Hematocrit Centrifuge Model 410E User Manual
Contents
1. cells The hematocrit reading is the percentage of the volume of packed red cells in relation to the volume of whole blood The hematocrit tube is filled with anticoagulated whole blood one end sealed by heat or insertion into a sealing clay and then centrifuged Interpretation of the resulting Volume of Packed Red Cells VPRC may be used to detect the presence of anemia or polycythemia Glossary Hematocrit Centrifuge An instrument which uses centrifugation to measure the ratio of the volume of red blood cells to the total volume of blood G Force The measurement for samples undergoing the stress of acceleration in a centrifuge RCF Relative Centrifugal Force The centrifugal force that the samples undergo which relies on the speed of rotation N in RPM and the rotating radius R The rotating radius is measured from the center axis of the rotor to the extreme ends of the centrifuge tubes RCF Formula RCF 11 18 x R x N 1000 Intended use The Grafco Hematocrit Centrifuge is intended to provide laboratory tests for hematocrit values HCT in the fields of biochemistry microbic immunity genetics and blood separation as well as general clinic tests Replacement parts Replacement parts available for the Grafco 410E Hematocrit Centrifuge from your Graham Field authorized distributor Graham Field Item 410E 1 Replacement Carbon Brushes package of two 410E INS LAB RevB 11 4 IMPORTANT SAFETY GUIDELINES PL2. the front of the centrifuge to unlock the lid Open the centrifuge lid 410E INS LAB RevB 11 9 6 Carefully open the rotor lid and remove the specimens A WARNING DO NOT open the centrifuge lid until the rotor has completely stopped spinning Opening the lid before the rotor has stopped spinning could result in personal injury INTERPRETING RESULTS USING THE READING SCALE g ti ty centrifuge adjustable bar tube gt capillary tube holder a extreme end a leucocyte a demarcation red blood cells scale 1 Remove the capillary tubes from the rotor Take care not to let the blood drain from the tubes 2 Place the capillary tubes on the grooves of the tube holder carefully Line up the bottom red blood cell portion of the tube with the straight line at the bottom of the reading scale so that the start of the red blood cell portion lines up with this horizontal fixed line 3 Move the tube holder from left to right on the reader until the top of the leucocyte line matches up with the fixed diagonal line on the reader platform 4 Move the adjustable bar to meet the demarcation place where the leucocyte and red blood cells separate The diagonal line on the adjustable bar should be directly on top of the demarcation 410E INS LAB RevB 11 10 5 The percentage of HCT can then be read directly from the scale by reading the number the adjustable d
3. top of the centrifuge A NOTICE Store within temperature limitations listed in following SPECIFICATIONS section a Modei Grafco 410E Hematocrit Centrifuge CE marking Conforms to LVD 73 23 EEC safety standards Standard Complies with EC Electromagnetic Compatibility requirement of EN60601 1 2 EMI EMI filter is designed in conformity with 89 336 EEC EMC Directive is ELT TEL ah eae ee in with 89 336 EEC EMC Directive nn as fate fm SSCS LANA LE AAN Speed RPM Fixed 12 000 though fixed at 12 000 RPM motor is capable of 14 000 RPM Capillary tube size 1 1 1 2mm x 75 mm length Conditions for Temperature 50 F 104 F 10 C 40 C T rtati and Storage Humidity 40 90 410E INS LAB RevB 11 14 LIMITED WARRANTY Your Grafco Hematocrit Centrifuge product is guaranteed to be free from defects in materials and workmanship for one 1 year under normal use from the date of purchase This WARRANTY does not apply to any product damaged by misuse abuse neglect improper line voltage dropping fire water submersion or saturation The warranty is void if non authorized representatives service or dismantle the centrifuge The warranty is void if damage occurs as a result of the lid being opened when the rotor is spinning If a product is deemed to be under warranty GF Health Products Inc shall provide at its option 1 replacement of any defective part or product or 2 a credit of the ori
4. 8 6 Close the centrifuge lid completely Press the locking button on the side of the centrifuge to lock the lid 7 Turn the ON OFF switch to ON position Setting the Digital Timer e The Grafco Hematocrit Centrifuge is equipped with an LED digital display timer which allows the user to spin the load from 1 to 99 minutes e Press the triangle directly below the left side of the timer to change the spin time in increments of ten 10 minutes e Press the triangle directly below the right side of the timer to change the spin time in single minutes e For example if the desired spin time is twenty five minutes press the left arrow twice to display a 2 on the timer and press the right arrow five times to display a five on the timer Operating the Centrifuge A WARNING As a safety feature the centrifuge has a power shut off device to turn the power off automatically when the lid is opened during spinning This is a safety feature of the centrifuge DO NOT open the lid when the rotor is spinning 1 After the desired spin time is displayed on the timer press the START button to initiate rotor spinning 2 The timer will display the spin time remaining during operation When the spin cycle is complete the unit will slowly stop spinning 3 To accelerate the deceleration press the BRAKE SWITCH 4 Once the rotor has completely stopped spinning turn the ON OFF switch to OFF position 5 Press the unlocking button located on
5. EASE READ BEFORE USE The safety statements presented in this chapter refer to the basic safety information that the operator of the centrifuge shall pay attention to and abide by There are additional safety statements in other chapters or sections which may be the same as or similar to the following or specific to the operations A WARNING Indicates a potential hazard situation or unsafe practice that if not avoided could result in death or serious personal injury A CAUTION Indicates a potential hazard or unsafe practice that if not avoided could result in moderate or minor personal injury A NOTICE Indicates a potential hazard or unsafe practice that if not avoided could result in product or property damage Info Provides application recommendations or other useful information to ensure that you get the most from your product A WARNING Important Read and understand these instructions before installing or using the Grafco 410E Hematocrit Centrifuge If you do not understand any part of these instructions contact your medical professional or Graham Field authorized distributor for direction in the use of this product A WARNING For the safety of both the operator and service personnel take care when handling substances that are known to be toxic radioactive or contaminated with pathogenic microorganisms when using this centrifuge A WARNING If components are damaged or missing contact your Graham Field autho
6. Grafco A Graham Field Brand Hematocrit Centrifuge Model 410E User Manual Read this manual before operating the 410E Hematocrit Centrifuge Save this manual for future reference The most current version of this manual can be found online at www grahamfield com 410E INS LAB RevB11 CONTENTS INTRODUCTION EE NE Waa RAAKAA NANN 3 PRODUCT OVERVIEW rrnvernnvenovornnvnnnvennnvnnnnvnnnvnnnnvnnnnnnnnvnnnvennvennnvennnnansvnnnneene 3 GLOSSARY A 4 INTENDED USE Se 4 REPLACEMENT PARTS mmrernnvonovennnvnnnnvnnnvnnnnvnnnvnnnnennnnnnnvennnvennnennnvennnnensvnnnnvene 4 IMPORTANT SAFETY GUIDELINES PLEASE READ BEFORE USE 5 SE FUP srana aaa oneen nra inidscenudsanaccnsssvaacseedcbsinianSsnnsascavadaadsdsisastatentstaanadddsraun 6 UNPACKING mmrnvnnnvnnnnvnnnvennvvnnnnnnnvnnnnennnnnnnnvennnennnnnnnnennnnnnnnvnnnennnnnensvennnennsnennneene 6 CONTENTS 440440 nana anna na anane anana anane anana ne anana anana anana anana nana ananannnan 6 INSTALLATION keenavnnvnnnnennnvnnnvennnnvnnnnnnnvennnnnnavennnennnnnnnnnnnnnennnnnnneennnnennnnnnseennne 6 SPECIMEN COLLECTION rnnnnvennnvnnnnvnnnvennnvnnnvnnnnvennnennnvnnnnnnnvnnnnnennnnnnvennnennenennnen 7 MATERIALS NEEDED uvnnnvvnnvvnnnvnnnnvnnvvnnnvnnnnnnnnvennnennnvnnnnennnnvnnnvnnnsennnnensvennnenr 7 CAPILLARY TUBE SPECIFICATIONS unnnvnnnvennnvnnnnvnnvvnnnnvnnnnennvennsennnnnnnsennnee 7 TUBE PREPARATION menavennnvennvvnnvvnnnnennnvnnnvnnnnnnnnvennnvenanennvennnnens
7. INS LAB RevB 11 7 2 Create a sealing plug Insert the dry ends of the tubes into the sealing clay and withdraw them with a twisting motion OPERATION Balanced Loads A NOTICE Your centrifuge must contain a balanced load in order to work properly Spinning unbalanced loads may shorten the life of the device and produce unpredictable results Observe the following rules when loading the rotor e Opposing rotor grooves must be empty or loaded with equally weighted samples e If an odd number of samples is to be spun fill a tube with water to match the weight of the unpaired sample and place it across from the unpaired sample Loading the Rotor The Grafco Hematocrit Centrifuge can hold up to 24 capillary tubes of size 1 2mm or 1 5mm x 75mm 1 Open the centrifuge lid if centrifuge lid is locked press the unlocking button on front of centrifuge to unlock it 2 Turn the rotor lid knob counter clockwise to unlock the it Open the rotor lid 3 Carefully place the test tubes in the rotor grooves ensuring the capillary tubes touch the rotor s outer rubber gasket 4 Make sure the load is balanced by placing similar tubes across from one another This will keep the rotor in balance see previous section Balanced Loads 5 Close the rotor lid gently Turn the knob clockwise to lock it A WARNING Closing the rotor lid improperly may result in broken capillary tubes which could cause injury 410E INS LAB RevB11
8. TED WARRANTY csaa a Ea EA AAAA AEAEE 15 Graham Field and Grafco are registered trademarks of GF Health Products Inc Packaging warranties products and specifications are subject to change without notice GF Health Products Inc is not responsible for typographical errors 410E INS LAB RevB11 2 INTRODUCTION Your Grafco Hematocrit Centrifuge has been designed to be reliable safe and easy to use This manual contains important operation and safety information Carefully read and understand the contents of this manual before installing or using the Grafco Hematocrit Centrifuge Always install this centrifuge in accordance with local electrical parameters Product Overview timer LED lid digital display TT 4 a 1 locking button brake te da mm unlocking j button L ER ON OFF timer set button switch ten minute increments timer set button one minute increments spin start button 410E Hematocrit Centrifuge The Grafco Hematocrit Centrifuge shown above features a digital timer up to 99 minutes fixed speed brake control and a reading scale This unit spins loads at a fixed speed of 12 000 RPM and has a thermal cutout switch to prevent overheated operating temperature The Grafco Hematocrit Centrifuge can hold up to 24 capillary tubes of size 1 2mm or 1 5mm x 75mm 410E INS LAB RevB 11 3 Centrifugation of anticoagulated whole blood results in the packing of red blood
9. e A NOTICE In order to spin balanced loads the centrifuge must be level To this end confirm the rubber supports are in good condition before each use A NOTICE Contact your Graham Field authorized distributor to replace the carbon brushes yearly or as needed Cleaning Clean the equipment as described below after each use 1 Unplug the unit and disconnect the power cord from the unit 2 Clean the outer case rotor lid and rotor tube holder grooves with a moist clean cloth 3 Ensure the equipment is thoroughly dry Attach the product s electrical cord to the cord port located on the back of the centrifuge 4 Plug the centrifuge s power cord into a properly grounded 110V 60Hz electrical wall outlet A NOTICE DO NOT use benzine or paint thinner for cleaning Transportation A NOTICE When transporting the unit place in secure packaging preferably the original packaging material which will prevent the unit from tipping over A NOTICE Do not drop the centrifuge turn it upside down or subject it to vibration A NOTICE Transport within temperature limitations listed in following SPECIFICATIONS section 410E INS LAB RevB 11 13 Storage A NOTICE Do not place this equipment in a draft sunlight or near a piece of equipment which emits heat or electromagnetic conduction emission A NOTICE Disconnect power supply when the centrifuge is not in use A NOTICE DO NOT place other equipment or objects on
10. eshooting corrective actions A WARNING Do not disassemble or dismantle the centrifuge Can t turn 1 Blown fuse 1 Contact Graham Field centrifuge 2 Disconnected electrical authorized distributor to replace ON cord fuse 10A 3 Worn carbon brushes 2 Connect electrical cord 3 Contact Graham Field authorized distributor to replace carbon brushes Erratic Worn carbon brushes Contact Graham Field authorized speed distributor to replace carbon brushes Unbalanced rotor Balance rotor load Imbalance 1 Tubes are improperly 1 Balance load positioned unbalanced load 2 Screw rotor knob to lock lid 2 Rotor lid is not locked Broken test 1 Tubes are improperly 1 Balance load positioned unbalanced load 2 Place tubes against rubber 2 Tubes are not placed all the gasket on rotor way down 3 Ensure rotor lid is placed flat 3 Rotor lid is tightened against rotor incorrectly MAINTENANCE A WARNING Centrifuge disassembly or dismantling is limited to authorized service personnel only A WARNING Always use a properly grounded electrical outlet of correct voltage and current handling capacity A WARNING Do not immerse the centrifuge in or saturate it with water A NOTICE Do not lubricate the drive motor or linkage assembly 410E INS LAB RevB 11 12 A NOTICE Do not drop the centrifuge turn it upside down or subject it to vibration A NOTICE Clean the chamber and rotor as described below after each us
11. ginal selling price made to GF Health Products Inc s initial customer The warranty does not include any labor charges incurred in replacement part s installation or any associated freight or shipping charges to GF Health Products Inc The warranties contained herein contain all the representations and warranties with respect to the subject matter of this document and supersede all prior negotiations agreements and understandings with respect thereto The recipient of this document hereby acknowledges and represents that it has not relied on any representation assertion guarantee warranty collateral contract or other assurance except those set out in this document 410E INS LAB RevB 11 15 USA Corporate Headquarters GF Health Products Inc 2935 Northeast Parkway Atlanta Georgia 30360 telephone 770 368 4700 fax 770 368 2386 e GRAHAM FIELD www grahamfield com Made in Taiwan 2011 GF Health Products Inc
12. iagonal bar crosses at the right side of the reader Expected Value Normal hematocrit values will vary somewhat depending upon such factors as age sex size of red cells and altitude Generally accepted normal values with range of variation are Women 36 46 e Men 42 52 Limitations and Factors Affecting Results 1 Inadequate centrifugation either time or RPM will result in erroneously high reading 2 Ensure that the capillary tube is sealed properly to prevent red cell loss during centrifugation The rubber gasket encircling the interior of the rotor must be smooth and pliable This will aid in clay sealing plug retention and reduce breakage during centrifugation 3 Take the reading from the top of the clay sealing plug to the top of the column of VPRC 4 Hemolized blood due to over anticoagulation or improper finger stick can produce erroneous results 5 Hematocrit determinations are not accurate immediately following blood transfusions or blood loss DISASSEMBLY A WARNING Disassembly or dismantling of this centrifuge is limited to authorized service personnel only Dismantling or disassembly by non qualified personnel will result in the voiding of the unit s warranty Please contact your Graham Field authorized distributor or customer service representative to identify a qualified technician 410E INS LAB RevB 11 11 TROUBLESHOOTING A WARNING Unplug the centrifuge before performing troubl
13. netic conduction emission 410E INS LAB RevB 11 6 1 Place the centrifuge in a large open space with unencumbered air flow on a sturdy flat level countertop with sufficient clearance to open the lid completely 2 Attach the product s electrical cord to the cord port located on the back of the centrifuge 3 Plug the centrifuge s power cord into a properly grounded 110V 60Hz electrical wall outlet A NOTICE Locate the unit so that the centrifuge will not be accidentally pulled or dislodged from the countertop and so the cord will not be stepped on tripped over or otherwise subjected to damage or stress SPECIMEN COLLECTION Materials Needed Capillary tubes If non anticoagulated blood is used for the procedure we recommend the use of heparinized capillary tubes If blood is already anticoagulated we recommend the use of plain capillary tubes Clay sealant Centrifuge with rotor which will accept capillary tubes and attain at least 11 500 RPM Chart for determining hematocrit values Capillary Tube Specifications Capillary Tubes Heparinized 1 D 1 4mm 1 2mm wall 0 2mm 0 02mm Plain length 75mm Precalibrated 1 D 0 5mm 0 6mm O D 1 4mm 1 7mm Heparinized length 75mm Calibration line 60mm from one end Tube Preparation 1 Using 2 capillary tubes insert an end of each into the blood tilt slightly and allow the tubes to fill by capillary attraction to 2 3 3 4 full 410E
14. rized distributor immediately DO NOT use substitute parts A WARNING GF Health Products Inc assumes no responsibility for any damage or injury caused by improper installation or use of this product 410E INS LAB RevB 11 5 SETUP Unpacking 1 Check for obvious damage to the carton or its contents If damage is evident please notify the carrier and GF Health Products Inc 2 Open the product box carefully This may include cutting external packaging strips which surround the product shipping box Also for added safety when shipping the centrifuge may sometimes be packaged inside the outer shipping box meaning two boxes must be opened 3 Carefully remove the Hematocrit Centrifuge from the packaging including removing the plastic bag in which the centrifuge is placed for shipping purposes Do not drop the centrifuge Ensure that all of the equipment listed in Contents following is present 4 Keep the packing materials for transportation and or storage Contents Your Grafco Hematocrit Centrifuge contains Equipment Quantity Description supplied Er with 4106 Rotor with lid aa Detachable power cord Installation A NOTICE The operation of AC motor is affected by the surrounding power consumption which will result in a fluctuation of speed To assure an accurate RPM and its corresponding RCF do not place this equipment in draft sunlight or near a piece of equipment that emits heat and or electromag
15. vennsennnnnennvenene 7 OPERATION mmrvennvnnnnvnnnvnnnnvnnnnnnnnvnnnnvnnnnnnnennnnnnnnvennnvenavennnnennnnennnnnnnnennnnnnennnnnensvennen 8 BALANCED LOADS 1 eennvernnvennvnnnnvnnnnvnnnvnnnnvnnnnnnnvennnennevennnvnnnnvnnnvnnnnennnnnnsvennnnnr 8 LOADING THE ROTOR mrnnnvnnnvnnnnvnnnnvnnvvnnnvnnnnnnnnvnnnnennnvnnnnennnnvnnnvnnnnennnnensvennnenr 8 SETTING THE DIGITAL TIMER rernnennnvennnvnnnvnnnnvnnnnvnnvvnnnnvnnnnnnnvennnvnnnvennnvenner 9 OPERATING THE CENTRIFUGE rernnnnnnvennnvnnnvnnnnvnnnnvnnnennnnennnnennevnnsennnsennnvennne 9 INTERPRETING RESULTS USING THE READING SCALE cesses 10 EXPECTED VALUE marennnvennnvnnnvnnnnvnnnnnnnvnnnnnvnnvnnnnvennnennnnnnnnennnnnnsnennnnennnennneennne 11 LIMITATIONS AND FACTORS AFFECTING RESULTS rnnvnnnvennnvennnvnnsvennse 11 DISASSEMBLY ennvernnvonnnvnnnvennnvennvnnnnvennnnnnnvnnnnvnnnnnnnvennnnnnnnnnnnennnnnennvennsennsnnnnneenn 11 TROUBLESHOOTING mennnvnnovnnnnvnnnnvnnvennnnvnnnvnnnvennnnennvennnnnnanennnvnnnnnnnnennnnvennnnnnnene 12 MAINTENANCE ernovornvennnvnnnvennnvennvnnnnvennnvnnnvnnnnvnnnnennvnnnnnensvnnnnennnnnensvennsennsnnnnneenne 12 CLEANING ernvrnnnvnnnnvnnnvnnnvennnvennvennnennnnnnnavennnnnnnnnnnnennnnnnnvnnnnnennnnensvennsennsnnnnneene 13 TRANSPORTATION ennvennvvenovnnnnvnnnvennvennnnvnnnvnnsvennnnennvnnnnvennnnnnnennnnvennnensvennnnnn 13 STORAGE See ae 14 SPECIFICATIONS mrnnnnnnvnnnnvnnnvvnnvennnnvnnnnnnnvennnnennvennnvennnnnnnvennnvennvennnennnnnnnnnnnnnene 14 LIMI
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