Miscellaneous, Product brochure - Bio
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1. r pf 8 BY y P LA MR O j n e ne t Z C O n nN eS C A GI N A supporting life sciences Miscellaneous Product Brochure I nterest in any of the products request or order them at Bio Connect Bio Connect B V T NL 31 0 26 3264450 TBE 32 0 2 503 03 48 Begonialaan 3a F NL 31 0 26 326 44 51 F BE 32 0 2 503 03 27 z 6851 TE Huissen E info bio connect nl The Netherlands W www bio connect nl m Miscellaneous Transfection Cell Culture Luciferase Assay Extraction Reagents DNA Synthesis Equioment fod Bo Culture Cells Transfection Reagent Avantgene Transfection Reagent AvantGeneTWM2 is a broad range transfection reagent that efficiently delivers DNA molecules into a variety of cultured cells AvantGene has been extensively tested by Allele scientists in Hela 293 293T CHO COS 7 HUVEC 3T3 L1 NIH 313 MCF 7 Vero E6 C2C12 and rat L6 muscle cells and found to be superior to many other transfection reagents cDNA expressing plasmids Allele s RNAi LineSilenceTM cassettes and Allele s RNAi SilenCircleTM plasmids have been tested with AvantGene reagent T O 0 A 5 O T Usage Tips AvantGene reagent has low cell toxicity and can work in a wide range of concentra tions For a 24 well plate transfection use 2 5 ul AvantGene reagent as a starting point In general more AvantGene reagent within a 2 3 fold range from the starting point2. SUS ae lt 12 min from 20 to 100 C 12 min from room temp to room temp 10 C gt 15 min from 100 C to room temp 10 C A 1 5 mL x 40 B 0 5 mL x 54 C 0 2 mL x 96 D 15mm diameter x 24 E 2 0 mL x 40 29 5 x 26 5 x 1 0em 8 5 kg 110VAC 50 60 Hz 125W Quantity 1 unit www allelebiotech com 7 16 Equipment Miscellaneous Incubators ThermoCube500 ThermoCube500 Cooling amp Heating Blocks provide temperature control and a convenient means to incubate samples This unit employs Peltier effect heat exchange technology to provide accurate stable temperature control It can be used witha selection of interchangeable blocks for plates and tubes Purchase blocks separately Features e Programmable for up to 5 sequential steps of different temperature and duration e Large LCD display and simple controls make an easy to use interface for programming e Displays set temperature actual temperature running time and time remaining e Automatic stabilizing PID control to maintain set temperature e Wide Temperature Setting Range e Sample blocks are easily removed for cleaning or replacement SPECIFICATIONS Sample Blocks Heating Time Cooling Time Model ThermoCube500 Temperature Setting Range 10 to 105 C Temperature Control Range 0 to 100 C Timing Range 1 min to 99 hrs 59 or continuous Display Accuracy lt 0 5 C Temperature Control Accuracy JOSE Block Temperature Uniform
3. be used with a select ion of interchangeable block for plates and tubes Features Digital controled Peltier heating cooling Exact temperature control with PID circuit Gentle reliable orbital mixing up to 1500 rpm Special damping provides quiet operation even at high speeds Choose from several standard sample blocks for plates or tubes Custom blocks are available to satisfy special experimental requirements Large VFD display and simple controls provide a friendly user interface Conforms to CE safety standard Sample Blocks Block A Block B 1 5 ml x 40 0 2 ml x 96 Block C BlockH 0 5 ml x 54 11 53 7 3 3 8cm Product Name Ampicon ThermoMix Shaker Models Temperature Setting Range Temperature Control Range Timing Range for mixing Mixing Speed Mixing Amplitude Temperature Control Accuracy Display Accuracy _ Temperature Uniformity Heating Time Cooling Time Standard Blocks Dimensions LxWxH Net Weight Power Supply MB 101 The ability to hold low temperatures with the stated accuracy varies with room temperature so in a room at 20 C the lower end of the range would be 6 C 20 C minus 14 C Catalog ThermoMix500 Introducing Cost Efficiency to Research Thermoshakers 7 15 SPECIFICATIONS T O r D 5 O T ThermoMix500 0 to 105 C Room Temperature 14 to 100 C 1 Minute to 99 Hours 59 Minutes 300 to 1500 RPM 3mm lt 00 C
4. experiments dye terminator sequencing reactions cloning and other techniques Phosphoramidite chemistry is generally used for the solid phase synthesis of the oligo nucleotides which is carried out by sequentially adding the desired nucleotide units via its 3 terminal hydroxy group to an immobilized oligonucleotide chain We offer all four DNA nucleosides deoxyadenosine deoxycytidine deoxyguanosine and deoxythymidine covalently attached to a CPG particle for your synthesis needs T O 0 D 5 O T Features Use This product is used to put dN at the 3 end of oligonucleotides CPG size 1000 A Formula N A Purity 95 Diluent N A Stability in Solution N A Highly effective and gentle method for protein extraction from mammalian Fully dialyzable and may be used cells eliminates cell scraping from conjunctively with subsequent assay culture dish systems Product Name Catalog Quantity 3 dA CPG 1000 A AHP CH DACPG01 per gram 3 dC CPG 1000 A AHP CH DCCPG01 per gram 3 dG CPG 1000 A AHP CH DGCPG01 per gram 3 dT CPG 1000 A AHP CH DTCPGO1 per gram Introducing Cost Efficiency to Research www allelebiotech com Modification CPG DNA Synthesis 3 3 Amino Modifier C7 CPG Q introduces a primary amine to functionalize the 3 terminus of the target oligonucleotide amp can be use to attach a variety of modifiers i e activated NHS ester or isothio rT cyanate fluoresce
5. results in higher transfection efficiency however higher amount of reagent might also result in toxicity in certain cells Transfection of GFP plasmid into rat For more tips visit www allelebiotech com muscle cells using AvantGene Starting Conditions for Using Avantgene Culture Plate 12 well 24 well 96 well 0 32 cm2 5 20 ul 2 5 10 ul 1 25 5 ul 0 5 1 25 ul media Product Name Catalog Quantity AvantGene Transfection Reagent ABP TC ATR125U 125 uL ABP TC ATR250U 250 uL ABP TC ATR 50U 750 uL Introducing Cost Efficiency to Research www allelebiotech com Miscellaneous 7 2 Cell Culture insect Cells Sf21 Insect Cells The Sf21 cell line was cloned from pupal ovarian tissue of the Fall Armyworm Spodoptera frugiperda by Vaughn et al The Sf21 cell line is highly susceptible to infection with Autographa California nuclear polyhedrosis virus ACNPV baculovirus and can be used with all baculovirus expression vectors Because Sf21 cells have a large size they provide higher expression levels of some proteins Product Name Catalog Quantity Sf21 Insect Cells Frozen ABP CEL 10007 1x 10 7 cells Sf9 Insect Cells The Sf9 cell line was derived from pupal ovarian tissue of the Fall armyworm Spodoptera frugiperda The Sf9 cell line is highly susceptible to infection with Autographa california nuclear polyhedrosis virus AcNPV baculovirus and can be used with all baculovirus expression vectors Sf9 cells are com
6. x H A7X34X26cm programming simple and the finished instruct Net Weight SS Hok ion set is easily uploaded to the Thermal Cycler Interface IRS232 Product Name Catalog Quantity Ampicon PCR Thermocycler ThermoExp500 ThermoExp500 1 unit www allelebiotech com Introducing Cost Efficiency to Research
7. 5 O T Miscellaneous 7 6 Protein Analysis Extraction Reagent AlleleExtract B Protein Reagent AlleleExtract B Protein Reagent provides a single step method of gently extracting proteins from a wide range of bacterial cells Using this reagent eliminates the need for repeated sonication and freezing thawing steps that are commonly used to release recombinant proteins from E coli Extracted proteins are suitable for many affinity purification and analysis methods e g GST and 6xHis purification systems If preferred DNAse I lysozyme protease inhibitor salts etc may be added to the reagent AlleleExtract B Protein Reagent offers several fold increase in the yield of soluble proteins when compared with other commercial lysis systems Features Nonionic detergent in either PBS or Tris based buffer Eliminates mechanical disruption and provides a gentle and efficient method for extracting proteins Achieves high yield of soluble proteins Compatible with most purification systems May be used for soluble and insoluble protein extraction from bacterial cell lysates Product Name Catalog Quantity AlleleExtract B Protein Reagent PBS Buffered ABP PA AXBR500P 500 mL ABP PA AXBR125P 125 mL AlleleExtract B Protein Reagent Tris Buffered ABP PA AXBR500T 500 mL ABP PA AXBR125T 125 mL www allelebiotech com Introducing Cost Efficiency to Research Protein Analysis Extraction Reagent AlleleExtract M
8. MED 10001 1L AlleleBalanced Luciferase Assay Kit AlleleBalanced Luciferase Assay Kit is a novel formulation for measuring fire fly Photinus pyralis luciferase gene luc expressions in mammalian tissue culture cells or in bacteria Resulting from Allele Biotech s extensive research and development efforts this brand new assay system offers extremely sensitive detection tools for those using luc as reporter It provides a simple reaction system balancing sensitivity and stability to suit most routine Luciferase based gene expression analysis The kit can be used in low or high throughput applications at a much reduced cost The AlleleBalanced Luciferase Assay Kit is very easy to ship store and use Features Applications High sensitivity gives more signal than Suitable for molecular and cellular studies compared leading brands Pe Enhancer promoter analysis with luc Stability offers maintenance of more than as a reporter gene 95 signal level after 3 hours l Enzymatic screening assays Easy to use simply lyse the cells mix and read Control experiments using Luciferase for transgene expression RNAi Low cost shipping the reagents are stable for a few days at room temperature eliminating the need for cold packaging Product Name Catalog Quantity AlleleBalanced Luciferase Assay Kit ABP PA ABLA001 100 RXNS ABP PA ABLA011 1000 RXNS Introducing Cost Efficiency to Research www allelebiotech com T O O D
9. Protein Reagent AlleleExtract M Mammalian Protein Extraction Reagent provides an effective and gentle method for extracting proteins from mammalian cells This reagent uses a non ionic detergent in either PBS products with catalogue number ending with P or 25 mM Tris pH 7 4 and 250 mM products with catalogue number ending with T to extract nuclear and cytoplasmic protein from cultured cell lines AlleleExtract M may be used conjunctively with reporter assays immunoassays and protein purification It is easily digested with protein assays such as Western blotting Coomassie Blue Bradford and BCA Protein Assays T O O D 5 O T Features Applications Highly effective and gentle method for Extracting proteins from mammalian cells protein extraction from mammalian cells for most molecular biology applications eliminates cell scraping from culture dish including Western Blotting Coomassie Blue Bradford BCA Protein Assays and Fully dialyzable and may be used others conjunctively with subsequent assay systems Product Name Catalog Quantity AlleleExtract M Protein Reagent PBS Buffered ABP PA AXMR500P 500 mL ABP PA AXMR125P 125 mL AlleleExtract M Protein Reagent Tris Buffered ABP PA AXMR500T 500 mL ABP PA AXMR125T 125 mL New AlleleExtract I Protein Reagent Product Name Catalog Quantity AlleleExtract Protein Reagent PBS Buffered ABP PA AXIRS500P 500 mL ABP PA AXIR125P 125 mL AlleleE
10. e Serum ABP MED FBS0050 50 mL ABP MED FBS0500 500 mL Grace s Insect Culture Medium Grace s Insect Medium was developed in 1962 and has been shown with the addition of serum to support the growth of insect cells including Spodoptera frugiperda Allele s Grace s Insect Culture Medium Unsupplemented is serum free and ideal for transfection because it does not contain lactalbumin hydrolysate or yeastolate which can interfere with transfection When supplemented with L glutamine lactalbumin hydrolysate and yeastolate Grace s Insect Medium can be used for general growth and maintenance of insect cells Product Name Catalog Quantity Grace s Insect Medium Unsupplemented ABP MED 10004 1L Introducing Cost Efficiency to Research www allelebiotech com 1 4 Miscellaneous Cell Culture Serum and Media Serum Free Insect Culture Medium Allele s Serum Free Insect Culture Medium is specifically developed for the Baculovirus Expression Vector System BEVS technology It supports excellent growth and propagation of Sf9 Sf21 T ni High Five and other insect cells and yields high levels of virus replication and recombinant protein production The Serum Free Insect Culture Medium has been tested to produce high cell yields and superior recombinant protein expression results with multiple cell lines and in a variety of culture environments The Serum Free Insect Culture Medium is optimized for high density cell gr
11. e varying requirements of PCR applications Interchangeable blocks allow the ThermoExp500 to adapt to plates tube strips and tubes 0 2 0 5 ml Including an Unique dual sample block permits execution of two different programs simultaneously and independently L 3 O Q Q T te Features e Very fast ramping rate saves 20 of your PCR time e Large LCD display for easy monitoring of process e Eight interchangeable blocks for different sample presentations e Independently controlled dual blocks makes one machine equal two e The gradient block allows to easily find optimal PCR condition Specifications Temperature Range 4 99 C Heating Rate max 4 C second Cooling Rate max 3 C lsecona Block Temperature Uniformity k04C Block Temperature Accuracy EWES ma Hot Lid Temperature Range oc noe Gradient Range Block G only From 1 30 C Temperature Control Mode Block Tube Program Storage 99files j Maximum Segments 5 segments Maximum Program Steps 16 steps Maximum Cycles 99 cycles Large LCD display provides easy monitoring Power Supply A710V 50Hz 200W of process Windows based PC software makes Size L x W
12. h www allelebiotech com 7 14 Equipment MS100 Dry Bath Shaker Miscellaneous Specifications eTemp range 0 MX to 100 X TSC 96 or 5X above ambient temp to 100 X TS 96 eTemp accuracy within 0 5 X Display precision 0 1 X eHeating rate from 20 X to 100 X X20 min eTimer range 1 min 99 h 59 min Shaking speed 200 rpm 1500 rpm eMixing orbit 2mm Power input 100 120 VAC 50 60Hz 150W Dimensions 354 x 240 x 170 mm eNet Weight 6 kg Product Name Allelequip Thermoshaker 50C 990C Thermoshaker Features eCombines a shaker and dry bath incubator in one Back lit LCD display of temperature and time eSimultaneous display of set point and actual value eAdjustable parameters of temperature shaker speed and time to meet wide range of applications eProgrammable two segments of parameter settings eAudio alert upon program completion Eight models interchangeable sample blocks to accommodate 0 2 ml to 50 ml tubes The unit has been individually tested for its function and carries one year warranty CE certified Catalog Quantity MS 100 1 unit Introducing Cost Efficiency to Research www allelebiotech com Equipment ThermoMix500 ThermoMix500 combines the functions of mixing and temperature control providing a convenient means to incubate samples This unit employs Peltier effect heat exchange tec nology to provide accurate stable temperature control It can
13. he provided 12 VDC power supply the aluminum sample blocks is automatically maintained at between 0 2 C It is designed for keeping sample tubes at ice temperature in many experi mental procedures It is a simple and convenient replacement of wet ice bucket Two models are currently available Contents HE One thermoelectric chilling device model H203 96 or H203 48 One 12 V power supply adaptor One sample block cover One block extractor One User Manual Aluminum sample blocks for H203 96 Four 0 2 ml sample blocks Two 1 5 ml sample block or for H203 48 Two 0 2 ml sample blocks One 1 5 ml sample block Specifications Operation ambient temperature 8 30 C Sample block steady state temperature 3 Beene 2imi sample blocks H203 96 thermoelectric Peltier dry bath chiller installed and block cover applied Place the chiller on your lab bench or a similar Electrical input 12 VDC 4A H203 96 or 3A sturdy flat surface The space between the bottom H203 48 of the unit and bench surface should be clear Dimensions 185 x185x90 mm 7 25 7 25x3 5 There should be at least two inches 5 cm of in clearance around the unit in order to allow sufficient ventilation Product Name Catalog Quantity H203 Ice Temp Incubator 48 OoC 40C H203 48 1 unit H203 Ice Temp Incubator 96 OoC 40C H203 96 1 unit www allelebiotech com Introducing Cost Efficiency to Research Equipment Chilling Hea
14. ity lt 0 5 C lt 35 min from 20 to 100 C 25 min 20 to 0 C www allelebiotech com Blocks A 1 5mL x 40 Block A Block B size x positions By Ae baa 1 5 ml x 40 0 2 ml x 96 D 15mm diameter x 24 E 2 0 mL x 40 Water Bath LxWxH 11 5 x 7 3 x 3 8 cm Heating Parts TE Module Cooling Parts TE Module Size 30 x 20 x 15cm Weight 3 3 kg Power Supply 220VAC 50Hz 120W Available for 110VAC Notes Block C BlockH 1 The performance parameters above are based on 1 5 mL X 40 standard block TE Peltier 0 5 ml x 54 ISX 3X heat exchange 3 8cm 2 The base price does not include a sample block Please order the desired block s from the listing at Block profile Catalog ThermoCube500 1 unit Product Name Quantity Ampicon Digital Control Dry Bath ThermoCube500 Introducing Cost Efficiency to Research a Equipment Incubators 7 17 ThermoMini500 ThermoMini500 feature the same quality and reliability as the ThermoCube Dry Bath series but in a smaller light weight fixed block format ThermoMini500 models offer digitally controlled heating for a variety of sample formats ThermoMini500 models are powered by a 12 VDC power adapter that wil operate on 110 or 220 VAC T O 0 D 5 O 7 Features e Lightweight and compact easily moved from place to place e Aluminum alloy sample blocks are easy to clean and disinfect e Digital time and temperature controls and displays e Peltier design pro
15. monly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins Product Name Catalog Quantity Sf9 Insect Cells Culture ABP CEL 10002 1x 10 7 cells Sf9 Insect Cells Frozen ABP CEL 10006 1x 10 7 cells T ni Insect Cells The T ni Insect Cell Line derived from ovarian cells of cabbage looper Trichoplusia ni have been shown to be capable of expressing significantly higher levels of secreted recombinant proteins compared to other insect cells In addition it also offers faster doubling time when compared to Sf9 or Sf21 Orbigen s T ni Insect Cells have been adapted for growth in serum free medium Product Name Catalog Quantity T ni Insect Cells Culture ABP BVD 10005 1x 10 7 cells T ni Insect Cells Frozen ABP CEL 10008 1x 10 7 cells www allelebiotech com Introducing Cost Efficiency to Research Cell Culture Serum and Media Fetal Bovine Serum USDA Approved A Economical Alternative to US Sourced FBS 4 Origin Mexico o Complete traceability and extensive document trail o Complete control of processing o Mexico is considered free of BSE FMD amp SVD by the USDA re Performance tested on common cell lines o BHK 21 A o COS 7 o Hela o L 929 o Sf 9 Mycoplasma and Endotoxin tested Sterile 0 1 um triple filtered Supplied in gamma irradiated sterile PETG bottles For in vitro research only Product Name Catalog Quantity Allele Validated Fetal Bovin
16. nt label to an oligo oO can be used to attach an oligo to a solid surface a users may print their own oligonucleotide based microarrays Product Name Catalog Quantity 3 Amino Modifier C7 CPG AHP CH AMI0103 100mg AHP CH AMI1003 1000mg 3 Biotin CPG can be used to detect proteins using various methods i e non radioactive immunoassays cytochemical staining cell separate isolation of nucleic acids detection of specific DNA RNA sequences by hybridization probing of conform ational changes in ion channels provides means for purification Product Name Catalog Quantity 3 Biotin CPG AHP CH BIO0103 100mg AHP CH BIO1003 1000mg 3 Dabcyl CPG a commonly used dark quencher due of its light absorbance properties and lack of residual fluorescence dabcyl s absorption properties limit the range of fluorophores it can quench to those emitting at 400 550 nm with max absorption at 471 nm doubly labeled oligonucleotides can be used as diagnostic probes for DNA and RNA quantification and to discriminate single nucleotide variations Product Name Catalog Quantity 3 Dabcyl CPG AHP CH DAB0103 100mg AHP CH DAB1003 1000mg www allelebiotech com Introducing Cost Efficiency to Research _ DNA Synthesis Modification CPG 7 11 3 FAM CPG label oligos with fluorescein a fluorophore with an isosbestic point equal absorp ri tion for all pH values at 460 nm at the 3 end D doubly labeled oligon
17. owth and contains a high density lipid shear protectants and optimum levels of glucose and L glutamine Supplementation is generally not required Formulation X Contains 50 ug ml gentamycin X Optimum growth temperature is 27 1 C Product Specifications X Optimum pH is 6 1 6 3 Test Method pH O Poemtometie 6 64 X Most insect cell lines can be transferred Appearance Visual inspection Clear yellow solution directly into the Serum Free Insect Culture Endotoxin LALgelcot 10 00EUml Medium without adaptation Seeding Sterility Current USP No growth densities should be 4 x 105 1 x 106 membrane filtration eee omer atnculrure Osmolality Vapor point elevation 350 380 mOsm kg X The Serum Free Insect Culture Medium has been tested in stationary culture stir flasks shaker flasks and Fermbach flasks for scale up to bioreactors Product Name Catalog Quantity Serum Free Insect Culture Medium ABP MED 10002 1L www allelebiotech com Introducing Cost Efficiency to Research Cell Culture Serum and Media TNM FH Insect Culture Medium TNM FH medium is fully supplemented Grace s medium including trace medals lactalbumin hydrolysate yeastolate and 10 heat inactivated fetal bovine serum This medium is ideally suited for growth infection and protein expression of insect cell lines This medium may be used for suspension or monolayer cultures Product Name Catalog Quantity TNM FH Insect Culture Medium ABP
18. ting Dry Bath 7 13 H203 Chilling Heating Dry Bath Incubator H203 dry bath incubators use thermoelectric device Peltier effect device to heat and chill samples H203 75C 100C let you set the temperature between 0 C 75 C 100 C It is useful for temporary storage of cells samples and enzymes for enzymatic reaction and heat inactivation and for cell transformation T O 0 D 5 O T Contents One thermoelectric chilling device model H203 75C 100C One 12 V power supply adaptor One sample block cover One block extractor One User Manual Aluminum sample blocks for H203 96 Four 24x0 2 mL blocks and two 6x2 mL Twelve 1 5 mL tube adaptors to use 1 5 mL microtubes in 2 mL blocks Specifications Power supply 12 VDC 5A Temperature range 0 C to 75 C H203 75C H203 100C Chilling Heating Dry Bath Incubat 0 C to 100 C H203 100C PS rer cae eee Temperature precision 1 C i H203 75C 100C has a modular design of sample Sample block temperature evenness 0 5 C blocks The user can select and combine various Dimensions 185 x 185 x 90 mm block modules to accommodate different sample Weight 1 5 kg tubes or plate for example 0 2 0 5 1 5 or 2 mL Package weight including sample blocks 2 5 kg tubes or a whole 96 well plate or 96 well flat bottom culture plate Product Name Catalog Quantity H203 Ice Temp Incubator 75C H203 75C 1 unit Introducing Cost Efficiency to Researc
19. ucleotides can be used as diagnostic probes for DNA and RNA quantification and to discriminate single nucleotide variations singly labeled oligonucleotides can be used for DNA sequencing and in situ hybridization re 7 Product Name Catalog Quantity 3 FAM CPG AHP CH FAM0103 100mg AHP CH FAM1003 1000mg 3 Thiol Modifier C6 S S CPG important in the production of diagnostic probes terminal thiol group allows attachment of an oligo to various ligands i e biotin alkaline phosphatase or fluorescent dyes allows for the attachment of an oligo to a solid surface via a maleimide linkage Product Name Catalog Quantity 3 Thiol Modifier C6 S S CPG AHP CH TC60100 100mg AHP CH TC61000 1000mg Oligo Modification Phosphoramidite 5 FAM can label the 5 end of an oligo with a fluorescein a fluorophore with an isosbestic point equal absorption for all pH values at 460 nm fluorescein has an absorption maximum at 494 nm and emission maximum of 521 nm doubly labeled oligonucleotides can be used as diagnostic probes for DNA and RNA quantification and to discriminate single nucleotide variations Product Name Catalog Quantity AHP CH DAB0103 100mg 3 Dabcyl CPG AHP CH DAB1003 1000mg Introducing Cost Efficiency to Research www allelebiotech com Miscellaneous 7 42 Equipment H2030C Dry Bath H203 Ice Temp Incubators H203 is a thermoelectric Peltier dry bath chiller Connected to t
20. vides thermal efficiency reliability e Choose from five standard blocks o Available block sizes36 x 0 2 mL tubes 15 x 0 5 mL tube and 8 x 1 5 mL tubes The 96 x 0 2 mL block accepts 0 2 mL tube strips and 96 wells plates Sam ple Blocks SPECIFICATIONS Models ThermoMix500 Temperature Setting Range 0 to 105 C Temperature Control Range Room Temperature 14 to 100 C Timing Range for mixing 1 Minute to 99 Hours 59 Minutes Mixing Speed 300 to 1500 RPM Mixing Amplitude 3mm Temperature Control Accuracy lt 00 C Display Accuracy lt 05C ____ Temperature Uniformity OC Block A Block B Heating Time lt 12 min from 20 to 100 C 1 5 ml x 40 0 2 ml x 96 Cooling Time 12 min from room temp to room temp 10 C gt 15 min from 100 C to room temp 10 C Standard Blocks A 1 5 mL x 40 B 0 5 mL x 54 C 0 2 mL x 96 D 15mm diameter x 24 E 2 0 mL x 40 Dimensions LxWxH 2915 20 5 7 Net Weight 8 5 kg Power Supply MB 101 110VAC 50 60 Hz 125W Block C BlockH The ability to hold low temperatures with the stated accuracy varies with room 0 5 ml x 54 1 hoe 7 3 X temperature so in a room at 20 C the lower end of the range would be 6 C 20 C minus 14 C 6cm Product Name Catalog Quantity Ampicon Digital Control Dry Bath ThermoMini500 ThermoMini500 1 unit Introducing Cost Efficiency to Research www allelebiotech com Equipment Thermocycler ThermoExp500 ThermoExp500 is designed to meet th
21. xtract Protein Reagent Tris Buffered ABP PA AXIRSOOT 500 mL ABP PA AXIR125T 125 mL Introducing Cost Efficiency to Research www allelebiotech com 7 8 Protein Analysis Insect Lysis Buffer Miscellaneous 5x Insect Lysis Buffer The 5x Lysis Buffer is specifically formulated for insect cells It has been tested in a wide variety of insect cell lines including Sf9 Sf21 T ni High Five and others The high quality freshly added protease inhibitors in the insect lysis buffer ensure the best lysis result and minimal protein degradation Protocol Determine insect cell density cell ml Spin down cells at 2 500 g for 5 min Decant media completely Dilution 5x Insect Lysis Buffer stock into 1x solution using distilled water Put on ice Resuspend cell pellet 4X106 cells in 14ml of ice cold 1X insect lysis buffer Incubate on ice for 45 min Centrifuge at 45K rpm for 30 min Collect clear lysate D AUN SSE Product Name Catalog Quantity 5x Insect Lysis Buffer with Protease Inhibitor ABP BUF 10010 10 mL www allelebiotech com Introducing Cost Efficiency to Research _ DNA Synthesis Standard CPG 7 9 Standard DNA Synthesis CPG Oligonucleotide synthesis is a valuable step in research There are many needs for oligo synthesis sequencing Polymerase Chain Reaction PCR Ligase Chain Reaction LCR reverse transcriptase PCR hybridization studies site directed mutagenesis microarray
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