Hardware User`s Manual Automated 8
Contents
1. 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Automated 8 arm radial maze with photocells Panlab flicker ke 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A Due to the dimensions of the structure of the maze keep caution when assembling to avoid cuts and contusions B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing ha2. En representaci n del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canal s General Manager Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 30 04 2014 Automated 8 arm radial maze with photocells 25 Harvard Apparatus Panlab 6 Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment gt lt Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as
3. como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El simbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de aparatos usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente P y Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique gt lt Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt
4. MOUNTING THE RADIAL MAZE 1 Insertthe structure arm into the circular base using the corresponding orifices 3 y Figure 10 Inserting the arm in the circular base 2 Screw the Allen screws into the base to fix the structure arms Figure 11 Screwing the Allen screws 3 Secure the screws with a hexagonal key Figure 12 Securing the screws Automated 8 arm radial maze with photocells 14 Panlab H avala 4 Fit the platform with the 8 structure arms to the tripod Figure 13 Placing the platform on the tripod 5 Secure the platform to the tripod with a hexagonal key Figure 14 Securing the platform to the support 6 Place the maze arm in the structure arm and push it until it reaches the central area Figure 15 Placing the maze arm Automated 8 arm radial maze with photocells 15 Harvard Apparatus Panlab H 7 Secure the maze arm to the structure arm with the screw Figure 16 Securing the maze arm 8 Affixthe motor mechanism lid Figure 37 Introducing the maze arm sliding door Automated 8 arm radial maze with photocells 16 Panlab H avala 10 WORKING WITH THE EQUIPMENT WARNING Never place the animal in the centre of the maze before turning on the control unit otherwise the control unit will not be able to detect it When the control unit is turned on it balances automatically the load cell 10 1 MANUAL MODE 1 Assembly the maze as itis ex
5. PERSPEX CLEANING METALLIC PARTS TROUBLESHOOTING PREVENTIVE MAINTENANCE SPECIFICATIONS Automated 8 arm radial maze with photocells Harvard Apparatus 10 11 12 14 17 17 18 19 20 20 21 23 24 Panlab H 6 INTRODUCTION Mazes are commonly used in neuroscience An eight arm radial maze makes it possible to study animal spatial memory The animal s position in an LE 766 LE 768 eight arm radial maze is detected through infrared photocells in the arms and a load cell in the centre Figure 5 LE 766 eight arm radial maze There are two models of 8 arm automatic radial mazes CODE ANIMAL POSITION DETECTION LE 766 Photoelectric cells load cell LE 768 Photoelectric cells load cell Each arm in the radial maze has a door that is electrically controlled either in manual mode with a switch or in automatic mode with the Mazesoft 8 software Automated 8 arm radial maze with photocells Panlab H Jade ir 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT FRONT PANEL POWER BASE BEAMS DOORS MODE GROUP AZE CONTROL LE 766 768 Power Base Beams Panlab HARVARD APPARATUS Figure 6 Front panel e POWER This LED is on when the control unit is on e BASE This LED is on while the animal is in the maze s centre e BEAMS This LED remains on while an IR beam is cut by the animal e MODE Mode of control of the doors o MAN Manual mode In this mode the doors ar
6. connector used to connect the control unit to the computer serial port The software Mazesoft 8 shows animal position and is able to control doors when the control unit is set in REMOTE mode e RADIAL MAZE DB15 female connector The control unit is connected to the 8 arm maze through this connector The information of IR cells and the central load cell is transmitted to the control unit so that the Mazesoft 8 program is able to locate animal s position The signal to open or close the doors is transmitted through this connector too e POWER Power switch fuse holder and power inlet Automated 8 arm radial maze with photocells 10 Panlab 7 3 MAZE Figure 8 Eight arm radial maze The radial automatic maze has 8 arms and a central area Each arm has an automatic door The animal s position is detected through photoelectric cells in the arms and a load cell in the central area There is a cylindrical container for food at the end of each arm Automated 8 arm radial maze with photocells 11 Panlab A Harvard 8 EQUIPMENT CONNECTION Figure 9 Equipment connection Automated 8 arm radial maze with photocells 12 Harvard Pa n la b Apparatus The necessary cables and connections are listed in the following table FROM CABLE A 1A Maze s Platform 18 2A 28 3A 4A 4B 5B 6A 7A 8A 8B Computer s serial port Automated 8 arm radial maze with photocells 13 Panlab tenere 9
7. designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Automated 8 arm radial maze with photocells Panlab H avala 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with
8. 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instruc es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribui o para a protec o do ambiente 5 Automated 8 arm radial maze with photocells 26
9. Hardware User s Manual Automated 8 arm radial maze with photocells References LE766 76 0231 LE768 76 0232 Version Vo5 11 2014 Panlab s l u International Calls 34 934 750 697 Pan H Harvar d C Energ a 112 Domestic Call 934 190 709 EXORTUS 08940 Cornella de LL Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab H Ades ur 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be N exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C AN Warning about a metal surface that can supply electrical shock when it s A touched Decontamination of equipment prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEEE A
10. SPR16 class B ENVIRONMENTAL CONDITIONS Operating Temperature Operating Relative Humidity Storage Temperature 10 C to 40 C 0 to 85 RH non condensing to 50 C non condensing POSITION DETECTION Rat 30 gr Mouse 7 gr DOOR Time open closed 1 15 Regulation open Regulation closed Potentiometer P2 Potentiometer P1 DIMENSIONS CONTROL UNIT Width x Height x Depth Weight 230mm x 7omm x 280mm 2 42 kg ARM DIMENSIONS MODEL ANIMAL Width x Height x Depth DIMENSIONS CAGE asc DOOR WALLS TRIPOD Height Height Height Height L E766 144X345X715 1249X114 5 41 Dimensions are expressed in millimetres Automated 8 arm radial maze with photocells 24 Panlab H Ades ir DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direcci n del fabricante Energ a 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto AUTOMATIC RADIAL MAZE Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 766 LE 768 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the es
11. e controlled independently with the switches labelled 1 to 8 o REMOTE Remote mode In this mode the doors are controlled through the Mazesoft 8 program and the door switches are disabled o GROUP Group mode This mode allows opening or closing several doors simultaneously the doors that belong to the GROUP must have their switch set in the upper position then the control is done with the switch labelled GROUP e DOORS The function of these switches changes depending the mode of operation o MAN Manual mode In this mode if you raise the switch of door N this door will open and if you lower the same switch this door will close o REMOTE Remote mode In this mode the doors are controlled through the Mazesoft 8 program and these switches are disabled o GROUP Group mode The doors that belong to the group are selected by setting the switch in the upper position e UP DOWN This switch only is used in GROUP mode It controls all the doors that belong to the group In the upper position opens all the doors of the group in the lower position closes all the doors of the group The doors that do not belong to the group remain closed Automated 8 arm radial maze with photocells Panlab H avala 7 2 CONTROL UNIT REAR PANEL PC RS232 RADIAL MAZE POWER PC RS 232 Radial Maze Dnoooo G Dooooooo 0000000 Made in Spain by Panlab s l 110 220 V AC 50 60 Hz 40W Figure 7 Rear panel e PC RS232 DBg female
12. imal cut an IR beam the LED Beams will turn on 11 Once the experiment has ended remove the animal from the maze and clean it 12 Return to step 7 to conduct a new experiment Automated 8 arm radial maze with photocells 19 Panlab Msc dr 10 4 CLEANING PERSPEX To clean the Perspex pieces you can use a slightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove the foam with a wet cloth and finally dry them with a dry cloth WARNING To clean Perspex parts never use alcohol or alcohol derived products otherwise stripes will appear in the material 10 5 CLEANING METALLIC PARTS To clean the metallic parts you can use a slightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth Automated 8 arm radial maze with photocells 20 Panlab H Ades ur 11 IROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION Thecouipmientdcesnoustt un e Check the condition of the fuses Ensure that all cables are connected Check that control unit is turned on If you are using an USB to RS232 adapter check that drivers are Mazesoft 8 program is not detecting the correctly connected position of the animal Check that the COM port you have selected in Ma
13. in the position 5 To raise and lower the doors you must use the buttons in the program Mazesoft 8 6 Placethe animal in the centre of the maze 7 Follow Mazesoft 8 instructions to conduct an experiment 8 While the animal is in the centre of the maze the LED Base will stay on 9 Every time that the animal cut an IR beam the LED Beams will turn on 10 Animal position can be seen in the program Mazesoft 8 11 Once the experiment has ended remove the animal from the maze and clean it 12 Return to step 5 to conduct a new experiment Automated 8 arm radial maze with photocells 18 Panlab H avala 10 3 GROUP MODE 1 Assembly the maze as it is explained in chapter 9 2 Connectthe cables as it is explained in chapter 8 3 Turn on the control unit without the animal placed in the centre of the maze and the LED 9 Power will remain on the LEDS Base and O Beams must be off Remote Group 4 Setthe mode selector in the position 5 Select the doors you wish to control simultaneously by raising the switches SW ux labelled 1 to 8 Up 6 Place the GROUP switch POW in the DOWN position 7 Place the animal in the centre of the maze then the LED Base will turn on Up 8 Raise the switch DOWN to the UP position in order to open the selected doors the other doors will remain closed 9 While the animal is in the centre of the maze the LED Base will stay on 10 Every time that the an
14. plained in chapter 9 2 Connectthe cables as it is explained in chapter 8 3 Turn on the control unit without the animal placed in the centre of the maze and the LED 9 Power will remain on the LEDS Base and O Beams must be off Remote Man Ua Group 4 Setthe mode selector in the position 5 raise the door of any arm raise the switch of this arm Q 6 To lower the door of any arm lower the switch SY of this arm 7 Place doors in the initial state with the switches labelled from 1 to 8 Normally f Y 1 they are closed SY 8 Place the animal in the centre of the maze then the LED Base will light up SKYY 9 Open the doors by raising the switch 2 depending on the needs of the experiment 10 While the animal is in the centre of the maze the LED Base will stay on 11 Every time that the animal cuts an IR beam the LED Beams will turn 12 Once the experiment ends remove the animal from the maze and clean it 13 Return to step 7 to conduct a new experiment Automated 8 arm radial maze with photocells 17 Panlab H avala 10 2 REMOTE MODE 1 Assembly the maze as itis explained in chapter 9 2 Connectthe cables as it is explained in chapter 8 3 Turn on the control unit without the animal placed in the centre of the maze and the LED 9 Power will remain on the LEDS Base and O Beams must be off Remote Group 4 Setthe mode selector
15. s been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits A For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Automated 8 arm radial maze with photocells Panlab H PC Control Some of these instruments are
16. sential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques 2011 95 EC WEEE Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans 2006 42 EC les quipements lectriques et lectroniques ROHS Directiva mec nica Machinery directive Directive m canique Para su evaluaci n se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2010 EMC EN61326 1 2013 Class A Safety of machinery EN ISO 12100 2010 Consequently this product can incorporate the CE marking En consecuencia este producto puede incorporar el marcado CE C lt En cons quence ce produit peut incorporer le marquage CE
17. the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from the power inlet Automated 8 arm radial maze with photocells Harvard A Apparatus 2 Open fuse holder by pulling the flange with a regular screwdriver 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipment and contact technical service For electrical safety never open the equipment The power supply has dangerous voltages WARNING Automated 8 arm radial maze with photocells Panlab 5 TABLE OF CONTENTS 7 2 7 3 12 13 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION CONTROL UNIT FRONT PANEL CONTROL UNIT REAR PANEL MAZE EQUIPMENT CONNECTION MOUNTING THE RADIAL MAZE WORKING WITH THE EQUIPMENT MANUAL MODE REMOTE MODE GROUP MODE CLEANING
18. werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt PE Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente gt lt Nota em Protecc o Ambiental Ap s a implementac o da directiva comunit ria
19. zesoft 8 is the one where you have connected the control unit Check that BASE and BEAMS leds detect animal position Ensure that all cables are connected Check that control unit is turned on Check that control unit is set in REMOTE mode If you are using an USB to RS232 adapter check that drivers are correctly connected Check that the COM port you have selected in Mazesoft 8 is the one where you have connected the control unit Mazesoft 8 cannot control the doors Check that the jack stereo cable is connected One door does not open or closes Check that there is not any mechanical blocking of the door Check that control unit was not turned on after placing animal in the base Check that base is not touching Base LED is not detecting the animal Automated 8 arm radial maze with photocells 21 Panlab H jade ir O e Check that the maze is connected to the control unit e Loadcell is damaged Base LED blinks Automated 8 arm radial maze with photocells 22 Panlab H 12 PREVENTIVE MAINTENANCE EXPERIMENT CLEANING THE PERSPEX PARTS CLEANING THE METALLIC PARTS Ww CHECK THE CONNECTIONS Ww Automated 8 arm radial maze with photocells 23 13 SPECIFICATIONS Panlab Assis POWER SUPPLY Input voltage Frequency Fuse Maximum Power Conducted Noise Universal 100 Vacto 240 Vac 50 60 Hz 2 fuses 5x2omm 2A 250V Fast 40 W EN55022 CISPR22 CI
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