HSC-40 User`s Manual
Contents
1. esses 10 3 6 Draining the Extraction Fluid nennen nnns 11 3 7 Installing the CO 11 3 8 Removing the CO 12 3 9 Cleaning the Extraction Fluid Reservoir 1096 Bleach with Sodium Thiosulfate Deactivation p 12 3 10 Cleaning the Extraction Fluid Reservoir 3 Hydrogen 14 3 11 Calibration of the Optical 5 15 3 12 Calibration of the Pressure Transducers 16 3 13 Concentration Cell Pressure Check esee enne nennen 17 CHAPTER 4 HSC 40 Operation necant eee ec P DER TARDE 18 4 1 LED Control Panel e t re e e P Re M e D tan Pe erg 18 4 2 MaIFUSGEB OT o ronis e dt eed deste e e e eyes 19 4 3 Setting the Extraction Fluid Volume and 2 20 4 4 Concentrating a Liquid Sample ssseseeseeeeeee eee enenatis 24 4 5 Additional Sample nnns nnne nnns 27 Rev 3 January 3 2010 Www innovaprep com 1 4 6 Concentration Cell Decontamination 1 27 4 7 Autoclaving of Concentration Cells cccccssccccecessssesssceceeeceseeseaeaeceeeceseeseaeaeee2. 8 3E 8 R Recommended n p 2 2 gt Sv E L Limited Exposure gt d S S gt 2s jet 2 2 2 NR Not Recommended o 22 8 1822 E E gt U Unknown _ 5 gt s 2 3 Flas Sle Pyridine NR NR NR R L L R NR Silicone oil R R R R R R R R UJ R 0 1 N Sodium hydroxide R L R R R R R JL A R R 5 Sodium hydroxide R NR L R R R R 25 Sodium hydroxide LER NR L R R R R L NR R 5096 Sodium hydroxide NR Pire NR Conc Sodium Hydroxide R R R L L NR R Sodium hypochlorite R L R NR U R 5 Sulfuric acid R R R L 25 Sulfuric acid L R R R R 6 Sulfuric acid R R R Conc Sulfuric Acid NR NR NR NR R Tetrahydrofuran NR NR R NR R R Toluene NR IR RJL R R R 25 Trichloroacetic acid INR NR R R R L NR NR R Trichlorobenzene U NR R NR R R U U Trichloroethane INR L R R Trichloroethylene R INR L L I R R Triethylamine Turpentine Urea R R R LJ R R 6N Urea LU NR R NR R Water BR R R RIR RJ R Xylene NR NR R R L NR R Rev 3 January 3 2010 www innovaprep com 32 CHAPTER 5 Maintenance 5 1 Maintenance Schedule A regular maintenance schedule should be strictly followed in order to kee
3. gt Test extract 14 Adjust the Foam timer until the desired extraction volume is achieved using the following sequence of menu options Main menu Run Standard run Settings Extract settings gt Foam timer 4 4 Concentrating a Liquid Sample 1 Place a feed sample into the Feed reservoir on the front panel of the HSC 40 2 Place a sample concentrate tube under the retentate tube on the bottom of the concentration cell Rev 3 January 3 2010 www innovaprep com 24 3 Perform Standard Run using the following sequence of menu options Main menu Run Standard run Screen shots of the appropriate menu selections are shown below in Figure 4 10 through Figure 4 12 Figure 4 10 Main Menu Rev 3 January 3 2010 Www innovaprep com 25 Figure 4 12 Standard Run 4 Press Start on the Standard Run menu screen 5 Sample will be automatically processed and the concentrate will be dispensed into the sample concentrate tube Rev 3 January 3 2010 www innovaprep com 26 4 5 Additional Sample Extractions Additional extractions of the concentration cell can be performed by pressing Extract Again 4 6 Concentration Cell Decontamination Decontamination of the concentration cell may be necessary in some instances Decontamination can be performed with any of the methods described below Perform one or more additional extractions of the concentration cell to remove remaining material from
4. viruses DNA other particles of biological origin including pathogens for subsequent analysis by microbiological methods or with microbiological detection devices 1 3 How the InnovaPrep HSC 40 Operates The InnovaPrep system uses a novel wet foam elution method to recover organisms that have been captured onto hollow fiber membrane filters into user settable volumes as small as 50 microliters The wet foam is produced by releasing a surfactant solution held under a carbon dioxide head pressure to an ambient pressure with a timed valve Appropriate surfactant solutions include 0 025 to 0 25 Tween 20 Triton X 100 or other surfactants or proteins PBS Tris or other buffers may be added to control the solution pH InnovaPrep can provide additional information and recommendations for extraction solution selection 1 4 InnovaPrep History The technology was developed by a team of veteran aerosol research scientists in response to the recognized need for a macro to micro interface between the large liquid sample volumes collected by many bioaerosol samplers and advanced detectors which require ultra low volumes InnovaPrep was founded in 2008 to commercialize this new concentration and sample preparation technology developed by AlburtyLab Inc and Page Applied Research LLC Rev 3 January 3 2010 www innovaprep com 2 CHAPTER 2 Description of the InnovaPrep HSC 40 The following chapter describes the components of the InnovaPrep H
5. Recommended L Limited Exposure NR Not Recommended U Unknown 5 Acetic acid Mixed Cellulose Ester ME Fee el lc eG cl cet Ge Gl pene RC Polyethersulfone PES Cellulose Ester difluoride PVDF Polysulfone PS Polyvinylidene 25 Acetic acid R Acetic acid glacial R Acetone R R N Acetonitrile R NR N Dilute Ammonium hydroxide Conc Ammonium hydroxide CARS RIC AIG A 20 Fluorocarbon Sample Fluidics 2 2 R R R R PR RI RIL LE DR NR R R RS R acetate R L Amyl alcohol Aniline R R Benzene R Benzyl alcohol R Boric acid Brine Bromoform R U Butyl acetate R R Butyl alcohol L Butyl cellosolve L R U Butylaldehyde U R U Carbon tetrachloride R R NR Cellosolve L Chloroacetic acid R R NR Chloroform R TR R Chromic acid L NR NR NR L R NR U N Rev 3 January 3 2010 www innovaprep com R Recommended L Limited Exposure NR Not Recommended U Unknown Cresol Cyclohexane Cyclohexanone Diacetone alcohol Dimethyl formamide Dimethylsulfoxide 1 4 Dioxane Ethers Ethyl acetate Ethyl Alcohol 15 Ethyl alcohol 95 Ethyl alcohol Ethylene dichloride Ethylene glycol Ethylene oxide 2 Formaldehyde 30
6. fluid systems must be clean and completely dry Any residual liquid or bubbles in the tubing could interfere with accurate calibration of the optical sensors The following procedure should be used to calibrate the optical sensors 1 Press maintenance on the home screen 2 Press system settings 3 Press Sensor Cal 4 Press Enter Rev 3 January 3 2010 Www innovaprep com 15 Figure 3 10 Sensor Calibration Screen After the calibration is complete a pass fail page will appear with four light bulbs which corresponds to the four optical sensors An illuminated light bulb indicates an optical sensor did not calibrate correctly In most situations sensor 3 will fail calibration because it is not plugged into the external port If you are not using the external fluid sensor then disregard the sensor 3 fail state and continue 3 12 Calibration of the Pressure Transducers In order to calibrate the pressure transducers the following items are required Pressure gauge with a minimum 10 psi reading capability 60 ml syringe Three pieces of tubing that fit 4 28 fittings 4 in 2 in and 10 in pieces Barbed ubing Assemble the above items as indicated in shown below in Figure 3 2 Rev 3 January 3 2010 Www innovaprep com 16 here Figure 3 2 Calibration of the Pressure Transducers To calibrate the pressure transducers use the following procedure Press maintenance on the home screen Pre
7. reservoir must be filled with an appropriate reagent for sample extraction This fluid must be provided by the user Instructions for loading the extraction fluid is included in Section 3 5 A personal computer is required for uploading new protocols to the HSC 40 PLC Requirements for the personal computer that will be used to upload protocols to the PLC are summarized below Rev 3 January 3 2010 www innovaprep com 9 3 3 Installing Concentration Cell To install a concentration cell refer to Figure 3 1 and the following procedure 1 2 3 4 Uninstalling a Concentration Cell To uninstall a concentration cell refer to Figure 3 1 and the following procedure 1 2 3 Note appropriate storage fluid and capped prior to storing Contact InnovaPrep regarding questions about specific membrane types 816 619 3375 3 5 Filling the Extraction Fluid Reservoir 1 ae Hold the concentration cell with the arrow on the label pointing up Attach the upper end of the concentration cell to connector A Pass the tubing on the lower end of the concentration cell through the fluid sensor and retentate valve pinch point Attach the connector B to the concentration cell Attach the connector C to the concentration cell Detach connectors A and B and place caps or plugs into the ports Detach connectors C and place caps or plugs into the ports Detach the tubing from the bottom port of the concentration cell and r
8. Formaldehyde 25 Formic acid 50 Formic Acid Freon Gasoline Glycerine Glycerol Hexane Hexanol 5 Hydrochloric acid 25 Hydrochloric acid 37 Hydrochloric acid 25 Hydrofluoric acid 30 Hydrogen peroxide Iodine solutions Isobutyl alcohol Isopropanol Isopropyl acetate Isopropyl alcohol amp a wm gl 5 5 es m CEN un Too 0 m o L aA prd A Sige as 25 gt d 8ls53 le3 5 3 amp 3 o3 2 T az 22 e 92 cU gt 6 5 D oo gt 5 O i52 8 gt n e gt 5 a NR U NR L R L R R R R U NR NR R NR R L R R R R R L E E E E L 22 2 2 212 2121 12 FERE RI LU amp R L NR U Pe Rk PNR U L R R R RT NR L R E R Es R R NR AAA AAA AA E 9 C 1179 79 79 79 Fluorocarbon Rev 3 January 3 2010 www innovaprep com w R Recommended L Limited Exposure NR Not Recommended U Unknown Isopropyl ether Jet Fuel 640A Kerosene Lactic acid Methyl acetate Methyl alcohol 98 Methyl alcohol Methyl cellosolve Methyl chloride Methyl ethyl ketone Methyl formate Methylene chloride N meth
9. InnovaPrep 5 40 Hydrosol Concentrator User s Manual JL INNOVAPREP SS The Macro to Micro Interface Table of Contents CHAPTER 1 Product OVervi W 1 LT Product Description oe oen edente tette to ae eee 1 1 2 gt E T e Sea iv TU ae 1 1 3 How the InnovaPrep HSC 40 0 000 2 1 4 HIStory rre ee esee Poe ete 2 2 Description of the InnovaPrep 5 40 1 2 1 Front Panel go e ite tt e RR e eee o ERE ERR NR Puede e 1 2 2 Concentration Cell Panel ater rere tere rette ce d et EP 4 2 3 tte trt RE etie eae te ARR erae 6 2 4 Back Paneli H 7 2 5 internal Componerits creer ine tec En rti a 8 CHAPTER 3 Installati n ierit e e REI ARA ue ede Rena 9 3 1 Materials Provided eate t n 9 3 2 Materials Required But Not 2 9 3 3 Installing a Concentration 4 00 eene enne nnne entia nnns 10 3 4 Uninstalling a Concentration Cell esee nennen nnne nnns 10 3 5 Filling the Extraction Fluid Reservoir
10. SC 40 2 1 Front Panel The following sections describe the main components of the front panel of the InnovaPrep HSC 40 as is shown in Figure 2 1 5 40 Figure 2 1 InnovaPrep Model HSC 40 Front Panel Rev 3 January 3 2010 Www innovaprep com 2 1 1 LCD Control Panel Functions of the HSC 40 are managed through a user interface LCD control panel located in the upper left hand corner of the front panel Detailed instructions on the operation of the LCD control panel are provided in Section 4 1 2 1 2 Power Switch Power to the HSC 40 is controlled by the Power on off switch located to the right of the LCD control panel 2 1 3 Reservoirs Two fluid reservoirs are located directly below the LCD control panel The left hand reservoir serves as the sample Feed reservoir and the right hand reservoir serves as the Permeate reservoir Samples to be processed may be introduced to the HSC 40 through the feed reservoir As the sample is processed through the concentration cell the permeate is collected in the permeate reservoir Each reservoir is detachable for easy replacement and or cleaning 2 1 4 Pumps Two liquid peristaltic pumps are located directly to the right of the fluid reservoirs and below the power switch The upper is the feed pump and the lower serves as the permeate pump Each pump is controlled to maintain feed and permeate pressures set through the LCD control panel 2 1 5 Control Valves Four r
11. Switching to vent with a cylinder fully engaged will vent the entire cylinder Follow the instructions for removing the cylinder prior to turning the valve to this position In this position the supply lines are connected and ready for operation In this position the supply lines are valved off from the system including the extraction fluid reservoir In this position the CO head pressure is vented from the extraction fluid reservoir to the side port labeled Vent located above Extraction fluid reservoir label 2 1 6 Extraction Fluid Level Indicator The volume of fluid remaining in the extraction fluid reservoir is indicated by the Extraction fluid sight glass located to the right of the control knobs directly below the pumps 2 1 7 Pressure Gauges Two pressure gauges located below the control knobs display the pressure in the CO supply pressure cylinder and in the Extraction fluid pressure reservoir Directly below Rev 3 January 3 2010 Www innovaprep com 3 the extraction fluid pressure gauge is a PSI adjustment screw Using the Allen wrench located below the supply pressure gauge the gas pressure in the extraction fluid reservoir can be easily adjusted NOTE When the pressure is increased the operator should wait 20 minutes prior to processing a sample This allows for the to come into equilibrium with the extraction fluid 2 2 Concentration Cell Panel The following sections
12. ack Panel An access panel is located on the back side of the HSC 40 Internal components must be service by a certified InnovaPrep technician Located on the upper right hand corner of the back panel is the operating system port This port is used to download software updates to the system Please contact InnovaPrep 816 619 3374 for assistance Rev 3 January 3 2010 Www innovaprep com 7 Figure 2 4 InnovaPrep Model HSC 40 Back Panel 2 5 Internal Components Internal components must be service by a certified InnovaPrep technician Please contact InnovaPrep 816 619 3374 for assistance Rev 3 January 3 2010 Www innovaprep com 8 CHAPTER 3 Installation Included in this chapter are the general instructions for the setup of the InnovaPrep 5 40 Inspect each component when unpacking If damage is observed do not use the instrument and contact your shipping representative immediately Remove any protective packaging that may be present around the HSC 40 instrument Examine the packaging material to be sure that materials that have been provided see the list below are removed 3 1 Materials Provided Component Qty InnovaPrep HSC 40 Power Cord Programming Cable Concentration Cells 9 oz CO Cylinder Female Luer Lock Permeate Connectors NF N Male Luer Lock Permeate Connectors Decon Jumper Tube 1 3 2 Materials Required But Not Provided In order to use the system the extraction fluid
13. ained in Section 3 6 Draining the Extraction Fluid Reservoir to remove any remain extraction fluid 2 Clean with 3 hydrogen peroxide Refer to Figure 3 1 for the location of the lettered points contained in these procedures a Runa flexible line from the Drain and Vent ports to a waste container this should have already been performed when draining the extraction fluid reservoir Slowly set the CO valve to Reservoir vent c Wait for the venting gas to stop and then set the Extraction fluid valve to Fill d Remove line from the concentration cell and attach a section of tubing from line B to a waste container e Attach a section of tubing to the Fill port and attach to the outlet of a small external pump a syringe may also be used f Place the inlet tube of the external pump into 500 ml vial filled with 300 ml of 396 hydrogen peroxide a syringe may also be used g HSC 40 on and navigate to the settings menu and select Run Standard Run Settings Foam Consistency but do not hit start Rev 3 January 3 2010 Www innovaprep com 14 1 Run external pump use the syringe until fluid 3 hydrogen peroxide flows out of the Drain and Vent ports Press the start button on the HSC 40 control panel and continue filling until fluid starts to drip out of line B after about 10 seconds hit the stop button on the control panel then stop the external pump Let the 3 hydrogen peroxide so
14. aneously and carefully open retentate valve with the end of a standard screw driver and pinch the permeate pump peristaltic tubing between your thumb and index finger Allow 5 ml of the fluid to drain out into the catch cup release tube and valve allow run to finish When run is complete refill reservoir as noted in step 1 with filtered DI water Set feed knob to drain reservoir and pull all of the water out with syringe Repeat this step Refill reservoir as in step 1 with filtered DI water Start standard run after 1 min open retentate and pinch permeate line Allow 20 mils to flow through fiber bore close retentate and release perm line allow 20 Rev 3 January 3 2010 Www innovaprep com 27 mills to flow out perm repeat until only 5 mills remain then allow run to complete itself Repeat this step 15 Fill feed res with 15 ml of storage fluid 16 Run standard run after 30 sec open retentate allow storage solution to drip out for 5 seconds the turn off unit 4 7 Autoclaving of Concentration Cells The following autoclave method is recommended for use with hollow fiber modules 1 a Remove and discard all pouches as these are not autoclavable wet membrane optional Loosen all sanitary connections Luer fittings clamps and flange nuts to avoid damaging the module during the autoclave cycle Wrap all open ports of the connecting tubing with autoclave paper Pre warm the module and use pre vacuum steps if
15. ation of the lettered points contained in these procedures a j k Run a flexible line from the Drain and Vent ports to a waste container this should have already been performed when draining the extraction fluid reservoir Slowly set the valve to Reservoir vent Wait for the venting gas to stop and then set the Extraction fluid valve to Fill Remove line B from the concentration cell and attach a section of tubing from line B to a waste container Attach a section of tubing to the Fill port and attach to the outlet of a small external pump a syringe may also be used Place the inlet tube of the external pump into a 500 ml vial filled with 300 ml of 10 bleach a syringe may also be used Turn the HSC 40 on and navigate to the settings menu and select Run Standard Run Settings Foam Consistency but do not hit start Run external pump or use the syringe until fluid 10 bleach flows out of the Drain and Vent ports Press the start button on the HSC 40 control panel and continue filling until fluid starts to drip out of line B after about 10 seconds hit the stop button on the control panel then stop the external pump Let the 10 bleach solution remain in the extraction fluid reservoir for 10 minutes Follow procedures contained in Section 3 6 Draining the Extraction Fluid Reservoir to remove the 10 bleach 3 Deactivate the 10 bleach with 1 sodium thiosulfate a b 6 g h Sl
16. describe the main components of the concentration cell panel of the InnovaPrep HSC 40 located behind the Plexiglas door as is shown in Figure 2 2 Figure 2 2 InnovaPrep Model HSC 40 Concentration Cell Panel Rev 3 January 3 2010 Www innovaprep com 2 2 1 Flow Rate Needle Valves Two adjustable flow rate needle valves are located at the top of the concentration cell panel The left needle valve adjusts the external fluid flow rate and is identified as Ext fluid flowrate The right needle valve adjusts the CO flush flowrate 2 2 2 Height Adjustment Carriage The height adjustment carriage is located on the left side of the concentration cell panel This easy to use adjustment allows for the use of concentration cell of varying lengths To adjust the height depress the black spring loaded knob and move the carriage as needed 2 2 3 Retentate Valve The retentate valve is attached to the height adjustment carriage and attaches to the bottom tubing of the cell This valve controls the flow of liquid from the concentration cell 2 2 4 Concentrate tray The concentrate tray is located just below the height adjustment carriage and is also height adjustable This stainless steel tray provides sa location for various sample containers and tube holders to be placed for collection of the concentrate sample from the concentration cell 2 2 5 Protective Door A clear protective door covers the entire concentration cell panel During the conce
17. ed above the permeate valve In this position fluid can be drained from the onboard permeate reservoir manually using a syringe connected to the side port labeled Permeate reservoir drain NOTE The valve must not be in this position when the permeate pump is running The Extraction fluid valve has four positions as follows Fill Run Drain Off In this position the extraction fluid reservoir is connected to the side port labeled Fill located above the Extraction fluid reservoir label The reservoir can be filled through this port Note Vent reservoir pressure completely prior to switching valve to this position In this position the extraction fluid reservoir is connected to the wet foam valve and ready for extraction In this position the fluid can be drained from the extraction fluid reservoir to the side port labeled Drain located above Extraction fluid reservoir label Note Vent the extraction fluid reservoir until an extraction fluid pressure of less than 50 psi is reached Also attach a length of tubing with the outlet in a waste container prior to switching valve to this position In this position the extraction fluid reservoir is disconnected from the fill drain and run fluidics valve has four positions as follows vent Run Off Reservoir vent In this position the supply lines are connected to the side port labeled Vent located above Extraction fluid reservoir label Note
18. ed faced control valves are located below the fluid reservoirs These control the flow of the sample feed permeate extraction fluid and The desired position should be selected by slowly rotating the valve clock wise Built in positioning detents allow the valves to snap into place ensuring correct positioning of the valves The position settings for each of the control valves are described below The Feed valve has three positions as follows noted side ports are located on the left hand side of the instrument From side port In this position the feed pump will draw fluid from the side port labeled Feed in to the concentration cell From reservoir this position the feed pump will draw fluid from the onboard Feed reservoir located directly above the feed valve to the concentration cell Drain reservoir In this position fluid can be drained from the onboard feed reservoir manually using a syringe connected to the side port labeled Feed reservoir drain NOTE The valve must not be in this position when the feed pump is running The Permeate valve has three positions as follows To side port In this position the permeate pump will direct fluid from the concentration cell permeate to the side port labeled Permeate out Rev 3 January 3 2010 Www innovaprep com 2 reservoir Drain reservoir In this position the permeate pump will direct fluid from the concentration cell permeate to the onboard Permeate reservoir locat
19. eplace with a cap or plug The concentration cell must be filled with an If the extraction fluid pressure will remain the same it is not necessary to adjust the Extraction fluid Figure 3 1 Concentration pressure regulator prior to filling however if a Cell Installation lower pressure is desired begin by inserting the hex key found under the CO supply pressure gauge into the opening for the extraction fluid pressure regulator and turning several counterclockwise turns to reduce the pressure to less than the desired pressure if resistance is met stop turning Slowly set the valve to Reservoir vent Wait for the venting gas to stop and then set the Extraction fluid valve to Fill Load a syringe with extraction fluid and attach it to the Fill port on the left side of the HSC 40 Rev 3 January 3 2010 www innovaprep com 10 the extraction fluid reservoir with no more than 125 mL of extraction fluid This will allow sufficient space at the top of the reservoir for the NOTE the Extraction fluid level gauge will not register the correct volume of extraction fluid until the system has been pressurized Use the syringe to track the volume of extraction fluid loaded Switch the Extraction fluid valve to Run Switch the CO valve to Off Using the hex key found under the supply pressure gauge slowly turn the Extraction fluid pressure regulator until the desired pressure is reached NOTE It may take several
20. er 4 2009 Rev 3 written for software version 1 0 January 3 2010 Rev 4 written for software version 1 0 Rev 3 January 3 2010 www innovaprep com 35
21. eseeseeseaaeas 28 4 8 Concentration Cell 5 enne 28 4 9 Cell Selection iui 29 CHAPTERS Maintenatice oe tree a tenete do Ye Ree vnu ave RU ER Ye de Eu 33 5 1 Maintenance Schedule 555 5 eet 33 5 2 Tubing Replacement i ER re En URN 33 CHAPTER 6 2 34 CHAPTER 7 R vision 35 Rev 3 January 3 2010 www innovaprep com ii CHAPTER 1 Product Overview This chapter contains a product description of the InnovaPrep Model HSC 40 hydrosol concentrator and a brief description of instrument operation 1 1 Product Description The Model HSC 40 hydrosol concentrator shown n Figure 1 1 quickly and efficiently concentrates waterborne bacteria viruses DNA toxins protozoa or other particles of interest from large liquid sample volumes into liquid volumes as small as 50 microliters The HSC 40 system uses a novel elution method to recover organisms that have been captured onto hollow fiber membrane filters membranes into very small volumes InnovaPrep 5 40 7 Figure 1 1 InnovaPrep Model HSC 40 Hydrosol Concentrator 1 2 Applications The InnovaPrep technology has application anywhere that analysis of low concentrations of particles is needed The primary area of application is for preparing and concentrating Rev 3 January 3 2010 www innovaprep com 1 bacteria
22. lution remain in the extraction fluid reservoir for 30 minutes Follow the procedures contained in Section 3 6 Draining the Extraction Fluid Reservoir to remove the 3 hydrogen peroxide 3 Rinse with sterile filtered laboratory water a b Slowly set the valve to Reservoir vent Wait for the venting gas to stop and then set the Extraction fluid valve to Fill Place the inlet tube of the external pump into a 500 ml vial filled with 300 ml of sterile filtered laboratory water a syringe may also be used Turn the HSC 40 on and navigate to the settings menu and select Run Standard Run Settings Foam Consistency but do not hit start Run external pump or use the syringe until fluid sterile filtered laboratory water flows out of the Drain and Vent ports Press the start button on the HSC 40 control panel and continue filling until fluid starts to drip out of line B after about 10 seconds hit the stop button on the control panel then stop the external pump Follow the procedures contained in Section 3 6 Draining the Extraction Fluid Reservoir to remove the sterile filtered laboratory water Hydrogen peroxide test strips must then be used to ensure that the hydrogen peroxide solution has been completely removed Repeat the rinse step until the hydrogen peroxide concentration is below the acceptable level 3 11 Calibration of the Optical Sensors Warning Before calibrating the optical sensors the InnovaPrep HSC 40
23. ntration cell extraction step a vacuum pump pulls air from the concentration panel section through a HEPA filter The door encloses the area around the concentration cell thus controlling aerosols that may be generated as the concentrate is dispensed The door swings outward for unobstructed access to the fiber The door should be closed while processing samples containing bioparticles 2 2 6 Aerosol Collection Tube and Shroud An aerosol collection tube and shroud are attached to the height adjustment carriage Concentrate sample tubes should be positioned on the concentrate tray so that the aerosol shroud is positioned directly above the concentration cell outlet Rev 3 January 3 2010 Www innovaprep com 5 2 3 Left Side Panel The following sections describe the main components of the side panel of the InnovaPrep HSC 40 as is shown in Figure 2 3 Figure 2 3 InnovaPrep Model HSC 40 Side Panel 2 3 1 Liquid Cylinder Port A 9 oz liquid cylinder is required for carbonating the extraction fluid The cylinder is installed in the middle of the left side panel of the InnovaPrep HSC 40 by screwing in clockwise until resistance is met Rev 3 January 3 2010 www innovaprep com 2 3 2 Power Cord Inlet An AC power cord is provided for use with the three prong AC input connector located at the bottom of the InnovaPrep HSC 40 side panel Using the power cord provided connect the AC Power Input to an approved power source For pro
24. owly set the CO valve to Reservoir vent Wait for the venting gas to stop then set the Extraction fluid valve to Fill Place the inlet tube of the external pump into a 500 ml vial filled with 300 ml of 1 sodium thiosulfate a syringe may also be used Turn the HSC 40 on and navigate to the settings menu and select Run Standard Run Settings Foam Consistency but do not hit start Run external pump or use the syringe until fluid 1 sodium thiosulfate flows out of the Drain and Vent ports Press the start button on the HSC 40 control panel and continue filling until fluid starts to drip out of line B after about 10 seconds hit the stop button on the control panel then stop the external pump Let the 1 sodium thiosulfate solution remain in the extraction fluid reservoir for 1 minute Follow the procedures contained in Section 3 6 Draining the Extraction Fluid Reservoir to remove the 1 sodium thiosulfate 4 Rinse with sterile filtered laboratory water a b Slowly set the CO valve to Reservoir vent Wait for the venting gas to stop and then set the Extraction fluid valve to Fill Rev 3 January 3 2010 Www innovaprep com 13 Place the inlet tube of the external pump into 500 ml vial filled with 300 ml of sterile filtered laboratory water a syringe may also be used d Turn the HSC 40 on and navigate to the settings menu and select Run Standard Run Settings Foam Consistency but do not hit start ex
25. p the InnovaPrep HSC 40 in optimal working condition Please adhere to the following maintenance schedule Daily Weekly Monthly Yearly 5 2 Tubing Replacement If any of the exposed tubing on the outside of the InnovaPrep HSC 40 needs to be replaced follow these steps Changing pump tubing SO Ue SI OIOV E Squeeze black side buttons on pump housing to open Remove old tubing by disconnecting the tubing from the connectors Lift old tubing out of pump slot Put o ring around new tubing Even up the ends of the tubing Drape middle of tubing over the pump slot Slightly pull tubing downward to get rid of any slack Connect tubing ends to connectors Close pump housing Rev 3 January 3 2010 Www innovaprep com 33 CHAPTER 6 Troubleshooting Problem During a standard run if the HSC 40 will not progress past the Starting Up window Check Permeate pressure If it is low it is an indication that there is a loose connection on the permeate side Tighten both thumb screws on the permeate side Please contact InnovaPrep for technical assistance and troubleshooting InnovaPrep LLC 132 E Main St Drexel MO 64742 Phone 816 619 3375 info www innovaprep com Rev 3 January 3 2010 www innovaprep com 34 CHAPTER 7 Revision History Rev 1 written for software version 1 0 July 21 2009 Rev 2 written for software version 1 0 Novemb
26. possible Slowly ramp up the temperature of the autoclave Use a ramp time of minutes or half of the hold time of autoclave whichever is LARGER Hold the autoclave at 121 C for 30 minutes do not exceed 124 C Slowly ramp down the temperature of the autoclave to room temperature Use a ramp down time of 15 minutes or half of the hold time of autoclave whichever is LARGER A one minute dry cycle is acceptable though not necessary Tighten all sanitary connections Luer fittings or any other clamps or connections if they are fitted in the module 4 8 Concentration Cell Storage Concentration cells are generally stored in 1 hydrogen peroxide or 20 ppm bleach The cells should be rinsed with sterile filtered water prior to use and tested with chlorine or hydrogen peroxide test strips to ensure complete removal Please contact InnovaPrep 816 619 3375 for assistance in selection of other storage fluids or with dry storage Rev 3 January 3 2010 www innovaprep com 28 4 9 Cell Selection Table 4 1 contains a chemical resistance chart for the various components of the HSC 40 system This chemical resistance chart is intended for use as a guide not as a guarantee of chemical compatibility Variations in temperature concentrations durations of exposure and other factors may affect the use of the product It is recommended that testing be performed under the user s specific conditions Table 4 1 Chemical Resistance Chart R
27. rol panel When the 5 40 is turned on the controller will perform a self test as shown in Figure 4 1 Figure 4 2 shows the LCD screen after the controller has passed the self test Figure 4 1 Controller Self test Running Rev 3 January 3 2010 www innovaprep com 18 Figure 4 2 Controller Self test Passed 4 2 Main Screen On the main screen are the InnovaPrep HSC 40 Logo the Run and the Maintenance options From the home screen shown in Figure 4 3 the user may select the Maintenance or Run menus with the selection buttons to the left or right of the menu item Figure 4 3 Selection of the Run menu from the Home Screen Rev 3 January 3 2010 Www innovaprep com 19 4 3 Setting the Extraction Fluid Volume and Consistency Prior to performing sample concentration runs the extraction fluid volume and consistency must be set The procedure for setting these is outlined below 1 Set the valve to Off 2 Set the extraction fluid pressure to desired level by removing the hex key from below the supply pressure gauge inserting the hex key into the regulator opening just below the Extraction fluid pressure gauge and turning it slowly until the desired pressure is attained 3 Set the valve to 4 Wait a minimum of 10 minutes for the to dissolve into the extraction fluid 5 Enter the Foam consistency menu using the following sequence of menu options Main menu R
28. s to flow the CO supply pressure gauge should show the pressure dropping If the pressure is not dropping unscrew the cylinder further until the pin valve fully disengages 5 Once the CO supply pressure reaches zero the CO cylinder can be removed 3 9 Cleaning the Extraction Fluid Reservoir 10 Bleach with Sodium Thiosulfate Deactivation Before or after long term storage or in the event that the HSC 40 fluidics become contaminated it may be necessary to clean the extraction fluid reservoir and fluidics In general it is not necessary to clean or decontaminate the extraction fluid reservoir and fluidics providing that good clean laboratory procedures are used In event that cleaning of the extraction fluid reservoir is required a cleaning procedure is provided below and an alternate procedure is provided in Section 3 10 The procedure outlined below uses a three step approach where A the reservoir is cleaned using 1096 bleach solution B the bleach is deactivated using 196 sodium thiosulfate solution and C the reservoir is rinsed using sterile filtered laboratory water It is important to follow this procedure closely to ensure that all fluidics components are cleaned 1 Drain Reservoir Follow procedures contained in Section 3 6 Draining the Extraction Fluid Reservoir to remove any remain extraction fluid Rev 3 January 3 2010 Www innovaprep com 12 2 Clean with 10 bleach Refer to Figure 3 1 for the loc
29. ss system settings Press transducer zero Follow screen prompts Disconnect A and B connectors on the concentration cell Refer to Figure 3 1 Press next Press zero reset this will reset the zero on the pressure transducers Press verify to make sure the transducers where zeroed Press pass if setting is 0 00 0 Press Fail if setting is other then 0 00 and repeat steps 7 9 1 At this point you may continue by setting the 1OPSI This step does not need to be performed every time 12 Re connect ports A and B to the cell NOTE if the cell is new it must be wetted first 13 To set the 10 PSI connect the syringe pressure gauge and attach to the right feed connector 14 Press set 10 PST 15 Press next 16 Apply 10 PSI of pressure by pressing down on the syringe plunger 17 Hold it steady at 10 PSI 18 Press next to set at 10 PSI 19 Repeat steps 13 18 for the permeate p Ip cg dy Dh de ua peces 3 13 Concentration Cell Pressure Check To pressure check the concentration cell use the following procedure Instructions not available at this time Instructions will be with the software update Rev 3 January 3 2010 Www innovaprep com 17 CHAPTER 4 HSC 40 Operation Included in this chapter are the general instructions for operation of the InnovaPrep 5 40 4 1 LCD Control Panel Control of the InnovaPrep 5 40 is performed using the LCD cont
30. tection against electrical shock hazards the HSC 40 must be directly connected to an approved power source such as a 3 wire grounded receptacle An extension cord must not be used If the power cable becomes cracked frayed broken or otherwise damaged it must be replaced immediately with the equivalent parts 2 3 3 Fuse Housing One fuse is used on the InnovaPrep HSC 40 instrument This fuse housing is located below the CO slot and contains a 3A 250V fuse To replace the fuse shut down the InnovaPrep HSC 40 disconnect the instrument from the power source and unscrew the cap 2 3 4 Multi Stage Communications Port A multi stage communications port is located to the right of the fuse housing This port is for protocols that require the use of multiple units linked together or for communication with other instruments Please contact InnovaPrep 816 619 3375 for information regarding this feature 2 3 5 Connectors There are four vertical 1 4 28 flat bottom unions located to the right of the liquid cylinder port These are labeled as Feed in Feed reservoir drain Permeate out and Permeate reservoir drain Use of these ports was described previously in Section 2 1 5 There are also three horizontal 1 4 28 flat bottom unions above the Extraction Fluid Reservoir label located in the bottom right corner of the side panel These are labeled as Fill Drain and Vent Use of these ports was described previously in Section 2 1 5 2 4 B
31. ternal pump or use the syringe until fluid sterile filtered laboratory water flows out of the Drain and Vent ports f Press the start button on HSC 40 control panel and continue filling until fluid starts to drip out of line B after about 10 seconds hit the stop button on the control panel then stop the external pump g Follow procedures contained in Section 3 6 Draining the Extraction Fluid Reservoir to remove the sterile filtered laboratory water h Chlorine test strips may be used to ensure that the bleach solution has been completely removed 3 10 Cleaning the Extraction Fluid Reservoir 3 Hydrogen Peroxide Before or after long term storage or in the event that the HSC 40 fluidics become contaminated it may be necessary to clean the extraction fluid reservoir and fluidics In general it is not necessary to clean or decontaminate the extraction fluid reservoir and fluidics providing that good clean laboratory procedures are used In event that cleaning of the extraction fluid reservoir is required a cleaning procedure is provided below and an alternate procedure is provided in Section 3 9 The procedure outlined below uses a two step approach where A the reservoir is cleaned using 3 hydrogen peroxide and B the reservoir is rinsed using sterile filtered laboratory water It is important to follow this procedure closely to ensure that all fluidics components are cleaned 1 Drain Reservoir Follow the procedures cont
32. the concentration cell Place clean feed fluid filtered laboratory grade water or surfactant solution into the feed reservoir and run as a sample Follow with additional extraction as necessary Place 200 ppm sodium hypochlorite into the feed reservoir and run as a sample Follow with 200 ppm sodium thiosulfate to deactivate the sodium hypochlorite Follow with sterile filtered laboratory grade water Use chlorine test strips to ensure that the sodium hypochlorite solution has been completely removed Place 3 hydrogen peroxide into the feed reservoir and run as a sample Follow with multiple rinses of sterile filtered laboratory grade water Use hydrogen peroxide test strips to ensure that the hydrogen peroxide solution has been completely removed The following procedure was developed at InnovaPrep for decontaminating the system with 3 Hydrogen Peroxide 1 11 12 13 14 Fill Feed Reservoir with 3 Hydrogen Peroxide until it is as full as you can get it without spilling out There should be a mound of fluid at the top of the reservoir Let stand for 5 min Place a catch cup on the shelf below the filter outlet Using a flexible tube connect a 60 ml syringe to feed reservoir drain port Set feed knob to Drain reservoir Pull 50 ml of deacon fluid out of reservoir Set feed knob to From reservoir Turn on HSC 40 Start a standard run After 5 ml of fluid have been processed simult
33. tor setting This is okay just make sure the pressure does not climb significantly when you are installing the cylinder Set the Extraction fluid valve to Run Set the valve to Off Rev 3 January 3 2010 Www innovaprep com 11 4 Screw the cylinder As soon as you feel resistance you will likely hear the gas flowing into the system and the supply pressure gauge will climb to between 800 and 1000 PSI if the tank is full Watch the Extraction fluid pressure gauge carefully as you screw in the cylinder if the pressure rises above 100 PSI or if there is already pressure on the system the pressure increases significantly unscrew the tank quickly and make sure the Extraction fluid pressure regulator is turned all the way down 5 the system pressure has equalized screw the tank in an additional sto 14 turn 6 Slowly screw in the Extraction fluid pressure regulator until the desired pressure is reached NOTE It may take several turns before the regulator engages but once it does engage the pressure will increase rapidly 7 When you are ready to pressurize the extraction fluid switch the CO valve to Run 3 8 Removing the CO Cylinder 1 Setthe Extraction fluid valve to Run 2 Setthe valve to Run 3 Unscrew the CO cylinder to turn enough to disengage the pin valve but not enough to break the o ring seal 4 Slowly turn the CO valve to CO vent When start
34. turns before the regulator engages but once it does engage the pressure can increase rapidly When you are ready to pressurize the extraction fluid switch the valve to Run 3 6 Draining the Extraction Fluid Reservoir 1 2 Attach a length of tubing from the Drain and Vent ports to a waste container If there is no pressure on the extraction fluid increase it to approximately 25 PSI using the hex key NOTE If there is significantly more than 25 PSI on the extraction fluid it may need to be lowered for the fluid to drain smoothly Turn down the Extraction fluid pressure regulator several turns and then slowly bleed some CO out of the extraction fluid reservoir by switching the valve to Reservoir vent Note once the pressure is reduced the Extraction fluid pressure gauge will not register the true pressure until the valve is returned to Run Switch the valve to Off Slowly switch the Extraction fluid valve to Drain Control the flow rate using the Extraction fluid valve When the extraction fluid has finished draining switch the Extraction fluid valve to Off Vent the Tank until it is empty by setting the CO2 knob to Reservoir vent 3 7 Installing the Cylinder 1 ps 3 Unscrew the Extraction fluid pressure regulator several turns to ensure it is set to 0 PSI NOTE If there is still pressure on the extraction fluid the Extraction fluid pressure gauge will not change when you lower the regula
35. un Standard run Settings Extract settings Foam consistency Screen shots of the appropriate menu selections are shown below in Figure 4 4throughFigure 4 8 Rev 3 January 3 2010 www innovaprep com 20 B RLIDIULIIL Rev 3 January 3 2010 Figure 4 5 Run Menu Www innovaprep com 21 Rev 3 January 3 2010 Figure 4 7 Standard Run Settings WWW innov aprep com 22 J innovapreP Figure 4 8 Extract Settings 6 Fully close the Ext fluid flowrate valve by turning it clockwise until it stops 7 Press Start on the Foam consistency menu A screen shot is shown below in Figure 4 9 JL innovaprep Figure 4 9 Foam consistency Rev 3 January 3 2010 www innovaprep com 23 8 Slowly open the Ext fluid flowrate valve until fluid starts to flow 9 Allow at least 5 mL of fluid to drain out of the retentate port to purge the system 10 Adjust the Ext fluid flowrate valve until the desired foam consistency is reached 11 Press Stop on the Foam consistency menu 12 Set the Foam timer value in seconds in the Extract settings menu using the following sequence of menu options Main menu Run Standard run Settings Extract settings gt Foam timer Foam consistency 13 Test the extraction volume by pressing Test extract in the Extraction settings menu using the following sequence of menu options Main menu Run Standard run Settings Extract settings
36. yl 2 pyrrolidone Mineral spirits Monochlorobenzene 5 Nitric acid 25 Nitric acid 6 N Nitric acid 70 Nitric acid Conc Nitric acid Nitrobenzene Nitropropane Oils mineral Pentane 25 Perchloric acid Perchloroethylene Petroleum based oils Petroleum ether 0 596 Phenol 10 Phenol 2596 Phosphoric acid N Potassium hydroxide 25 Potassium hydroxide 50 Potassium hydroxide Propanol Sample Fluidics Cellulose Ester CE Mixed Cellulose Ester ME Regenerated Cellulose RC Polysulfone PS Polyethersulfone PES Polypropylene PP difluoride PVDF Polyvinylidene 2 BPP zZ FERE C ez e ines Steel SS si L L R RIRJ R LJ R QU L RIR R LI LQ U NR NR R R R L L R UU L R R U U NR R NR RJ R U L JU UJL R NR NR NR R R L NR NR L NR R R NR NR NR NR NR NR NR R NR NR L R NR L NR NR R L L NR R NR R L E 2 AAC AIC AIC A A 1 Rev 3 January 3 2010 www innovaprep com 95 Te liE 2 EE 57 A
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