere areas o E e iE Er a 8 3 3 Choice of horizontal or vertical position s00000000050000 8 34 Mains installation ssssesessisaioes iret inieako Motendda deo inee eet 9 Goon Bilton Stall ation ccssears clases vudesaauacuredens dues dcedargeeat resis 10 3 6 Change of the lamp assembly s ngcsonccvdesteinestoextsteeseeisets 12 3 7 Flow cell installation ssssesseeesssesssessseessresssrssssesseessresse 13 3 8 Connection to the column
19. power consumption of the monitor is 25 VA Note It is important that the filters flow cells and lamps are not handled during unpacking For protection of these items they should remain in their packing materials until required for use For a complete monitor you need one Control Unit one Hg optics or one Zn optics with filter s and one flow cell Carefully unpack the Monitor UV M II Check the contents against the packing list supplied Inspect for any damage that may have occurred during transit Report any damage immediately to the local GE Healthcare representative and to the transport company concerned Save the packing material for possible future transport The Monitor UV M II can be used horizontally or vertically Carefully or attach the correct front panel 1 Remove the protective film from the keyboard on the front of the instrument 2 Remove the protective film from the back of the front panel 3 Position the front panel onto the front of the Control Unit see Fig 2 Ensure that the touch panel keyboard on the front panel fits onto the instrument keyboard 3 Installation 3 4 Mains installation 4 Attach the rubber feet accordingly If the UV M II is to be used in FPLC System choose the horizontal front panel and place the feet so that the monitor fits on top of the P 500 pumps Tools needed Screwdriver Before connecting this instrument to the mains supply for the first time please read these ins
20. propriate solvent followed by thorough rinsing with clean solvent Laboratory detergents of the type used to decontaminate glassware may also be used Oily deposits which increase the tendency to trap air bubbles can be removed by rinsing the flow cell first with a non polar solvent e g hexane then with a polar solvent e g isopropanol and finally with distilled water or with detergent Persistant contaminants may be removed by either of the following procedures depending on the nature of the contaminant Warming the cleaning media up to 40 C may improve the result Cleaning with detergent 1 Remove the flow cell from the Optical Unit 2 Fill the cell with a 10 solution of RBS 25 Deconex or equivalent and let it stand for at least two hours Rinse the cell with a distilled water 20 ml b ethanol distilled water 50 v v 20 ml c distilled water 20 ml 4 Replace the cell in the Optical Unit and reconnect the system to be monitored 5 Maintenance 5 3 Cleaning the filters 5 4 Changing a defective lamp Cleaning with chromic acid Warning Chromic acid is extremely corrosive Be careful at handling and treat spills immediately with a large excess of water 1 Prepare fresh chromic acid by adding concentrated sulphuric acid 100 ml to a saturated solution of sodium dichromate 3 5 ml 2 Remove the flow cell from the Optical Unit Connect a glass syringe to the outlet of the cell and c
21. r 6 Wrong lamp Faulty electronics 1 Optical Unit not connected to Control Unit 2 Flow cell not installed w Wrong lamp position Wrong filter Locking nut not properly closed Dirty cell Aging lamp Dirty filter oN E Disconnect the mains cable for a short while Plug in Check by plugging table lamp in Replace fuse if fuse blows again immediately consult GE Healthcare representative Connect lamp cable Consult service engineer Connect Control Unit make sure that the response green connector snaps in Check recorder function Zero recorder Set recorder range to UV M II output range 10 or 100 mV Change to the correct filter Check which lamp is installed Contact service engineer Connect Optical Unit Install flow cell Check that the lamp position and the wavelength fit together Change to the correct filter Turn the locking nut to stop Clean the cell Check lamp Replace if necessary Clean the filter 23 6 Trouble shooting Symptom Cause Remedy Excessive noise short term 1 Poor ground contact Check contact to ground 2 Filter wheel cover not properly Close the cover closed 3 Locking nut not properly closed Turn the locking nut to stop 4 Recorder range incorrect Set recorder range to UV M II output range 110 or 100 mV 5 Recorder connections incorrect Check connection between output terminals and recorder make sure that the green connector snaps in properly 6 Dir
22. s needed Small screwdriver 1 Connect the supplied signal cable to the 4 pole connector provided with the Control Unit by loosening the connection screws and inserting the cable wires for the correct output voltage Connect the positive wire into the 10 mV or 100 mV terminal choose the 100 mV if possible to minimize electrical noise the negative wire into the 0 terminal and the ground wire into the terminal 2 Tighten the connection screws and ensure that the wires are properly connected by pulling them gently 3 Connect the plug into the Control Unit socket It should snap in 4 If using the REC 111 REC 112 the supplied signal cable is connected to the recorder with the Pin connectors banana type supplied with the recorder 15 3 Installation 3 11 Remote connection 3 12 Connection to a Controller 16 Connect the Communication cable Code No 19 6005 02 to the remote socket marked Input Output on the rear panel The following functions and signals are available INPUTS TTL compatible Autozero low Zeroes the base line 14 Event mark low Gives a 10 full scale 6 spike on monitor signal OUTPUTS 1 V AU Monitor signal not filtered 11 Vr Voltage on reference 9 diode not filtered Vs Voltage on sample diode 2 not filtered Autozero ready low Gives a signal during 7 autozero Analog ground To be used with signal 10 outputs Digital ground To be used with autozero 15 and event mark signals
23. s performed 19 5 Maintenance 5 1 5 2 Cleaning the 20 Instrument flow cell 5 Maintenance Wipe the instrument regularly with a damp cloth Let the instrument housing dry completely before use A clean flow cell is essential for the proper operation of the UV M IL Ensure that the flow cell is not allowed to dry out if it contains a liquid with dissolved salts proteins or other solutes with low volatility Do not allow particles to enter the flow cell The cells may be inspected for particles and air bubbles by removing the flow cell and examining the light path through the window with a magnifying glass Note It is possible to remove the flow cell without disconnecting the top tubing Unscrew the flow cell locking nut and slide the Optical Unit housing down from the flow cell If the cell contains trapped particles proceed as follows 1 Remove the flow cell from the Optical Unit 2 Connect a syringe to the outlet tubing and flush a clean solution of ethanol in distilled water 50 v v through the cell in small aliquots Examine the cell from time to time to see that the particles have been washed out 3 Rinse the cell with particle free distilled water about 20 ml and replace it in the Optical Unit 4 Replace the cell in the Optical Unit and reconnect the system to be monitored Most non particulate contaminants e g denatured proteins salts etc can be removed by flushing the cell with the ap
24. st see Section 3 6 2 Remove the four screws in the filter housing Separate the filter housing from the detector housing Fig 8 a Fig 8 a Removing the filter housing 3 Carefully remove the filter wheel from the filter housing Fig 8b 3 Installation Fig 8 b Filter wheel in filter housing 4 Fig 8 c Removal of filter from wheel J Fig 8 d Placement of filter in wheel If necessary remove filter s from the filter wheel by pressing it them out e g with a small screwdriver Fig 8 c Filters are sensitive optical components Never touch thee optical surfaces or exposure them to temperatures above 60 C Clean them with dry lens cleaning tissue and store them when not in use in the container in which they were supplied Heavy contamination may be removed by using a lens tissue dipped in ethanol Place the filter s of choice into the filter wheel max 3 filters with the correct orientation with the mirror side facing upwards and position over one of the three triangular apertures The filters snap in by pressing them quite firmly Do not touch the filter surface Fig 8 d y Trang ar aperture f 7 Remove the circular plastic band showing the wavelength s Remove labels from the band if necessary Place the correct labels in the hand with label designation facing outwards Ensure that the label position corresponds t
25. tate the installation 5 Replace the lamp housing end plate 6 Slide the lamp housing firmly into place There will be a faint click when the housing is positioned correctly The Hg lamp housing can take two positions while the Zn lamp housing has only one position 3 Installation 3 7 Flow cell installation There is one analytical 5 mm and one preparative 2 mm flow cell available Both cells are installed in the same way as below 1 Remove the red protective plugs from the detector housing and the flow cell Fig 10 a Protective cover for flow ce 2mm Tien Fig 10 a Flow cell gt O ring types protective eine Eh stone cover and protective spat plug Protective plug for detector aperture 2 Place the flow cell into the detector housing from above it is impossible to position the flow cell incorrectly Fig 10 b Flow cel Detector housing Locking nut Fig 10 b Installation of the flow cell in the detector housing 3 Secure the flow cell by turning the locking nut until the stop Fig 10 b If the locking nut is not tightened sufficiently the monitor will function poorly e g drifting base line 4 Place the protective cover around the flow cell see Fig 4 to protect the electronics inside the Optical Unit from liquid spillage Avoid spillage as much as possible for prolonged monitor lifetime 5 To remove the flow cell reverse the
26. tructions 1 The Control Unit is supplied with mains cables and fuse kits for both 100 120 V and 220 240 V operation Choose the 400 mA fuse kit for 100 120 V operation and the 200 mA for 220 240 V Discard the unwanted fuse kit and mains cable immediately pA Remove the yellow warning label covering the fuse voltage selector unit on the rear panel 3 Open the fuse voltage selector cover with a thin screwdriver Fig 5 Fig 5 Opening the fuse voltage selector cover Insert the screw driver at the top centre and apply pressure according to the figure 4 Install the correct fuse holder and fuse into the right hand position Fig 6 Fig 6 Installlng the fuse holder Always use the hole at the righthand side when you install the fuse holder 5 Remove the rotary voltage switch and replace it with the correct voltage showing Fig 7 3 Installation 3 5 10 Filter installation Fig 7 Choose the correct voltage by turning the voltage selector 6 Close the cover The correct voltage should be visible through the window 7 Connect the instrument to a grounded mains outlet Tools needed Screwdriver Philips screwdriver The Hg optics with 254 and 280 nm filters and the Zn optics with 214 nm filter are delivered with the filters installed If other filters are to be used install new filters 1 Ifthe Zn lamp is attached remove the lamp housing fir
27. ty cell Clean the cell 3 Dirty filter Clean the filter 4 Wrong filter Change to the correct filter 5 Wrong lamp position Check that the lamp position and the wavelength fit together 24 7 Technical Specification EMC standards Safety standards 7 Technical Specification Control Unit Full scale ranges AU Autozero Base line adjust Event mark Electronic filter Remote Input Output Recorder output Linearity Output impedance Environment Humidity Power requirements Power consumption Dimensions Weight 0 001 0 002 0 005 0 01 0 02 0 05 0 1 0 2 0 5 1 2 Range 0 2 to 2 to AU Reproducibility 1 full scale Set time 3 seconds Control front switch or remote Adjustable up to 100 full scale Will give a 100 of full scale spike Control activated by front switch or remote Response time selectable 0 1 1 or 10 s 10 to 90 of full scale 2nd degree Bessel filter for no peak distortion Note Traditionally time constant refers to the time taken for the output signal to rise from O to 63 Thus a response time here of 1 0 second corresponds to a traditional time constant of 0 5 seconds and so on Connector type 15 pin D SUB connector female Description Pin 1 V AU Output not filtered 11 Vr Output not filtered 9 Vs Output not filtered 2 Analog ground 10 Autozero base line TTL input 14 Event mark TTL input 6 Autozero ready TTL output 7 Digital gro
28. ty cell Clean the cell Deposits on filter Clean the filter 8 Solvent with high UV absorbance Change to a more suitable solvent or another wavelength where the solvent does not absorb 9 Impure chemicals Filter the liquids before use Change to purer chemicals 10 Bubbles passing through the cell Degas solvent Check for leaks Connect a Flow Restrictor after the Optical Unit 11 Aging lamp Check lamp Replace if necessary 12 Excess noise on mains supply Use alternative power source or remove source of disturbance Excessive baseline drift 1 Variable absorbance gradient Change to another solvent or another wavelength without absorbance Contaminated solvent Use fresh solvent Use purer grade 3 Large temperature variation Relocate Optical Unit or remove source of temperature change 4 Instrument not warm Allow 1 hour warm up Bubble trapped in the cell Clean the cell Connect a Flow Restrictor 6 Locking nut not properly closed Turn the locking nut to stop Long term noise often regular 1 Temperature variation especially in Relocate the Optical Unit or remove waves in recorder response cold room source of temperature change 2 Flow rate variations Check pump system and column packing 3 Poor ground contact Check contact to ground 4 Dirty cell Clean the cell 5 Deposits on filter Clean the filter 6 Locking nut not properly closed Turn the locking nut to stop Low sensitivity 1 Aging lamp Check lamp Replace if necessary 2 Dir
29. und 15 Protected ground Vr Voltage on reference diode Vs Voltage on sample diode active low 100 mV full scale over range to 5 V 50x 10 mV full scale over range to 0 5 V 50x O common c safety ground Connector type screw terminal 1 up to 2 AU at 254 nm 5 up to 2 AU at other wavelengths 200 ohm 4 to 40 C 10 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure 10 95 100 120 220 230 240 V 10 50 60 Hz 25 VA 245 x 304 x 92 mm Wx Lx H 28 kg This product meets the requirement of the EMC Directive 89 336 EEC through the harmonized standards EN 50081 2 emission and EN 50082 1 immunity Note This is a class A product In a domestic environment this product may cause radio interference in which cases the user may be required to take adequate actions Note The declaration of conformity is valid for the instrument when it is e used in laboratory locations e used in the same state as it was delivered from GE Healthcare except for alteration described In the User Manual e used as stand alone unit or connected to other CE labelled GE Healthcare products or other products as recommended This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the harmonized standard EN 61010 1 25 7 Technical Specification 26 Optical Unit Hg lamp Zn lamp Filters Safety standard requirements Optical path length Illuminated volume Total d
30. user Monitor UV M II um 56 1035 84 Edition AF GE imagination at work Important user information Reading this entire manual is recommended for full understanding of the use of this product The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument Should you have any comments on this manual we will be pleased to receive them at GE H ealthcare Bio Sciences AB S 751 82 Uppsala Sweden GE Healthcare Bio Sciences AB reserves the right to make changes in the specifications without prior notice Warranty and Liability GE Healthcare Bio Sciences AB guarantees that the product delivered has been thoroughly tested to ensure that it meets its published specifications The warranty included in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by GE H ealthcare Bio Sciences AB GE Healthcare Bio Sciences AB shall in no event be liable for incidental or consequential damages including without limitation lost profits loss of income loss of business opportunities loss of use and other related exposures however caused arising from the faulty and incorrect use of the product Trade marks FPLC are the exclusive trade marks of GE Healthcare Bio Sciences AB In view of the risk of trade mark degenerati
31. y important that the cover is properly closed otherwise you will get stray light into the monitor After mains power connection lamp power activation is indicated by a continuous glow the lamp on off LED A flushing light indicates that the lamp power cable is not connected Turn the monitor off connect the cable to the Lamp socket see Fig 3 and depress the lamp on off key to turn the Lamp on To turn the lamp off depress the lamp on off key for 2 seconds until the indicator goes off This safety feature prevents the lamp from being accidentally switched off 4 Operation 4 4 Warm up 4 5 Autozero 4 6 Selection of sensitivity range 4 7 Selection of Time constant 4 8 Event mark 4 9 Base line adjustment From a cold start the UV M II is fully operational after one hour warm up for the system For stable operation at very high sensitivity a longer warm up time is recommended Use the autozero if the recorder goes out of range 1 Depress the autozero mark key for 2 seconds until the autozero LED indicator goes on The Control Unit now performs an autozero procedure to create a reference zero base line This new base line will be maintained and is independent of selected range or Time constant If the LED flashes refer to Section 6 2 Zero the recorder with recorder zero control Autozero can also be performed from a remote control see Sections 3 11 and 3 12 p gt range AU will cycle range selection fro
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