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HiQ Flowmate® Dual Syringe Pump User Manual

1. Figure 30 Stepped Flow Syringe Note The minimum step Specifications screen with pop up keypad duration is 4 seconds 22 afl Stepped Flow Set up yringe A At least two or more stepping ITEM o0 BT stages should be used for LN e stepped flow to work properly Howrate 2 00 ciens Flowrate 3 6 o0 f mL min e Repeat the above steps to enter values for E eus flowrate 2 3 and 4 If these steps will not be Flowrate 4 100 ML used either enter zero or keep the existing values e Make sure that the units selected for flowrate appear next to each flowrate numerical display e Press BACK to return to the Stepped Flow Setup screen e Repeat the above steps if syringe B is going to be used in stepped flow mode Figure 31 Flowrate Setup screen of stepped flow mode FLEXCELL9 INTERNATIONAL CORPORATION PAGE 34 g FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLUID FLOW MODULE OPERATION STEPPED FLOW MODE 6 Stepped Flow Control by Duration or Dispense Withdraw Volume e On the Stepped Flow Setup screen press CTRL button under syringe A Figure 27 The Stepped Flow Control screen will appear Figure 32 Control by Volume iure ea e Press SELECT button in the Volume saes E OE section under syringe A to select flow P cif o 6 ei ull regimen control by volume The button will cif of o 04 5M n turn green if it is properly activated F m E L L L e din square next to
2. Duration e Use the pop up keypad to enter the seconds e Repeat the same procedure to enter values for MM minutes HH hours and DD days e Repeat the above steps if syringe B 1s going to be used in oscillatory flow mode e Press BACK button to return to the Oscillatory Flow Setup screen Figure 36 Time left display The duration of the oscillatory flow regimen is set in the Oscillatory Flow Parameters screen Figure 37 The Time left on the Oscillatory Flow Setup screen will display the set duration initially and subsequently show the remaining time for the flow regimen to end F Syringe A EXE if TAE nm Lem D in FLUID FLOW MODULE OPERATION OSCILLATORY FLOW MODE Oscillatory Flow Units Syringe B Micro L nr i cm ma mLimin fj mane juLimin IM ua rL min I pLimin Figure 38 Oscillatory Flow Units screen Syringe Specifications S yringe A Sy Vol 10 06 mL oY ID 57 ie Syringe B Sy ol 5 00 f mL oY ID cE Figure 39 Syringe Specifications screen for oscillatory flow Syringe Specifications Numeric keypad 10 00 Figure 40 Oscillatory Flow Syringe Specifications screen with pop up keypad FLEXCELL INTERNATIONAL CORPORATION PAGE 39 A FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE S FLU FLOW MODULE OPERATION OSCILLATORY FLOW MODE 7 Setup of Syringe Travel Direction e On the Oscillatory Flow Setup screen unde
3. HiQ Flowmate E Osc pvc atory _ How CE yy eee i A Dose Flow Calculator Pulsatile Flow Stepped Flow 11 19 2010 14 45 26 Figure 6 HiQ Flowmate main menu screen FLEXCELL INTERNATIONAL CORPORATION PAGE 12 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE e Inputs and Outputs HARDWARE OVERVIEW Each flow mode consists of several inputs variables that need to be entered by using the touch screen The units for the inputs should be set separately for each flow mode Different units for inputs can be used for syringe A and B Based on the function of the flow mode the number and type of input may vary for each flow mode After selecting the flow mode it is important to first setup the units and input syringe specifications before entering values for the other inputs Following is a description of all the inputs outputs and switches used in the HiQ Flowmate touch screen Touch Screen Switch Category Description Screens Control Switches syringe A syringe B Action Disp Mi vvitnd Action Used to turn ON OFF Syringe A and or Syringe B Switches will flash when turned ON Press the switch to activate Used to select the direction of syringe motion Press to activate Used to select the direction of syringe motion Press the switch to activate Indicators under each switch will display the current direction of syringe travel The highlighted switch indicates the
4. 8 C 260 6 1 2 HiQ Flowmate Applications Dispensing or withdrawal of fluid to or from a target based on volume or on time Continuous unidirectional flow through a target area Low flowrate infusions Combined flow profiles using different flow modes through a single target area Simultaneous use of different flow profiles through multiple target areas Fluid shear stress applications VVVVV WV FLEXCELL INTERNATIONAL CORPORATION PAGE 8 FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW Hardware Overview HiQ Flow mate Dual Syringe Pump Drive Unit H Rear Safety Sensor L Rear Thrust Plate P Dual Thrust Bar Lead Screw M Front Thrust Plate Q Front Bracket J Guide Bars N Thrust Bar Thumb Screw R Front Safety Sensor K Thumb Screw O Top Thrust Bar S Guide Block Lock Button Figure 3 HiQ Flowmate dual syringe pump drive unit Drive Unit e Linear Guide Block System Function The HiQ Flowmate consists of two separate drive systems to move two separate syringes Figure 3 Each drive system consists of a lead screw Fig 3 part I two guide bars Fig 3 part j a guide block and a guide block lock button Fig 3 part S When the lead screw is rotated by a microprocessor controlled stepping motor the guide block travels along the lead screw and guide bars linearly to push or pull the syringe plunger clamped to the guide block e Dynamics of The Linear Slider System The guide
5. D for dispense or W for withdraw e Repeat these steps to set the direction of travel for syringe B if it is being used in Pulsatile flow mode Figure 25 Flow regimen status and safety e Please make sure that syringes sensor status display on the setup screen have enough distance from the ends syringe sizes are accurate to travel in their respective directions 8 Flow Regimen Control e Prior to starting the flow regimen make sure that the safety sensors are all inactive There should be no red sensor indicators in the safety sensor section see Figure 25 yellow oval e Press RUN button green triangle to start syringe motion The syringe travel can be stopped by pressing the button with the red square 1 e the STOP button Figure 25 e Press PAUSE button button with two yellow lines to pause the syringe travel Figure 25 If syringe travel is paused press START button again to restart syringe travel The syringe will restart from the paused state and continue to dispense withdraw remaining amount of liquid 9 Termination of Flow Regimen e Once the syringes have stopped after completing a regimen press STOP button to reset the system e Turn OFF the respective syringes by pressing the syringe A or syringe B button Figure 25 The background color of the button should turn black when properly turned OFF FLEXCELL INTERNATIONAL CORPORATION PAGE 31 eg FLEXCELL INTERNATIONAL CORPORATION HIQFL
6. FS Calculator 2 Select syringes by pressing Syringe A or Syringe B buttons The buttons will turn red and continue to flash when selected Figure 8 Pulsatile BEGUN Sigesed i pped How 3 Setting Up Units e Press UNITS button on the screen The Constant Flow Units screen will appear Figure 9 Constant Flow e Select units for syringe volume internal Sri ica EEDE diameter dispense withdraw volume and MAA 500 METNEEI 150 00 ETT flowrate by pressing the labeled buttons FRE a ne The selected buttons will turn blue when Mess __0 50 Sy ERE oes properly activated Note Every time the MdB RB 48 A pump is turned on the units need to be reset G Im lim pe ke Senso E gt m m ON in ul Stopped Stopi Status Statul fT e Once all units have been set return to the E D D mL uL Constant Flow Setup screen by pressing pem i BACK button Check and confirm that the unite M Syringe Spass ETE selected units appear next to the volume and Figure 8 Constant Flow Setup screen flowrate Fl Rate inputs in the Constant Flow Setup screen 11 19 2010 4 Syringe Specifications Setup e Press SYRINGE SPECS button on the Constant Flow Setup screen Figure 8 FLEXCELL INTERNATIONAL CORPORATION PAGE 21 HIQ FLOWMATE FLEXCELL INTERNATIONAL CORPORATION FLUID FLOW MODULE OPERATION CONSTANT FLOW MODE e When the Syringe Specifications screen Figure 10 appears pr
7. To0lS e aR 41 Dose Duration Flowrate Calculator siiis contro aab eb vss toto tiU eertain EEN EEA E 41 Shear ouress Calcul O us asesusecxstx oti dp si ian SEM ccs RORA ESL Ou E T p SE DL ON Mae do Ea bbr cuu 42 NN EDAT OE aaa 44 TOUDE NOONE ETE OO DUO D OSEE 47 Appen se ieu irs eshvSE ua Pei Edu o Skid Na RUD QUIS O E ses SEp rA NR EEEO 50 SOCIO CAL ONE NR TIT TTE TT DD TED 52 Fluid Flow Specifications Option A u ccccccssccessscssseccssscesssecessecessecesseecessecesseeceseeesseecesseeeseesenseeens 54 Fluid Flow Specifications Option B sesenta 55 Fluid Flow Specifications Option C aastosmsuacetrenttsc Vent sun tSch Parti tubus rta Een Id Fui Vas ean DE FEE Dept SURE 56 FLEXCELL9 INTERNATIONAL CORPORATION PAGE 1 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE WARRANTY FLEXCELL INTERNATIONAL CORPORATION ONE YEAR LIMITED PARTS WARRANTY Flexcell HiQ Flowmate Dual Syringe Pump l FLEXCELL INTERNATIONAL CORPORATION warrants to the original purchaser customer all hardware components of the Flexcell HiQ Flowmate Dual Syringe Pump serial for a period of one year from the date of delivery to the purchaser customer to be free from manufacturing defects in workmanship or materials with the following exceptions terms and conditions a b ITEMS EXCLUDED FROM THE WARRANTY ARE Instruction manuals CDs DVDs A
8. above settings will flow 70 ethanol through each path of the valve channels twice 8 To remove ethanol from fluid channels in the valve and manifold flow PBS or Deionized water by following the guidelines from 3 to 7 You may use multiple cycles of PBS or deionized water to ensure proper removal of all residual ethanol Connect tubing from port C and D to a container with PBS or deionized water and make sure that the tubing is immersed in the liquid 9 If you plan to use a syringe manually to flow 7096 Ethanol you may use the same settings on the CONTINUOUS FLOW MODE as given in steps 5 6 and 7 10 First fill up a 5 mL or 10 mL syringe with 7096 ethanol Then connect tubing from the syringe to port A Connect tubing from port C and D to a container with PBS or deionized water and make sure that the tubing is immersed in the liquid 11 Once the syringe motion is started start dispensing 70 ethanol manually at a relatively slow flowrate Do not dispense ethanol rapidly as the pressure increase could damage the valve The CONTINUOUS FLOW MODE settings would change the path of ethanol flow through the valve You may continue dispensing ethanol until the end of syringe travel duration 12 Repeat the above step with PBS or deionized water 13 Repeat steps 10 and 11 for ports C D and B to sterilize the second valve and second flow path of the valve manifold FLEXCELL9 INTERNATIONAL CORPORATION PAGE 46 z FLEXCELL INTERNATIONAL
9. channel height and width are known The units for these parameters can be selected on the same screen Calculator Setup 1 Press the SHEAR STRESS CALCULATOR symbol button on the HiQ Flowmate main screen Figure 45 to be directed to the Shear Stress Calculator screen Figure 46 2 Setting Up Units e On the Shear Stress Calculator screen select units for viscosity flowrate and channel width and height by pressing the labeled buttons Figure 46 The selected buttons will turn blue when properly activated Note Every time the pump is turned on the units need to be reset by turning ON and OFF e Check and confirm that the selected units appear next to all numerical inputs on the screen HiQ Flowmate Constant Oscilator Flory EE Flow A Continuous e Dose rn Flow continucus Tol Calculator Sor A Pulsatile EH Flow m Sires pa Stepped Flow ih EG Flow I AE 1i gin 010 ZI M eg Figure 45 HiQ Flowmate main screen showing Shear stress calculator access button p Shear Stress Calculator Dyn viscosity 1L 0091 E E Flow Rate n ioo fj mL rnin Channel Vvidth vv LOG N um Channel Height H3 5o 5 N unm fa 0 0000 TEE UE UNITS Calculate i NP qn E Figure 4 46 Shear Stress Calculator Setup screen FLEXCELL9 INTERNATIONAL CORPORATION PAGE 42 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES 0 CALCULATION TOOLS 3 Calculator Operation e On the Shear S
10. direction of syringe travel at the start of the program syringe motion START switch Press to activate syringe travel syringe motion PAUSE switch Press to pause syringe travel Syringe motion STOP switch Press to stop syringe travel Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Constant Flow Pulsatile Flow Stepped Flow Oscillatory Flow Continuous Flow Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow FLEXCELL INTERNATIONAL CORPORATION PAGE 13 g FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW Touch Screen Switch Category Control Switches Volume Micro L nt Lf Flowrate Fy imL r J uLimin f uLmr nLimin M4 pLimin FLEXCELL INTERNATIONAL CORPORATION Used to select the step stages for stepped flow Press the numbered button to activate each step Numbered button will turn blue when activated Red buttons will flash when a syringe goes through the corresponding step Units for syringe volume can be selected by pressing the labeled buttons Buttons turn blue when activated To select another unit press the already selected button first to deactivate Units for syringe internal diameter can be selected by pressing the label
11. discolor the sensor surface and block light transmittance Please periodically inspect and check all sensors to confirm that all sensors are in good working condition Valves and Manifold Cleaning and Sterilization The HiQ Flowmate consists of a single valve unit that is comprised of two 3 way rocker isolation valves and a manifold The isolation valves consist of a PEEK Polyetheretherketone body FFPM Perfluoroelastomer seals and diaphragm The valves can be used with liquids or gases in the temperature range from 10 C to 40 C 50 F to 104 F The valve manifold is comprised of a Delrin Acetal Polymer body and stainless steel micro nozzles Both PEEK and FFPM have excellent stability to most acids alkalines and solvents see appendix X for chemical stability information Delrin is also stable to most acids and solvents Sterilization It is essential to sterilize the internal channels of the valve and manifold after use to prevent fouling and contamination problems Sterilization of the internal channels of the valve and valve manifold can be done by flowing 70 ethanol through the valve and manifold You may use the HiQ Flowmate or use a syringe manually to flow ethanol through the valve unit When the syringes are connected to respective ports of the valve manifold each syringe will have two separate paths through the valve to reach port C and D Both these paths need to be sterilized Follow the guidelines shown be
12. e Connect tubing from reservoir to port D of the valve manifold e Connect port A of the valve manifold to the tubing from the inlet of the target area e Connect port B of the valve manifold to the tubing from the outlet of the target area Only one valve unit that is configured to be used with syringe A is F4 supplied with the system A secondary valve unit that can be configured to b ci be used with syringe B is optional and can be ordered separately ds Reservoir Figure 34 HiQ Flowmate valve unit for oscillatory flow mode operation FLEXCELL INTERNATIONAL CORPORATION PAGE 37 g FLEXCELL INTERNATIONAL CORPORATION HIOQFLOWMATE FLU FLOW MODULE OPERATION OSCILLATORY FLOW MODE Flow Regimen Setup HiQ Flowmate Constant Oscilator Flow c Flow continuous io Continuous i 1 Dose 1 Press the OSCILLATORY FLOW symbol button on the HiQ Flowmate main screen Figure 35 to be directed to the Oscillatory Flow Setup screen Figure 36 2 Select syringes by pressing Syringe A or Syringe B buttons The buttons will turn red and continue to flash when selected Figure 36 Flow Pulsatile Flow FI m Steppec E J BELA 3 Setting Up Units EVECYERETI e Press SET PARAMETERS button on the Fi Calculator Oscillatory Flow Setup screen The Oscillatory Flow Oscillatory Flow Parameters screen will Syringe A Syringe B appear Figure 37 F Rate 0 50 mtfmin 0 25 mLfmin e Press UNITS
13. modifications of the equipment can be made which may result in the equipment failing to meet the limits of the FCC Rules 5 The equipment must be maintained in a satisfactory state of repair 6 Shielded cables must be used with this equipment to ensure compliance with the FCC Rules If the unit does interfere with radio or television reception which can be tested by turning the unit off and on the user is encouraged to try the following suggestions to correct the interference Reorient the antenna of the receiver Relocate the equipment to increase the distance between the unit and the radio TV Connect the unit to an outlet on a different circuit from the receiver Consult with the dealer or an experienced radio TV technician for advice on ways to reduce interference ud adi dde NOTICE The information in this document is subject to change without notice Flexcell International assumes no responsibility for any errors that may appear in this guide This manual is believed to be complete and accurate at the time of publication In no event shall Flexcell International Corporation be liable for incidental or consequential damages in connection with or arising from the use of the manual Customers must contact Flexcell International Corporation or an authorized distributor before opening the case on the HiQ Flowmate Dual Syringe Pump FLEXCELL9 INTERNATIONAL CORPORATION PAGE 4 FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMAT
14. not store the HiQ Flowmate unit at temperatures above 140 F 60 C or below 14 F 10 C e Do not operate the HiQ Flowmate unit at temperatures above 104 F 40 C or below 40 F 4 C FLEXCELL INTERNATIONAL CORPORATION PAGE 5 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES o QUICK START GUIDE e Do not store or operate the HiQ Flowmate unit at relative humidities exceeding 85 or below 20 Always use HiQ Flowmate in non condensing environment Do not place or operate the unit on wet surfaces or near liquid or oil spills Make sure that the unit is properly grounded through the A C wall outlet Do not open the cover of the unit or touch circuitry inside If damage 1s suspected to the unit do not operate the unit Contact Flexcell International to obtain advice e Use the unit in the recommended orientation Orienting the unit in any other way would damage the touch screen and other mechanical parts 1n the device e Read all warning labels on the unit to ensure proper usage ste emt Flot np HQ pul psyrin I S jio puc M anual Figure 1 HiQ Flowmate components FLEXCELL INTERNATIONAL CORPORATION PAGE 6 eg FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES INTRODUCTION TO THE HIQ FLOWMATE Introduction to the HiO Flowmate The HiQ Flowmate has been designed to provide researchers with a complete dual syringe pump system that has a multitude of functions to carry out
15. press the square next eser re un De E to the numerical input besides the Sy Vol ee sum dR uo and Sy ID under syringe A and enter the volume of the syringe and internal diameter of the syringe respectively using the pop up key pad Figure 24 If the units have been setup properly the selected units will appear next to the Sy Vol and Sy ID Figure 22 Pulsatile Flow Units screen numerical displays Refer to manufacturer specifications or manually measure using a Syringe Specifications caliper to determine the internal diameter of the syringe S yringe A e Repeat the above step for syringe B if it is Bani oli ii being used in this flow mode ONE 14 57 MOS e Press BACK button to return to the Pulsatile f Flow Parameters screen UETES ELS 5 Setting up Pulsatile Flow Profile Parameters SONS 10 30 MS e In the Pulsatile Flow Parameters screen Byrnge EAE MUI sac RN Figure 21 press the square next to flowrate Fl rate numerical display under syringe A Figure 23 Syringe Specification screen for pulsatile flow FLEXCELL9 INTERNATIONAL CORPORATION PAGE 29 g FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLU FLOW MODULE OPERATION PULSATILE FLOW MODE e Use the pop up keypad to enter the desired flowrate e Press the squares next to the cycle time Numeric keypad Cyc time minutes M and seconds S numerical displays under syringe A and use the pop up keypad to enter the desired
16. the volume 1 numerical Volume EN NE 05 oon e Use the pop up keypad to enter a value for PE rs T rmm y volume 1 the volume of liquid dispensed or egeo gy uon d withdrawn during stepping stage 1 EI 200 foe ooo Jl e Repeat the above step to enter values for volume 2 3 and 4 If these stepping stages will not be used either enter zero or keep the existing values e Repeat the above steps if syringe B 1s going to be used in stepped flow mode Figure 32 Stepped flow control screen Control by Duration e On the Stepped Flow Control screen press SELECT button in the Duration section under syringe A to select flow regimen control by time The button will turn green if it is properly activated Figure 32 e Press square next to the seconds SS numerical input of duration 1 e Use the pop up keypad to enter a value for seconds in duration 1 2 e Repeat the above steps to enter values for oor minutes MM and hours HH in duration 1 E 1 gt cycle These inputs are the duration for stepping Mir Action stage 1 e Repeat above steps to enter hours minutes and seconds into duration 2 3 and 4 If these stepping stages will not be used either enter zero or keep the existing values Figure 33 Selecting steps and setting number of cycles on the Stepped flow setup Note You should use at least g two stepping stages to use the stepped flow mode successfully 7 Selecting the Stepping Stages e On the Steppe
17. to go to Oscillatory Flow Units IERTARE screen Figure 38 Rydur 0 50 MM ss O 3 0 miss e Under syringe A select units for syringe volume internal diameter and flowrate by pressing the labeled buttons Figure 38 The Lp ERAN Gd selected buttons will turn blue when properly G Oi m FEST NI qz B In E Time left 00 00 00 00 OO 00 00 00 DD HH MM 5 activated Note Every time the pump is as Status SUNT is Status SUR turned on the units need to be reset by p Help fi Menu turning ON and OFF Figure 36 Oscillatory Flow Setup screen e Units for syringe B may be selected if it will be used in this mode Syringe A ovringe B 2 Once all units have been set return to the Fl rate osdi raLf min o 25 jf E Oscillatory Flow Parameters screen by rw dur nf en os OB GIT Ms pressing BACK button Check and confirm 2a that the selected units appear next to the rr maj mend 5 and Ems flowrate Fl rate numerical input in the RSS Oscillatory Flow Parameters screen Figure oM cili solli sc olf i so off 37 A NNMERO Oscillatory Flow Parameters 4 Syringe Specifications Setup aT e Press SYR SPECS button on the Oscillatory SA MIET N A Flow Parameters screen Figure 37 e When the Syringe Specifications screen Figure 39 appears press the square next to the numerical input besides the Sy Vol and Sy ID under syringe A and enter the volume of the syringe and internal diameter of the s
18. to show the starting syringe directions The left directional indicators under the dispense and withdrawal buttons show the current travel direction of both syringes These indicators will flash when its respective direction 1s being run Syringe Control Status Bar The syringe control status bar in the lower center of the Continuous Flow screen displays the current syringe control status Figure 18 When the syringes are not in use it will display Status It will display Started when a flow regimen is started Paused when a flow regimen is paused and Stopped when a flow regimen is stopped Valve Control Display Two 3 way valves are used in the Continuous flow mode These valves switch on and off with each change in syringe travel direction to maintain unidirectional liquid flow through a target area The flow status of the valves is displayed in the valve control display area of the Continuous Flow screen Figure 16 yellow oval Letters A and B denotes valves 1 and 2 respectively In Continuous flow mode valve A is connected to syringe A while valve B is connected to syringe B When syringe A and syringe B are in dispense and withdrawal mode respectively the valve control area will display Flow out next to valve A and Flow in next to valve B The display will read vice versa when syringe A is in withdrawal mode and syringe B is in dispense mode When no action is selected for the syringes no travel direction is selected val
19. without having to stop the flow regimen and fill up syringes with liquid to maintain continuous flow Tubing Connection to Valve Manifold Two 3 way valves are used in the Continuous flow mode Figure 13 These valves switch on and off with each change in syringe travel direction to maintain unidirectional liquid flow through a target area It 1s important to connect tubing with the valve manifold accurately to maintain proper unidirectional flow e Connect tubing from syringe A to port A of the valve manifold e Connect tubing from syringe B to port B of the valve manifold e Connect port C of the valve manifold to the tubing from the outlet of the target area e Connect port D of the valve manifold to the tubing from the inlet of the target area FLEXCELL9 INTERNATIONAL CORPORATION PAGE 24 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLU FLOW MODULE OPERATION CONTINUOUS FLOW MODE Syringe Syringe A f Figure 13 HiQ Flowmate valve manifold for continuous flow mode Flow Regimen Setup Flowmate 1 Press the CONTINUOUS FLOW symbol button on the HiQ Flowmate main screen Figure 14 to be directed to the Continuous Flow Setup screen Figure 15 con conseant a Oscillatory Flow Flow Continuous Dose ees Flow mm Calculator er Aw Pulsatile oo cow Si ened meal poed o How Ow 11 19 2010 2 Units and Syringe Specifications e To setup units and enter syringe specificatio
20. CORPORATION HIQFLOWMATE8 APPENDIX Troubleshooting PROBLEM CAUSE CORRECTION HiQ Flowmate unit does not gt HiQ Flowmate may not Ensure plug is tightly turn ON be plugged in or plug may pushed into the outlet be loose PLC battery has expired Contact Flexcell Building fuse blown or gt Contact supervisor or tripped building manager Power outage Contact the local electric company HiQ Flowmate touchscreen gt HMI hardware problem Contact Flexcell stops working properly HiQ Flowmate unit turns Safety timeout software has Touch the HMI touchscreen OFF during operation been activated to keep working The HiQ Flowmate has Remove the HiQ d in an environment Flowmate from the not within the environment and return to specifications listed in the room temperature and Appendix humidity and contact Flexcell gt The fan inside of the unit gt Shut down the HiQ stops working and the Flowmate and contact hardware overheated Flexcell HiQ FLOWMATE FLOW MODES DO NOT START PROPERLY The HiQ Flowmate syringe The Syringe A or the gt Press the Syringe A or shaft does not move when the Syringe B button for the the Syringe B button START button is activated active syringe shaft 1s not Next press the Stop activated on the current button to reset any active flow mode main screen mode and then press Start A previous regimen has not gt Press t
21. E QUICK START GUIDE Quick Start Guide Unpacking and Inspection e The unit should be inspected thoroughly for any shipping damage or missing components e The HiQ Flowmate package Figure 1 includes o Syringe pump unit Power cord Instruction manual Two plastic 1 mL and 10 mL syringes Stylus and holder Accessories e Extended thrust bar for clamping a single large syringe up to 140 mL e Dual extended thrust bar for clamping two large syringes up to 140 mL e Stainless steel blunt needle with luer Polypropylene hub 16 gauge x length Pack of 25 refer to table below e Stainless steel blunt needle with luer Polypropylene hub 24 Gauge x 1 length Pack of 25 refer to table below e Tubing and micro nozzle fitting kit for valve manifold refer to table below e Secondary valve unit for oscillatory flow mode operation with drive shaft B Optional O UO Valve manifold micro nozzle fittings Lee Company Connector OD P N for Micro Tubing ID Dolenasvisue ub mm Nozzle mm d Connector Pack of 25 0 020 0 51 INZA3100914K 0 02 0 51 24 Gauge x 1 0 065 1 65 INZA3362597D 1 16 1 59 16 Gauge x 1 2 Valve Manifold Micro Nozzle Size Option SS Needle with Luer e Please notify Flexcell International immediately if there are any discrepancies or missing components Installation e Insert the power plug into a standard A C wall outlet 100 230V AC 50 60 Hz Equipment Precautions e Do
22. HiQ Flowmate Dual Syringe Pump User Manual E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES gt TABLE OF CONTENTS Table of Contents Stile fee 1 a ec 1s o E E 5 Unpacking and VS CCE OD serrr a EEEE NEE RE EEEE REN 5 Saa e EE 5 Eguipmett P recdiltlOll So sepoiet TA Pietro MN orca UU RUNS 5 Introduction to the HiQ Flowmates ccccccsssssccccccssssssccccccessscsccccccsseessccccscsesessecsssesessees 7 HiQ Flowmate Bp SS NORTE 8 HiO Flowmate Configurations sesine ipen E EE E A 8 PLO TFlowmato ADDCSLIODS ris siescaustistoncasncanasbnteveatintusulinat shnsiauagveisbadonsatneaghsantoneavaiatanttaaventsgsioponiaden s 8 Hardware OVE IOW er EE nen ee ee en eer ee ee 9 HiQ Flowmate Dual Syringe Pump Drive Unit sse nennen 9 Biz Bad RR se aeasaawutudecasaectene E E E 9 Mounting Syringes on the HiQ FlowmateS sess 10 L hw c RI RR ET TT TT STET 10 Wale Way ciao MING RET 11 Introduction to Touch SCIEGN ccccccssccsssscessecesssecsssecessecessecesseecsseecesseecsssecsssecesseecsseecesseessecesatecsas 12 Fluid Flow Module Operation cccccssssssssssssssseececececccccccececcecsssseeseseeeecececeecceceeeeesseseess 21 Constan OW MOC ccs toes see th ui matttenia A E encE SNR ENORMES 21 OTTO EON M Un 24 PSG OW 28 BE FOV NER RTT 33 s ellizie Ue ERU T T 37 Calculation
23. L 38 40 0 56 uL min 83 38 mL min 56 70 mL min FLEXCELL9 INTERNATIONAL CORPORATION PAGE 54 m FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES gt SPECIFICATIONS Fluid Flow Specifications Option B Flow Modes Constant Continuous Stepped and Oscillatory Flow Flow Mode Pulsatile Syringe Volume ID mm Min Flowrate Max Flowrate Max Flowrate FLEXCELL INTERNATIONAL CORPORATION PAGE55 m FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES gt SPECIFICATIONS Fluid Flow Specifications Option C Flow Modes Constant Continuous Stepped and Oscillatory Flow Flow Mode Pulsatile Syringe ID mm Min Flowrate Max Flowrate Max Flowrate Volume T 0 ul zm XU 00 uL 250 uL Iml 26 70 83 98 nL min 125 98 mL min 85 66 mL min 140 mL 38 40 173 72 nL min 260 58 mL min 177 19 mL min FLEXCELL INTERNATIONAL CORPORATION PAGE 56
24. NTERNATIONAL CORPORATION PAGE 28 7 FLEXCELL9 INTERNATIONAL CORPORATION HIQ FLOWMATE FLUID FLOW MODULE OPERATION PULSATILE FLOW MODE and continue to flash when selected Figure 20 3 Setting Up Units Pulsatile Flow Parameters e Press SET PARAMETERS button on the Syringe A Syringe B Pulsatile Flow Setup screen The Pulsatile GOMA Ema 10 00 Miner Flow Parameters screen will appear Figure eA M 58 co MES B 5 0 Mes 2L s dur O8 OB SEM CB c e Press UNITS to go to Pulsatile Flow Units screen Figure 22 e Select units for syringe volume internal diameter dispense withdraw volume and flowrate by pressing the labeled buttons Figure 22 The selected buttons will turn blue when properly activated Note Every Figure 21 Pulsatile Flow Parameters screen time the pump is turned on the units need to be reset by turning ON and OFF e You may select units for both syringes or any one of the syringes e Once all units have been set return to the Pulsatile Flow Parameters screen by pressing BACK button Check and confirm that the selected units appear next to the volume Vol and flowrate Fl Rate inputs in the Pulsatile Flow Parameters screen 4 Syringe Specifications Setup l l l Pulsatile Flow Units e Press SYR SPECS button on the Pulsatile ainge ens Flow Parameters screen Figure 21 SEE Microl finr DL MicroL fmt C f e When the Syringe Specifications screen A lcm dio oom Hem Hin jn Figure 23 appears
25. OWMATE FLU FLOW MODULE OPERATION PULSATILE FLOW MODE e Use the MENU button to return to the main menu if you need to select another flow mode e Before turning off the system use the MENU button to go back to the main menu If the syringe A and or syringe B is not turned OFF in this mode X prior to exiting this mode you will not be able to use the same syringe Q 9 in any other flow mode Help Screen Press HELP button to access the help information on pulsatile flow mode The help screens provide brief guidelines on how to setup a flow regimen in the pulsatile flow mode FLEXCELL9 INTERNATIONAL CORPORATION PAGE 32 g FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE S FLUID FLOW MODULE OPERATION STEPPED FLOW MODE Stepped Flow Stepped flow mode can be used to generate a fluid flow that increases or decreases in a stepwise pattern A total of four stepping stages can be programmed with independent flowrates Each step stage can be individually controlled based on either time or dispense withdrawal volume The travel of the syringes 1s controlled independently allowing the user to conduct multiple experiments simultaneously User needs to set the fluid flowrate for each step duration or dispense withdraw volume for each step number of stepping cycles and select stepping stages for the flow regimen using the touch screen Flow Regimen Setup 1 Press the STEPPED FLOW symbol button on the HiQ Flowmate main screen F i
26. am During the oscillatory flow mode Figure 49 the syringe movement is constantly forward while in the dispense setting and constantly reverse while in the withdrawal setting The oscillatory motion is controlled by two actuating valves inside of the valve unit Diagram A represents the syringe forward motion dispense and counter clockwise flow inside the target Diagram B represents syringe forward motion dispense and clockwise flow inside the target created by the valve unit actuating to reverse the flow FLEXCELL9 INTERNATIONAL CORPORATION PAGE 51 7 FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE SPECIFICATIONS Specifications 0 02 0 05 0 5 uL 140 mL Constant Continuous Pulsatile Stepped Oscillatory Option A Option B Option C L377 11 77 IU 83 38 260 57 260 57 0 72 Stepper motor with low vibration and smooth drive function High speed pulse control with microstepping Option A Option B Option C 1 10 1 10 1 100 50 000 100 000 1 000 000 Option A Option B Option C 150K 150K 1500K Option A Option B Option C 0 48 1 5 0 15 12 225 DS 115 Lbs 11 Lbs 11 Lbs FLEXCELL9 INTERNATIONAL CORPORATION PAGE 52 f FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE SPECIFICATIONS Valve Unit Valve Type 3 Way miniature rocker isolation valve Body PEEK Seals and Diaphragm FFPM AJETET See Appendix for chemical stability Differential Pressure 1 Bar 100 kPa Valve Manifold Mater
27. block is coupled to the lead screw by a lock button Fig 3 part S The lock button is spring loaded and can be disengaged from the lead screw by pressing it towards the guide block When disengaged the guide block can be moved freely along the lead FLEXCELL9 INTERNATIONAL CORPORATION PAGE 9 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW screw and positioned anywhere along the lead screw This function helps when positioning the syringe plunger anywhere in the syringe at the start of the flow regimen Mounting Syringes on the HiQ Flowmate The syringes are clamped onto the pump at three positions The syringe plunger is clamped to the guide block using the thrust plates Fig 3 parts L amp M while the flange of the syringe barrel 1s clamped similarly to the front block The syringe barrel 1s also clamped down to the front block from the top using a thrust bar Fig 3 part O Follow the guidelines below to accurately mount the syringe on to the pump 1 2 3 4 5 6 Loosen the thumb screws Fig 3 part K in the guide block and front bracket connected to thrust plates Select the appropriate thrust bar to suit the syringe size For syringes larger than 50 mL you may use the extended thrust bar or dual thrust bar Place the barrel of the syringe in the front bracket slot and adjust the guide block position based on the location of the syringe plunger so that the plunger plate can be easily s
28. ccessories DURING THE WARRANTY PERIOD the purchaser customer must notify Flexcell of any warranty claim in writing by telephone fax transmission or email identifying each defective part or specifically describe the exact problem no later than the last day the warranty is in effect FLEXCELL AGREES to correct any defect in workmanship or material and supply new or rebuilt parts in exchange for defective parts upon completion and submission by purchaser customer of a printed Parts Return Authorization form furnished by Flexcell Parts must be properly packed in original container and shipped to our factory service center or distributor with all shipping costs prepaid if the unit is out of warranty coverage If the original shipping box is not available Flexcell will send the required protective shipping container Flexcell will recommend the insurance value for parts or equipment to be shipped Return carrier shipping costs will be paid by Flexcell from the service center The purchaser customer is solely responsible for payment of custom fees taxes holding fees or value added taxes THIS LIMITED WARRANTY only covers failures due to defects in materials or workmanship which occur during normal use It does not cover damage which occurs in shipment or failures of original equipment due to products identified as add ons not manufactured by Flexcell International Corporation or its distributors nor does this limited warranty cover damages or fai
29. ce with a lint free cloth once a month or periodically as necessary Do not use strong detergent bleach acids or solvents on the touch screen surface as it can harm and discolor the screen Replacing the Battery The battery service life of the touch screen is approximately five years at 25 C 779F The service life of the battery will be shortened if the battery 1s used at higher temperatures The battery must be replaced after backing up the data FLEXCELL9 INTERNATIONAL CORPORATION PAGE 44 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE MAINTENANCE Safety Sensor Cleaning and Testing The photomicro safety sensor installed in the HiQ Flowmate prevents the guide blocks from colliding with the two end blocks Therefore it is important to maintain the photomicrosensors in good working condition to prevent any damage to the guide blocks The sensors operate using infrared light In order to prevent any blockage of light passing through the sensor surface the sensor surfaces should be kept free from dust lint and other materials that would block light transmittance You should periodically clean sensor surface with a soft clean cloth to remove dust or other matter You may partially moisten the cloth but do not use a dripping wet cloth to clean the sensor as water 1f seeped through could damage the underline circuitry Do not use any acids alkaline or solvents to wipe off the sensor surface Use of harsh chemicals would
30. cell and molecular biology experiments under fluid flow conditions The HiQ Flowmate provides general fluid pumping capabilities as wells as advanced fluid flow modes i e oscillatory and pulsatile flow The HiQ Flowmate has a large touch screen interface Figure 2 part A for quick access to different functions and useful aids to set up the various fluid flow modes A Touch Screen C Rear Bracket E Front Bracket G Valve Manifold B Drive Unit Base D Guide Block F Valve Enclosure Figure 2 Schematic of HiQ Flowmate Dual Syringe Pump FLEXCELL9 INTERNATIONAL CORPORATION PAGE 7 eg FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES o INTRODUCIION TO THE HIQ FLOWMATE HiQ Flowmate Features The HiQ Flowmate is designed to operate as a dual syringe pump This device utilizes five separate modules constant flow continuous flow ramped flow ocillatory flow and pulsatile flow The syringes are driven by two separate guide blocks that travel on lead screws The lead screw rotation is controlled by two independent microprocessor controlled microstepping motors The drive system has been designed to provide an accurate smooth and vibration free syringe motion Advanced microstepping techniques with smooth drive function have been employed to create continuous non pulsating fluid flow Due to the high resolution of the stepping motor drivers the HiQ Flowmate is able to reach a broad range of flowrates from picolit
31. conds e Repeat the same procedure to enter values for MM minutes HH hours and DD days e Repeat the above steps if syringe B is being used in Pulsatile flow mode e Press BACK button to return to Pulsatile Flow setup screen Figure 20 FLEXCELL INTERNATIONAL CORPORATION PAGE 30 A FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLU FLOW MODULE OPERATION PULSATILE FLOW MODE Duration and Time Left Display The duration of the pulsatile flow regimen is displayed on the Pulsatile Flow screen Figure 16 next to Duration in seconds SS hours HH minutes MM and seconds SS This duration of the flow regimen is calculated from the values entered in the duration numerical input if flow regimen is controlled by duration or from the value entered in the volume numerical input if flow regimen is controlled by dispense or withdraw volume on the Pulsatile Flow Parameters screen The Time left on the Pulsatile Flow screen will initially display the calculated duration and subsequently show the remaining time for the flow regimen to end 7 Setup of Syringe Travel Direction Pulsatile Flow e On the Pulsatile Flow setup screen under Syringe A syringe A press DISP to set the syringe A to BG NN s s STOO ee UNE TL dispense liquid or press WITHD to set syringe A to withdraw liquid into the syringe Figure 25 The set syringe direction will be displayed in the direction status box next to the status bar either
32. ctions on how to clean the sensor surface Safety Sensor Display The status of the safety sensors are displayed at the center of the setup screen for each flow mode Figure 4 top left If the sensors are activated the display square color will change from green to red Figure 4 bottom left Sensor alert messages will pop up indicating the activated sensor In that case move the guide block sufficiently away from the sensor and restart the flow regimen FLEXCELL9 INTERNATIONAL CORPORATION PAGE 10 FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW Figure 4 HiQ Flowmate sensor function showing photomicrosensor status display on flow mode screens top left sensor status display with syringe A front sensor in activated state bottom left and sensor alert pop up message right for syringe A front sensor Valve Unit and Fittings Function of Valve Unit The built in valve unit in the HiQ Flowmate is used to control the direction of fluid flow The valve unit consists of two 3 way valves a manifold and an enclosure The valves comprised of minimum internal volume and are manufactured with biocompatible materials to suit for biological applications The valves are mounted to the manifold which utilizes internal fluid channels to control the direction of fluid flow The fluid flows in and out of the manifold through four stainless steel micronozzles 4 ports fitted into the manifold It is of great importa
33. d Flow Setup screen in the Steps section Figure 33 red oval press 1 2 3 and 4 buttons to select stepping stages for the stepped flow regimen e The buttons will turn blue when properly activated The red indicators below each button will flash when the corresponding stage is being run FLEXCELL9 INTERNATIONAL CORPORATION PAGE 35 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLUID FLOW MODULE OPERATION STEPPED FLOW MODE 8 Setting the Number of Cycles e On the Stepped Flow Setup screen under syringe A press the square next to Cycle numerical input Figure 33 yellow oval e Use the pop up keypad to enter the number of cycles for the stepped flow regimen The cycle number should be equal to or greater than 1 e The set cycle number will appear next to the cycle number numerical input immediately after the flow regimen is started and will count down the number of cycles until the end of the following regimen 9 Setting Up the Syringe Travel Direction e On the Stepped Flow Setup screen under syringe A press DISP to set the syringe A to dispense liquid or press WITHD to set syringe A to withdraw liquid into the syringe Figure 33 The set syringe direction will be displayed in the direction status box next to the status bar either D for dispense or W for withdraw e Repeat these steps to set the direction of travel for syringe B if it is being used in stepped flow mode Please make sure
34. e syringe will restart from the paused state and continue to travel through the remaining time Figure 41 Flow regimen control and safety sensor display on Oscillatory setup screen 9 Termination of Flow Regimen e Once the syringes have stopped after completing a regimen press STOP button to reset the system e Turn OFF the respective syringes by pressing syringe A or syringe B button Figure 41 The background color of the button should turn black when properly turned OFF e Use the MENU button to return to the main menu if another flow mode needs to be selected e Before turning off the system use the MENU button to go back to the main menu If the syringe A and or syringe B is not turned OFF in this mode prior to exiting this mode you will not be able to use the same syringe in any other flow mode Help Screen Press HELP button to access the help information on oscillatory flow mode The help screens provide brief guidelines on how to setup a flow regimen in the oscillatory flow mode FLEXCELL INTERNATIONAL CORPORATION PAGE 40 FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE CALCULATION TOOLS Calculation Tools Dose Duration Flowrate Calculator This tool can be used to calculate dose duration or a flowrate for injection of a single drug or multiple Flowmate drugs to a target location or an animal The weight of the animal and the concentration of the drug have to be known Con con
35. ed buttons Buttons turn blue when activated To select another unit press the already selected button first to deactivate Units for dispense withdrawal volume can be selected by pressing the labeled buttons Buttons turn blue when activated To select another unit press the already selected button first to deactivate Units for flowrate can be selected by pressing the labeled buttons Buttons turn blue when activated To select another unit press the already selected button first to deactivate Stepped Flow Units screens of Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Units screens of Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Units screens of Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Units screens of Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow PAGE 14 eg FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW Touch Screen Switch Category Numerical Inputs TRA oor 1 m Volume numerical input 1s used to input dispense or withdrawal volume Press the square next to display and a key pad will pop up Type the desired value and press enter Selected units will be displayed in front of the numerical input Another volume numerical input as described above In addition to the volume input the red button below the numerical input is used to select the pul
36. er to milliliter scale The HiQ Flowmate utilizes a valve unit to create continuous and oscillatory flow The valve unit is built into the pump and is controlled by the HiQ Flowmate software The HiQ Flowmate utilizes a touch screen interface and advanced software for operation The large touch screen with user friendly icons and buttons eliminates the need to use an external computer for control The HiQ Flowmate application software provides help screens to aid in creating flow regimens as well as information on most common syringe dimensions There are also calculation tools such as the Dose Duration Flowrate calculator and Shear Stress calculator to ease set up of flow regimens The flow mode screens display real time flow regimen status either in time or volume scales Additionally the use of a dual independent drive system in the HiQ Flowmate permits the user to program different flow modes in the separate syringes except when the continuous flow mode is used The use of a dual independent drive system enables users to utilize HiQ Flowmate for multiple tasks simultaneously HiQ Flowmate Configurations The table below lists the available configurations and their flowrate ranges Configurations A and B include the drive system with smooth drive functions Configuration C does not have smooth drive function Configuration Maximum Flowrate with 140 mL Minimum Flowrate with 0 5 uL Syringe mL min Syringe pL min A 83 4 3 8 B 260 6 11
37. ess the square next to the numerical input besides the Sy Vol and Sy ID under syringe A and enter the volume of the syringe and internal diameter of the syringe respectively using the pop up key pad Figure 11 If the units have been setup properly the selected units will appear next to the Sy Vol and Sy ID numerical displays Refer to manufacturer specifications or manually measure using a caliper to determine the internal diameter of the syringe e Repeat the above step for syringe B 1f it is being used in this flow mode e Press BACK button to return to the setup screen 5 Dispense Withdraw Volume and Flowrate Setup e In the Constant Flow Setup screen Figure 8 press the square next to volume numerical display under syringe A e Use the pop up keypad to enter the desired volume that will be dispensed or withdrawn from the syringe e Press the square next to the flowrate numerical display under syringe A and use the pop up keypad to enter the desired flowrate for syringe A e Repeat the above step for syringe B if it is being used in Constant flow mode 6 Setup of Syringe Travel Direction e On the Constant Flow Setup screen Figure 8 under syringe A press DISP to set syringe A to dispense liquid or press WITHD to set syringe A to withdraw liquid into the syringe The set syringe direction will be displayed in the direction status box next to the status bar either D or W e Repeat the above ste
38. gure 26 to be directed to the stepped flow setup screen Figure 27 2 Select syringes by pressing Syringe A or Syringe B buttons The buttons will turn red and flash when selected Figure 27 3 Setting Up Units e Press UNITS button on the Stepped flow setup screen Figure 27 The Stepped Flow Units screen will appear Figure 28 e Select units for syringe volume internal diameter dispense withdraw volume and flowrate by pressing the labeled buttons The selected buttons will turn blue when properly activated Note Every time the pump is turned on the units need to be reset by turning ON and OFF e Units for both syringes or any one of the syringes may be selected e Once all units have been set return to the Stepped Flow Setup screen by pressing BACK button 4 Syringe Specifications Setup e Press SYR SPECS button on the Stepped Flow Setup screen Figure 27 e When the Syringe Specifications screen Figure 29 appears press the square next to HiQ Flowmate Constant Oscillatory EE Flow Flow Fu Continuous Dose E ers Flow 3 Calculator As Pulsatile a Biinsed pped Flow na 11 19 2010 Figure 26 HiQ Flowmate main screen showing stepped flow mode access button Steps 18283 SIS UNDE A0 ii Action Stepped Flow Units Syringe A S vyringe B PAUTNE Micro L inl LI Micra Lf mL LJ D oy ic pu Fea rw volume farses mo aa 3 Misre Ld Lh Pa Flowy rate mL h
39. he Stop button to been properly reset reset any active mode and then press Start The Syringe A or the Find the flow mode where Syringe B button for the the Syringe A or the active syringe shaft 1s Syringe B buttons have already activated in another been activated then press flow mode main screen to deactivate FLEXCELL9 INTERNATIONAL CORPORATION PAGE 47 FA FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE PROBLEM CAUSE TROUBLESHOOTING CORRECTION HiQ FLOWMATE FLOW MODES DO NOT START PROPERLY Cont The HiQ Flowmate syringe shaft does not move when the Start button is activated The HiQ Flowmate guide block moves extremely fast for the specific setup The HiQ Flowmate guide block moves slower than the desire flowrate for the specific setup HiQ Flowmate guide block stops during a continuous flow regimen HiQ Flowmate guide block stops during an oscillatory flow regimen before the duration time expires Some numerical inputs are missing in the flow mode setup regimen safety sensors were activated during a regimen The Control by Volume or Control by Duration button for the Pulsatile or Stepped flow mode is not activated The HiQ Flowmate is in pulsatile flow mode and the pulse duration was greater than the cycle time The units selected or the user unit conversion may be incorrect The previously active units ha
40. he syringe specifications screen where common syringe IDs and volumes are shown All screens excluding main screen Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Shear Stress Calculator Dose Calculator Constant Flow Pulsatile Flow Parameters Stepped Flow Oscillatory Flow Parameters Constant Flow Pulsatile Flow Parameters Stepped Flow Oscillatory Flow Parameters Continuous Flow Pulsatile Flow Oscillatory Flow All Syringe Specifications screens PAGE 19 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES o gt SS HARDWARE OVERVIEW Touch Screen Switch Category General Switches Constant Flow Units Syringe Specifications Continuous Flow Units Pulsatile Flow Parameters Pulsatile Flow Used to return to the Units Stepped previous screen Flow Units Stepped Flow Setup Stepped Flow Control Oscillatory Flow Parameters Oscillatory Flow Units Used to go to the Stepped Flow Control screen where the type of control volume Stepped Flow or duration for the stepped flow can be established Used to go to the Stepped Flow Setup screen where the flowrates for the stepped PEPPER EION flow can be entered e Function Keys Six function keys are located on the touch screen frame The first three function keys can be used for different tasks as follows F1 System date and time Used to set the date and time of the s
41. help screens provide brief guidelines on how to setup a flow regimen in the stepped flow mode FLEXCELL9 INTERNATIONAL CORPORATION PAGE 36 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLU FLOW MODULE OPERATION OSCILLATORY FLOW MODE Oscillatory Flow Oscillatory flow mode can be used to generate oscillatory fluid flow cyclic forward and reverse flow through a target area The two syringes can be controlled independently The existing 3 way valve assembly provided with the system can be used to generate the oscillations with one syringe A secondary valve assembly would be required to run the second syringe under oscillatory flow The tubing connection between the valve and the target area flow chamber is different from that of the continuous flow mode The user will need to set the fluid flowrate forward duration reverse duration and duration of the flow regimen using the touch screen Tubing Connection to Valve Manifold A single valve unit consisting of two 3 way valves is used for each syringe to create oscillatory flow Figure 34 These valves switch on and off to change the direction of fluid flow create oscillatory flow through the target area The fluid flows out into a reservoir not provided with HiQ Flowmate after flowing through the target area It is important to connect tubing with the valve manifold accurately to maintain proper fluid flow e Connect tubing from syringe A to port C of the valve manifold
42. ial Delrin Operating Temperature Range 10 C to 40 C 50 F to 104 F Display 5 7 inch QVGA STN color LCD Touch screen Power 100 to 240 VAC 50 60 Hz 150 W 17 9 H x24 3 W x 40 1 L cm 7 0 H x9 6 W x 15 8 L in Dimensions Weight 17 5 Lbs 7 95 Kg Environment Operating Temperature 4 C to 40 C 40 F to 104 F Storage Temperature 10 C to 60 C 14 F To 140 F Humidity 20 85 RH Non condensing Regulatory Certifications CE RoHS FLEXCELL INTERNATIONAL CORPORATION PAGE 53 m FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES gt 0 SPECIFICATIONS Fluid Flow Specifications Option A Flow Modes Constant Continuous Stepped and Oscillatory Flow Flow Mode Pulsatile Syringe ID mm Min Flowrate Max Flowrate Max Flowrate Volume 0 5uL 0 103 4 pL min 0 6 uL min 0 41 uL min 1 0 uL 8 pL min 1 27 uL min 0 87 uL min 17 pL min 2 49 uL min 1 70 uL min 5 uL 4 pL min 6 16 uL min 4 19 uL min 10 uL 80 pL min 11 97 uL min 8 14 uL min 25 uL 201 pL min 30 13 uL min 20 49 uL min 50 uL 400 pL min 59 99 uL min 40 79 uL min 100 uL 804 pL min 0 12 mL min 81 97 uL min 250 uL 1 99 nL min 0 29 mL min 0 20 mL min ImL 8 01 nL min 1 20 mL min 0 82 mL min 28 27 nL min 4 24 mL min 2 88 mL min 10 30 39 99 nL min 5 99 mL min 4 08 mL min 14 57 80 03 nL min 12 00 mL min 8 16 mL min 19 13 0 14 uL min 20 69 mL min 14 07 mL min 26 70 0 27 uL min 40 31 mL min 27 41 mL min 26 70 0 27 uL min 40 31 mL min 27 41 mL min 140 m
43. k accurately Once all units and syringe specifications have been set return to the Continuous Flow Setup screen by pressing BACK button e Check and confirm that the selected units appear next to the volume and flowrate Fl Rate inputs in the Continuous Flow Setup screen Figure 15 4 Dispense Withdraw Volume and Flowrate Setup e n the Continuous Flow Setup screen Figure 15 press the square next to the volume numerical display e Use the pop up keypad to enter the desired volume that the syringes should dispense and withdraw during one cycle of syringe travel s Continuous Flow Units In the continuous flow mode in a single cycle of sy vol EBEN L E Sy 0 DEINEN GNE syringe travel one syringe may travel from rear to front end dispense while the other syringe cue m may travel from front to rear end withdraw or i SE WEE Jung A vice versa The volume entered on the ae ae Continuous Flow Setup screen is the volume of Syringe Specifications liquid dispensed or withdrawn from each Se 10 0 se syringe during its travel from one end to the s m ECR oom other This volume can also be termed as the exchanged volume as liquid does not flow out of the syringes in the continuous flow mode Figure 16 Continuous Flow Units and Syringe Specificati l e To enter flowrate press the square next to the eee gen eee ee e flowrate numerical display and use the pop up keypad to enter the desired flowrate for both syri
44. lid into the front thrust plate in the guide block Slide the syringe barrel plate into the rear thrust plate Fig 3 part L in the front bracket Tighten the thumb screws in the guide block and front bracket to secure the syringe barrel and plunger Tighten the thrust bar thumb screw to clamp the syringes vertically Fig 3 part N Do not over tighten thrust bar thumb screw as it can increase the friction between syringe plunger and barrel and restrict smooth travel of syringe plunger Safety Sensors Safety Sensor Location and Function The HiQ Flowmate pump is equipped with four safety sensors two for each drive shaft These photomicrosensors switch ON when a reflective surface is placed in front of the sensing face The safety sensors are installed flushed with the top surface of base of the drive system at the front and rear end of each drive shaft Fig 3 part H amp R The sensors prevent the guide blocks from hitting the back and front end blocks of the drive system and causing damage to the lead screw syringes and guide blocks The sensor will be activated if the guide block travels over the sensing surface covering at least half of the sensing surface The sensors will not function properly if the sensing surface is damaged scratched or covered from dust or other matter Therefore it is important to periodically clean the surfaces to maintain proper functionality of the sensors Refer to Maintenance section pg 44 for instru
45. low to sterilize 1 Turn ON the HiQ Flowmate by switching the main power switch to ON in the back panel 2 On the menu screen select CONTINUOUS FLOW MODE 3 If you plan to use the HiQ Flowmate to flow 70 Ethanol through the valve and manifold first mount syringes 5 10 mL filled with 70 Ethanol on the pump and connect tubing from syringe 1 and 2 to port A and B of the manifold respectively 4 Connect tubing to port C and D with the open end of tubing connected to a container with 5 mL 70 ethanol Make sure that the tube is immersed in the ethanol and the container is closed to maintain sterility 5 Go to UNITS amp SYRINGE SPECS screen from the CONTINUOUS FLOW screen set units for the continuous flow to have Sy Vol mL Volume mL Flowrate mL min and Sy ID mm 6 Input syringe specifications 5 mL 10 30 mm or 10 mL 14 57 mm You may use other values in accordance with your syringe specifications FLEXCELL9 INTERNATIONAL CORPORATION PAGE 45 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES 0 MAINTENANCE 7 On the CONTINUOUS FLOW screen set values to Volume 2mL Flowrate ImL min and Duration 8 mins Select syringe A to dispense and syringe B to withdraw at the start These settings will flow 2mL of 70 ethanol per cycle of syringe a cycle is a single unidirectional travel of a syringe through the valves for a duration of 8 mins You may increase the volume based on the volume specifications of the syringe The
46. lures which result from accident or disaster such as fire explosion flood wind lightning or earthquake or misuse abuse neglect mishandling misapplication alteration faulty installation modification or service by anyone other than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty This warranty is extended only to the original purchaser customer unless a transfer of ownership is approved by Flexcell in writing LIMITED LIABILITY Flexcell or its distributor s only liability shall be to remedy any defect to comply with its warranty and return the repaired equipment to function as designed Under no circumstances shall Flexcell or its distributors be liable for any special incidental or consequential damages based upon breach of warranty or contract or negligence Such damages include but are not limited to loss of profits revenue loss of data down time customer s material or time DISCLAIMER OF WARRANTIES The Limited Warranty expressed in the foregoing language is the only warranty applicable to this product Any other warranty expressed or implied warranty or of merchantability or fitness for a particular purpose are hereby disclaimed No oral or written information or advice provided by Flexcell through its agents or employees in the use and functioning of the equipment shall in any way create a warranty or in any way increase the scope of this limited warra
47. nce to sterilize the valve unit between uses to prevent any contamination Refer Maintenance section pg 44 for instructions on how to sterilize the valve unit Flow Modules that Utilize the Valve Manifold The valve unit is utilized in continuous and oscillatory flow modules In the continuous flow mode the valve unit is connected with both syringes to create unidirectional continuous fluid flow through a target area The valves are synchronized with the syringes to maintain the direction of fluid flow through the target area when the syringes change travel direction For the oscillatory flow mode a separate valve unit 1s needed for each syringe to create oscillating flow through a target area The built in valve unit supplied with the HiQ Flowmate is configured to work with syringe A FLEXCELL9 INTERNATIONAL CORPORATION PAGE 11 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES o gt OOOO HARDWARE OVERVIEW Introduction to Touch Screen e Start up Screen The touch screen displays the start up screen Figure 5 whenever the system is first switched ON Press START to go to the main screen Figure 5 HiQ Flowmate start up screen e Main Screen The main menu screen Figure 6 displays the activation buttons for all fluid flow modes as well as buttons to go to Dose calculator Shear Stress calculator and Flow Modes description screens Press the button with the symbol to select a specific flow mode or function
48. next to Animal Weight e Use the pop up key pad to enter the weight e Repeat the above steps to enter values for drug concentration duration and flowrate e Press CAL DOSE button Figure 43 yellow oval to calculate dose e The calculated value will appear in the Figure 44 Dose Calculator Units screen numerical display below the button Duration Calculation FLEXCELL9 INTERNATIONAL CORPORATION PAGE 41 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE CALCULATION TOOLS e On the Dose Calculator Setup screen press the square next to Animal Weight e Use the pop up key pad to enter the weight e Repeat the above steps to enter values for drug concentration dose and flowrate e Press CAL DURATION button Figure 43 green oval to calculate duration e The calculated value will appear in the numerical display below the button Flowrate Calculation e On the Dose Calculator Setup screen press the square next to Animal Weight e Use the pop up key pad to enter the weight e Repeat the above steps to enter values for drug concentration dose and duration e Press CAL FLWRATE button Figure 43 white oval to calculate flowrate e The calculated value will appear in the numerical display below the button 4 Press MENU button to return to the main menu screen Shear Stress Calculator This tool can be used to calculate fluid shear stress in dynes cm when viscosity of the fluid flowrate
49. nge travel can be stopped by pressing the button with the red square 1 e the STOP button Figure 12 e Press pause button button with two yellow lines to pause the syringe travel Figure 12 If syringe travel 1s paused press START button again to restart syringe travel The syringe will restart from the paused state and continue to dispense withdraw remaining amount of liquid e Completion of Flow Regimen The flow regimen status display Figure 12 white oval will show FIN when the flow regimen is completed It will show a green background while the flow regimen is being run 8 Termination of Flow Regimen e Once the syringes have stopped after completing a regimen press STOP button to reset the system e Turn OFF the respective syringe by pressing syringe A or syringe B button The background color of the button should turn black when properly turned OFF FLEXCELL9 INTERNATIONAL CORPORATION PAGE 23 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE S FLU FLOW MODULE OPERATION CONTINUOUS FLOW MODE e Use the MENU button to return to the main menu if you need to select another flow mode e Before turning off the system use the MENU button to go back to the main menu If the syringe A and or syringe B is not turned OFF in this mode prior to exiting this mode you will not be able to use the same syringe in in any other flow mode in e Help Screen Press HELP button to access the help information on Constant flo
50. nges 5 Duration Setup e You may use the duration input on the Continuous Flow screen to setup the duration for the continuous flow regimen Figure 18 e Press the square next to each numerical input of days DD hours HH minutes MM and seconds SS and use the pop up keypad to enter the values for each of these time parameters Figure 18 red oval Figure 17 Continuous Flow Syringe Specifications screen with pop up keypad FLEXCELL INTERNATIONAL CORPORATION PAGE 26 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE S FLU FLOW MODULE OPERATION CONTINUOUS FLOW MODE 6 Setup of Syringe Travel Direction e The syringe travel direction may be setup based on the direction of flow through the soo L Valve Control target area Fl Rate 2 00 f a GE e Prior to selecting the travel direction for 2A syringes confirm that the tubing connection I4 Eu CI IB a c ELLE NE configuration in the valve manifold is correct Syringe A Synnge B with respect to the fluid flow inlet and outlet thd of the target area To select initial syringe travel direction press DISP or WITHD under syringe A and syringe B on the Continuous Flow Setup screen Figure 15 Figure 18 Setting up of duration for The right red directional indicators under the continuous flow regimen dispense and withdrawal buttons Figure 15 white oval will turn yellow and remain yellow during the flow regimen
51. ns press UNITS amp SY SPECS button on the screen The Continuous Flow Units screen will appear Figure 16 e Select units for syringe volume internal diameter dispense withdraw volume and flowrate by pressing the labeled buttons The selected buttons will turn blue when properly 5 00 f Valve Contro activated Note Every time the pump is Fi Rate ERA mL min turned on the units need to be reset by me fi Figure 14 HiO F Flowmate main screen showing continuous flow mode access button turning ON and OFF Duration Mil miei cB LADO Syringe A Syringe B 3 Syringe Specifications Setup oso vo RTT NM o e On the Continuous Flow Units screen under syringe specifications Figure 16 press the TTITES ur square next to the numerical input besides the AA E Sy Vol and Sy ID ah e Enter the volumes and internal diameters of Figure 15 Continuous Flow Setup screen FLEXCELL INTERNATIONAL CORPORATION PAGE 25 g FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE S FLU FLOW MODULE OPERATION CONTINUOUS FLOW MODE the syringes using the pop up key pad Figure 17 If the units have been setup properly the selected units will appear next to the Sy Vol and Sy ID numerical displays Refer to manufacturer specifications or manually measure using a caliper to determine the internal diameter of the syringe You should use similar syringe sizes on the two drive shafts for the continuous flow mode to wor
52. nty DISCLAIMER LANGUAGE This warranty document accompanying instruction manual and supplemental applicable laws appear in the English language In the event of any inconsistency in the meaning of the words and terminology and any foreign language translation the English language shall prevail FLEXCELL9 INTERNATIONAL CORPORATION PAGE 2 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE WARRANTY 2 GOVERNING LAW The performance of the duties and liabilities of the parties under the terms and conditions of this Limited Warranty shall be governed in all respects by the laws of the State of North Carolina the United States of America APPLICATION OF STATE LAWS Some states do not allow the exclusion or limitation of consequential damages nor do some states allow limitations on how long an implied warranty lasts so the above limitations may not apply to you This warranty gives you specific legal rights and you may also have other rights that vary from state to state 3 INTERNATIONAL CUSTOMERS The full text of the foregoing limited warranty and all disclaimers is applicable to international customers purchasers except when the purchase was made from an international distributor or reseller the warranty will be covered through your distributor or reseller If technical advisory support service is not available through your distributor or reseller for service contact warranty headquarters by phone or fax Within the United States
53. only toll free 1 800 728 3714 Fax 1 919 732 5196 International Customers telephone 01 919 732 1591 Fax 01 919 732 5196 Email info flexcellint com FLEXCELL INTERNATIONAL CORPORATION 2730 Tucker Street Suite 200 Burlington NC 27215 1 800 726 3714 within the U S only 1 919 732 1591 fax 1 919 732 5196 http www flexcellint com CAUTION HiQ Flowmate is for research use only Not for clinical use on patients FLEXCELL INTERNATIONAL CORPORATION PAGE 3 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES gt 0 0 QR STATEMENT FEDERAL COMMUNICATIONS COMMISSION RADIO FREQUENCY INTERFERENCE STATEMENT This computing equipment has been type tested and found to comply with the limits for a Class B digital device in accordance with the specifications for Subpart J of Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful radio and TV interference in a residential installation There is no guarantee that interference will not occur in a particular installation The following instructions and precautions must be observed by the user when installing and operating this device l The equipment must be operated in strict accordance with the manufacturer s instructions 2 Theunit s must be plugged into a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 Theunit must be operated with the factory installed cover in place 4 No
54. or activated during the volume to ensure adequate stepped flow regimen before regimen time or movement is the duration or the full allowed for the guide block volume is completed to complete the regimen HiQ FLOWMATE VALVE UNIT TUBING SYSTEM OPERATES IMPROPERLY The HiQ Flowmate fluid gt The tubing system was Check the Fluid Flow flow in the valve unit tubing connected incorrectly for Module Operation section system is blocked the continuous or for proper tubing valve unit oscillatory flow mode connections gt A valve needle used to Examine the valve needles connect the tubing to the for visual verification valve unit 1s deformed and Contact Flexcell if any limiting or blocking flow deformations are found gt Air pockets are presentin gt Fill system completely with the system the desired experimental fluid gt A section of the tubing is Examine tubing and replace creased or blocked tubing where necessary gt A section ofthe valve unit gt Contact Flexcell is possibly blocked Air pockets appear in the gt The tubing is not properly Drain the system of air tubing during an oscillatory submerged in the reservoir pockets and then securely flow regimen when setup to fluid container submerge the tubing in the withdraw reservoir fluid Air pockets appear in the gt A perforation or laceration gt Examine tubing and replace tubing line occurred in the tubing tubing where necessary The
55. p the cycle time in minutes and seconds Press the square next to the numerical display to open up a pop up keypad to enter values for minutes and seconds M S Used to enter the number of cycles in the stepped flow mode Press the square next to the numerical display to open up a pop up keypad to enter a value for the cycle number Used to enter the Forward Duration of flow in minutes M and seconds S Used to enter the Reverse Duration of flow in minutes M and seconds S Pulsatile Flow Parameters Pulsatile Flow Parameters Stepped Flow Oscillatory Flow Oscillatory Flow Status bar on left will display the syringe control status Start Pause or Stop Constant Flow Pulsatile Flow FLEXCELL INTERNATIONAL CORPORATION The square on right displays Stepped Flow the direction of syringe Oscillatory Flow travel D for dispensing and W for withdrawal PAGE 17 A FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES lt ia_OSS COCOCOQAARDWARE OVERVIEW Touch Screen Switch Category Status Displays Level meter displays the volume of liquid currently dispensed from or withdrawn in to the syringe with units Displays the status of four safety sensors mounted in the base two at the front and two at the back The Constant Flow indicators will flash when Continuous Flow sensors are activated Pulsatile Flow zd rr Terminate the program by Stepped Flow pres
56. p to set the direction of travel for syringe B if it is being used in constant flow mode Please make sure that syringes e have enough distance syringe sizes are accurate from the ends to travel directions in their respective Constant Flow Units S yringe A Syringe B Micro L ne BL ii Eui mw IMiere C mL pL ma B mL ErETEE intimin M pLimin Sy Yol Micro L um DL Sy ID um em iin Mice fme ImL min d mL J ETE REDE nLimin If pLimin Volume ow rete Figure 9 Constant Flow Units screen Syringe Specifications Syringe A ovringe B EXCE soc BENE soo fue XT Ios m Figure 10 Constant Flow Syringe Specifications screen Figure 11 Constant Flow Syringe Specifications screen with pop up keypad FLEXCELL INTERNATIONAL CORPORATION PAGE 22 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLUID FLOW MODULE OPERATION CONSTANT FLOW MODE Syringe Control Status Bar The syringe control status bar 1s below the control buttons and displays the current syringe control status Figure 12 When the syringes are not in use it will display Status It will display Started when a flow regimen is started Paused when a flow regimen is paused and Stopped when a flow regimen is stopped Syringe Level Display The syringe level a T display will show the volume of liquid in each Syringe A Syringe B syringe in real time Figure 12 If the syringe
57. pply a thin layer of lubrication oil to the guide bars using a lint free cloth or a brush Avoid using excessive oil on the guide bars When repositioning guide blocks on the lead screw fully press the lock nuts to completely disengage the lock nuts from the lead screw If the lock nut is partially pressed it will rub against the lead screw and cause it to wear Do not use the valves or the valve manifold with liquids or gases at temperatures exceeding 409C 1049F or below 109C 509F to as they can damage the seals and diaphragms in the valves Periodic cleaning and sterilization should be performed to prevent fouling of internal channels of the valve and manifold The HiQ Flowmate is installed with a cooling fan to provide adequate cooling to the internal components In order to maintain efficient cooling always make sure that the fan guard is free from dust and other material that may block the air flow Position the HiQ Flowmate so that its back wall is at least 3 inches away from walls flat surfaces to ensure sufficient space for air flow gt Touch Screen Care The touch screen should not be exposed to temperatures exceeding 509C 122 F or temperatures below 0 C 32 F Do not expose the touch screen to high humidity that may result in condensation It should not be exposed to chemicals corrosive or flammable gases oil excessive shock or vibration or strong ultraviolet light In order to keep the touch screen clean wipe the surfa
58. r 1l mL min Il mL hr il JE M pumin i iin SEIN a Hep a Figure 28 Stepped Flow Units screen the numerical inputs of Sy Vol and Sy ID under syringe A FLEXCELL INTERNATIONAL CORPORATION PAGE 33 f FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE FLUID FLOW MODULE OPERATION STEPPED FLOW MODE e Use the pop up keypad to enter the volume Ii Syringe Specifications and internal diameter of the syringe Figure l 30 If the units have been setup properly the ATi selected units will appear next to the Sy Vol sy Vol G mi and Sy ID numerical displays Refer to OM 14 57 fee manufacturer specifications or manually measure using a caliper to determine the Syringe B internal diameter of the syringe TAEA 5 00 MED e Repeat the step above for syringe B if it is Sy ID E being used in the stepped flow mode MESES e Press BACK button to return to the Stepped Flow Setup screen figure 27 Figure 29 Syringe Specifications screen for stepped flow mode 5 Setting Up Fluid Flowrates for Step Stages m Syringe Specifications e In the Stepped Flow Setup screen press the button with the yellow step symbol Figure Numeric keypad 27 white circle The Flowrate Setup screen of the stepped flow mode will appear Figure 31 e Press the square next to the Flowrate 1 numerical input e Use the pop up keypad to enter the desired value for flowrate 1 This is the flowrate of Eri stepping stage 1 ED
59. r syringe A press DISP to set the syringe A to dispense liquid or press WITHD to set syringe A to withdraw liquid into the syringe Figure 41 The set syringe direction will be displayed in the direction status box next to the status bar either D for dispense or W for withdraw e Repeat these steps to set the direction of travel for syringe B if it is going to be used in oscillatory flow mode Oscillatory Flow evrindge A evringe B Please make sure that the OMEEHLLLIRnRnRRRAL LEHRRIGEEELLLLLLLLLOEGCOLOOzc DB Bbi h E lBIXLGL dGdG4e l M Rate 0 50 mifmin 0 25 mbmin syringes have enough distance ETUOPUNEUNSETUERUUUUSUUNUURU UU e F 4 a 55 O 10 0 MM 5 from the ends syringe sizes are Fw dur O 4 0 mmiss O 210 0 MM SS accurate to travel in their Rvdur 0 5 0 MM ss 0 3 0 mm ss respective directions Time lett 2 oo o0 au 00 00 00 00 DD HH MM 8 Flow Regimen Control e Prior to starting the flow regimen make sure that the sensors are all inactive no red sensor indicators in the safety sensor section Figure 41 yellow oval e Press RUN button green triangle to start syringe motion The syringe travel can be stopped by pressing the button with the red square 1 e the STOP button Figure 41 e Press PAUSE button button with two yellow lines to pause the syringe travel Figure 41 If syringe travel is paused press START button again to restart syringe travel Th
60. s will be Parameters displayed in front of the numerical input Another numerical input for the flowrate This input Flowrate 1 12 50 mL min is numbered as there are Stepped Flow several flowrate inputs in stepped flow mode Duration input is used to enter values for the duration parameters Press Continuous Flow rcu soo is MUI MESSM squares next to DD days Oscillatory Flow HH hrs MM minutes Parameters SS sec and use the pop up keypad to enter values Another duration input In addition to duration setup the blue button below the Duration DD HH MM SS numerical inputs is used to select the pulsatile flow Pulsatile Flow an o L all maf control by duration Press Parameters the blue bar and the square next to it will turn green if the duration control 1s activated Duration numerical input of stepped flow mode Press the squares next to 1 ill 20 30 the numerical input to open A Control a key pad and enter the values for duration in hrs mins and seconds FLEXCELL INTERNATIONAL CORPORATION PAGE 16 g FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW Touch Screen Switch Category Numerical Inputs Ste Ol 0 0 ES Cyc time 30 5 0 M S Used to setup the pulse duration in minutes and seconds Press the square next to the numerical display to open up a pop up keypad to enter values for minutes and seconds M S Used to setu
61. satile flow control by volume Press the red bar and the square next to it will become green if the volume control is activated syringe volume numerical input is used to input volume of the syringe used Press the square next to display and a key pad will pop up Type the desired value and press enter Selected units will be displayed in front of the numerical input syringe ID numerical input is used to input internal diameter of the syringe used Press the square next to display and a key pad will pop up Type the desired value and press enter Selected units will be displayed in front of the numerical input Constant Flow Continuous Flow Stepped Flow Control Pulsatile Flow Parameters Syringe Specifications screens of Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow Syringe Specifications screens of Constant Flow Continuous Flow Pulsatile Flow Stepped Flow Oscillatory Flow FLEXCELL INTERNATIONAL CORPORATION PAGE 15 A FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES o gt HARDWARE OVERVIEW Touch Screen Switch Category Numerical Inputs Flowrate numerical input is used to input flowrate Constant Flow Fl Rate 0 00 Press the square next to Continuous Flow display and a key pad will Pulsatile Flow pop up Type the desired Parameters 3 value and press enter Oscillatory Flow FI Rate 1 50 mL min Selected unit
62. sing stop button and Oscillatory Flow move the guide block away from the sensor before restarting the program Displays the status of the two valves Flow out indicates liquid flowing out Continuous Flow Constant Flow Sensors Valve Control of the valve Flow in indicates liquid flowing into the valve Displays the amount of time left in the current Pulsatile Flow program in days Hrs Oscillatory Flow Mins Secs Displays the forward duration of liquid flow in U MM SS d Fw dur 0 20 0 mcus MN dad seconds Oscillatory Flow SS Displays the reverse duration of liquid flow in t MM ss Rv dur 0 10 0 mimes NND and seconds Oscillatory Flow SS Time left 00 00 01 59 FLEXCELL INTERNATIONAL CORPORATION PAGE 18 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE HARDWARE OVERVIEW Touch Screen Switch Category General Switches FLEXCELL INTERNATIONAL CORPORATION Used to access more information on the current screen or how to set parameters on the current screen Used to return to the main menu screen of the HiQ Flowmate Used to go to the syringe specifications screen of the current flow mode Used to go to the unit setup screen of the current flow mode Used to go to the unit setup and syringe specification screen of the continuous flow mode Used to go to the parameter setup screen of the current flow mode Used to go to t
63. stant Ose ETC mel atory Flow BEEN AE nw Calculator Calculator Setup 1 Press the DOSE CALCULATOR symbol button on the HiQ Flowmate main screen Figure 42 to be directed to the Dose Calculator screen Figure 43 Continuous E Dose Dose TR orte Flory Pulsatile BECA n Steeped i pped a n 2 Setting Up Units 11 19 2010 e Press UNITS button on the Dose Calculator Figure 42 HiQ Flowmate main screen screen Figure 43 showing Dose calculator access button e On the units screen select units for weight Ag Dose Duration Flowrate Calculator dose concentration duration and flowrate by nekeng 2 500 NES pressing the labeled buttons Figure 44 The Drug Concent 0 500 mom selected buttons will turn blue when properly Dose L 0 500 MEET activated Note Every time the pump is turned Serie 0 500 MET on the units need to be reset by turning ON and enn rr Ped OFF Nt TI Cal Duration JN e Press BACK button to return to the Dose 0 000 Calculator Setup screen EC Min mL min e Check and confirm that the selected units linis J Menu f Hep appear next to all numerical inputs on the Dose Calculator Setup screen The selected units Figure 43 Dose Calculator Setup screen should also appear below the results display Le Dose Duration Flowrate Calculator Units 3 Calculator Operation gt Dose Calculation e On the Dose Calculator Setup screen press the square
64. syringe tubing Properly seal the connection is not secured syringe tubing attachment and sealed properly connection gt The target tubing gt Properly seal the connection was not secured tubing target attachment and sealed properly connection FLEXCELL INTERNATIONAL CORPORATION PAGE 49 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE8 sss APPENDIX Appendix Pulsatile Flow PULSE DURATION CONSTANT FLOWRATE FLOWRATE CYCLE TIME NOTE PULSE RATE 1 CYCLE TIME Figure 47 Pulsatile flow mode signal representation gt Pulsatile flow mode Figure 47 is used to generate pulse flow in either withdraw or dispense direction The pulsatile flow can be controlled in time scale or volume scale such that the syringes can be programmed to maintain a pulsatile flow for a specified time or to dispense withdraw a specified volume Stepped Flow FLOWRATE 3 FLOWRATE 4 FLOWRATE R OR OR VOLUME VOLUME VOLUME VOLUME 1 2 3 4 Figure 48 Stepped flow mode signal representation gt The stepped flow mode Figure 48 may have up to four different flowrates in one cycle Depending on the control settings each step may have a unique duration or may have a unique volume FLEXCELL INTERNATIONAL CORPORATION PAGE 50 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES sss APPENDIX gt Oscillatory Flow RESERVOIR Figure 49 Oscillatory flow mode liquid oscillation diagr
65. that syringes have enough distance from the ends syringe sizes are accurate to travel in their respective directions 10 Flow Regimen Control e Prior to starting the flow regimen make sure that the sensors are all inactive no red sensor indicators in the Safety Sensor section Figure 33 white oval e Press RUN button green triangle to start syringe motion The syringe travel can be stopped by pressing the button with the red square 1 e the STOP button Figure 30 e Press PAUSE button button with two yellow lines to pause the syringe travel Figure 33 If syringe travel is paused press START button again to restart syringe travel The syringe will restart from the paused state and continue through its step stages 11 Termination of the Flow Regimen e Once the syringes have stopped after completing a regimen press STOP button to reset the system e Turn OFF the respective syringe by pressing syringe A or syringe B button Figure 33 The background color of the button should turn black when properly turned OFF e Use the MENU button to return to the main menu if you need to select another flow mode e Before turning off the system use the MENU button to go back to the main menu If the syringe A and or syringe B is not turned OFF in this mode prior to exiting this mode you will not be able to use the same syringe in any other flow mode Help Screen Press HELP button to access the help information on stepped flow mode The
66. ther flow modes Help Screen Press HELP button to access the help information on Continuous flow mode The help screens provide brief guidelines on how to setup a flow regimen in the continuous flow mode HiQ Flowmate Constant Oscillatory EE Flow Flow Fa Continuous my Dose P E Flow Calculator Az Pulsatile Flow Pulsatile flow mode is used to generate pulse flow in either withdraw or dispense direction The travel of the syringes is controlled independently allowing the user to conduct multiple experiments simultaneously The user needs to set the fluid flowrate cycle time and pulse on duration using the touch screen The pulsatile flow can be controlled in time scale or volume scale such that the syringes can be ESSEEN i programmed to maintain a pulsatile flow fora Figure 19 HiQ Flowmate main screen specified time or to dispense withdraw a showing Pulsatile flow mode access button specified volume Pulsatile Flow N ET pped How Pw Pulsatile Flow aum Syringe A l Era Flow Regimen Setup CITAN 1 Press the PULSATILE FLOW symbol button i on the HiQ Flowmate main screen Figure 19 to be directed to the Pulsatile Flow Setup SS i screen Figure 20 Disp B cno CE Disp BB witha Sensors 2 Select a syringe by pressing the Syringe A or a 1 O rest EET zu I Syringe B button The buttons will turn red EDU u Mena Figure 20 Pulsatile Flow Setup screen FLEXCELL I
67. tress Calculator screen press the square next to Dyn Viscosity e Use pop up key pad to enter the viscosity e Repeat the above step to enter values for flowrate channel width and channel height e Press CALCULATE button to calculate the shear stress in dynes cm e The calculated value will appear in the numerical display above the button e Press MENU button to return to the main menu screen FLEXCELL9 INTERNATIONAL CORPORATION PAGE 43 E FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE MAINTENANCE Maintenance gt General Instrument Care In order to maintain components of the HiQ Flowmate in proper working condition we suggest that you do the following 1 2 3 4 5 6 7 8 9 Maintain a dust and lint free environment where the HiQ Flowmate is placed Wipe off dust or lint from all surfaces using a lint free cloth periodically Do not spill liquid oil and chemicals or keep any dripping liquid containers or vessels on top of HiQ Flowmate casing as the liquid can seep into the box and harm circuitry Avoid using HiQ Flowmate near large liquid containers or vessels To ensure long life of the lead screw periodically apply a small amount of lubrication oil GL 4 or GL 5 oil to the lead screw using a lint free cloth or a brush Avoid using excessive oil or grease on the lead screws Do not expose the lead screw to bleach or chlorine solutions as these solutions can corrode the lead screw A
68. values e Press the squares next to the pulse duration Pls dur minutes M and seconds S numerical displays under syringe A and use the pop up keypad to enter the desired values e Repeat the above steps 1f syringe B 1s being used in Pulsatile flow mode Syringe Specifications Figure 24 Pulsatile Flow Syringe Specifications screen with pop up keypad Cycle time and Pulse Duration Minimum pulse duration 0 3 sec Minimum cycle time 0 4 sec Cycle time must always be greater than pulse duration 6 Pulsatile Flow Control by Duration or Dispense Withdraw Volume gt Control by Volume e On the Pulsatile Flow Parameters screen press the red bar below the volume numerical Vol input Figure 21 white oval to activate the flow regimen control by volume The indicator next to the red bar should turn green if properly activated e Press the square next to the volume numerical input e Use the pop up keypad to enter the dispense or withdraw volume for syringe A e Repeat the above steps if syringe B is being used in Pulsatile flow mode gt Control by Duration e On the Pulsatile Flow Parameters screen press the blue bar below the Duration numerical input Figure 21 white oval to activate the pulsatile flow regimen control by time The indicator next to the blue bar should turn green if properly activated e Press square next to numerical input for SS seconds under Duration e Use the pop up keypad to enter se
69. ve A will be in Flow out and valve B will be in Flow in mode The valve status will change alternatively when syringes change travel direction after each cycle during continuous flow 7 Flow Regimen Control e Prior to starting the flow regimen make sure that the safety sensors are all inactive There should be no red sensor indicators in the safety sensor section lower right corner of the Continuous Flow screen see Figure 18 FLEXCELL9 INTERNATIONAL CORPORATION PAGE 27 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE S FLU FLOW MODULE OPERATION CONTINUOUS FLOW MODE e Press RUN button green triangle to start syringe motion The syringe travel can be stopped by pressing the button with the red square 1 e the STOP button Figure 18 Press PAUSE button button with two yellow lines to pause the syringe travel Figure 18 If syringe travel is paused press START button again to restart syringe travel The syringe will restart from the paused state and continue to run the programmed flow regimen 8 Termination of Flow Regimen e Once the syringes have stopped after completing a regimen press STOP button to reset the system e Use the MENU button to return to the main menu if you need to select another flow mode e Before turning off the system use the MENU button to go back to the main menu X Since both syringes are utilized during Continuous flow mode none of Oe the syringes can be used simultaneously with any o
70. ve not been properly de selected The units selected or the user unit conversion may be incorrect The previously active units have not been properly de selected A safety sensor was activated during the regimen A safety sensor was activated during the regimen FLEXCELL9 INTERNATIONAL CORPORATION gt Check all the inputs needed to setup the regimen to ensure they all have numerical values Move the guide block into a position outside of the safety sensor proximity Press the Control by Volume or Control by Duration button for the pulsatile or stepped flow mode Adjust the cycle time to be greater than the pulse duration Minimum pulse duration 1s 0 2 seconds Check the units for the specific flow mode in operation De select all units and re select the desired units Check the units for the specific flow mode in operation De select all units and re select the desired units Check the volume dispensed or withdrawn to ensure adequate guide block movement is allowed Check the duration to ensure adequate time 1s allowed for guide block movement Valves control oscillation and the syringe moves unidirectional PAGE 48 z FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATES 0 TROUBLESHOOTING PROBLEM CAUSE CORRECTION HiQ FLOWMATE OPERATES AT AN UNDESIRED FLOWRATE Cont HiQ Flowmate guide block gt A safety sensor was gt Check the duration or stops during a pulsatile
71. volume o is in dispense mode it will initially display the aS liquid volume that is being set to dispense and sis 0 50 E 2 00 MESSIS gradually decrease to show in real time the B 1 F 3 m volume of liquid that is being dispensed from essi emu umm the syringe If the syringe is in withdrawal gt m mfe ES T gt m El mode it will initially show a zero volume and Valise SEER will gradually increase to show in real time C 0 00 MEME NNI the volume of liquid that is being withdrawn units Help ff Menu into the syringe It will also show the unit that has been selected for dispense withdrawal volume Figure 12 Syringe control status bar and syringe level display Safety Sensors The status of safety sensors are displayed at the center of the setup screen Figure 12 yellow oval The actual photomicrosensors are located at each end front and rear of the lead screw in the drive system These sensors are installed to prevent the guide blocks from hitting the back and front ends of the drive system If the sensors are activated the color of display square will change from green to red In that case move the guide block sufficiently away from the sensor and restart the flow regimen 7 Flow Regimen Control e Prior to starting a flow regimen make sure that the sensors are all inactive no red sensor indicators in the safety sensor section e Press RUN button green triangle to start syringe motion The syri
72. w mode The help screens provide brief guidelines on how to setup a flow regimen in the constant flow mode Continuous Flow Continuous flow mode can be used to generate steady continuous liquid flow in one direction either withdraw dispense direction through a target area The fluid flow can be controlled based on time Both syringes are utilized to maintain a unidirectional continuous flow for a set period of time Two 3 way valves mounted onto a manifold are used to maintain unidirectional flow continuously through the target area The valves are controlled by the flow control software Both syringes travel simultaneously in opposite direction to maintain flow At the end of each travel cycle Example If syringe A in dispense mode and syringe B in withdrawal when syringe A reaches the front end and when syringe B reaches the rear end the syringes change travel direction while the two valves switch status to maintain liquid flow in one direction The initial flow directions of the syringes need to be set depending on the desirable direction of liquid flow through the target area There onwards the set direction will be maintained by the syringe control software at the set flowrate for the set period of time The volume dispensed withdrawn during each cycle of syringe travel can be set in the Continuous Flow screen The major advantage of this flow mode is that the user can continuously maintain unidirectional fluid flow through a target area
73. yringe respectively using the pop up key pad Figure 40 If the units have been setup properly the selected units will appear next to the Sy Vol and Sy ID numerical displays Refer to manufacturer specifications or manually measure using a caliper to determine the internal diameter of the syringe e Repeat the above steps for syringe B if it is going to be used in this flow mode e Press BACK button to return to the Oscillatory Flow Parameters screen Figure 37 Oscillatory Flow Parameters screen FLEXCELL9 INTERNATIONAL CORPORATION PAGE 38 f FLEXCELL INTERNATIONAL CORPORATION HIQ FLOWMATE 5 Setting Up Oscillatory Flow Parameters e n the Oscillatory Flow Parameters screen Figure 37 press the square next to the flowrate Fl rate numerical display under syringe A e Use the pop up keypad to enter the desired flowrate e Press the squares next to forward duration Fw Dur minutes M and seconds S numerical displays under syringe A and use the pop up keypad to enter the desired values e Press the squares next to reverse duration Rv Dur minutes M and seconds S numerical displays under syringe A and use the pop up keypad to enter the desired values e Repeat the above steps if syringe B is going to be used in oscillatory flow mode 6 Oscillatory Flow Control by Duration e On the Oscillatory Flow Parameters screen Figure 37 press the square next to the numerical input for SS seconds under
74. ystem F2 Contrast and brightness Used to change the contrast and brightness of the touch screen F3 System menu Used to change the communication modes of the touch screen DO NOT CHANGE ANY SETTINGS ON THE SYSTEM MENU BEFORE CONSULTING FLEXCELL INTERNATIONAL FLEXCELL INTERNATIONAL CORPORATION PAGE 20 E FLEXCELL INTERNATIONAL CORPORATION HIQFLOWMATE FLU FLOW MODULE OPERATION CONSTANT FLOW MODE Fluid Flow Module Operation Constant Flow Mode In the constant flow mode two independently driven stepper motors are used to generate precise dispensing or withdrawal of fluid at user set flowrates with different syringe sizes The fluid flow regimens can be programmed in volume scale The user will have the option of selecting a specific unit from a broad range of available units User preferred syringe sizes can be input into the program Several popular syringe specifications are also available to the user for reference The total volume that can be dispensed or withdrawn will depend on the size of the syringe used A real time level meter on the screen displays the volumes of liquid currently in the syringe during dispense or withdrawal action Flow Regimen Setup HiQ Flowmate 1 Press the CONSTANT FLOW symbol button on the HiQ Flowmate main screen Figure 7 to be directed to the Constant Flow Setup screen Figure 8 can Teama M scillatary ae la Flow E E Flow Eu Continuous Dose onte Flow A

HiQ Flowmate® Dual Syringe Pump User Manual

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HiQ Flowmate&reg; Dual Syringe Pump User Manual

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HiQ Flowmate® Dual Syringe Pump User Manual