Midi CO2 Incubators User Manual
Contents
1. Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv Midi 40 C02 Incubator Thermo Scientific Thermo Scientific Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Table of Contents eg EE 1 1 EE TPN oe WS be vara ARI 1 1 Specifications adi ne Setters Dk nde illa 1 2 Safety Precautions 2 1 Operating Standards 3 1 Pre Installation ee gedreet Ee ee seu 4 1 Eocati n e Rade ci Pee ee a Paso EE 4 1 SETA gene bie an A eles ew Rags ihe cae eed 4 1 Inelided Pats ii is AS AA Reed 4 1 CO2 Gas EE 4 2 Wiring sedii A ea nTen ree eee chao AS 4 2 Gas Supplies oii 2 ee A ASA 4 3 Component Locations citant Eege 4 4 Initial Start Up Procedures 0 cccee eee eee cence eee 5 1 Setpoint Parameters y sailor trata 5 1 Power and Key Switch sers saa Sig ie cen eg oa eas Masse aes 5 1 Start Up Prompts is nit E a a 5 1 Key Switch and Control Operation 6 1 Restore Function E EE 6 3 Service Mode Parameters o a o alero a E Wo 6 3 Setpoint and Alarm Programming atinada da 6 3 Calibration Opuonal vic o chat ote a Bool elas R 6 4 Temperature Correlation era o bt2. For any modifiable parameter the up and down arrow buttons can be used to adjust the value The display automatically returns to normal operating mode 30 seconds after the last key entry or after scrolling through all available functions and parameters Table 6 3 Service Parameters Parameter Notes Network Adr Network address for RS485 applications Default 238 SN Serial number Check Sum Checksum to identify firmware version Baud rate For RS485 applications PWM Info CO2 sensor and excursion information Restore Function When the incubator is first powered up all factory set program parameter defaults can be restored as follows Table 6 4 Restore Defaults Function Sequence Notes Press A and V simultaneously hold for five seconds Restore program defaults Restores original factory set values of all program parameters Thermo Scientific Midi 40 CO2 Incubator 6 3 Section 6 Key Switch and Control Operation Calibration Optional CO2 Measurement and Calibration This method uses an instrument such as the Fyrite to read the actual CO2 in the chamber The display can be changed to agree with the measured reading The factory standard method using traceable gas standards assures an accuracy of 0 2 The Fyrite instrument obtains 0 5 accuracy To use the Fyrite for CO2 concentration measurement complete the following steps 1 Wait at least 15 minutes after th
3. large area heater provides enough radiant heat to the glass door to control condensation The micro processor control operates the door heater This system uses a sensor assembly consisting of a pair of matched thermistor sensors in a housing One of the sensors is sealed in a nitrogen filled housing and provides the O to 1 VDC reference signal for the gas control board The other sensor continually samples filtered chamber air The main control board compares these signals with the input from the control panel CO2 setpoint If the difference exceeds 0 5 below setpoint the CO2 solenoid remains open 100 of the time If the difference is less than 0 5 the CO2 solenoid cycles until setpoint is reached When the chamber inner door is opened the door switch shuts off all gas flow into the chamber When the door is closed gas again flows into the chamber under the control of the TC gas concentration sensor At this time the gas solenoid is open 100 of the time and remains open until the CO2 level has recovered to within 5 of the setpoint The solenoid then reverts to cycling Thermo Scientific Thermo Scientific Section8 Maintenance Warning Maintenance procedures involve working with high voltages which can cause injury or death Maintenance should only be performed by trained personnel A The incubator can be easily cleaned and disinfected in about 30 minutes Be sure to use an appropriate disinfectant solution Roccal II its Lys
4. warning and informational labels attached to the incubator caution info electrical hazard O hot surface AL alternating current earth ground protective conductor terminal Figure 1 1 Symbols Used in Labels on Incubator Before installing using or maintaining this product please be sure to read this manual and product warning labels carefully Failure to follow these instructions may cause this product to malfunction which could result in injury or damage Thermo Scientific Midi 40 C02 Incubator 2 1 Section 2 Safety Precautions 2 2 Midi 40 C02 Incubator Below are important safety precautions that apply to this product e Use this product only in the way described in the product literature and in this manual Before using it verify that this product is suitable for its intended use e Do not modify system components especially the controller Use OEM exact replacement equipment or parts Before use confirm that the product has not been altered in any way e Disconnect the unit from all power sources before cleaning troubleshooting or performing other maintenance on the product or its controls To disconnect power supply to the incubator unplug the supply cord at the back of the incubator Note that turning the key switch on the front control panel to the Off position is not sufficient to disconnect power Warning The user is responsible for carrying out appropriate decontamination procedures when h
5. 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermofisher com Internet Worldwide Web Home Page service led marietta thermofisher com Service E Mail Address Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction
6. CARR ee eee 6 5 Midi 40 CO2 Incubator V Table of Contents Section 7 Control Systems Theory cece cece eee e eee eens 7 1 Overtemperature Monitoring System 0 0 00 0000 eee 7 1 CO2 Recovery after door opening 0 0 eee eee ee 7 2 CO Control EE 7 2 Door Heat System ei ecanese bide reia regs o di heres 7 2 Section8 Maintenance 8 1 CO Filter Replacement 2 hede sadn sedms da ea 8 2 Ellas id a 8 2 Door EE 8 3 FUSES A ASA Aa ena Gene Gan ee 8 3 section 9 Parts List E 9 1 Section 10 Warranty Information 10 1 vi Midi 40 C02 Incubator Thermo Scientific Thermo Scientific Requirements Section 1 Introduction These incubators are designed to create a stable reliable environment for cell culture applications They operate at temperatures ranging from 5 C above ambient temperature to 60 C accurate to 0 2 C The gas system controls within 0 1 of measurable setpoint e Thermal conductivity sensor provides stable automatic injection of CO2 e Automatic Gas Shutoff control turns off the gas supply when the inner glass door is opened to prevent wasting the control gas e Alarms High and low level CO2 setpoint alarms adjustable High and low temperature alarms high alarm can be set by user Battery Low alarm control system has tested the alarm battery and determined that it should be replaced Caution The function of the alarm battery is to operate
7. Holding the Fyrite in the upright position place the sampling rubber connector tip over the plunger valve on the Fyrite Purge the sample line by squeezing the aspirator bulb twice then epress the plunger valve firmly with the connector tip Squeeze and release the aspirator bulb 18 times During the 18th squeeze and with bulb held deflated release the connector tip and the plunger valve 6 4 Midi 40 CO2 Incubator Thermo Scientific Calibration Optional continued Temperature Correlation Thermo Scientific Section 6 Section title Note Always hold the Fyrite by the fins only to prevent warming of the Fyrite fluid during analysis A 9 Invert the Fyrite until the fluid drains into the top reservoir then turn the Fyrite upright to drain the fluid into the bottom reservoir Repeat this step once 10 Briefly hold the Fyrite at a 45 angle to allow the fluid to drain into the bottom reservoir 11 Hold the Fyrite completely upright and immediately read the CO2 percent on the scale at the point corresponding to the top of the fluid column 12 Wait a few minutes then repeat the entire procedure steps 3 through 11 to confirm the reading 13 To offset the CO2 level enter programming mode by pressing Mode and holding for 5 seconds On release the current temperature setpoint value flashes in the display Continue to press Mode until the display changes to display CO2 offset Use up and down arrow keys to adjust th
8. Soul eo1uy3a BY 0 pauunja pue pajajdwoo s pies jueJem ay PEplAOJd uogejjezsui pue JaalJap JO SUJUOW XIS jeuonippe ue pamo e ae juaudinba no 490 s oym sJajesag poled jueJem Jes jeu ayy Buunp Jaumo juaenbasqns Aue o spua xa uo 998 01d JUeJem ay palanijep s juaudinba noA aun wes y jejewixoldde je joaya O U OB Wu jueJem ay os aun Buiddiys 104 smoye et Ayyloes no wouy peddiys s juaudinba noA app ay wo syjuoul OM SUEIS pollag AIUEUEM SUL ALNVAYVM Y31W30 TVNOILVNYSLNI OIALLN3IOS YAHSIA OWYSHL Thermo Scientific Midi 40 C02 Incubator 10 2 Declaration of Conformity Manufacturers Name Thermo Fisher Scientific Asheville LLC Manufacturers Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description Laboratory Incubators Product Designations 3404 Year of Initial Marking CE 2009 Affected Serial Numbers Release 1 Release Level REL shown on Serial Tag These products conform to the following European Union Directive s EMC 89 336 EEC LVD 73 23 EEC These products conform to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 A1 1998 EN 61010 1 EN 55011 1991 Conducted Emissions Class A Radiated Emissions Class A B EN 61326 1 1997 A1 1998 Annex A Richard L Miller CQE Regulatory Compliance Manager Thermo isher SCIENTIFIC 06 November 2009 Rev 0 Thermo Scientific Midi 40 CO2 Incubat
9. like Figure 5 1 The next section TEMP C explains all the control functions available 7 0 during normal operation On initial start up 3 however a series of prompts displays This CO2 section explains the start up prompts and the procedures to follow 5 A Turn the key switch shown in Figure 5 1 to the 1 position Press and hold the up arrow button to initiate the special start up prompts If A a temperature displays instead of a start up O prompt the up arrow button hasn t been held long enough Turn the key switch back to O V and try again key switch O Wri A prompt displays to accept or adjust the values 4 of several operating parameters In each case A press the up or down arrow buttons increase or __ decrease to change a displayed value Press Figure 5 1 Control Panel Mode when the displayed value is the one desired The modifiable value is always the one flashing If a displayed parameter is not adjusted and 60 seconds elapse the software registers the existing default value and moves to the next parameter If no changes are needed to the factory set defaults press Mode repeatedly until the end of the prompts Midi 40 CO2 Incubator 5 1 Section 5 Initial Start Up Procedures S etp oint Parameters The adjustable parameters are c ontinued Temperature default 37 C e Warm alarm default 40 C cannot deviate from the temperature setpoint by less than 0 3 C e Cold al
10. Clean the inner door gasket thoroughly Clean the inside of the glass door with the disinfectant solution then rinse twice with sterile distilled water Clean the shelves support walls and pans with disinfectant and rinse thoroughly with sterile distilled water Wipe down all disinfected surfaces with an alcohol solution Caution Alcohol is volatile and flammable Use only in a well ventilated area removed from open flames and other heat sources Allow sufficient time for fumes to dissipate before using cleaned components A To replace the CO2 Filter 1 2 Turn the main power switch to OFF Turn the gas supply or supplies to OFF Remove the tubing from both ends of the gas filter Note the flow direction on the filter The side marked IN points to the gas supply Install the new filter onto the tubing connected to the incubator and the tubing connected to the gas supply refer to Gas Supplies in Section 4 and Figure 3 1 Turn the gas supply or supplies to ON Turn the main power switch to ON Thermo Scientific Fuses Door Seal Check Thermo Scientific Section 8 Section title The fuses used in this incubator are located on the power supply board and should only be replaced by a qualified technician The F1 and F2 fuses are Type T rated at 120V 6A or 230VAC 3A The F3 and F4 fuses are Type T rated at 120V 500mA or 230VAC 300mA To check the door seal complete the following steps
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12. Midi 40 Series C02 Incubator Operating and Maintenance Manual 7003403 Rev 1 Part of Thermo Fisher Scientific SCIENTIFIC Preface Models covered by this manual Model 3403 120V Model 3404 230V MANUAL NUMBER 7003403 1 26174 2 18 10 Clarifications and corrections per N Pisani CCS 0 12 1 09 Original was 33602H01 Rev A CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific Midi 40 CO2 Incubator i Preface CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance A Caution All internal adjustments and maintenance must be performed by qualified service personnel A Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual 2009 Thermo Fisher Scientific All rights reserved Midi 40 CO2 Incubator Thermo Scientific gt SS RS a AA bt ps Preface Important operating and or maintenance instructions Read the accompanying text carefully Potential electrical hazar
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14. arm default 34 C cannot deviate from the temperature setpoint by less than 0 3 C e CO2 default 5 e High CO2 alarm default 6 cannot deviate from the CO2 setpoint by less than 1 e Low CO2 alarm default 4 cannot deviate from the CO2 setpoint by less than 1 When parameter adjustments are complete the screen returns to the normal display Figure 5 1 An additional message indicates that the stabilization period has begun During this initial stabilization period leave the door closed 5 2 Midi 40 CO2 Incubator Thermo Scientific Section6 Key Switch and Control Operation To operate the incubator with alarms activated be sure to turn the key switch all the way to the rightmost position as shown in Figure 6 1 To avoid nuisance alarms work with the key switch in the I position during the set up procedures described in Section 5 TEMP C The control panel is located on the front of your incubator 3 T 0 CO2 Before loading and using your incubator be sure to take some time 5 0 a to review the control panel functions The main display during normal operation shows cabinet temperature in degrees Celsius as measured by the sensor inside the cabinet and A UP ARROW INCREASE percentage levels of carbon dioxide CO2 When one or more of these MODE fields flashes during normal S DOWN ARROW DECREASE operation an alarm or error condition is indicated Note that ala
15. azardous materials are spilled on or inside the incubator A Thermo Scientific Section 3 Operating Standards The incubators described in this manual are classified for use as stationary equipment in a Pollution Degree 2 and Overvoltage Category II environment according to the UL3101 1 and IEC 664 standards These units are designed to operate under the following environmental conditions e Indoor use e Altitude up to 2000m e Maximum relative humidity 80 for temperatures up to 31 C e Main supply voltage fluctuations not to exceed 10 of the nominal voltage Thermo Scientific Midi 40 CO2 Incubator 3 1 Section 4 Pre Installation At delivery examine the exterior for physical damage while the carrier representative is present If exterior damage is present carefully unpack and inspect the unit and all accessories for damage If there is no exterior damage unpack and inspect the equipment within five days of delivery If you find any damage keep the packing materials and immediately report the damage to the carrier Do not return goods to the manufacturer without written authorization When submitting a claim for shipping damage request that the carrier inspect the shipping container and equipment Included Parts The following items are packaged and shipped inside the incubator g P g PP cabinet e This manual e Shelves and shelf brackets e 3 16 in 476 mm ID clear tubing for the gas connection e Cordset e 5 roun
16. d humidity pan e Additional CO sensor gasket e CO disposable filter 99 97 Midi 40 Series GO Incubator Additional Installation and Operation Manual gasket Operating manual Cordset Figure 4 1 Included Components shelves and brackets not shown Thermo Scientific Midi 40 CO2 Incubator 4 1 Section 4 Pre Installation 4 2 Set Up Location Gas Supplies Midi 40 CO2 Incubator Wiring Remove shelves from the inside of the incubator and clean the chamber Shelves and brackets can be autoclaved Install the unit in a level area free from vibration with a minimum of three inches 7 6 cm of space on the sides and rear and 12 inches 30 5 cm at the top The floor must be able to support 40 PSI single chamber incubator or 75 PSI stack of two incubators Be sure to position the incubator so that the power cord mains disconnect is easily accessible to disconnect power Do not position the equipment in direct sunlight or near any HVAC duct diffusers The ambient temperature range at the work location must be 59 to 90 F 15 to 32 C Verify that the incubator CO2 gas supplies are available near the installation area The required gas supply pressure is 15 PSI controlled by a high quality two stage regulator suitable for the connected input gas Warning Do not connect the gas at this time A Caution Connect the equipment to the correct power source Be sure to operate the incubator at the voltage sp
17. ds Only qualified persons should perform procedures associated with this symbol Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical 82 Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermofisher com Y Always use the proper protective equipment clothing gloves goggles etc Y Always dissipate extreme cold or heat and wear protective clothing Y Always follow good hygiene practices Y Each individual is responsible for his or her own safety Thermo Scientific Midi 40 CO2 Incubator iil Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to
18. e CO2 level arrives at setpoint before attempting any concentration measurement 2 Make sure that the wick in the Fyrite sample tube is moist and make sure that the tubing is in good condition no cracks or cuts 3 Hold the Fyrite upright and away from your face Depress the plunger valve briefly to vent the Fyrite and release the valve Warning Never depress the plunger valve when the Fyrite is in the inverted position Doing so causes fluid which is corrosive and contains poisonous elements to spill Refer to the MSDS sheet contained in the Fyrite instruction packet for additional information A 4 Invert the Fyrite holding it at a slight angle to drain the fluid into the top reservoir 5 Turn the Fyrite up to a 45 degree angle momentarily to allow fluid droplet drainage into the bottom reservoir 6 Hold the Fyrite completely upright and away from your face Depress the plunger valve briefly and release the valve 7 Loosen the locknut at the rear of the scale Slide the scale until the top of the fluid column lines up with zero on the scale Tighten the locknut Note When setting zero hold the Fyrite vertically and level with your eyes while sighting across the scale to the top of the fluid column Use this same procedure for adjusting zero and reading percent CO2 8 Slide the open end of the sample tubing over the sample fitting on the front of the incubator in the recessed area on the left just below the control panel
19. e offset Press Mode to revert back to normal display The factory standard method of temperature calibration uses instruments with an accuracy of 0 1 C or better To place an offset on the displayed reading to match any other calibrated temperature measuring instrument a Enter programming mode by pressing Mode and holding for 5 seconds On release the current temperature setpoint value flashes in the display b Continue to press Mode until the display changes to display Temperature offset c Use up and down arrow keys to adjust the offset d Press Mode to revert back to normal display Midi 40 CO2 Incubator 6 5 Section 7 Control Systems Theory Laboratory CO2 Incubators have a jacket between the incubator chamber and the exterior wall of the unit In water jacketed incubators the jacket is filled with water in dry wall incubators the jacket contains air The temperature control system operates in the same manner for both types of incubators Interior chamber temperature control is maintained by two sensors One sensor is located in the jacket and the other sensor is located in the chamber air Both sensors constantly signal the electronic circuitry The chamber air provides a reference point while the jacket is being controlled This circuitry recognizes that jacket temperature is very slow to react to any change in either ambient or chamber temperature but the chamber temperature can change very rapidly due to doo
20. ecified on the dataplate Incorrect voltage can result in severe damage to the equipment A Warning For personal safety and trouble free operation this unit must be properly grounded before it is used Failure to ground the equipment may cause personal injury or damage to the equipment Always conform to the National Electrical Code and local codes Do not connect the unit to overloaded power lines A The incubators described in this manual are available with the following volatages 115 120VAC single phase 60Hz 3A and 230VAC single phase 50Hz 2A Be sure to check your dataplate to verify the correct voltage for your unit Always connect the unit to a dedicated separate circuit Do not exceed the electrical and temperature ratings printed on the dataplate located near the upper hinge of the unit Electrical codes require fuse or circuit breaker protection for branch circuit conductors Use time delay fuses for 12 AWG circuits Thermo Scientific Section 4 Pre Installation C02 Gas Connections CO gas connection s are located at the rear of the unit Note The required gas supply pressure is 15 PSI Make sure the supply is available in the installation area A To install the CO2 connections complete the following steps 1 Locate the included 3 16 in 5mm interior diameter tubing 2 Cut a small length approximately 6 in 15cm from the roll of 3 16 in Smm ID tubing 3 Pull the cap off the gas connection port a
21. emperature setpoint Press Mode again The current cold alarm setpoint value then flashes in the display use Y and A to adjust it AdUstcald alarm setpoint There is a minimum deviation of 0 3 C between each alarm setpoint and the temperature setpoint Press Mode again The display changes to display CO setpoint and high and low CO alarm setpoints with the Adjust CO setpoint operating CO setpoint flashing Use Y and A to adjust it There is a minimum deviation of 1 between each alarm setpoint and the operating CO setpoint Press Mode again The current high CO alarm setpoint then flashes Use Y and A to adjust it There is a minimum deviation of 1 between each alarm setpoint and the operating CO setpoint Press Mode again The current high CO low setpoint then flashes Use Y and A to adjust it There is a minimum deviation of 1 between each alarm setpoint and the operating COs setpoint Press Mode again The display changes to display temperature offset for calibration purposes Use Y and A to adjust it Set CO offset Press Mode again The display changes to display CO offset for calibration purposes Use Y and A to adjust it Adjust CO high alarm setpoint Adjust CO low alarm setpoint Set Temp offset Service Mode service mode can be entered from Program mode by pressing Mode and Parameters holding for 5 seconds Pressing Mode repeatedly scrolls through the available functions
22. l Ze Open the inner glass door Insert a strip of paper a couple of inches wide between the door gasket and the cabinet flange and close the door Slowly pull the paper strip from the outside You should feel some resistance Repeat this test at 4 inch 10 cm intervals around the door If the door does not seal properly call Technical Services the gasket needs to be adjusted or replaced Midi 40 CO2 Incubator 8 3 Section9 Parts List 132073 132083 MIDI Door Heater 230V 132113 132121 Midi 40 CO2 Incubator 9 1 aauasioay 60 7 ASH L006 OSI UONeWJOJU AUS UE JO JOINISIP e90 INOA D IUO YSN au apisimo uonesodde jeloeds pue sonas seoueuajuiew tuonejado AjueJem jueudinba uo suonsanb noA Jamsue 0 pea 3148M 9 7 EZE OPVZ L Jo epeueo pue YSN 158y 8 y 0098 1 Je juaeuyuedag sanas jesluysa JNOA jes aseajd p nb s aonias juaudinba y SOUBUSIUIEL SAI UBAVId pue uonelado uone eysul juaudinbs 1ejap Ajjnjoseo sjenuew UOIJONJ SU Doc SaALLIe juauwdinba noA aJojeq uoneuJojur Uoneledald ous aarssuayalduos yum djey 0 Apes s BdINO s jes oway 290 INOA syonpod JO SSO JO SHJOIA SO 0 Buneja sobewep one noyjm Bulpnjoul saBewep jenuanbasuos Jo joaJpul Aue 104 ajqel aq 0u eys oway AlddV TIVHS ISOdYNMd IV INIILAVA V Y Od SSANLISA YO ALNIGVLINVHOYAW JO SAILNVYYVM ON 03IMANI YO IVYO NSLLIMM YSHLSHM SSILNVYYVM YSHLO TIV JO N3 NI ANY 3AISNT19X3 SI ALNVYYVM S
23. ol equivalent 5 milliliters per liter or O Syl in a one percent solution Disinfectants should always be diluted with sterile distilled water Caution Before using any cleaning or decontamination method except for those recommended in the manufacturer contact Technical Services to verify that you will not damage the equipment A Caution Do not use strong alkaline or caustic agents which can cause corrosion rust and pitting of stainless steel surfaces Stainless steel is corrosion resistant but not corrosion proof A Caution Do not use sodium hypochlorite solutions such as Purex and Clorox These can also cause corrosion and pitting of stainless steel A Caution Do not use steel wool pads such as Brillo they deposit carbon particles in the chamber A When cleaning stainless steel use the mildest cleaning procedure that will do the job effectively To avoid marring the surface always rub in the direction of the finish polish lines Caution Do not use aromatic solvents to clean the cabinet interior residues could cause contamination of the cabinet environment A Midi 40 CO2 Incubator 8 1 Section 8 Section title Cleaning c02 Filter Replacement 8 2 Midi 40 C02 Incubator To clean and disinfect your incubator l Lia Remove the shelves support walls and pans Clean all interior surfaces with the disinfectant solution using a clean sponge Rinse the interior surfaces at least twice with sterile distilled water
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25. r opening For example The chamber inner door is opened The chamber air sensor immediately signals a large drop in temperature but the control recognizes that unless there is a corresponding smaller drop in jacket temperature there is no need to increase the heat The system does nothing until sufficient time passes to measure how fast the chamber air temperature is rising to meet the jacket temperature after the door is closed Heat is applied to the jacket in short bursts The rate of heat application changes as the circuit monitors the two sensors This control scheme is extremely accurate and stable with the ability to control within 0 1 C of the setpoint Overtempe rature This system is activated anytime the chamber air sensor detects a Monitoring System temperature above the overtemperature setpoint which should be set no closer than 0 3 C above the chamber temperature When the system is activated the jacket heater is turned off and both audio and visual alarms are activated Control is now effectively switched to the overtemperature monitoring system The overtemperature sensor is the chamber air sensor which also provides the signal for the digital display on the control panel Thermo Scientific Midi 40 C02 Incubator 7 1 Section 7 Control Systems Theory 7 2 Door Heat System C02 Control System C02 Recovery after door opening Midi 40 CO2 Incubator Heating the inner surface of the outer door with a low wattage
26. rm conditions are displayed only O when the key switch is turned to the KEY SWITCH alarm position 4 A Figure 6 1 Key Switch Thermo Scientific Midi 40 C02 Incubator 6 1 Section 6 Key Switch and Control Operation In programming mode described in detail in Table 6 1 the main display changes to show the setpoint being viewed and related alarm setpoints For example Figure 6 2 The first value displayed is the temperature setpoint the next two values are the high temp alarm and low temp alarm setpoints The flashing value is one that Set Temp SETPOINT 37 0C 40 2C 33 8C can be changed using the up and down arrow buttons Figure 6 2 The up and down arrow buttons are used to change Programming Mode setpoint values in programming mode and for various display functions The Mode pushbutton is used to silence an audible alarm to enter P programming and service modes and in combination with the other buttons for various display functions For full descriptions of display programming and service functions refer to Tables 6 1 6 2 and 6 3 Table 6 1 Control Panel Display Function Meaning Sequence Normal operation Default display while incubator is running Display shows cabinet temperature CO2 Relative Show coldest cabinet temperature since 7 3 A Cold excursion last startup or reset Press Y Display shows cold excursion while button is pressed Show
27. t the rear of the incubator and attach one end of the tubing to the gas connection port 4 Connect the opposite end of the tubing to a high quality input gas filter 0 3 micron supplied Note that the side of the filter marked IN faces the incoming gas connection not the back of the incubator refer to Figure 1 2 3 16 in ubing Sey Gas Filter Figure 1 2 C02 Gas Connections 5 Use the remaining 3 16 in ID tubing to connect the input gas to the gas filter Keep the tubing length as short as possible to minimize the pressure drop Caution DO NOT turn on the gas supply at this time A Thermo Scientific Midi 40 CO2 Incubator 4 3 Section 4 Pre Installation Component Locations SCIENTIFIC Model Number i sensor Upper back of unit Lower back of unit WARNING For continued A protcotion against electrical shock make suro this unit Is properly grounded A CAUTION chek dataplalo for proper voltage requirements belore connection to powar 4 4 Midi 40 CO2 Incubator Thermo Scientific Start Up Prompts Power Key Switch Setpoint Parameters Thermo Scientific Section 5 Initial Start Up Procedures On first start up of the incubator the control display will go through several prompts to guide you through the entire set up process from leveling the incubator to adjusting the operating parameters During normal operation the control panel display looks
28. the display and alarm system ONLY during a power loss while the key switch is in the On or Alarm position This battery system will not power the heaters fan motor or gas control system Only qualified service technicians should replace this battery Replacement instructions and batteries are available from Thermo Scientific A A high quality two stage low pressure 15 psig 1 056 kg cm2 pressure regulator is required for proper operation of the CO2 gas supply e In line gas supply filters must be used on the CO2 supply to prevent damage to the solenoid valve s Type Microbiological Specification 0 3 micron Location rear of unit e CO2 used in the incubator must be at least 99 9 pure Midi 40 CO2 Incubator 1 1 Section 1 Introduction Specifications 12 x 18 x 14 30 5 x 46 5 x 35 3 CTN CEN ETT PS A AA CC IN to 95 37 C Weight et 60 Ibs 27kg Shipping 70 Ibs 32kg All specifications are subject to change without notice 1 2 Midi 40 CO2 Incubator Thermo Scientific Section2 Safety Precautions In this manual and on labels attached to this product the words WARNING and CAUTION mean the following e WARNING a potentially hazardous situation which if not avoided could result in serious injury or death e CAUTION a potentially hazardous situation which if not avoided may result in minor or moderate injury or damage to the equipment The following symbols are used in caution
29. warmest cabinet temperature since F Warm excursion last startup or reset Press A Display shows warm excursion while button is pressed Display shows current values alarm or error field Mode Silence audible alarm Press Mode continues to flash Reset Return to default display after Press A and V Excursion values are reset display shows current values excursion or alarm condition simultaneously Display flashes twice 6 2 Midi 40 CO2 Incubator Thermo Scientific Section 6 Key Switch and Control Operation Setpoint and Alarm To enter Program mode press and hold the MODE button until the Progra mming display indicates it is changing to Program mode Press Mode repeatedly to scroll through the available functions Exit Program mode by scrolling through all available functions and parameters using the Mode button or the display will automatically return to normal operating mode 30 seconds after the last key entry Table 6 2 Setpoint and Alarm Programming Functions Function Programming Sequence Enter programming mode by pressing Mode and holding for 5 seconds On release the current temperature Adjust temperature setpoint setpoint value flashes in the display use Y and A to adjust it Press Mode again The current warm alarm setpoint value then flashes in the display use Y and A to adjust it Adjust warm alarm setpoint There is a minimum deviation of 0 3 C between each alarm setpoint and the t
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