ION 01M manual ver1_4 - NPI Electronic Instruments
Contents
1. SIGNAL GROUND on the rear panel of the instrument see Figure 3 Important Always adhere to the appropriate safety measures Figure 3 Rear panel connectors of the EPMS E 07 2 Technical Data 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 and EPMS H 07 Power supply 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W EPMS E 07 External power supply for EPMS E 07 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of external power supply W x Dx H 225 mm x 210 mm x 85 mm version 1 4 page 5 ION O1M User Manual 3 ION 01M 3 1 Components of the ION 01M The following items are shipped with the ION 1M system Differential headstage with Teflon connectors Ground connector 2 4 mm banana plug Electrode connectors 1 0 mm banana plugs Manual OCC OC Oox 3 2 System Description The ION O1M amplifier is a plug in unit for the npi EPMS 07 modular system It was designed for recording signals from ionsensitive microelectrodes The system consists of a main amplifier system and a small headstage with differential input It can be used in all kind of preparations e g single cells slices or in in vivo preparations It has a low noise high common mode rejection CMR differential input equipped with ultra low bias amplifiers fA range The OUTPUT has two gains selectable by a toggle switch x10 or x100 Both inputs S sample or signa2. 12 version 1 4 page 2 ION O1M User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATI
3. C ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 4 page 3 ION O1M User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rackmount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also
4. Electronic Instruments for the Life Sciences peAsuve made OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE ION 01M IONSENSITIVE AMPLIFIER MODULE FOR EPMS SYSTEMS ION O1M VERSION 1 4 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com ION O1M User Manual Table of Contents Le Saey WSC OU ALIONNS 3525220000 7a syanantanvanced AE R E A tamageeumeniee 3 2 EPM5 07 Modular Pl g In System scrcas 4 21 General System Deseription Operation vsseondiisiashcvecacesteWaxwieredcueerd di REE 4 22 Mele VUE OWS IN oae E cata tongue acai eas aoenneweeeauanuans ete 4 2e eV ele 7 A OU IN Se enoni een raa diate tinean a ciaateian ion Rats seua eiaaaacnasen deus sanes 4 24s EPMS EOT HOUS aen E Mie ae eae 4 2 em PWR D erae N N E N ee 4 ZOO OYOK ie COUN dN e A A erga Sega aa NNEIN es 5 EFNS 7 enearo a a NE 5 EPM S EO ren EN 5 se femal Wo Ca ED i E E E ETRE 5 EPI S207 and EPM S H Ulan cet eect 5 PENS TEO eeg E E E E 5 IION OIM aeee a N 6 Sly Components o the ION OLM reia a eerhacsariavenas 6 Je ONV ENE DESCHID U Ogen a O a A bm alotoias 6 3 3 Description of the Front Panel and Operation cc cecccccccccccceessseeecceeeeeeaseeeseeeeeees 7 DAs THCAASTAS E E 10 Iae eSPe SOS a E EN E E E ee ee R 10 DOs Plecrode COMME CU OM cessa a hewn euees 11 e a e E E 12 Ja Lechma Datisicpuo a S N
5. for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing EPMS 07 cabinet with built in power supply Mains cord Fuse 2 A 1 A slow Front covers Or OF CO Cl In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing 2 3 EPMS H 07 Housing In addition to the standard power supply of the EPMS 07 the EPMS H 07 has a built in high voltage power supply This is necessary for all MVCS MVCC modules the HVA 100 HV TR1I50 and HVC 03M modules The output voltage depends on the modules in use 2 4 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow Front covers Ol Ol Ol OC OC The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the power supply 2 5 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from the one of the three connectors on the front panel of the PWR O3D to the rear panel of the respective EPMS E housing see F
6. igure 1 Figure 3 A POWER LED indicates that the PWR 03D is powered on see Figure 1 Power switch voltage selector and fuse are located at the rear panel see Figure 2 Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected version 1 4 page 4 ION O1M User Manual PWR 03D Figure 1 PWR 03D front panel view Figure 2 PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only 2 6 System Grounding EPMS 07 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration 2 pes amp EPMS E 07 The 19 cabinet is connected to the PROTECTIVE EARTH connector at the rear panel The chassis is linked to protective earth only if the PWR O03D is connected It can be connected also to the SYSTEM GROUND
7. l R reference have a capacity and OFFSET compensation The reference input can also be filtered to improve AC CMR Warning Both headstage inputs are very sensitive to static discharges and can be damaged by electrostatic charge and must therefore be handled with care Touching a grounded metal surface when changing or adjusting electrodes can avoid electrostatic discharge Always turn power off when connecting or disconnecting the headstage from the instrument version 1 4 page 6 ION O1M User Manual 3 3 Description of the Front Panel and Operation ION O1M npi O Deea Mop O INTERNAL MODES OUTPUT RANGE RAMP OUTPUT S OUTPUT S R OUTPUT R INPUT x10mV mV x10mV O O Figure 4 Front panel view of the ION 1M In the following description of the front panel elements each element has a number that is related to that in Figure 4 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given version 1 4 page 7 ION O1M User Manual 1 CCOMP R potentiometer 10 turn potentiometer for setting the capacity compensation for the reference electrode 2 OFFSET R potentiometer 10 turn potentiometer for setting the OFFSET compensation for the reference electrode range 100 mV 3 FILTER R potentiometer 10 turn potentiometer for setting the low pass FILTER for the reference electrode The
8. low pass filter improves AC CMR range 100 Hz 10 kHz 4 OUTPUT RANGE switch Switch for setting the range x10 or x 100 of the OUTPUT of the S R signal 5 OUTPUT R connector BNC connector providing the voltage at the reference electrode amplified by ten mV 6 OUTPUT S R connector BNC connector providing the voltage at the sample electrode minus the signal at the reference electrode The amplification factor is set by 4 7 OUTPUT S connector BNC connector providing the voltage at the sample signal electrode amplified by ten mV 8 INTERNAL MODES switch Switch for selecting the RAMP INPUT BNC connector 9 or the OPERATE switch 12 9 RAMP INPUT connector BNC connector for connecting a ramp 10 V max for measuring the electrode resistance The ramp signal is fed into a capacitor differentiated and generates a rectangular signal at the electrode resistance This signal is provided at the OUTPUT S connector and it s amplitude is proportional to the electrode resistance 10 PROBE connector Connector for connecting the headstage 11 CMR ADJ trimpot Trimpot for adjusting the CMR CMR is adjusted by linking the same rectangular signal to both inputs S and R watching the OUTPUT S R and turning the trimpot until no signal can be seen at the oscilloscope 12 OPERATE switch Switch for selecting an internal generated ramp 1 5 V s for electrode resistance testing see also 9 or for showing the elec
9. mple electrode and a reference electrode which are connected to high impedance buffers input resistance better than 10 Q in the headstage are required for detecting signals from ion sensitive electrodes In addition the bath surrounding the cell must have a ground connection AgAgCl pellet or Agar bridge see Figure 7 It is important that there is always this pathway to ground although the bias current is in the fA range to avoid saturation effects The electrodes can be connected by thin flexible unshielded wires e g inside a Faraday cage to the headstage Important The headstage inputs are very sensitive and can be damaged by electrostatic charge Therefore be handled with great care Touching a grounded metal surface when changing or adjusting electrodes can avoid electrostatic discharge The wanted signal is available at the S R connector and can be amplified by 10 or by 100 version 1 4 page 11 4 Literature ION O1M User Manual Polder H R M Weskamp K Linz and R Meyer Voltage Clamp and Patch Clamp Techniques Chapter 3 4 pp 272 323 in Dhein St Mohr F W Delmar M Eds Practical Methods in Cardiovascular Research Springer Heidelberg 2004 Schwarz W and J Rettinger 2003 Foundations of Electrophysiology Second Edition Shaker Verlag Aachen 2003 Voipio J M Pasternack and K Macleod 1996 Ion sensitive microelectrodes Chapter 11 in Ogden D ed 1996 Microelectrode Techniques The Plym
10. outh Workshop Handbook Second Edition The Company of Biologists Ltd Cambridge 5 Technical Data ION 0O1M Input range Input impedance Differential input FILTER reference electrode GAIN S R OFFSET compensation CAPACITANCE compensation Output Digital display Size version 1 4 1000 mV gt 10 TQ related to ground cmr gt 120 dB gain 100 1 pole low pass range 100 Hz 10 kHz x10 x100 selected by toggle switch range 100 mV range 0 30 pF range 12 V into 1 kQ 1 V into 50 Q XXX X mV or XX XX GQ sample electrode or XXX X MQ reference electrode front panel 12 HP 60 6 mm x 3U 128 5 mm 7 175 mm deep page 12
11. s Two resistors are provided to test the amplifier Both inputs can be connected to a function generator via a voltage divider 1000 which is also supplied For testing and CMR adjustment apply a rectangular signal e g 2 V to the voltage divider This way identical small signal will be applied to both inputs and by adjusting filters capacity compensations and CMR an ideally straight signal will appear at OUTPUT S R Figure 6 headstage resistors Test resistor for sample electrode 1 GQ Test resistor for reference electrode 10 MQ Note Connection of these resistors to ground protects the inputs from damage by electrostatic discharge version 1 4 page 10 ION O1M User Manual 3 6 Electrode connection ELECTROSTATIC DISCHARGE SENSITIVITY This integrated circuit can be damaged by ESD Burr Brown recommends that all integrated circuits be handled with appropriate precautions Failure to observe proper handling and installation procedures can cause damage ESD damage can range trom subtle performance degradation to complete device failure Precision integrated circuits may be more susceptible to damage because very small parametric changes could cause the device not to meet its published specifications Solution round sample Elschroade Reference Elechode Figure 7 Equivalent circuit of ionsensitive measurement The principle of operation is shown in Figure 7 Two electrodes an active ion exchanger sa
12. trode resistance at display 17 The electrode to be tested is selected by switch 16 version 1 4 page 8 ION O1M User Manual 13 OFFSET S potentiometer 10 turn potentiometer for setting the OFFSET compensation for the sample electrode range 100 mV 14 CCOMPS potentiometer 10 turn potentiometer for setting the capacity compensation for the sample electrode 145 GQ MQ mvV LEDs LEDs indicating the unit of display 17 16 DISPLAY MODE switch Switch for selecting the electrode whose signal that is displayed at 17 voltage or resistance S Voltage at the sample electrode or resistance of the sample electrode S R Signal at the sample electrode minus signal at the reference electrode R Voltage at the reference electrode or resistance of the reference electrode Hint If electrode resistance test is selected and the switch is in S R position the voltage difference S R is shown anyway and the GQ MQ mV LEDs are switched off 17 Digital display Display showing the voltage selected by switch 16 or the resistance of the sample electrode or the resistance of the reference electrode XXX X mV or XX XX GQ sample electrode or XXX X MQ reference electrode version 1 4 page 9 ION O1M User Manual 3 4 Headstage Figure 5 headstage electrode mount connector for the sample ionsensitive electrode S connector for the reference electrode 1 mm jack R connector for ground GND ee 3 5 Test resistor
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