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AVASOFT for AvaSpec-102/256/1024/2048 version 6.2 USER`S

1. Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 81 Avantes website http www avantes com email Info avantes com antes 4 4 5 2 Irradiance Chart Display The irradiance chart displays the Mrs Ce ae graphics and output parameters S Power emiceed Tuuare pas that were selected in the settings op e 4 r 7 ir dialog Moreover up to ten a7 3 94 639 uate radiometric parameters can be oe 2 Energy a 389 0 to 780 0 ELLEI selected each with a user defined a ow y Expand Sine aed ram i Hone wavelength range The M a measurement results can be saved Ii a 12000 nnn and earlier saved spectra can be 5 som 5 None E Dd q Som graphically displayed and i Han compared to the measured e a Se a i re er eer aaran prn lt Pe spectrum The integration time and csm SPEE Fay Mame number of scans to average canbe AiE trom 2000 vo TOT em BOT changed to optimize the AD as FEE 34 482 mm Tescec WL 586 27 a Limindgas Flux ej 0 05361 Lumen 3 None Counts for each sample Invenaity 2 3511 Peak UL 566 20 Tii 10 Mane IntensityT 23 0760 The figure at the right shows the Cenvroad Wie 270 43 Em bu 572 215 nm Inkensiey 3 4815 result for a typical LED Sa wae Gas measurement inside an Avasphere If the light source is measured outside the sphere or with a cosine corrector and the radiant flux uw Watt radiant intensity uWatt sr luminous flux L
2. Fie Setup View Application Help start Wea SATI IA abl EN hiai average 1 Wavelength Inm s687 M frigger Delay 1000ns fiooo Note that the resolution of the delay in nanoseconds is steps of 41 67 ns with an offset of 41 67 ns So if you enter e g a delay of 800 ns the software will calculate the closest step 41 67 20 41 67 792 ns For the spectra that are saved in external hardware trigger mode a comment file will be created automatically in which the date time and delay are shown This may help to compare the spectra that were recorded with different delay settings to determine an optimal delay File Display Saved Graph The optimal delay period depends on the material and the laser in the LIBS application Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 39 Avantes website http www avantes com email Info avantes com antes 3 2 8 8 Setup Menu Options Auto configure Integration time After this menu option is clicked AvaSoft starts searching for an optimal integration time Depending on the maximum counts in the last scan the integration time will be increased decreased automatically until an optimal signal is measured The changing values of the actual integration time during the search can be followed in the integration time field at the edit bar If the auto configure integration time routine needs to be aborted before an optimal integration time has been found click the JAC bu
3. 4 Set up the experiment such that the other end of the fiber points at the sample of light to be determined use a fixture for the best results Usually some sort of spectrum may be seen on the screen but it is possible that too much or too little light reaches the spectrometer at the present data collection settings Too much light means that over a certain wavelength range the signal is overloaded shown as a straight line at any arbitrary height even near zero This can usually be solved by a shorter integration time The integration time can be changed in the main window in the white box below the start stop button If AvaSoft is collecting data the start stop button shows a red stop and the integration time box is gray indicating that it cannot be changed After clicking the stop button the data acquisition stops and the integration time can be changed The result of the changed integration time can be viewed after clicking the green start button Try to adjust the integration time such that the maximum count over the wavelength range is around 14000 counts When at minimum integration the signal is still too high fibers with a smaller diameter can be used When not enough light reaches the spectrometer likewise a longer integration time should be entered 5 When a good spectrum is displayed turn off the light source 6 Now save the Dark data This is done by File Save Dark from the menu or by clicking the black sq
4. After clicking the Channel button a dialog is shown in which the spectrometer channels can be selected for which data will be acquired and displayed The same result can be achieved with the option View Channel 5 Scope button By clicking the S button the data will be presented in Scope Mode The same result can be achieved with the option View Scope Mode Al Absorbance button By clicking the A button the data will be presented in Absorbance Mode The same result can be achieved with the option View Absorbance Mode T Transmission button By clicking the T button the data will be presented in Transmittance Mode The same result can be achieved with the option View Transmittance Mode L Irradiance button By clicking the I button the data will be presented in Irradiance Mode The same result can be achieved with the option View Irradiance Mode ii Autoscale Y axis button By clicking this button the graph will be rescaled on line A maximum signal will be shown at about 75 of the vertical scale The same result can be achieved with the option View Autoscale Y axis H Change Graph Scale button 10 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes By clicking this button a dialog will be shown in which the range can be changed for both X and Y axis This range can be saved as well and restored any time by clicking the Goto Preset
5. Process Control Digital Output Settings lolxl The box shows 16 values corresponding with a minimum and maximum threshold value for the 8 history channels All 16 values can be edited by History Threshold Values clicking them with the mouse In addition in the third column you can a A S select whether or not to invert the signal that is output For each channel eee poe e the minimum threshold value may not exceed the maximum threshold 2 3000 0 4000 0 M f occa value You cannot leave fields blank in that case enter 0 m After selecting OK the settings are written to a binary file called 5 5 E digital ini 6 o o E o 0 E The minimum and maximum threshold values are indicated in the history 8 o E graphs with horizontal lines pem en If Invert is not selected the corresponding output pin will be set high if the history channel output value lies between the minimum and maximum value It will be set low if the history channel output value exceeds the maximum value OR is smaller than the minimum value If Invert is selected the corresponding output pin will be set high if the history channel output value exceeds the maximum value OR is smaller than the minimum value It will be set low if the history channel output value lies between the minimum and maximum value istor Channel Functions Plies If you want to monitor both Po a ea threshold values you can assign two sai U cto
6. start button Try to adjust the integration time such that the maximum count over the wavelength range is around 14500 counts When at minimum integration the signal is still too high an attenuator a neutral density filter or fibers with a smaller diameter may be used When not enough light reaches the spectrometer likewise a longer integration time should be entered When a good spectrum is displayed turn off the light source 6 Now save the Dark data This is be done by File Save Dark from the menu or by clicking the black square on the left top of the screen with the mouse Always use Save Dark after the integration time has been changed 7 Turn on the light source again Save the present spectrum as a reference by choosing File Save Reference from the menu or by clicking at the white square next to the black one Always use Save Reference after the integration time has been changed Now the Transmittance reflectance T button or Absorbance A button spectra can be obtained online To have a better look at the amplitude versus wavelength the cursor button can be clicked A vertical line is displayed in the graph If the mouse cursor 1s placed nearby this line the shape of the mouse cursor changes from an arrow to a drag shape If this shape is displayed the left mouse button can be used to drag keep left mouse button down the line with the mouse towards a new position Moving this line shows the corresponding values of wave
7. 2004 Avantes S N 020800451 Fie Setup view Application Help no aS Stop History a Stop B wavelength Calibration J Integration time ms Color Measurement Wavelength nmn 658 61 Process Control d Absolute Irradiance Measurement Excel Output Enable Ox gen Settings Chemometr y 92 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com oVantes AvaSoft will write the data to three differently formatted sheets depending on user input in History Channel Function Entry and the Settings dialog You can enter the Settings dialog by selecting Application Excel Output Settings 4 6 3 Settings va Excel Automation E lel x Select Mode Export a fixed number of scans to Excel Continuous Mode Work book Select File Export Mode Scans to log fi 000 Interval fer Jo Ok Cancel First select the mode you want Export mode You can select Export Mode by selecting the top radio button In this mode a predefined number of scans will be logged to new worksheets that Excel will open Depending on your choice of history channel one of two formats of sheet or both will be opened by Excel If you select regular history channels without selecting View Spectrum the sheet will be formatted horizontally with the program adding a new row for each measurement
8. 2004 Avantes S N 031101451 File Setup View Application Help Gta Hardware OO a Wavelength Calibration Coefficients Intec Smoothing and Spline Wavelength nm 364 76 w Correct for Dynamic Dark Subtract Saved Dark Strobe enable 201 1 kHz enable DOr Options Check on Saturation Full width Half Max Integrals Autosave Spectra Periodically Correct For Drift a Automatic Save Dark by TTL shutter External Trigger Setting 4utoconfigure Integrationtine Use Custom Reflectance Reference Suppress Save Comments Dialog View Reflectance instead of Transmittance 3 2 1 Setup Menu Hardware This menu option displays a list of AvaSpec serial numbers that are Ma Select Spectrometer ox connected to the PC s USB port s and COM port s and which are not used by another instance of the application This option can be used to allocate a spectrometer to an application for example if one spectrometer is running with AvaSoft Full and another spectrometer needs to run with AvaSoft Raman software But it can also be used to run multiple spectrometers simultaneously just by restarting AvaSoft multiple times After clicking the OK button AvaSoft will communicate with the spectrometer serial number that has been activated in the dialog Available spectrometers 031101451 C o20800641 3 2 2 Setup Menu Wavelength Calibration Coefficients After clicking this option a dialog is shown in which the
9. A B E D F K L M Na 1 Time Elapsed msecs VVL1 F1 F2 WLS F5 WLG F6 07 03 2002 11 27 32 41252018 0 20 0438 5 Please do not enter your own 195 01 0 73522 formulas in this sheet 195 38 0 545509 7 Jas they will be overwritten 195 75 3 73522 8 by AvaSoft 196 12 3 45449 g 196 48 0 73522 10 196 85 0 545509 11 197 22 1 264778 12 197 59 2 545509 13 197 95 0 73522 14 198 32 5 545509 15 198 69 7 264778 16 199 06 4 545509 17 199 42 7 264778 18 199 79 12 54551 19 200 16 7 264778 20 200 52 11 54551 21 200 89 9 264778 22 201 26 10 54551 23 201 62 13 26478 24 201 99 13 54551 25 202 36 11 26478 26 202 73 11 54551 27 203 09 7 264778 28 203 46 11 54551 29 203 83 14 26478 30 204 19 12 54551 31 204 56 14 26478 32 204 93 16 54551 33 205 29 14 26478 34 205 66 16 54551 35 206 03 13 26478 4la l Pii Graphi AvaSoft Data Test sheet Gereed Num AvaSoft will check if a sheet named AvaSoft Data is present and will add this sheet if it is not present This way links to the sheet can be preserved between sessions The sheet contains the following data at fixed positions A3 Date Time stamp This is a floating point value in which the integer part is the day number starting at January 1 1900 with day 1 The fractional part represents a decimal time value where 0 5 is 12 00 h noon and 0 75 is 18 00 h This way differences in date time can be readily calculated by subtracting values
10. Amplitude at Centroid v Amplitude at Peak Detailed settings like the wavelength range and the radiometric output parameter for which the peak s are calculated can be done in the Irradiance chart dialog The Graphics setting determines which graph will be displayed in the irradiance chart dialog The Save Settings are used to enter the name of the textfile to which the results of the irradiance chart measurements will be saved By default this name 1s irrchart txt but by clicking the Change Output File button this filename can be changed Save Setting Save eiperiment results bo file jirchart tt Change Output File Save radiance Spectrum By enabling the Save Irradiance Spectrum option a complete irradiance spectrum will be saved together with the parameter results The name of the irradiance spectrum file will start with the filename that has been entered irrchart in the figure at the right followed by a sequence number and the extension irr The irradiance spectrum files can be saved and loaded from the irradiance chart dialog If all settings have been entered the measurements can be started by clicking the OK button To be able to measure the right A D counts corrected for the dark spectrum a warning will be displayed if a dark spectrum has not been saved Information o x G Dark data has not been saved For Master Block the light input then Save Dark by clicking the black button
11. Avantes website http www avantes com email Info avantes com antes The radiometric spectra can be saved and loaded by the Save and Open Saved Graph buttons see below under button bar Button bar Ext Pause Info Integration time ms et Sac average ho The Exit button will close the irradiance chart and return to the AvaSoft main Window The Pause button will stop data processing to be able to take a snapshot at the spectrum and or output parameters The Info button shows information about the current settings as shown in the dialog below v Irradiance Chart Measurement Info 2 Ioj j The last line in this dialog Save irradiance spectrum Yes refers to the save setting in section 4 4 5 1 If this option is enabled then all displayed parameters will be saved in this example to irrchart2 txt and the spectrum for the selected parameter in the radiometric graph will be saved to irrchart2xxxx irr in which xxxx represents the sequence number Hardware settings Spectrometer 021200652 Channel Master DetectorT ppe AvaSphere source inside sphere Diameter sampleport AvaSpere 10000 micron Calibration settings Intensity Calibration Filename 027 20065 2 M V15 UV600 1SP 50 dr Integrationtime during calibration 53 ms Smoothing during calibration 6 If the Save irradiance spectrum option is not enabled only the displayed output parameters will be saved G
12. If this menu option is preceded by a checkmark the strobe control function has been enabled To disable the strobe simply click the menu option when preceded by a checkmark 3 2 7 Setup Menu 1 kHz Enable DO2 Pin 2 of the high density 15 pole Sub D connector at the AvaSpec can be used to generate an 1 kHz signal This signal can be used to control an AvaLight LED light source in pulsed mode 26 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes 3 2 8 Setup Menu Options Check on Saturation Pull Width Half Max Integrals Gutosayve Spectra Periodically Correct For Drift Automatic Save Dark by TTL shutter External Trigger Setting Autoconfigure Integrationtime Use Custom Reflectance Reference Suppress Save Comments Dialog View Reflectance instead of Transmittance All options described in this section are standard included in AvaSoft FULL version In AvaSoft BASIC these features are not available 3 2 8 1 Setup Menu Options Check on Saturation The 14 bit A D converter in the AvaSpec results in raw Scope pixel values between 0 and 16383 counts If the value of 16383 counts is measured at one or more pixels then these pixels are called to be saturated or overexposed Since saturated pixels can disturb the measurement results a lot of attention has been given in AvaSoft and the driver package as161 dll to detect saturation and to noti
13. Settings Search Help Run Windows 2000 Professional Shut Gown After starting AvaSoft the dialog at the right Information will be shown to indicate that the USB connection has been detected a similar dialog will be shown if the serial RS 232 interface is used If more than one AvaSpec spectrometer is connected to your PC the dialog at the right will be shown which allows you to select the spectrometer serial number for which you want to use AvaSoft New in AvaSoft 6 2 is that you e gerne can run multiple spectrometers simultaneously just by restarting AvaSoft i multiple times 031101451 020800641 After clicking the OK button the main window is displayed Refer to section 2 for a description about the main window components A Quick Start can be found in section 1 if you want to start measuring immediately Detailed information about the menu options is found in section 3 Depending on the AvaSoft version Basic or Full and the extra add on modules that were ordered for your spectrometer up to eight applications are available in AvaSoft which are described in sections 4 1 to 4 8 History standard in AvaSoft FULL Wavelength Calibration standard in AvaSoft FULL Color Measurement add on module Irradiance Measurement includes AvaSoft Photon add on module Process Control add on module Excel Output add on module Oxygen add on module Chemometry add on module Aug 04 A
14. The include 2 order peaks option can be enabled if second or third order peaks should be added to the list of available literature peaks e g 507 30 nm as second order peak for 253 65 In most spectrometers the second order effects are eliminated by filters or coatings but if these options have not been added to the spectrometer and second order peaks are available then these can be included in the calibration The central column cells will be marked green if a second order peak is found and yellow if a third order peak is found AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc website http www avantes com 55 email Info avantes com avantes Press the Calculate button If your calibration is successful you will be asked to confirm new coefficients Select OK and the new calibration will be applied immediately At least 3 peaks are needed to successfully complete a new calibration Try to calibrate with more peaks Selecting too many peaks can however lead to peaks that cannot be matched with standard wavelengths 11 Points Used Calculated Calibration Coefficients are Intercept 3 501 35469030085E 002 Coefficient 1 2 86669699749683E 001 Coefficient 2 6 12149333338425E 006 Coefficient 3 1 89686889053452E 009 Coefficient 4 0 Q0000000000000E 000 St Deviation 0 0857 Range is From 350 nm to 8 amp 3 nm Do vou want to SAVE these Coefficients Cancel
15. antes 3 2 8 3 Setup Menu Options Integrals This utility can be used for measuring the Integral Range E 5 x total amount of energy coming into the Enter wavelength range and spectrum for integral calculation spectrometer Up to 10 integrals can be displayed simultaneously The inte gral Enable Spectrum Wavelength range Multiply with calculations are enabled after they have 1 Fe Master From 545 0 to 547 0 nm fi 000 been defined in the dialog that is shown at 4 TEE E GO i 4 7 Master From 521 0 to no nm hoo activating the menu option Setup Options f E _ Master From 521 0 to 591 0 nm 1 000 Integrals In the example at the right three ae fave ee de faye d the ai Tae Master From 521 0 to 591 0 nm 1 000 integrals gt i gt H Master From 521 0 to 591 0 nm 1 000 marked by the Enable checkbox in the e C Mee o Fomo tofo nm fioo first column In the second column a 9 O ee Fomo tofo nm foo spectrum can be chosen out of all spectra 10 Maste x Froms21 0 tofo nm fioo that are at that moment displayed in the main window in AvaSoft A full list of the spectra to choose from this includes earlier saved spectra like integral 3 in the i J OK X Cancel example is displayed after clicking on the arrow button at the right side of the second column In the third and fourth column the wavelength range can be entered over which the integral should be calculated F
16. This setting determines when AvaSoft should switch channels during the measurements If set to Software the user determines when to switch channels The dialog below will be shown after the OK button has been clicked in the FOS Settings dialog As long as the Reference channel is the Active FOS 34 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Channel the data processing does not occur in the main window but a small graph as shown in the figure below shows the A D Counts measured at the FOS reference channel When clicking the Sample channel radio button the FOS 2 switches channels and the corrected data will be processed as usual A small window will remain visible in which the FOS channel can be switched back to the FOS reference channel and measure a new reference spectrum Active FOS Channe i Sample channel f Reference channel Elapsed time since last FOS channel switch 0 00 38 400 500 600 ro B00 Wavelength nim If set to Auto switching of channels will be done after a preset number of seconds f Continuously measure sample update FOS reference on switch Furthermore you can select if you want to update Continuously update FOS reference measure sample on switch the FOS reference spectra once every x seconds or Ieee semen 60 seconds only take one sample measurement once every x seconds In figu
17. antes After an intensity calibration has been performed or loaded the Irradiance Time Series TAB becomes available in the Irradiance Settings dialog By clicking the TAB the Irradiance Time Series settings are displayed With time series irradiance measurements up to 8 radiometric parameters can be followed simultaneously in a graph against time The functions F1 to F8 can be selected by clicking the corresponding TAB sheet at the top of the Time Series settings dialog Furthermore a number of general function independent parameters can be entered below of the function TAB sheets The following features can be set separately for all functions F1 to F8 v lrradiance Settings O x Calibration Inadiance Chart Itadiance Time Series Fi F2 r3 r4 rs re r7 re Fz Channe _5 Colorimetric Parameters CIE Standard Observe Enable OE Cu C Dominant Wav 2 degrees Master Cy C Comp Dom Way C Disable ci C Color Temp Purity 10 degrees Hardware setup Radiometric Parameters Wavelength Range Detector type C pWwatt ont poule r C pMol n C wJoule sr From 327 37 nim f CC UVAVIS or Fiber C pat fr C pMol s n C pMol C pwatt fe C AvaSphere C pouledcr pMol s C pWatt sr poule fe To 11001 nm Photometric Param Peak Parameters Wavelength Range C Lux C Center wavelength Center intensity C FWHM C Lumen fr e i From 327 37 nm C Ce
18. shown after the History Function Entry option has been A val cates chosen X axis time Y axis Function Value C Auto Fixed C Auto Fixed The functions F1 to F8 can be ea a ge econds ss selected by clicking the Display Last Minutes ein e ax corresponding TAB sheet at the E ARRUN top of this dialog l Display No Graphics To Speed Up Data Processing Furthermore a number of z ave Function Output general function independent C Do not save Function Output Save every Seconds Change Output File b d Save Function Output To File D source S W 5 Delphi4 dataHistory dat parameters can be entered teas Backus Fis below of the function TAB sheets X Cancel Save HCF Load HCF Function Type The first time that the Function Entry dialog is activated all 8 functions are not activated Function Type None To define a function the Function Type radio button needs to be changed from None to Integral User Defined View Spectrum or Peak After defining the function type the Measure Mode Function Definition and Function Display Settings can be set 46 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes The four possible modes Scope Absorbance Transmittance or Irradiance are described in section 3 3 1 to 3 3 4 Note that if the selected measure mode is Absorbance Transmittance or Irradiance a reference and dark spectru
19. the photon energy at 250 nm and at 1000 nm is E 250 6 626 x 10 2 998 x 10 250 10 7 946 x10 Joule photon E 1000 6 626 x 10 2 998 x 10 1000 10 1 986 x10 Joule photon leV 1 60207 x 10 Joule so the photon energy expressed in eV photon becomes E 250 7 946 x10 1 60207 x 10 4 9592 eV photon E 1000 1 986 x10 1 60207 x 10 1 2398 eV photon Suppose we measure 20 uWatt cm at a certain wavelength 20 uWatt cm 20 pJoule s cm 0 2 Joule s m 2 1 60207 x 10 eV s m 1 248 10 eV s m 5 Knowing the photon energy at 250 nm and at 1000 nm from 3 and 4 the number of photons that correspond with 20 uWatt cm at 250 nm and at 1000 nm can be calculated from 5 by for 250 nm photons 1 248 10 4 9592 2 517 10 photons s m for 1000nm _ photons 1 248 10 1 2398 1 007 10 photons s m With 1 mol 6 02308 x10 Number of Avogadro 1 umol 6 02308 x10 So the number of photons expressed in umol s m when measuring 20 uWatt cm at wavelength 250 nm and also 20 uWatt cm at 1000 nm becomes for250nm 2 517 10 6 02308 10 0 418 umol s m7 for 1000nm 1 007 10 6 02308 10 1 672 umol s m In the table below the radiometric parameters that can be measured in AvaSoft are listed Note that a wavelength range needs to be specified over which the parameter spectral output will be integrated In the first co
20. the scope data is corrected with the saved dark 3 2 6 Setup Menu Strobe Enable DO1 This option can be used to enable or disable an external strobe e g the XE 2000 attached to an AvaSpec Spectrometer The XE 2000 needs to be attached to the AvaSpec by connecting an IC DB15 2 interface cable to the high density 15 pole Sub D connectors at the AvaSpec and XE 2000 If the XE 2000 has the possibility to switch between Single Flash and Multi Flash in that case there will be a switch at the backside of the XE 2000 make sure that the switch is always in the Single Flash position when using the XE 2000 with an AvaSpec spectrometer in Single Flash mode the XE 2000 is triggered at pin 1 in Multi Flash mode at pin 2 The measured light intensity of the XE 2000 is o independent of the integration time in AvaSoft To Number of Pulses a x increase light intensity the number of pulses per integration interval should be increased When clicking the Strobe Enable menu option a dialog is shown in which this number of pulses can be set The maximum ao frequency at which the XE 2000 operates is 100 Hz This means that the minimum integration time for 1 pulse per scan is 10 ms When setting the number of pulses e g to Lancel 3 the minimum integration time becomes 30 ms It is recommended to keep the integration time as low as possible to avoid unnecessary increase of noise Enter the number of pulses per integration interval
21. then additional lines will be written to the textfile test sat If time series measurements color measurements irradiance measurements are saved to file the data will also be checked on saturation and if saturation occurs additional lines will be written to a logfile with the same filename as the measurement results but with the extension sat e g History sat The files with extension sat can be openend with any textfile editor for example Notepad Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 29 Avantes website http www avantes com email Info avantes com antes 3 2 8 2 Setup Menu Options Full Width Half Max The Full Width Half Maximum of a peak is the bandwidth in nanometers for which the intensity is higher than half of the maximum intensity of that peak The FWHM can be calculated in Scope or Irradiance Mode This utility is used mostly measuring the output of laser diodes During FWHM calculations the intensity needs to be corrected for the dark data Therefore TEETE x it is recommended to enable the option Subtract Saved T E E E E EERTE Dark as shown in the dialog at the right which appears after 1 Setup Substract Saved Dark has been enabled Use the clicking the FWHM option After clicking the OK button eee ae cee the Full Width Half Max values are calculated for a number of peaks To mark a peak for which the FWHM values need to be calculated press the ALT key and click with the left mouse bu
22. to be saved at the FOS reference channel as well as at the FOS sample channel This option requires an equal integration time for FOS reference and sample channel The following data needs to be available Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 33 Avantes website http www avantes com email Info avantes com antes o Dark spectrum at FOS sample or reference channel o Reference spectrum FOS sample channel o Reference spectrum at FOS reference channel The difference of these two methods of correction is that in the first method there is no relation between the data measured at both channels Avasoft uses only the differences measured in time at the FOS reference channel to correct the data measured at the sample channel In the second correction method the correction factor between the both FOS channels is determined first and then the white reference measured at the FOS reference channel is recalculated and saved as a new reference for the FOS sample channel Since for both methods a dark spectrum needs to be available save dark before enabling the Correct for Drift by FOS 2 INLINE UV VIS menu option After clicking the menu option the following dialog will be shown FOS Settings reference channel fae E Integration time me for FOS reference channel 15 000 fi U Mr of scans to Average for FOS reference channel 14 000 13 000 Save Dark for FOS reference channel 12 000 11 000 S
23. which would be much more difficult if this was a text representation B3 Time in milliseconds after midnight This value does not have a one millisecond resolution Remember that Windows is not a real time operating system It can however be used as a reasonably accurate indicator of the time that passes between scans C5 C Wavelength scale in nm for first History Channel spectrum D3 Value for first History Channel 0 if spectrum is selected D5 D Spectrum for first History Channel 96 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes E5 E Wavelength scale in nm for second History Channel spectrum empty if spectrum is not selected F3 Value for second History Channel F5 F Spectrum for second History Channel empty if spectrum is not selected etc depends on the wavelength range selected 4 6 4 Start Output You start the output the usual way with Application History sw5_ooi O x Start measuring You can also use the corresponding button on the Button Bar x Call was rejected by callee Please do not perform large alterations of the worksheet while data is being transmitted An error Call was rejected by callee will be issued if Excel is too busy Moving around the worksheet should not pose any problems Save any alterations to your worksheet before starting the transfer
24. 101 x Actual Points Actual Line a New Points New Line Actual Calibration Filename Master Load Read Absorbance for point 1 Calculate New Calibration Line Calibration Points pae pe e m 1 Calibration Line Coefficients Intercept Sa Coef 1 Ps Coef 2 SS zj St Dev SS The unit of concentration can be entered in the Conc Unit field This is just a text no adjustments will be made if you change this value Enter the known concentration values for the available standards in the central column of the calibration points table You can include the reference sample with zero concentration in these measurements Aug 04 Avantes AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc website http www avantes com 103 email Info avantes com ayantes After this the dialog contains for ill chemometry se E iol x example the data shown in the figure p Select Variables l Actual Points at the right Channel Master Actual Line 5 a New Points If the zero concentration reference Peakat ieo nm a m New Lins sample is included like in the ate o onc Unit l l example at the right set up the a experiment to measure this ae 0 reference Use the keyboard or Stat New Caitraton Clear Data _ E i i Calibration Points Calibration Line Coefficients First Order 7 Linear Default mous
25. 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 4 4 4 Perform Intensity Calibration If a calibrated light source such as the AvaLight HAL CAL or AvaLight DH CAL is available an intensity calibration can be performed By selecting the menu option Application Absolute Irradiance Measurement a window is shown in which the settings for the intensity calibration become visible after clicking the Perform Intensity Calibration button The following settings need to be entered before starting the intensity calibration e Spectrometer channel Calibration Lampfile e CC UV VIS Fiber or AvaSphere sample port diameter Perform Intensity Calibration Perform Intensity Calibration Spectrometer Channe Masher Evas pech 20001 20 DH Imp view Edit Lamp Data CL UY VTS Fiber or AyvaS phere sampleport diamete fi ond micron Start Calibration Save Calibration For a single channel spectrometer the spectrometer channel will always be set to Master For multichannel spectrometer systems the spectrometer channel for which the intensity calibration will be performed can be selected from the list Master Slavel Calibration Lamp The energy output in 1 Watt cm nm for the calibration lamp that will be used can be found in a file with the extension Imp This file needs to be selected by clicking the Select button Select Lamp Calibration Fi
26. 2 INLINE UV VIS is a 2 channel fiber optic switch Switching between the optical channels can be done manually or by a TTL signal see operating manual or FOS 2 INLINE UV VIS hardware manual By connecting an IC DB15 2 interface cable between the AvaSpec and FOS 2 or an IC DB15 FOS2 2 if a shutter needs to be controlled as well from the AvaSpec the spectrometer can control the position of the switch In AvaSoft the bottom channel has been defined as reference channel while the top channel should be connected to the optical path that is used to measure the samples The Correct for Drift with the FOS 2 can be done in two different ways 1 By regularly switching to the FOS reference channel the measured spectrum can be compared to the saved FOS reference spectrum The differences can be used to correct the data that is measured at the FOS sample channel To be able to correct the sample data the following data needs to be available o Dark spectrum FOS sample channel o Dark spectrum FOS reference channel if integration time at reference channel is different from integration time at sample channel o Reference spectrum at FOS reference channel 2 In Transmittance Reflectance or Absorbance mode the measured spectrum at the FOS reference channel can be used as a new reference for the FOS sample channel To be able to correct for the difference in sensitivity between both FOS channels the reference material white tile or blank needs
27. After starting the History Channel Measurements the peak in the spectrum drawn for F1 will be marked by a red vertical line immediately In the next 3 seconds the spectral peak may vary in height or position but the red vertical line remains at the same place and height After 3 seconds the red vertical line becomes orange and a new red line will be drawn at the position and with the height of the peak detected at that moment t 3 After 6 seconds another line 1s drawn and the colors are shifted again so that red is most recent then orange then green At t 27 seconds the 10 vertical line is drawn again red for the most recent the oldest is now represented by a light gray color At t 30 seconds the oldest peak is removed from the list since in this example the Nr of peaks to display is 10 a new one is added and the colors are shifted according to the definition etc Function Definition Peak Function Definition Spectrometer Channel Wavelength range nm Peak Outpu Master From 455 0 To 45710 i Wavelength Intensity In case a function 1s defined to display a peak versus time the following parameters can be set The Spectrometer Channel The default is Master but in case more channels are available this can be changed to Slavel Slave2 up to Slave7 The from and to edit boxes may be changed to specify the wavelength range in nanometer which will be evaluated in the peak search Finally a selection
28. Change Output File button Last option in the Save Function Output box is to enable or disable the possibility to create a backup file during the measurements If this option is enabled AvaSoft will create a backup file with the same filename but with the extension bak also in ASCII This backup file is updated every scan and can be used in case the filename that has been selected has failed to save the data for instance because of a power failure during the measurements Save HCF Load HCF buttons AvaSoft automatically saves all parameters function definition save options to the file hcf ini and restores these parameters the next time AvaSoft is started With the Save HCF and Load HCF buttons it s also possible to save these parameter settings to and load from a file with the extension hcf With this feature it is possible to save for each experiment a different HCF file so it can be loaded a next time the same settings are required The left figure below shows the dialog in which the name of the hcf file can be entered after clicking the save HCF button The right figure below which is displayed after clicking the Load HCF button illustrates how to select an earlier saved HCF file Enter History Channel Function Definition Filename Opslaan als E speed c p experiment 1 hzt experiment 2 hef experiment 3 Act New hct Bestandsnaam experiment 4 Opslaan als
29. Found New Hardware USB device dialog and starts z Universal Serial Bus controllers searching for the driver this can take a few minutes 4 Avantes Spectrometer board Depending on the Windows version the Files Needed USB Root Hub YIA USB Universal Host Controller dialog at the right may come up which allows Files Needed x you to browse to the directory where the USB driver is located Click the Browse button and E o a a browse to the directory AR C WINNT SYSTEM32 DRIVERS and double click the AvsUsb sys driver Then click the OK button in the Files Needed dialog at the right to complete the installation Type the path where the file is located and then click Ok Copy files from 7 The AvaSoft software can now be started and ee will detect the spectrometer at the USB port If not please have a look at the Device Manager which should contain the Avantes Spectrometer board under Universal Serial Bus controllers as illustrated in the figure above Don t hesitate to contact us if the problem remains unsolved Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 109 Avantes website http www avantes com email Info avantes com
30. Reset When selecting this option the graph will be reset to the default X and Y axes 3 3 8 View Menu Autoscale Y axis By using this option the graph will be rescaled on line A maximum signal will be shown at about 75 of the vertical scale 3 3 9 View Menu Goto Preset Scale By clicking this menu option the scale for X and Y axis will be set to a range that has been set before The same result can be achieved by clicking the Goto Preset Scale Button in the button bar 44 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes With the Grid Enable option activated a grid will be displayed in the graph as shown in the figure below 3 3 10 View Menu Grid Enable 3 3 11 View Menu Progress Bar Enable If using long integration times or a high number of averages it can take a few or more seconds before a new scan is received by the application To get an indication about how much time it will take until the next scan arrives a progress bar can be displayed After enabling the progress bar by clicking the menu option it will be displayed after the next scan has arrived The progress bar will be shown only if the time between scans is more than one second The time between scans is roughly the integration time multiplied with the number of averages However if the number of averages is high the time between scans can get longer because of the overhead
31. Scale button see below The menu option with the same functionality is View Change Graph Scale Goto Preset Scale button By clicking this button the scale for X and Y axis will be set to a range that has been set before The same result can be achieved with the menu option View Goto Preset Scale bt Graphic Reset button By clicking this button the X and Y axis will be reset to their default values The same result can be achieved with the option View Graphic Reset Jacl Auto Configure Integration time button After this button is clicked AvaSoft starts searching for an optimal integration time Depending on the maximum counts in the last scan the integration time will be increased decreased automatically until a scope signal of about 14500 counts is measured During the search routine the changes in integration time can be followed in the integration time edit box in the edit bar A dialog will display the new integration time if the search is ready The routine can be aborted by clicking the button again before the search has ended a H C F button The History Channel Function button allows you to switch directly to the history channel function screen to start measuring immediately Of course first the functions need to be defined Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 11 Avantes website http www avantes com email Info avantes com antes The two buttons below are available only if AvaSoft has bee
32. Select Channel Present Standard At hy A Master 365 08 365 01 0 07 Connect the fiber to the AvaLight CAL light source and to the spectrometer channel to be calibrated Choose a suitable integration time so as not to saturate the detector The peaks can be seen in scope mode O x Aug 04 Avantes Select the menu option Wavelength Calibration Perform New Calibration The auto calibration routine will now search for a number of peaks initially it will look for 5 peaks in the data from the Master channel The spectrometer channel and the number of peaks to look for can be altered A new search can be performed by pressing the Find Peaks button The peaks are shown in 3 columns The first column shows the position of the peaks found The second column shows the position of a suggested standard peak if available The last column lists the difference between the first two columns You can edit the values of Peaks i Max Fit Order W include 2nd order peaks 2 2 4 Find Peaks Calculate Close the second column by selecting them with the mouse Select the polynomial order In most cases a third order polynomial will show an excellent fit see figure above 404 60 407 71 455 87 546 09 577 10 576 98 696 46 750 44 763 49 772 41 404 66 407 78 435 64 546 08 579 07 696 54 750 39 763 51 772 40 0 06 0 07 0 03 0 01 0 14 0 09 0 08 0 05 0 02 0 01
33. because of drift in the lightsource will be used to correct the data of the other selected spectrometer channels The wavelength range over which the data can be corrected will be the overlapping wavelength range between reference spectrometer channel and the spectrometer channel to be corrected If this option is activated a dialog is shown in which the reference channel and one or more depending on the number of spectrometer channels that are available channels to be corrected can be selected After selecting the right setup and clicking the OK button AvaSoft shows the following information Turn on your light source choose the right integration time and save reference Then turn off the light source and save dark After clicking OK AvaSoft enables the spectrometer channels that are relevant in the correct for drift application After saving the reference and dark files a message dialog shows that the data will be corrected for drift The menu option will be preceded by a checkmark To disable the correct for drift option the menu option if preceded by a checkmark needs to be clicked 3 2 8 6 Setup Menu Options Automatic Save Dark by TTL shutter To use the automatic save dark option an interface cable needs to be connected from the spectrometer to the light source with shutter AvaLight HAL S AvaLight DHc AvaLight D H S DUV The interface cable between TTL shutter and spectrometer is a 15 to 15 pin cable IC DB15 2 The T
34. between AQ File Menu absorbance and concentration AQ Setup Menu AQ View Menu A c l i NE Application Menu History Where A is the absorbance or extinction sis the extinction coefficient of the compound to be measured c is the concentration and 1 is the optical path length In practice this relationship is only linear at reasonably low absorbance levels say less than 2 The wavelength at which the absorbance is measured must of course be kept constant AQ WaveLenath Calibration AQ Color Measurement AQ Absolute Inradiance AQ Photon AY Process Control Excel Qutput AQ Oxygen w Chemometry To take concentration measurements you will need a spectrometer with either a cuvette holder or a dip probe and a suitable light source jeee Introduction 2 Quick Start AvaSoft Chem can display and save the calculated concentration in two ways a Enable The concentration can be displayed on line in a separate display window 2 Settings for up to eight spectrometer channels if available E Wass anan anlant sa ta naht bhinatase nhananla that ana dianlare and anrea Instead of browsing through the contents to a specific topic the search TAB can be selected as illustrated in the figure below After typing in a keyword e g concentration and clicking the List Topics button a list of all topics containing this keyword is shown By selecting a topic at the left double click the informati
35. binary graphic file as shown below Extension binary file Extension text file ROH TRT ABS TAT TRM TTT IRR TIT All text files start with a header with information for the graphic file that has been converted The header shows the comment line the integration time the number of scans that has been averaged the number of pixels used for smoothing the serial number of the spectrometer that was used to save the data The data in a TRT file is given in two columns The first column gives the wavelength in nanometers the second one the scope data The data in the TAT TTT files is presented in five columns The first column gives the wavelength in nanometers The second to fourth column give respectively the dark reference and scope data The fifth column shows the calculated value for absorbance in a TAT file or transmittance in a TTT file The format for the irradiance text files TIT depends on the availability of AvaSoft IRRAD The absolute irradiance text file that will be generated if AvaSoft IRRAD is available shows six columns The first two columns are the wavelength and dark data The third column is the transfer function from counts to microwatts The fourth column contains the measured counts In column five and six the calculated irradiance values are given in microwatts per square centimeter column 5 and in photon counts column 6 The relative irradiance text file that will be generated if AvaS
36. can be made to define the Peak Output Wavelength or Intensity If both wavelength and intensity need to be followed against time one of the functions F1 F8 can be used to follow the peak wavelength and another function to follow the peak intensity To visualize the peak changes a third function can be set to View Spectrum with e g the wavelength range set to 455 457 as well and the nr of peaks to display set to 10 In the figure above the resulting output will be the wavelength of the highest peak between 455 and 457 nanometer If the intensity button had been selected the function output would have been the intensity of the highest peak between 455 and 457 nanometer Function Display Setting ans Function Value Auto tf Fixed C Seconds Min 95 00 Display Last i f Minutes C Hours Max 105 0 For Integral User Defined and Peak functions the output can be displayed graphically against time The amount of time that will be displayed at the X axis can be set manually by clicking the Fixed radiobutton If set to Auto the time axis will be set to 1 minute This option is not available if the View Spectrum Function Type has been selected because the X axis range is in that case in nanometers and determined by the wavelength range as described above The Y Axis can also be set to Fixed or to Auto The Auto option will set the Y axis range to the minimum and maximum function values that are in the list of measured datapo
37. determines which parameters will be shown in the irradiance chart By clicking one of the group parameters the detailed settings for this group become visible A description about these parameters and how they are calculated can be found in section 4 4 1 inchart twt Change Output File Save lradiance Spectrum By default only the Colorimetric parameters are enabled Colorimetric Parameters CIE Standard Observe Dominant Wavelength 2 degrees Tou Complementary Dom Wavelength Color Temperature Purity 10 degrees The parameters that are enabled in the figure above will be displayed in the Irradiance Chart The detailed setting for the radiometric parameters is done while measuring in the irradiance chart dialog If the hardware setup is not source inside sphere you can already select if you want to measure only radiometric parameters received at the diffuser surface or also calculate the power and or energy emitted by the source An important assumption when calculating the emitted power or energy is that the source should be an isotropic point source Radiometric Parameters j Calculate Power Energy Photon Counts received by detector surface Calculate Power and or Energy emitted by a source leotropic pointsource assumed Distance from source to detector can be set while measuring Radiometric Parameters and wavelength range can be selected while measuring If the hardware set
38. displayed on line in a separate display window for up to eight spectrometer channels if available You can select up to eight history channels that can display and save concentration values against time This application can be combined with the Excel and Process Control applications 4 8 1 Quick Start How to make concentration measurements with AvaSoft Chem Start the AvaSoft software and click the Start button in the main window Set up the absorbance experiment with a cuvette holder or a dip probe measuring the sample with the lowest concentration usually your reference Switch on the light source 3 Adjust the integration time to get a good reference signal with a maximum around 14500 counts The easiest way to do this is by using the AutoConfigure Integration time option 4 Adjust the number of averages The higher the better however the time for a single absorbance reading must remain practical If you want to change it first press the Stop button and press the Start button again after changing the Average value in the Edit bar Switch off the light source and save a dark spectrum switch it back on and save a reference 6 Change to Absorbance mode by clicking the A button in the button bar Measure the absorbance of a sample with a high concentration to find the wavelength and bandwidth of an absorbance peak to use most of the time the wavelength with the highest absorbance is used 7 Calibrate your absorbance
39. features Zoom in select a region to be expanded to the full graph To select this region click the left mouse button in the white graphical region and drag it downwards and to the right After releasing the left mouse button within the graphic display both the X and Y axis will be rescaled to the new values of the selected region Zoom out drag with the left mouse button within the white rectangle but in stead of dragging the mouse downwards and to the right drag it in another direction After releasing the mouse button both the X and Y axis will be reset to their default values Move X Y dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right Move Y if a mouse wheel is available on the mouse being used then the spectrum can be moved up or down by moving the mouse wheel 2 5 Status bar Master File AVANTESOOUT AOH Amplitude 3990 3 Smoothing Spline 0N For each selected spectrometer channel a statusbar at the bottom of the main window shows information about the file to which the data will be saved amplitude at current wavelength and the current settings for the smoothing and spline parameters The field at the right of the Spline setting is used to indicate that the spectrometer is receiving too much light at a certain wavelength range 16383 counts before correcting for dynamic dark smoothing or averaging in which case the label saturated will become visible Se
40. hardware setup for which the calibration lamp has been calibrated CC UV VIS cosine corrector bare fiber or integrating sphere should match the hardware setup that is used during the intensity calibration The diameter of the cosine corrector 3900 micron bare fiber or AvaSphere 6000 10000 or 15000 micron for respectively AvaSphere 30 AvaSphere 50 and AvaSphere 80 needs to be entered in micron It is important that the hardware setup that is used during the calibration is the same as the hardware setup in the ir radiance measurements 4 4 4 1 Start Intensity Calibration i Integration time ms f Sad Average ho Smoothing E Verify that the calibration is ON for at least 15 ayes minutes and that the hardware has been setup 16 000 correctly Then click the button Start DEN Calibration ea 13 000 As aresult the Scope data A D Counts for the pee selected spectrometer channel will be displayed Bin graphically gn00 Set the smoothing parameter to optimize io smoothing for the fiber slit diameter used see 8000 also section 3 2 3 5 000 4 000 Set the integration time such that a good 3 000 reference signal maximum around 14000 2 000 15000 counts is measured It s also possible to ii 3 let AvaSoft search for an optimal integration r w a a a oe a a time by clicking the AutoConfigure Integration Diii time JAC button Set the number of scans to Average to a high number to r
41. in a TRT file absorbance in a TAT file transmittance in a TTT file or irradiance in a TIT file 3 1 13 File Menu Convert Graph to Select one or more graphic files to convert to J CAMP J CAMP o E qJ amp Ale hefdata La AVANTESOOOGB ROH a AVANTESOOIZROH fa AVA JCAMP DxX is a standard file format for i AVANTESOOO ACH BAe le AVANTESOOIS AGH ba aval a AVANTESOOO2 ROH a AVANTESOOOS ROH fa AVANTESOO14 ROH fa AVAL exchange of spectra between different AR a AVANTESO00SAOH bal AVANTESOOIS ROH fa AVAL spectrometer systems and computers A ba AVANTESOOO4 ROH a AVANTESOOIO ROH fa AVANTESOOIG ROH fa AVAL JCAMP DX spectrum is a text file which a AVANTESOOOS ROH AVANTESOOT1 ROH AAA a aa can be viewed corrected and annotated 5 with a text editor A lot of other elaborate spectrometer software programs like Objectnaam J AVANTESOOT7 ROH AVANTESOOO AO Grams32 and OPUS are capable of Objecten van hetiype Granh Scope Mode DEED importing files in JCAMP DX format P The J Camp format requires an equal distance between the data points at the X axis wavelength Converting the CCD pixels to wavelength points is not a linear function so before writing to the J Camp format the 2048 for an AvaSpec 2048 data points are linearized first This option requires that graphic files were saved earlier by using the option File Save Experiment After choosing this option a window s
42. is scope so the extension of the earlier saved spectra is roh AVANTESOOO1 ROH E AVANTESOOO2 ROH ja AVANTESOOOS ROH a AVANTESOOO4 ROH a AVANTESOOD6 ROH a AVANTESOOO ROH To select graphic files that were saved in another measure m g rbance click TE ode Ve absorba ae Object name AVANTESOO0S ROH AVANTESOOO2 ROH gt Open My Network F behind the Graph Objects of type Graph Scope Mode Cancel Mode and pick the desired A measure mode 20 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes To select graphic files from another folder or drive click i behind the current foldername If a graphic file is marked by a single mouse click on the filename the comment line for this file appears at the top of the graphical region in the main window Selecting multiple filenames can be realized by using the CTRL or SHIFT key in combination with the mouse If the CTRL key is pressed all the files that are clicked by the mouse will be selected for conversion If the SHIFT key is pressed all the files in between two clicked files will be selected for conversion Select the name of the file s to be converted to ASCII and click the Open button To leave this dialog without converting files click the CANCEL button The extension of the text files depends on the extension of the
43. is used to illuminate the reference sample To correct for the drift in the system it needs to be measured first This can be done with a 2 channel Fiber Optic Switch FOS 2 INLINE UV VIS in which case one channel is connected to the reference sample and the other channel to the sample that needs to be measured By regularly switching channels manually or automatically the measured deviation from 100 at the reference channel can be used to compensate the measured data at the sample channel The same principle can be used if a 2 or multiple channel spectrometer is available In that case the sample and reference data can be measured simultaneously and the measured data at the reference sample can be used directly to correct the sample data Disadvantage of the correct for drift by spectrometer channel is that the data for reference and sample are measured by using two optical benches which may react differently on temperature changes If the drift is mainly caused by the light source such as a difference in flash intensity of the XE 2000 this method of correction is recommended The advantage of the correct for drift by FOS 2 INLINE UV VIS is that it will also correct for drift caused by temperature changes of the optical bench Disadvantage is that the correction is sequentially so not every scan is immediately corrected Moreover there needs to be time available to switch channels Correct for Drift by FOS 2 INLINE UV VIS The FOS
44. nie ET will return values lt 16383 counts The other detector types in the AvaSpec 102 256 and 1024 don t show this effect and don t need to be Log saturated pixel areas to file on Save Measurement corrected for this Normally you also don t need to use this third level for the AvaSpec 2048 but M Cancel when measuring a peaky spectrum with some Log saturated pixel areas to screen Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 27 Avantes website http www avantes com email Info avantes com antes heavily saturated peaks the autocorrect can be used To illustrate this a strong peak from the AvaLight CAL calibration line source was heavily saturated at 435 84 nm This caused the most heavily saturated pixels to return inverted lt 16383 counts pixel values figure below at the left In the right figure the saturation detection has been set to the third level which will not only detect the saturated pixels at 16383 counts but also detect and correct the inverted saturated pixels Disadvantage of the autocorrect detection level is that processing the saturated scans by the application takes more time saturated Master channel Scope Yalues saturated Master channel Scope Values Saturation Notification If the saturation detection has not been switched off the spectrometer channel statusbar will show the text label saturated as long as one or more pixels of that spectromete
45. of the calibration file can be entered Type in the name you will use for this calibration line MyFirst in the figure at the right then click the Save button As a result the file MyFirst cal will be created and the calibration line will become the actual calibration line that will be Save in chem E used to calculate the concentration TE My Computer levels for the specified spectrometer channel Master in this example File name MyFirst save as type Calibration files call Cancel Zi After a calibration line is saved it will become the actual calibration line which is blue colored This actual line will be used for concentration v Chemometry Settings E 0 x calculations Furthermore the peer EET P anne Mast Y Actual Li Calibration Line coefficients are Master a New Ports a 7 Peak at New Li shown in the lower right corner of oe fr i l 10 nm the dialog and the predicted OO E concentration values that have been calculated by using the calibration Stat New Caibraion Clear Dats o o2 og 06 08 coefficients have been added for the 1 1 AMA ote Eo cleats Calibration Points Calibration Line Coefficients 7 standards into the right column of the First Order Linear Default a onc onc table Second Order Quadratic ical a TT TE iniarcapt 7 9811E 001 l o0 0 Cot e7 By compar
46. photometric equivalent for the Radiant Flux Watt is the Luminous Flux expressed in Lumens The photometric equivalent for the Radiant Intensity uWatt sr 1s the Luminous Intensity expressed in Lumens sr This unit is equal to Candela The photometric equivalent for Irradiance 11 Watt cm is called Illuminance expressed in Lumens m This unit is equal to Lux Since the geometry of the three categories is the same for radiometry and photometry the same can be written about the hardware setup luminous flux can be measured inside an integrating sphere When measuring a source at a certain distance from the integrating sphere or cosine corrector the luminous flux can be calculated assuming that the source is an isotropic point source The Luminous Intensity Candela and Illuminance Lux of a sphere can be measured outside the integrating sphere or with a cosine corrector 74 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 4 4 2 Quick Start Quick Start 1 Perform absolute irradiance measurements using a calibrated lamp l d 3 10 11 12 Start the AvaSoft software and click the Start button in the main window Connect a fiber to the Spectrometer input port Start the Absolute Irradiance Application software by clicking the menu option Application Absolute Irradiance Click the Perform Intensity Calibration butt
47. releasing the mouse button both the X and Y axis will be reset to their default values Move X Y dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right 66 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes 4 4 Applications Absolute Irradiance Application Irradiance Chart Measurements Exit Pause Info i Hl Integration time ms po Sad Average ho M Radiometry Power emitted uUVatt Y CIE 1931 2 degrees standard observer Chromaticity Diagram IRRCHART0001 IRR red led Edit il IRRCHARTOQOZ IRR green led oe GaSe eee ees eee eee yee 1 Power e 550 0 to 750 0 i i 93 916 pWatt 44 4 1 2 2 Power e 500 0 to 700 0 o i i 93 603 pWatt 400 500 600 700 y Expand Size Wavelength nm 3 None 16 000 eS en eee 14 000 i t oe oe a ate a 12 000 1 t E 53 10 000 o pote peso ine re aban Nannon 5 None 6000 t H 4 000 1 r COLLIE abet al aba 15 6 None 0 ni 0 2 0 3 0 4 05 0 6 0 7 0 1 x 400 500 600 700 Wavelength nm j a None M Colorimetry Peak Measurements Photometry Sample Color Ea Evaluate highest peak x 0 568 from 500 to z nm 8 None y 0 425 FWHM 36
48. series experiment has been ended as described in section 4 1 3 History Display Saved History Graph Function Independent Parameters Save Function Output The results of a time series color experiment will be saved to an ASCII file if the radio button Save Function Output has been selected A number of seconds between saving can be entered for data 64 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes reduction in case measurements are carried out over long periods Entering a value of zero results in saving every scan The name of the file to which the data will be saved can be changed after clicking the Change Output File button Last option in the Save Function Output box is to enable or disable the possibility to create a backup file during the measurements If this option is enabled AvaSoft will create a backup file with the same filename but with the extension bak also in ASCII This backup file is updated every scan and can be used in case the filename that has been selected has failed to save the data for instance because of a power failure during the measurements Save Setting Load Settings buttons AvaSoft automatically saves all parameters color parameters save options to the file cootime ini and restores these parameters the next time AvaSoft is started With the Save Settings and Load Settings buttons it s also poss
49. set to zero AvaSoft will save the spectra as fast as possible x Cancel e Number of scans to save the number of spectra that needs to be saved can be entered On top of the parameters that appear at the right a checkbox shows Save As Fast As Possible no screen updates If the white checkbox in front of this text line is marked the Automatic Save option will always save the number of scans that have been entered as fast as possible To do so the time delay between scans is automatically changed to 0 milliseconds at the moment the checkbox is clicked With this option selected the spectra will be saved 5 to 10 times faster compared to if this option is not selected This speed increase has been achieved by mainly two reasons 1 During the saving of the number of scans that has been entered the screen update which is very time consuming is not activated Instead the main window is temporarily minimized and the number of scans to save is counted down in a new dialog 2 Nocomment files will be generated Other factors that have a positive effect on the speed of the data acquisition and which can be set in AvaSoft are e Smoothing parameters e Integration time e Averaging e Number of pixels to transfer section 3 2 2 Furthermore it is recommended to keep the number of files in your experiment directory small a few hundred maximum because a large number of files in the same experiment directory have a negat
50. shown below Extension Mode RCM Scope Mode ACM Absorbance TCM Transmittance Reflectance ICM Irradiance Example suppose the name of our experiment is avs Then saving one spectrum in scope mode one in absorbance mode and two in transmittance mode results in the following files avs0001 roh spectrum data in scope mode avs0001 rcm comments for the spectrum saved in avs0001 roh avs0001 abs spectrum data in absorbance mode avs0001 acm comments for the spectrum saved in avs0001 abs avs0001 trm spectrum transmittance mode avs0O001 tcm comments for the spectrum saved in avs0001 trm avs0002 trm spectrum data in transmittance mode avs0002 tcm comments for the spectrum saved in avs0002 trm Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 17 Avantes website http www avantes com email Info avantes com antes After leaving the application and opening AvaSoft the next time saving graphics in scope absorbance and transmittance mode will then result in respectively the data files avs0002 roh avsO002 abs and avs0003 trm as well as the comment files avs0O002 rcm avs0002 acm and avs0003 tcm Before saving the name of the graphic file is displayed in the statusbar at the bottom of the screen After saving the sequence number is automatically incremented by one Saving graphic files if multiple spectrometer channels have been enabled If graphic files are saved while more than one channel is viewed at the same
51. the AvaSpec 2048 from 30 ms at full wavelength down to 14 ms for a small selection of 10 pixels A second advantage is data reduction because only the spectral data will be saved at the pixels for which the wavelength is in the specified wavelength range 3 2 3 Setup Menu Smoothing and Spline The Cubic Spline Interpolation Algorithm can be applied to get a MA Smoothing and Spline l ES better estimation for the spectral data between the pixels on the detector arra spline Smoothing Iw Master fi Master Smoothing is a procedure which averages the spectral data over a Iv Slavel fi Slave number of pixels on the detector array For example if the smoothing parameter is set to 2 the spectral data for all pixels x on X Cancel the detector array will be averaged with their neighbor pixels x Xn 1y Xnt1 aNd Xn 2 Cubic Spline Interpolation In the figure at the right the effect KATERE of spline interpolation is illustrated M MSS SATII mam The Master data shows the AD counts for 4 pixels connected by a straight line linear interpolation The Slavel data is for these 4 pixels exactly the same as for the Master data but this time the cubic spline interpolation algorithm has been applied resulting in data which is smooth in the first derivative and continuous in the second derivative The spline interpolation can be useful for applications in which the output of line sources like laser Siol File LEJAO0
52. this calculation is that the source should be isotropic and the distance between diffuser and source should be greater than five times the largest dimension of the source approximation of point source e Radiant Intensity uWatt sr The radiant intensity is the optical power per unit solid angle It is used to quantify the optical power that is emitted by a source into a certain direction Radiant intensity 1s calculated from the measured irradiance by multiplication with the square of the distance between source and diffuser surface It is assumed that the source is a pointsource Irradiance uWatt cm Irradiance is used to measure the power that is received by a surface Radiometry Radiometric measurement can be done in different setups like with fiber optic cosine corrector or integrating sphere Both setups can be used to measure the irradiance spectrum received at the surface of the diffuser cosine corrector or integrating sphere sample port at a certain distance from a light source When measuring at a certain distance from the source the radiant intensity and flux can be calculated as described above When measuring a light source inside an integrating sphere the radiant flux can be measured but radiant intensity and irradiance parameters cannot be measured Radiometric parameters calculated from the power distribution The power distribution can be easily converted in an energy distribution by multiplying the power with the int
53. type Function Definition Files Annuleren Bestandsnaann Experiment 1 hcf Bestandstypen Function Definition Files Annuleren Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 51 Avantes website http www avantes com email Info avantes com antes After the definition of one or more functions the OK button is clicked to confirm the CANCEL button to leave the dialog without changes If the OK button is clicked AvaSoft performs a number of checks on the data that has been entered If no warnings show up the parameters that have been entered are accepted and the measurement can be started by the History Start Measuring menu option 4 1 2 History Application Start Measuring This option displays the output against time for the history functions that have been defined in the History Channel Function Entry dialog box If the option Display no graphics to speed up data processing has been marked in the function entry dialog the function output will be shown by numbers only which are updated each time a new scan is saved to the output file There are six buttons at the top of this window an Exit button a Pause Start button a Save Reference button a Save Dark button an Info button and a Print button If the red Exit button is clicked the time measurements are ended and the main window and menu is activated again The yellow Pause button can be used to stop the time measurements temporarily After cli
54. values described before Rows and 2 contain the date time stamp and the elapsed millisecond value as described in the previous section Column A of the first sheet holds the wavelength scale The wavelength range matches the wavelength range as specified in the History Channel Functions The lower left half of the Settings dialog can be used to enter the number of scans you want to export to Excel and the time interval in seconds between two scans you want to log Default values are 1000 scans and no interval meaning as fast as possible Continuous Mode You can select Continuous Mode by selecting the bottom radio button In this mode data will be written to a single sheet each scan overwriting the previous one In the lower right part of the Settings dialog you can select the workbook the sheet will be added to thereby enabling you to use your own calculations and graphs in Excel on live data from AvaSoft Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 95 Avantes website http www avantes com email Info avantes com antes Press the Select File button to select the workbook Please save an empty workbook using Excel if you don t have a previous workbook to open EEA SOY atx Ed Microsoft Excel continuous xls 6 Bestand Bewerken Beeld Invoegen Opmaak Extra Data Yenster Help la x lO eRe ARY tte n A SM e gt aria 10 B Z UES 3H 8 E o Oo A Q19 M
55. wavelength calibration coefficients can be changed manually Background The wavelength A that corresponds to a pixel number pixnr in the detector in the spectrometer can be calculated by the following equation Intercept X1 pixnr X2 pixnr X3 pixnr X4 pixnr in which Intercept and X1 to X4 correspond to Intercept and First to Fourth Coefficient in the figure below For example if we want to calculate the wavelength at pixel number 1000 using the numbers in the figure at the right the wavelength becomes Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 23 Avantes website http www avantes com email Info avantes com avantes Wavelength Calibrati Eel x A 384 054 0 136492 1000 Miwon i i I 6 71259E 6 1E6 Spectrometer Channe f Master i Slavel 5 66234E 10 1E9 51 3 267 nm First Coefficient 0136452 Process data only when in The Restore Factory Settings button Second Costficient 6 712596 06 folowing wavelength range restores for all spectrometer channels the Third Coefficient 56624E 10 eee 400 at Hees a Fourth Coefficient 0 00000E 00 stop at au nm tnat were saved to the uring factory calibration Gi 384 054 x Cancel Restore Factory Settings The Process data only when in following wavelength range option can be used to transfer only a limited number of pixels from the spectrometer to the PC This can significantly speed up the transfer time e g for
56. written Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 93 Avantes website http www avantes com email Info avantes com 4 Microsoft Excel Blad1 antes BAIMENA ax Bestand Bewerken Beeld Invoegen Opmaak Extra Data Yenster Help x Oseba 6hy see r 4 i 10 B Zz UF 82 Bile o A Al r Time A B D H MM N F 1 Time lElapsed msecs F1 F2 F7 8 2 3 07 03 2002 11 32 34 41553984 0 16 65232 0 0 0 0 D 0 4 07 03 2002 11 32 35 41554535 O 21 97153 D 0 0 0 0 0 5 07 03 2002 11 32 35 41554775 O 15 77341 0 0 0 0 D 0 6 07 03 2002 11 32 35 41555095 0 14 52785 0 0 0 0 D 0 Z O7 03 2002 11 32 35 41555386 0 15 37158 0 0 0 0 D 0 8 07 03 2002 11 32 36 41555696 0 15 29554 0 0 0 0 0 0 9 07 03 2002 11 32 36 41556007 0 18 36336 0 0 0 0 D 0 10 07 03 2002 11 32 36 41556317 0 11 52971 0 0 0 0 D 0 11 07 03 2002 11 32 37 41556618 0 15 38815 0 0 0 0 D 0 12 07 03 2002 11 32 37 41556928 O 14 91846 0 0 0 0 D 0 13 O7 03 2002 11 32 37 41557239 O 11 53747 0 0 0 0 0 0 14 07 03 2002 11 32 38 41557539 0 22 45974 0 0 0 0 D 0 15 07 03 2002 11 32 38 41557850 0 18 35447 0 0 0 0 D 0 16 07 03 2002 11 32 38 41558160 0 19 09817 0 0 0 0 D 0 17 07 03 2002 11 32 38 41558460 0 13 05762 0 0 0 0 D 0 18 07 03 2002 11 32 39 41558771 0 12 70234 0 0 0 0 D 0 19 07 03 2002 11 32 39 41559091 0 17 02149 0 0 0 0 D 0 20 07 03 2002 11 32 39 41559382 0 16 68021 0 0 0 0 0 0 21 07 03 2002 11 32 40 41
57. 07 ROH Aarglibade 594 5 Smoother 0 Soine ON Masto File LEJAQODE ROH Amolade 583 7 Sercothers 0 Soine OFF 24 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes diodes is displayed or for other applications which require a high resolution Note that for the AvaSpec 2048 with 2048 pixels the effect of spline interpolation is not visible if the data is shown at full scale The monitor resolution is much less than 2048 pixels The effect of spline interpolation can only be visualized if the number of detector pixels that are displayed is smaller than the number of monitor pixels at the x axis Smoothing To get a smoother spectrum without losing information it is important to set in the software the right smoothing parameter The optimal smoothing parameter depends on the distance between the pixels at the detector array and the light beam that enters the spectrometer For the AvaSpec 2048 the distance between the pixels on the CCD array is 14 micron Light beam Smoothing With a 200 micron fiber no slit installed connected the optical pixel resolution is about 14 3 CCD pixels With a smoothing parameter set to 7 each pixel will be averaged with 7 left and 7 right neighbor pixels Averaging over 15 pixels with a pitch distance between the CCD pixels of 14 micron will cover 15 14 210 micron at the CCD array Using a fiber diameter of 200 micron means that we w
58. 2 History Application Start Measuring 4 1 3 History Application Display Saved History Graph 4 2 Applications Wavelength Calibration 4 2 1 Calibrate Wavelength Application Perform New Calibration 4 2 2 Calibrate Wavelength Application Restore Original Calibration 4 3 Applications Color Measurement 4 3 1 Color of an Object Background 4 3 2 Menu option Color Measurement 4 3 2 1 LABChart 4 3 2 2 Time Series 4 4 Applications Absolute Irradiance Application 4 4 1 Background 4 4 2 Quick Start 4 4 3 Load Intensity Calibration 4 4 4 Perform Intensity Calibration 4 4 4 1 Start Intensity Calibration 4 4 4 2 Save Intensity Calibration 4 4 5 Irradiance Chart 4 4 5 1 Irradiance Chart Settings 4 4 5 2 Irradiance Chart Display 4 4 6 Time Series Measurement 4 5 Applications Process Control Application Digital Output signals Using the Process Control Application in AvaSoft 4 5 1 4 5 2 4 6 Applications Excel Output 4 6 1 Select Source Data 4 6 2 Enable Excel Output 4 6 3 Settings Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Avantes website http www avantes com 3 email Info avantes com 36 40 40 4 4 42 42 42 42 43 44 44 44 44 44 45 45 46 46 46 52 53 90 90 90 92 92 92 93 4 6 4 Start Output antes 4 6 6 Limitations and Optimization Notes 4 7 Oxygen application 4 7 1 Quick Start How to take oxygen measurements with AvaSoft Oxy 4 7 2 Enabling the applica
59. 2475 2 ite500 ave sel avantes0007 roh W saoothing 1 graphs are displayed at the top of the graphical region together with information about amplitude at current wavelength amp integration time it and smoothing s settings at the moment that the file was saved and the name of the graphic file If the active spectrometer channels e g Master have not been unselected with the View Channel option the actual data for the activated channel s will be displayed in the same graph as the selected graphic files By clicking the green start button the online measurements can be compared directly to the graphics that were saved before ls Master Pile AVANTESOOUELROH ldumelibude 1908 1 Smoothing 2 Spina on The menu option File Display Saved Graph is preceded by a checkmark as long as the earlier saved graphics are displayed To clear these earlier saved graphics select again the menu option File Display Saved Graph after which the checkmark disappears and only the spectra for the active spectrometer channel s will be displayed 3 1 11 File Menu Convert Graph to ASCII This option requires that graphic files were saved earlier by using the option Look jr Say data 020800641 Select one or more graphic files to convert to ASCII ajx ra rE E File Save Experiment After choosing this option a window shows all files in the current measure mode In the example at the right the measure mode
60. 257 13 2394104 13 52955627 12 49716949 9 722677502 769216156 11 91 13 71450806 12 83588409 15 2769165 10 65768433 12 99326324 9 794944763 10 19 13 18588257 11 2394104 14 52955627 8 497 169495 12 7228775 10 69216156 8 919 15 71450806 13 83588409 14 2769165 6 657684326 14 99326324 11 79494476 11 19 13 16566257 15 2394104 14 52955627 12 497 16949 12 7228775 13 69216156 9 919 15 71450806 15 83588409 19 2769165 16 65768433 15 99326324 10 79494476 13 19 14 185686257 13 2394104 14 52955627 14 49716949 14 7226775 10 69216156 14 91 13 71450806 13 83588409 14 2769165 14 65768433 12 99326324 13 79494476 15 19 12 18588257 11 2394104 11 52955627 12 497 16949 9 722877502 14 69216156 11 91 13 71450806 11 83588409 13 2769165 9 657684326 12 99326324 13 79494476 15 19 Il b pi Fa fF 1_o1 Gereed Since there is a maximum of 256 columns per sheet new sheets will be added after column 256 IV is filled The first sheet will be called F1 for history channel 1 F2 for history channel 2 etc If extra sheets are added a suffix will be added to this name e g Fl_01 F1 02 etc If the function type View Spectrum has been selected for more than one History Channel Function e g Master channel for Fl Slavel channel for F2 then the different history channel sheets named F1 and F2 are located in separate workbooks and must be saved under separate filenames This is also the case for the sheet with 8 History Channel
61. 4 2 2 Calibrate Wavelength Application Restore Original Calibration This option enables the user to restore the calibration coefficients to their original values 1 e the values that AvaSoft was shipped with If a new calibration was performed with a limited number of peaks or over a limited wavelength range the results could be less favorable This option allows the user to undo unwanted changes to the calibration 56 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 4 3 Applications Color Measurement L a B chart xt m UM see E Bn L 64 40 LRef 64 42 Channel Master a 70 36 aRef 70 38 Illuminant D65 b 50 50 bRef 50 56 Observer 2 degrees X 55 10 PID red sticker Filename C AvaSpec60 data 0208003S1 stickers txt The AvaSoft Color Application has been developed to perform on line color measurements with a spectrometer system It can be used for reflective color measurements in earlier versions of AvaSoft called color of object measurements The CIE 1976 L a b color parameters are calculated as well as other frequently used parameters like Hue Chroma and X Y Z These parameters can be displayed in a CIELAB chart or in a graph versus time It s also possible to save the measured L a b values online to a database and use one of the products from the database as a reference color By comparin
62. 440 nm Luminous Flux e 0 04290 Lumen 9 None Center WL 590 35 nm Intensity 2 1312 Peak WL 588 18 nm 10 None Intensity 2 1749 Centroid WL 592 64 nm DU 589 548 nm Intensity 2 0629 Purity 97 95 unit pUWatt AvaSoft IRRAD has been developed to perform on line absolute irradiance measurements with an Avantes spectrometer system A calibrated light source AvaLight HAL CAL or AvaLight DH CAL with known energy output in yWatt cm nm is used as a reference Also the calibration of an Avantes spectrometer system can be done in our calibration lab after which this calibration can be loaded Color of light parameters can be expressed by the chromaticity coordinated x y and z These chromaticity coordinates are obtained by taking the ratios of the tristimulus values X Y and Z to their sum The tristimulus values X Y and Z and the spectral irradiance are computed in a wavelength range from 380 nm to 780 nm using a 1 nm interval These parameters as well as the coordinates u v and the color temperature of an external light source can be calculated and displayed in real time For LED measurements the Dominant Wavelength and Purity are interesting parameters which can be calculated as well The same experimental set up spectrometer with fiber optics and cosine corrector or integrating sphere 1s used to calculate the photometric and or radiometric parameters of the light to be measured The calculated output ca
63. 48 only The pixels of the CCD detector AvaSpec 2048 are thermally sensitive which causes a small dark current even without light exposure To get an approximation of this dark current the signal of the first 14 optical black pixels of the CCD detector can be taken and subtracted from the raw scope data This will happen if the correct for dynamic dark option is enabled As these 14 pixels have the same thermal behaviour as the active pixels the correction 1s dynamic Note that this option is different from the dark current that needs to be saved before any transmittance or absorbance measurements can be taken File Save Dark If the correct for dynamic dark option has been changed it will be necessary to save a new dark and reference spectrum because the raw data has been changed If this menu option is preceded by a checkmark the scope data is corrected with the dynamic dark algorithm It is recommended to leave this setting checked which is the default state Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 25 Avantes website http www avantes com email Info avantes com antes 3 2 5 Setup Menu Subtract Saved Dark This option is used to subtract the dark spectrum that has been saved File Save Dark from the raw scope data After starting up AvaSoft this menu option is always unselected because a dark spectrum needs to be saved or loaded before it can be subtracted If this menu option is preceded by a checkmark
64. 559692 0 16 61596 0 0 0 0 D 0 22 07 03 2002 11 32 40 41560003 0 23 0465 0 0 0 0 D 0 23 07 03 2002 11 32 40 41560313 O 17 72162 0 0 0 0 D 0 24 07 03 2002 11 32 41 41560614 0 22 07574 0 0 0 0 0 0 25 07 03 2002 11 32 41 41560924 O 22 14798 0 0 0 0 D 0 26 07 03 2002 11 32 41 41561235 0 23 95353 0 0 0 0 D 0 27 07 03 2002 11 32 42 41561535 0 16 97784 0 0 0 0 D 0 26 07 03 2002 11 32 42 41561646 0 21 8668 0 0 0 0 D 0 29 07 03 2002 11 32 42 41562156 0 22 19735 0 0 0 0 D 0 30 07 03 2002 11 32 42 41562467 O 21 85271 0 0 0 0 0 0 31 07 03 2002 11 32 43 41562767 0 17 15362 0 0 0 0 D 0 32 07 03 2002 11 32 43 41563087 0 20 67904 0 0 0 0 D 0 33 07 03 2002 11 32 43 41563388 0 20 19298 0 0 0 0 D 0 34 07 03 2002 11 32 44 41563688 0 17 33064 0 0 0 0 0 0 35 07 03 2002 11 32 44 41563999 0 24 58224 D 0 0 0 D 0 ld 41 gt bi AvaSoft Data a 2 2 Sear 7 z2 Gereed The sheet will be called AvaSoft Data the columns contain the following data Column A contains a data time value formatted as dd mm yyyy hh mm ss This is a floating point value in which the integer part is the day number starting at January 1 1900 with day 1 The fractional part represents a decimal time value where 0 5 is 12 00 h noon and 0 75 is 18 00 h This way differences in date time can be readily calculated by subtracting values which would be much more difficult if this was a text representation Column B contains a time val
65. 6 2 7 23083496 3 342315674 3 006736755 3 205055237 2 803 4 814117432 1 7605896 4 470443726 0 497 169495 3 27 7122498 3 30783844 2 080 2 205491943 1 164115906 0 276916504 2 342315674 0 006736755 0 205055237 0 196 0 614117432 2 2394104 0 470443726 1 502630505 4 72207 7502 4 69216156 1 080 2 205491943 0 635884094 1 276916504 0 657684326 2 993263245 0 205055237 0 803 1 814117432 0 7605896 1 529556274 0 502830505 0 72287 7502 0 30783844 2 080 6 714508057 5 635884094 6 276916504 5 657684326 6 993263245 2 794944763 4 196 9 165882568 5 2394104 4529556274 9 497169495 6 722677502 2 69216156 6 919 7 7 14508057 9 635884094 6 276916504 4657684326 993263245 4794944763 5 196 10 16586257 5 2394104 2 52955627 4 3 497169495 8 722677502 469216156 6 919 9 714508057 5 635884094 6 276916504 5 657684326 4993263245 7794944763 9 196 7 185662568 6 2394104 529556274 9 497169495 5 72207 7502 8 69216156 8 919 7 714508057 11 63566409 5 276916504 10 65768433 993263245 6 794944763 13 19 10 16566257 14 2394104 9 529556274 7 497169495 9 72207 7502 69216156 9 919 9 714508057 13 63566409 11 2769165 10 65768433 993263245 10 79494476 10 19 185662568 10 2394104 10 52955627 11 49716949 3 722877502 10 69216156 10 91 11 71450806 7 635684094 9 276916504 6 657684326 6 993263245 6 794944763 13 19 9 165662568 6 2394104 11 52955627 9 497169495 9 722677502 10 69216156 11 91 15 71450806 17 83566409 13 2769165 15 65768433 14 99326324 10 79494476 12 19 11 16566
66. 6 2 for AvaSpec 102 256 1024 2048 Manual doc 87 Avantes website http www avantes com email Info avantes com Save ICF Load ICF buttons AvaSoft automatically saves all parameters irradiance parameters save options to the file irrtime62 1n1 and restores these parameters the next time AvaSoft is started With the Save ICF and Load ICF buttons it s also possible to save these parameter settings to and load from a file with the extension icf With this feature it is possible to save for each experiment a different icf file so it can be loaded a next time the same settings are required After the definition of one or more functions the OK button is clicked to start measuring the CANCEL button to leave the dialog and return to AvaSoft s main window After starting the irradiance measurements by clicking OK the output of the selected parameters will be displayed against time If the option Display no graphics to speed up data processing has been marked in the function entry dialog the function output will be shown by numbers only which are updated each time a new scan is saved to the output file There are six buttons at the top of this window see figure below an Exit button a Pause Start button a Save Reference button a Save Dark button an Info button and a Print button If the red Exit button is clicked the time measurements are ended and the main window and menu is activated The yellow Pause button can be used to
67. 82 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes The wavelength range is the spectral range over which the radiometric output will be integrated For example by specifying the UV C UV B and UV A wavelength ranges at three different lines the irradiance for these spectral ranges can me measured simultaneously assuming the spectrometer wavelength range includes the specified ranges and has also been calibrated for these ranges Note that the available parameters depend on the settings in the irradiance chart settings dialog For example to be able to measure the radiant intensity the Calculate Power and or Energy emitted by a v Radiometric Parameter Selecti i O x d Edit Radiometric Parameter at line 3 zi Radiometric Parameter Horne Irradiance Power received Energy crm Energy received Photon Flux ulate cro ullatt uJoule cm Joule Mol s m source option should be enabled Dhntan Flw nMan a Graphics g 14 000 a7 a 12000 ng 10 000 E O ano0 u Yaa z 5000 4 000 i 2 000 Ot x S00 550 600 B50 400 600 800 4 000 Wavelength nm Wavelength nm Expand Size Radiometric Graph Scope Data Graph Chromaticity diagram The chromaticity diagram is used to visualize the colorimetric measurements According to what has been selected in the irradiance chart settings dialog it wi
68. 9E 3 100 9 74 17 40236 15 06 30 0 92 81 79 3 756E 3 100 2 73 77 40201 15 06 30 1 07 84 69 3 209E 3 101 0 73 43 40202 15 06 30 1 22 82 19 7 877E 4 100 3 74 11 40188 54 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 4 2 Applications Wavelength Calibration Please refer to section 3 2 2 for a description about the polynomial that converts the pixelnumber on the detector into the corresponding wavelength at the X axis in AvaSoft If a Mercury Argon light source AvaLight CAL is available together with suitable optical fibers an automatic wavelength calibration can be performed The recommended optical fibers used for auto calibration are FC IRO08 2 8 um core Vis NIR 2 meters long SMA FC IRO50 2 50 um core Vis NIR 2 meters long SMA FC UV050 2 50 um core UV Vis 2 meters long SMA An important precondition for a successful auto calibration is the absence of saturation The easiest way to assure this is to select the check on saturation option section 3 2 8 1 If the spectrometer is saturated at minimum integration time a fiber with smaller core diameter e g FC IR008 2 needs to be used As an alternative the incoming light can be attenuated e g by using an attenuator FOA Inline or neutral density filter 4 2 1 Calibrate Wavelength Application Perform New Calibration The procedure to perform an auto calibration is as follows
69. A will be selected instead of D65 just as the color of an object will look different in average daylight or in incandescent light 2854K blackbody e CIE Standard Observer The original CIE standard observer functions x y and z were defined in 1931 and are known as the 2 degrees standard observer values The 2 degrees corresponds with the angle of vision that was used in the experiments to determine these standard observer values In 1964 the CIE recommended the use of different observer values for a higher correlation with visual perception for large samples These are known as the 1964 supplementary standard observer or 10 degrees observer values Both standards are still in use and in AvaSoft the CIE standard observer can be selected by clicking one of the radiobuttons The default is 2 degrees Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 59 Avantes website http www avantes com email Info avantes com antes e Display settings The measurement results can be displayed graphically in 3 charts 1 the LABChart in which a and b are shown by a moving dot in a CIELAB graph 2 the Measured Color Panel which displays the color which is being measured Note that the displayed color on the monitor may not match exactly the color of the object depends on monitor but it will give a good impression 3 The Reference Color Panel which displays the reference color that has been selected under the reference color
70. Calibration Line First Order Z Linear Default C Second Order Quadratic Actual Calibration Filename Master example cal Load Read bsorbance for point 1 Calculate New Calibration Line Calibration Points Poe pe e one 0 000 0 106 100 000 100 451 200 000 198 800 300 000 300 643 gi Calibration Line Coefficients Men E aoe maas Exit Intercept Coef 1 Coef 2 St Dev application is started Once a calibration has been saved it can be loaded later on and modified if needed Section 4 8 2 2 describes how to modify a previous saved calibration file 4 8 2 1 Start New Calibration A new calibration is started by clicking the Start New Calibration Clear Data button All data fields for the selected spectrometer channel will be cleared Enter the wavelength for the measurements in the Peak at field and the width of the peak that will be used in the Width field The program will integrate the absorbance values from Peak minus Width to Peak plus Width and uses this integral as the absorbance value in the concentration calculations M Chemometry Settings Select Variables Master k Peak at nm Width nm Conc Unit Channel Start New Calibration Clear Data Fit Order of Calibration Line k First Order Linear Default Second Order Quadratic concentration 0 Absorbance
71. First a message box appears in which the value of the color temperature in Kelvin of the light source that has been used as a reference can be entered If light from the reference light source is viewed the Planck curve for the color temperature of the reference light source is displayed The maximum of the reference Planck curve has been set to 100 3 3 5 View Menu Channel Active Channels After selecting this option a dialog is shown in which the channels to be displayed can be selected Depending on the number of available channels in the spectrometer system that is used up to 8 spectrometer channels can be selected If displaying graphs that were saved before File Display Saved Graph the active channels also remain visible to be able to measure online against a saved graph background To view only the saved graphs all active channels need to be unselected 3 3 6 View Menu Change Graph Scale After selecting this option a dialog is shown in which the range for both X and Y axis can be changed To switch to the Min Max full scale the View Graphic Reset option or the mouse zoom X Axis 600 0 630 0 out feature can be used By clicking the Save button in this dialog the settings for X axis and Y Axis will be saved to a Y Axis 48 04 324 3 file and can be restored in the future by selecting the menu option View Goto Preset Scale or by clicking the corresponding button in the button bar 3 3 7 View Menu Graphic
72. If the ProductID already exists a dialog will be shown in which the choice can be made to overwrite the old color parameters with the new ones or to keep the old values for this productID As an alternative a reference color can be added to the database during the online measurements This can be done only if in the LABChart settings dialog the option Add new reference colors to DB when measuring is enabled Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 61 Avantes website http www avantes com email Info avantes com avantes After all settings are filled in correctly the Lease measurements can be started by clicking the OK button or using the Enter key on the keyboard A shortcut to start measuring with LABChart directly omitting the settings dialog is clicking the n LABChart button in AvaSoft s main window The figure at the right shows all color parameters and all 3 charts were enabled The measurements in h xt Im UM see El L 64 40 the figure are performed with the Master a Se a 70 36 Illuminant D65 b 50 50 bRef 50 56 spectrometer channel and illuminant D65 has been nserver 2 aegrees x 55 10 PID red sticker 33 30 aL 0 02 selected The color parameters are calculated with z 9 31 da 0 02 the 2 degrees CIE standard observer values The ae ose oe filename to which data will be saved can be found in the lower left corner Filename C AvaSpec60 dat
73. ProductID red sticker J Add New Reference Colors to DB when measuring i X Cancel The color measurements in the LABChart are started by clicking the OK button in the figure above The following parameters can be set before the measurements are started e Spectrometer Channel If the spectrometer system contains one or more slave spectrometer channels the spectrometer channel on which the color will be measured can be selected from the drop down box at the upper left corner showing by default Master Make sure white reference and dark spectra have been saved before for the selected spectrometer channel e Illuminant The CIE Standard sources A B or C or one of the illuminants D50 D55 D65 or D75 can be selected by clicking one of the radio buttons The default is D65 which is the most widely used illuminant It represents the power distribution of average daylight with a correlated color temperature of 6500K The D50 D55 and D75 have correlated color temperatures of 5000K 5500K and 7500K The CIE Standard sources A B or C represent respectively Incandescent light 2854K blackbody Simulated noon sunlight and Simulated overcast sky daylight Note that there is no relation between the illuminant chosen here and the light source that is used for measuring the color of an object The selected illuminant is used in calculation of the color parameters These color parameters will be different if for example
74. TL switch at the lightsource needs to be in TTL position In AvaSoft the menu option save automatic dark by TTL shutter needs to be enabled in the menu setup options If this option is enabled the TTL will close the shutter of the light source at the moment the dark data is saved After the dark has been saved the shutter will be opened automatically 3 2 8 7 Setup Menu Options External Trigger Setting The DB15 connector on the WgeExternal Trigger Setting E 5 x AvasSpec spectrometer uses two pins for digital IN communication pin 4 and 8 C Perform scans continuously save first available spectrum if TTL signal is high pin 8 Pin 4 is used for the Hardware Perform scans continuously save first available spectrum as Reference if TTL signal is high pin 8 Trigger mode and pin Sisa External Hardware Trigger perform a single scan at rising edge of TTL pulse pin 4 regular digital in which status can be polled In AvasSoft two W Automatically save on Hardware Trigger functions have been defined for using the digital in at pin 8 A lot _X Cancel more customer specific features can be easily implemented For generating a 5V signal at pin 8 manually the handheld pushbutton cable IC Extrig 2 may be ordered or if the Automatic Save Dark by TTL shutter option needs to be supported as well an IC DB15 Extrig 2 Y cable can be ordered The different options which are shown in the dialog above are describe
75. Table Filename l D Mtest data le pet displayed as shown in the figure below DAtest data example pet To select one of the products from the list click on one of the numbers 1 11 in the first gray ProductD LREF aREF b REF Color column 2 19280 2720 31 840 To delete a product from the list select it and Ha 370 ogo 36450 click the delete key on the keyboard 43 240 37 950 48 060 To search for a certain productID or number 42290 44430 8990 click the Find button 59 740 22910 34610 oo a a EI i To sort the records by ProductID L REF a REF B2240 7570 72130 E E a R r1 r40 26 650 63 440 50 110 59 160 45 770 J 632 8 nm 52 710 61 310 43 720 or b REF click on the corresponding column header A yellow marked 1 will be shown in the column header By clicking again at the 10 570m o ta ace header which is already marked the sorting 11 694 nm za ean o direction will be converted By clicking the Cancel button the selected product will not be moved to the current a ieee reference color in the LABChart settings dialog 4 e and changes e g deleting a product from the list will be ignored By clicking the OK button the current reference color in the LABChart settings dialog is changed to the color which has been selected To add the current reference color specified in the LABChart settings dialog to the database file the Add button below of the select button can be clicked
76. VASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 7 Avantes website http www avantes com email Info avantes com antes 1 Quick Start Measuring and saving a spectrum 1 After starting AvaSoft the green Start button needs to be clicked to start measuring Connect a fiber or probe to the light source and to the spectrometer input port s and set up the experiment for taking a reference spectrum 3 Adjust the Smoothing Parameters in the Setup menu section 3 2 3 to optimize smoothing for the Fiber Slit diameter that is used 4 Now turn on the light source Usually some sort of spectrum may be seen on the screen but it is possible that too much or too little light reaches the spectrometer at the present data collection settings Too much light means that over a certain wavelength range the signal is saturated shown as a straight line at the maximum counts and the appearance of the label saturated in the statusbar of the spectrometer channel This can usually be solved by a shorter integration time The integration time can be changed in the main window in the white box below the start stop button If AvaSoft is collecting data the start stop button shows a red stop and the integration time box is gray indicating that it cannot be changed After clicking the stop button the data acquisition stops and the integration time can be changed The result of the changed integration time can be viewed after clicking the green
77. Visualize saturated pixels areas in graph Log saturated pixel areas to screen Log saturated pixel areas to file on Save Measurement Visualize saturated pixels areas in graph By enabling this option the spectrum in the main window will be displayed with a thicker line for these wavelength ranges where the saturation occurs In time series measurements the function output values that were calculated using saturated data are marked with a black dot Pe Ae atadt OT fed te beaters 6 O31 DRE eR ove z 6 s 205 FERTUTEN E E ifj i m BRS SATIOR G Ab i m miegui tee pret fy wem i rew e pa Mete ike ATODOL TR Angio POA Togi he CFF Sed Log saturated pixel areas to screen By enabling the second option a small window appears in which the spectrometer VEE ox channel number 0 Master 1 Slavel etc and wavelength range for the saturated ae Chan From To nm pixels are given 0 47352 751 53 i T5222 foece E Log saturated pixel areas to file on Save Measurement The third option can be used to create a file to which the saturated wavelength ranges will be written when a measurement is saved in AvaSoft When saving spectra in the main window Save button or menu option File Save Experiment the name of the logfile becomes equal to the experimentname but with the extension sat For example if a graph in absorbance mode is saved to test0001 abs and 1f this spectrum contains saturated data
78. a 0208003S51 stickers txt The button bar in the LABChart shows the following buttons 62 The Exit button is clicked to stop the color measurements and return to AvaSoft s main window The white and dark reference buttons have the same functionality as in AvaSoft s main window The Save as reference color button is used to update the current reference color After clicking this button a dialog is displayed in which a name for 7s ial ES the ProductID needs to be entered If the productID already exists in the database file and The praductiD yellow already exists if the option Add new reference colors to DB The curent color for this productiD in the database iz when measuring is enabled in the LABChart ters 9642 aner 1068 preps 7265 settings dialog then a dialog will be shown in which the choice can be made to overwrite the old color parameters with the new ones or to keep the old values for this productID see figure tere sea aera 1071 bree 72 86 at the right The select button can be used to select a new reference color from the database as described in detail on the previous page Each time the Save button 1s clicked a new record is added to the Filename in the lower left corner One record contains the following fields Date Time Comments L a b h c X Y Z RefProductID Lref aRef bRef dL da db dE Channel Illuminant and Observer The Comments field contains the text that
79. alled LABChart and shows the input parameters for displaying the color measurements in a color chart the other one is called Time Series and shows all input parameters for displaying up to eight color parameters in a graph versus time The input parameters will be described in detail in sections 4 3 2 1 LABChart and 4 3 2 2 Time Series The color measurements are started by clicking the OK button AvaSoft returns to the main window if the Cancel button is clicked 58 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes v Color of Object Settings S 5 x LABChart Time Series 4 3 2 1 LABChart 5 a TAPE Mllluminant CIE Standard Observer pectrometer Lhannet ITA amp C D55 C D75 2 degrees Master CB C D50 D65 10 degrees Display Settings Display following Color Parameters J Display LABChart Iv L Jv x MH M dL J Display Reference Color Panel lv a Iv Y Iv C v da MV Display Measured Color Panel Iv b lv zZ Jv dE J db Save Settings Save experimental results to file C AvaSpec60 data 020800351 stickers txt Change Output File Reference Color ca DB File D Source S W6_Delphi6 example pct Current Reference Color Select Select Current Reference Color from Table LREF 64 42 aREF 70 38 bREF 50 56 Add Add Current Reference Color to Table
80. amed Fx_script txt where x is the history channel number from to 8 A starter script has the following contents Function F2 value F2 0 End Function You can elaborate on this function as long as there is a value assigned to F2 in the end In this case F2 will be assigned the value 0 You can refer to the other history channels with the predefined variables F1 to F8 If you want to use data from the spectrometer you will need to assign other history channels to e g wavelength values or integrals The following script defines the function value for F3 as the quotient of F1 and F2 Function F3 value F3 F1 F2 End Function Please do not refer to the function value for the script itself You can also not refer to other functions if these are themselves assigned to a script This will result in a scripting run time error If you want to use code from another script please copy the necessary lines from that script into the one your working with Please understand that a script is executed by an interpreter on a line by line basis If you make a syntax error in your script it will usually only show at the moment the line with the error is executed as a run time error Nothing will stop you for instance from entering the following script for function 3 F3 F1 0 x End Function 11 Microsoft VBScript runtime error Division by zero at line z char l near Running this script will result in the run time error a
81. andard illuminant the reflectance R or the transmittance of the object and the CIE standard observer functions x y and z 2 degrees or 10 degrees angle The integral of these products over all the wavelengths in the visible spectrum 380 to 780 nm with a 5 nm interval gives the tristimulus values Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 57 Avantes website http www avantes com email Info avantes com antes The chromaticity coordinates x y and z are obtained by taking the ratios of the tristimulus values X Y and Z to their sum X Y vA X gt y SO ZL gt X Y Z X Y Z X Y Z Another well known way to present the color parameters a and b is by their hue angle h and Chroma C Hue angle is measured in degrees starting with h 0 in the red direction a and increasing counterclockwise h arctan a Chroma is defined as the length of the line from the point a b 0 to the sample point Ceara To describe color differences a well known parameter is AE 4 which is defined as AE pa 4 AL Aa Ab In which AL Aa and Ab represent the difference in L a b values between the reference color and the actual measured L a b values 4 3 2 Menu option Color Measurement After selecting the menu option Applications Color Measurement a dialog is displayed in which the input parameters can be set The dialog shows two tabbed pages one is c
82. are shown If the calibration has been performed with diffuser the intensity calibration data will be saved to an ASCII file with extension dfr with bare fiber this extension will be fbr The name of the intensity calibration file can be entered after clicking the Save As button Switch to the Irradiance Chart TAB to enter the hardware setup and select the colorimetric radiometric photometric and or peak parameters of interest see section 4 4 5 1 Then click OK Measure the output parameters in the experiment If needed change the integration time such that the maximum in Scope Mode is around 14000 A D Counts Block the light path to the spectrometer and save a dark spectrum If the ir radiance of the light to be measured needs to be displayed against time click the time measurement TAB in the settings dialog as described in section 4 4 6 The intensity calibration as performed under point 9 can be loaded in future experiments by selecting the option Load Intensity Calibration as described below under Quick Start 2 After loading an intensity calibration a dark spectrum needs to be saved before switching to Irradiance mode Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 75 Avantes website http www avantes com email Info avantes com antes Quick Start 2 Perform absolute irradiance measurements by loading an intensity calibration 1 Start the AvaSoft software and click the Start button in the ma
83. asoft Oxygen has been developed to perform on line absolute oxygen measurements with a spectrometer system Oxygen probes are based on the quenching effect of oxygen on the fluorescence of a ruthenium complex The AvaLight LED 475 light source used emits light with a wavelength of approximately 475 nm Measurements of the fluorescence are performed at approximately 600 nm A high signal corresponds with a low oxygen level and vice versa The calculated oxygen level can be displayed and saved in two ways e The oxygen level can be displayed on line in a separate display window with up to eight channels e You can select up to eight history channels that can display and save oxygen levels against time This application can be combined with the Excel and Process Control applications 4 7 Oxygen application 4 7 1 Quick Start How to take oxygen measurements with AvaSoft Oxy 1 Start the AvaSoft software and click the Start button in the main window Connect one leg of the bifurcated fiber optic cable to the input of your spectrometer and the other leg to your blue LED lightsource Use an SMA interconnect ME FI SM MM to connect the oxygen probe to the common part of the bifurcated cable 3 Set the Smoothing Parameters in the setup menu Setup Smoothing and Spline to 10 4 Before oxygen measurements can be performed the oxygen probe must be calibrated at two known levels of oxygen such as 0 0 percent oxygen and 100 0 percent
84. at the right A number of peak parameters can be calculated from this spectrum j 500 550 600 650 700 Wavelength nm FWHM and Center Wavelength The Full Width Half Maximum of a peak is the bandwidth in nanometers for which the intensity is higher than half of the maximum intensity of that peak feces screens The Center Wavelength is the wavelength halfway between the left and right om og a wavelength where the intensity is half of the maximum intensity FWHM Vac ial beak nr Peak Wavelength a occa ae Wavelength at the maximum spectral power Peak WL 566 30 nm Intensity 2 3755 CS id WL 570 79 nrm Centroid Wavelength a total spectral power left and right from the centroid wavelength integral is gt unit puwatt the same 70 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes The radiometric parameters can be grouped into three categories Radiant Flux uWatt The radiant flux is the total optical power emitted from a source in all directions The best way to measure the power emitted by a source is to measure the source inside an integrating sphere This is often done when measuring LED s It is also possible to calculate the flux of a source by measuring the irradiance at the surface of the diffuser cosine corrector or integrating sphere sample port at a certain distance from a light source An important assumption in
85. atic mode the reference data that will be loaded are shown on the screen first 3 1 4 File Menu Load Experiment With this option an experiment can be loaded that has been used before This way more spectra can be saved to an existing gs History Load Experiment Name experiment An experiment name has the file extension kon After choosing this option a dialog box shows all experiments that were saved earlier in the current experiment directory If the ILA experiment name that needs My ae to be loaded is in this directory select it and click es ene flaser kon ay the save button If the My Network F g Files of type Experiment Files experiment name that needs to be loaded is in another Cancel me drive and or directory move to this directory by clicking the z behind the current foldername For detailed information on graphic filenames see File Save Experiment 3 1 5 File Menu Save Dark With this option dark data are saved The name of the dark data file is dark dat where represents the number of the slave channel for which the dark data has been saved 0 represents the master channel The dark data files will be saved in the experiment directory that has been picked by the option File Load Experiment or File Start New Experiment 3 1 6 File Menu Save Reference With this option reference data are saved The name of the reference data file is ref dat where re
86. avantes com email Info avantes com antes 4 4 5 Irradiance Chart 4 4 5 1 Irradiance Chart Settings Calibration Inadiance Chart rradiance Time Spectrometer Channe After an intensity calibration has been performed or loaded the Irradiance Master E Chart TAB becomes available in the Irradiance Settings dialog By clicking the TAB the Irradiance Chart settings are displayed The figure at the right shows the default settings for Spectrometer Channel Hardware Setup Parameter group Graphics and Save Settings Hardware setup Detector type f COU VIS or Fiber f AvaSphere Parameter group The Spectrometer Channel setting can be changed if for a multichannel spectrometer system calibrations have been performed or loaded for more than one channel I Colorinnetry The Hardware setup setting is an important parameter because as described m landat in section 4 4 1 background it determines the radiometric and photometric parameters that can be measured as well as the way these are calculated If TEE an AvaSphere is used it is important to know 1f the light source is F Seva E ey measured inside the sphere like often is done with LED s or outside the M Display raw A D Counts Graph sphere This setting can be entered after clicking the AvaSphere button and E Diles medene Esp then inside or outside sphere Peak Measurement Save Settings Save experment results to file The Parameter group setting
87. ave Reference for FOS reference channel oy 10 000 5 3 000 FOS correction mode soon Software user controls FOS switch frequency F 7 ood f Auto FOS switch frequency is preset f Continuously measure sample update FOS reference on switch Continuously update FOS reference measure sample on switch Update FOS reference every Eo seconds FOS Reference Sample Reference XM Cancel 400 500 B00 T 800 900 Wavelength nm If the interface cable between AvaSpec and FOS 2 and the Power Supply are properly connected the FOS 2 will close top sample channel and open the bottom reference channel As long as the FOS settings dialog is not closed the FOS 2 remains in this position If you want to use the second method to correct for drift by saving a new reference see previous page click the checkbox FOS Reference Sample Reference at the bottom of the dialog When using the first correction method the integration time for the FOS reference channel can be set to a different value as for the FOS sample channel Remember to save a new dark and reference for the FOS reference channel after changing the integration time Determine a good integration time and save a FOS reference by clicking the white button Then switch off the light source and save a FOS dark spectrum by clicking the black button The FOS correction mode as shown in the FOS settings dialog can be set to Software or to Auto
88. ave settings Output parameters are saved to imchart2 tt Save radiance spectrum ves By clicking the save dark black button a new dark spectrum will be saved It is recommended to save a new dark spectrum each time after changing the integration time icki Bi 7 Clicking the save data button File Edit Format Help will add the measured data to IRRADIANCE MEASUREMENT RESULTS the textfile A user comment Spectrometer 021200652 Channel Master 13 8 2004 14 58 21 DetectorType AvasSphere source inside sphere can be added to the saved data Diameter Sampleport AvaSpere 10000 micron Smoothing parameter 6 If a radiometric spectrum has Irradiance Spectrum saved to D source sw6_Delphi6 data 0212006S2 IRRCHART20004 IRR User comments yellow led S N 030812344 jr been saved with the output Meee oo WHR Hee Colorimetry Dw 572 276nm parameters the name of this Purity 97 44 file is also saved in the textfile f 97337 HHHHHH HH Phot omet ry Luminous Flux Ce 0 05369 Lumen Power e from 380 00 to 780 00 nm 94 057 pwatt www Doak Measurements from 380 00 to 780 00 nm Full width Half max 31 421 nm Center wavelength 568 36 nm Intensity at CwL 2 3515 pwatt Peak wavelength 566 30 nm Intensity at Peak 2 3742 watt centroid wavelength 570 40 nm Intensity at Centroid 2 2677 watt The Display Saved Graph button can be clicked to load a radiometric spectrum that was saved before I
89. cking the Pause button data acquisition stops and the text on the button changes to a green Start If the Start button is clicked data acquisition is activated again and the text changes back to the yellow Pause again The Save Reference and History Channa Functions Exit Into GS Save Dark buttons have the same functionality as in the i i la nae i A Tice e dis y A Y main window on DR yon ji hgh fia inti f aan FAL i My W it dhe t Tan T i p Yal 1 r 5 F L i The Info button shows the ups Function Entry dialog in which all parameters can be viewed not edited while the measurement is running By clicking the Print button the graphics that are displayed can be printed during the run First the printer specific dialog shows up in which the print options can be set For a _ example if only one graph of the four in the figure above need to be printed this graph can be selected by the page s radiobutton All graphics are printed full size on a different page Zoom features In each graph the same zoom features apply as in the main window except for scaling the Y axis with the mouse wheel However zooming in over the X axis while the measurement is running and the X axis is already scrolling will not be possible because in that case the X axis 1s updated with each new scan Clicking the pause button to take a snapshot will solve this problem Zoom in select a region to be expanded to the full gra
90. d below Perform scans continuously save first available spectrum if TTL signal is high pin 8 In this mode AvaSoft will poll the status of pin 8 after each arriving scan If the status of pin 8 is HIGH 5V the spectrum or spectra in case more spectrometer channels are active will be saved automatically as described in section 3 1 7 A comment dialog will not be shown instead the spectrometer channel number and a time stamp will be written to the comment file Note that the spectrum will be saved only if the signal is high at the moment of polling which is the moment that AvaSoft receives a new spectrum from the data acquisition driver If a short TTL pulse is generated at pin 8 there is a big chance that the 36 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes spectrum is not saved To save one spectrum the duration of the TTL signal should therefore be as long as the product of integration time and number of scans to average In time critical situations it is recommended to use the External Hardware Trigger Mode Perform scans continuously save first available spectrum as Reference if TTL signal is high pin 8 For absorbance or reflectance transmittance measurements it is recommended to update the reference data regularly to exclude effects like drift in the light source or e g temperature effects of the detector or in the optical bench I
91. e also section 3 2 8 1 check on saturation Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 13 Avantes website http www avantes com email Info avantes com antes 2 6 Find peaks or valleys by CTRL or SHIFT left mouse button click This option can be used in all modes Scope Absorbance Transmittance or Irradiance and for all displayed graphics When the left mouse button is clicked in the graphical region while the CTRL key is down AvaSoft will follow the following procedure to run to the closest peak 1 The wavelength is determined from the position the mouse click occurred The data from closest pixel is retrieved 3 The direction to search for the peak is determined from the neighbor pixels If both neighbor pixels have a lower value at the Y axis than the current pixel the current pixel is already a peak If only one of the neighbor pixel values is higher then the current pixel value the peak will be searched in the direction of this higher pixel If both neighbor pixels have a higher value at the Y axis than the current pixel the current pixel is in a valley The peak will in this case be searched in the direction of this neighbor pixel with the highest value 4 The cursor starts moving in the direction as determined under 3 until it reaches a pixel of which the value is not higher than the last one evaluated At this pixel the cursor stops By holding down the SHIFT key instead of the CTRL key the same pr
92. e list Displaying HISTORY DAT Measurements starte d at 02 07 01 Time 10 20 10 _Exit Pause inf 2 The zoom features as described in section 4 1 2 can be applied to zoom in on an interesting time interval A description for the print button can also be found in section 4 1 2 Since the History Channels Output file is in ASCII this file can be easily imported in a spreadsheet program like Microsoft Excel The data in the file can also be viewed with a text editor like Microsoft Word or with Notepad An example of the file format is given on the next page Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 53 Avantes website http www avantes com email Info avantes com antes History Channel Functions Measurement Report Measurements started at 29 06 01 Time 15 06 29 Integration time 3 ms Average scans Description of the Functions FUNCTION 1 User Defined Function m 400 Scope Mode FUNCTION 2 User Defined Function m 500 Absorbance Mode FUNCTION 3 User Defined Function m 600 Transmittance Mode FUNCTION 4 User Defined Function m 700 Irradiance Mode FUNCTION 5 Integral Master Channel between 380 0 and 780 0 nm Multiplied with 1 000 Transmittance Mode FUNCTION 6 View Spectrum in Transmittance Mode No Data Saved FUNCTION 7 Not Activated FUNCTION 8 Not Activated Time Seconds Fl F2 F3 F4 F5 15 06 29 0 62 79 79 2 294E 3 100 8 74 11 40192 15 06 30 0 77 81 84 2 05
93. e mesumsmemm memammos Snoseuo Sare rF to use the customized reference data in AvaSoft The status of this option is shown in the button bar by the addition of a green border to the white button If the border is absent 100 reflectance will be assumed for the reference tile If the border is present like in the figure above customized reflectance values will be used for all calculations 40 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 3 2 8 10Setup Menu Options Suppress Save Comments This option if preceded by a checkmark disables the appearance of the comments dialog box if an experiment is saved as described in section 3 1 7 By default this option is OFF After clicking the menu option it will be enabled preceded by a checkmark 3 2 8 11Setup Menu Options View Reflectance instead of Transmittance Although mathematically equivalent the experimental setup for a measurement in reflectance or transmittance mode is different and therefore the possibility to select the name Reflectance instead of Transmittance is implemented By default the option is not preceded by a checkmark meaning the program will use Transmittance mode By clicking the menu option a checkmark will appear and the program will use the name Reflectance instead of Transmittance The effect of the change 1s twofold e In Reflectance Transmittance mode
94. e to point out the row in the Fit Order of Calibration Line table for which sample the C Second Order 7 Quadratic ee Intercept 1 4 1 0 Coef 1 SSS absorbance value will be measured _ 2 Actual Calibration Filename Master 30 Coef 2 Say When moving through the rows in Load a bw Se the table the text in the button Read Absorbance for point x will ___ Read Absorbance forpoint be updated for the sample that will Calculate New Calibration Line be measured In the example we start with measuring the absorbance of point 1 which is the reference sample Click the Read Absorbance for point 1 button to import the measured absorbance into the left column in the table If the reference is measured the value should be very close to 0 000 The measured absorbance and corresponding concentration will be visualized as a new point in the graph Now move the cursor in the table to the second row setup the experiment for measuring the sample of known concentration for point 2 30 mmol l in the example above and click the Read Absorbance for point 1 button to import the measured absorbance into the left column in the table for point 2 Repeat these steps for the other samples of known concentration For a linear calibration line at least two samples are needed but the more points added the more reliable the calibration line will be 95 After the samples have been measured click the Calculate N
95. educe the noise during the intensity calibration 78 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes If a good reference signal is displayed click the white Save Reference button in the dialog above A white line will mark the reference spectrum Then switch off the calibration lamp wait until the spectrum becomes flat near the bottom of the scale and click the black button to save a dark spectrum A black line will mark the dark spectrum 4 4 4 2 Save Intensity Calibration v ERE Intensity Calibration a Ioj x If reference and dark data have been saved in the figure above the intensity calibration Kpg m Calibration light source used in this calibration can be saved by clicking the Save Senne NE Calibration button Select fiberoptic setup used in this calibration A dialog shows up in which the current settings in this intensity calibration are shown If the calibration has been performed with diffuser the intensity Fiber Diffuser diameter S arnple Port ISP fioo calibration data will be saved to an ASCII file with extension dfr with bare fiber Parameters in this intensity calibration this extension will be fbr The name of Spectrometer channel Master lntegrationtine f milliseconds the intensity calibration file can be entered Smoothing E piels after clicking the Save As button P ave Ag The
96. egration time The result is the amount of energy that has been emitted or received during one integration time cycle Another radiometric parameter that can be calculated from the irradiance spectrum is the number of photons that is received by a surface Since the number of photons per nanometer is a huge number even with very low light intensity the number of Avogadro is used to express the number of photons in mols or as in our application in umols The number of photons per nanometer can be calculated from the wavelength dependent photon energy and the absolute light energy that is measured A detailed description how this is done can be found at the next page The photon count distribution WMol s m nm shows the photon flux received per square meter Other photon count units that can be calculated from this are uMol s nm photon flux received at diffuser surface uMol m nm photons received per square meter during one integration cycle uMol nm photons received at diffuser surface during one integration cycle Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 71 Avantes website http www avantes com email Info avantes com ayvantes How to convert a power distribution uWatt cm nm into a photon count distribution uMol s m nm Photon energy E A h c A e Where h Planck s constant 6 626 068 76 x10 c velocity of light 2 998 x10 m s wavelength in meters For example
97. ent Parameters Display no graphics to speed up data processing Below the TAB sheets for function definition an option can be enabled or disabled to speed up data processing by not displaying the graphics during the measurements If an application requires fast data processing this option should be enabled If saved to an output file the data can be displayed graphically after the time series experiment has been ended as described in section 4 1 3 History Display Saved History Graph Function Independent Parameters Save Function Output The results of a time series color experiment will be saved to an ASCII file if the radio button Save Function Output has been selected A number of seconds between saving can be entered for data reduction in case measurements are carried out over long periods Entering a value of zero results in saving every scan The name of the file to which the data will be saved can be changed after clicking the Change Output File button Last option in the Save Function Output box is to enable or disable the possibility to create a backup file during the measurements If this option is enabled AvaSoft will create a backup file with the same filename but with the extension bak also in ASCII This backup file is updated every scan and can be used in case the filename that has been selected has failed to save the data for instance because of a power failure during the measurements Aug 04 AVASOFT
98. er and positioning integration time dark settings light source intensity ambient light temperature oxygen standard values used etc Therefore it is highly recommended to calibrate as often as possible Choose the standards in the range of your measurements and try to make the difference in signal over this range as large as possible Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 101 Avantes website http www avantes com email Info avantes com antes AvaSoft Chem was developed to enable on line concentration measurements with a spectrometer system According to Lambert Beer s law there is a linear relationship between absorbance and concentration 4 8 Chemometry A g c Where A is the absorbance or extinction is the extinction coefficient of the compound to be measured c is the concentration and is the optical path length In practice this relationship is only linear at reasonably low absorbance levels say less than 2 The wavelength at which the absorbance is measured must of course be kept constant To take concentration measurements you will need a spectrometer with either a cuvette holder or a dip probe and a suitable light source If you use cuvettes remember that glass cuvettes will absorb UV light If your wavelength is in the UV range use quartz or polystyrene ones instead AvaSoft Chem can display and save the calculated concentration in two ways The concentration can be
99. ers and the way these are calculated depend on the hardware setup The figure above shows the settings that are available when measuring with a cosine corrector or with an integrating sphere at a certain distance from the light source For each function F1 F8 one of the 35 available parameters can be selected See section 4 4 1 for background information about these parameters The colorimetric parameters can be calculated by using the 2 degrees standard observer values or the 10 degrees If one of the radiometric parameters is selected the wavelength range needs to be specified The wavelength range is the spectral range over which 86 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes the radiometric output will be integrated If the source is not inside an integrating sphere the radiant intensity in u Watts sr and radiant energy in uJoule sr can be calculated if the distance from source to diffuser surface has been specified The radiant flux uWatts emitted and emitted energy uJoule e can be calculated if the geometry of the lamp in steradians is specified Distance and Geometry can be entered if the parameter is selected for which these data entries are relevant If one of the peak parameters is selected the wavelength range needs to be entered over which the highest peak will be calculated Moreover the radiometric parameter should be specified to dete
100. ew cern 1D1 x Calibration Line button As a Vises 7 7 p Channel aster result a red line will be fit through B Mester eak at nm the measured data and you will be Width 10 nm Actual Points Actual Line a New Points New Line asked to save the calibration or not Conc Unit fm The figure at the right shows that the calibration line fits very well Corim o4 o6 og _ Absorbance through the measured points so we Qo Used 1 Calculated New Calibration Line Coefficients are pints Calibration Line Coefficients a hi conten aca tO Intercept 7 9811E 001 Abs Conc Cone Intercept save the calibration ao 9 8768E 001 0 001 0 Coef 1 E a icient 2 0 0000E 000 i If during the measurements an es 29 20 coet o St Deviation 1 07538 incorrect calibration point was ee 70 StDev Do you want to save the new calibration line measured for example because of mixing up the samples or measuring the concentration at the wrong row this will become clear from the graph You can then select either directly the No button or select the Yes button and modify the calibration line later on alculate New Lalibration Line 104 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com Mantes After clicking Yes a dialog is shown in which the name
101. f the following spectrometer types Avaspec 102 or AvaSpec 102 y Avaspec 256 or AvaSpec 256 y Avaspec 1024 or AvaSpec 1024 y Avaspec 2048 or AvaSpec 2048 y Avaspec 2048FT or AvaSpec 2048FT y In which y represents the number of spectrometer channels This manual is about the AvaSpec 102 256 1024 2048 group of spectrometers Installation Dialogs The setup program will check the system configuration of the computer If no problems are detected the first dialog is the Welcome dialog with some general information In the next dialog the destination 2 Choose Destination Location directory for the AvaSoft software can ry Setup will install AvaSpec 1027 25671 024 2045 in the following be changed The default destination folder directory is C AVASPEC62 If you To install into a different folder click Browse and select want to install the software to a another folder different directory click the Browse You can choose not to install AvaSpec 102 256 1024 2048 button select a new directory and click by cieka E anic el te eat aaup OK If the specified directory does not exist it will be created In the next dialog the name for the program manager group can be changed The default name for this is AVANTES Software After this the Start Installation dialog is shown After clicking the next button the installation program starts installing files Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Ma
102. features can be set separately for all functions F1 to F8 e Spectrometer Channel If the spectrometer system contains one or more slave spectrometer channels the spectrometer channel on which the color will be measured can be selected from the drop down box at the upper left corner showing by default Master Make sure white reference and dark spectra have been saved before for the selected spectrometer channel e Illuminant The CIE Standard sources A B or C or one of the illuminants D50 D55 D65 or D75 can be selected by clicking one of the radio buttons The default is D65 which is the most widely used illuminant It represents the power distribution of average daylight with a correlated color temperature of 6500K The D50 D55 and D75 have correlated color temperatures of 5000K 5500K and 7500K The CIE Standard sources A B or C represent respectively Incandescent light 2854K blackbody Simulated noon sunlight and Simulated overcast sky daylight Note that there is no relation between the illuminant chosen here and the light source that is used for measuring the color of an object The selected illuminant is used in calculation of the color parameters These color parameters will be different if for example A will be selected instead of D65 just as the color of an object will look different in average daylight or in incandescent light 2854K blackbody e CIE Standard Observer The original CIE standard observer functions x y a
103. following data will be saved to the intensity calibration file Name of light source calibration file lmp e Spectrometer channel O Master 1 Slavel etc Integration time in milliseconds e Diameter of diffuser surface in microns TOTAL PIXELS 2048 for AvaSpec 2048 values which represent the dynamic range reference minus dark data at each pixel during the intensity calibration divided by the intensity of the calibrated light source that was used Setting for the Smoothing parameter during the intensity calibration After an intensity calibration has been performed a graph is displayed which shows the data transfer function for the spectrometer channel The irradiance spectrum is calculated by multiplying the measured scope data from which a saved dark spectrum is subtracted with this data transfer function DO 0O oO wo Master Transfer Function uV icm counts If the intensity calibration has been performed the Irradiance Chart and Irradiance Time Series tabs 001 will be enabled Clicking one of these tabs allows you i TR a FF a a aT aT to change the settings for the irradiance chart or time pesca in series measurements after which the measurements can be started by clicking the OK button The irradiance chart and time series settings will be described in sections 4 4 5 and 4 4 6 Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 79 Avantes website http www
104. fy the user if a measurement contains saturated pixels This notification is done in such a way that the user can always decide to ignore the saturation for example if the saturation happens at pixels that are not in the wavelength range where the user is interested in Saturation can usually be solved by selecting a shorter integration time When at minimum integration the signal is still too high an attenuator a neutral density filter or fibers with a smaller diameter may be used In AvaSoft different levels of saturation detection can be set and there are also different options for notification as shown in the figure at the right v Saturation Detection a _ O x Saturation detection levels Set Saturation Detection The default level of saturation detection is Detect Saturated Pixels at saturation level Only for AvaSpec 2048 spectrometers the third Detect all Saturated Pixels and autocorrect inverted pixel values y p p autocorrect inverted pixels level is available The reason for this is that if the detector type in If one or more pitels of a spectrometer channel are saturated the text label the AvaSpec 2048 Sony ILX554 is heavily saturated appears in the QvaSott main window statusbar for that channel saturated at a light intensity of approximately 5 Additionally more detailed information can be retrieved in the following ways g y of app y times the intensity at which saturation starts it e oiaied
105. g in over the X axis while the measurement is running and the X axis is already scrolling will not be possible because in that case the X axis is updated with each new scan Clicking the pause button to take a snapshot will solve this problem 88 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Zoom in select a region to be expanded to the full graph To select this region click the left mouse button in the white graphics region and drag it downwards and to the right After releasing the left mouse button both the X and Y axis will be rescaled to the new values of the selected region Zoom out drag with the left mouse button within the white graphics region but in stead of dragging the mouse downwards and to the right drag it into another direction After releasing the mouse button both the X and Y axis will be reset to their default values Move X Y dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 89 Avantes website http www avantes com email Info avantes com antes 4 5 Applications Process Control Application Avasoft Process Control allows you to operate digital outputs corresponding with preset levels of your history channels You can use all eight history channels as well as the output functions of the Time Series f
106. g the measured L a b values to the stored database values color differences AE av AL Aa or Ab can be measured as well Emissive color measurements can be done to measure the color of a light source e g LED s These measurements require an irradiance spectrum to calculate the color parameters x y and z With AvaSoft s Irradiance application it is possible to perform irradiance measurements with a lot higher accuracy than when using the relative irradiance mode which assumes a perfect blackbody light source with known color temperature For this reason the emissive color or color of light calculation is one of the features in the Irradiance Application 4 3 1 Color of an Object Background The color of an object can be expressed by the CIE 1976 L a b color space L describes the lightness of the color A positive value of a describes the redness of the color a negative a the greenness Similarly yellowness or blueness is expressed by coordinate b which is positive for yellow and negative for blue The L a b values are derived from the CIE tristimulus values X Y and Z of the sample object and the standard illuminant tristimulus values X Y and Z The standard illuminant tristimulus values for Xa Yn and Z are constant and depend only on the type of standard illuminant that has been chosen The CIE tristimulus values X Y and Z of the color of an object are obtained by multiplying the relative power P of a st
107. gger pulse input Therefore the Start Stop button will be disabled The edit bar will show the current setting for the delay 1000 ns in the figure below Wg AvaSoft 6 1 Full 2003 Avantes S N 031199952 le j x Za Cani Ua Casaid a O OOOO OOO Ee ees i sat BRS 8 SATI O Bk wb B E Integration time ms a Average f Wavelength nm 656 87 V Trigger Delay 1000 ns LO MWe MW D P Trigger checkbox As long as the spectrometer is in this mode you cannot send commands from AvaSoft to the spectrometer So if you need to change the delay you will first need to disable the external trigger mode This can be done in the external trigger mode dialog by selecting the None button but a shortcut with the same effect is to click the Trigger checkbox in the edit bar of the main window Note that one more trigger pulse is needed before the external trigger mode is disabled The reason for this is that the spectrometer can only react on an external trigger at pin 4 before it can respond to the disable external trigger mode command After disabling External Trigger mode and providing one more trigger an edit box will show up with the current delay setting see figure below The delay setting can be changed in this edit box 42 to 2730583 nanoseconds After changing the delay you can enable the external hardware trigger by clicking the Trigger checkbox WavaSoft 6 1 Full 2003 Avantes S N 031199952 a x
108. gs Wa Oxygen Settings E E B x Values Use Low Osgen Standard 20 0 High Oxygen Standard 1700 0 Oxygen Unit Percent K Value om 300 lo Value 500 0 New Calibration Low Oxygen High Oxygen Calculate Save Channel LED Contro f LED Continuously ON an Measure every ay seconds LED ON during Measurement Tet 1 scans to start measuring atter LED ts OH Close Form This dialog is shown after selecting Application Oxygen Settings from the main menu The settings are read from the oxygen ini file on disk You can use different standards and units for each of the 8 spectrometer channels The LED Control option allows you to switch the LED to ON by the software only when a measurement is done This option requires a special version of the LED light source AvaLight LED 475 p14 in which it can be controlled by pin 14 of the DB15 connector in TTL position 100 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Note that it is perfectly possible to enter your own values for K and Ip in the edit boxes and work with these values You can save these values or calculate new ones by performing a new calibration Always save changes with the Save Channel button Remember that the K and Ip values are dependent on many parameters such as probe quality fiber diamet
109. her immediately or in the future Microsoft strongly Windows XP recommends that you stop this installation now and contact the hardware vendor for software that has z Select Co n ti nue A nyway passed Windows Logo testing Continue Anyway Depending on the Windows version the dialog at the right may come up which allows you to browse to the directory where the USB driver is aa located Click the Browse button and browse to m the directory C WINNT SYSTEM32 DRIVERS and double click the AvsUsb sys driver Files Needed E x Type the path where the file is located and then click OF If the computer does not support USB Windows i n 95 Windows NT 4 0 use a standard RS 232 E T Browse cable with male and female DB 9 connectors to connect the AvaSpec to the serial port of the computer Launching the software AvaSoft can be started from Windows Start Menu Under Start programs the group AVANTES Software has been added This group contains two icons With the red V icon AvaSoft is started The AvaSoft Help icon can be used to activate the AvaSoft helpfiles these helpfiles can also be activated from the Help menu after starting AvaSoft 6 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com avantes ES AVANTES Software i Avasoft 6 2 for Avaspec Programs E AyaSoFt 6 2 Help Location C 4va5pecb2 Documents
110. hows all files in the current measure mode In the example at the right the measure mode is scope so the extension of the earlier saved spectra is roh To select graphic files that were saved in another measure mode e g absorbance click E behind the Graph Mode and pick the desired measure mode To select graphic files from another folder or drive click El behind the current foldername If a graphic file is marked by a single mouse click on the filename the comment line for this file appears at the top of the graphical region in the main window Selecting multiple filenames can be realized by using the CTRL or SHIFT key in combination with the mouse If the CTRL key is pressed all the files that are clicked by the mouse will be selected for conversion If the SHIFT key is pressed all the files in between two clicked files will be selected for conversion Select the name of the file s to be converted to J Camp and click the Open button To leave this dialog without converting files click the CANCEL button The text files in J Camp format require an extension DX If for example a file AVANTES0001 trm and another file AVANTES0001 abs are both converted to J Camp the result will be AVANTES0001 dx in both cases 3 1 14 File Menu Exit Closes AvaSoft 22 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com 3 2 Setup Menu Vg AvaSofte 6 2 Full
111. ible to save these parameter settings to and load from a file with the extension coo With this feature it is possible to save for each experiment a different coo file so it can be loaded a next time the same settings are required The left figure below shows the dialog in which the name of the file can be entered after clicking the Save Settings button The right figure below which is displayed after clicking the Load Settings button illustrates how to select an earlier saved coo file Enter Color OF Object settings Filename tx Load Color Of Object settings Filename Opslaan als C data E a el aac o Me E fahm E tell Se J frm fa tall 32 Ham 56 n labE yellow con Ham256 Pe labE yellow coo leja Z leja Fj New coo Z smoothing Z smoothing _ speed E speed ea tim 6 Beshandsnaam Bestandsnaam JlabE yellow cog Opslaan ale type Color OF Object Settings Files Annuleren Bestandstypen Color OF Object settings Files ee After the definition of one or more functions the OK button is clicked to start measuring the CANCEL button to leave the dialog and return to AvaSoft s main window After starting the color measurements by clicking OK the output of the selected color parameters will be displayed against time If the option Display no graphics to speed up data processing has been marked in the function entry dialog the function output will be shown by numbers only which are upda
112. ide sphere or cc uWatt cm Power received per square centimeter outside sphere or ce Energy cm uJoule cm Energy received per square centimeter calculated by multiplication of irradiance with the integration time outside sphere or cc Photon Flux m uMol s m Photons received per second and per square meter see for calculation previous page Photon Flux uMol s Photons received per second at diffuser surface outside sphere or cc Photons m uMol m Photons received per square meter during one integration time cycle outside sphere or cc Photons uMol Photons received at diffuser surface during one integration time cycle Photometry Photometry is the measurement of visible light Unlike radiometry it is not a purely physical measurement and is calculated considering a standard human visual perception This is attained by multiplying the radiometric data by the luminous efficacy curve see figure below and integrating the product over the visible range 380 780 nm Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 73 Avantes website http www avantes com email Info avantes com antes Luminous efficacy curve 700 600 oa Q oO gt Q 300 Luminous efficacy lumens watt 200 100 380 430 480 530 580 630 680 730 780 Wavelength nm The three categories that were defined for the radiometric parameters can also be used for the photometric parameters The
113. ill lose resolution when setting the smoothing parameter to 7 Theoretically the optimal smoothing parameter is therefore 6 Ifa 50 micron slit is installed in the spectrometer the optical pixel resolution will be 3 6 CCD pixels and the smoothing parameter should be set to 1 In the table at the left the recommended smoothing values for the AvaSpec 2048 spectrometer are listed as function of the light beam that enters the spectrometer This light beam is the fiber core diameter or if a smaller slit has been installed in the spectrometer the slit width Note that this table shows the optimal smoothing without losing resolution If resolution is not an important issue a higher smoothing parameter can be set to decrease noise against the price of less resolution micron parameter The best way to determine the optimal smoothing parameter is to look at the resolution with a line source like the AvaLight CAL calibration lamp Displaying the resolution in nanometers at Full Width Half Max can be activated by the menu option Setup Options Full Width Half Max First look at the FWHM value of one or more peaks without smoothing smoothing 0 Then increase the smoothing parameter and look at the effect of the increase on the FWHM value s In order to get an optimal smoothing without losing resolution the smoothing parameter should be increased as long as the resolution does not become worse 3 2 4 Setup Menu Correct for Dynamic Dark AvaSpec 20
114. in window 2 Connect the same fiber and diffuser or integrating sphere that was used during the intensity calibration that will be loaded to the Spectrometer input port 3 Start the Absolute Irradiance Application software by clicking the menu option Application Absolute Irradiance Click the Load Intensity Calibration button A dialog shows up in which the intensity calibration file can be selected Select the file and click the open button 4 Switch to the Irradiance Chart TAB to enter the hardware setup and select the colorimetric radiometric photometric and or peak parameters of interest see section 4 4 5 1 Then click OK 5 Measure the output parameters in the experiment If needed change the integration time such that the maximum in Scope Mode is around 14000 A D Counts Block the light path to the spectrometer and save a dark spectrum If the ir radiance of the light to be measured needs to be displayed against time click the time measurement TAB in the settings dialog as described in section 4 4 6 4 4 3 Load Intensity Calibration By selecting the menu option Application Select Intensity Calibration File Absolute Irradiance Measurement a window Poekenin Ba ff i i l f Zoekenin data is shown in which the intensity calibration that was saved before can be loaded by time ee E 4 Z Ham256 J t132 clicking the Load button An intensity s calibration file contains the da
115. inally a multiplication factor can be entered for scaling purpose After clicking the OK button the integral values are given as shown in the figure below w Avatoll D Sl Full S00 Ava E p j la Gin Galup Ww Appheation nip set Mas SATII DAHA Mwb os E O E heynon ime jag 105 ata a fia Wavelength nent 545 a aster INILVRALIARH RO Masher Pis BNTDGRALOODG ood lampbode ae Smoothing 0 Spline OPF To disable the integral calculation the menu option which is marked as long as the Integrals are enabled needs to be reselected To measure the integral versus time up to 8 different functions can be entered in the History application Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 31 Avantes website http www avantes com email Info avantes com antes 3 2 8 4 Setup Menu Options Autosave Spectra Periodically With this option complete spectra will be saved a ears x automatically in time The following parameters can be set Save As Fast As Possible no screen updates e Time delay before first scan needs to be entered Time delay before first scan in seconds After clicking the OK button AvaSoft waits for this number of seconds before the first scan is saved seconds fio Time delay between scans 60 seconds 5 Number of scans to save e Time delay between scans needs to be entered in seconds This defines the time between saving two subsequent spectra If this number is
116. ing calibration curve also through outliers Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 105 Avantes website http www avantes com email Info avantes com aYantes Adding deleting calibration points Adding and deleting calibration points from to the actual calibration line is very simple and very well visualized in the graphical display To add new calibration points to a calibration just load the calibration file move the cursor to the next row in the table row 5 in our example fill in the sample concentration in the center column and read the absorbance into the first column by clicking the Read Absorbance for point 5 button A new red point will mark the calibration point in the graph New points can be added up to 30 standard samples The Calculate New Calibration Line will display a new red line next to the actual blue calibration line before raising the dialog with the question if the new calibration data should be saved or not This may be helpful to see if the new points are correctly measured or that one or more new points induce a strong increase of the standard deviation For EIS a example in the figure at the right the error was caused by o 0 2 TORE os 0 8 typing in the value of 60 in the center column instead of the correct value of 40 To delete one or more calibration points from the actual calibration just delete the value for that point in the left or second column es Actual Poi
117. ing the predicted values Actual Calibration Filename Master 30 000 29 342 Coet 2 o0000Es000 with the actual concentration values MyFirst cal Load 70 000 69 146 StDev Tareas 95 000 95 813 you can get an impression about the ReadAbcorbanceferpcin2 _ quality of your calibration line The actual calibration line will be automatically loaded the next time AvaSoft is started By clicking the Load button other calibrations that were saved before can be loaded after which the loaded calibration line becomes the actual line x Exit 4 8 2 2 Modifying a calibration The actual calibration line can be modified by changing the fit order of the calibration line or by adding or deleting calibration points Fit order The default fit order is linear Using a second order parabolic curve makes it possible to compensate for non linearity in e g your dip probe To illustrate this the calibration from the previous section was loaded MyFirst cal and the fit order was changed from first order to second order After clicking the Calculate New Calibration Line button the new standard deviation showed a much better fit then the previous linear fit After saving this calibration line to MySecond cal the predicted concentrations showed also a much better fit for the quadratic model However certainly with this few calibration points the second order option should be used with care as it will happily draw a well fitt
118. ints 50 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes Function Independent Parameters Display No Graphics To Speed Up Data Processing Save Function Output C Do not save Function Output Save every fo Seconds Change Output File Save Function Output To File D test History dat M Create Backup File X Cancel Save HCF Load HCF Function Independent Parameters Display no graphics to speed up data processing Below the TAB sheets for function definition an option can be enabled or disabled to speed up data processing by not displaying the graphics during the measurements If an application requires fast data processing e g more than 10 scans per second this option should be enabled If saved to an output file the data can be displayed graphically after the time series experiment has been ended as described in section 4 1 3 History Display Saved History Graph Function Independent Parameters Save Function Output The results of a time series experiment will be saved to an ASCII file if the radio button Save Function Output has been selected A number of seconds between saving can be entered for data reduction in case measurements are carried out over long periods Entering a value of zero results in saving every scan The name of the file to which the data will be saved can be changed after clicking the
119. is entered in the dialog that is shown after clicking the Save button Do vou want to overwrite the color above by the new color below for this product AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes O x 4 3 2 2 Time Series Vee of Object Settings LABChart Time Series Fi r2 ra r rs e r re Spectrometer Channel Illuminant CIE Standard Observer Master CA C D55 C D75 2 degrees B C D50 D65 C 10 degrees M Function Display Settings CL Cdd CY C None X axis time M Y axis Color parameter iC C Fi G C Fi Ca Cda CZ Auto Fixed Auto Fixed amp o Ch OEE on ie Display No Graphics To Speed Up Data Processing Save Function Output eccccccccccececccecccccccccccscccoccosececoccccccecccecececesecesos C Save Function Output Save Settings Load Settings X Cancel With time series color measurements up to 8 color parameters or color differences can be followed simultaneously in a graph against time The time series settings are defined in the dialog above which is shown after clicking the Time Series TAB page The functions F1 to F8 can be selected by clicking the corresponding TAB sheet at the top of this dialog Furthermore a number of general function independent parameters can be entered below of the function TAB sheets The following
120. is not visible at the monitor Use the zoom function if you want to verify if this is the cause of saturation Zoomed in Saturation can also happen at a wavelength range that is not visible because the graph is not at full scale Under all these circumstances the saturation label will be shown in the statusbar of the spectrometer channel for which one or more pixels are saturated During time series measurements History Channel Functions color versus time irradiance versus time the saturation detection will be done only for the relevant wavelength range The relevant wavelength range in color measurements is from 380 to 780 nm For History Channel Functions the relevant wavelength range is set by the user For example when monitoring an integral between 500 and 505 nm saturation detection and notification will be done only for this range If saturated the saturation label will become visible in the History Channel output If the time series measurements are saved online to Excel the font color in Excel will be red for a history channel as long as the output for that channel is saturated 28 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Additional information about the saturated wavelength ranges can be visualized and or saved by enabling the following options Additionally more detailed information can be retrieved in the following ways
121. it illustrates only a few of the functions AvaSoft can handle A list of allowed operators and functions is given below Operators Functions log x natural log log10 x log base 10 exp x e to the power of x sqrt x square root Additionally the wavelength signal for master and slave channel s can be entered by m wavelength in nm master sl wavelength in nm slavel s2 wavelength in nm slave2 etc Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 47 Avantes website http www avantes com email Info avantes com antes For example the function that needs to be entered to follow the intensity of the HG peak against time at the second slave channel 1s s2 253 65 Scripts A scripting feature has been added to allow you to use more complicated calculations with many more mathematical and logical functions Scripts will also allow you to combine the results of several history channels AvaSoft uses the Microsoft VBScript language that comes with your copy of Windows We have included a help file for VBScript for your reference You can reach it from the main menu by selecting Help VBScript It lists among other things all available operators and functions When you edit a script either the previous script for the channel will be shown or a new starter script will be generated if there is no previous script AvaSoft includes a simple script editor The scripts are plain ASCII files n
122. ith AvaSoft XLS the View Spectrum mode can also be used to export the spectra online to Excel section 4 6 If the History Channel Functions are used to track the intensity or wavelength of a peak see below for the Function Definition Peak a graphical feature can be used to visualize the peak changes in a spectrum The number of peaks to display in the spectrum can be entered This number between 0 and 100 is the number of vertical lines that will be drawn in the spectrum each vertical line representing one of the detected peaks in the past e The height of the vertical line 1s equal to the peak intensity e The wavelength at which the vertical line is drawn in the spectrum corresponds to the wavelength of the detected peak e The color of a vertical line represents the oldness of the peak Most recent peak is red going back in time followed by orange green and blue All older peaks are gray colored from light gray oldest peak to dark gray one peak older than blue The time interval at which the vertical lines are updated is the same as the time interval at which the function output is saved to be entered under Save every x Seconds under Save Function Output Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 49 Avantes website http www avantes com email Info avantes com antes For example suppose the function output will be saved every 3 seconds and the Nr of peaks to display has been set to 10 in function F1
123. ive effect on the system speed Although system speed is highly dependent on hardware and Windows activities in the background it should be possible to save complete spectra with an AvaSpec 2048 in about 30 milliseconds this way If only a limited number of pixels need to be transferred this can be brought down to 14 ms With an AvaSpec 102 with TAOS detector these numbers are even much lower 6 to 7 ms for a complete spectrum If the automatic saving needs to be aborted before all spectra have been saved the Autosave Spectra Periodically menu option can be selected again and the value 0 can be entered for the number of spectra to be saved 32 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 3 2 8 5 Setup Menu Options Correct for Drift Correct For Drift by Fis 2 INLINE UV vis Automatic Save Dark by TTL shutter by Spectrometer Channel Introduction When measuring the reflectance of a white reference tile or the transmittance of a reference solution against time the output should theoretically remain 100 noise In practice the output value will not remain exactly fluctuating round 100 but the signal can slowly drift away The cause for this drift in the measurement system can be a change in temperature in the optical bench which causes micro bending of the components that focus the light at the detector but also a drift in the light source that
124. l opening sheets and copying data into cells On modern hardware we have been able to copy complete 2048 pixel spectra to Excel with as little as 50 msec overhead per scan 4 6 Applications Excel Output 4 6 1 Select Source Data III tom Besides enabling Excel output you HRAN RAES JC Ce will have to select your source data Function Type S Maa This re dane A the ie way it C None Integral C User Defined Miew Spectrum Peak would be done without the Excel Measure Mode ou tpu t op tion wi th the inpu t screens Scope C Absorbance Transmittance Inadiance gt you use to define History Channel p Function Definition R i Spectrometer Channel Wavelength range nm Functions and Time Series in the Master From 190 0 To 300 0 Nr of peaks to display 0 Color and Irradiance applications The only difference is the Save Function Output box which is not used by the Excel Output option This part is replaced by a separate dialog which will be described V Display No Graphics To Speed Up Data Processing under i Settings Save Function Output Do not save Function Output Save Function Output X Cancel Save HCF Load HCF 4 6 2 Enable Excel Output Enable the option by selecting Application Excel Output Enable A checkmark will appear before the menu entry Enable to show the status of the option Wg AvaSofte 6 2 Full
125. le 1040201751 toog example lmp After the calibration file has been selected the data can be viewed edited and or saved under a different filename by clicking the View Edit Lamp Data button A dialog with the intensity versus wavelength values for the selected calibration light source is shown as well as a graph in which the intensity is displayed versus wavelength File name My Network P Files of type Aug 04 Avantes website http www avantes com AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc __ data 031101451 __ data simulation 2000120 DH Imp 20001 20 DH Imp nd Lamp Files o Cancel h 71 email Info avantes com avantes x M Yiew Edit Lamp Calibration Data Calibration Filename C AvaSpec62 example lmp Intensity werni nm 300 00 0 48329 310 00 0 69182 320 00 0 96351 330 00 1 30902 340 00 1 73888 350 00 2 26319 360 00 2 89128 370 00 3 63144 380 00 4 49070 390 00 5 47462 400 00 6 58718 410 00 7 03072 420 00 9 20591 430 00 10 71178 440 00 12 34578 450 00 14 10388 460 00 15 9807 Save As 200 400 600 600 1 000 Wavelength nm If needed the data can be edited and saved under another filename by clicking the Save As button Before the changed data takes effect the new filename needs to be loaded with the Select lamp option described above CC UV VIS Fiber or AvaSphere sample port diameter The
126. length and amplitude in the main screen By clicking the red stop button the data acquisition is stopped and the last acquired spectrum is shown in static mode The data acquisition can be started again by clicking the same button which now shows a green Start 8 To save the spectrum in the mode chosen before choose File Save Experiment from the menu or click the Save Experiment button from the button bar 9 To improve the Signal Noise ratio a number of spectra may be averaged To do this the value in the white average box in the main window next to integration time can be increased The value can only be changed in static mode When AvaSoft is acquiring data the average box becomes gray A 8 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes File Setup View Application Help 2 Main Window 2 1 Menu bar The menu s and submenu s are described in section 3 2 2 Button bar Sac Tl litt P z sat EM S S SATI O H 3 Start Start Stop button The Start Stop button can be used to display data real time or to take a snapshot in lt gt gt Cursor button After clicking the cursor button a vertical line is displayed in the graph If the mouse cursor is placed nearby this line the shape of the mouse cursor changes from an arrow to a drag shape If this shape is displayed the left mouse button can be
127. lications the minimum integration time of 2ms is used Laserpulse 5 ns Plasma emission to be analyzed spectroscopically Intensity Minimum Spectrometer integration time 5 ps 50 100 us Sasa time Programmable delay 42 ns to 2730583 ns in 42 ns steps jitter 0 ns This portion of the plasma emission is not included in the integration time To set the delay in AvaSoft select the E m external hardware trigger option in the External Trigger Setting dialog as shown Fare E ae noe Purine delay in Perform scans continuously save first available spectrum if TTL signal is high pin 8 nanoseconds between the output that Perform scans continuously save first available spectrum as Reference if TTL signal is high pin 8 will be given at pin 2 to fire the laser and External Hardware Trigger perform a single scan at rising edge of TTL pulse pin 4 the start of the integration time cycle If you want the spectra to be saved J Automatically save on Hardware Trigger Delay from DO2 to start scan 1000 nanoseconds min 4Z2 ns automatically enable the Automatically l 9 S OK x Cancel max 2730583 ns 38 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes save on Hardware Trigger option Then click OK When measuring in external hardware mode the data acquisition is controlled by the external tri
128. ll display the locus for the 2 or 10 degrees standard observer The measured x y coordinates will be displayed in the diagram and a line will be drawn from the midpoint x y 1 3 through the measured x y to the edge of the locus which represents the dominant wavelength The scope data graph displays the raw A D counts that are received from the spectrometer This graph can be used to determine if a good integration time has been set If the integration time is too low the radiometric spectrum will be noisy if the integration time is set too high the spectrometer detector can be saturated in which case a saturated label will appear in the Scope Data Graph The radiometric graph displays the spectrum for the selected radiometric parameter Just like with selecting the radiometric parameter in the radiometry box the available parameters depend on the settings in the irradiance chart settings dialog In the figure at the right an LED is measured inside an integrating sphere in which case only the Radiant Flux emitted power in np Watt and the emitted energy during one integration cycle in Joule can be selected See also the radiometric table in section 4 4 1 The selected parameter in 450 soo S80 600 650 700 the radiometric graph is also used to determine the peaks in Expand Size wavelength nim the peak measurement box Power emitted uWatt Energy emitted Joule Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 83
129. lumn Hardware setup inside sphere refers to measurements that are done with the light source inside an integrating sphere while outside sphere or cc refers to measurements that are done with a light source at a certain distance from the sphere or with a cosine corrector AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com Total optical energy emitted from a source calculated by multiplication of the power with the integration time outside sphere or cc Radiant Flux Total optical power emitted from a source Power emitted calculated by multiplication of radiant intensity with the solid angle of the light source outside sphere or cc Energy emitted uJoule Total optical energy emitted from a source calculated by multiplication of the power with the integration time outside sphere or cc Radiant Intensity 1 Watt sr Optical power per unit solid angle calculated by multiplication of irradiance with the square distance between point source and diffuser surface outside sphere or cc Radiant Energy uJoule sr Total optical energy emitted from a source calculated by multiplication of the radiant intensity with the integration time outside sphere or cc uWatt Power received at diffuser surface outside sphere or cc Energy received uJoule Energy received at diffuser surface calculated by multiplication of the power with the integration time outs
130. m needs to be saved or loaded before the History Channel Measurements can be started Function Definition The parameters that need to be entered in the Function Definition Box depend on the Function Type that has been chosen Integral User Defined View Spectrum or Peak Function Definition Integral Function efinitiori Spectrometer Channel Wavelength range nm Multiply wath Master From 500 0 To 600 0 1 000 In case a function is defined to display the integral versus time the following parameters can be set Spectrometer Channel The default is Master but in case more channels are available this can be changed to Slavel Slave2 up to Slave7 The from and to edit boxes may be changed to specify the wavelength range in nanometer over which the integral needs to be calculated Finally a multiplication factor can be entered The value entered here is multiplied with the calculated integral Function Definition User Defined Function Definition Select UD Function Type f Input Function i Script Edit Script Example log O rmn 456 m 765 4s1 321 0 234 s2 345 m BOO s1 600 If you want to display the output of a self defined function against time a function needs to be defined first You can either enter a user defined input function in the dialog box or assign a script to the history channel Input Functions A comprehensive example of an input function is given in the dialog but
131. m the main menu by selecting Help VBScript It lists among other things all available operators and functions p VBScript SEZ ao g Hide Locate Previous Next Back Foward Stop Refresh Home Print Contents Index Search Favorites VBScript User s Guide User s Guide What Is VBScript Language Reference FileSystemObject User s Guide Adding VBScript Code to an HTML Page Scripting Run Time Reference Regular Expressions Guide BScript Fundamentals VBScript Data Types VBScript Variables VBScript Constants VBScript Operators Using Conditional Statements Looping Through Code VBScript Procedures Finally the third Help submenu is the about box which shows some information about the AvaSoft version that is being used the serial number of the spectrometer that 1s connected the Windows version of the computer and the available memory About AraSoftia o x aYantes Aves one Version 6 2 Full Spectrometer Serial umber 020800451 Copyright 2004 Avantes Windows 2000 Build 2195 Service Pack 4 Physical memor available to Windows 130 612 KB 108 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Appendix A Troubleshooting How to rectify an incorrect USB installation Any USB device requires that the USB drivers need to be installed before the device is connected to the computer If
132. metric Below short background information is given about the colorimetric radiometric and photometric parameters We also describe the definition of the peak parameters that can be measured as well 68 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes The color of light can be expressed by the chromaticity coordinates x y and z These chromaticity coordinates are obtained by taking the ratios of the tristimulus values X Y and Z to their sum Colorimetry y Yy Z X Y Z X Y Z X42 The tristimulus values X Y and Z are computed by KS a Tak S I La A a where k constant 1 Ly 0 00934 IX Spectral irradiance at wavelength A XA YA ZA CIE 1931 or 1964 Standard Observer value 2 or 10 degrees angle at wavelength The tristimulus values X Y and Z and the spectral irradiance are computed in a wavelength range from 380 nm to 780 nm using a nm interval The CIE1960 UCS color coordinates u and v are calculated by 4x 6y gt _7 V n 2x 12y 3 2x 12y 3 The equation that is used for calculating the color temperature is emperical and assumes a black body radiator p x 0 332 y 0 1858 Color Temp 5520 33 6823 3 p 3525 n 449 p In LED measurements the Dominant Wavelength and Purity also known as HelmHolz coordinates are often used to describe a color The Dominant Wavele
133. miss identically configured history channels To monitor a single level set one threshold value to the desired level and the other one to a value that is out of range e g Min to 99999999 and Max to 1000 or Min to 2000 and Max to 99999999 That way you will only need a single channel per threshold value ee a ee An example of running the History Channel functions together with the Process Control application is shown in the figure at the right In this example two History Channel functions were defined The threshold values for function F1 were set at 99 5 and 100 5 percent transmittance for function F5 at 0 and 58 These values are represented by the horizontal lines in the graphs If you need 16 outputs for setting minimum and maximum thresholds per history channel separately then an additional digital card is required AvaSoft supports the PCI 6503 for this purpose To convert the History Channel Functions output to an analog output signal a DA card is available AvaSoft supports the PC AO 2DC card for this purpose 2 analog outputs 8 digital Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 91 Avantes website http www avantes com email Info avantes com oVantes AvaSoft XLS is an add on application that enables Avasoft Full to output history channel data and or complete spectra to Microsoft Excel It uses OLE Automation which is a technology that enables AvaSoft to remotely control Exce
134. n the open file dialog the radiometric spectra that were saved before will be listed By single clicking a file name the comment for this file will be displayed in the graph By double clicking the file name or by clicking the Open button the radiometric spectrum will be displayed This can be repeated for a second filename which allows you to compare the output of the two spectra graphically The online measured spectrum will be displayed in the same graph and can be compared to earlier saved spectra as well 84 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Power emitted Natt v IRRCHART20005 IRR greenyellow IRRCHART20006 IRR orange 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 Wavelength nm The filename and comments for the displayed spectra appear on top of the graph the Eee ae text colors are the same as the colors used to draw the spectra By clicking the blue Sameer a met or green filename with the right mouse button a popup menu for this radiometric Convert te Excel spectrum is presented with the menu options shown in the figure at the right Remove From Chart v irradiance Chart Measurement Info E ioj x The Show Info menu option displays the hardware settings measurement parameters and save settings for Hardware settings f the spectrum as shown in the dialog at the left Spect
135. n be displayed and saved in three ways e Inthe main Window the data can be displayed as spectral irradiance in pWatt cm nm versus wavelength e The measurement results can also be displayed in an irradiance chart figure above which shows the chromaticity diagram the irradiance spectrum the spectrum in scope mode and many parameters for colorimetry photometry radiometry and peak measurements Up to 10 different radiometric parameters and or wavelength ranges can be selected for which the output will be displayed e In Time Measurement mode up to 8 functions can be displayed simultaneously against time For each function a different radiometric photometric color coordinate or peak output parameter and or wavelength range may be selected as well as a different spectrometer channel Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 67 Avantes website http www avantes com email Info avantes com aYantes Before irradiance measurements can be done an intensity calibration is required The intensity calibration contains the data transfer function for each pixel The data transfer function is used to convert the scope data A D Counts into irradiance data in uWatt cm To be able to calculate the transfer function a calibrated light source with known output in uWatt cm nm needs to be available 4 4 1 Background When saving the A D Counts with the reference light source on and off we know the relation between
136. n most cases reference is saved manually by clicking the white reference button This reference data will be saved automatically if this external trigger option is selected and the status of pin 8 is HIGH 5V at the moment that a new spectrum is available For the required TTL signal duration and timing see the above description for saving spectra at high TTL signal External Hardware Trigger perform a single scan at rising edge of TTL pulse pin 4 By selecting the external hardware trigger option the data acquisition mode of the spectrometer is changed There will be no data acquisition until a TTL pulse is received at pin 4 of the DB15 connector The delay between the rising edge of the TTL pulse and the start of the integration time cycle depends on the spectrometer type as shown in the table below Spectrometer Type Minimum Delay us Maximum Delay us Avaspec 102 1000 1500 Avaspec 256 1000 1500 w For example the integration time as set in AvaSoft starts for a standard AvaSpec 102 after 1 0 to 1 5 ms after receiving the TTL pulse at pin 4 of the DB15 connector After the integration time expires the data is processed by AvaSoft and the spectrometer waits for a next pulse The external hardware trigger is useful to observe short pulse events 1 e laser pulses etc To measure a laser pulse the AvaSpec 2048FT includes an option to send out a TTL pulse at pin DO2 to fire the laser just before the integration time cycle s
137. n ordered together with the color application add on Color Chart button The Color Chart button allows you to switch directly to the Color Chart screen and starts measuring immediately E LOR ES m Color of Object versus Time button The Color of Object versus time button allows you to switch directly to the time measurement of color parameters and starts measuring immediately 2 3 Edit bar Integration time me f AVerage f Wavelength im 546 80 When AvaSoft is acquiring data the edit fields are gray and non editable By clicking the red STOP button data acquisition is stopped and the edit fields become white and editable The edit bar shows the following parameters Integration time ms This option changes the CCD readout frequency and therefore the exposure or integration time of the CCD detector The longer the integration time the more light is exposed to the detector during a single scan so the higher the signal If the integration time is set too long too much light reaches the detector The result is that over some wavelength range the signal extends the maximum counts 16384 or in extreme case shows as a straight line at any arbitrary height even near zero Entering a shorter integration time can usually solve this Try to adjust the integration time such that the maximum count over the wavelength range is around 14500 counts When at minimum integration the signal is still too high an attenuator a neu
138. nd z were defined in 1931 and are known as the 2 degrees standard observer values The 2 degrees corresponds with the angle of vision that was used in the experiments to determine these standard observer values In 1964 the CIE recommended the use of different observer values for a higher correlation with visual perception for large samples These are known as the 1964 supplementary standard observer or 10 degrees observer values Both standards are still in use and in AvaSoft the CIE standard observer can be selected by clicking one of the radiobuttons The default is 2 degrees Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 63 Avantes website http www avantes com email Info avantes com antes e Function display settings For each function one of the color parameters can be selected Functions for which the color parameter has been set to None will be disabled If one of the color difference parameters dL da db or dE 1s selected the reference color to which the measured color needs to be compared needs to be defined as well Therefore the reference color definition box figure below will be displayed if dL da db or dE is clicked Reference Colo EEEE Current Reference Color y le O test data example pct DB 36 29 aREF E 0 45 LREF a 66 LREF Select Select Current Reference Color from Table Add Add Current Reference Color ta T able ProductiD pello For each function a differen
139. ngth can be calculated for a measured sample point S with chromaticity coordinates Sx Sy by drawing a straight line from the midpoint in the chromaticity Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 69 Avantes website http www avantes com email Info avantes com antes diagram E with x y 0 333 through S towards the edge of the Chromaticity Diagram spectrum locus The points at the spectrum locus correspond with a wavelength and the interception of the straight line through E and S with the locus 1s called the Dominant Wavelength Purity is the distance from the midpoint E to the sample point S divided by that from the midpoint E to the spectrum locus DW Purity E S E DW The method described above is used for all colors with a Dominant Wavelength from 380 to 699 nanometer If the x y coordinates are in the triangle area encompassed by the 3 points E A and B then the Dominant Wavelength can not be calculated because the interception point through E and S with the spectrum locus between A and B does not correspond with a wavelength In that case the Complementary Dominant Wavelength CDW is used The line from E through S is extended backward in order to determine the Complementary Dominant Wavelength CDW CIE 1931 2 degrees standard observer Chromaticity Diagram Power emitted aWatt v Peak Measurements A typical spectral power distribution of a green LED is shown in le the figure
140. nsmittance is mathematically equivalent to the percentage of reflectance and can also be used for reflectance experiments see also section 3 2 8 11 42 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 3 3 4 View Menu Irradiance Mode If the Absolute Irradiance Measurements Module has been ordered with AvaSoft this option will show the absolute energy output in uWatt cm nm An elaborate description about the experimental setup in case of absolute irradiance can be found in section 4 4 If the absolute irradiance application is not available a light source of known color temperature is needed as a reference for example the AvaLight HAL with color temperatures of 2850K at default jumper setting The relative radiance energy at wavelength A is then calculated using the current sample the reference and the dark data sets S B sample dark Where B is the computed component of the spectral distribution of the blackbody radiant emmittance at user selected temperature in degrees Kelvin divided by the current reference data at wavelength How to take relative irradiance measurements with AvaSoft 1 Start the AvaSoft software and click the Start button in the main window 2 Connect a fiber to the Spectrometer input port 3 Adjust the Smoothing Parameters in the Setup menu to optimize smoothing for the Fiber Slit diameter that is used
141. ntroid wavelength Centroid intensity C Lumen fe To mor om Candela C Peak wavelength Peak intensity X axis time setting Y axis parameter setting C Seconds Autoscale Y axis during measurements fo Display Last fi Minutes noaoo C Hours Preset Y axis scale to a fixed level eee Display No Graphics To Speed Up Data Processing Save Function Output Do not save Function Output C Save Function Output X Cancel Save ICF Load ICF Enable Disable function The radiometric parameters can be set after clicking the Enable button Hardware Setup The Hardware setup setting is an important parameter because as described in section 4 4 1 background it determines the radiometric and photometric parameters that can be measured as well as the way these are calculated If an AvaSphere is used it is important to know if the light source is measured inside the sphere like often is done with LED s or outside the sphere This setting can be entered after clicking the AvaSphere button and then inside or outside sphere Spectrometer Channel If the spectrometer system contains one or more slave spectrometer channels for which also an intensity calibration has been loaded or performed the spectrometer channel on which the output parameter will be measured can be selected from the drop down box at the left showing by default Master Parameter The number of available paramet
142. nts Actual Line a New Points New Line 4 8 3 Enabling the application Enable the application by selecting Application Chemometry Enable from the main menu A checkmark will appear before the menu entry to show that the option is enabled A separate Concentration display will appear for each spectrometer channel a alinizi enabled Master mmol l 2 1 10 x After enabling the chemometry v ES Channel Function Entry application you can select mo dro r3 Fa lrs lre jer lre Chem as a function type in the ee Function Entry dialog of the C None User Defined C Peak A C Integral C View Spectrum Cher History application Remit 106 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes After clicking the Help Contents menu option the AvaSoft FULL manual will be displayed in HTML format At the left side the Help Contents displays all sections to which the user can browse to a specific topic E AvaSoft Help E P olx o amp Hide Back Print Options Contents Search Chemometry Application 0 Contents AQ Quick Start A Button Bar AvaSoft Chem was developed to enable on line concentration measurements AQ Ecit Bar with a spectrometer system a oe AY Graphical Region According to Lambert Beer s law there is a linear relationship
143. nual doc 5 Avantes website http www avantes com email Info avantes com aYantes After all files have been installed the Installation Complete dialog shows up It s strongly recommended to restart the computer before starting AvaSoft Connecting the hardware Connect the USB connector to a USB port on your computer with the supplied USB cable If the spectrometer type is an AvaSpec SPU put the switch into SPU position If the AvaSpec spectrometer requires a 12V power supply connect the AvaSpec to the 12 V power supply Windows will display the Found New Hardware USB device dialog and starts searching for the driver this can take a few minutes Under Windows XP a window will be Der cd Nata Liat heaton MAR displayed that the Avantes Spectrometer board Pica amenorrhea has not passed Windows Logo testing to verify compatibility with Windows XP which indicates that the manufacturer of the USB rN The softwere you are installing for this hardwere driver did not send it to Microsoft and also Avantes Spectrometer board paid Microsoft for testing of this driver as it will be the case for all drivers developed for Hardware Installation has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair Windows 2000 which also work fine under or destabilize the correct operation of your system eit
144. oYantes AVASOFT for AvaSpec 102 256 1024 2048 version 6 2 USER S MANUAL Wg AvaSoft 6 2 Full 2004 Avantes S N 031101451 a laj x File Setup View Application Help sop BM S SATI DO M amp S Melb B Integration time ms fio Average ho Wavelength inmi 435 94 Master 40 Master File AVANTESOOO2 ROH Amplitude 13025 Smoothing 1 Spline OFF Aug 04 Avantes website http www avantes com email Info avantes com antes 0 AVASOFT INSTALLATION 1 QUICK START MEASURING AND SAVING A SPECTRUM 2 MAIN WINDOW 2 1 Menu bar 2 2 Button bar 2 3 Edit bar 2 4 Graphical region 2 5 Status bar 2 6 Find peaks or valleys by CTRL or SHIFT left mouse button click 3 MENU OPTIONS 3 1 File Menu 3 1 1 File Menu Start New Experiment Sz File Menu Load Dark 3 1 3 File Menu Load Reference 3 1 4 File Menu Load Experiment 3 1 5 File Menu Save Dark 3 1 6 File Menu Save Reference 3 1 7 File Menu Save Experiment 3 1 8 File Menu Print 3 1 9 File Menu Black and White printer 3 1 10 File Menu Display Saved Graph 3 1 11 File Menu Convert Graph to ASCII 3 1 12 File Menu Convert Graph to ASCII Equi distance 3 1 13 File Menu Convert Graph to J CAMP 3 1 14 File Menu Exit 3 2 Setup Menu 3 2 1 Setup Menu Hardware 5 2 2 Setup Menu Wavelength Calibration Coefficients 3 2 3 Setup Menu Smoothing and Spline 3 2 4 Setup Menu Correct for Dynamic Dark AvaSpec 2048
145. ocedure will be used to move to the closest valley v Select Graph OF E3 If more than one spectrum is being displayed a dialog as shown at the sa o a cee sta s s needs to be moved to tne next peak right pops up in which the spectrum for which the peakfinder needs to be then click OK activated can be selected out of all displayed spectra i ea A eseseseeseeesess C AVANTESOO36 ROH C AVANTESOO35 ROH 14 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes In sections 3 1 to 3 3 the main menu options File Setup and View Menu and their submenu s are described in detail 3 Menu Options 3 1 File Menu Wg AvaSofte 6 2 Full 2004 Avantes S N 020800451 File Setup View Application Help Start Mew Experiment ISs SATIIOI Average ce WAVE Jave Load Print Black and White printer Display Saved Graph Convert Graph Exit 3 1 1 File Menu Start New Experiment After selecting this option a dialog box appears in which a new experiment name can be entered The experiment name will be saved as a filename with the extension kon This extension does not need to be entered After clicking the save button Enter New Experiment Name the current filename will be built up from the experiment name that has been entered and a sequence number starting at 0001 Example if the experiment name is
146. oft IRRAD is not available shows five columns The first column gives the wavelength in nanometers The second to fourth column give respectively the dark reference and scope data The fifth column shows the calculated value for relative irradiance 3 1 12 File Menu Convert Graph to ASCII Equi distance This option requires that graphic files were saved earlier by using the option File Save Experiment After selecting the option File Convert Graph To ASCII Equi distance the wavelength range for which the Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 21 Avantes website http www avantes com email Info avantes com antes data should be converted and the distance between two successive data points can be entered in the dialog as shown Mf Export to ASCII equidistance setting Oo x at the right After clicking the OK button you can select the Se eee 200 00 eee files which need to be converted Selecting these files is the e000 same as for converting to ASCII without the equi distance a el cal a tel feature section dsdal 1 Distance between datapoints fi od nanometer Also the information in the header file is the same as for converting to ASCII without the equi distance feature The Cancel data in the TRT TAT TTT and TIT files is presented in two columns The first column gives the equally spaced wavelength in nanometers The second column shows the interpolated value for scopedata
147. on Select the spectrometer channel that will be calibrated the calibration lamp file and enter the diameter of the fiber cosine corrector or integrating sphere sample port that 1s used as described in section 4 4 4 Turn on the reference light source e g AvaLight HAL CAL or AvaLight HAL CAL ISP If a cosine corrector 1s used at the end of the fiber mount it directly on the reference light source If an integrating sphere is used at the end of the fiber put the integrating sphere sample port over the light output port Verify that the calibration lamp is ON for at least 15 minutes and click the Start Intensity Calibration button Try to adjust the integration time while looking at the reference light such that the maximum count over the wavelength range is around 14000 counts It s also possible to let AvaSoft search for an optimal integration time by clicking the JAC button Adjust the Smoothing Parameter to optimize smoothing for the Fiber Slit diameter that is used If a good reference signal is displayed click the white Save Reference button A white line will mark the reference spectrum Then switch off the calibration lamp wait until the spectrum becomes flat near the bottom of the scale and click the black button to save a dark spectrum A black line will mark the dark spectrum Click the Save Intensity Calibration button A dialog shows up in which the current settings in this intensity calibration
148. on is displayed at the right in which the keyword is marked in the text E AvaSoft Help E io a amp P Hide Back Print Options Contents Search Type in the keyword to find concentration List Topics Calibration Settings in AvaSoft Chem Actual Ports Actual Line New Ports New Line Select Topic to display Calibration Settings in AvaSoft Chem Chemometry Application Enabling AvaS oft Chem Quick Start How to make concentration me Quick Start How to take oxygen measurem Start New Caltvation Clear Data Fit Order of Calibration Line Fet Dode Uraa Delad Second Order Quadatic 0 000 100 000 100 451 Coal 2 ocooe coo 200 000 198 800 St Dev Taras 30 000 30064 ete 0 106 The dialog above is shown after selecting Application Chemometry Settings for the first time An example calibration file example0 cal has been 3j arantad in tha faldar Saharna trrhich halda the data that in nranantad in tha Display Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 107 Avantes website http www avantes com email Info avantes com antes As described in section 4 1 1 the History Channel Functions have been extended with the possibility to define a function with the support of a VB Script We have included a help file for VBScript for your reference You can reach it fro
149. only E a Setup Menu Subtract Saved Dark 3 2 6 Setup Menu Strobe Enable DO1 EAT Setup Menu 1 kHz Enable DO2 3 2 8 Setup Menu Options 3 2 8 1 Setup Menu Options Check on Saturation 3 2 8 2 Setup Menu Options Full Width Half Max 3 2 8 3 Setup Menu Options Integrals 3 2 8 4 Setup Menu Options Autosave Spectra Periodically 3 2 8 5 Setup Menu Options Correct for Drift 3 2 8 6 Setup Menu Options Automatic Save Dark by TTL shutter 2 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Avantes website http www avantes com Aug 04 email Info avantes com 12 13 13 14 3 2 8 7 3 2 8 8 3 2 8 10 3 2 8 11 antes Setup Menu Options External Trigger Setting Setup Menu Options Auto configure Integration time 3 2 8 9 Setup Menu Options Use Custom Reflection Reference Setup Menu Options Suppress Save Comments Setup Menu Options View Reflectance instead of Transmittance 3 3 View Menu 33l View Menu Scope Mode 3 3 2 View Menu Absorbance Mode 3 3 3 View Menu Transmittance Reflectance Mode 3 3 4 View Menu Irradiance Mode 3 3 5 View Menu Channel 3 3 6 View Menu Change Graph Scale RN View Menu Graphic Reset 3 3 8 View Menu Autoscale Y axis 3 3 9 View Menu Goto Preset Scale 3 3 10 View Menu Grid Enable 3 3 11 View Menu Progress Bar Enable 4 APPLICATIONS 4 1 Applications History Channel Functions 4 1 1 History Application Function Entry 4 1
150. oxygen Although zero oxygen level is recommended for the low concentration any value can be used for the standards as long as they are sufficiently different The levels for the low and high oxygen standard as well as the unit need to be entered in the Oxygen Settings dialog The settings dialog is opened by selecting Application Oxygen Settings from the main menu The probe must be shielded from ambient light to obtain reliable data To determine if ambient light is entering the probe the blue LED can be turned off The spectrum should appear to be flat near the bottom of the scale First save a dark level switch off the LED light source and click File Save Dark or click the Save Dark button in the button bar Then start with the standard in the lower end of your oxygen range Select a suitable setting for integration time try to get a large dynamic range Higher oxygen levels will give lower signal levels Averaging can be increased to e g 10 to improve the stability of the signal 5 Your displayed spectrum should now like the figure below The peak to the left is the 475 nm LED peak It can be higher or lower than your fluorescence peak depending on the oxygen probes The peak in the middle is your fluorescence peak at about 600 nm PEE BESS win SS ae He SATI EHO kib E H f e aau eS ee 6 If you have a stable signal click the Low Oxygen button and ackno
151. ph To select this region click the left mouse button in the white graphics region and drag it downwards and to the right After releasing the left mouse button both the X and Y axis will be rescaled to the new values of the selected region 52 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Zoom out drag with the left mouse button within the white graphics region but in stead of dragging the mouse downwards and to the right drag it into another direction After releasing the mouse button both the X and Y axis will be reset to their default values Move X Y dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right 4 1 3 History Application Display Saved History Graph If time measurement data have been saved the resulting ASCII file can be displayed graphically by Zoekenin test selecting this file in the dialog that is shown after clicking the Display Saved History Graph menu option After clicking the Open button the function output of the functions with function type Integral or User i h Bestandsnaam Histor dat Openen ener Defined is displayed against time Both X and Y axis oen a Bec sadenen History Channel Datafile are set to their full scale which is determined by the P Bataia Annuleren minimum and maximum values in th
152. presents the number of the slave channel for which the reference data has been saved 0 represents the master channel The reference data files will be saved in the experiment directory that has been picked by the option File Load Experiment or File Start New Experiment 16 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 3 1 7 File Menu Save Experiment With this option spectral data is saved All graphic files will be saved in the experiment directory that has been picked by the option File Load Experiment or File Start New Experiment Saving graphic files if one spectrometer channel is enabled First a window appears in which a line of comments can be entered to the saved graph Next two files will be saved the first file contains the saved spectrum data The name of this first file starts with the experiment name directly followed by the sequence number of the saved spectrum The extension of this first file depends on the current measuring mode as shown below Extension Mode ROH Scope Mode ABS Absorbance TRM Transmittance Reflectance IRR Irradiance The second file contains the line of comments which may have been added to this graph The name of this second file is except for the extension the same as the name of the first file experiment name and sequence number The extension of this second file also depends on the measuring mode as
153. r channel are saturated Slavet File AVANTESOOOS ROH Amplitude 261 50 Smoathing g Spline OFF Saturated Master File 4VANTESOOOZ ROH Amplitude 3613 7 Smoothing oO Spline OFF Saturated This is useful for measurements in transmittance absorbance and irradiance mode because in these modes saturation can not be observed by looking at the number of counts like in scope mode But even in scope mode a spectrum can contain saturated pixels also when this is not directly obvious from the graph Examples are Averaging If the measured spectrum is the average of a number of scans some scans may contain saturated pixels while the end result does not show a graph that reaches the maximum counts Smoothing The maximum pixel value of a peak can be saturated but is averaged with neighbor pixels which may not be saturated The correct for dynamic dark algorithm subtracts the dark values that are measured at the optical black pixels from the spectral data Therefore the saturation level of 16383 counts will never be reached with correct for dynamic dark ON The saturation detection in AvaSoft is done before the data is corrected for dynamic dark so it will also detect saturation with dynamic dark ON Monitor resolution The CCD contains 2048 pixels which is a lot more than the monitor pixels in the graph Since not each CCD pixels can be drawn at the monitor a sharp peak at one CCD pixel can be saturated although this
154. re at the right the sample channel will be measured during most of the 60 seconds Once every 60 seconds the FOS switches to the FOS reference channel to take a new FOS reference spectrum that will be used for the correction After clicking OK in the FOS Settings dialog the FOS will display one of the two dialogs below As long as the sample channel is active left dialog the measurement results will be processed as usual As long as the reference channel is active right dialog the measurement results will be used to update the reference spectrum and the A D Counts of this spectrum will be displayed in the dialog FOS 2 Ref Channel FOS 2 Sample Channe Time left until next FOS channel switch 0 00 25 Time left until next FOS channel switch 0 00 27 500 550 BOO B50 OO T50 Wavelength nm Corect for Drift Reference Channel Slave1 This option is available if the spectrometer system has one or more slave Corect Channels channels One spectrometer channel will be used as a reference channel which will continuously measure the reference spectrum e g the white tile in reflectance measurements or the cuvette holding the reference solution in Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Avantes website http www avantes com X Canos Correct for Drift by Spectrometer Channel Slave antes transmittance measurements Changes in this reference signal e g
155. readings in the Chemometry Settings form section 4 8 2 You can also just use a previous calibration line as it can be saved to disk under a user defined filename and reloaded at the start of the application 8 Enable the Concentration measurements by selecting Application Chemometry Enable from the main menu After the application is enabled it is also possible to select Chem as a History Channel function NO GN 102 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes 4 8 2 Calibration Settings The dialog at the right is shown after selecting Application Chemometry Settings for the first time An example calibration file example0 cal has been created in the folder chem which holds the data that is presented in the dialog Note that the example is only illustrative You need to measure a number of samples with known concentration to create your own calibration file s as described in section 4 8 2 1 After a calibration has been saved it will be loaded automatically the next time the M Chemometry Settings Select Variables Channel Peak at 505 0 nm Width 10 0 nm Conc Unit mmol Start New Calibration Clear Data 03 Absorbance 0 1 0 2 0 4 Actual Points Actual Line a New Points New Line 05 0 6 Fit Order of
156. red If the function output is a color difference value like dE in the figure above then a second color indicator panel is shown for the reference color If time measurement data have been saved the resulting ASCII file can be displayed graphically by selecting this file in the dialog that is shown after clicking the menu option Application History Display Saved History Graph After clicking the Open button in this dialog the function output is displayed against time Both X and Y axis are set to their full scale which is determined by the minimum and maximum values in the list Zoom features In each graph the same zoom features apply as in the main window except for scaling the Y axis with the mouse wheel However zooming in over the X axis while the measurement is running and the X axis 1s already scrolling will not be possible because in that case the X axis is updated with each new scan Clicking the pause button to take a snapshot will solve this problem Zoom in select a region to be expanded to the full graph To select this region click the left mouse button in the white graphics region and drag it downwards and to the right After releasing the left mouse button both the X and Y axis will be rescaled to the new values of the selected region Zoom out drag with the left mouse button within the white graphics region but in stead of dragging the mouse downwards and to the right drag it into another direction After
157. rflow internal memory with scans in Export mode Therefore a limit to the number of scans to store in Excel has been built in Before a new sheet is added the amount of memory Excel uses is determined If this number is larger than half the physical memory installed in the machine output to Excel will stop On a 128 MB machine this corresponds with 7 8 complete sheets of 256 columns and 2000 rows roughly 2000 full scans Limiting the wavelength range will extend your range of scans accordingly Of course adding more Ram to your machine will also help raise this limit We have however seen limitations in the amount of memory that Excel can use It looks like there is an internal limit that is hit before internal memory runs out In a machine with 512 MB the limit corresponds with about 16 sheets of complete 2000 pixel spectra After this Excel issues an Out of Memory message and is no longer functional E g saving your data to disk is not possible anymore This is only an issue on machines with large amounts of memory 256 MB 512 MB For greatest speed it is important to give the focus to Excel If you set the focus to AvaSoft Windows will dramatically lower Excel s priority You can verify this by looking at the Filling Cells progress bar in the lower left corner of your worksheet 98 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Av
158. rmine a peak parameter Photometric parameters do not need a wavelength range because for these parameters the range is always from 380 to 780 nm However since emitted Lumens and Candela are the photometric equivalent of radiant flux and radiant intensity the geometry for emitted Lumens and distance for emitted Lumens and Candela needs to be specified if the source is not measured inside an integrating sphere If a source is measured inside an integrating sphere the radiometric parameters that can be measured are limited to radiant flux uWatts emitted and emitted energy Joule e and the photometric parameter that can be measured in this hardware setup is emitted Lumen Function display settings The parameters that have been set in the functions can be displayed graphically against time The amount of time that will be displayed at the X axis can be set manually under the X axis setting The Y Axis can be set to a fixed range or to Autoscale The Autoscale option will set the Y axis range to the minimum and maximum function values that are in the list of measured datapoints Function Independent Parameters Display No Graphics To Speed Up Data Processing Save Function Output C Do not save Function Output Save every fo Seconds Change Output File Save Function Output To File D S W5FULL peaktrack data InrT ime dat N Create Backup File i XX Cancel Save ICF Load ICF Function Independ
159. rom both the Color and the Irradiance applications 4 5 1 Digital Output signals AvaSoft supports 8 built in digital outputs on the AvaSpec one per history channel The output pins on the High Density 15 pole Sub D connector which are used for the process control application are listed in the table below So pin 5 is used to monitor the thresholds that are set for History Channel Function F1 etc HD DB15 pins used by ae Process Control connector es co u On us N Q J oO An IC DB15 2 interface cable is available to connect all pins to your process equipment 4 5 2 Using the Process Control Application in AvaSoft The Process Control application needs to be activated by selecting Application Process Control Enable A checkmark will appear before the menu entry to show that the option is enabled Wa AvaSoft 6 2 Full 2004 Avantes S N 020800451 File Setup View Application Help stop ty oo ry ede Wavelength Calibration Integration time ms Color Measurement Wavelength nim e Process Control Enable Absolute Irradiance Measurement Digital Output Settings Excel Output j Oxygen d Chemometry 90 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes To set minimum and maximum threshold values for each of the History functions select Application
160. rometer 021200652 Channel Master DetectorT ype amp vaSphere source inside sphere Diameter sampleport Ava pere 10000 micron The Measurement parameters displays the unit of the intensity of the displayed saved spectrum This can be a different unit than the selected unit for the online measured spectra Also if comparing two saved spectra graphically be aware that these were saved in the same radiometric unit Heasurement parameters Intensity Measured in Power e piw att Intime 61 ms Avg 10 Smoothing 6 Save settings Filename C VWavaSpecbe data 021200654 ARACHAR T20006 1AA The other menu options in the popup menu Convert to ASCI and Convert to Excel can be used to export the data from the binary file with extension IRR to a textfile txt or to a file that can be opened with Excel xls The Remove from Chart menu option clears the saved spectrum At the right side of the button bar the integration time and number of scans to average can be changed The Auto Configure Integration time button can be used to let AvaSoft search for integration time such that the maximum output in scope mode A D Counts will be around 14500 counts Do not forget to save a new dark spectrum black button after changing the integration time 4 4 6 Time Series Measurement Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 85 Avantes website http www avantes com email Info avantes com
161. s Select one or more graphic files to open E l 3 x roh To select graphic files that were Ea saved in another measure mode c O B Objectname AANTESO004 ROH AVANTESOOO2 ROH e g absorbance click behind My Network P Objects of type Graph Scope Mode Cancel the Graph Mode and pick the me desired measure mode To select graphic files from another folder or drive click E behind the current foldername If a graphic file is marked by a single mouse click on the filename the comment line for this file appears at the top of the graphical region in the main window Selecting multiple filenames can be realized by using the CTRL or SHIFT key in combination with the mouse If the CTRL key is pressed all the files that are clicked by the mouse will be selected for displaying If the SHIFT key is pressed all the files in between two clicked files will be selected for displaying Select the name of the file s to be displayed and click the Open button To leave this dialog without displaying graphic files click the CANCEL button Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 19 Avantes website http www avantes com email Info avantes com avantes fag Av atoll 6 0 Full 7003 Avantes i E laj In the figure at the right two De Sip Yew ton th graphic files were selected in j m an oa I sitali bE scope mode The comments that were saved with these j2 amp
162. setting The graphical display can be enabled or disabled by a mouse click for each graph separately e Display following Color Parameters The values of the color parameters that are enabled will be displayed below of the charts during the measurements e Save Settings The color parameters can be saved to an ASCII file during the measurements al ci The current name and location of this ASCII file Coy ea Ee labhexyz txt new tet path can be changed by clicking the Change test tet Output File button The next dialog will be displayed is shown under save setting This filename and To start a new file for saving the color parameters enter a filename that does not Bestandsnaam already exists AvaSoft will add the txt Bestandstypen Color of Object Output File zj Anneren extension and click Open If an existing file is selected the color parameters that will be saved are appended to this file This way experiments that were saved before can be proceeded using the same output file e Reference Color Reference Colo GS DB File D Stest dataexample pct Current Reference Color Select Select Current Reference Color from Table LREF 22 24 aREF 57 bREF A 4 Add Add Current Reference Color to Table e en RR ee ee TREN RP HEARED ProductO 568 nm The reference color can be set to compare the L a and b color parameters during the online measurements with
163. stop the time measurements temporarily After clicking the Pause button data acquisition stops and the text on the button changes to a green Start If the Start button is clicked data acquisition 1s activated again and the text changes back to the yellow Pause again The Save Reference and Save Dark buttons have the same functionality as in the main window The Info button shows the dialog in which all parameters can be viewed not edited while the measurement is running gee Foe E o By clicking the Print button the graphics that are displayed can be printed during the run First the printer specific dialog shows up in which the print options can be set For example if only one graph of the four in the figure above need to be printed this graph can be selected by the page s radiobutton All graphics are printed full size on a different page If time measurement data have been saved the resulting ASCII file can be displayed graphically by selecting this file in the dialog that is shown after clicking the menu option Application History Display Saved History Graph After clicking the Open button in this dialog the function output is displayed against time Both X and Y axis are set to their full scale which is determined by the minimum and maximum values in the list Zoom features In each graph the same zoom features apply as in the main window except for scaling the Y axis with the mouse wheel However zoomin
164. t a conveying belt If the AvaSpec 2048FT scanning is controlled by an external hardware trigger signal at pin 4 of the DB15 connector it will carry out the following events after receiving this trigger signal 1 First it will respond by putting a TTL output signal at pin 2 of the DB15 connector This TTL output can be used to fire the laser in the LIBS application 2 The integration time cycle starts a user defined delay after the TTL output to the laser has been given The minimum period for this delay is 42 nanoseconds in which case the laser pulse itself will be measured because the integration time will start 42 ns before the TTL out at DO2 The setting of the delay period can be increased with 42 nanoseconds steps The maximum delay is 2730583 ns A frequently used delay setting in LIBS applications is 1000 ns There is no jitter on the delay period which makes to AvaSpec 2048FT not only suitable for simple classification but also for precise quantitative analytical applications The software programmable delay between the output at DO2 to fire the laser and the start of the integration time is used to avoid the measurement of the first period directly after the laser pulse in which the plasma emission shows a high intensity but without the useful information The figure below shows an example of the plasma emission intensity after the laser pulse The integration time for the AvaSpec 2048FT can be set from 2 ms to 60000 ms In LIBS app
165. t reference color can be selected A detailed description about the DB Select and Add button can be found in the previous section The color parameters that have been set in the functions can be displayed graphically against time The amount of time that will be displayed at the X axis can be set manually by clicking the Fixed radiobutton see figure below If set to Auto the time axis will be set to 1 minute The Y Axis can also be set to Fixed or to Auto The Auto option will set the Y axis range to the minimum and maximum function values that are in the list of measured datapoints Function Display Setting mans time Auto fans Function Value Auto tf Fixed C Seconds e500 Display Last i Ch iiss YMin C Hours YMan 105 0 Function Independent Parameters Display No Graphics To Speed Up Data Processing Save Function Output C Do not save Function Output Save every fo Seconds Save Function Output To File D test CooT ime dat V Create Backup File A OK x Cancel Save Seltings Load Seltings Function Independent Parameters Display no graphics to speed up data processing Below the TAB sheets for function definition an option can be enabled or disabled to speed up data processing by not displaying the graphics during the measurements If an application requires fast data processing this option should be enabled If saved to an output file the data can be displayed graphically after the time
166. t the right 48 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes If you refer to channels that are undefined Function Type None they will be handled as uninitialized variables by VBScript with value 0 This is the case for all uninitialized variables you might use in your scripts Finally a small example to monitor the integral of a peak between 522 nm and 550 nm in which an offset is subtracted The offset area is the area below of the straight line between the output at 522 nm and the output at 550 nm F1 is defined as a user defined input function m 522 F2 is defined as a user defined input function m 550 F3 is defined as the zero based integral between 522 nm and 550 nm The script for function F4 can then be written as Function F4 value If Fl gt F2 Then offset 28 F2 0 5 F1 F2 Else offset 28 F1 0 5 F2 F1 End If F4 F3 offset End Function Function Definition View Spectrum Function Definition Spectrometer Channel Wavelength range Am Master From 400 0 To 500 0 Nr of peaks to display 10 The View Spectrum function does not display function output against time Instead the wavelength range that has been entered in the Function Definition Box will be displayed at the X axis The spectrum for the selected spectrometer channel will be displayed in the selected measure mode In combination w
167. ta which is BE Nieuwe map 5 D2 201 Slave VIS dh necessary to convert the Scope data to Z smoothing 5 Mastemew di Irradiance data If the spectrometer system l speed 3 SlaveT New df has one or more slave channels it is important to know that the calibration data for each spectrometer channel are saved in a Bestandsnaam D211201 Master LIVWIS dr separate file To measure irradiance data at Bestandstypen Intensity Calibration Files 4 tence more spectrometer channels simultaneously the calibration files for each spectrometer channels needs to be loaded first After loading an intensity calibration file a graph is displayed which shows the data transfer function for the loaded channel The irradiance spectrum is calculated by multiplying the measured scope data from which a saved dark spectrum is subtracted with this data transfer function If the intensity calibration has been loaded the Irradiance Chart and Irradiance Time Series tabs will be enabled Calibration tradiance Chart Iradiance Time Series Clicking one of these tabs allows you to change the settings for the irradiance chart or time series measurements after which the measurements can be started by clicking the OK button The irradiance chart and time series settings will be Calibration ha Been loadediionthe floning chanelle described in sections 4 4 5 and 4 4 6 Master Load Intensity Calibratio 76 AVASOFT 6 2 for AvaSpec 102 256
168. tarts delay 42 ns By setting the delay between the TTL out at DO2 and the start of the integration time to a positive value the AvaSpec 2048FT can be used in LIBS application Laser induced breakdown spectroscopy where the laser pulse itself should not be measured but the plasma emission e g 1 us after firing the laser For details about the delay settings for the AvaSpec 2048FT in external hardware trigger mode see below If the white automatic save on trigger box is marked each spectrum that will be recorded in external hardware trigger mode will be saved automatically Make sure that before the External Hardware Trigger is selected and confirmed by the clicking the OK button a 5V TTL pulse can be set on pin 4 To leave the external trigger mode the External Trigger Setting menu option needs to be selected and the None radiobutton needs to be clicked followed by the OK button However the spectrometer firmware will not return to normal acquisition mode before it receives the TTL pulse at pin 4 AvaSpec 2048FT and external hardware trigger Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 37 Avantes website http www avantes com email Info avantes com antes The AvaSec 2048FT has been developed for the LIBS Laser induced breakdown spectroscopy applications but can of course also be used in applications that need a fast response to an external trigger signal such as when measuring products a
169. ted each time a new scan is saved to the output file There are six buttons at the top of this window see figure below an Exit button a Pause Start button a Save Reference button a Save Dark button an Info button and a Print button If the red Exit button is clicked the time measurements are ended and the main window and menu is activated The yellow Pause button can be used to stop the time measurements temporarily After clicking the Pause button data acquisition stops and the text on the button changes to a green Start If the Start button is clicked data acquisition is activated again and the text changes back to the yellow Pause again The Save Reference and Save Dark buttons have the same functionality as in the main window The Info button shows the dialog in which all parameters can be viewed not edited while the measurement is running Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 65 Avantes website http www avantes com email Info avantes com By clicking the Print button the graphics that are displayed can be printed during the run First the printer specific dialog shows up in which the print options can be set For example if only one graph of the four in the figure above need to be printed this graph can be selected by the page s radiobutton All graphics are printed full size on a different page At the right top of each graph a color indicator panel shows the color of the sample being measu
170. test the first graphic file that will be saved in scope mode will be called test0001 ROH the sequence number will be automatically incremented so the next file that will be saved in H scope mode will be called E test0002 ROH etc For detailed Save asiyne Experiment Files E information on graphic filenames see File Save Experiment Note that the dialog allows you to select different folders or drives to save the experiments to as well as creating a new folder name for the new experiment The default folder in which data is saved is called data lt serialnumber gt in which lt serialnumber gt refers to the serial number of the AvaSpec spectrometer that is being used 0208006A1 in the figure above After closing the dialog box by clicking the save button the new experiment name followed by its sequence number is displayed in the lower left of the status bar By clicking the cancel button the old experiment name will be restored File name Cancel A Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 15 Avantes website http www avantes com email Info avantes com antes With this option dark data can be loaded that have been saved before If AvaSoft is in static mode the dark data that will be loaded are shown on the screen first 3 1 2 File Menu Load Dark 3 1 3 File Menu Load Reference With this option reference data can be loaded that have been saved before If AvaSoft is in st
171. the A D Counts and uWatt cm Caldata refcal darkcal Caldata Intensity of the calibrated light source at pixel n in Watt cm from lampfile refcal A D Counts at pixel n that were saved with the reference light source on darkcal A D Counts at pixel n that were saved with the reference light source off When measuring the A D counts received from a light source different from the calibrated light source but of course with the same fiber optic cable and diffuser this relation can be used to measure the intensity at every pixel n in Watt cm If sample is the measured A D counts at pixel n when looking at the sample light source and dark is the measured A D Counts with the sample light source off then the equation for intensity I in Watt cm becomes T Calda ere refcal darkcal If during the intensity calibration a different integration time was used e g 100ms from the integration time during the sample measurements e g 2 ms a factor needs to be added to the equation to compensate for this In the example the factor is 100 2 50 Calculating the intensity in 1 Watt cm from the measured sample spectrum in A D Counts can therefore be done by the following equation sample dark I Caldata J refcal darkcal factor From the irradiance spectrum in uWatt cm a lot of light output parameters can be calculated colorimetric photometric and radio
172. the LREF aREF and bREF of the reference color The color differences can be shown during the measurements by dL L LREF da a aREF db b bREF and or dE V dL da db The reference color can be entered manually under Current Reference Color in the figure above or it can be selected from a database file The database file is a list of reference colors that were saved before to this file all with a unique ProductID By default the name of the database file is empty pct By clicking the DB button a different database file can be selected or a new file can be created 60 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes To start a new database file for saving reference MEL E Ea colors enter a filename that does not already Zoeken in E data amp c exists AvaSoft will add the pct extension and click Open To open an existing file simply double click on the filename or select it and click the Open button The file example pct can be found in AvaSoft s main directory default Bestandsnaam Jexamole pet Dpenen C AvaSpec60 and contains a few measured oeren reference colors Bestandstypen Reference Color Table T Annuleren To select a reference color from the database See Tol file click the select button below of the DB button The contents of the database file will be Product Color
173. the Reflectance name will shown at the Y Axis e The Caption at the Reflectance Transmittance button will change from T to R and the corresponding menu option under View will change from Transmittance mode to Reflectance mode Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 41 Avantes website http www avantes com email Info avantes com 3 3 View Menu Wg AvaSoft 6 2 Full 2004 Avantes S N 031101451 File Setup View Application Help Start Scope Mode Absorbance Mode Integration tit Transmittance Mode Irradiance Mode Channel Graphic Reset Auboscale Y axis Change Graph Scale Goto Preset Scale Grid Enable Progress Bar Enable 3 3 1 View Menu Scope Mode The display is set to Scope Mode showing a real time raw data signal with on the Y axes the read out of the AD converter and on the X axes the calculated wavelength 3 3 2 View Menu Absorbance Mode In Absorbance Mode the absorbance at pixel n is calculated using the current sample reference and dark data sets in the following equation See sample dark ref dark 3 3 3 View Menu Transmittance Reflectance Mode In Transmittance Mode the transmittance at pixel n is calculated using the current sample reference and dark data sets in the following equation r poo 5p ea ref dark As described in section 3 2 8 9 the reference white percentage of 100 can be customized The percentage of tra
174. time see option View Channel then the graphic filename for each channel that is displayed gets a different sequence number For example the result of saving one experiment in triple view mode is three graphic data files and three comment files for instance avs0002 roh spectrum data in scope mode e g Master Channel avs0003 roh spectrum data in scope mode e g Slavel Channel avs0004 roh spectrum data in scope mode e g Slave2 Channel avs0002 rcm comments for the spectrum saved in avs0O002 roh avs0003 rcem comments for the spectrum saved in avs0003 roh avs0004 rcm comments for the spectrum saved in avs0004 roh For each channel a different comment line can be entered To make it easier to select the graphic files later on with the option File Display Saved Graph all comment lines start with a short name for the channel at which the graph has been saved M for Master S1 for Slavel and so on After saving the sequence numbers in this example triple view mode will automatically have been increased to 0005 0006 and 0007 for resp Master Slavel and Slave2 3 1 8 File Menu Print ee a zE Set E Ba SATI I L rigent jaj fee tieng ret ae After selecting the print menu option the background colors in the Ga graphical region will become white If the menu option Black and White i printer see next section has been marked the line style for the spectra will also change from colored to a black A dialog will be sho
175. time that is spent on transmitting the high number of average spectra to the PC WavaSoft 6 2 Full 2004 Avantes S N 031101451 lej xi File Setup View Application Help sop Was SATI 0A wb Integration time ms ao Average fi oo Wavelength nm 588 10 Master File EXTRIG8_0022 ROH Amplitude 1 4975 Smoothing O Spline OFF Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 45 Avantes website http www avantes com email Info avantes com antes Wg avaSofte 6 2 Full 2004 Avantes S N 031101451 Fie Setup view Application Help Stop E History Wavelength Calibration d Integration time m Color Measurement Process Control 4 Applications PESE Wavelength Absolute Irradiance Measurement Excel Output i Oxygen Chemometry 4 1 Applications History Channel Functions 4 1 1 History Application Function Entry With the History Application the output of self defined Fi r2 rs rs rs re r7 re functions or integrals can be eee followed ina graph against time C None Integral C User Defined C View Spectrum Peak Up to 8 functions can be Measure Mod followed simultaneously C Scope C Absorbance Transmittance nradiance A Function Definition The functions are defined In the Spectrometer Channel Wavelength range nm Multiply with dialog at the right which is Master 7 From feo oo 1 000
176. tion 4 7 3 Settings 4 8 Chemometry 4 8 1 Quick Start How to make concentration measurements with AvaSoft Chem 4 8 2 Calibration Settings 4 8 2 1 Start New Calibration 4 8 2 2 Modifying a calibration 4 8 3 Enabling the application 5 HELP APPENDIX A TROUBLESHOOTING How to rectify an incorrect USB installation 4 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Avantes website http www avantes com 97 97 98 99 100 100 102 102 103 103 105 106 107 109 Aug 04 email Info avantes com 109 antes Before you connect the AvaSpec spectrometer to the USB port of your computer you need to install the AvaSoft software first 0 AvaSoft Installation AVASOFT version 6 2 is a 32 bit application and can be installed under the following operating systems e Windows 95 98 Me e Windows NT 2000 XP If the operating system is Windows 95 or Windows NT4 0 use a standard RS 232 cable with male and female DB 9 connectors to connect the AvaSpec to the serial port of the computer Installation program With each new spectrometer system an AVANTES PRODUCT CD ROM is included One of the options in the main menu which is shown after the CD ROM is inserted in the CD ROM drive is to install AvaSoft software After selecting this option a submenu is displayed in which the spectrometer configuration can be selected The AvaSpec 102 256 1024 2048 group of spectrometers should be selected to install AvaSoft for one o
177. to Excel You do not have to close the worksheet or close Excel 4 6 5 Stop Output If you want to stop the transfer to Excel give focus to AvaSoft and press Exit It may take a few seconds before the buttons appear Exit Pause HH Info Fe User Defined Functor 21 800 Scope Mode Output 10 24 Excel will not be closed by AvaSoft Save your work and close Excel the same way you would when working with Excel manually We have included a sample worksheet called Continuous xls which demonstrates linking to the AvaSoft Data sheet In this case two graphs are drawn from the columns for Channel 1 and 2 The graph and data are continuously updated while data is being transferred to Excel Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 97 Avantes website http www avantes com email Info avantes com aYantes fxd Microsoft Excel continuous xls HARMKE 12 x 6 Bestand Bewerken Beeld Invoegen Opmaak Extra Grafiek venster Help 8 x Err EE ET 2 e z ule Sees 4A Tekengebied 4100 3600 4 3100 l am 2100 Tekengebied i Reeks1 g Reeks i E E pA l E ie ee 20 00 300 00 400 00 500 00 600 00 700 00 800 00 900 00 1000 00 1100 00 1208 00 I4l4 gt bi Graphi AvaSoft Data Test sheet Gereed 4 6 6 Limitations and Optimization Notes As all scans are stored in memory it is very easy to ove
178. tral density filter or fibers with a smaller diameter may be used When not enough light reaches the spectrometer likewise a longer integration time should be entered It s also possible to let AvaSoft search for a good integration time by clicking the JAC button or by using the menu option Setup Options Auto Configure Integration time If measurements are done in a mode in which reference and dark data are required all modes except Scope mode then new reference and dark spectra need to be saved after the integration time has been changed Average With this option the number of scans to average can be set A spectrum will be displayed after every scans This spectrum is the average of the scans 12 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com aYantes The wavelength shows the position of the cursor which becomes visible 1f the cursor button is down The amplitude of the signal which is given in the statusbar at the bottom of the main window is the amplitude at the wavelength shown in this field 2 4 Graphical region The graphical region displays the data in an XY diagram with at the X axis the wavelength in nanometers and at the Y axis the detector counts After loading or saving a reference and dark spectrum other units can be selected at the Y axis Absorbance Units Percentage Transmittance or Relative Irradiance Zoom
179. tton which is in the down position during the search or reselect the menu option Setup Options Auto Configure Integration time When the maximum peak is around 14000 counts a dialog is shown in which the new integration time is given Since the integration time has changed new reference and dark spectra need to be saved before switching to transmittance or absorbance mode is possible For this reason this option is only available in scope mode 3 2 8 9 Setup Menu Options Use Custom Reflection Reference In Transmittance Reflectance Mode the transmittance reflectance at pixel n is calculated using the current sample reference and dark data sets in the following equation T c sample dark m ref dark C 1s the Custom Reflectance Ee n _ Reference factor at pixel n In earlier Omne SATI UBSo kb Ek versions of AvaSoft this factor was rtegrtentme ne Average feat oF set to 100 for each pixel and could not be modified In AvaSoft version 6 the custom reflectance reference j factor is default set to 100 but can be E cee set to different values if needed If i tsa the reflectance spectrum of a white EE tens calibration tile is known the data can Ti be read from a file or entered in a table in AvaSoft The resulting custom reference data can be saved and loaded under a user defined filename Click the Enable Custom Standard radio button and then OK in the Custom Reflectance Settings dialog pe
180. tton on this peak If only one spectrometer channel is enabled and there are no earlier saved graphs being displayed then the FWHM value of the marked peak will be shown directly on top of the peak Furthermore this peak will be yellow marked over the width of the FWHM value it may be necessary to zoom in on the peak to be able to view that the peak is marked v Select Graph i Ioj x Select the Graph for which the Full Width Half Max needs to be calculated If more than one spectrum is being displayed a dialog as shown at the right pops up in which the spectrum for which the FWHM needs to be activated can be selected out of all displayed spectra then kek OK In the figure below four peaks have been selected for FWHM calculation three for the Master channel and one for FWHM0001 roh which is an earlier saved spectrum The FWHM f PWHMOOOT ROH values are given in the same color the spectrum 1s drawn in Mg avasofen 5 1 Full 2002 Avantes E l j Fis up Yem Appain Help sa Bese SATI DAAA Meleag o pE E tegration tine nat fing Average ig Waveiangan inet Fea 55 HM 0 3 78 nm HM 0 45 nm Masher Fiy PWHMONZROH Arpkuie IEJ methine t Spine OFF To disable the FWHM calculation the menu option which is marked as long as the FWHM is enabled needs to be reselected 30 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com
181. uare on the left top of the screen with the mouse 7 Turn on the reference light source of known color temperature 2850K for AvaLight HAL with default jumper setting and set up the fiber end that is not connected to the spectrometer so that a good spectrum is displayed on the screen Note that the integration time or fiber type may not be changed while measuring the reference data If there is too much light adjust the focusing of the light source so less light is coupled into the fiber As long as only light from the reference light source and no ambient light is coupled into the fiber this will not influence the spectral distribution only its height Try to set up the fiber such that the maximum count over the wavelength range is around 14000 counts Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 43 Avantes website http www avantes com email Info avantes com antes 8 Save the Reference data This is done by File Save Reference from the menu or by clicking the white square on the left top of the screen with the mouse 9 Note that the whole operation of saving a reference file does not need to be carried out each time a new experiment is started The reference data are saved in a file called ref dat and can be loaded next time by choosing the option File Load Reference After saving or loading reference and dark the irradiance mode can be chosen by clicking the I button or by the menu option View Irradiance mode
182. ue representing the elapsed milliseconds since midnight This value does not have a one millisecond resolution Remember that Windows is not a real time operating system It can however be used as a reasonably accurate indicator of the time that passes between scans Columns C through J contain the 8 different History Channel values For unselected History Channels a value of 0 will be entered in the sheet If you select View Spectrum in your choice of History Channels the sheet will be formatted vertically As Excel only offers 256 columns per sheet we need to write a spectrum which can hold more than 2000 pixels in a column instead of a row and add more columns as time passes 94 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes Ed Microsoft Excel Blad2 EE few 26 CS I l x Bestand Bewerken Beeld Invoegen Opmaak Extra Data Venster Help l pa KD fh G gt ara 10 BZU 20 El _ o A AseESi4a s ae Al z C D E G 41552942 41554194 41554705 41555025 41555326 41555626 41 0 814117432 3 7605896 3 470443726 4 502830505 2 27 7122498 1 30783844 5 080 0 205491943 1 164115906 4 7 23083496 7 342315674 5 006736755 3 205055237 1 196 4 814117432 4 7605696 3 470443726 6 502630505 7 27 7122498 7 307636044 0 919 4 205491943 2 16411590
183. umens or luminous intensity Candela need to be calculated then the distance in meter and geometry of the lamp in steradians can be entered in the irradiance chart window as well The geometry of the MEsperiment setup point SOUTCE 1S default An steradians and Distance from pointsource to detector surface 2 500 meter is needed only for the radiant and Geometry of the pointsource 112 566 steradians luminous flux calculations In this section the following items from the irradiance chart window will be described e Add Edit radiometric parameters e Graphics e Button bar The other output parameters are displayed according to the settings in the irradiance chart settings dialog Background information about all output parameters can be found in section 4 4 1 Add Edit radiometric parameters There are two ways to monitor the radiometric parameters The first one is by defining up to ten different parameters or wavelength ranges in the radiometry box at the right side of the irradiance chart window The second one is by selecting a radiometric parameter in the graph after which the spectrum for the selected parameter will be displayed see below under graphics To define an output parameter at one of the ten lines in the radiometry box simply double click the line or click the Edit button on top and enter the line number A dialog will be shown in which the radiometric parameter and wavelength range can be specified
184. up is source inside sphere the detailed setting for the radiometric parameters emitted power in uw Watt or emitted energy in uJoule is done while measuring in the irradiance chart dialog The same difference in hardware setup determines which photometric parameters can be measured If the hardware setup is not source inside sphere the luminance in Lux can be measured The Luminous flux 80 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes the photometric equivalent of radiometric Power can be measured at the diffuser surface but it can also be calculated how much lumens are emitted by the source An important assumption is that the source should be an isotropic point source In this setup the Luminous Intensity in Candela can be measured as well If the hardware setup is source inside sphere only the Lumens emitted by the source can be measured Photopic Parameter Received by detector surface e luminance Lux Lumers nf Luminous Flux Lumens Luminous Intensity Candela T Luminous Flux Lumens eotropic pointsource assumed Distance from source to detector can be set while measuring Emitted by a source The peak parameter group allows you to enable disable the following peak information Peak Parameters W Center Wavelength nmn f Centroid hm j Absolute Peak nm fw PavHM W Arplitude at Center wavelength
185. used to drag keep left mouse button down the line with the mouse towards a new position Moving this line shows the corresponding values of wavelength and amplitude in the main screen As an Set Big Step Cursor Movement E alternative for dragging the line the small step and big step arrow Set cursor movement in nm for lt lt and gt gt buttons may be used or the left and right arrow keys on the keyboard 5 00 The step size for the arrow buttons can be changed by holding down the CTRL key while clicking at a single or double arrow button __ Caneel E Save reference and dark buttons The reference button is the white button at the left top of the screen It needs to be clicked to save the reference data The same result can be achieved with the option File Save Reference The dark button is the black button at the left top of the screen It needs to be clicked to save the dark data The same result can be achieved with the option File Save Dark m Save experiment button By clicking the Save Experiment button an experiment is saved The same result can be achieved with the option File Save Experiment Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 9 Avantes website http www avantes com email Info avantes com antes By clicking the Print button a graph that is displayed on the monitor will be printed The same result can be achieved with the option File Print Print button E hi OSI Channel button
186. wledge reading the standard with the OK button 7 Repeat the above for the high oxygen standard The peak at 600nm should be considerably lower at the high oxygen level Click the High Oxygen button and acknowledge with OK Aug 04 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc 99 Avantes website http www avantes com email Info avantes com antes 8 Calculate K and Ip values by clicking the Calculate button 9 Save the values for the calibration by clicking the Save Channel button Close the settings dialog by clicking the Close Form button 10 Enable the Oxygen measurements by selecting Application Oxygen Enable from the main menu After the application is enabled it is also possible to select Oxygen as a History Channel function 4 7 2 Enabling the application Vg AvaSoft 6 2 Full 2004 Avantes S N 020800451 File Setup view Application Help Stop p History wavelength Calibration Integration time m Color Measurement Process Control i Absolute Irradiance Measurement DEES Wavelength nim Enable Settings Enable the application by selecting Application Oxygen Enable from the main menu A checkmark will appear before the menu entry to show that the option is enabled After enabling you can select Oxygen as a function type in the Function Entry dialog of the History Application 4 7 3 Settin
187. wn in ve which the title for the printout can be ia entered In the next window the 5 printer settings can be changed e g a portrait or landscape printing oj printing quality etc After clicking l OK in the printer settings dialog the D Denun yoo HF Liila Se E Pages Libros L Popeetomsat Lene Lay Ahia cL epee FT Pogererrcept Laauiemateech tiiit Meter Fle AANTTSOO DH Anpi W0 Smoothing l Spine Ch 18 AVASOFT 6 2 for AvaSpec 102 256 1024 2048 Manual doc Aug 04 Avantes website http www avantes com email Info avantes com antes graph will be printed and the original graph colors will be restored on the monitor 3 1 9 File Menu Black and White printer The default setting in AvaSoft is to print the spectra in the same color as they appear on the monitor However if a color printer is not available the menu option Black and White printer can be enabled If this option is enabled different line styles will be printed if more than one spectrum is displayed e g dash dash dot dot dash dot To enable this option click the menu option and a checkmark appears in front of it 3 1 10 File Menu Display Saved Graph This option requires that graphic files were saved earlier by using the option File Save Experiment After choosing this option a window shows all files in the current measure mode In the example at the right the measure mode is scope so the extension of the earlier saved spectra i
188. you accidentally connected the AvaSpec spectrometer to the USB port of your PC before installing AvaSoft the USB drivers cannot be found and the spectrometer cannot be found by the later installed software To rectify an incorrect installation follow these steps 1 Navigate to the Device Manager If you have Windows98 Action View e m g li select Start Settings Control Panel Double click the System icon Select the Device Manager tab For 3 Windows2000 systems right click on My Computer M TA select Properties select Hardware tab and click on E Computer E l Device Manager button W Data Acquisition Devices I Disk drives 2 Scroll down until you see Other devices Jim Display adapters 3 Under Other devices you will see USB device with a 3 DYDICD ROM drives large question mark hit the remove button or right click Jy Floppy disk controllers the USB device and select the uninstall option Floppy disk drives 4 A warning box will appear to ask if it is OK to remove the IDE ATA ATAPT controllers device click OK g Fevee 0 E 5 Unplug the AvaSpec spectrometer from your PC and a a a pelea Gevikes ZA Monitors reinstall the AvaSoft software 4 Network adapters 6 Now you can plug the AvaSpec spectrometer to the USB IF Ports COM amp LPT port again Don t forget to connect the AvaSpec to the agf Sound video and game controllers 12V power supply as well Windows will display the H System devices

AVASOFT for AvaSpec-102/256/1024/2048 version 6.2 USER`S

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