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Pipette Calibration System Student User Manual

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1. Historage Elec Controls Leica BRDG Caution Never attempt to hold or grab any component of the machine to stop motion Microtome SOP American Optical BRDG Mechanical Controls Prepared by Bob Morrison STLCC Instrumentation Specialist May 2008 updated Doc 2015 for models at BRDG STLCC CPLS Monon 12142015 Page 1 Microtome American Optical Model 820 SN 70298 SSTLCO CPLSMomaon 121472015 Page 2 Optical Lube Schedule Page 3 Microtome Leica Model 20008 Page 4 Microtome General Setup and Operating Instructions 1 Prepare specimen in paraffin or desired mounting material 2 Before plugging in the device mount specimen and the knife and adjust per page 4 3 Plugin the device and set electronic controls per page 5 4 Release the cutting flywheel by moving the locking lever up page 3 5 Engage the flywheel motion by moving the lever on the flywheel clockwise cw page 3 6 Engage knife platform motion by rotating the platform lock lever clockwise significant force is required page 3 7 Cancel or stop using the electronic buttons ONLY Never attempt to hold or grab any component of the machine to stop motion page 5 STLCC CPLS Morton 1214015 re Microtome Mechanical Controls Major Flywheel Specimen Motion up down lock unlock rotate to move specimen above knife area Free Flywheel lever down to lock up to allow H
2. C Speciment Adjust see next slide Knife Adjustments see next slide Knife Mount Lock CW to clamp knife for motion and slicing Rate of Cut 0 200 mm sec speed of up down motion CW to increase STLCC CPLSMoraon 12142015 Page 6 otome Mechanical Controls Knife and Specimen S DO NOT PLUG IN DEVICE AT THIS TIME S1 Release to remove entire block if needed S2 Clamp specimen to block S3 Specimen side control lock I K2 Release Levers set angle of knife mount K3 Set height of knife screw CCW then slide bar snco ceswonson Release Levers set angle of knife mount Page7 itorange with Electronic Controls 1 Plug in device 2 Select what to monitor stroke feed used feed remaining 3 Reset to counters to zero 4 Increase decrease desired 6 Must hold down specimen slice thickness button to move knife forward to obtain slices Start Multiple cut strokes 7 Push both to cancel slicing Hotlink to Historange model LKB 2218 STiCC CPLS Morton 12142015 Microtome Other Protocols Notes Future home of other or more details protocols STLCC CPLS Morton 1211412015 Page9

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