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PerkinElmer 2030

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1. 22 Luminosa 23 Cumnomety model een 24 ee 23 Kinetic deas Ut eie ls dio 25 Scanned Measure MENTS asien 25 Temperature Conio Laer 25 Dual Titer measuremielilssu paese ne di 25 Perk m liner 203 020050 Meares ado 26 Fluorescence OAPI ZA HOM 26 Bl uorormetry OPC OLLI B dios 28 FIuorometty bottom COUHLIDB aan os e d Ede adu eu axis und 29 Poote I sc at o o 30 Uyab ODAC cuc eta ate RE boda tatu Re ee 31 1 TNorome year elle 32 CRY APO A re 33 Appendix Calculation method for LANCE TR FRET normalization 34 AOC i Oieee ee Ede 34 COMPO UTA OU wells ai er nee 34 Normalization measurement 35 Sample Measure MEN ii 35 Y a E 36 Contents Information about user 39 SA A tons US loser aa 39 User Mai 39 Online 39 a e AR A ee en ee at iii ni dite 39 WV AN MNOS a o 40 Routine Maintenance ar nn nn GL GL 43 Cemio the Tis EFTH DU sn 43 Instruments having TR fluorometry mode 43 Dispenser MAMENANC ae A eaaet ulpa iata 43 iNeeulecmiatite arie la 44 Compactime THE database oerte a 44 Chaneme the CW amp doesnt Ne dus 45 Harndline the optical llelsucoteeoa al A mE 45 SDeCITICatllOn S indias 49 Power te quite SataII RA 49 SAIS standards lisa iii 49 Environme
2. D 613nm A 665nm Calculations 1 50 450 000 25 675 24 450 21 733 inhibition ET D 0 0543 2 50 200 000 25 050 ET 24 450 48 900 quenched ET D 0 12225 Note When using the blank corrected and normalized result as here the linearity of assay response 15 slightly over expressed For example the sample inhibiting the complex formation by 50 sample 1 gives a value which 15 only 44 from the high standard This 1s due to the fact that at the same time as the energy transfer 1s increased the donor signal 1s decreased 36 2 Information about user instructions and warnings 27 Information about user instructions and warnings Information about user instructions There are several forms of user instructions Installation instructions These are part of the instrument manual and are not normally needed by the regular user but only by the engineer who installs the PerkinElmer 2030 system However you might need them if you have to reinstall the system in another location at a future time User manual This 15 a separate manual from this instrument manual It gives information necessary for operation of PerkinElmer 2030 On line help This 15 supplied with the PerkinElmer 2030 software and can be accessed by clicking Help in any PerkinElmer 2030 window This help gives detailed information about all features of the operation which concern the normal user service information 15 not provided Rou
3. However 1f a B2 wheel 15 used instead of default type B you need to inform the system through the workstation software This 1s done by selecting Use CW lamp filter wheel B with 8 slots in Tools Options General Options General Instrument hardware Measurement technologies PerkinElmer 2030 assay result options Maximum number of assay results to be stored in the database 100 Assay results per protocol PerkinElmer 2030 Manager options Start all assay runs with Start Wizard PerkinElmer 2030 options Run Workstation in demo made no instrument needed Barcode usage mode Barcode is stored to results Platemap usage mode Assay ends after last plate in the plate map Upon assay completion new assay measurergent le automatically started using the same protocol Cancel Help Light of the selected wavelength having passed through the light guide is collimated through the sample You have the choice of using either stabilized energy mode or constant voltage mode for ensuring that the results are calculated for constant illumination In stabilized energy mode a photodiode monitors the light used to illuminate the sample and sends a signal to the CW lamp to adjust the voltage as required to maintain constant illumination In constant voltage mode the CW lamp voltage is not adjusted unlike in the stabilized energy mode A photodiode monitors the light used to illuminate the sample The change i
4. 25 Flash 25 Glow 25 Luminometry 25 38 Mains voltage 71 Maintenance 49 Models and options 8 N Normalization measurements 40 P Performance 59 Performance test 108 Photometry 19 21 34 Plate shaking 58 Plates 58 Polarization 29 108 Power requirements 57 Pump unit 102 R Red sensitive PMT 9 Repeats 58 Routine maintenance 45 49 S Scanned measurements 27 Scanning 58 Shaker option 8 Shaking 58 Shaking of plates 27 Software 59 Specifications 57 Stabilized energy mode 32 Stacker operation 10 Stackers 9 10 y Temperature 57 Temperature control 8 27 60 Time measurement 58 Time resolved fluorometry 24 37 Top counting 12 32 TR FRET 39 U Unpacking 70 USB adapter installation 81 User instructions 45 W Warnings 46
5. Minimum System Requirements e Windows XP e Intel Pentium Processor e 128 MB RAM more e 30 MB free hard disk space or more e CD ROM Drive e XGA Drive minimum setting 15 1024 by 768 pixels e Wallac Instrument Interface Board Printer Installation Install the printer according to the instructions accompanying the printer Instrument Interface Card Driver Installation The following ARCNET adapters can be used 60000569 PerkinElmer ARCNET adapter model Wallac ROG 1224 2030 PerkinElmer ARCNET adapter model USB22 CXB 65 Software installation Installation of the DriverX device ROG Note Ifthe desktop PC is ordered from PerkinElmer with the instrument this instrument interface card and the software are already installed and the instrument can be directly connected to the PC Installation of the following ARCNET card 1s described here 6000 0569 PerkinElmer ARCNET adapter model Wallac ROG for personal computers Note see the separate section for how to install the USB adapter 1f you are using 1 instead Configure the Wallac instrument interface card in the following way ON CLOSED 1 E OFF OPEN 0 Install the instrument interface card to the PC Please refer to the PC manufacturer s instructions for adding expansion cards Start the PC Once up and running you will get a message that the computer has found a new piece of hardware Note Found New Hardware Wizard may not start right after the opera
6. Note these filters are only present if the time resolved fluorometry option 15 included in instrument Homogeneous time resolved fluorometry LANCE To make LANCE measurements a correction procedure 15 required This 1s handled in the software by means of a normalization wizard as described in the user manual and help A more detailed description of how the correction 1s applied 1s given as an appendix to this chapter 22 Functional description Luminometry For PerkinElmer 2030 0020 0030 0040 Aperture slide Emission filter slide Mirror block Measurement of glow flash and dual type luminescence 15 possible with PerkinElmer 2030 In luminometry there 15 no external excitation but the light 15 produced the sample and then passes through a lens and an empty position in the emission filter slide to the photomultiplier Although one filter position in the standard slide A 15 empty and 15 intended for luminescence measurements any of the filters can be specified to be used with a luminescent sample 23 Functional description Flash type luminescence makes use of the dispenser option to initiate reactions by dispensing reagent directly into the wells of the plate See the information on the dispenser option described earlier Luminometry luminometry model For PerkinElmer 2030 0010 Emission filter slide Actual operation 1s as for normal PerkinElmer 2030 Luminometry 24 Functional description Shak
7. group Otherwise the security system of Windows prevents the correct installation of PerkinElmer 2030 Software Any end user of the PerkinElmer 2030 Software must belong either to the Administrators or Power Users group Note Do not use more than one account at the same time without logouts in Windows XP Note In Enhanced security mode the Administrator should prevent power users being able to change the system clock PerkinElmer 030 Setup Welcome to Setup Flease select one ofthe following options to continue Explore the CD ROM Exit Setup Exit all Windows programs and insert the PerkinElmer 2030 Software CD ROM into the PC the setup will start automatically If not click the setup on the CD am Setup exe The following dialogues will appear when the setup 1s started Read them carefully and follow the instructions for first time installation the default values should normally be used 80 Software installation PerkinElmer 2030 InstallShield Wizard Welcome to the InstallShield Wizard for PerkinElmer 2030 The InstallShield Wizard will install PerkinElmer 2030 on pour computer To continue click Ment InstallShield Click Next to proceed PerkinElmer 2030 InstallShield Wizard License Agreement Please read the following license agreement carefully Press the PAGE DOWN key to see the rest of the agreement SOFTWARE LICENCE AGREEMENT BEFORE USING THE SOFTWARE UR THE SYST
8. Click OK Click the Resources tab PerkinElmer ARCNET adapter model Wallac ROG Prop Resources PerkinElmer SACNET adapter model Wallac ROG Resource settings Resource type Setting W O Range ECFO ECFF 120 Range ECDO ECDF iili IR 16 Use automatic settings Change Setting Conflicting device list conflicts Cancel 71 Software installation Check that No conflicts appears The information should be as shown in the appropriate figure Close the device information display Close the device manager Close the control panel Connect the instrument to the PC Connect the coaxial cable between the instrument and the Wallac instrument interface card installed in the computer Switch on the instrument 72 Software installation Installation of the USB22 adapter Connect the USB22 adapter to the USB port you will use for the instrument connection The Found New Hardware Wizard will open other ports need separate installations Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking an your computer an the hardware installation or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search hor software CO Yes this time only Yes now and every time connect a device Click N
9. features are described separately at the end of this chapter Fluorometry top counting For PerkinElmer 2030 0020 0030 0040 CW Lamp filter wheel A Emission filter slide Mirror block 12 Functional description Inside PerkinElmer 2030 there 15 tungsten halogen continuous wave lamp 75 w spectral range 320 1000 nm This 1s used as the light source for fluorometry and photometry measurements The actual wavelength used 15 selected by means of a wheel containing up to 8 filters The filter to be used can be selected by means of the software The spectral range of the CW Lamp 15 320 1000 nm Two positions of standard 8 position filter wheel filter wheel A are used for filters of 355 and 485 nm which are specially designed for fluorescence excitation All standard filters are high quality interference filters with a diameter of 15 mm There are three empty filter positions into which customized filters can be inserted Filter Positions There are also two optional filter wheels filter wheel B and filter wheel B2 Filter wheel B left side figure has four positions for the most common size of commercial filters 1 e round one inch Filter wheel B2 right hand figure has 8 positions for filters with a diameter of 15 mm Both wheels are supplied empty but with locking rings Filter Positions Filter Positions 13 Functional description The system recognizes automatically 1f the type of wheel 15 A or
10. 2 fmol well models except 2030 detection limit 0010 polarization nM fluorescein TR Europium 6 amol well 2030 0040 0050 fluorometry detection limit Photometry Measurement 0 4 A All models except 2030 range 405 0010 0020 m UV 405 nm 0 01 A Precision lt 0 5 or 405 nm 0 01 A Measurement 2030 0030 0040 0050 absorbance range 280 nm 280 nm 0 01 A 280 nm 0 01 A detection of 10 photons s Physical dimensions Weight 46 kg without stackers 52 kg stacker model Height 354 mm 510 mm stacker model Width 493 mm Depth 595 mm Software The instrument program 15 run under Windows XP on a Pentium computer minimum 128 memory equipped with a CD ROM XGA display minimum resolution 1024 x 768 pixels 64K colours and a Wallac instrument interface card 51 Specifications Input Output connections PC coaxial ARCNET connector 93 ohm The PC must be provided with a Wallac Instrument Interface Dispenser connection Printers Connected to PC parallel port or USB Options Stackers A semi automated plate loading option including an input stacker and an output stacker 20 plates load 1420 216 or 40 plates load 1420 217 Input stacker 1420 218 Output stacker The stacker 1s designed to take plates that fulfil the following requirements Length 127 2 128 2 mm Width 84 5 86 0 mm Height 14 0 25 0 mm Ledge height 1 5 6 5 mm Barcode r
11. E This operation will take apprasimately 3 minutes Eancel Warning Do not interrupt this procedure by switching off the PC or the instrument Wait until the update is completed because an interrupt can cause permanent damage on the microcontroller boards and the only way to recover from this 15 to replace the boards ru PerkinElmer instrument flash update wizard Flash update has been completed succestully and instrument i now ready to be used Press finish ta close the program and when update 15 finished following dialogue 15 shown Click Finish to end the flash update procedure 68 Software installation PerkinElmer 2030 InstallShield Wizard InstallShield Wizard Complete Setup has finished installing PerkinElmer 2030 on your computer Installshield Click Finish to proceed Remove the PerkinElmer 2030 Software CD from the drive Continue with dispenser installation if required then do the performance test 89 Liquid dispenser installation Liquid dispenser installation 1 Unpacking Open the package and carry the instrument to its place of operation Unscrew the two thumbscrews and remove the side cover Keep the screw aside as they will be used to secure the dispenser after installation Remove the cover by unfastening the three side screws and the pressing on the two catches on the front end of the instrument see fig below PerkinElmer 90 Liquid d
12. EM YOU 45 THE CLIENT MUST AGREE WITH THIS LICENSE AGREEMENT BY PROCEEDING WITH THE INSTALLATION USAGE OR BY SIGNING OFF ON USER ACCEPTANCE TESTING WHICHEVER 15 APPLICABLE YOU ARE CONSENTING TO BE BOUND BY AND ARE BECOMING PARTY TO THIS AGREEMENT IF YOU DO NOT AGREE WITH ALL OF THE TERMS OF THIS AGREEMENT ABORT THE INSTALLATION OR USAGE PROCESS AND PROMPTLY CONTACT LICENSOR FOR INSTRUCTION ON RETURN OF THE UNUSED DEVICE S INSTALLATION MEDIA DOCUMENTATION AND OTHER RELATED MATERIAL FOR REFUND 1 DEFINITIONS Do vou accept all the terms of the preceding License Agreement If you select Mo the setup will close To install PerkinElmer 2030 vou must accept this agreement installshield Read the terms of the licence agreement then click Yes 81 Software installation PerkinElmer 2030 InstallShield Wizard Customer Information Please enter your information User Name Company Name PerkinElmer Inc Install this application for Anyone who uses this computer all users a Only for me PerkinEImer lt Back i Next gt Cancel This screenshot shows an example user name and company name Enter your own information then click Next E PerkinElmer 2030 InstallShield Wizard Choose Destination Location Select folder where setup wall install files install to this folder click Next To install to a different folder click Browse and select another folder Destinati
13. ESULTS Limits TIME RESOLVED RS RESULTS Dark counts Instrument background Sample counts Sample flashes UV PHOTOMETER Filter Sample absorbance Sample flashes TIME RESOLVED FLUOROMETER 2MM BEAM RESULTS Label Dark counts Instrument background Sample counts Sample flashes FLUOROMETER TOP COUNTING RESULTS Label Dark counts Instrument background Sample counts Normal lt 150 lt 600 gt 30000 900 1250 280 nm 1 6 2 2 A 1000 lt 450 1800 230000 2500 4000 350 3500 2500000 FLUOROMETER BOTTOM COUNTING RESULTS Label Dark counts Instrument background sample counts PHOTOMETER RESULTS Filter sample absorbance LUMINOMETER eae Dark counts Instrument background Sample counts FLUORESCENCE SEN RESULTS signal S signal P Polarization 350 10000 275000 Performance test Red sensitive FP models tube option 600 3800 730000 900 1250 280 nm 1 6 2 2 A 1000 lt 1800 lt 5400 gt 30000 2500 4000 1500 12000 7500000 1500 10000 275000 600 3800 730000 400 700 280 nm 1 6 2 2 A 1000 lt 1800 lt 5400 gt 30000 1000 2500 lt 1500 lt 12000 gt 500000 lt 1500 lt 10000 gt 75000 Test Plate Value 10 all models lt 350 lt 350 gt 10000 lt 1500 lt 1500 gt 10000 lt 1500 lt 1500 gt 10000 gt 100 000 NS gt 400 mP Performance test Sav
14. HE FILTER WHEEL Su VERTICALLY OFF FROM ITS SUPPORT REPLACE THE WHEEL AND CLOSE THE CLIP OFF FROM THE CLIPS HOLDING IT o LIFT THE LAMP VERTICALLY ZU 2 PULL THE LAMP OFF FROM CAUTION THE LAMP SOCKET DO NOT TOUCH THE OPTICAL COMPONENTS AND THE LAMP BULB OR THE INSIDE OF THE WARNING LAMP REFLECTOR WITH BARE FINGERS LAMP AND OPTICS MAY BE HOT CODE 1420 401 When you have replaced the lamp close the cover and switch power on Handling the optical filters The optical excitation and emission filters should be handled with care and touching the filter surface should be avoided If a filter is added or removed from the excitation filter wheel or the emission filter slide it should be handled at the edges only If cleaning 15 required any dust or loose particles should be first removed with dry pressurized air If necessary gently wipe the surfaces using lint free lab towels and anhydrous alcohol Use fresh towels for every wipe In a typical excitation and emission filter there 1s an arrow on the edge of the filter indicating the direction of light rays In filters with a mounting ring the direction of light 1s usually determined by the direction of the ring see fig 45 Routine maintenance Edge in the rim The direction of light The direction of light in the excitation filter wheel and emission filter slide 15 illustrated in the figures below Excitation filter The direction of
15. INSTRUMENT MANUAL March 2008 PerkinElmer 2030 Multilabel Reader gt Perkin precisely PerkinElmer 2030 Multilabel reader Valid for instruments with software version 4 0 gt PerkinElmer precisely PerkinElmer Life and Analytical Sciences Wallac Oy P O Box 10 FIN 20101 Turku Finland Tel 358 2 2678111 Fax 358 2 2678 357 Website www perkinelmer com Warning This equipment must be installed and used in accordance with the manufacturer s recommendations Installation and service must be performed by personnel properly trained and authorized by PerkinElmer Life and Analytical Sciences Failure to follow these instructions may invalidate your warranty and or impair the safe functioning of your equipment gt PerkinElmer precisely Contents Contents PerkinElmer 2030 multilabel reader ss 7 IA o 7 Modelsand ODUOHBS Sa AAA A 8 Sin le platedodels use oto at etii nee ai 9 Diae Rer mode A 9 Barcode edad opio A eee ce nds ot en ce een 10 Dispenser op OI ion onsec ii 10 Features of all models except PerkinElmer 2030 0050 12 Fluoromety OPCON a Domen umet eee 12 Eluorometry DOOM COUNTING ri 16 PRODIGI dosis ibt ds do quee dd 17 UV abSOPDA Cl ae rr ne EP p to ee 19 UV absorbance photometry model s tere t REPRE te ro et axa ned nu tute sas 20 Time stesobyed Oro ME dd 21 Homogeneous time resolved fluorometry LANCE
16. are for US62 0 COASISL BUS ARCHET ADAPTER Click Finish to close the wizard Finish Cancel Click Finish and wait until Found New Hardware Wizard opens again 74 Software installation Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software O Yes this time only CO Yes now and every time connect a device Click Next to continue This wizard helps you install software for USB2 0 amp RCNET ADAPTER p If your hardware came with an installation CD lt or floppy disk insert it now What do you want the wizard to Install fram list or specific location Advanced Click Next to continue The USB ARCNET adapter is found and software for it will be installed Select Install the software automatically and click Next gt 75 Software installation Found New Hardware Wizard Please select the best match for your hardware from the list below 5 2 ADAPTER Version Manufacturer Location ADAPTER Unknown CONTEMPORARY CONTROLS e swindows inPvoemb int SOAPTER Unknown CONTEMPORARY CONTROLS nf usb22 int This driver i not
17. cause the quality of dispensing could be affected Both the needle and the tubing will be replaced as a set It is good to check the flow of liquid dispensed during flushing If the flow 1s not normal it might indicate that the needle 15 partly blocked If this has happened due to dried reagents use water and e g ethanol to dissolve the plug If that does not help the needle needs to be changed contact Service Compacting the database You should run the PerkinElmer 2030 Database Maintenance program on a regular basis weekly or monthly depending how much you run PerkinElmer 2030 This program will compact your database and will in some situations increase the software performance drastically If you do not do this you will find that after a while your PerkinElmer 2030 software 1s not running as fast as 1t used to You can find this program by clicking the PerkinElmer 2030 icon in your Windows Start menu It is called PerkinElmer 2030 Database maintenance 44 Routine maintenance Changing the CW Lamp If the CW Lamp needs replacing lift the cover to get access to lamp Make sure the lamp 15 switched off and give the lamp chance to cool before removing it and replacing 1t Follow the instructions on the inside of cover You will probably find 1t easier to use your left hand to remove lamp from the clips holding it HOW TO REPLACE THE WHEEL AND THE LAMP ATTENTION KEEP THE LID CLOSED OPEN THE CLIP LIFT T
18. ces Manufacturer PerkinElmer Life Sciences Location PLI Slat 1 PCT bus 1 device 7 function 0 status This device is working properly IF are having probleme with this device click Troubleshoot to start the traubleshooter Device usage Troubleshoot Use this device enable w Check that you get the message that the device s working properly Click the Driver tab PerkinElmer ARCNET adapter model Wallac ROG Prop 2 lt General Resources PerkinElmer SACHET adapter model Wallac ROG Driver Provider PerkinElmer Life Sciences Driver Date available Driver Version Hot available Digital Signer Not digitally signed To view details about the driver tiles Update Driver To update the driver for this device Pall Back Driver the device faile after updating the driver roll back to the previously installed driver Uninstall uninstall the driver Advanced Click the Driver details button 70 Software installation Driver File Details HB PerkinElmer SACNET adapter model Wallac ROG Driver Files System DRIVERS drvxwdm sys Prowider Windows A 2000 DOK provider File version 5 00 2195 1 Copyright Copyright C Microsoft Corp 1881 1333 Digital Signer Mot digitally signed k Check that the driver shown in the picture is the one in use on your system
19. counting modes included depend on the model Fluorescence 320 700 nm emission up to 850 nm optional Bottom fluorescence 320 700 nm up to 850 nm optional Time resolved fluorometry TRF LANCE Fluorescence polarization Dual window TRF Glow luminescence Flash luminescence Dual luminescence Photometry 320 1000 nm UV absorbance 230 320 nm Dual excitation fluorescence Dual emission fluorescence Scanning Fast and slow kinetic measurements 53 5 WEEE instructions for PerkinElmer products WEEE Instructions for PerkinElmer Products A label with a crossed out wheeled bin symbol and a rectangular bar indicates that the product is covered by the Waste Electrical and Electronic Equipment WEEE Directive and is not to be disposed of as unsorted municipal waste Any products marked with this symbol must be collected separately according to the regulatory guidelines in your area The objectives of this program are to preserve protect and improve the quality of the environment protect human health and utilize natural resources prudently and rationally Specific treatment of WEEE is indispensable in order to avoid the dispersion of pollutants into the recycled material or waste stream Such treatment is the most effective means of protecting the customer s environment Requirements for waste collection reuse recycling and recovery programs vary by regulatory authority at your location Contact your local resp
20. d in the same windows to obtain the uncorrected results D and A These results are then corrected using the following equation to give the final result Ra Where A Bl c x D x Dax D A 1s the fluorescence intensity of the sample in the acceptor window D 15 the fluorescence intensity of the sample in the donor window c and Dmax are obtained from the configuration A Bl c x D ET the actual energy transfer from the donor to the acceptor 35 Functional description If there 15 quenching in the sample it will affect both the A and D measurements The ratio Dax D normalizes the result to the maximum intensity value and eliminates the effect of quenching in the sample This can be seen in the following numerical example Example The three normally three with duplicates first wells contain the configuration samples D 613 nm A 665 nm Calculations Calculations Ra 500 000 1350 c E High standard 400 000 50 000 ET max 48 900 u 900 Dax A max ET Dus x 0 12225 The following table gives the corrected values calculated for two samples The first has a 50 9o lowered complex formation and is thus a true positive The second sample 1s quenched by 50 and without further correction appears as a false positive I e the apparent energy transfer in each case 1s the same 24 450 cps The normalization Dmax D reveals the true situation showing the first result to be a true positive and the second not
21. digitally signed Tell me why driver signing i important Select usb22 inf and click Next gt Hardware Installation The software vou are installing for this hardware 5 2 ADAPTER has not passed Windows Logo testing to verify its compatibility with Windows XP Dell me why this testing ls Important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway Click Anyway 76 Software installation Files Needed The file LISBZ2DRV SYS on Unknown is needed Cancel the path where the file is located and then click Copy files from Browse Browse the Support Driver USB22 folder for the USB22DRV SYS file Locate File Look in O t e m File name usbzzdrv sus w Open Files of type ISB22DAY SYS USB22DAY SY Cancel Files Needed The file LISBZ2DRV SYS on Unknown is needed Cancel the path where the file is located and then click Copy files from DAsuppor Driven LB v Click OK to copy the file 77 Software installation Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing
22. e is used Fuses T3A Mains 110 120V Fuses T 1 6 A Mains 220 240V Its purpose 15 to alert you to fact that fuse holder 15 set 220 240 be connected to you must change the voltage selector To do this pull out the voltage selector change the fuses turn it round and replace it See the pictures on the next page Make sure you use the correct fuses i If this setting is not suitable because of the voltage of the mains the instrument is to e connect the mains cable e connect the ARCNET cable to port A 63 Instrument installation e check that the terminator is properly fitted into port B Mains 220 240V TO WALLAC INTERFACE T CONNECTOR TERMINATOR 75RBNC T 93 R BNC 1186 0014 1186 0015 ARCNET FUSES T 3A Mains 110 120V ARCNET CABLE FUSES T 1 6A Mains 220 240V RG 62 93 ohm 3m 150 VA 50 60 Hz RE TE mes POWER TERMINATOR Ba 1186 0015 A0SZ AINO ASN 220 240V 220 240V Mains 110 120V TO WALLAC INTERFACE t EN T CONNECTOR TERMINATOR 75R 93 R BNC 1186 0014 1186 0015 ARCNET FUSES T 3A Mains 110 120V ARCNET CABLE FUSES T 1 6A Mains 220 240V RG 62 93 ohm 150 VA 50 60 Hz A 4 5m 1085 9032 POWER lt B Em TERMINATOR USE A 93 R BNC FUSES EMPLOYER 1186 0015 v v 110 120V 64 Software installation Software Installation
23. e pump unit into position as indicated in the picture below before securing it to the dispenser unit with screws 2x NA screws 2 wf age rl E Te DELETE O Slide pump unit Connect the tubes 2x to the pump unit as indicated in the pictures 95 Liquid dispenser installation E 43 Before installation After installation 96 Dispenser calibration 5 Dispenser calibration Equipment needed 96 well solid clear plate 1086 3400 Adjustment tool for dispenser arm Horizontal angle of the dispenser arm With the power switched off open the top cover of the dispenser and check the arm by moving 1t manually in and out Check that the arm does not scratch the upper part of the measurement head and that the space between the arm and the upper part of measurement head is 1 2 mm Note If arm 15 too low 1t will hit against the measurement plate during normal use and the dispenser needle s might be damaged Adjust 1 2nm Wallac 1420 Measurement Head NHL Adjusting Directions Once the arm looks OK manually switch the system on and go to the next step Measurement position of the dispenser arm Check the position of the arm in the following way Load and initialize the service program and run the arm to the measurement position Insert the adjustment tool and click OK then run the arm to the measurement position by clicking Gololurrent 97 D
24. eader 1420 221 Barcodes that can be read EAN UPC 2 of 5 interleaved CODEBAR CODE39 CODE128 Temperature control The temperature of the measuring chamber can be regulated from ambient 2 C to 50 C The PMT is isolated and kept at 25 TR fluorometry 1420 110 The TR fluorometry optics are based on a xenon flash lamp The counting delay can be set from 0 to 65515 s Included in this option are flash absorbance and dual window TR fluorescence counting Expanded wavelength range to IR 1420 113 The wavelength range 1s expanded up to 850 nm Dispenser options l channel Dispenser 1420 2550 2 channel Dispenser 1420 2560 3 channel Dispenser 1420 2570 4 channel Dispenser 1420 2580 From 1 to 4 channels can be directed to a single well depending on the option used The default volume range 15 5 350 uL in 1 uL increments 22 Specifications Accuracy typically 5956 for 5 uL 0 59 for 50 uL 0 05975 for 350 uL Precision typically lt 1 4 for 5 uL 0 290 for 50 uL 0 0275 for 350 uL Speed minimum and maximum speed are related to the volume Dead volume 0 5 mL Tubing Inner diameter 0 7 mm Outer diameter 2 0 mm Material PTFE Enhanced security mode The Enhanced Security mode 15 intended for facilities that have to comply with the 21 CFR Part 11 regulation from the Food and Drug Administration FDA of the USA If required it must be selected during installation Counting modes Note
25. ext to continue Select for Windows Update connection and click Next gt The installation requires Found New Hardware Wizard to be run twice The first time a generic USB Device 15 found Found New Hardware Wizard This wizard helps you install software for USB Device If your hardware came with an installation A floppy disk insert it now What do pou want the wizard to Install from a list or specific location Advanced Click Next to continue 73 Software installation Insert the 2030 Software CD into the drive select Install the software automatically and click Next gt While installing the software the following dialog will appear Hardware Installation The software you are installing for this hardware USB2 0 BUS SACHET ADAPTER has not passed Windows Logo testing to very its compatibility with Windows XP me why this testing ls important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway Click Anyway Found Mew Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the softw
26. filter and other parameters can be specified for dual label measurements In this case the system rapidly changes the filter and immediately makes the second measurement for the same well with no adjustments needing to be made in between 25 Functional description PerkinElmer 2030 0050 features This model allows fluorescence polarization measurements in addition to the technologies available in other models of PerkinElmer 2030 Fluorescence polarization requires a somewhat different internal design so new pictures are shown here and the differences noted Fluorescence polarization For PerkinElmer 2030 0050 CW Lamp filter wheel A Aluminium reflector Polarization emission filter slide 26 Functional description Light 15 produced by the same tungsten halogen continuous wavelength lamp used for normal fluorometry and photometry In fluorescence polarization the excitation energy control is only by means of the constant voltage mode The lamp 1s run at full power with no voltage adjustment Light from CW lamp 15 directed via a light guide through a polarization excitation filter slide This has two positions with small and normal apertures You can select which 1s of these 1s to be used The filter polarizes the light in the S plane The polarized light 1s then directed by means of a mirror block onto the sample The polarized emission light passes back through the mirror block and through a polarization emission f
27. fluorometry For PerkinElmer 2030 0040 Emission filter slide Flash excitation filter slide 21 Functional description This configuration 15 used for both types of time resolved fluorescence measurements 1 e DELFIAS involving dissociation and enhancement and LANCE homogeneous assays For enhanced performance with LANCE it 1s possible to install an optional red sensitive detection unit PMT In time resolved fluorometry excitation light is produced by a UV xenon flash lamp spectral range 230 400 nm The light passes through an excitation filter fitted into a slide This slide 15 not accessible to the user unlike the CW Lamp wheel and emission filter slide where filters or the whole wheel or slide can be changed A lens and mirror system 1s used to direct the light through the sample well Emission light from the sample then passes through a lens and filter system to the photomultiplier just as in the fluorescence case Both excitation and emission light are directed from the top of the well If the adjustable beam size option 1s installed then there 1s an additional flash excitation lens slide Two positions of the standard 8 position emission filter slide A are used for emission filters suitable for labels of rare earth chelates 1 e Europium 615 and Samarium 642 Both these filters are high quality interference filters with quite narrow 6 8 nm bandwidths and exceptionally high peak transmissions gt 70
28. formed can be either Fill Empty or Flush you can select the operation you wish to perform This 1s then performed on all the selected pumps Fill is used for filling the syringe and tubing before operation begins Empty 15 used after operation ends to empty the contents of syringe and tubing back into the bottle Flush 15 used for cleaning syringe and tubing after 1t has been emptied Note before performing this operation make sure the tubing from each dispenser has been put into water as described earlier Take the tubing out from the water Empty any water remaining in the tubing by clicking Fill This draws air into tubing and expels any water drops Fit the end of the tubing into the reagent bottle Click Fill to fill the tubing with reagent Needle maintenance Every time the dispenser has been used the tubing must be flushed properly When this 1s done the needles are rinsed at the same time However it 1s good to regularly clean the outside of the needles Avoid touching the needles with your fingers To clean the needles use e g a stick with a tip of soft material dipped in ethanol or use a spray bottle filled with ethanol Be very gentle when touching the needles so that they are not bent out of shape Rinse with water The position of the needles 1s very critical for high quality performance If a needle 1s bent call the service engineer to get the needle replaced Do not try to re adjust the damaged needle be
29. h as stacker dispensers shaker temperature control top or bottom counting and scanning The software is a 32 bit application running under Windows XP on a PC connected to the instrument Plates with up to 1536 wells can be counted as well as Petri dishes slides filters and Terasaki plates Plates can be loaded manually or with stackers for automatic operation Output can be to a file on the PC and or to a laser printer PerkinElmer 2030 has an optional Enhanced Security mode intended for facilities that have to comply with 21 CFR Part 11 regulation from the Food and Drug Administration FDA of the USA During installation you can select if you want to use the Enhanced Security mode This mode is described in the User manual Functional description Models and Options 2030 0010 dedicated luminometry model for luminometry only 2030 0020 fluorescence and luminescence model for fluorometry and luminometry 2030 0030 photometry model for fluorometry luminometry and photometry including UV absorbance 2030 0040 time resolved fluorometry model for fluorometry luminometry photometry including UV absorbance and time resolved fluorometry 2030 0050 all five leading technologies model for fluorometry luminometry photometry including UV absorbance TR fluorometry and fluorescence polarization All models include temperature control and shaker operation Functional description The following options are available fo
30. hanced Security installation code in case you need it in the future When you have given the installation code click Next PerkinElmer 2030 InstallShield Wizard Select mode Select made that suits pour needs Normal use with an instrument Demo use without an instrument Installs Cancel Click Next 1f your 1s connected to the instrument With no instrument workstation can be run demo mode and the software itself simulates the instrument In that case select Demo and click Next verre 2030 InstallShield Wizard Start Copying Files Review settings before copying files Setup has enough information to start copying the program files IF you want to review ar change any settings click Back If vou are satisfied with the settings click to begin copying files Current Settings Setup type Custorn Destination directory C Program Files PerkinE mer PerkinE 030 Selected mode Normal mode installshield lt Back Cancel 84 Software installation If all settings are OK click Next PerkinElmer 2030 InstallShield Wizard Setup Status Writing system registry values InstallShield Wait until the setup 1s completed If you have selected Enhanced security mode the following two dialogues will appear before the flash update procedure begins If you have not selected it the software installation will continue directly with
31. hich are identified by the barcode reader This identification can be by plate number or protocol number In the former case the protocol to be used for the measurement must be specified In the latter the barcode identifies the protocol to be used for that plate This system is especially useful for the stacker models Dispenser option The optional dispenser allows measurements to be made that require e g the addition of reagent to start the process as in the case of flash luminescence or start and stop an enzyme reaction It can also be used for dispensing Enhancement Solution for a DELFIA time resolved 10 Functional description fluorometry measurement It can be used with any of the technologies supported by PerkinElmer 2030 The dispenser can have up to four pumps fitted This allows separate pumps to be dedicated for specific purposes All the needles are directed to the same well allowing dispensing of more than one reagent to the same well The number of pumps fitted and the total volume of each pump are set during installation This information then appears when dispenser maintenance 1s selected The default setting for the dispensed volume can be between 5 to 350 microliters The optional dispenser 15 a separate unit from PerkinElmer 2030 itself making for convenient maintenance and flexibility in syringe selection 11 Functional description Features of all models except PerkinElmer 2030 0050 Note PerkinElmer 2030 0050
32. ilter There are two of these in a slide One filter is polarized in the S plane and other at a right angle in the P plane Finally the light passes through an emission filter and then to the PMT One measurement is made with the S emission filter slide and the other with the P emission filter slide The final result 1s calculated by combining these measurements in the formula S G P Polarization mP 1000 S G P Where 5 and P are the measured results with S and P emission filters respectively G 1s a factor to correct for the effect of emission filter transmission variation and sample viscosity See the PerkinElmer application note on Fluorescence polarization assays for more details 27 Functional description Fluorometry top counting For PerkinElmer 2030 0050 CW Lamp filter wheel A Aluminium reflector Polarization emission filter slide excitation filter slide This functions the same as in normal PerkinElmer 2030 28 Functional description Fluorometry bottom counting For PerkinElmer 2030 0050 CW Lamp filter wheel A Emission filter slide Reference Channel Emission Channal Polarization emission filter slide Mirror block This functions as in normal PerkinElmer 2030 29 Functional description Photometry For PerkinElmer 2030 0050 CW Lamp filter wheel A Aluminium reflector 29 Mirror block This functions as in normal PerkinElmer 2030 30 Func
33. ilters 340 260 and 280 nm 49 Specifications Detection units Photometry Photo diode photometric range 320 1000 nm Fluorometry luminometry and optional TR fluorometry 1 Photomultiplier tube R 1527 optional R4632 2 Emission filter slide A provided with 8 filter positions 025 4mm with the following filters 535 nm and 460 nm Changeable emission filter slide B provided with 8 filter positions 25 4mm Optional filters 615 nm 642 nm Plates 1 to 1536 well plates are compatible with the instrument Note for luminometry and bottom reading well densities above 384 are not recommended The user can define non standard plate configurations The maximum outer dimensions are 86 0 x 128 2 x 25 mm For Terasaki plates and petri dishes optional cassettes are available Minimum plate height 1s 4 0 mm Both opaque and clear plates are suitable for photometric measurement a clear bottom 15 required Plate shaking Three plate shaking modes are available linear orbital and double orbital Three speed levels can be selected and the amplitude of the movement is adjustable Scanning scanning of wells several measuring points within a well are available for all technologies Repeats Repeated measurements or operations can be defined by plate strips or well Measurement time Detection time per well 1s software selectable between 0 1 600 seconds 50 Specifications Performance Fluorometry Fluorescein lt
34. ing of plates The plate conveyor also acts as a shaker You can specify how long how fast and with what amplitude the plate 1s shaken A further feature 1s that the mode of shaking can be linear orbital or double orbital figure of eight Shaking occurs when the conveyor has moved the plate to the measuring position Kinetic measurements For reactions taking time repeat measurements are necessary PerkinElmer 2030 allows these kinetic measurements to be made The number of repeats up to 300 can be specified and the time between repeats up to 3600 s These fast kinetic measurements can only be made 1f well mode 15 selected Slow kinetic measurements can also be made using plate repeats Scanned measurements Measurements can be made at different points of a well for samples in which light output 1s not homogeneous e g in cell culture wells The number of spots to be measured can be defined Up to ten spots can be measured in both the horizontal and vertical directions maximum of 100 points altogether The distance between spots can be set The distribution of the measurement spots can be square or rounded Temperature control The temperature control option maintains plates at a temperature specified by the user This can be between room temperature 2 C and 50 C The measuring chamber itself is heated so no special plate holder is needed The PMT tube environment is maintained at 25 Dual filter measurements A second
35. ing the EEPROM parameters to the hard disk Save all EEPROM parameters on the hard disk The parameters can be restored if the LCC C board s has to be replaced Eeprom gt ALL gt Parameters 2 Save the current eeprom parameters of the selected groups to the hard Click Yes Warning AN Current hard disk values will be Click OK im NODES AND PARAMETER GROUPS HODE GROUP STATUS INSTRUMENT DATE Hode 1 ZYCONV saved 4200369 1999 09 22 10 24 37 LOADER saved 1200369 1999 09 22 10 24 40 LIFTS saved 4200369 1999 09 22 10 24 41 HAL 0F saved 4200369 1999 09 22 10 24 42 Hode 2 HEASER saved 4200369 1999 09 22 10 24 44 Hode 3 FLASHE saved 4200369 1999 09 22 10 24 47 DISPER saved 4200369 1999 09 22 10 24 48 TEMPER saved 4200369 1999 09 22 10 24 50 mim 7 102 First time start up First time start up The first time you start the software after installation click your Windows Start button Select programs and from that menu PerkinElmer 2030 Select PerkinElmer 2030 Workstation as shown in the picture PerkinElmer 2030 A PerkinElmer 2030 Database Maintenance 2 PerkinElmer 2030 Readme File PerkinElmer 2030 Service Program PerkinElmer 2030 Workstation yt The following dialogue will appear PerkinElmer 2030 Instrument Server This instrument has nok been used with this workstation before ue dU Press Yes bo add the serial number of Ehe instrume
36. ions and check box Search removable media should be selected Click Next The driver will be found and installed 67 Software installation Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for PerkinElmer SACHET adapter model Wallac ROG Click Finish to close the wizard Cancel Click Finish to close the wizard ARCNET card device check BT AS Click Start 2narea L OCUMENIES Control Panel Select Control Panel Vig Control Panel Switch E me View From the control panel select Switch to Classic View System Then select the System icon 68 Software installation A System Properties System Restore Automatic Updates Remote General Computer Name Advanced Spaten Select the Hardware tab Iz LAE VIL IgE LO vp INC t Im Lc c lt lt lt na ES Click the Device manager button 4 DHKDHSOJ H Y Computer Disk drives E E Display adapters Drivers devices D Eg PerkinElmer ARCNET adapter model Wallac RO 4 DYD CD ROM drives Y Floppy disk controllers A q disk drives Check that the correct driver 1s installed Double click on the driver 69 Software installation PerkinElmer ARCNET adapter model Wallac ROG Prop General Driver Resources PerkinElmer SACNET adapter model Wallac ROG Device type Divers devi
37. ispenser calibration Now the arm goes to the measurement position Check that the horizontal angle 15 OK Click GoToCurrent a couple of times and check If everything 15 OK with the adjustment tool mirror check the position of the needle s Calibrate the needle s to the center sideways with right or left buttons and the in amp out position by adjusting with the adjustment screw see fig below TIGHTENING SCREWS UNDER THE FRAME ADJUSTING SCREW HORIZONTAL DIRECTION Once the needles are in the center click Save and GoToCurrent for a last check Remove calibration tool and make the following test of the dispenser Dispenser test Fill the pump s with water by clicking the prime button twice Use a 96 well solid clear plate for the test Test each pump separately Dispense 50 ul well into the first strip speed 4 If there 15 water outside the well or on the walls calibrate the arm again If the water 1s dispensed into the wells normally dispense twice to the whole plate 100 ul well speed 4 Check the result 98 Final installation Final installation After calibrating the arm install the PerkinElmer 2030 cover and secure with the side screws 3x Slide the dispenser against the PerkinElmer 2030 Ensure the dispenser fits into the grooves on the side Tighten dispenser unit to PerkinElmer 2030 with screws 2 THE 11110 Adjust the height of the dispenser by turning the feet of dispense
38. ispenser installation 2 Setting the robotic arm Open the lid of the dispenser unit The robotic arm block 1s attached to the dispenser unit with a metallic protection plate and the arm 15 locked with a cable during transportation Remove the robotic arm from the dispenser unit by removing the cable tie and protection plate as indicated in the figure below Cable tie Check that the robotic arm 15 perpendicular to the base by using a try square or an engineer s square as indicated in the next figure Ensure that they are at a right angle 90 to each other If they are not perpendicular loosen 2x and adjust 1x the screws on the base see the figure below Turning it clockwise will adjust robotic arm downwards while turning it anti clockwise will adjust the robotic arm upwards Liquid dispenser installation Once the robotic arm 1s perpendicular secure 1t to PerkinElmer 2030 see fig below by means of screws 3x Adjust the height of the arm before tightening them for detailed instructions please see below Attach and secure arm with the three screws To adjust the height of the robotic arm locate the arm to a couple of millimeters away from PerkinElmer 2030 and adjust the screws for the guide pins so that the height between PerkinElmer 2030 and the arm is 1 1 5 mm Push the arm completely into the instrument and check that it moves freely without touching the measurement head on the way in Adju
39. light Excitation filter wheel The direction of light Emission filter slide mH 46 4 Specifications Specifications Specifications Power requirements PerkinElmer 2030 Power consumption 400 VA Mains voltage 110 120 V 220 240 V 50 60 Hz Liquid Dispenser Power consumption 200 VA Mains voltage 100 240 V 50 60 Hz Safety standards The instrument 1s designed to meet the following safety standards 1 EN61010 1 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements 2 EMC Directive ENS0082 1 immunity and 50081 1 EN61000 3 2 emission Environmental conditions Temperature 15 35 C Relative humidity 10 85 Light sources Continuous light source for fluorometric and photometric measurements 1 Tungsten halogen lamp 75W lifetime gt 3001 Spectral range 320 1000 nm 2 Rotatable filter wheel A provided with 8 filter positions Y 15 mm Standard high quality interference filters 405 nm 450 nm 490 nm 355 nm not for fluorescence polarization 485 nm Changeable rotatable filter wheel B provided with 4 filter positions 25 4 mm Changeable rotatable filter wheel B2 provided with 8 filter positions O 15 mm Optional flash light source for TR fluorometric measurements 1 UV xenon flash tube L4642 or equivalent spectral range 230 400 nm 2 Filter slide provided with 3 filter positions 022 4mm F
40. lter type A The size 1s round one inch The excitation emission pairs with wavelengths 355 460 and 485 535 are used for e g umbelliferone and fluorescein respectively Note when the properties of a label are defined then the filters to be used for measurements with that label are specified When a protocol 15 selected for a measurement the appropriate filters are moved into position automatically 15 Functional description Fluorometry bottom counting For PerkinElmer 2030 0020 0030 0040 CW Lamp filter wheel Emission Channal 16 Functional description In the case of bottom counting of prompt fluorescence samples excitation light 15 directed through a light guide underneath the plate Emission light from the bottom of the sample goes back along the same light guide to the optical block where the emission filter 1s and from there to the PMT Bottom counting allows measurements to be made for samples in which cells are at the bottom of the wells or there are lids on wells Switching between top and bottom counting 15 done by software and both methods can be used for the same plate Photometry For PerkinElmer 2030 0020 0030 0040 gt gt CW Lamp filter wheel A Mirror block 17 Functional description For absorbance measurements the same excitation system 1s used as for prompt fluorescence The wavelength of the light can be in the range 320 1000 nm There are three positions i
41. n filter wheel A that are fitted with filters for the most common absorption wavelengths 1 e 405 450 and 490 nm In absorption measurements light passes through the well to a photodiode beneath the sample plate The intensity of the filtered and stabilised beam 1s first measured without any sample and then the samples in one plate are measured The absorbance value 15 then calculated from the equation log I Io where I 1s the light intensity measured without any sample a reference beam and I 1s the intensity after an absorbing or reflecting medium Because the optical surfaces of the empty well reflect backward about 8 of optical energy the measured absorbance values are always about 0 04 A for a empty clear plate 18 Functional description UV absorbance For PerkinElmer 2030 0040 and 0050 excitation filter slide For UV absorbance measurements the same excitation system 1s used as for time resolved fluorescence The wavelength of the light can be in the range 230 320 nm There are two fixed positions in the flash excitation filter slide that are fitted with filters for the UV absorption wavelengths 1 e 260 and 280 nm The measurement is as for normal photometry 19 Functional description UV absorbance photometry model For PerkinElmer 2030 0030 Flash excitation filter slide The operation 1s as for normal PerkinElmer 2030 UV absorbance 20 Functional description Time resolved
42. n the signal from this photodiode 15 used in the software to correct for changes in lamp intensity so as to ensure that the results are calculated for the same illumination In constant voltage mode it is recommended that the lamp 15 run at full power Constant voltage mode 15 recommended when counting kinetic dual excitation fluorescence labels 14 Functional description In both modes the energy range 1s user adjustable to help find the optimum linear range for samples being measured and thus keep the output signal within the linear range of the instrument In top counting the sample 15 illuminated from above and then the fluorescence shines up from the sample and passes through a mirror slide a lens system and an 8 position emission filter slide to a low noise photomultiplier spectral range 400 700 nm equipped with fast single photon counting electronics There is also a combined shutter and aperture slide In the standard emission filter slide A there are two positions for emission filters of conventional fluorescence at wavelengths of 460 and 535 nm Filter Positions All Filters can be dragged fram the amp vallable Filters list to empty position in Ehe slide from position to an empty or back to the list 000000009 Emission filter slide A can be replaced with another one which has no predefined filters so you can choose which filters you want to load There are eight positions the same as in fi
43. nitors BB Network adapters E PerkinElmer instrument adapters Y Ports COM amp LPT 848 Processors Sound video and game controllers System devices Universal Serial Bus controllers Generic USB Hub Intel R 82801GB USB Universal Host Controller 27C8 Intel R 82801GB USB Universal Host Controller 27C9 Intel R 8280198 USB Universal Host Controller 27 Intel R 82801GB USB Universal Host Controller 27CB Intel R 82801GB 1582 Enhanced Host Controller 27CC USB Root Hub USB Root Hub LISB Root Hub LISB Root Hub USB Root Hub LISB2 0 ADAPTER Right click to see the properties of the adapter Check the device status USB 2 0 ARCNET ADAPTER Properties General Driver Details ce USB2 0 ARCHET ADAPTER Device type Universal Serial Bus controllers Manufacturer CONTEMPORARY CONTROLS Location Location 0 LISBZ2 Device status This device iz working properly IF you are having problems with this device click Troubleshoot to start the troubleshooter Troubleshoot Device usage Use this device enable bul Connect the instrument to the PC Connect the coaxial cable between the instrument and the Wallac instrument interface card installed in the PC Switch on the instrument and the PC 79 Software installation PerkinElmer 2030 Software Installation When installing PerkinElmer 2030 Software you MUST belong to the local Administrators
44. nt bo the database or press to cancel instrument boot Click Yes The workstation software will then start up normally See the user manual for operation information 103 T Index 105 106 Index 2 21 Part 11 7 61 A Absorbance 19 21 ARCNET adapter installation 74 USB installation 81 B Barcode reader 9 60 Barcode reader option 10 Barcodes 60 Bottom counting 18 33 C Cleaning 49 Configuration samples 39 Connections 60 Constant voltage mode 32 Continuous wave lamp 13 Counting modes 61 CW Lamp 13 D Database maintenance 50 DELFIA 24 37 Dimensions 59 Dispenser 9 Calibration 105 Test 106 Dispenser installation 98 107 Index Dispenser maintenance 49 Needle 50 Dispenser option 10 Dispenser options 60 Dual filter measurements 27 E Emission filter slide 16 Enhanced security 61 Enhanced security mode 7 9 91 F Fill 50 Filter wheel 14 Filters 13 Cleaning 51 Handling 51 Mounting 52 Fluorescence polarization 28 Fluorometry Bottom counting 18 33 Top counting 12 32 Flush 50 H Help 45 Homogeneous TRF 24 Humidity 57 Installation 69 Software 73 Installation instructions 45 Index Instrument features 7 K Kinetic measurements 27 L Lamp 57 Changing the CW Lamp 51 CW 13 29 Flash 24 Spectral range 13 LANCE 24 37 LANCE calculation 39 LANCE normalization 24 39 Luminescence Dual
45. ntal Conditions eo St e cp es 49 ES hb source seat 49 D CORS ei c O neo miel 50 A EUM EID IEEE AMI MEME I IM MM EI ere eT en 50 HA E rer Dee tee 50 SCAN IIND ee er are 50 REPASA tint te ee 50 MEA NA 50 P HOTMANC 51 POC star eu 51 UI e MEM ME 51 Input Output CONNECTIONS nei 52 DOG ONG igen 52 A bs smn 32 Barcode reader IPOD Tee 52 Temperature CONTO 52 TRIO ROME 4 E420 VW see tr a int 22 Expanded wavelength range to IR 1420 113 52 Dispebset OPO 52 Enhanced Securtby dO 53 COMUNA roto C 53 Contents instrument Installation 61 ee ee 61 Er 61 ES 62 Removing Transport Protection Piece ss 63 Check h MANS voltase soine ira 63 Software Installation 65 Nr System Reg UNC Mei reinen 65 Printer ac A oi tic ce tie A 65 Instrument Interface Card Driver Installation ss 65 Installation ot the Driver device ROG de uk emule 66 ARCNET card device cheek seen ea ti 68 Connect the Instrument tothe PC eek 12 installation ot 20 iia 73 Connect the 1nstrument tote RC iia 79 PerkinElmer 2030 Software Installation anni eas eie eoi testes RE 80 A O E E E A II A A 80 Liguid dispenser installato 90 MO AC AAA TE 90 A A 91 3 Blec
46. on Folder C Program Files PerkinE Imer Perkin Imerz 30 Browsen Cancel installshield If the proposed destination folder is OK click Next Otherwise click Browse and select a new destination folder When you have done this then click Next 82 Software installation PerkinElmer 2030 InstallShield Wizard Select Features Select the features setup will install Select the features you want to install and deselect the features you do not want bo install w Workstation software ge Result and protocol database 21 CFR Part 11 support 103 48 MB of space required an the drive 16679 17 MB of space available on the C drive installshield lt Back Nest gt Cancel Select the features you want to install then click Next The Result and protocol database option 1s selected by default and greyed If you have not selected Enhanced security mode the next dialogue to appear 1s the Select mode dialogue see the screen shot at the bottom of the next page If you have selected Enhanced security then you must give the Enhanced security installation code as shown in the following screenshot You will find this number on a sticker with the software CD PerkinElmer 2030 InstallShield Wizard Enhanced Security Please enter Enhanced Security installation code 2030 3030 lt Back Next gt Cancel 83 Software installation Note Make sure you save the En
47. on samples can be included on the plate and run at the same time as the assay samples instead of using the wizard In LANCE TR FRET assays the sample interference can be corrected from the time resolved acceptor signal A based on the fact that the energy transfer signal 1s a direct function of the excited states of donors in the complex This traditional ratio measurement 1s improved by subtracting both blank Bl and donor crosstalk c x D where D 15 the donor signal values from the time resolved integral of acceptor signal A before calculating the ratio The corrected values are defined as a blank corrected normalized ratio R Use of the equation requires a configuration plate or wells where background due to the plate blank and the contribution of the donor to the signal in the acceptor window can be measured Maximum energy transfer samples are also measured on the plate to allow actual sample measurements to be normalized to the maximum donor value and thus eliminate the effect of any quenching that there might be in the sample Note that all measurements have to be done with identical instrumental parameters with the exception of filters for donor and acceptor This means that the microplate buffer reagent concentrations and volumes should be the same as those to be used in the actual sample measurements Configuration wells Dispense the configuration samples preferably in duplicate 1 Blank control Colourless pure as
48. onsible body e g your laboratory manager or authorized representative for information regarding applicable disposal regulations Contact PerkinElmer at the web site listed below for information specific to PerkinElmer products Web address http las perkinelmer com OneSource Environmental directives htm Customer Care 1 800 762 4000 inside the USA 1 203 925 4602 outside the USA 0800 40 858 Brussels 0800 90 66 42 Monza Products from other manufacturers may also form a part of your PerkinElmer system These other producers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive Please contact these producers directly before discarding any of their products Consult the PerkinElmer web site above for producer names and web addresses gt PerkinElmer precisely 57 56 6 Installation information PerkinElmer 2030 Installation Instructions Liquid Dispenser Installation Liquid Dispenser Calibration Performance Test 59 60 Instrument installation Instrument Installation Environment Although normal clean laboratory conditions are usually quite satisfactory as an operational environment 1t 1s useful to take the following points into consideration Ventilation the room should be adequate for all conditions of use the temperature should be reasonably constant at about 2 relative humidity should not be excessive direct s
49. r all models 1420 221 Barcode reader 1420 2550 1420 2580 One dispenser 4 dispensers 2030 3010 Enhanced security mode software option 2030 1010 Stacker option 1420 113 Red sensitive PMT option is available for all models except 2030 0010 1420 110 The time resolved fluorometry option can be fitted in the field to 2030 0020 and 2030 0030 1420 3050 WorkOut 2 5 Data Analysis software 15 included in models 2030 0030 2030 0040 and 2030 0050 as standard and 1s available as an option for other models Single plate models In the models without stackers a single microplate 1s loaded into the reader Stacker models Functional description In the stacker operation plates are loaded into the input stacker with the first plate to be measured at the bottom The empty output stacker 15 fitted first into the multilabel reader 1t occupies the left hand position Then the input stacker with plates 15 fitted in the right hand position During operation the plates are moved one by one from the input stacker to the measurement position After each plate has been measured 1t 15 moved to the output stacker When measurement 15 complete the output stacker can be removed A quick release mechanism allows the plates to be emptied easily from the stacker Note stacker model can also be used as a single plate model if required Barcode reader option An optional barcode reader can be included This allows loading of barcode labelled plates w
50. r until all four feet rest flat on the surface Turning the screw clockwise to lift the dispenser and anti clockwise to lower it Tolift the dispenser unit Turn clockwise to lift the dispenser unit and vice 99 Performance test PerkinElmer 2030 Performance Test Equipment needed 1420 442 Testplate 1420 442 Test procedure Load the service program and run the service program performance test Print the results Performance Test Ea Wale Load the test plate to the instrument FS Pertormance gt AN DOK Cancel Measure Wallac 1420 Service Program Yersion 2 00 25 Al ES File Edit Search Setup Debugger Host Window Help MEASUREMENTS Protocol start Performance PlateDim Abort Results EslideEject Reset amp lt lt c victor2ktestdatakglptest sum Restore Move Size 1999 09 13 Minimize 12 24 02 Maximize DRMANCE TEST RESULTS Version 2 00 25 25 9 Hes lH 00 01 26 56 Save 2 Save As Print DD gt FITC Dark countz 132 Instrument background 183 Sample countz 1668545 FLUOROMETER BELOW RESULTS Label Dark countz Instrument background Sample countz Signature Copyright 1994 Wallac OY 137971999 12 24 17 Check that the results are within the limits shown on the next page Note that not all instruments have all the options and the different models have different limits 100 PERKINELMER 2030 PERFORMANCE TEST R
51. say buffer Bl 34 Functional description 2 Crosstalk control Donor labelled reagent e g Eu in the buffer This 1s used to calculate the cross talk factor c expressed as the fraction of donor signal in the acceptor measuring window 3 High reference Wells containing all the reagents required to form maximal energy transfer signal The donor intensity Dmax 1s used to calculate the normalized ratio giving numeric values similar to the unquenched energy transfer intensity Normalization measurements The configuration plate wells can be run separately or as part of the assay Each well 1s measured with both donor D and acceptor A filters using identical time windows The following values are needed for calculations Bl The measurement of the buffer wells 1 1 e the plate background in the acceptor window e g at 665 nm Agonor BD Daonor this crosstalk factor is obtained from the crosstalk control wells 2 The contribution of the blank corrected donor signal in the acceptor window Agonor Bl e g with 665 nm filter using a time window from 50 to 150 us is calculated This is divided by the donor signal in the donor window Dionor e g with a 613 nm filter in the identical time window 50 150 us A typical crosstalk for the PerkinElmer chelate W1024 1s 0 0025 0 25 Dmax The donor signal from high reference wells 3 This value 1s used for normalization Sample measurements All samples are measure
52. sername and password Click Next this part 1s for setting up the communication between the PC and PerkinElmer 2030 60 Software installation PerkinElmer instrument flash update wizard Check that the instrument i properly connected to the PerkinElmer instrument interface card of your computer Switch the power of the instrument on m Press Next to proceed to Flash memory software update press Cancel to abort flash update Cancel If all connections are OK and instrument 15 switched on click Next If the instrument 1s not connected you will get the following dialogue PerkinElmer instrument flash update wizard Instrument was not detected correctly Check that interrupt level settings are coment and try again IF this problem persists run service program to check instrument Press Back to retum to settings dialog or press Cancel to undo changes and abort flash update Cancel In this case the instrument was not switched so connection could not be established Check connections and power and click the Back button when everything is OK Click the Next button again If a connection can be established the flash ROMs are updated F BIOS ROMs of the instrument so that they correspond to the program version of the instrument 87 Software installation t PerkinElmer instrument flash update wizard Programming status 5 ending new programs to the instrument Progress T
53. st height position of the arm When the height is tighten the three screws 92 Liquid dispenser installation 3 Electrical connections setup Slide the dispenser unit over the arm 1 J Connect the two connectors Opto and Motor for the robotic arm to the main instrument Note Be careful to get the connections the right way round Y gm Ss CE j em c E ie a d Connect the RS cable and the power cable between PerkinElmer 2030 and the dispenser unit Connect the dispenser unit to the mains voltage and switch the power on for both PerkinElmer 2030 and the dispenser unit 93 Liquid dispenser installation A 11111 TE Mains link from dispenser f TAULE C Re ur For arcnet cable Load the PerkinElmer 2030 Service Program initialize and then select the dispenser unit Check first the existing options System Options Show and then SetOptions add Dispenser to the existing options Calibrate the arm see calibration instructions 4 Installation of the pump unit Open the lid cover and remove the tube and pump protection cover see the figure below Lid cover Pump protection cover Remove the pump cover and screws 2x before installing the pump unit 94 Liquid dispenser installation Remove screws 2 zw T li p Pump cover lt F 4 LI Pump unit IN 4 s Ye P To install the pump unit simply slide th
54. tering into the optics at the measuring position The conveyor surface should be cleaned at least once a week using a soft cloth or tissue paper soaked in a mild detergent solution or alcohol Instruments having TR fluorometry mode In case of spillage of a sample containing any signal generation component clean the area where the spillage occurred in a similar way to that described above except that you first use Enhancement Solution to remove e g the europium then a mild detergent or alcohol and finally distilled water Let the area dry before starting to use the instrument Dispenser maintenance In the Tools menu there 15 an item called Dispenser Maintenance There 15 also an icon with the same function Clicking this menu command or icon invokes the Dispenser Maintenance Setup dialogue to perform common dispenser maintenance operations This 1s enabled only when working with a system that has the dispenser installed The current state of the instrument server 15 shown at the top of dialogue Y Dispense Maintenance Instrument status Idle Select pump s and operation 300 pl SOO pl 500 pl 500 pl a Hi Ce The setup dialogue presents you with graphical representation of number of pumps 1 4 available when performing an operation You can select the pump s that are going to perform the operation by checking boxes under the respective pump pictures The operation to be 43 Routine maintenance per
55. the flash update PerkinElmer 2030 Enhanced Security Setup Administrator account Get the username and Password for PerkinElmer 2030 administrator Password With this combination 20204dmin can lag in to PerkinElmer 2030 Manager and create Press Next to complete the Enhanced Security Setup lt Back Cancel Give the username and password for the Administrator in the Enhanced security mode Click Next 85 Software installation 22 PerkinElmer 2030 Enhanced Security Setup Enhanced Security Setup has been successfully completed PerkinElmer 030 operates now under the enhanced security mode Enhanced Security made provides you user control audit trail and data security Features Press Close to quit the Enhanced Security Setup Click Close In demo mode the installation will jump to the last screen of the installation process Installshield wizard complete In normal mode when the instrument is connected the installation will continue with the flash update i57 PerkinElmer instrument flash update wizard Welcome to PerkinElmer Flash update Wizard cdt p 60 1 Flash update wizard will guide you through the instrument Flash memory software update 1 Note If software with Enhanced Security 1s uninstalled and then reinstalled it will not ask for a new Username and Password It 15 therefore important that you keep the Admin U
56. the software for US62 0 SACNET ADAPTER Click Finish to close the wizard Complete the wizard by clicking Finish The installation of the USB22 ARCNET adapter is now ready To check that the driver is working properly right click My Computer and select Properties System Properties System Restore Automatic Updates Remote General Computer Hardware i Advanced Device Manager The Device Manager lists all the hardware devices installed on your computer Use the Device Manager to change the properties of any device Device Manager Drivers Driver Signing lets you make sure that installed drivers are compatible with Windows Windows Update lets vou set up how Windows connects to Windows Update for drivers Hardware Profiles Hardware profiles provide a way for you to set up and store different hardware configurations Hardware Profiles On the Hardware tab of System Properties click Device Manager 78 Software installation The USB ARCNET adapter should be visible under the Universal Serial Bus Controllers E Device File a X Action View Help E amp m 4 Computer Se Disk drives 23 Display adapters DVD CD ROM drives Floppy disk controllers q Floppy disk drives Human Interface Devices ATA ATAPI controllers Keyboards 5 Mice and other pointing devices Mo
57. tine maintenance This 1s maintenance intended to be performed by the user and 15 described in a separate chapter of this instrument manual Any other maintenance than what 1s described there should be performed by a qualified service person 39 Information about user instructions and warnings Warnings The following warnings are found in the User manual Note Errors in protocol selection or plate layout definition will lead to incorrect results See page 29 Starting Operation with Start Wizard CAUTION do not put your fingers into the sample loading area See pages 35 and 37 Stacker operation Note make sure you do not operate this mechanism when the stacker is loaded in PerkinElmer 2030 because you may jam the conveyor See page 36 Stacker operation Note changes to protocols should only be made by authorized persons See page 47 Protocol editor Note These operations can affect the whole system and should only be undertaken by a person qualified to do them See page 73 Tools menu In the Instrument manual on pages 92 and 95 users are reminded to make sure they save the Admin username and Password that are used in the case of Enhanced Security mode installations 40 3 Routine maintenance Cleaning the instrument Dispenser maintenance Compacting the database Changing the CW Lamp 41 Routine maintenance Routine Maintenance Cleaning the instrument The conveyor surface should be kept clean to avoid dust and dirt en
58. ting system is up and running Please wait for a while When you start up the computer the ARCNET card installed the following instructions will guide you through the installation procedure 66 Software installation Found New Hardware Wizard Welcome to the Found New Hardware Wizard This wizard helps you install software for PerkinElmer SACNET adapter model Wallac ROG If your hardware came with an installation CD 182 floppy disk insert it now What do pon want the wizard to Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue Cancel First insert the PerkinElmer 2030 CD then select Install from a list or specific location Advanced Click Next Found New Hardware Wizard Please choose vour search and installation options at Y e Search for the best driver in these locations Use the check boxes below to limit ar expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy Include this location in the search Don t search will choose the driver to install Choose this option to select the device driver from list Windows does not guarantee that the driver vou choose will be the best match for your hardware The option Search for the best driver in these locat
59. tional description UV absorbance For PerkinElmer 2030 0050 Flash excitation Block filter slide This functions as in normal PerkinElmer 2030 31 Functional description Time resolved fluorometry For PerkinElmer 2030 0050 Aperture slide Emission filter slide Polarization emission filter slide excitation Mirror filter slide block This functions as in normal PerkinElmer 2030 The same configuration 15 used for both DELFIA and LANCE technologies Functional description Luminometry For PerkinElmer 2030 0050 Apertura slide PS as E Emission filter slide MPP PPP BHEEEEEEBEEEEEENNER Polarization emission filter slide Mirror block This functions as normal PerkinElmer 2030 33 Functional description Appendix Calculation method for LANCE TR FRET normalization Introduction Before LANCE homogenous time resolved fluorometry measurements can be made the instrument must be normalized for the protocol to be used This procedure corrects for the following effects in LANCE measurements background from the plate crosstalk of the donor signal into the acceptor window quenching The final result 15 normalized to the maximum donor intensity This procedure 15 handled by a normalization wizard The measurements and calculations used to obtain the final results are described in this appendix Note 1f the normalization 1s only required for a particular plate then normalizati
60. tticalcOnnecllonsse ups Ein 93 Asta Ma HOR OL Ne LEID eier ce 94 Dispenser cabra ON A5 e tb A A nr nn 97 Equipment need ed E nee en 97 Horizontal GnGle Of th dispenser ai een 97 Measurement position of the dispenser 97 DISPENS rE anstellen 98 Pi A T rasen 99 PerkinElmer 2030 Performance Test sise 100 I prie DES C GIC res ee 100 Test procedure ri iii 100 Saving EEPROM parameters to hard disk 102 FirSE time Stat UD ana 103 An PR 107 Contents Trademarks DELFIA is a registered trademark and Wallac and LANCE are trademarks of PerkinElmer Inc Windows is a registered trademark of Microsoft Corporation in the U S and other countries Pentium is a registered trademark of Intel Corporation 1 Functional description Introduction Models and options Features of all models except PerkinElmer 2030 0050 Features of PerkinElmer 2030 0050 Functional description PerkinElmer 2030 multilabel reader Introduction PerkinElmer 2030 multilabel reader 1s a complete platform for quantitative detection of light emitting or light absorbing markers Depending on the model of the instrument you have 1t 18 suitable for flash or glow luminometry fluorometry high sensitivity time resolved fluorometry DELFIA UV absorbance photometry homogeneous time resolved fluorescence assays LANCE option and fluorescence polarization It 15 a compact bench top unit with features suc
61. unlight should not be able to reach the instrument Electric power Three electrical outlets each with a protective earth should be available with 1f possible a separate power line for the instrument itself having an isolation switch and a fuse box If excessive fluctuations in the mains voltage are anticipated a mains stabilizer may be necessary 61 Instrument installation Unpacking 1 Cut the belts and remove screws 2 Lift the lid off 3 Lift out the accessories and check them according to the packing list 4 Lift the instrument up and move 1t to 1ts place of operation 5 For the stacker model remove the transport protection piece see fig on next page 6 Check for possible damage 62 Instrument installation Removing the Transport Protection Piece For preventing the stacker from moving and getting damaged during the transport there 1s a protection piece assembled Remove this by opening the screws and slide the piece out from the stacker Check the mains voltage setting The fuse holder of the mains voltage selector 1s covered by the following sticker SITIO Check the mains voltage setting The fuse holder of the mains voltage selector is set to 220 240V Check that it is according to the voltage of the mains the instrument will be connected to If not set the fuse holder to the correct position FO E DA FS m Note Make sure the correct size of fus

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