GD2000 Slab Gel Dryer
Contents
1. manual GD2000 Slab Gel Dryer Vacuum Gel Dryer System um GD2000 IM Rev B0 07 12 Afro e fe r S Contents Important INFORMATION cca ya saavasta vitae Tate ze deg ji Waste Electrical and Electronic Equipment WEEE sssesessireeeeeeerenee n vii 1 Gel Dryer function and description 1 Design Features 2 2 Unpacking the GD2000 isiriirisiatinsasn niagara 3 Setting Up the Vacuum System 21 4 Gel dryer controls ek aaa aa w aaa dorar 5 3 Specificatii ONS iii 7 4 Operating Instruchlons a aaaa aaa eee 8 Step 1 Prepare the dryer eee 8 Step 2 Prepare the gel drying stack 9 Step 3 Set the temperature 12 Step 4 Set the timer eee 13 Step 5 Create a vacuum seg 15 Step 6 Disassembly s nazaaaranssnseasaganaimandaaa mars 15 Options for manual operations 2 16 5 Care and maintenance AA 17 CIEANINS EEN 17 Replacing EE 18 Replacing the rubber sealing sheet 19 6 Troubleshooting onneaan 20 7 Ordering Information 21 Important Information English If this equipment is used in a manner not specified by Hoefer Inc the protection provided by the eguipment may be impaired This instrument is designed for indoor laboratory use only Only accessories and parts approved or supplied by Hoefer Inc may be used for operating maintaining and servicing this product Warning2. Because this instrument can develop suffi cient voltage and current to produce a lethal shock care must be exercised in its operation This instrument is designed in accordance with the EN61010 1 2001 electrical safety standard Never theless it should be used only by properly trained operators Read this entire manual before using the instrument and use only according to the instructions The instrument must always be used with the earth lead of the power cord correctly grounded to earth at the mains outlet Use only undamaged electrical wire and equipment specific for the voltages you will use All equipment connected to high voltage should be in accordance with EN61010 1 2001 Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the instru ment dry completely before use Do not operate the instrument in extreme humidity above 80 Avoid condensation by letting the unit equilibrate to ambient temperature when taking the instrument from a colder to a warmer environment To permit sufficient cooling ensure that the vents of the instrument are not covered Dulezit Informace Czech Pokud by toto za zen je pou ito zp sobem kter neni podle Hoefer ochrana poskytovan na z klad Inc za zen m e b t naru ena Tento n stroj je ur en pro vnit n pou it v laborato i pouze Pouze p slu enstv a sti schv len nebo poskyt nut
3. brengt naar omgevingstemperatuur wanneer nemen het instru ment van een kouder naar een lievere omgeving Om toe te staan voldoende afkoelen verzeker dat de luchtopeningen van het instrument niet bedekt zijn T rke Tietoa Finnish Jos t t varusteita k ytet n tavassa ei m ritetty Hoeferille Inc suojelu ehk isty varusteille saattaa olla avuton T m v line suunnitellaan sis laboratoriok yt lle vain Vain lis varusteet ja osat hyv ksyiv t tai toimitti Hoeferin oheen Inc voi k ytt k ytt miselle valvoalle ja servicing t m tuote Varoittaminen Koska t m v line voi kehitt riitt v j nnitteen ja virran tuottaa kuolettavan j rkytyksen huolta t ytyy harjoittaa toiminnossaan T m v line suunnitellaan EN61010 1 2001 s hk turvallisuusstandardin mukaisesti Silti pit isi k yt t vain ohi oikeasti koulutetut k ytt j t Lue t m kokonainen manuaalinen ennen v linett ja k ytt vain ohjeiden mukaan e V linett t ytyy k ytt aina valtanuoran maalyijyst perusti oikein maadoittaa s hk verkkoaukossa K ytt vain undamaged s hk metallilankaa ja varus teita t sm llinen j nnitteille k ytt Kaikki varusteet yhdistetty korkeaan j nnitteeseen pit isi olla EN61010 1 2001IN mukaisesti Pit v lineen yht kuiva ja puhdas kuin mahdollinen Pyyhi s nn llisesti pehme ll kostealla kankaalla Anna v line kuivua t y
4. ch Hoefer Inc mohou b t pou ity pro provoz dr bu a dr b tohoto v robku Pozor Proto e tento n stroj m e vyvinout dostate n nap t a proud kter m vyr b t a smrtiacej ok p e mus b t vykon v na v jeho provoz Tento n stroj je ur en v souladu s EN61010 1 2001 elektrick bezpe nostn normy P esto e by m ly b t pou ity pouze dn vy kolen oper tor st cel toto ru n p ed pou it m n stroje a pou it pouze v souladu s pokyny P stroj mus b t v dy pou vaj se na v konu zemi v st ra spr vn zemn ny k zemi na s ti v stce Vyu it pouze nepo kozen elektrick dr ty a vybaven pro nap t budete pou vat V echna za zen spojen s vysok m nap t m by m la b t v souladu s EN61010 1 2001 Si ponech n stroje jako such a ist jako mo n Ot ete pravideln s a m kk vlhk m had kem Nech je n stroj nenastaven pln p ed pou it m Nejsou provozov na na n stroj v extr mn vlhkost nad 80 P ede lo kondenzaci o pron jmu jednotky na okoln teplotu nech p i p ij m n n stroj z chladn j do tepl ho prost ed Pro umo n n dostate n chlazen zajistit aby otvory n stroje jsou nevztahuje Vigtig Information Danish Hvis dette udstyr bruges i en made ikke specificeret ved Hoefer Inc den beskyttelse som er blevet forsynet af udstyret kan m ske svaekke
5. d x h Weight Product certifications Indoor use 15 40 C ambient Relative humidity lt 80 for 15 31 decreasing linearly to 50 for 31 40 C Altitude lt 2000 m Installation category II Pollution Degree 2 55 0 x 43 5 x 8 5 cm 21 6 x 17 x 3 4 in 8 kg 17 6 lbs CE UL61010A 1 CSA Important The plate surface of the GD2000 Gel Dryer reaches high temperatures during operation Do not touch the plate surface during operation 4 Operating instructions After you have attached the vacuum pump to the GD2000 follow these instructions to prepare the dryer and gel drying stack Once you set the temperature and the timer the GD2000 automatically starts the vacuum pump and turns on the heat after 10 seconds At the end of a timed run the heat is turned off first and the vacuum ten minutes later Step 1 Prepare the dryer Wipe away all contaminants with a soft damp cloth See Care and maintenance on page 17 for recommendations on how to remove accu mulations of radioactive materials Fit the stain less steel screen into the recess on the platen and then place a sheet of filter paper on the screen slightly larger than the surface area required by the gel s The paper should not extend over the ridge that surrounds the platen Note Cellophane provides a transparent support for densitometric scanning Remove all air pockets at every step of this procedure to avoid scanning distortions Drying
6. open the valve to the gel dryer The extra vacuum should quickly pull the sealing sheet down and accelerate the sealing process Important Once the gel has begun to dry do not break the vacuum seal until the gel is completely dry Gels may crack if you turn off the vacuum before the gel is dry Note Some gels may curl as they dry To minimize curling the vacuum continues for 10 minutes after the heating timer shuts off Note f you manually turn off the vacuum during a continuous run the heat remains on until you also turn it off manually Note If the gel contains radioactive materials and was covered with plastic wrap dispose of the wrap according to local regulations pertaining to radioactive waste Note Fluorescent compounds such as ethidium bromide cannot be visualized after drying Step 5 Create a vacuum seal Ten seconds after you set the timer the vacuum starts automatically if it is connected through the vacuum receptacle on the dryer Watch for a seal to form between the rubber overlay and the platen If the seal does not form almost immediately check stack for misalignment No materials should extend beyond the edge of the recess Assist seal formation by pressing gently at each corner to ensure that the rubber sheet is pulled into the recess Without removing the rubber sealing sheet periodically inspect the gel as it dries When the gel appears dry check the temperature of the gel by brie
7. plo Drying thin or low concentration polyacrylamide gels lt 1 5 mm and agarose gels on paper 0 Lay a sheet of filter paper on top of the filter paper liner and position the gel on this sheet taking care to avoid trapping air beneath the gel e Cover the gel with thin plastic wrap Do not leave wrinkles in the plastic wrap Use the stiff plastic cover sheet with polyacrylamide gels but not with agarose gels Q Check that the edges of all the sheets fall within the recess of the platen If necessary trim the sheets to fit within the recess Cover the stack with the silicone rubber sealing sheet rubber sealing sheet stiff plastic cover sheet n thin plastic wrap gel thickness lt 1 5 mm filter paper filter paper liner stainless steel screen not used with agarose gels Fig 5 Stacks for drying thick gels to filter paper for autoradiography Drying thick gels gt 1 5 mm high concentration gels gradient gels on paper 0 Lay a sheet of filter paper on top of the filter paper liner and then position the gel on this sheet taking care to avoid trapping air beneath the gel e Cover the gel with the porous polyethylene cover sheet with the smooth side toward the gel Check that the edges of all the sheets fall within the recess of the platen If necessary trim the sheets to fit within the recess o Cover the stack wi
8. the gel Fluors become degraded Follow manufacturer s handling instructions paying close attention to recommended temperature exposure o p20 7 Ordering information product guantity code number GD2000 Vacuum Gel Dryer System Includes stainless steel screen VP200 Vacuum pump Vacuum tubing 10 sheets of filter paper 50 sheets of porous cellophane one mylar sheet and one porous polyethylene sheet 115 V GD2001 230 V 1 GD2002 Replacement Parts Filter paper 35 x 44 cm 25 SE1141 Porous cellophane 35 x 44 cm 50 SE1142 Clear silicone rubber sealing sheet 1 SE1143 Stiff plastic cover sheet 1 SE1144 Porous polyethylene cover sheet 1 SE1145 Stainless steel screen 1 SE1146 Vacuum tubing 8 mm i d 3 m 1 VT3 115 V model Detachable power cord 115 V 15 A PSCORD15A 115V Fuses F 12 A 250 V 3AG 5 PSF12A FB 3AG 230 V model Detachable power cord 230 V PSCORD 2230V Fuses T 6 3 A 250 V 5 x 20 mm 5 PSF6 3A SB SX20 e p21 Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support amp hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc Coomassie is a trademark of ICI plc Contrad 70 and Decon 90 are trademarks of Decon Lab O 2012 Hoefer Inc All rights reserved Printed in the USA Hoefer
9. the stainless steel screen and cover sheets and wash separately with a mild laboratory detergent Do not use abrasives or solvents on any part of the dryer Periodically remove accumulations left by autoradiography reagents from the platen and stainless steel screen Apply a strong detergent such as Contrad 70 or Decon 90 for no longer than 5 minutes and rinse thoroughly Dry with a soft towel e pl7 Important Fuses protect eguipment by disconnecting loads too large for the circuit design Always replace fuses with those that conform to the specified fuse rating Important Detach the power cord before replacing fuses Fig 6 Mains power module e p18 Replacing fuses 115 V Model The fuse drawer holds one F 12A 250V 3AG fuse and one shorting coil 230 V Model The fuse drawer holds two F 6 3A 250V 5 x 20 mm fuses The fuse drawer is in the power entry module found on the left side of the control panel See Fig 6 Insert a small flat blade screwdriver into the slot below the fuse drawer See Fig 6 Push in the direction of the arrow to release the drawer Grasp the fuse drawer with your fingers and pull it out e Pull the fuse out of the drawer to inspect it If the fuse element is burned or broken replace it If the fuse appears to be intact check it with a multi meter A reading of 1Q or less indicates the fuse is still usable Push the fuse drawer back in
10. voc usar Todo equipamento conectado a voltagem alta deve ser de acordo com EN61010 1 2001 Mantem o instrumento t o seco e limpo como poss vel Limpe regularmente com um pano h mido macio Deixe o instrumento secar completamente antes de uso N o opera o instrumento em humidade extrema acima de 80 Evite condensa o deixando o equili brate de unidade a temperatura ambiental quando tomar o instrumento de um mais frio a um ambiente mais quente Permitir esfriar suficiente assegura que as aberturas do instrumento n o s o cobertas Informaci n Importante Spanish Si este equipo es utilizado en una manera no especifi cado por Hoefer S a la protecci n proporcionado por el equipo puede ser da ada Este instrumento es dise ado para el uso interior del laboratorio s lo S lo accesorios y partes aprobaron o suministraron por Hoefer S a puede ser utilizado para Operar para mantener y para atender a este producto Advirtiendo Porque este instrumento puede desar rollar voltaje y corriente suficientes para producir un golpe mortal el cuidado debe ser ejercitado en su operaci n Este instrumento es dise ado de acuerdo con el EN61010 1 2001 est ndar el ctrico de seguridad No obstante debe ser utilizado s lo por operarios adecuadamente capacitados Lea este manual entero antes de utilizar el instrumento y el uso s lo seg n las instrucciones El instrumento siempre debe ser utilizad
11. alue is Set Time Press either Adjust key once to go from Elapsed Time to Set Time On the right side of the display two red lights labeled Heater On and Vacuum On indicate the status of the heater and vacuum 2 Time Temperature key Toggles the LED display between showing time or temperature 3 Vacuum key Toggles the vacuum outlet On or Off A red light appears on the right side of the LED display when the vacuum is On 4 Adjust keys Adjust Set Temperature and Set Time reset Elapsed Time Press a key once briefly to move one interval Press and hold a key down to move in larger intervals Press both keys simultaneously to reset Temperature or Time to the Reset values 5 Reset values Lists the Reset values for Elapsed Time Set Time and Set Temperature Elapsed Time 0 00 Set Time OFF Temperature 40 C e p This declaration of conformity is only valid for the instrument when it is e used in laboratory locations e used as delivered from Hoefer Inc except for alterations described in the user manual and e connected to other CE labeled instruments or products recommended or approved by Hoefer Inc 3 Sp cifications Maximum Operating Ratings Wattage Pump Outlet Power requirements Environment Heater 800 W thermostatically controlled 575 W Model 80 6428 84 115 V 50 60 Hz 80 6429 03 230 V 50 60 Hz Operating environment Dimensions w x
12. between cellophane sheets A filter paper liner and a stainless steel screen lie under each stack A translucent rubber sealing sheet lies on top The gel type and thickness determine the cover sheet and layers used around the gel Fig 3 Gel drying stack for drying to cellophane Use this configuration for gels to be scanned imaged and stored Step 2 Prepare the gel drying stack The slab dryer accommodates both agarose and polyacrylamide gels The configuration of the gel stack layers depends on the next processing step and the thickness of the gel s Drying between cellophane sheets Immerse two sheets of porous cellophane in water e Lay one cellophane sheet smoothly on top of the filter paper liner Carefully center the gel on the cellophane Cover the gel with the second sheet of wet cellophane The stiff cover sheet produces a smooth gel surface that reduces scan irregularities Q Check that the edges of all the sheets fall within the recess of the platen If necessary trim the corners of the sheets to fit within the recess Cover the stack with the silicone rubber sealing sheet rubber sealing sheet stiff plastic cover sheet porous cellophane gel porous cellophane filter paper liner stainless steel screen Fig 4 Stacks for drying thin or low concentration polyacrylamide gels and agarose gels to filter paper for autoradiography e
13. e per mantenere e per revisionare questo prodotto Avvertendo Perch questo strumento pu sviluppare il voltaggio sufficiente e la corrente di produrre una scossa letale la cura deve essere esercitata nella sua operazione Questo strumento amp disegnato conforme mente all EN61010 1 2001 la norma di sicurezza elettrica Tuttavia dovrebbe essere usato degli opera tori solo correttamente addestrati Leggere questo manuale intero prima di usare lo strumento e l uso solo secondo le istruzioni Lo strumento deve essere sempre usato col piombo di terra della spina di alimentazione correttamente hanno messo a terra alla terra alla presa di corrente principale Usa il filo metallico e Vapparecchiatura solo intatti elettrici specifici per i voltaggi che lei usera Tutta Vapparecchiatura collegata all alto voltaggio dovrebbe essere conformemente a EN61010 1 2001 Tiene lo strumento come secco e pulito come possibile Pulire regolarmente con un morbido per spegnere il panno Lasciare lo strumento asciuga completamente prima dell uso Non opera lo strumento nell umidita estrema al di sopra di 80 Evitare la condensazione lasciando o piv l unit equilibra alla temperatura ambiente quando portare lo strumento da un pi freddo a un ambiente piu caldo Di permettere raffreddare sufficiente assicura che gli sbocchi dello strumento non sono coperti Viktig Informasjon Norwegian Hvis dette utstyret blir brukt i
14. e and maintenance on page 17 for an illustration of the mains power module Vacuum power cord receptacle Connects the vacuum pump to the vacuum timer 115 V 50 60 Hz Accommodates pumps that draw up to 5 A 230 V 50 60 Hz Accommodates pumps that draw up to 2 5 A 2 Unpacking the GD2000 Carefully unwrap all packages and compare the items received with the packing list making sure all items arrived If any part is missing contact your local Hoefer Inc sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or to use in case you need to return the unit Note We recommend a diaphragm vacuum pump such as the VP200 because the pump is chemically resistant to the liguids and vapors removed from the gel during drying A water aspirator or house vacuum is insufficient for gel drying Setting Up the Vacuum System The vacuum system must include a vacuum pump capable of moving an air volume of at least 1 5 m h but not more than 6 m h Rotary vane type pumps reguire both a chemi cal vapor trap and a cold trap set to maximum cooling to remove vapors that may damage the pump If you are using the VP200 Chemistry Diaphragm Pump no traps are reguired because the pump is constructed of chemically resis tant materials and is eguipped with two vapor trap
15. en mate ikke spesifisert ved Hoefer Inc beskyttelsen som ha blitt git av utsty ret kan bli svekket Dette instrumentet er utformet for innendors labora toriumbruk bare Bare tilbehor og deler godkjente eller forsynte ved Hoefer Inc kan bli brukt for drive vedlikeholde og betjene dette produktet Varsler Fordi dette instrumentet kan utvikle tilstrek kelig spenning og str m til produsere et d delig sjokk m bli vd bekymring i dets drift Dette instrumentet er utformet i samsvar med EN61010 1 2001 elektrisk sikkerhetsstandard Likevel burde bli brukt det bare av skikkelig utdannede oper at rer Les denne hele h ndboken f r bruking instru mentet og bruken bare gi til instruksjonene Instrumentet m alltid bli brukt med jorden blyet av kraftkabelen som riktig ha blitt jordet til jord p hovedledningen utl p Bruker bare uskadd elektrisk ledningsfremforing og utstyr som er spesifikk for spenningene du vil bruke All utstyr koplet til h yspenning burde v re i samsvar med EN61010 1 2001 Beholder instrumentet som t rker og rengjor som mulig Visk regul rt med et mykt fuktig stoff La instru mentet t rker komplett f r bruk Driver instrumentet i ekstrem fuktighet ikke ovenfor 80 Unng kondensasjon ved la enheten equili brate til omgivelsestemperatur ved taen instrumentets fra et kaldere til et varmere milj Til tillate tilstrekkelig kj lig sikrer at ventilasjon s pn
16. flasks A cold trap is optional but can be installed to control the amount of vapor released to the atmosphere Attach the vacuum tubing 9 mm 1 mm i d from the vacuum pump to the vacuum port on the GD2000 e Plug the vacuum pump either into the dryer control cabinet the power cord receptacle is on the left side of the control panel or into a lab power receptacle When the pump is plugged into the dryer control cabinet the vacuum timer automatically turns the pump on and off The 115 V model pump receptacle can accommodate any pump that draws less than 5 A The maximum rating for the 230 V model is 2 5 A If your pump exceeds this rating plug it into a lab receptacle and manually turn the power on and off ESSES Gel dryer controls The GD2000 control panel is located on the top of the gel dryer See Fig 1 A detailed view of the features of the control panel is shown in Fig 2 40 80 C 0 9 59 Hrs o o Po tm On O se Vacuum On Ei A o o Display EM Ce Fig 2 Gel dryer controls Elapsed JA 0 00 Set Time OFF Temperature 40 C e Vacuum On Off number control feature function 1 LED display Shows Set Temperature Set Time or Elapsed Time On the left of the LED display a light indicates whether the displayed value is C temperature or Hrs time When the displayed value is time and the LED colon blinks the value is Elapsed Time When the colon is not blinking the v
17. fly touching the sealing sheet over the gel Wet gels feel cold compared to the platen Typically the gel is dry when the drying surface has become evenly hot A markedly flattened gel contour also indicates that the gel has dried In automatic mode when the set time is reached the dryer beeps once and the heater turns off The vacuum power remains on for 10 minutes During this cooling down period the Elapsed Time display counts from C 00 to C 10 After ten minutes the vacuum power automati cally turns off and the dryer beeps once Step 6 Disassembly Remove each layer of the drying stack and clean the dryer according to instructions in Care and maintenance on page 17 e p15 Note When you manually preheat the dryer be sure the set time includes the time needed to prepare the gel stack as well as the time needed to dry the gel o pl6 Options for manual operations To apply a vacuum without heat Set the Temperature to 0 FF then set the timer Ten seconds after you set the time the vacuum starts without heat The vacuum stops when the Flapsed Time eguals the Set Time To preheat the dryer Set the Temperature and set the Time After ten seconds the vacuum starts Press the Vacuum key to turn off the vacuum and leave the heat on To restart the vacuum press the Vacuum key again 5 Care and maintenance Cleaning Turn mains power switch off and unplug the power cord e Remove
18. gebruikt die niet door Hoefer is gespecificeerd Nv de bescherming die door de uitrusting is verzorgd kan worden geschaad Dit instrument is voor binnenlaboratoriumgebruik enkel ontworpen Enkel onderdelen en delen keurden goed of leverden door Hoefer Nv kan voor het bedienen worden gebruikt handhavend en onderhouden van dit product Waarschuwend Omdat dit instrument voldoende spanning en stroom kan ontwikkelen om een dodeli jke schok te produceren moet zorg in zijn operatie worden geoefend Dit instrument is in overeenstemming met de EN61010 1 2001 elektrische veiligheidsstandaard ontworpen Niettemin zou het enkel door goed getrainde bedieningslieden moeten worden gebruikt Lees dit volledige handboek voor het gebruik het instrument en gebruik enkel volgens de instructies Het instrument moet altijd met de aardeleiding van het stroomsnoer correct grondde naar aarde aan het hoofdafzetgebied worden gebruikt Gebruik enkel onbeschadigde elektrische draad en uitrustings specifiek voor de spanningen u zult gebruiken Alle uitrustingen sloten aan aan hoogspan ning zou in overeenstemming met EN61010 1 2001 moeten zijn Houd het instrument zo droge en schone zoals mogelijk Bij Wis regelmatig met een zacht temper doek Verhuur het instrument droogt volledig voor het gebruik Bedien niet het instrument in extreme vochtigheid bovenstaande 80 Vermijd condensatie door het verhuren van de eenheid in evenwicht
19. h off Press Up again to count up in 15 minute intervals Press the Up and Down keys simultaneously to reset the time to O When the Set Time is set to O the display reads O FF When you have finished setting the time you can start the heater and vacuum o pl3 e pl4 To start the heater and the vacuum pump e You can press the Time Temp key to immedi ately start the heater and vacuum pump e If you don t press the Time Temp key ten seconds after you stop adjusting the time the heater and vacuum pump start automatically As the heater and vacuum pump start the timer begins to count Elapsed Time Hours Minutes The LED colon between the hours and minutes blinks each second when the timer is in Elapsed Time You may change the Set Time at any point after the LED begins to count Elapsed Time To change the set time while the heater is on Make sure the LED displays time The red light labeled Hrs is lit when the LED displays time Press the Time Temp key to toggle between temperature and time display The LED colon blinks when the display shows Elapsed Time e Press the Up or Down arrow key to change the display from Elapsed Time to Set Time Press the Up or Down arrow key to change the Set Time Note If using a cold trap with inline valves close the valve between the trap and gel dryer and then open the valve between the trap and the pump After the trap is pumped down
20. h si instrumentem ca kowicie wysuszy przed u yciem Nie prowadz do instrumentu w skrajnych wilgotno ci powy ej 80 Zapobiec kondensacji najmu przez jednostk r wnowa y do temperatury pokojowej przy podejmowaniu instrumentu z ch odniejsze w cieplejszych rodowiska Aby umo liwi wystarczaj ce ch odzenia zapewniaj e rozci cia of the instrument nie obj te ubezpieczeniem Informac es Importantes Portuguese Se este equipamento usado numa maneira n o espe cificada por Hoefer Inc que a protecc o fornecida pelo equipamento pode ser comprometida Este instrumento projectado para uso de interior de laborat rio s S acess rios e partes aprovaram ou forneceu por Hoefer Inc pode ser usada para operar manter e servicing este produto Advertindo Porque este instrumento pode desen volver voltagem suficiente e corrente produzir um choque letal cuidado deve ser exercitado em o pv sua Operac o Este instrumento projectado de acordo com o EN61010 1 2001 condic o de seguranca el ctrica N o obstante deve ser usado s por operadores adequada mente treinados Leia este manual inteiro antes de usar o instrumento e use s de acordo com as instrucc es O instrumento sempre deve ser usado com o chumbo de terra do cord o de poder corretamente baseou a terra nos canos sa da principais Usa fio el ctrico s intacto e equipamento espec fico para as voltagens que
21. ildade operat rer Las denna hela handbok f re anv nda instrumentet och anv nd bara enligt under visningarna Instrumentet m ste alltid anv ndas med jorden blyet av kraften repet riktigt grounded till jorden p det huvudutloppet Anv nder bara undamaged elektrisk trad och utrust ning specifik f r sp nningarna du ska anv nda All utrustning kopplats som till h g sp nning skulle vara i verensst mmelse med EN61010 1 2001 Haller instrumentet d torkar och reng r som m jlig Torka regelbundet med en mjuk fuktig trasa L t instrumentet torka fullst ndigt f re anv ndningen Fungerar inte instrumentet i extrem fuktighet ver 80 Undvik kondensering vid l ta enheten eguili brate till omgivande temperatur n r ta instrumentet fr n en kallare till en varmare milj Att till ta tillr cklig kyla ser till att halen av instru mentet inte t cks o pvi English Ea French R German R Ea Italian R Esa Spanish D Swedish R Waste Electrical and Electronic Eguipment WEEE This symbol indicates that the waste of electrical and electronic eguipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your eguipment Ce symbole indique que les d chets relatifs guipement lectrique et lectronique ne doivent pas tre jet
22. ingene av instrumentet er ikke dekket Wazne Informacje Polish Je eli ten sprz t jest wykorzystywany w spos b nie okre lone przez Hoefer Inc do ochrony przewidzianej przez urz dzenie mo e zosta obni ony Instrument ten jest przeznaczony do u ytku w labora toriach kryty tylko Tylko akcesori w i cz ci zatwierdzone lub dostarczone przez Hoefer Inc mog by wykorzystane do eksp loatacji utrzymania i obs ugi tego produktu Uwaga Poniewa ten akt prawny mo e by rozwini cie odpowiednich napi cie i bie cych do wyprodu kowania miertelnego szoku opiek musi by wykony wane w dzia aniu Ten instrument zosta zaprojektowany zgodnie z tym EN61010 1 2001 Bezpiecze stwo elektryczne standard Niemniej jednak nale y stosowa jedynie przez odpowiednio przeszkoleni operator w Znajd pa stwo to ca y podr cznika przed zastosowaniem instrumentu i stosowa jedynie zgodnie z instrukcjami Instrument musi zawsze by wykorzystane z ziemi doprowadzi do zasilania detonuj cego w a ciwie uzasadnione na ziemi w sieci wodoci gowej rynku zbytu Wykorzystanie tylko nieuszkodzona elektrycznych drut w i urz dzenia specjalne do napi zap ac wykorzystania Wszystkie urz dzenia pod czone do wysokiego napi cia powinny by zgodne z EN61010 1 2001 Kontrolowa instrumentu jako suche i czyste jak to mo liwe Wytrze regularnie przy pomocy mi kkiego wilgotnej szmatki Niec
23. iption The Hoefer GD2000 Slab Gel dryer rapidly dries acrylamide and agarose gels and perma nently bonds them to filter paper or transparent porous cellophane This is accomplished by heat ing the gel slab while simultaneously drawing away released moisture with an external vacuum pump The dryer accommodates one large 34 x 44 cm gel up to four standard 14 x 16 cm gels or twelve mini 8 x 10 cm gels Vacuum port Power cord receptacle and use drawer A clear silicone rubber sheet attached to the dryer with screws covers the gel drying stack Receptacle for vacuum pump power cord Control panel Fig 1 Features of the GD2000 Slab Gel Dryer e For a description of the dryer controls see page 5 e For diagrams of recommended drying stacks see Fig 3 on page 9 Fig 4 on page 10 and Fig 5 on page 11 Design Features Drying surface The PTFE coated cast aluminum platen is resis tant to acidic fumes that may be released while drying certain types of gels A grid of vacuum conduits on the surface allows released moisture to be pulled away Vacuum port Accepts vacuum tubing of 9 mm 3 8 1 mm i d The port for the external vacuum source is at the back of the instrument at the center of the platen Mains power The mains power module houses the power cord receptacle and one or two input fuses 115 V One F 12 A 250 V 3 AG fuse 230 V Two T 6 3 A 250 V 5 x 20 mm See Car
24. nen Maschinenbedienern verwendet werden Lesen Sie dieses komplette Handbuch vor dem Verwenden des Instrumentes und verwenden Sie nur gemaB den Instruktionen Das Instrument muss immer mit der Erdleitung der Macht Schnur richtig niedergelegt zur Erde am Hauptausgang verwendet werden Nurunbesch digte elektrische Leitung und Ausr s tung spezifisch f r die Stromspannungen verwenden die Sie verwenden werden Die ganze mit der Hoch spannung verbundene Ausriistung sollte in Uberein stimmung mit EN61010 1 2001 sein Das Instrument ebenso trocken halten und reinigen wie m glich Wischen Sie regelm ig mit einem weichen befeuchten Sie Stoff Lassen Sie das Instru ment trocken v llig vor dem Gebrauch Das Instrument in der u ersten Feuchtigkeit ber 80 nicht bedienen Vermeiden Sie Kondensation die Einheit equilibrate zur Umgebungstemperatur laBend wenn Sie das Instrument von einem k lteren bis eine w rmere Umgebung nehmen Um das gen gend Abk hlen zu erlauben stellen Sie sicher dass die Offnungen des Instrumentes nicht bedeckt werden Informazioni Importanti Italiano Se quest apparecchiatura usata in un modo specificato da Hoefer Inc la protezione fornito dall apparecchiatura potrebbe essere indebolita Questo strumento disegnato per l uso di laboratorio interno solo Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer Inc potrebbe essere usato per oper ar
25. o con el plomo de la tierra del cable de alimentaci n moli correcta mente a la tierra en la salida de red Utiliza alambre y equipo el ctricos s lo ilesos espec ficos para los voltajes que usted utilizar Todo equipo conectado al voltaje alto debe ser de acuerdo con EN61010 1 2001 Mantiene el instrumento tan seco y limpio como posible Enjugue regularmente con un suave el trapo h medo Permita que el instrumento seque completa mente antes de uso No opera el instrumento en la humedad extrema encima de 80 Evite condensaci n permitiendo la unidad equilibra a la temperatura ambiente al tomar el instrumento de un m s fr o a un ambiente m s tibio Permitir refrigeraci n suficiente asegure que las aberturas del Viktig Information Swedish om denna utrustning anv nds i ett s tt som inte har specificeras av Hoefer Inc skyddet tillhandah ll vid utrustningen kan skadas Detta instrument formges f r inomhuslaboratorium anv ndning bara Bara medhj lpare och delar godk nde eller levererade vid Hoefer Inc kan anv ndas f r fungera underh lla och servicing denna produkt varna D rf r att detta instrument kan utveckla tillr ck lig sp nning och str m att producera en d dlig st t m ste vas omsorg i dess funktion Detta instrument formges i verensst mmelse med EN61010 1 2001 elektriska s kerheten standarden Icke desto mindre b r det anv ndas bara av riktigt utb
26. or LED Display Check that the power switch is turned on Check that the dryer is plugged into a working receptacle Check the fuse s No heat Make sure time is set and the LED colon is blinking Make sure the temperature is not set to OFF If still no heat contact your local Hoefer Inc distributor for service No vacuum Make sure the tubing connects the vacuum port to the vacuum pump Make sure the vacuum pump is plugged into the vacuum receptacle on the dryer Check that time is set Vacuum automatically starts 10 seconds after time is set Check switches on the vacuum source Can t create vacuum seal Make sure the rubber sealing sheet seals around the entire inside edge of the recess Omit the mylar sheet and use plastic wrap on top of gel Check for tears or punctures in the rubber sealing sheet Replace it if necessary Gels crack Use thinner gels lt 0 75 mm if possible Thin gels rarely crack Reduce T Eguilibrate gels with 30 ethanol 2 glycerol for one hour before drying Make sure gel is completely dry before turning off vacuum Gels do not dry Do not use gt 5 glycerol during pre drying treatment Empty the liguid or cold trap Replenish dry ice in the cold trap Make sure to place only porous cellophane or filter paper under the gel Do not use plastic film or the stiff plastic cover sheet in the stack below
27. ques pour les tensions que vous utiliserez Tout 6guipement connect a haute tension devrait tre conform ment a EN61010 1 2001 Garder l instrument aussi sec et propre comme possi ble Essuyer r guli rement avec un doux touffer du tissu Laisser instrument seche compl tement avant l usage Ne pas fonctionner l instrument dans l extr me humidit au dessus de 80 Eviter la condensa tion en laissant l equilibrate d unit la temp rature ambiante en prenant l instrument d un plus froid un environnement plus chaud Permettre le refroidissement suffisant garantir que les conduits de l instrument ne sont pas couverts Wichtige Informationen German Wenn diese Ausr stung gewisserma en nicht angege ben durch Hoefer Inc verwendet wird kann der durch die Ausr stung zur Verf gung gestellte Schutz verschlechtert werden Dieses Instrument wird f r den Innenlaborgebrauch nur daf r entworfen Nur Zus tze und Teile genehmigten oder lieferten durch Hoefer Inc kann f r das Funktionieren das Aufrechterhalten und die Wartung dieses Produktes verwendet werden Die Warnung Weil dieses Instrument gen gend Strom spannung und Strom entwickeln kann um einen t dli chen Sto zu erzeugen muss Sorge in seiner Operation ausge bt werden Dieses Instrument wird in bereinstimmung mit dem EN61010 1 2001 elektrischen Sicherheitsstandard daf r entworfen Dennoch sollte es nur von richtig erzo ge
28. s Dette instrument er designet for indendors laboratori umbrug bare Bare tilbeh r og del godkendede eller forsynede ved Hoefer Inc kan m ske bruges for drive funktionsfejl og betjening dette produkt Advare Fordi dette instrument kan udvikle tilstraek kelig spaending og strom at fremstille et dodbringende chok skal pleje bruges i dets drift Dette instrument er designet i overensstemmelse med EN61010 1 2001 elektrisk sikkerhedstandard Alligevel skulle det bruges bare af passende traenede operatorer Laes denne hel h ndbog for bruging instrumentet og brug bare i henhold til instruktionerne Instrumentet skal altid bruges med jordblyet af netledningen rigtigt jordede til jord p hovedledning sudlobet Bruger bare uskadt elektrisk trad og udstyr som v re specifik for spaendingerne du vil bruge Alt udstyr forbundet til hoj spaending skulle vaere i overensstem melse med EN61010 1 2001 Beholder instrumentet s tor og ren som mulig Tor regulaert med et blodt fugtigt stof Lad instrument torken komplet for brug Driver ikke instrumentet i yderst fugtighed ovenfor o pii 80 Undg kondensation ved lade enheden eguili brate til omgivende temperatur ved tageen instru mentets fra et koldere til et varmere miljo Attillade tilstraekkelig afkoling forsikrer at lufthullerne af instrumentet er ikke daekket Belangrijke Informatie Dutch Indien deze uitrusting in een manier wordt
29. s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre quipement Dieses Symbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorgt werden d rfen sondern separat behandelt werden m ssen Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalit di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbricante Este s mbolo indica que el equipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener m s informaci n sobre la forma de desechar el equipo Denna symbol anger att elektriska och elektroniska utrustningar inte far avyttras som osorterat hushallsavfall och m ste samlas in separat Var god kontakta en auktoriserad tillverkarrepresentant f r information ang ende avyttring av utrustningen o pvii e 1 Gel Dryer function and descr
30. sin ennen k ytt Ei k yt v linett extreme ilmankosteudessa 80 n yl puolella V lt tiivistymist antamalla yksik n eguili brate ymp r iv n l mp tilaan kun ottaminen v line kylmempi l mpim mp n ymp rist n Sallia riitt v n j hdytt minen varmistaa ett v lineen ilmarei t peitet n Information Importante French Si cet quipement est utilis dans une mani re pas sp cifi par Hoefer Inc la protection fourni par V guipement pourrait tre diminu e Cet instrument est con u pour l usage de laboratoire int rieur seulement Seulement les accessoires et les parties ont approuv ou ont fourni par Hoefer Inc pourrait tre utilis pour fonctionner maintenir et entretenir ce produit Avertissant Parce que cet instrument peut d velopper la tension et le courant suffisants pour produire un choc mortel le soin doit tre exerc dans son op ration Cet instrument est conform ment con u a l EN61010 1 2001 norme de s curit lectrique N anmoins o piii il devrait tre seulement utilis par les op rateurs convenablement entrain s Lire ce manuel entier avant utiliser instrument et usage seulement selon les instructions L instrument toujours doit tre utilis avec l avance de terre du cordon d alimentation correctement a fond a la terre la sortie principale Utiliser le fil et l quipement lectriques seule ment intacts sp cifi
31. th the clear silicone rubber sealing sheet rubber sealing sheet Porous polyethylene cover sheet es gel thickness gt 1 5 mm filter paper filter paper liner stainless steel screen o pll Note The melting temperature of an agarose gel is dependent on its concentration and properties The drying temperature should not exceed the melting temperature We recommend a drying temperature of 50 for most agarose gels Note When the heater attains the temperature setting the red heater light goes off The heater light blinks when the heater comes on to maintain the set temperature o pl2 Step 3 Set the temperature The highest setting 80 C can be used for many types of gels for reliable rapid drying Use a temperature of 50 C for agarose gels Poly acrylamide gels prepared for fluorography may require a setting of 60 C to protect the fluors Follow the manufacturer s instructions If your gels tend to crack slower drying at a lower temperature setting may be indicated To set the temperature make sure the red light labeled C is lit If necessary press the Time Temp key to toggle between the time and temperature display e Use the Adjust keys to change the temperature You can set the heater to any temperature from 40 to 80 C or to OFF room temperature Press the Up or Down key once briefly to change by one degree Press and hold the key down to count fi
32. to the power entry module until it snaps into place o Plug the power cord in and turn the mains power switch on Insert a small screwdriver Use your fingers to pull blade in the slot Push in out the fuse drawer the direction of the arrow to release the fuse drawer Replacing the rubber sealing sheet Occasionally a tear or nick in the rubber sealing sheet SE1143 may inhibit the formation of the vacuum seal The rubber sealing sheet is attached to the dryer by four screws in a retaining bar along the edge of the platen To change the seal ing sheet you also need a small tube of clear silicone adhesive available at hardware stores Use a Phillips head screwdriver to remove the four screws on top of the retaining bar that holds the rubber sealing sheet in place e Lift off the retaining bar and the damaged rubber sealing sheet If necessary use a sharp edge to clean out any old silicone adhesive on the inside of the retaining bar Position the replacement sealing sheet on the platen aligning the four holes in the overlay with the four holes for the screws Q Lay a bead of clear silicone adhesive along the inside edge of the retaining bar Replace the retaining bar aligning the four holes of the retaining bar over the holes in the platen and sealing sheet Screw the retaining bar and sealing sheet in place o p19 6 Troubleshooting problem solution No power
33. ve 1 degree intervals followed by 5 degree intervals When the display reads 40 C press the Down key once to go to OFF When the temperature is 80 C press the Up key once to go to OFF Press the Up and Down arrows simultaneously to reset the temperature to 40 C To use the vacuum at room temperature turn the heater off The platen begins to heat at the same time that the vacuum pump starts 10 seconds after you have finished setting the time A red light next to the words Heater On indicates when the heat ing element is on Note Agarose gels become brittle when over dried Step 4 Set the timer The amount of time reguired for a gel to dry depends on such factors as gel thickness gel concentration drying temperature and vacuum applied A typical seguencing or 1 5 mm 10 T gel can be expected to dry in approximately 45 minutes at 80 C Larger gels may take 2 to 3 hours When dry the thickness of an agarose gel seen through the silicone flap decreases to about 1 mm Press the Time Temp key to go to the Time mode The LED display reads O FF and the LED colon blinks to indicate Elapsed Time e Press either the Up or Down key once to go from Elapsed Time to Set Time Press either the Up or Down key to change the Set Time Press Up once to set time for a continuous run On a continuous run the LED display reads r un and the heater and vacuum run continuously until you manually turn them bot
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