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DR 5000 User Manual

1. Formula Enter the next calibration formula C2 or Cn 5 Highlight C2 Off and press Edit 6 Select C2 Off again and press Edit The display switches to C2 On 7 Highlight the next line C2 to define the formula and press Edit In addition to the keys described in Table 11 only one other function is available 77 Advanced Operations F1 5 0380 U1 1 0000 U2 3 2840 Select Conc key If an already defined formula in this case C1 is to be taken into account in the formula for C2 press Select conc Enter the number of the formula e g 1 for C1 and press OK to confirm Cn can now be linked with a mathematical operation Note The Cn concentrations that are to be calculated are numbered in sequence C1 C2 C3 etc Note When the first Cn formula has been defined the Formula list is automatically extended by Cn 1 1 Highlight the Variables line in the data overview and press Edit 2 Select the variable to be edited press Edit and use the alphanumeric keypad to enter the data specified in the working procedure for F1 F2 11 U1 etc Press OK to confirm each entry Abbreviation of Variables F1 Factor 1 F2 Factor 2 21 Wavelength 1 U1 Conversion Factor 1 for the first chemical form U2 Conversion Factor 2 for the further chemical form etc 6 1 2 5 Save a free programming user program 78 1 Press Store to save t
2. New Number Press New Number to enter a new factor or constant 76 Advanced Operations Table 11 Edit formula key descriptions continued Screen Key Description Press x to enter a mathematical operation Math operator Select the operation and press OK to confirm The available choice of mathematical operations depends on the defined formula This means that functions such as or In log etc are only active if a term in parentheses or the calculation of a logarithm is mathematically permissible in the defined formula this also applies to the basic mathematical operations The following mathematical operations are available e Addition e Subtraction x e Division e x Multiplication e Exponent e Ln Natural logarithm e Log Common logarithm User aie Press gt lt to include logic statements links conditions in Conditional operator the formula The following functions are available Equal to lt Less than gt Greater than lt Less than or equal to gt Greater than or equal to IF THEN ELSE When the evaluation formula C1 has been entered completely press OK to confirm Press OK again to return to the Formula display When the formula C1 has been entered and confirmed the parameter name upper and lower limits of the measuring range and display result yes no can be entered gt lt
3. The most frequently used tests methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify their selection To add Stored Programs and or User Programs to the favorites list or the favorite programs see section 6 1 4 on page 79 1 Press Favorite Programs in the Main Menu The Favorite Programs list will appear 2 Highlight the selection by pressing it Note Use the scroll bar to scroll through the list quickly Note Press Select by Number to search for the program by number Use the alphanumeric keypad to enter the test number program number and confirm the input by pressing OK 3 Press Start 1 Press Favorite Programs in the Main Menu The Favorite Programs list will appear 2 Highlight the selection by pressing it Note Use the scroll bar to scroll through the list quickly Note Press Select by Number to search for the program by number Use the alphanumeric keypad to enter the test number program number and confirm the input by pressing OK 3 Press Remove Program and press OK to confirm Note If a Favorite Programs is deleted it will stay in the User Programs or Stored Programs Note If the stored program is deleted in User Programs it will also be deleted in Favorites Programs 81 Advanced Operations 6 3 Standard Addition monitoring checking results 82 The accuracy of measured values their correspondence with the actual
4. 42 Standard Operations 10 Aluminum Alumin 15 SEP 2006 Options Zero Read Reading Mode Data Single Z Dilution Standard Factor Off Additions Standard Chemica Form Adjust Off Reagent Blank Save as off User Program 14 49 13 Instrument Setup Table 4 Stored programs options continued Description Store Off On With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored Trans Conc Abs To switch to transmittance concentration or absorbance readings Send Data icon Send Data To send Data to a printer computer or USB memory stick Type A Timer icon This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program Reading Mode Dilution Factor Off On Single Reading Mode A reading is only displayed after a measurement has been carried out press Read standard setting see section 5 4 4 1 on page 45 Continuous Reading Mode After the zero measurement all readings are displayed automatically and continuously see section 5 4 4 2 on page 45 A corrective dilution factor can be entered in order to take account of certain properties The number entered at the
5. Single Wavelength 0 921 25 SEP 2006 09 14 24 320 nm a Options Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Note The Read key is only active after the zero measurement has been completed Insert the sample cuvette cell into the cuvette sample cell holder Press Read For data storage see section 5 3 2 on page 38 6 4 3 Take single wavelength measurements continuous readings Single Wavelength 0 916 s 25 SEP 2006 09 15 28 Dd Options Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Note In the reading mode Continuous only the Zero key is shown to start the reading The reading sequence is started automatically Insert the sample cuvette cell into the cuvette sample cell holder Press Options and then the Store icon to store the displayed data in the Data Log Note For data storage see section 5 3 2 on page 38 89 Advanced Operations 6 5 Multi Wavelength mode measurements at more than one wavelength In the multi wavelength mode absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums differences and relationships Absorbance measures the amount of light absorbed by the sample in units of absorbance Transmittance measures the percent of the original light that passes through the sample and reaches the detector Turning on the conce
6. Average 131 49 n Advanced Operations 5 Insert sample cuvette cell into the cell compartment Close the cell compartment 6 Press Start 7 The result will be displayed Thirty readings are averaged for the blank The Average and Std Deviation are calculated from 100 consecutive absorbance readings Noise Sample Reference Note Failings and passings will be defined by the user 8 Press PC amp Printer icon to send data to a PC or Printer 9 Press Cancel to return to Optical Checks 109 Advanced Operations 6 8 3 3 Stray light check The Stray Light test is used to measure the stray light in the instrument at 220 0 nm and 340 0 nm SEE Wavelength 1 Press Stray Light Check in the Optical Checks menu 2 Press A to select the wavelength 340 0 nm C 220 0 nm 3 Make a choice and confirm with OK COMER Oo tics Abanke E Stray Light Check 4 Press Zero Wavelength 340 0 nm 9 ti al Oka y A kl Stray Light Check 5 Insert the sample cuvette cell or reference filter into the cell compartment Close the cell compartment Press Start Wavelength 340 0 nm 0 0000 Abs 110 Optics Chae A Stray Light Check wavelength 340 0 nm 0 7710 Abs Average 0 7707 Abs Std Deviation 0 0005 Abs 6 8 3 4 Absorbance check e tics ede Ll p Absorbance Check Wavelength 546 1 nm e tic Abanke E Absorbance Check wavelength 546 1 nm
7. wavelength Scan The Store Data list will be displayed 15 SEP 06 14 20 33 Scan 7 400 600 nm 4 001 nm A cock cte me aie ACI oi oye A meee 2 Press Store to save the current scan to the hi ghli ghted 18 SEP 06 09 19 40 Scan 8 400 600 nm 001 nm numbered line A scan can also be overwritten 18 SEP 06 09 26 44 Scan 9 400 600 nm 4 001 nm 18 SEP 06 09 29 14 Scan 10 400 600 nm 4 001 nm Scan 11 Cancel Wavelength Scan 1 Press Recall Data in the Main Menu 14 20 33 400 600 nm 001 nm 6 09 19 40 400 600 nm 001 nm l 09 26 44 b Ifa program is already in progress press 400 600 nm 001 nm Options gt More gt Recall Data a Select Wavelength Scan or Time Course to recall data 09 29 14 400 600 nm 001 nm D6 10 01 32 400 600 nm 4 001 nm Main Menu Table Graph Options 38 Standard Operations Recall Data Wavelength Scan ae 2 Press Graph to look at details Note Press View Summary to return to the Recall Data list A 0 200 400 600 Integral 279 00 View a Summary Options Wavelength Scan P 3 Press Table to look at details E n HM T told SEO Note Press View Summary to return to the Recall Data list 0 494 i401 0 476 0 504 1403 0 500 Peak 0 500 i 405 0 448 0 453 1407 0 472 0 475 409 0 503 Peak 0 481 i411 0 481 Valley 7 Integral 279 00 View Summary Options 5 3 2 3 Send data from wavelength scan or time cour
8. HACH Company 2007 2008 All rights reserved Printed in Germany D DOC022 53 00654 DR 5000 USER MANUAL January 2008 Edition 2 Table of Contents Section 1 Specifications eei ti ub tet da ta a ee e dug 7 Section 2 General Information sssssssssssssssssssssee eene I en net nnn nennen rtr nn nnl sienne n nennen 9 2 1 Safety information gen d Leni ede O Fra deo e aSa benedeni edi Bret 9 2 1 1 Use of hazard information sss ensem nn a nan nnns nn nn nnne nennen nennen 9 2 1 2 Precautionary labels sonerie needed ad aee ee p pei da a eee ea a b be dea agi ku een ee eia a ud ue 9 ZN Clas 1 TL P ULT 10 2 1 4 Chemical and Biological Safety sssssssssssssseseeeeen enne nemen nennen nnne nnn nens 10 2 1 5 Source Earmip Safely iii tbi Ce eade able e ie em bt cd re e bue d 10 2 2 Qvervlew of PRODUCE iei itera ERR A iaa 11 Section 3 Installation roe uet lee a erit dua cul p ede RR ede de eee 13 3 1 Unpacikthe InSTUMENE ERE 13 3 2 Operating environment E 13 3 9 CONMOCUON ui A A ti Dawes al tb ARA dr 14 3 4 Cell compartment Multi Cell Holder 100 mm Cell Adapter optional oooooococnncnnnnnnncocoooconcccnnnncncnnnnnnannoncnnno 15 3 4 1100 mMm adapter optional 2 terr peret ita lado Peg d ec dia 16 3 4 2 Installation Multi Cell Holder sse Ie enne nennen nnne nn nn sls nnne nnne nennen 17 3 5 BEAM P
9. Favorite Programs User Programs Reading barcode Please wait Wavelength Scan Instrument Setup Recall ciate et Data Checks 000 nm mg l Reading Nickel Options 13 JAM 2005 08 07 08 Exit Zero 5 5 2 Select the measuring range Stored Programs Select by Number Start Permanent Note The cell compartment must be closed before a measurement is made 1 The instrument needs to be at the Main Menu 2 Prepare the barcode 13 mm cuvette vial in accordance with the working procedure and insert the cuvette vial in cell compartment 1 and close the cell compartment When a coded cuvette vial is placed in cell compartment 1 Figure 7 the corresponding measurement program is automatically activated in the Main Menu The measurement is started automatically and the results are displayed To evaluate other cuvette vial tests and other parameters insert the prepared cuvette vial into the cell compartment and read the result Some tests can be used for different measuring ranges After the sample cuvette vial has been inserted a list of the different measuring ranges is displayed Select the required measuring range by highlighting the appropriate line Press Start Permanent if this measuring range is to apply to all subsequent measurements Changing the standard setting In the result display press Options More Permanent On The key will change
10. Saale Memory is displayed Connect a USB stick in order to store the data Press OK to confirm and press Restore again Please insert USB Memory Restore 4 Press OK to confirm after the message Instrument Backup from S N XXXXXXX Restore is displayed Instrument Backup from S N 1175848 Restore Cancel OK Restore 5 After the backup start the instrument again Please switch the instrument off and on for using the restored Backup 119 Advanced Operations 120 Section 7 Maintenance 7 1 Cleaning requirements 7 1 1 Spectrophotometer 7 1 2 Display 7 1 3 Cuvettes sample cells CAUTION Potential Chemical Biological Eye and Skin Hazards Only qualified personnel should conduct the tasks described in this section of the manual Important Note Remove any cuvettes cells that are still in the instrument and dispose of them or their contents using an approved disposal method CAUTION Potential Pinch Eye Burn and Chemical Hazards Always disconnect power from the instrument before attempting any cleaning operations Important Note Under no circumstances should the instrument display or the accessories be cleaned with solvents such as white spirit acetone etc e Clean the enclosure cuvette sample cell compartments and all accessories with a soft damp cloth A mild soap solution can also be used Do not get excess water in the cuvette sample cell compartments Do not ins
11. 05 0 000 mg L AI Main Filter View Menu Off Details Options 5 2 6 3 HACH Data Trans Instp mant Omat PC amp Printer Printer Deskjet 6500 A PC Connected USB Memory Connected Keyboard Connected 7T Press Paper to select the paper size Select between e Letter e Legal e Executive A4 8 Press OK to confirm Note Press OK again to return to the PC amp Printer menu 1 Press Recall Data in the Main Menu 2 Select the data source where the data to be printed are stored 3 Alistis displayed Data can be filtered For more information see section 5 3 1 2 on page 35 4 Press the PC amp Printer icon to send the data table curve immediately to the printer 5 Highlight Single point or Filtered data or All data and press OK to confirm Sending Data is displayed until the data have been printed The optional HACH Data Trans software must be installed on the PC for the subsequent to process for measurement data 1 Press PC amp Printer in the Instrument Setup 2 Select PC 3 Press Setup to display the PC Setup screen For further installation instructions refer to the HACH Data Trans user manual 31 Standard Operations 4 Select Auto Send On to send all measured data automatically Auto Send to the PC On Remote Note If Auto Send Off is selected the PC amp Printer icon must be pressed in order to send data to the PC Note T
12. 06 1 225 7784 info hach lange hu www hach lange hu HACH LANGE D O O Fajfarjeva 15 SI 1230 Dom ale Tel 386 0 59 051 000 Fax 386 0 59 051 010 info hach lange si www hach lange si Repair Service in the United States HACH Company Ames Service 100 Dayton Avenue Ames lowa 50010 Tel 800 227 4224 U S A only Fax 515 232 3835 HACH LANGE LTD Pacific Way Salford GB Manchester M50 1DL Tel 44 0 161 872 14 87 Fax 44 0 161 848 73 24 info hach lange co uk www hach lange co uk HACH LANGE FRANCE S A S 33 Rue du Ballon F 93165 Noisy Le Grand T l 33 0 1 48 15 68 70 Fax 33 0 1 48 15 80 00 info hach lange fr www hach lange fr HACH LANGE AB Vinthundsv gen 159A SE 128 62 Sk ndal Tel 46 0 8 7 98 05 00 Fax 46 0 8 7 98 05 30 info hach lange se www hach lange se HACH LANGE SP ZO O ul Opolska 143 a PL 52 013 Wroctaw Tel 48 0 71 342 10 83 Fax 48 0 71 342 10 79 info hach lange pl www hach lange pl HACH LANGE S R L Str Leonida nr 13 Sector 2 RO 020555 Bucuresti Tel 40 0 21 201 92 43 Fax 40 0 21 201 92 43 info hach lange ro www hach lange ro HACH LANGE E N E AuAidoc 27 GR 115 27 AB va TnA 30 210 7777038 Fax 30 210 7777976 info hach lange gr www hach lange gr Repair Service in Canada Hach Sales amp Service Canada Ltd 1313 Border Street Unit 34 Winnipeg Manitoba R3H 0X4 Tel 800 665 7635 Canada only Tel 204 632 5598 Fax 204 694
13. 35 Instrument Setup Basic data of the instrument see section 5 2 on page 23 43 Standard Operations 5 4 3 Use of program timers 10 atrio del ds de Mm Timer Invert Timer 01 00 Shake Timer 00 30 Timer 15 00 General Timer 11 34 5 Cancel OK E 10 Al eel 22 nm Timer mm ss Boe BET m EA E E EA 5 Cancel OK y 5 4 4 Set the reading mode Optir Reading Mode Single Reading C Continuous Readings 44 Some procedures do not require the use of timers Other procedures require several timers These timers are pre programmed into each Stored Program along with a description of the activity to be performed during the timed period 1 Press the Timer icon on the display 2 Press OK to start the first timer The timer will count down on the screen 3 To start the next timed activity for the Stored Program press the Timer icon and OK Note Press Close to view the measurement screen while the timer is running The time will be shown left side bottom instead of the date Note Press Cancel to stop the timer midway through the countdown Note The Timer will beep when the end of the running time is reached A general purpose timer is also available in many programs When the timer icon is visible press the icon and select General Timer A new screen will appear Enter th
14. 5 2 Complete a measurement in the multi wavelength mode Multi Wavelength VIS 500 nm 2 575 Aaoo Asoo 2 361 0 214 25 SEP 2006 11 05 04 Main Menu Zero Options Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Note The Read key does not become active until the zero measurement has been completed Insert the sample cuvette cell into the cuvette sample cell holder Press Read Note The UV lamp is not switched on until a wavelength in the UV spectrum has been entered and the blank reading has been started During the warm up phase of the UV lamp the message Warming up is displayed and the UV lamp symbol flashes As soon as the UV lamp is ready the blank reading is carried out Note Zero and Read are disabled until the cell compartment is closed For data storage see section 5 3 1 1 on page 35 93 Advanced Operations 6 6 Wavelength Scan mode recording of absorbance and transmittance spectrums In the wavelength scan mode the absorbance of the light in a solution over a defined wavelength spectrum is measured The measurement results can be displayed as a curve as percent transmittance T or as absorbance Abs The collected data can be printed as a table or a curve The data are available for formatting changes These include automatic scaling and zoom functions Maximum and minimum values are determined and shown as a table The cursor can be mov
15. 50 00 ma L al 7 Press the keys to enter the standard addition volumes Use the alphanumeric keypad to enter the new data and press OK Standard Additions mL Yo Standard Recovery e The first column shows the standard addition volume 0 mL stands for a sample containing no added standard Description of the table of readings e The second column shows the reading of the samples with and without added standard e The third column shows the detection rate of the added standard Note The black highlighted line is active 8 The reading of the sample in the instrument without added standard is automatically shown under 0 mL 9 Prepare the standard addition solution as described in the procedure 10 Use the arrow keys to select the first standard addition volume in the table and insert the cuvette cell with the corresponding volume of added standard into the cell compartment Press Read Repeat the procedure from point 8 with all the other standard addition solutions 85 Advanced Operations Standard Additions Approx Conc 0 228 mg L AP mg L 0 0 Curve Fit r 0 9993 86 11 12 After all the standard addition solutions have been measured press Graph The regression line through the standard addition data points is displayed The correlation coefficient r2 indicates how close the data points are to the line If the correlation coefficient 1 the curve is linear The co
16. 8 5 on page 115 123 Maintenance AA i y AMA y NAAA Figure 8 Lamp compartment 1 Tungsten lamp 5 Plug contacts for fan 2 Spring 6 Plug contact for deuterium lamp 3 Screws 7 Plug contact for tungsten lamp with grip and cable 4 Deuterium lamp lamp socket 7 3 Fuse replacement 124 DANGER Certain electrical circuits within this equipment are protected by fuses against over current conditions For continued protection against a risk of fire replace fuses only with the same type and rating specified DANGER Failed fuses are generally an indication of a problem with the equipment If fuse failure continues contact a service representative for instructions on how to return the equipment for repair Do not attempt to repair the equipment yourself 1 Switch the instrument off 2 Unplug the power cord to remove all power from the instrument 7 4 Filter pad maintenance 7 4 1 Filter pad replacement Maintenance 3 Pull out the plastic base over the power cable socket 4 Remove the defective fuse see Figure 1 on page 14 5 Insert new fuse T2 A H 250 V 6 Replace the plastic base 7 Plug in the power cord 8 Switch the instrument on To determine when the filter mat needs to be replaced inspect the filter mat every 3 6 months in a relatively dust free environment this inter
17. Adapter 1 100 mm rectangular cell Installation 3 4 2 Installation Multi Cell Holder Assembly of the Multi Cell Holder 1 Open the cell compartment 2 Identify the correct opening for the selected cell type in the Multi Cell Holder 3 Insert the Multi Cell Holder in the cell compartment in such a way that the name of the selected cell type can be read directly and the cell opening is at the front Secure the holder with two locking screws Figure 5 Figure 5 Installation Multi Cell Holder Installation 3 5 Beam path Figure 6 shows the beam path of the DR 5000 Figure 6 Beam path 1 Deuterium lamp 10 Exit slit 2 Lamp selection mirror 11 Cell compartment 3 Tungsten lamp 12 Lens 4 Mirror 13 Round cuvette vial compartment 5 Grating 14 Measurement element 6 Reference element 15 Monochromator 7 Mirror 16 Mirror 8 Splitter mirror 17 Mirror 9 Filter 18 Entrance slit 18 Section4 Start Up 4 1 Power the instrument on and off 4 2 Language selection Main Language English Deutsch 4 3 Self Check m DR 5000 Self Check SYSTEM TESTS LAMP TESTS CALIBRATION FILTER ADJUSTMENT VOLTAGE TESTS Please keep lid closed 85 1 Plug in power supply 2 Close the empty cell compartment 3 Turn the instrument on by pressing the power switch on the back Note Do not turn the instrument off and on in rapid succession
18. Follow the entire procedure using a known standard for the sample 2 After reading the concentration press Options gt More gt Standard Adjust 3 If Standard Adjust is set to Off turn it On The Current Reading will show the concentration The box on the right will show the default standard value for the test as mentioned in the procedure 4 Ifthe measurement used a standard concentration that is different from the one displayed in the box press the box on the right to enter a different standard value and enter the new value Press OK to confirm 10 Aluminum Alumin 0127 7 m 7 18 SEP 2006 15 21 32 e a Options 5 4 7 Set the chemical form Chemical Form i east Default ale 5 4 7 1 Change of the default setting of the chemical form Standard Operations Press Adjust to enable the Standard Adjust The Standard Adjust icon will appear Note The adjustment must be within certain limits which vary with each program The allowable percentage is shown after Adjustment Note When a Standard Adjust is in effect the Standard Adjust icon will appear on the display Some Stored Programs allow a variety of chemical forms to be selected Press the unit e g mg L or the chemical representation of the evaluation form e g AI3 A list of available evaluation forms is displayed Select the required form by pressing the corresponding entry in the list Note When the program is exited t
19. More gt Send Data to send the data to a USB memory Stick or to a printer e When a printer is connected select how to send the data to the printer graph table or both graph and table When a USB memory stick is connected the files will be automatically sent as CSV files Comma Separated Value to a file WLData Wavelength Scan Data or TCData Time Course Data The file name will be formatted as ScanData Year Month Day Hour Minute Second CSV Wavelength Scan Data or The file name will be formatted as TCYear Month Day Hour Minute Second CSV Time Course Data For further processing use a spreadsheet program Standard Operations 5 3 2 4 Delete stored data from wavelength scan or time course Recall Data Wavelength Scan 1 Press Recall Data from the Main Menu and then Wavelength 15 SEP 06 14 20 33 ions gt Bice rasa rare pete ae arg Scan or Time Course or Options gt More gt Recall Data 18 SEP 06 09 19 40 ie me Scan8 400 600 nm 001 nm A listing of the stored data is displayed 18 SEP 06 09 26 44 Scans 29006001001 nm 2 Highlight any data to delete 18 SEP 06 09 29 14 Delete Scan 10 400 600 nm 001 nm w Options PE 3 Press Delete in the Options menu and press OK to confirm can 11 400 600 nm 4 001 nm 41 Standard Operations 5 4 Stored Programs The instrument contains more than 200 programmed procedures They can be accessed through the Stored Programs menu 5 4 1
20. Options 5 4 10 Add stored programs to the favorite programs list The Favorites menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs and User Programs 1 Press Stored Programs in the Main Menu The Stored Programs list will appear Program 510 chlorine Joone 2 Highlight the selection by pressing it or Select by Number to search for the program by number Add to Favorites 3 Press Add to Favorites and press OK to confirm The program can now be selected from Favorite Programs menu in the Main Menu 49 Standard Operations 5 5 Barcode Programs A special barcode reader in cell compartment 1 automatically reads the barcode on the 13 mm cuvette vial as the cuvette vial completes a single rotation The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with the help of the stored factors In addition measured values are recorded at 10 different positions during a rotation A special outlier elimination program is run and then the average of the measured values is calculated Cuvette vial errors and soiling are recognized and highly precise results are obtained Figure 7 Cell compartment 1 1 Cell compartment 1 for 13 mm barcode cuvettes or 16 mm vials 50 Standard Operations 5 5 1 Complete a barcode 13 mm test vial Stored Programs
21. Select a saved test method enter user specific basic data Stored Programs 4 Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with program numbers 10 Aluminum Alumin 0 800 mg L 9 Aluminum ECR 0 250 mg L The Stored Programs list will appear 5 Highlight the required test Note Select the program number by name or use the arrow keys to scroll through the list quickly and highlight the program or press Select by number to search for a specific program number Use the Main Selectby Program alphanumeric keypad to enter the test number and press OK Menu Number Options 6 Press Start to run the program After a program is selected the screen for that parameter will appear Note All corresponding data wavelength factors and constants are already preset 7 Follow the chemical procedures described in the corresponding Procedures Manual 5 4 2 Stored program options 1 From the Main Menu select Stored Programs Select the necessary method and press Start 2 Press Options for Parameter Setup Refer to Table 4 for stored program descriptions 10 Aluminum Alumin Reading Mode Single E Dilution Standard Factor Off Additions Standard Chemical Form Adjust Off AI Reagent Blank Save as off User Program 15 SEP 2006 14 49 13 Instrument Zero Read Return Setup Table 4 Stored programs options Options Description More For further Options
22. Setup 7 The Sample ID is activated Each Sample ID is automatically numbered in ascending order after a measurement The number is shown in parentheses behind the Sample ID To remove a Sample ID highlight the ID and press Delete Standard Operations There are two ways to enter or change a Sample ID in the Measurement Mode Option 1 In the result display press Options gt More gt Instrument Setup If a Sample ID is already assigned select the Sample ID symbol in the result display 10 Apter Aoi ure JAN 22 nm i Sample ID Option 2 With an USB keyboard with US keyboard layout or a scanner a Sample ID can be directly entered in the result display Press Date amp Time in the Instrument Setup The date and time are subdivided over a number of fields Press the appropriate field and use the arrow keys to change the value Press OK to confirm The instrument will return to Instrument Setup AS Display amp Sound 1 Press Display amp Sound in the Instrument Setup The following options will be displayed e Display Contrast Agjusts the display brightness to suit EE lighting conditions Screen touch e Screen touch Activates Deactivates a short beep each time the screen is pressed Default off MV Reading done Timer Short Long 4 Reading done Activates Deactivates a sound when a Cancel OK reading is complete Default short beep every time a reading
23. and reagents can be dangerous Users of this product are advised to familiarize themselves with safety procedures and the correct use of chemicals and to carefully read all relevant Material Safety Data Sheets Normal operation of this instrument may involve the use of hazardous chemicals or biologically harmful samples e The user must observe all cautionary information printed on the original solution containers and safety data sheet prior to their use e All waste solutions must be disposed in accordance with local and national law e The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace The source lamp operate at high temperatures e To avoid a possible electric shock disconnect the instrument from the power source before servicing the lamp Make sure that the instrument cannot be accidentally switched on again CAUTION The UV lamp generates UV light Do not look directly at an operating lamp without wearing UV protective eye glasses Important Note To prevent a possible burn allow the lamp to cool at least 30 minutes before handling 2 2 Overview of product General Information The DR 5000 Spectrophotometer is a complete scanning UV VIS spectrophotometer with a wavelength range of 190 to 1100 nm The instrument comes with a complete set of application programs and multi language support The DR 5000 Spectrophotometer cont
24. and then New Content and definition of the keys set ESA Process 2 key Zeroing 1 Press the Z key to program a zero measurement Confirm with OK 2 Press New and then Zeroing and use the alphanumeric zeroing Reading keypad to enter the wavelength at which the zero measurement is to be completed Press OK and confirm the input by pressing Process er OK again 3 If zero measurements are to be carried out at a number of wavelengths repeat the above two steps for each wavelength Note The entered measurement sequence is displayed 73 Advanced Operations User a a oct Measurement Process Process badd Timer Cancel OK i User a oct Measurement Process Risco roe P 74 Press the Process Timer key to enter any waiting reaction or handling times that have to be taken into account Use the alphanumeric keypad to enter the time Press OK and confirm the input by pressing OK again Note This time is integrated into the measurement process Note The entered measurement sequence is displayed R key Reading 1 Press the R key to program a measurement of the substance that is to be analyzed Confirm with OK Press New and then Reading and use the alphanumeric keypad to enter the wavelength at which the measurement is to be completed Press OK and confirm the input by pressin
25. be changed 1 Highlight the Measurement Process line in the data overview and press Edit 2 Press Edit select the sequence to be edited and press Delete 3 Press New and use the alphanumeric keypad to enter the process indicated in the working procedure Enter the Formula Concentration Units Designation Measuring Ranges For detailed information on entering the Formula see section 6 1 2 on page 72 Check the working procedure in advance to determine whether this information needs to be changed 1 Highlight the Formula line in the data overview and press Edit 2 Select the formula press Edit and use the alphanumeric keypad to enter the data specified in the working procedure for C1 C2 units name measuring range limits etc 55 Standard Operations ESSE 56 E Cancel E OK Timers Timer 00 00 Loa Timer 00 00 Timer 00 00 a 4 Timer 00 00 WS Enter the Variables Factors Wavelength and Conversion Factors For detailed information on entering the Variables see section 6 1 2 on page 72 Check the working procedure in advance to determine whether this point needs to be changed 1 Highlight the Variables line in the data overview and press Edit 2 Select the variable press Edit and use the alphanumeric keypad to enter the data specified in the working procedure for F1 F2 21 U1 etc Confirm each entry with OK Abbreviation of Variables F1 Facto
26. chemical form and calibration equation More information on Free Programming parameters see section 6 1 2 on page 72 Table 8 Program descriptions Program Type Description Single Wavelength Measurements at a defined wavelength Multi Wavelength In the Multi Wavelength mode absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums differences and relationships 62 This is an advanced form of programming for original user developed methods In Free Free Programming Programming the user defines the measurement process variables and calculations involved in obtaining a reading 6 1 1 1 Single wavelength settings Units lt Wavelength A nm User Lar nen m m Concentration Resolution Advanced Operations If the Single Wavelength mode is selected the following parameters can be defined Units Select the required unit from the list and press Next Note Units of measure not included in this list can be added in the edit program under Program Options Edit Select Units Edit and then New Wavelength single wavelength program type Use the alphanumeric keypad to enter the measurement wavelength The entered wavelength must be in the range from 190 1100 nm Press Next to proceed Concentration resolution number of decimal places Select the required number of deci
27. dilution factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off Note When a dilution is in effect the dilution icon will appear on the display Standard Addition This enables the accuracy of the measurements to be checked The working procedure for a test parameter contains a detailed explanation of how to use this function Standard Adjust The working procedure for a test parameter indicates whether a standard adjustment is necessary and if so how to proceed Chemical Form Some of the stored tests methods allow to select the chemical form and the measuring range Reagent Blank Some of the stored tests methods include the Reagent Blank function This enables the reagent blank value to be added to or subtracted from the subsequent readings The reagent blank value shifts the calibration curve along the y axis without changing the shape or gradient of the curve The effect corresponds to a y axis intercept of the calibration straight line This is made clear by the following equation Concentration Conc factor Abs reagent blank value Save as User Program To store the selected parameters as a User Program see section 6 1 on page 61 Recall Data Call up saved measurement data wavelength scans or time courses see section 5 3 on page
28. display shows the chosen Operator ID 6 Press OK The instrument will return to the Instrument Setup Screen and show the selected operator identifier 7 The chosen Operator ID is activated Note Press Delete to remove an Operator ID from the list Note Alternatively enter or change an Operator ID in measurement mode In the results screen press Options gt More gt Instrument Setup or if an Operator ID is already assigned select the Operator ID symbol immediately in the results screen 23 Standard Operations 5 2 2 Sample ID Instn ot Lots t Sample ID Add M Number hd 03 de New Delete OK 24 Use this option to enter up to 30 Sample Identification tags up to 13 characters each into the instrument Sample IDs can be used to specify the sample location or other sample specific information 1 Press Sample ID in the Instrument Setup 2 Press New to enter a new Sample ID 3 Use the alphanumeric keypad to enter a new Sample ID Note Ifa USB Barcode handset scanner see Section 9 on page 129 is connected Sample IDs can also be scanned 4 Press OK to confirm 5 To number the Sample IDs sequentially e g Inflow 01 etc select Add Number e Use the arrow keys to specify the first number of the sequence e Use the key between the arrow keys to enter the first number of the sequence using the alphanumeric keypad 6 Press OK to return to Instrument
29. following conditions are necessary to ensure correct instrument operation and accurate results e Place the instrument firmly on an even surface Do not push any objects under the instrument e Maintain an ambient temperature of 10 to 40 C 50 to 104 F for proper instrument operation e The relative humidity should be less than 80 moisture should not condense on the instrument e Leave atleast a 15 cm 6 in clearance at the top and on all sides for air circulation to avoid overheating of electrical parts Do not operate or store the instrument in extremely dusty damp or wet locations e Keep the surface of the instrument the cell compartment and all accessories clean and dry at all times Splashes or spills on and in the instrument should be cleaned up immediately see section 7 1 on page 121 Important Note Protect the instrument from temperature extremes including heaters direct sunlight and other heat sources 13 Installation 3 3 Connection The DR 5000 has three USB interfaces as a standard feature located on the back of the instrument Figure 1 The USB Type A interfaces are used for communications with a printer USB memory stick or keyboard A USB memory stick is used to update instrument software The USB Type B interface is used for communications with a PC The optional Hach Data Trans software see section 5 2 6 3 on page 31 must be installed on the PC for this use A USB hub may be used t
30. is complete e Timer Adjusts the length of the timer sound Select Short or Long Long beeps are recommended for noisy environments 25 Standard Operations EE 5 2 5 Lamp Control 5 2 5 1 VIS Lamp and UV Lamp VIS Lamp On C Off Iv Save 2 Hours UV Switch 331 nm cl eel UY Lamp C On off Save 2 Hours 26 2 Select Long to change the number of audio signals Use the alphanumeric keypad to enter specify the number of audio signals 4 25 Note A high number of audio signals increases the duration of the tones and a small number of audio signals reduces the duration of the tones 3 Press OK to confirm The selected number of the audio signals sounds as a corresponding acoustic signal 4 Press OK to confirm The instrument will return to Instrument Setup The tungsten lamp produces light in the visible spectrum 320 to 1100 nm The deuterium lamp UV lamp available produces light in the ultraviolet spectrum 190 to 360 nm In the overlap zone from 320 to 360 nm either the deuterium lamp UV lamp or the tungsten lamp can be used for measurements The lifetime of the lamps is influenced e by on off operation and the length of time in use e Typical use is to turn the instrument on for the entire 8 10 hour shift and then off until the next day Note In general avoid on off cycles of the lamp this shortens the lamp s li
31. recordings of high resolution data take a longer time than recordings of low resolution data Selecting a larger step allows the instrument to scan faster but decreases the resolution of the collected data 95 Advanced Operations Wavelength Scan 400 nm nm Abs Min Max nm Abs Son eee Desc eee Se ss E 0 515 401 0 538 403 0 530 405 0 514 i 407 0 564 i 409 0 634 Peak Min Max Valley Peak Valley v valley Integral 323 54 Cursor Mode C Track Peak Valley Cancel OK len a Options Optic Integral 96 5 According to the selected wavelength range select the interval from the actively displayed wavelength steps In summery maximally 780 measuring steps can be accomplished during a Scan Note The maximum wavelength adjusts automatically if the difference between the maximum and minimum wavelength is not a multiple of the interval 6 Press OK to return to the scan mode Selected parameters are displayed along the graph s x axis Select view displaying table 1 Press Select View in the Options menu after a reading is taken 2 Atable with the results is displayed 3 Toreturn to the graph press Options and then View Graph Cursor mode 1 Press Cursor Mode Track in the Options menu 2 The selection for this menu item determines what data are displayed in the table Highlight Track or Peak Valley 3 Press OK to confirm 4 Press Return to retu
32. to check wavelength accuracy at 656 1 and 486 0 nm and to check bandwidth at 656 1 nm only m ee Wavelength Check 1 Press Wavelength Check in the Optical Checks menu Wavelength 486 0 nm 2 Press A to select the wavelength py Wavelength 3 Make a choice and confirm with OK 4 Press Start 486 0 nm C 656 1 nm ee Optic eS Apu Note During the warm up phase of the UV lamp the message Lamp Wavelengin Chec Warmup is displayed Wavelength 656 1 nm Lamp Warmup 107 Advanced Operations 8 tic eb bL p Wavelength Check Wavelength 656 1 nm Peak 656 0 0 636 nA Bandwidth 2 0 6 8 3 2 Noise check 9 tic Abanke p Noise Check H Wavelength 546 nm 9 tic Abanke a Noise Check Wavelength 546 nm Zeroing 24 108 5 The result is displayed 6 Press Cancel to return to Optical Checks The Noise Check test is used to test the photometric noise in the instrument This test can be used to test noise at any wavelength and at an absorbance level determined by a specific sample 1 Press Noise Check in the Optical Checks menu 2 Press A to input the wavelength 3 Input the wavelength and press OK to confirm 4 Press Zero 8 tic Abanke p Noise Check Wavelength 546 nm Reading 9 8 tics Abanke p Noise Check Wavelength 546 nm ea Average 0 6136 Abs Std Deviation 0 0001 Abs Sample 131 46 nA
33. with another active curve Two previously stored scans cannot be overlaid The reference curve is shown in gray The absorbance or transmittance value and the associated wavelength are highlighted in gray e Additionally the difference of the extinction and or transmission value between the two Scans measured Scan and reference Scan is indicated highlighted at each position of the cursor Note A black and an gray box are shown in the left corner of the display The gray box relates to the reference scan and the black one relates to the current wavelength scan Press the black or gray small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan Advanced Operations 6 7 Time course of absorbance transmittance The Time Course Mode is used to collect data in either absorbance or transmittance for a user specified length of time After the data are collected they can be displayed in either graphic or tabular format 6 7 1 Time course setup parameters 4 25 SEP 2006 Press Time Course mode in the Main Menu Press Options to configure parameters Options Abs 0 300 A Send Time amp Interval 0 Main Menu Zero Read 10 18 03 Instrument Setup Table 16 Time course setup options Option Description More For further Options Store icon To store the scan data To input the total time for data collectio
34. 0 0253 Abs 1 of 10 Advanced Operations 6 The Average and Std Deviation are calculated from 100 successive absorbance measurements The result is displayed and is to be compared with the nominal standard data given in the quality control certificate of the sample cell test filter Note Failings and passings will be defined by the user 7 Press Cancel to return to Optical Checks The Absorbance Check test is used to test the photometric accuracy and repeatability of the instrument This test can be used to test absorbance at any wavelength by a specific sample or test filter set Refer to section 6 8 3 6 on page 113 1 Press Absorbance Check in the Optical Checks menu 2 Press to input the wavelength 3 Input the wavelength and press OK to confirm 4 Remove any cuvette sample cell from the cell compartment close the cell compartment and press Zero 5 Insert sample cuvette cell or test filter into the cell compartment close the cell compartment and press Read 6 Repeat steps 4 and 5 for a total of 10 replicates to obtain final results 111 Advanced Operations MN absorbance Check 7 The result is displayed and is to be compared with the nominal standard data of the test filter If a specific sample is Wavelength 546 1 nm used the measured results must be evaluated against user defined requirements 0 0253 Abs 8 Press Cancel to return to Optical Checks Average 0 0253 Abs Std Deviatio
35. 104 6 8 Systemrcllecks 2m deae QUEE time PEG 105 6 8 1 Instrument information epu dd a cda tiep tea oda eidem eie es 105 6 8 2 Upgrade of the instrument software sssssssssssseseeeee eene eene ne nennen nennen nnn nnne 106 0 89 39 Optical AAA e tute t ere a a held qud alitem telae ite d ese sedate olas 106 60 9 3 1 Wavelength check ciclo eee er ena Due ke uti te tek acest an euge eduxit a 107 6 8 3 2 Noise check nee dcc deca 108 6 8 3 3 Stray light Check sica be be Bis e dere i rds 110 6 8 3 4 Absorbance check onsec de dd 111 6 8 3 5 Dit check oca eaemeaitetueb een eet elec Tr Ua e dades 112 6 8 3 6 Verification Kit coccion andes ente eredi eet ue Roe Eod d ete deed edd uv lan edv te dd dede 113 0 9 4 Output clieekKs iecore le eie ced es tefta deu 115 0 8 5 Lambpihistoby ir oe tere ier pnt eium TUS 115 0 0 0 Factory i i E 116 0 84 Service til Tie zi en eR nM iens 116 6 8 8 Instrument BAC A abt ette utu etes tetas 117 Section 7 Maintenance 7 emere eee eee innen neneri dne nnnnr sedere rennen nennen nnn 121 LI Cleaning QUITE MENS coria ad DIURNO TURN HEAR ERES 121 Fa Spectrophotometer n ie e Ee a ee ee ete latem ctp iunt nad scs a eas tad peered aetna dads 121 1 2 DIS Play 5 cere PEE PM eee Ein Ei 121 7 31 9 Guvettes sample cells ci RE rte t e ere dne ria testified perd 121 1 2 Eamp replacement iic isti on toii ime aimi t
36. 3 45 53 Displays tae ed n RO 21 121 Display and Sound resserre nt eerti rin 25 Drift Check vistos nnen a a 112 F A soree ien E E i E 116 Favorites nne cad Sei ERGE 22 49 80 81 delete 2 onena ee 80 81 A nn nisl el 80 recall tds boe m A 81 Filter Settings need ti eere ete eres eee ces 36 Formula e d deas 54 55 Free programming 72 73 75 78 80 H HACH Data Trans seserimi ia naiiai 31 36 Hazard Information eseeseeseeeeseen 9 l Instrument Backup ooccoccccccccnccononconcnnccnncnnnnnonns 117 118 Instrument Information ccccccccccccncncnnnnononononononos 105 Instrument Setup 22 23 43 53 88 91 95 101 Integral iiaia a aa 94 96 Interfaces 5 e loli 29 Intemet aee tdi 58 106 L Language 33 9 o n ae 19 M Main Men 3er hb e dn 22 Mainterid nce scotland ak 121 Mark one Hee I 103 Measurement Process sssss 54 55 72 Measuring sequence sse 73 Multi Wavelength 22 64 66 90 91 92 93 N Name erreia IERI eens 54 Noise Check sisse eene nnns 108 O Operating environment eee eeeeeeeeenteeeeeeeenaaes 13 Operator ID 2 2 nh iii 23 Creating E e epe E bd pat he d 23 delete s Reb caries 23 Optical Checks 106 107 108 110 111 112 113 Output Checks 2 5 mE 115 P P SSWOrd its sr Hs reise 32 activate diit Red 32 PC and Printer Reb raara 29 31 Printi
37. 4 Time Course Scan Reading 102 TIE sae 25 43 53 54 56 87 90 Touch Screen 2 1 eie c esed ters e dis 21 U Unpack the instrument ssssssss 13 Update 000 ALE 54 58 106 User Programs price bct EE tans 22 61 V Variables ennERelBexsSMS 54 56 78 Verification A 113 MOM iie n eB MM 54 55 Miew Table aiti epu 101 WwW Warranty uua edt Cre bat ra 133 Wavelength Meraner a a a a aa 87 90 Wavelength Check oooooccoccoocococococononcccccnonnncncnnannno 107 Wavelength Range sse 95 Wavelength Scan 22 38 39 40 41 94 95 98 Wavelength setting ooooocconoccccccoconocccconannncnonanno 101 Wavelength spectrum ooo eeeccecceesseeeeceeesteeeeeseenaes 94 136
38. 5134 canada hach com HACH LANGE LTD Unit 1 Chestnut Road Western Industrial Estate IRL Dublin 12 Tel 353 0 1 46 02 5 22 Fax 353 0 1 4 50 93 37 info hach lange ie www hach lange ie HACH LANGE SA Motstraat 54 B 2800 Mechelen T l 32 0 15 42 35 00 Fax 32 0 15 41 61 20 info hach lange be www hach lange be HACH LANGE S R L Via Riccione 14 1 20156 Milano Tel 39 02 39 23 14 1 Fax 39 02 39 23 14 39 info hach lange it www hach lange it HACH LANGE S R O LeSanska 2a 1176 CZ 141 00 Praha 4 Tel 420 272 12 45 45 Fax 420 272 12 45 46 info hach lange cz www hach lange cz HACH LANGE 8 Kr Sarafov str BG 1164 Sofia Tel 359 0 2 963 44 54 Fax 359 0 2 866 04 47 info hach lange bg www hach lange bg HACH LANGE E P E 27 Avlidos str GR 115 27 Athens Tel 30 210 7777038 Fax 30 210 7777976 info hach lange gr www hach lange gr Repair Service in Latin America the Caribbean the Far East Indian Subcontinent Africa Europe or the Middle East Hach Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Tel 001 970 669 3050 Fax 001 970 669 2932 intt hach com HACH LANGE GMBH H tteldorferstr 299 Top 6 A 1140 Wien Tel 43 0 1 9 12 16 92 Fax 43 0 1 9 12 16 92 99 info hach lange at www hach lange at DR LANGE NEDERLAND B V Laan van Westroijen 2a NL 4003 AZ Tiel Tel 31 0 344 63 11 30 Fax 31 0 344 63 11 50 info hach lange nl www hach lan
39. 9 001 Advanced Operations List of available absorbance formulas A4 is the absorbance at wavelength 1 A is the absorbance at wavelength 2 and so on K4 is the factor at wavelength 1 K3 is the factor at wavelength 2 and so on If a subtraction has to be completed the factors can be entered with a minus sign Wavelength 2 Press a i key and use the alphanumeric keypad to enter a wavelength Press another key and enter the next wavelength If necessary repeat until all the wavelengths for the formula have been entered The wavelengths must be in the range from 190 1100 nm Press OK Concentration Factor Kx Multiplication factor for converting absorbance values into concentration values Press a factor key and use the alphanumeric keypad to enter a factor If the formula includes more than one factor press another factor key and enter another factor and repeat until all the factors have been entered Press OK When all the relevant data have been entered press Next Note Up to five digits can be entered including a maximum of 4 decimal places to the right of the decimal Concentration resolution number of decimal places Select the required number of decimal places from the displayed list and press Next 65 Advanced Operations Chemical Form Chemical form its NON Enter the chemical formula used in the display to represent the analysis parameter Use the alphanumeric keypad to enter the
40. Always wait about 20 seconds before turning the instrument on again otherwise the electronic and mechanical systems will be damaged The spectrophotometer software includes several language options The first time the instrument is turned on the language selection screen will appear 1 Select the desired language 2 Press OK to confirm the language selection The self check will start automatically Changing the language setting The instrument functions in the selected language until the option is changed 1 While turning the instrument on touch the screen at any point until the list for selecting a language appears about 30 seconds 2 Select the required language 3 Press OK to confirm The test program subsequently starts automatically Each time the instrument is powered up a series of diagnostic tests are performed automatically to ensure operation of major system components This procedure which takes approximately two minutes checks the system lamp filter adjustment wavelength calibration and voltage Each test which functions correctly is confirmed with a check mark The Main Menu is displayed when power up diagnostics are completed Note Further error messages during self check see Section 8 on page 127 19 Start Up 20 Section 5 Standard Operations 5 1 Overview 5 1 1 Tips for the use of the touch screen The entire screen is touch activated To make a selection press the scr
41. Cell 1x1 cm Quartz 450 uL Glass center height 10 mm Pour Thru Cell 5x1 cm Quartz 370 uL Glass center height 10 mm Pour Thru Cell 1 Square 1 path length Adapter for 100 mm Sample Cell Dust Cover Cat No LZV485 LZV513 LZV479 A23618 A23620 LZV582 LZV566 LZY274 LZV659 A23800 LZV580 LZV780 LZV569 2946900 LZV567 A23778 A23792 A23772 A23766 LZV537 LZP269 LZP331 2095100 4822800 2629250 2624450 2629201 2624401 4864302 2495402 2665902 2410212 A24209 LZV510 LZV649 5913700 LZY421 LZV728 129 Replacement Parts 130 Section 10 Contact Information HACH Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Tel 800 227 HACH 800 227 4224 U S A only Fax 970 669 2932 orders hach com www hach com HACH LANGE GMBH Willst tterstrae 11 D 40549 D sseldorf Tel 49 0 2 11 52 88 320 Fax 49 0 2 11 52 88 210 info hach lange de www hach lange de DR BRUNO LANGE AG Juchstrasse 1 CH 8604 Hegnau Tel 41 0 44 9 45 66 10 Fax 41 0 44 9 45 66 76 info hach lange ch www hach lange ch HACH LANGE APS Akandevej 21 DK 2700 Br nsh j Tel 45 36 77 29 11 Fax 45 36 77 49 11 info hach lange dk www hach lange dk HACH LANGE LDA Av do Forte n 8 Frac o M P 2790 072 Carnaxide Tel 351 214 253 420 Fax 351 214 253 429 info hach lange pt www hach lange pt HACH LANGE KFT Hegyalja t 7 13 H 1016 Budapest Tel 36 06 1 225 7783 Fax 36
42. Clean Cuvette The cuvette is soiled or there are undissolved particles in the cuvette Clean the cuvette allow the particles to settle Error Please check the UV Lamp The lamp output is too low Check the lamp and replace it if necessary Error Selfcheck stopped Please check the lamp Please close the lid Error xx Self Check Test stops while starting the instrument Check the lamp and replace if necessary Close the lid Press Start Again Error Selfcheck stopped Please remove the cuvette Please close the lid Error Sipper Self Check Test stops while starting the instrument Kink in tube Peristaltic pump failure Remove the cuvette sample cell from the cell compartment Press OK Check the tube connections Error Selfcheck stopped Hardware error Error x Electronic defect Contact the manufacturer or a sales representative and indicate the error number Negative result The calculated result is negative Check the concentration of the sample No evaluation Error in the test database user database Check the programming Contact the manufacturer or a sales representative Over measuring range The measured absorbance is above the calibration range of the test Dilute the sample and repeat the measurement Under measuring range The measured absorbance is below the calibration range of the test If possible select a test with
43. Here are a few examples of entries e Programming of user created procedures The analysis procedure must be developed first before it can be programmed The user must define or determine the program sequences calculation formulas measurement wavelengths factors measuring range limits etc Modified tests e Assignment of user programs to the favorites menu for frequently used tests e Creation of a specific selection of methods and tests Press User Programs in the Main Menu and then Program Options The Program Options menu contains several input and editing options Table 7 Add to Der Favorites Edit Delete Cancel Program Options Table 7 Program options user programs Options Description 1 New Select New to program a new user program Note The first time Program Options is selected only the New option is available The other options remain inactive gray until the first program has been created 1 Add to favorites Select Add to favorites to add an existing user program to the list of frequently used programs 1 Edit Select Edit to modify an existing program 1 Delete Select Delete to remove a program from the list of user programs The program will be simultaneously deleted from the Favorites list 6 1 1 Program a user method All input steps and their significance and options are explained in the following sections 1 Select New in the Pr
44. R aua 42 5 4 3 Use of program tlmers terca e E LO Poe e HN Ua Fr e P P D bad ite 44 5 4 4 Set the reading mode oe se a 44 5 4 4 1 Take single wavelength measurements single reading sse 45 5 4 4 2 Take single wavelength measurements continuous readings seeee 45 5 4 5 Set the dilutionifactor ze tute Ub a ro ie Pre tbe re C Br de E lad Leste Pipe bici 45 5 4 6 Run a standard adjust A ec m Pd eR 46 5 4 7 Setthe chemical TOM ssns reeda deariden iiaii ete edu Hoe oed ze eri 47 5 4 7 1 Change of the default setting of the chemical form sssss me 47 9 4 8 Runa Tag nae E 48 5 49 Analysis of samples seem cre etie AA A A as 49 5 4 10 Add stored programs to the favorite programs list oooonnnccccnnnnccccconna cacon naonnccnn nano n cc ncn ano en 49 5 5 Barcode Programs calma P tu ee pe iid high ete d bet Un D HE E eee 50 5 5 1 Complete a barcode 13 mm test vial ooooonoccnninncnncccnncnncccccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nro eren nnn nn nennen 51 5 52 Select the measuririg Tange c iio EL see Pla e a t i gta Ltd Lo edu n Aaa d artnet 51 5 5 3 Select the chemical evaluation form sssssssssessseneeee eene nenne nennen 51 5 5 3 1 Change of the default setting of the chemical form sssssss me 52 5 5 4 Basic test specific and sample specific data settings ssssssssssssee eee 52 5 5 5 Samlple blarik 32 NN 53 5 5 6 Update edit bar
45. Verification Stray Light Check KV450 3 PASS Nominal Value gt 2 8 Abs Photometrical Accuracy NG9 1 PASS Nominal Value 1 493 Abs Actual Value 1 493 Abs concer A Y 6 8 4 Output checks 6 8 5 Lamp history n UT n Lamp History un VISIBLE Hours 90 4 Reset VIS Cycles 72 P uv Hours 0 1 Reset UV Cycles 3 Triggers 7 O eee Advanced Operations 8 Insert the different filter in the given order one after the other with the filter glass facing the front of the instrument Press Next after inserting a filter and closing the cell compartment After the last measurement the results are displayed 9 Press PC amp Printer icon to send the data to a USB memory stick PC or to a printer The files will be stored automatically as CSV file Comma Separated Value The file name will be formatted as Verification csv If a printer is connected a test printing of the current screen will be printed The Lamp History menu provides the following information The amount of time that the lamp has been on Hours The number of times that the lamp has been turned on Cycles The number of times that the instrument tried to turn the UV lamp on triggers After a lamp is replaced and the Lamp History is reset the display of the total operating time is reset to O 1 Press Lamp History in the System Checks menu 2 Press Reset VIS and the Visible Lamp will be reset 3 Press Reset UV a
46. a ii HA ede A Teque ao pois Te a eee 31 5 2 0 3 HACHA Data MES inte aet f e ls te ea ea tee ee a ERE Az 31 5 2 Password or da HDI E 32 5 2 71 Password de activati n ion ete eite per octal 33 5 3 Store recall send and delete data saaien ga aara a a aaa a aaa iaa a Td a aadi 35 DSA THe Data LOG eet petii euet a ale EU I eo brine a EAE 35 5 3 1 1 Auto manual data Stage mins 35 5 3 1 2 Recall stored data from the data log oononiccinnnnnnocicnnncccccnnncnnnnonnnnnnnna nana n nano nennen nnns 35 5 3 1 3 Send data from the data log ssssssssssssssee eene nnnm en nennen nnns 36 5 3 1 4 Delete stored data from the data log ssssssssssseeee enne nennen nnns 37 5 3 2 Store recall send and delete data from wavelength scan and time course ssssesssssss 38 5 3 2 1 Data storage from wavelength scan or time course sssss eee 38 5 3 2 2 Recall stored data from wavelength scan or time course ssssssse e 38 5 3 2 3 Send data from wavelength scan or time course sssssssssssse ene 39 5 3 2 4 Delete stored data from wavelength scan or time course sssss e 41 Table of Contents 5 4 Stored Programs naci ad dd d dr Pe d dus 42 5 4 1 Select a saved test method enter user specific basic data oooocononinoncncncnononononococncnnnnnnnnnnononanncnonanonos 42 5 4 2 Stored program options vissna ae A d da da Dna needed sae saeua M
47. a lower measurement range or use a cuvette with a longer path length 127 Troubleshooting 128 Section9 Replacement Parts Description Sipper Module DR 5000 Peltier temperature controlled Module DR 5000 Pour Thru Module DR 5000 Multi Cell Holder DR 5000 Carousel Holder Sample changer USB Keyboard keyboard layout US USB Barcode Scanner handset scanner for entering sample IDs Hach Data Trans PC software for data transfer Brewery Analysis Software Sipper Tubing Inlet and Waste Sipper Tubing PHARMED quality Sipper Tubing comes standard with Sipper Module Pour Thru Tube Kit USB Memory Stick USB Interface Cable 1 m Tungsten VIS Lamp Deuterium UV Lamp Fuse Filter Pad Certified test filter set for self checks Verification Kit 6 precision glass filters with target values Semi Micro Cell 50 mm Glass Macro Cell 20 mm Glass Sample Cell 1 cm Square Glass 10 mm path length matched pair Sample Cell 1 cm Square Quartz 10 mm path length matched pair Sample Cell 50 mm Rectangular Glass Sample Cell 50 mm Rectangular Quartz Sample Cell 100 mm Rectangular Glass Sample Cell 100 mm Rectangular Quartz Sample Cell 1 Round Plastic 1 cm path length 10 mL w cap Sample Cell 1 Square Glass 10 mL matched sets Sample Cell 1 Square Glass 10 amp 25 mL matched sets Sample Cell 1 Square Plastic Pour Thru Cell 1x1 cm Quartz 160 uL Glass center height 10 mm Pour Thru
48. able Rags Keds KAEKA eK AS Ra A KoA Ka AGES Kap hal Key Ac K4A4 K2A2 K3A3 K41A4 K2A5 K3A3 K4A4 A 4 refers to the absorbance at wavelength 1 A 2 refers to the absorbance at wavelength 2 etc K refers to the coefficient at wavelength 1 K refers to the coefficient at wavelength 2 etc Coefficients can be set negative where subtraction is required 91 Advanced Operations Optic F Concentration Factor On C off Factor Unit somo man Cancel OK en 92 1 To change a wavelength press one of the 1x keys Enter the desired wavelength coefficient into the numeric keypad Press OK to confirm To change a coefficient press one of the Ky keys Enter the desired coefficient into the numeric keypad Press OK to confirm Note The instrument allows entry of up to 5 significant digits with a maximum of 4 significant digits after the decimal point Concentration factor Press Concentration Factor Off in the Options menu Press On to highlight this feature Press the Factor key to enter the factor by which absorbance readings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit 3 Press OK to confirm Concentration resolution 1 Press Concentration Resolution in the Options menu 2 Select the resolution and press OK to confirm Advanced Operations 6
49. ains the following application modes Stored Programs User Programs Favorite Programs Single Wavelength Mode Multi Wavelength Mode Wavelength Scanning Mode and Time Course Mode The DR 5000 is used for testing in visible and ultraviolet wavelengths A gas filled tungsten lamp produces light in the visible spectrum 320 to 1100 nm and a deuterium lamp available produces light in the ultraviolet spectrum 190 to 360 nm The DR 5000 Spectrophotometer provides digital readouts in direct concentration units absorbance or percent transmittance When a user generated or programmed method is selected the on screen menus and prompts direct the user through the test This menu system also can be used to generate reports statistical evaluations of generated calibration curves and to report instrument diagnostic checks 11 General Information 12 Section3 Installation 3 1 Unpack the instrument 3 2 Operating environment WARNING Electrical and Fire Hazards Use only the provided power supply Only qualified personnel should conduct the tasks described in this section of the manual The DR 5000 Spectrophotometer comes packaged with the following items e DR 5000 Spectrophotometer e Mains power cable e Multi Cell Holder e DR 5000 User Manual e CD ROM containing the HACH and HACH LANGE procedures Note If any of these items are missing or damaged contact the manufacturer or a sales representative immediatly The
50. ally n ute ee teet cease t Misi duri re de 18 SECTION 4 Scream 19 4 1 Power the instrument on and off cccccccccecee cece eeeeeeee nee aeee eee cnn nn nana aa eaaeeaeceeeeeeeeeeeesasecaaaeaeeeeeeeeeeseeeeneseaeees 19 AD LANGUAGES Selecion cidcid m 19 4 9 Self Gheck RA 19 Section 5 Standard Operations ooooooccoccccoccccccccccccccccnnnnonno cnn nncnnn nn e aa aeiiae a e aae Aa aa dE dE aa 21 NBe a M a O A A e att im eds cal 21 5 1 1 Tips for the use of the touch screen oooccccccccononooocnncnnnnnncnnnnnnnnnnnn cnn nnnnnnnnnnnn nn nena enn ener er nnne nnn nnne rennes 21 5 1 2 Use of the alphanumeric keypad sss eere nn nene n nnne ns 21 5 1 3 Main Menu it dL e de ioo digest Leges eee pei to de ae Ee dede Ue anil Aad eee 22 5 2 Instrument Setup mode un eet ieu tete teli diede bt emend 23 Dz Operator Di ir tui ael rst cii Ein 23 RAPAVTuOaba EST 24 5 2 3 Date and tlme nl ta tee aaa 25 5 2 4 Display and sound preferences ccecceceeeceececcece eee eeeee cesta ee eaeaaeeeceeeeeeeeeeeeesaeeaaaaeaaeaeeeeeeeeeeeseeenenenaeess 25 ZO Lamp CONTO Mer H nea rica ie ET 26 5 2 5 1 VIS Lamp and UV L amp s i ari aaa a id ida 26 5 2 5 2 Effect caused by the lamp settings on the Measuring Mode sees 27 52 6 PG and printer coro dir Re decor es tet iade pto pepe D see uode dub ee deb aded Sete 29 5 2 0 1 Printer Setup dicta e ER e abe sem ur aeter 29 5 2 6 2 Print dat
51. an one or more samples 1 Press Wavelength Scan in the Main Menu 2 Insert the blank cuvette cell into the cuvette sample cell holder 97 Advanced Operations Wavelength Scan 400 Reading Main Menu Cancel Wavelength Scan 0 300 400 600 Integral 323 54 a Options 3 Press Zero Zeroing appears below the graph as the baseline scan begins 4 Insert the prepared sample cuvette cell into the cuvette sample cell holder 5 Press Read Below the graph Reading appears and a graph of the absorbance or transmittance values at the scanned wavelengths is displayed continuously The Wavelength Scan is complete if e the graph is shown full size e the scaling of the x axis fits automatically e the Cursor functions in the vertical navigation bar are highlighted 6 6 2 1 Navigation of the wavelength scan graph or a wavelength scan analysis Table 15 Navigating the wavelength scan Cursor Function Zoom Function Description Curve Icon Choice of Cursor Mode Peak Valley cursor moves between minimum maximum absorbance Choice of Cursor Mode values or Cursor Mode Tracking cursor moves over each data point of the scan The arrow keys are used right left to move the cursor depending on the selected mode to the next data point The data of the data point wavelength absorbance or transmittance value are Arrow keys highlighted on the x and y axes Not
52. and can have a positive or a negative sign 69 Advanced Operations 6 1 1 4 Store a user program User Program 950 Name User Program Chemical Form 1 NOsM Cancel The input of the basic data is complete An overview of the variable program data is displayed 4 To enter more specifications or change existing ones highlight the appropriate line and press Edit 5 Select Store to save the user program 6 Press the PC amp Printer icon to send the program data to a printer or to a USB memory stick connect the USB memory stick to the USB interface first Note The test data will be formatted in the preinstalled PrgData folder as a csv and as a Ist file 7 To transfer the program data from one instrument to another create a new folder on the USB memory stick named dbhl and copy the Ist files into this folder see section 6 8 2 on page 106 6 1 1 5 Additional user defined parameters and functions nen Upper Limit 70 In addition to the previously defined basic data additional parameters and functions can be defined for user programs e measuring range upper and lower limits e timer functions e chemical forms Upper and lower limits of the measuring range It is possible to enter a maximum upper and minimum lower measurement value An error message is displayed if a reading is above the upper limit or below the lower limit 1 Highlight the appropriate line in the overview of th
53. asuring data Operator ID Sample ID passwords and all adjustable data on a USB stick Syste Ahani Instrument Backup 1 Press Instrument Backup in the System Checks menu 2 Connect the USB memory stick section 3 3 on page 14 3 Press Store to start a Backup Note If the USB stick is not connected the message Please insert USB Memory is displayed Connect a USB stick in order to store the data Press OK to confirm and press Store again SEEEEEEEEEEEES Store Please insert Rc USB Memory 117 Advanced Operations systr Abanke Store Data already exist Overwrite COO Store Instrument Backup is m stored to USB Memory de OK Systr Abanke Instrument Backup We HI E E 4 Cancel 118 Note If the Backup was already stored before the message Data already exists Overwrite is displayed Press OK to overwrite the data If the file was stored the message Instrument Backup is stored to USB stick will be displayed 4 Press OK to return to the System Checks menu Restore backup data Important Note All current data will be overwritten when restoring the Backup file 1 Press Instrument Backup in the System Checks menu 2 Connect the USB memory stick containing the Backup section 3 3 on page 14 3 Press Restore to pass back the data Advanced Operations as Note If the USB stick is not connected the message Please insert USB
54. ay The data overview is then displayed 6 1 2 3 Enter the calibration formula evaluation formula User Program 951 Cancel Formula The calibration formula evaluation formula defines the calculation and display of intermediate and final results The previously defined elements of the measuring sequence are the basis for calculating the concentrations Enter calibration formula C1 1 Highlight the Formula line in the data overview and press Edit 2 Highlight the line C1 Off and press Edit 3 Select C1 Off again and press Edit The display changes to C1 On 4 Highlight the next line C1 to define the formula and press Edit 75 Advanced Operations _ yz Edit Formula C1 Select Abs Conc Variables New re Number x gt lt bs concer o Refer to Table 11 for detailed information on the Edit formula keys Note The evaluation formula is built up successively in the display in accordance with the input Note The arrow key deletes the most recently entered element of the formula Table 11 Edit formula key descriptions Screen Key Description Abs Variables Press the Abs Variables key to select from the displayed list the required element of the defined Abs Variables measuring sequence and therefore the corresponding measurement wavelength so that this can be taken into account in the formula
55. ayed Go to section 6 1 1 4 on page 70 67 Advanced Operations 68 Calibration C Enter Values Read Standards C Enter Formula Calibration by reading standards Press Read Standards and press Next To enter the standard concentrations in the displayed table press the symbol Use the alphanumeric keypad to enter the standard concentration Press OK Press the symbol again see arrow and enter the next standard concentration Repeat this sequence until all standard conentrations maximum of 24 solutions have been entered Highlight the line with the appropriate concentration and insert the cuvette cell with the corresponding standard solution Insert the zero solution into the cell compartment Press Zero Insert the first standard solution into the cell compartment Press Read Insert the second standard solution into the cell compartment Press Read Repeat this sequence until all the standard solutions have been measured maximum of 24 solutions The entered and measured data are displayed in the table Note To delete a standard concentration highlight the appropriate line and press the Delete icon The timer icon shown in the display helps to ensure when necessary that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influe
56. based on method selection Scanning speed 900 nm min 1 nm steps Spectral bandwidth 2nm Photometric range 3 0 Abs in Wavelength Range 200 900 nm Photometric accuracy 5 mAbs at 0 0 0 5 Abs 1 at 0 50 2 0 Abs Photometric linearity Stray light lt 0 5 2 Abs lt 1 at gt 2 Abs with neutral glass at 546 nm Ki solution at 220 nm gt 3 3 Abs lt 0 05 Data storage 1000 measured values result date time sample ID user ID User programs 50 Physical and environmental specifications Width 450 mm 17 7 in Height 200 mm 7 9 in Depth 500 mm 19 7 in Weight 15 5 kg 34 2 Ibs Operating requirements 10 40 C 50 104 F max 80 relative humidity non condensing Storage requirements 25 60 C 13 140 F max 80 relative humidity non condensing Additional technical data Mains connection 100 240 V 50 60 Hz automatic changeover Interfaces Use only shielded cable with max length of 3 m 1 x USB type B 2 x USB type A Water resistant to Enclosure IP 31 Safety class Safety class Specifications Section 2 General Information 2 1 Safety information Please read this entire manual before unpacking setting up or operating this equipment Pay attention to all danger warning and caution statements Failure to do so could result in serious injury to the operator or damage to the equipme
57. by pressing it or press Select by Number to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad to enter the test number program number and press OK to confirm Press Program Options Press Add to Favorites Edit or Delete and press OK to confirm Note If the stored program is deleted in User Programs it will also be deleted in Favorites Programs 79 Advanced Operations 6 1 4 1 Add to Favorites Program 950 User Program Add to Favorites Cancel oK p 6 1 4 2 Edit User Program 950 Name User Program Cancel 6 1 4 3 Delete Program 950 User Program Delete Program Cancel 80 1 Press Add to Favorites and press OK to confirm The program is added to the Favorites 1 Press Edit and press OK to confirm An overview of the specifications of the programmed test is displayed More information about the input options is provided in the section 6 1 2 on page 72 1 Press Delete and press OK to confirm The program is deleted from the list of User Programs Note If the stored program is deleted in User Programs it will also be deleted in Favorites Programs Advanced Operations 6 2 Favorite Programs 6 2 1 Recall a favorite program Favorite Programs 906 NHa 950 User Program Remove Program 6 2 2 Delete a favorite program Program 906 NH4 Remove from Favorites
58. chemical form and press Next to enter Calibration settings 00 JKL MNO Par des EXE 6 1 1 3 Calibration settings for single and multi wavelength mode A method is calibrated by determining the absorbance values of several standard solutions of known concentration There are three ways to create and store a calibration curve Instructions for each method follows Table 9 Table 9 Calibration settings Mode Enter values Descriptions A calibration table is created by entering the concentration values and the absorbance values of the analyte solution The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph page 66 Read Standards A calibration table is created by entering the concentration values of the standard solutions and then measuring the absorbance of the analyte solutions The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph page 68 Enter Formula If the calibration curve can be determined from the mathematical relationship between concentration and absorbance by linear regression etc the corresponding formula can be selected linear 2nd or 3rd order polynomial from a list and the appropriate factors can be entered page 69 nen Calibration by entering calibration values Enter concentration absorbance Calibration 1 Select the Enter Values and
59. code tests sssssssssssssssssssssssssssssseenere tentent rnnt rei tn nennen nennen nnns 54 5 5 6 1 Manual update of a barcode test sssssssssssssssssssssssseeere eere rrerrne nnne 54 5 5 6 2 Update an existing barcode test ssssssssssssssseeee eene nennen enne nnns 57 5 5 6 3 Program a newtest easiest eee seen e ee iin e ne Dl s ad an dor e adi 58 5 5 7 Upgrade of the instrument software ooooonccconncoccccccononnccnccononnnccnn nano nc ncnn nana nc nc nano nn nennen nennen enne 58 Section 6 Advanced Operations sss eene emenen ennemi nnn nene nennen nenr nennen nnn 61 6 1 User Programs 1 onere PEL ed Eco gus ee nd onde Loo OR zo o oo luct Lo de edges 61 6 11 Program a User Method Vecina o pap cda enit ed 61 6 1 1 1 Single wavelength settings oononniininnnn nnnnncnnnnnnnraccnn crec 63 6 1 1 2 Multi wavelength settings rnini peiie iea nono nono eee EATA en nenne enne nnns 64 6 1 1 3 Calibration settings for single and multi wavelength mode s 66 0 1 1 4 Store a user program zennad me bre eee i e e pe te t d e e e t RU 70 6 1 1 5 Additional user defined parameters and functions sss 70 6 1 2 Free programming program type iriiri anar eer entente nnns aite tnnt andinas 72 6 1 2 1 Measurement process coccccccoconcononcnncconnnnncnnnnn anno ttt nn nn nn nennen nemen nn sn nn nn nenne tene nrten nsns nennen 72 6 1 2 2 Enter a new ele
60. concentration of the analyte in the sample and their precision correspondence of the measurement results obtained from several samples containing the same concentration of the test analyte can be determined or improved using the standard addition method This method also referred to as spiking serves to identify sample specific interference factors e g substances in the sample that falsify the analysis sample matrix effect a defective measuring instrument or contaminated reagents Method A defined amount concentration of a standard solution of the test substance is added to the sample The detection rate should be close to 100 Measured value after a standard addition Detection rate Expected value after a standard addition Detection rate Conclusion Probability that the measurement results are correct 100 Suc is high Assumption The analysis was falsified by substances in the sample sample matrix effect lt 100 Test to determine whether a sample matrix effect o is present Use distilled water instead of the sample Add standard solution as described in the procedure Detection rate Conclusion lons in the sample are interfering with the analysis causing false results to be obtained 100 No interfering ions 100 consider other interference factors Measures to identify other interference factors Checklist 1 Check if the procedure is completed correctly a Ar
61. cursor depending on the selected mode to the next data point The data of the data point wavelength absorbance or transmittance value are Arrow keys highlighted on the x and y axes Note Press any point on the curve to display the associated data This function is used to magnify the section of the curve in the vicinity of the cursor The original Zoom Icon curve size can be restored by pressing the zoom icon again 104 Advanced Operations 6 8 System checks Main Menu 1 Press System Checks in the Main Menu Instrument Setup System Checks The System Checks menu contains instrument information and Instrument Instrument various performance tests Information Update Optical checks output checks Lamp wistory Factory Service S ice Ti Instrument ervice lime Backup est 6 8 1 Instrument information Bystr erm m s y Instrument Information 1 Press Instrument Information in the System Checks menu DR 5000 S N 1130961 2 The model serial number and software version are displayed Instrument Version 1 06b 33 2 6 Program Versions HACH 06 HACH LANGE 53F ox 105 Advanced Operations 6 8 2 Upgrade of the instrument software Systr Abanke Instrument Update Please insert an Instrument Update Module into a USB Port E E E E de connect to a computer that is running the update program 8 E 6 8 3 Optical checks Optical Checks Wavel
62. data are collected the following manipulations can be done on the graphic data Time Course VIS 400nm The Time Course Program is complete if e the sound is turned on the instrument beeps when the readings are done e the graph is shown fullsize e the scaling of the x axis occurs automatically e the Cursor functions in the vertical navigation bar are highlighted Options 103 Advanced Operations 6 7 3 1 Navigation of a time scan or a time scan analysis After a time scan has been completed the time and the absorbance transmittance data are displayed as a curve Where the cursor is positioned on the curve the elapsed time up to this point and the corresponding absorbance are highlighted Table 17 Navigating the time scan Cursor Function Zoom Function Description Curve Icon Choice of Cursor Mode Delta mode A second cursor is highlighted The position of the fixed cursor was previously defined in Cursor Mode Single Use the active cursor to select any point on the measurement curve The difference to the fixed cursor is shown on the curve The delta values are correspondingly highlighted and displayed on the x and y axes The gradient of the curve and the correlation coefficient r2 between the cursor points in the Delta mode are shown under the curve Cursor Mode Single The cursor moves to each selected measurement point of the scan The arrow keys right left are used to move the
63. datal aan 31 Printer Setup uni ep eee ib Ea 29 Printinig data iio ede 31 Program dad ns 61 Programm overview sseeeennnne 22 R Reading Mode sssssss 43 44 88 89 Reagent Blank ooooconnonccccococccocccocononoccccncnonononos 43 48 Recall Data 22 38 43 53 88 91 95 101 Reference Scan iieri eei iiei 94 99 S Safety Information ooonncccnnnnnncccccnnnocconnnononannccnncnnnno 9 Sample blank sse 53 Sample ID coito tii ii itd 24 create at it tos 24 delete e ene 24 Save as User Program 43 53 88 90 Scale amp Units 95 97 101 102 Security LISE aiii Hen e Le HE Ded 32 Select VieW 4 sm tete ete a o Hem d 96 Send Data 36 43 53 94 101 Service Time aieiaa ee eee 116 Single Reading ssseeeeee 43 Single Wavelength 22 43 45 63 66 87 88 89 SOMWANC eai ed eset ta deii 58 106 Standard Addition ooooconononinononononononnnncncnonononos 43 82 Standard Adjust 43 46 Stored Data si Ne Wb tuto 35 Stored Programs 22 42 43 45 46 47 48 49 Send Data vic 43 Stray Light Check oriniai ia 110 System Check 0 ccccccceeeceetesesseeeeeecersesneneeseereenenenees 116 System Checks 22 105 107 108 110 111 112 113 p RA AE Le 115 116 117 135 T Time amp Interval ssnin aea 101 102 Time Course 22 38 39 40 41 101 103 10
64. defined automatically and the OK key is inactivated Scale amp units 1 Press Scale amp Units in the Options menu 2 Highlight Abs or T as the required units 3 Highlight Auto or Manual scaling on the graph s y axis Note If manual scaling is selected use the alphanumeric keypad to set the limits Y min and Ymax The graph is adjusted to display only the values in the selected range If automatic scaling is selected the instrument sets the limits automatically so that the total range can be displayed 4 Press OK to confirm 5 Press Return to return to the scan mode After the parameters have been selected the instrument must be blanked then the sample can be analyzed 1 Insert the blank cuvette cell into the cuvette sample cell holder Press Zero The blank reading is shown on the display 2 Insert the sample cuvette cell into the cuvette sample cell holder Press Read Start collecting time course kinetic data Note During the measurement the Zero and Read keys change to Mark and Stop Advanced Operations Time Course VIS 400 nm e Select Mark to mark the next data point collected This mark is not used by the instrument but is available for the user and may indicate a significant event such as the addition of a sample or other reagent The mark is also shown in the table e Select Stop to stop taking sample readings Next Reading 00 09 6 7 3 Analysis of time course data After the
65. e Press any point on the curve to display the associated data This function is used to magnify the section of the curve in the vicinity of the cursor The original Zoom Icon curve size can be restored by pressing the zoom icon again 98 6 6 3 Work with reference scans Select Reference Scan 01 SEP 06 11 05 34 Scan 1 400 600 nm 001 nm 06 09 44 43 400 600 nm 001 nm 11 15 54 400 600 nm 001 nm 13 13 32 400 600 nm 001 nm 14 06 58 400 600 nm 001 nm Reference Highlight Highlight Reference Data Reference Scan Difference 0 758 Exit Zero Options Advanced Operations There are two options to work with Reference Scan Option 1 1 Press Reference Off in the Options menu to select another scan to display on the same screen with the current scan Highlight the required scan number and press Highlight Reference Note After selecting a reference scan the Reference Off key in the Options menu turns into Reference On Note Only scans that have the same wavelength range and step can be displayed using the overlay option A reference curve can only be displayed with another active curve Two previously stored scans cannot be overlaid The reference curve is shown in gray The absorbance or transmittance value and the associated wavelength are highlighted in gray Note A black and an gray box are shown in the left corner of the display The gray box relates to th
66. e length of the timed interval and press OK to start the timer The timer will beep when the timed interval ends 1 To highlight the required mode press Reading Mode 2 Select the required mode then press OK then Return to return to the result display Standard Operations 5 4 4 1 Take single wavelength measurements single reading 10 Aluminum Alumin MEROS 522 nm 0 108 aaa 13 OCT 2006 11 45 55 e A Options Insert the blank cuvette into the cuvette sample cell holder Press Zero Note The Read key is only active after the zero measurement has been carried out Insert the sample cuvette into the cuvette sample cell holder Press Read Note For data storage see section 5 3 2 on page 38 5 4 4 2 Take single wavelength measurements continuous readings 10 Aluminum Alumin 0 057 ES Abs Trans aaa 13 OCT 2006 11 46 13 5 4 5 Set the dilution factor Dilution Factor d Cancel OK Insert the blank cuvette into the cuvette sample cell holder Press Zero Note In the reading mode Continuous only the Zero key is shown to start the reading The reading sequence is started automatically Insert the sample cuvette into the cuvette sample cell holder Press Options and then the Store icon to store the displayed data in the Data Log Note For data storage see section 5 3 2 on page 38 The Dilution Factor function is used to calculate the original conce
67. e program data and press Edit 2 Set On and press the 0 000 key to enter the measuring range limit Confirm the entry by pressing OK Advanced Operations nen Timers Timer 1 Timer 2 Timer 3 Timer 4 z mem This function can define time intervals for up to four timers Timer DENS designations such as Shake Wait and Swirl can be assigned 1 Highlight the appropriate line in the overview of the program _ Timer 00 00 data and press Edit Timer 00 00 2 The timers are activated or deactivated with the check boxes in the left part of the display In the next column a selection can Cancel E be made from a list of names that designate the corresponding work step In the third column the times for each timer are entered in mm ss nen Chemical Forms Chemical form 2 chemical form 3 chemical form 4 a If a Chemical Form 1 has been defined up to three additional Nos TR alternative forms can be entered here 1 0000 3 A 0000 1 Highlight the appropriate line in the overview of the program 1 0000 data and press Edit 1 0000 2 The chemical forms are activated or deactivated with the check a boxes in the left part of the display Cancel ES 3 Press the left key to enter another chemical form with the alphanumeric keypad and press OK to confirm Press the right key to enter the conversion factor to calculate the concentration of the additional chemical form from the concentratio
68. e reference scan and the black one relates to the current wavelength scan To complete the wavelength scan reading see section 6 6 2 on page 97 e The newly plotted wavelength scan curves are shown in black The absorbance or transmittance value and the associated wavelength are highlighted in black e In addition the display shows the difference between the wavelength scan curve and the reference curve against the wavelength Press the black or gray small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan 99 Advanced Operations Difference 0 758 100 Exit Zero Options Option 2 Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Insert the sample cuvette cell into the cuvette sample cell holder Press Read e The newly plotted wavelength scan curves are shown in black The absorbance or transmittance value and the associated wavelength are highlighted in black Press Options and then Reference Off in the Options menu to select another scan to display on the same screen with the current scan Highlight the required scan number and press Highlight Reference Note After selecting a reference scan the Reference Off key in the Options menu turns into Reference On Note Only scans that have the same wavelength range and step can be displayed using the overlay option A reference curve can only be displayed
69. e returned without authorization from Hach Company Limitations This warranty does not cover Damage caused by acts of God natural disaster labor unrest acts of war declared or undeclared terrorism civil strife or acts of any governmental jurisdiction Damage caused by misuse neglect accident or improper application or installation Damage caused by any repair or attempted repair not authorized by Hach Company Any product not used in accordance with the instructions furnished by Hach Company Freight charges to return merchandise to Hach Company Freight charges on expedited or express shipment of warranted parts or product Travel fees associated with on site warranty repair This warranty contains the sole express warranty made by Hach Company in connection with its products All implied warranties including without limitation the warranties of merchantability and fitness for a particular purpose are expressly disclaimed Some states within the United States do not allow the disclaimer of implied warranties and if this is true in your state the above limitation may not apply to you This warranty gives you specific rights and you may also have other rights that vary from state to state This warranty constitutes the final complete and exclusive statement of warranty terms and no person is authorized to make any other warranties or representations on behalf of Hach Company Limitation of Remedies The remedies of
70. e the reagents added in the correct order b Is enough time allowed for color development c ls the correct glassware in use d Is the glassware clean Advanced Operations e Does the test require the sample to be at a certain temperature f Was the pH of the sample in the correct range g Is the pipette volume correct 2 Check the used reagents by repeating the standard addition procedure with freshly prepared reagents Detection rate Conclusion The originally used reagents were defective Check the standard solution Repeat the standard addition procedure with a freshly prepared standard solution 100 Detection rate Conclusion The originally used 100 standard solution was defective If none of these measures resolves the problem please contact the manufacturer or a sales representative 6 3 1 Complete a standard addition Carry out the standard addition in accordance with the corresponding procedure There are two different methods Peak volume Standard addition Defined volumes of a standard solution are added step by step to an already analyzed sample The sample is measured again after each addition Sample volume A defined volume of a standard solution of known concentration is added to the volume of sample specified in the procedure and the sample is measured after each addition In most cases three different standard solutions are prepared and the proced
71. ed to any point on the curve for the purpose of reading off the absorbance or transmittance value and the wavelength The data associated with each data point can also be shown as a table 6 6 1 Set up the wavelength scan Press Wavelength Scan in the Main Menu Press Options for Parameter Setup Options Cursor Mode cH Send Track Data reo Scale amp Units n Reference off 400 25 SEP 2006 A nm 11 10 07 Instrument Return Setup Table 14 Wavelength scan setup options Option Description More For further Options Store icon To store the scan data Reference Off On From the displayed list of stored scans a record is selected for use as a reference scan superimposed scan This can be highlighted or shown in the background in comparison with the actual measured scan Note This option is only available when there are stored scans with the same wavelength range and step Cursor Mode A To enter the wavelength spectrum and the scan interval Enables the user to switch the display back and forth between the scan data tables Select View wavelength absorbance and the graph of the curve Note Select View will be activated after the first reading To select Track or Peak Valley The selection for this menu item determines to which points on the graph the cursor moves Send Data To send Data to a printer computer or USB memory stick Type A Integral On Off The i
72. een with a fingernail fingertip pencil eraser or a stylus Do not press the screen with a sharp object such as the tip of a ball point pen Do not place anything on top of the screen to prevent damage or scratching on the screen e Press keys words or icons to select them e Use scroll bars to move up and down long lists very quickly Press and hold the scroll bar then move up or down to move through the list e Highlight an item from a list by pressing it once When the item has been successfully selected it will be displayed as reversed text light text on a dark background 5 1 2 Use of the alphanumeric keypad PS a gt won e sn vs ve m Cancel OK This display is used to enter letters numbers and symbols as needed when programming the instrument Unavailable options are disabled grayed out The icons on the right and left of the screen are described in Table 1 The central keypad changes to reflect the chosen entry mode Press a key repeatedly until the desired character appears on the Screen A space can be entered by using the underscore on the YZ key Note A USB keyboard with US keyboard layout or a USB Barcode handset scanner can be used for input see Section 9 on page 129 Table 1 Alphanumeric keypad Icon key Description Function ABC abc Alphabetic When entering alphabetic characters ex user entered units this key allows to toggle between
73. elength setup options Options Description More For further Options Store Off On With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings Wavelength To enter the measurement wavelength Use the alphanumeric keypad to enter the measurement 9 wavelength The entered wavelength must be in the range from 190 1100 nm This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed Timericon e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program Concentration Factor Multiplication factor for converting absorbance values into concentration values Concentration Resolution To select the position of the decimal point in the calculated concentration readings 87 Advanced Operations Single Wavelength V s Options Concentration Concentration Factor Off Resolution 0 01 Reading Mode Save as Single User Program Trans 25 SEP 2006 09 12 59 2 w Ret Instrument ero Options BUD Setup Table 12 Single wavelength setup options continued Options Description Single Reading Mode A reading is only displayed after a measurement has been carried o
74. ength Check Stray Light Absorbance Check Check ee el Verification 4 106 To obtain the software for the update from the Internet at www hach com 1 Goto http www hach com 2 Onthe DR 5000 product page click Lab System Software Software Update Downloads under the heading Downloads 3 Locate the appropriate download and follow the prompts for saving the file s to the USB memory stick 4 Onthe DR 5000 press Instrument Update in the System Checks menu 5 Connect the USB stick to the USB interface on the DR 5000 section 3 3 on page 14 Press OK The link is established automatically and the software is updated 6 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed For each optical check the measured results must be evaluated against user specific requirements The check options do not define tolerances 1 Press Optical Checks in the System Checks menu The Optical Checks menu contains programs for checking the wavelength accuracy stray light and photometric accuracy An optional test filter set Verification Kit Section 9 on page 129 containing six precision glass filters target values tolerances and instructions is available and is recommended as an aid for carrying out comprehensive in house instrument checks Advanced Operations 6 8 3 1 Wavelength check The Wavelength Check test is used
75. ent On the back side a fan is installed for cooling of electric components The ventilation system operates automatically Refer to section 7 2 1 on page 122 section 7 2 2 on page 123 and Figure 8 on page 124 for instructions on replacing the tungsten lamp and deuterium lamp UV 1 Switch the instrument off 2 Unplug the power cord DANGER The instrument must remain switched off until the change of lamp has been completed WARNING Burn Hazard Wait until the lamp cools down Contact with the hot lamp can cause burns 3 Use a screwdriver to remove the cover from the back of the instrument the screws may be slotted or cross headed 4 Place the cover and the attached fan carefully beside the instrument take special care with the fan cable 5 Push the spring Figure 8 on page 124 item 2 up and remove the tungsten lamp Figure 8 item 1 from the lamp compartment 6 Unplug plug contact Figure 8 item 7 from the tungsten lamp Important Note The lamp should only be held by the lamp socket Avoid touching the glass 7 Push plug contact firmly onto the new tungsten lamp 8 Insert the tungsten lamp into the lamp compartment Push the spring down 9 Check that the spring and the lamp socket are positioned correctly 10 Use a screwdriver to screw the back cover onto the instrument again 11 Plug in power supply again Maintenance 12 Switch the instrument on 13 Reset the Lamp History see sect
76. epends on the definition of the measurement Variables process and the formulas Input of the numerical values of the wavelengths factors constants etc Used to enter abbreviations and defined times for up to four timers Highlight the appropriate line and press Edit The timers are activated or deactivated with the control boxes on the left of the display In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered 6 1 2 1 Measurement process 72 The measurement process defines the handling and the measurements of the test e At which and how many wavelengths should measurements be completed How many absorbance measurements must be completed e When should the zero measurement and the sample measurement be completed e Are waiting times necessary between measurements Advanced Operations e Should individual program sequences be repeated The elements of a measuring sequence such as zero and sample measurements and the timer s reaction times waiting times etc are individually defined 6 1 2 2 Enter a new element of a measuring sequence Important Note Each component of the measurement process must be entered in the order in which it will be completed NY ONE EN Measurement Process 1 Highlight the Measurement Process line in the data overview and press Edit 2 Press Edit again
77. ert a brush or sharp object into Cell Compartment 1 to avoid damaging the mechanical components Drythe cleaned parts carefully with a soft cotton cloth e Take care not to scratch the display Do not touch the screen with ball pens pencils or similar pointed objects e Clean the display with a soft lint free and oil free cotton cloth Diluted window cleaner liquid can also be used CAUTION Potential Chemical Biological Exposure Hazards Use proper laboratory practices whenever there is a risk of chemical exposure 1 After performing a procedure clean glass cuvettes sample cells with cleaning agents 2 Afterwards rinse the cuvettes sample cells several times with tap water and then thoroughly with deionized water Important Note Glass cuvettes sample cells that have been used for organic solvents such as chloroform benzene toluene etc must be rinsed with acetone before being treated with cleaning agents In addition another rinse with acetone is necessary as a final treatment step before the cuvettes sample cells are dried 121 Maintenance 7 2 Lamp replacement 7 2 1 Tungsten lamp replacement 122 CAUTION The UV lamp generates UV light Do not look directly at an operating lamp without wearing UV protective eye glasses The Lamp compartment is on the left side behind the display and is provided with ventilation on the back side The tungsten and deuterium UV lamp are installed in the lamp compartm
78. factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off Note When a dilution is in effect the dilution icon will appear on the display Note If undiluted samples are used set the dilution factor off Chemical Form Some of the stored tests methods allow to select the chemical form and the measuring range Edit To modify an existing program Save as User Program To store the selected parameters as a User Program see section 6 1 on page 61 Recall Data Call up saved measurement data wavelength scans or time courses see section 5 3 on page 35 Instrument Setup Basic data of the instrument see section 5 2 on page 23 5 5 5 Sample blank 313 Chromium After blank value corr 28 APR 2005 11 52 56 0 120 gt Turbidity and color in the sample matrix can falsify the results of a photometric analysis The interference factors come from the sample or are created by reactions with the reagents The influence of turbidity and or color can be eliminated or reduced by taking a sample blank reading In the barcode mode a special cuvette vial TNT919 containing the sample blank is placed in cell compartment 1 after the sample reading has been taken and is automatically measured The sample reading is then corrected by adding
79. fe span For maximum life turn the lamp off only if it will remain off for at least 4 5 hours The lamp switches on automatically e ifalamp is needed for the selected program e if the instrument is operating inside the lamp s spectrum 1 Press Lamp Control in the Instrument Setup 2 Select On to switch on the VIS Lamp and or UV Lamp 3 Select Save in order to define a time interval for the burning time of the VIS lamp and or UV lamp 4 Press the field below Save to select the lamp burning time 2 Hours 4 Hours 8 Hours 12 Hours Cancel OK b br Wavelength A nm Standard Operations 5 Select the length of time the lamp will be switched on Note After this period of time the lamp automatically turns off after no measurement has been made outside the corresponding range UV VIS 6 Touch OK to confirm 7 Touch UV Switch key to select the wavelength value between 320 nm and 360 nm at which the instrument changes from the visible to UV source 8 Use the alphanumeric keypad to enter the maximum wavelength for UV operation 9 Touch OK to confirm and return to Lamp Control 10 Touch OK to confirm and return to Instrument Setup Note The selected program that requires a lamp has highest priority If the lamp in the Lamp Control was turned off the lamp will be turned on automatically if it is needed for the stored program 5 2 5 2 Effect caused by the lamp settings on the Measur
80. g OK again If measurements are to be completed at a number of wavelengths repeat the above two steps for each wavelength Note The entered measurement sequence is displayed key Elements of the measurement sequence that are to be repeated are placed in brackets The left bracket marks the start of the sequence that is to be repeated and the right bracket marks the end Note The key showing the right bracket remains inactive until a left bracket is entered 1 Press 2 Press the key that defines the sequence that is to be repeated Z or R Confirm with OK 3 Press New and then press Zeroing or Reading and use the alphanumeric keypad to enter the wavelength at which the measurement should be completed Press OK and confirm the input by pressing OK again 4 Press to end the sequence Note If an action such as a zero measurement recurs at different stages of a measurement sequence the series of actions is numbered sequentially e g Z1 Z2 etc User oct Measurement Process Advanced Operations Delete an element of a measuring sequence Select the appropriate line and press Delete The element is deleted Insert an element of a measuring sequence Select the line in the measuring sequence where the insertion is be made and press New A new element can be entered at the selected position When the input is complete press OK in the Measurement Process displ
81. ge nl HACH LANGE S L U Edif Arteaga Centrum C Larrauri 1C 2 Pl E 48160 Derio Vizcaya Tel 34 94 657 33 88 Fax 34 94 657 33 97 info hach lange es www hach lange es HACH LANGE S R O Roln cka 21 SK 831 07 Bratislava Vajnory Tel 421 0 2 4820 9091 Fax 421 0 2 4820 9093 info hach lange sk www hach lange sk HACH LANGE SU ANAL Z S STEMLER LTD T Hilal Mah 75 Sokak Arman Plaza No 9 A TR 06550 ankaya ANKARA Tel 90 0 312 440 98 98 Fax 90 0 312 442 11 01 bilgi hach lange com tr www hach lange com tr 131 Contact Information 132 Section 11 Limited Warranty Hach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual In the event that a defect is discovered during the warranty period Hach Company agrees that at its option it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges Any product repaired or replaced under this warranty will be warranted only for the remainder of the original product warranty period This warranty does not apply to consumable products such as chemical reagents or consumable components of a product such as but not limited to lamps and tubing Contact Hach Company or your distributor to initiate warranty support Products may not b
82. h configuration The reading currently shown in the display is then stored Note When the instrument s memory data log is full the oldest data are automatically deleted allowing the new data to be stored 1 Press Recall Data in the Main Menu 2 Press Data Log A listing of the stored data is displayed 3 PressFilter On Off 35 Standard Operations j i e 19 52 Filter Settings Filter C off SE Sample ID Operator ID ww 05 lt All gt y i Start Date Parameter um lt All gt lt All gt Data Log 4 15 SEP 06 09 15 04 WW 05 02 a 3 93 mg l PO4g7 P 15 SEP 06 09 15 40 WW 05 03 3 95 mg l PO47 P 15 SEP 06 10 03 24 WWw 05 04 dde mg L AP 15 SEP 06 10 08 00 ww 0O5 05 0 000 mg L AI Filter View a On Details Options 5 3 1 3 Send data from the data log Reca Matan Mate to 4 Send Data g 4 Single point C Filtered data 4 C All data 52 TEUER 36 4 The function Filter Settings is used to search for specific items 5 Highlight On to turn on the filters to select data by Sample ID e Operator ID e Start Date e Parameter or any combination of the four 6 Press OK to confirm the selection The chosen items are listed 7 Press View Details to get more information Data is sent from the data log as CSV Comma Separated Value files through a USB memory stick to a file named DATALOG The file can then be processed using a s
83. he entered data The data can be stored under any data point Measurement sequence Formula Timer etc 2 Press the PC amp Printer icon to send the program data to a printer or to a USB memory stick connect the USB memory stick to the USB interface first Note The test data will be formatted in the preinstalled PrgData folder as a csv and as a Ist file 3 To transfer the program data from one instrument to another create a new folder on the USB memory stick named dbhl and copy the Ist files into this folder see section 6 8 2 on page 106 6 1 3 Select a user program User Programs 950 User Program Program Options Advanced Operations Press User Programs in the Main Menu to view an alphabetical list of user programs with program numbers The User Programs list will appear Note Use the scroll bar to scroll through the list quickly Note Highlight the selection by pressing it or press Select by Number to search for the program by number 2 Press Start to run the program 6 1 4 Add edit and delete user programs from the favorites list User Programs 950 User Program Program Options Add to tem Favorites Edit Delete HUM The most frequently used tests methods in the User Program menu can also be added to the list of favorites to simplify their selection Press User Programs in the Main Menu The User Programs list will appear Highlight the selection
84. he evaluation form reverts to the standard setting An alternative way of changing the standard setting 1 1 4 Press Options More Chemical Form Select the Chemical Form Note The stoichiometric conversion of the measurement result is carried out automatically Note The selected Chemical Form will appear on the display Test results will be calculated and displayed in this chemical form Insert the sample cuvette or blank depending on the working procedure into the cell compartment In the result display press Options More Chemical Form A list of available evaluation forms appears Select the new default setting Press Save as Default The current result and all further measurements will be displayed in the new chemical form 47 Standard Operations 5 4 8 Run a reagent blank Reagent Blank Adjust to 0 000 mg L AI Cancel OK en 10 Aluminum Alumin VIS 522 nm 0 100 7 a Options 48 Some of the stored tests methods include the Reagent Blank function This enables the reagent blank value to be measured and then taken into account in calculating the measurement result Measurement analysis of a reagent blank 1 Prepare the test method in accordance with the working procedure Instead of a sample deionized water is used to determine the reagent blank value 2 Select the test If required by the working procedure insert the cell with deionized wate
85. he remote function is only for monitoring the data transfer renes een 5 2 7 Password The Password menu contains a variety of security settings to control access to various functions For example prevent unauthorized changes to stored programs or instrument configurations Password 1 Press Password in the Instrument Setup menu Security off C On Set Password Security List 2 In order to highlight the Security List assign a password Press Set Password 3 Use the alphanumeric keypad to enter a new Password up to 10 characters each and press OK to confirm The access to the Security List is activated 32 Instr na ot O ot ee Password Security off C On Set Password Security List Inst mast Lae Security List New Program v Edit Program L PECE DETA Doma Cancel OK Standard Operations 4 Press Security List to lock various functions for unauthorized users 5 Highlight the desired functions to control 6 Confirm the Security List with OK to return to the Password menu 7 Press On to highlight the new settings of the Security List 8 Enter the new Password again to confirm 9 Press OK to return to Instrument Setup Note The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting 1 Press Password in the Instrument Setup 2 Use the alphanumeric keypad to enter the for
86. ic settings can be entered Operator ID Sample ID Date amp Time Display amp Sound Lamp Control PC amp Printer Password and Module Options 22 5 2 Instrument Setup mode Instrument Setup Operator ID Sample ID lt Off gt Om Date amp Lor Display amp Time i Sound ee Lamp PCR Control Printer S Carousel a Password Options 5 2 1 Operator ID Instr nanena CO mde ons Operator ID Standard Operations 1 Select Instrument Setup in the Main Menu A selection of functions appears in order to configure the functions of the instrument This display appears when the multi cell holder is installed or the 13 mm round cuvette vial compartment is used If the imstrument recognizes a Sipper module or a Peltier module the Carousel Options key is replaced by Sipper Options or Peltier Options Optional modules for the DR5000 e Carousel Holder Sample Changer e Pour Thru Cell module e Sipper module e Peltier module Temperature control module Refer to the corresponding manuals instruction sheets for more information Use this option to enter up to 30 sets of operator initials up to 5 characters each into the instrument This feature helps record which operator measured each sample 1 Press Operator ID in the Instrument Setup 2 Press New to enter a new Operator ID 3 Use the alphanumeric keypad to enter a new Operator ID 4 Press OK to confirm 5 The
87. ing Mode Single Wavelength UV 320 nm Lamp Warmup 10 JAM 2008 09 08 41 A Exit Cancel Options UV Lamp switches on manually The icon UV Lamp appears in the display and flashes The instrument will display Lamp Warmup This takes about 3 minutes 27 Standard Operations Single Wavelength VO 320 nm 0 000 s 10 JAM 2008 09 10 07 Exit Zero Options 28 When the lamp is warmed up and ready the UV icon stops flashing Note If both lamps are On the icon UV VIS is displayed in the selected Measurement Mode Note Alternatively you can touch the UV or VIS Lamp icon on the measurement screen to change the actual setting Standard Operations 5 2 6 PC and printer The instrument is provided with 3 USB interfaces which are located on the back of the instrument see Figure 1 on page 14 These interfaces can be used for exporting data and graphics to a printer updating data and for data communication to a personal computer These interfaces can be used for the connection of a USB stick an external USB keyboard or a USB Barcode handset scanner Note A USB hub may be used to connect several accessories at a time A USB memory stick is used to upgrade data and software see section 6 8 2 on page 106 Important Note A screened USB cable must not be longer than 3m Table 3 USB connector USB Interfaces Description This USB interface is only intended to connect the instrument
88. ion 6 8 5 on page 115 7 2 2 Deuterium lamp UV replacement 1 Switch the instrument off 2 Unplug the power cord DANGER The instrument must remain switched off until the change of lamp has been completed WARNING Burn Hazard Wait until the lamp cools down Contact with the hot lamp can cause burns 3 Use a screwdriver to remove the cover from the back of the instrument the screws may be slotted or cross headed Place the cover and the attached fan carefully beside the instrument take special care with the fan cable Unplug the deuterium lamp Figure 8 on page 124 item 4 from the socket by pushing down on the safety contact Use a screwdriver to unscrew the two fastening screws Figure 8 item 3 the screws may be slotted or cross headed out of the socket Holding the lamp socket lift the deuterium lamp up out of the lamp compartment remove the complete unit including the cable Carefully insert the new deuterium lamp into the lamp compartment Important Note Do not touch the glass envelope on the new lamp If it is touched clean with alcohol 9 10 11 12 13 14 Screw in both fastening screws again until they are finger tight Insert the deuterium lamp in the socket so that the safety contact clicks into place Use a screwdriver to screw the back cover onto the instrument again Plug in power supply again Switch the instrument on Reset the Lamp History see section 6
89. is own requirements Single Wavelength Single wavelength measurements are Absorbance measurements The light absorbed by the sample is measured in absorbance units Transmittance measurements 95 The percentage of the light that passes through the sample and reaches the detector is measured Concentration measurements A concentration factor can be entered to enable the measured absorbance values to be converted into concentration values Multi Wavelength In the multi wavelength mode absorbance Abs or percentage transmittance 96 T is measured at up to four wavelengths and absorbance differences and absorbance relationships are calculated Simple conversions into concentrations can also be carried out Wavelength Scan A wavelength scan shows how the light from a sample is absorbed over a defined wavelength spectrum This function can be used to determine the wavelength at which the maximum absorbance value can be measured The absorbance behavior is displayed graphically during the scan Time Course The time scan records the absorbance or 96 transmittance at a wavelength over a defined time System Checks Recall Data The system checks menu offers a number of options including optical checks output checks lamp history instrument update service time and instrument backup Stored data can be recalled filtered sent and deleted Instrument Setup In this mode user specific or method specif
90. le reading sssee 89 6 4 3 Take single wavelength measurements continuous readings ooocccccnnocccccnnonocccccnnnonnncncnnanancncnn nan nncncnnnn 89 6 5 Multi Wavelength mode measurements at more than one wavelength sse 90 6 5 1 Set the reading mode at different wavelengths ssssessssseeeemeee eene 90 6 5 2 Complete a measurement in the multi wavelength mode sss 93 6 6 Wavelength Scan mode recording of absorbance and transmittance spectrums ssssssessss 94 6 61 Setup the wavelength Scan sa e e IR di 94 6 6 2 Wavelength scan reading iiie e dali 97 6 6 2 1 Navigation of the wavelength scan graph or a wavelength scan analysis sssss 98 6 6 3 Work with reference scans ccccceeceeceneceeeeeeceneeeeeeccaneeesseedaneendauacdaceeeeeceaeeeededaneeeeeeeedaeenseeeaneceedeedaneneeees 99 6 7 Time course of absorbance transmittance ssssssssssssseneeneneeeenen nennen eren nennen nnne enn 101 6 7 1 Time course setup parameters ssssssssssssssseseeeeeneeenmer e enne RR RRA nennen nennen en nennen nennen 101 6 7 2 Time course scan reading ocoioinnninnnninnoninici sitientes eiit bns niit hehe dites A A nns innen 102 6 73 Analysisof tme couse data iri mt ee ed ne tede o RARE ERE 103 6 7 3 1 Navigation of a time scan or a time scan analysis ssssssssee eee
91. mal places from the displayed list and press Next 63 Advanced Operations ae LU ES no aR el i Units K1A1 KzAz z Aa Az I 600 700 i Ka Kz 1 0000 1 0000 64 Chemical form Enter the chemical formula used in the display to represent the analysis parameter Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings If the Multi Wavelength mode is selected the following parameters can be defined Units Select the required unit from the list and press Next Note Units of measure not included in this list can be added in the edit program under Program Options Edit Select Units Edit and then New Absorbance formula multi wavelength program type The Absorbance Formula menu is used to define the wavelengths and the coefficients used in the formula The absorbance formula defines the calculation for the multi wavelength measurement Press the appropriate key to edit the input Press the Formula key In the displayed list select the formula for the program and press OK to confirm K1A1 KzMz KiAat KzAz KzAz KaAat KzAz KsAzt Kaie KiAa KzAz K141 K242 KzA3 K1A1 K242 K252 KaM4 Cancel OK H KaAat KzAzt KzAzt Kata da Az ha ha 600 700 600 500 al Ka Ke Ka Ka 1 0000 1 0000 1 0000 1 0000 Cancel OK User Pia ree nen n a Concentration Resolution 0 01 C
92. ment of a measuring sequence ssssssee emen 73 6 1 2 3 Enter the calibration formula evaluation formula se mem 75 6 1 2 4 Enter variables 2 nice tide ascendet e eset a eaa da 78 6 1 2 5 Save a free programming user program sssssssssssseeeene nennen enne 78 6 1 3 Selecta user program ee crederet dae d da E e ace dett ended Pee La Air Aen 79 6 1 4 Add edit and delete user programs from the favorites list sssssssssssseeeeneee 79 6 1 4 1 Add to Favorites 2 adie eed eei diee eel p rene tn le AA 80 OA ZE ta eene A ie dade es eade te d esa RE rita ER sea ERE eee 80 0 1 4 9 Delete ene A ROI aate REM 80 6 2 Favorite Programs i ooi tn n Het eire e Mt ERR tele e OD ee epe ens 81 6 2 1 Recall a favorite program Ite Ue eU LO HERR DER RR eR Rant CREE SELLER Rae FRE Led atlas 81 6 2 2 Delete a favorite program 2 eite he b t linealidad irrita 81 6 3 Standard Addition monitoring checking results ooonnoncccnnnnnonicicnnnaccncnccnnnancnn conan nn nnrcnnnn nn narran rr nennen nnns 82 6 3 1 Complete a standard addition sse merenti nnne nnne nens 83 Table of Contents 6 4 Single Wavelength absorbance concentration and transmittance measurements sssuussss 87 6 4 1 Set up single wavelength mode sse nemen nennen nennen eren nennen nennen enn 87 6 4 2 Take single wavelength measurements sing
93. ment users must now return old or end of life equipment to the Producer for disposal at no charge to the user Note For return for recycling please contact the equipment producer or supplier for instructions on how to return end of life equipment producer supplied electrical accessories and all auxiliary items for proper disposal nt pb This symbol indicates that the instrument contains a Class 1 LASER device LASER CLASS 1 Data 0 3 mW X 650 nm General Information 2 1 3 Class 1 LASER A Class 1 LASER is installed in this instrument Class 1 LASERS are products where the radiant power of the LASER beam accessible the accessible emission is always below the Maximum Permissible Exposure value Therefore for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur Exposure to the beam of a Class 1 LASER will not result in eye injury Class 1 LASERS may therefore be considered safe However Class 1 LASER products may contain LASER systems of a higher Class but there are adequate engineering control measures to ensure that access to the beam is not reasonably likely Examples of such products include LASER printers and compact disc players CDRH assession number 0510555 02 Data 0 3 mW wavelength 650 nm 2 1 4 Chemical and Biological Safety 2 1 5 Source Lamp Safety 10 DANGER Potential Chemical Biological Exposure Hazards Handling chemical samples standards
94. mer Password and press OK to confirm 33 Standard Operations Inst ot Lots Security C Off On Set Password Security List Password ox 34 3 Press Off to deactivate the settings of the Security List 4 Press OK to return to Instrument Setup Note Use this function to delete the former Password or to enter a new one Standard Operations 5 3 Store recall send and delete data 5 3 1 The Data Log 5 3 1 1 Auto manual data storage 10 Aluminum Alumin 15 SEP 2006 14 49 13 Read 15 SEP 06 14 51 25 0 000 mg L Al 15 SEP 06 14 51 56 0 114 mg L AI 18 SEP 06 09 16 39 0 110 mg L al 18 SEP 06 10 59 08 0 118 mg L AI 18 SEP 06 11 01 14 0 000 mg L AI Main Filter View Menu Off Details Options The Data Log will store up to 1000 readings taken in the modes Stored Programs User Programs Favorite Programs Single Wavelength and Multi Wavelength A complete record of the analysis is stored including the Date Time Results Sample ID and Operator ID The data storage parameter indicates whether data are to be stored automatically or manually in which case the user has to decide which data to store 1 Press Store On Off in the Options menu e With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored However this setting can be changed to Store On in the result display throug
95. mi iwi 122 7 2 1 Tungsten lamp replacement 0 00 2 ccceeeeeeeceeeeecae cee ee eee ee cette ee caaaaaecaeeeeeeeeeeeeeeeedseccaeaaesaeeeeeeeeeeesetsenenaeees 122 7 2 2 Deuterium lamp UV replacement sssssssssssseseeeeenen enne enne nn nnn rnnt nere nnne nnns 123 TI FUSE replacemaent 2 2 eet bd Ded de tt pedet retenu red de da cd 124 7 4 Filter pad maintenarnce xerit deter cte daa goa Ha E e t ta REOR LR es he lent ote det ets 125 7 4 1 Filter pad replacement A a du et e E ERR ge oed agian ta 125 Section 8 Troubleshooting rro leo ie abeo lle tenui tala ces EL EARE e AC aoc KE Mte EROR 127 Section 9 Replacement Parts n o ote p E HL e tae Me A M E d qu a EP eaae eiaa 129 Section 10 Contact Information sssssssssssssseee eem ee eee rra 131 Section 11 Limited Warranty onore n a 133 jp 135 Table of Contents Section 1 Specifications Specifications are subject to change without notice Performance specifications Operating mode Transmittance Absorbance and Concentration Source lamp Gas filled Tungsten visible and Deuterium UV Wavelength range 190 1100 nm Wavelength accuracy 1 nm in Wavelength Range 200 900 nm Wavelength reproducibility lt 0 1 nm Wavelength resolution 0 1 nm Wavelength calibration Automatic Wavelength selection Automatic
96. n 0 000 Abs 6 8 3 5 Drift check The Drift Check test is used to test the stability of the instrument Note The Drift Check runs 1 hour wavelength A nm 1 Press Drift Check in the Optical Checks menu 2 Press A to input the wavelength 3 Input the wavelength and press OK to confirm 9 tic Abanke E Drift Check 4 Press Start H Wavelength 546 nm 112 Advanced Operations 8 tic Abanke p Drift Check P wavelength 546 nm 0 0002 Abs 59 56 Cancel 8 tic Abank p Drift Check B Wavelength 546 nm m Total Drift 0 0010 FED EMT Initial Reading 0 0000 Abs a Final Reading 0 0005 Abs 0 15 min 0 0005 UP Era Last Abs 0 0001 Abs 6 8 3 6 Verification kit Optic Abank Verification Kit E Nominal Verification Values enn 5 The Drift Check runs 1 hour This test takes a reading every minute for one hour Every 15 minutes linear regression is used to calculate the slope rate of change for the previous 15 minute interval 6 The last reading and slope for each 15 minute interval are displayed At the end of the hour the overall values are calculated and displayed The test proceeds to completion 7 Press Cancel to return to Optical Checks The Verification Kit LZV 537 see Section 9 on page 129 is designed for periodic monitoring of scattered light photometric accuracy and the wavele
97. n and the time interval between the collection of data Time amp Interval points A To input the wavelength setting View Table Graph To display readings in absorbance transmittance or concentration This can be changed after sample data are collected Scale In the automatic scaling mode the y axis is automatically adjusted so that the total scan is displayed Scale amp Units The manual scaling mode allows sections of the scan to be displayed Units Choice of absorbance or transmittance Send Data To send Data to a printer computer or USB memory stick Type A Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 35 Instrument Setup Basic data of the instrument see section 5 2 on page 23 101 Advanced Operations ute AN Time Interval 4 n nm Total Time som Reading Interval mu eue Optic E Units Abs Scale amp Units Scale Auto C Manual 0 300 Abs w 3 500 Abs d Cancel OK en 6 7 2 Time course scan reading Time Course 400 nm 0 25 SEP 2006 10 18 51 zero Options 102 Time amp interval 1 Press Time amp Interval in the Options menu 2 Input the total time and the reading time and press OK to confirm Note In total 500 measuring steps are possible To select a total time and a time interval that would cause this number of measurements to be exceeded the time interval is
98. n of Chemical Form 1 and press OK to confirm 4 Press Store to save the program data Press Cancel to return to the Main Menu 71 Advanced Operations 6 1 2 Free programming program type User Program 951 Name FP Cancel Free Programming is an advanced option for entering original user developed methods When the Free Programming option is selected an overview of the specifications of the programmed test is displayed Each input option can be modified to develop the user method Refer to Table 10 for more information To modify an input option select the appropriate line and press Edit Important Note Perform steps 1 6 of Programming a New User Method section 6 1 1 on page 61 before proceeding with Free Programming Highlight the line containing the program point that is to be edited or defined and press Edit Table 10 Definitions of the program points Program point Name Description Name of the analysis parameter Version An abbreviation or version number assigned by the user is entered here Measurement Process Exact definition of the test the number of wavelengths at which measurements are made the number of absorbance measurements needed the keys to be used any waiting periods between measurements etc Timer 1 Timer 2 Timer 3 Timer 4 Formula Definition of the formulas with which the test result is calculated The number of variables shown in the display d
99. nce on the measurement program 7 8 When the data have all been entered and the measurements have all been completed press Graph The linear curve corresponds to the standard setting Press Next Curve to display the polynomial 2nd order curve Press Next Curve again to display the polynomial 3rd order curve nen Calibration C Enter Values C Read Standards Enter Formula User Munmun nen Enter Formula Advanced Operations 9 Press Force 0 to change the setting from Off to On The curve then passes through the origin of the coordinate system Note This may have an adverse effect on the correlation coefficient r 10 Press Table to display the table again 11 When the table has been completed and the curve type has been chosen press Done when the graph is displayed or Exit when the table is displayed Go to section 6 1 1 4 on page 70 Calibration by entering the formula 1 Press Enter Formula and press Next 2 Press the formula key A list of available formulas linear and 2nd and 3rd order polynomial is displayed Up to 4 coefficients can be entered depending on the selected formula Press the required formula 3 Depending on the selected formula the required coefficients a b c are displayed Press the coefficient keys and enter the corresponding values via the alphanumeric keypad After each entry press OK to confirm Note The coefficients can have 5 digits
100. ncentration shown above the curve is the estimated concentration of the sample without the added standard Note In the curve menu the name on the Curve key switches to Table Press Table to display all the data in the table again Press Ideal line to display the relationship between the added standard solutions and the ideal line detection rate 100 Advanced Operations 6 4 Single Wavelength absorbance concentration and transmittance measurements The Single Wavelength mode can be used in three ways For sample measurements at a single wavelength the instrument can be programmed to measure the absorbance transmittance or concentration of the analyte Absorbance measures the amount of light absorbed by the sample in units of absorbance transmittance measures the percent of the original light that passes through the sample and reaches the detector Turn the concentration factor on to select a specific multiplier for converting absorbance readings to concentration In a graph of concentration versus the absorbance the concentration factor is the slope of the line 6 4 1 Set up single wavelength mode Press Single Wavelength in the Main Menu Press Options for Parameter Setup Single Wavelength VIS XX Options 25 SEP 2006 Concentration Concentration Factor Off Resolution 0 01 Reading Mode Save as Single User Program Trans 09 12 59 2 w Ret Instrument ero Options eturn Setup Table 12 Single wav
101. nd the UV Lamp will be reset 4 Press OK to return to System Checks 115 Advanced Operations 6 8 6 Factory service aES SSe Service Code voe Ed ES 6 8 7 Service time Last Service 26 JUN 2006 Next Service 26 JUN 2007 12 Month Cancel OK Systr Abanke Next Service E C 3 Month C 6 Month 12 Month de Cancel OK 116 The Factory Service menu is password protected This menu is not intended for customer use In order to ensure a regular inspection an automatic memory reference for the service times can be entered After switching the instrument on this memory reference will be activated and indicated at the appropriate time 1 Press Service Time in the System Checks menu 2 Select On and then Last Service to enter the date of the last inspection 3 Press OK to confirm 4 Select Next Service to determine a specific period of time up to the next inspection 5 Press OK to confirm Advanced Operations If the next service is due the message Next service is due is Sarv ci Lus displayed after switching on the instrument 6 Press OK to return to the Main Menu Next Service Contact the manufacturer or distributor to arrange an appointment is due for the next service 6 8 8 Instrument Backup Before the next service date the Instrument Backup menu offers the possibility to store all programs me
102. ngth accuracy of the spectrophotometers When results exceed allowable tolerances given in the quality control certificate contact the manufacturer 1 Press Verification Kit in the Optical Checks menu 2 Press Nominal Values 113 Advanced Operations Optic Abanke Set Number Valid until KV450 3 NG9 1 NG5 2 NG11 2 Ho BG20 2 Cancel Edit OK Nominal Yalues 0004 19 OCT 2007 gt 2 8 Abs 1 493 Abs 0 591 Abs 0 302 Abs 360 9 nm 807 0 nm ee m a Verification Kit a Nominal Verification Values Cancel Optic Abanke Verification Please remove cuvette and touch Start N Stray Light Check cancel m 114 Press Edit An automatic menu guidance queries values filters wavelength nominal values and tolerances given in the quality control certificate to the following specifications e Stray Light e Photometrical accuracy e Wavelength accuracy Press OK when all values are entered and the overview is displayed Press Verification Insert the Multi Cell Holder in the cell compartment in such a way that the cell opening for 10 mm square cuvettes cells is at the front Secure the holder with two locking screws 7 Remove any cuvettes cells from the cell compartment close the cell compartment and press Start Optic Abanke Verification P Stray Light Check Please insert filter K 450 3 and touch Next ict ChaaL DEM
103. nt Make sure that the protection provided by this equipment is not impaired do not use or install this equipment in any manner other than that specified in this manual 2 1 1 Use of hazard information DANGER Indicates a potentially or imminently hazardous situation which if not avoided will result in death or serious injury WARNING Indicates a potentially or imminently hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury Important Note Indicates a situation which if not avoided may cause damage to the instrument Information that requires special emphasis Note Information that supplements points in the main text 2 1 2 Precautionary labels Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger or caution statement in the manual This symbol if noted on the instrument references the instruction manual for operation and or safety information This symbol if noted on the instrument indicates a hot surface Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after 12 August of 2005 In conformity with European local and national regulations EU Directive 2002 96 EC European electrical equip
104. ntegral gives the area and the derivative of the integral gives the original function 94 Advanced Operations Wavelength Scan MEROS Options Cursor Mode cH Send 3 500 Track Data Abs cas Scale amp Units Reference off A 0 300 400 nm 25 SEP 2006 11 10 07 Main v Instrument Menu Zero Return Setup Table 14 Wavelength scan setup options continued Option Description Scale In the automatic scaling mode the y axis is automatically adjusted so that the total scan is displayed Scale dns The manual scaling mode allows sections of the scan to be displayed Units Choice of absorbance or transmittance Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 35 Instrument Setup Basic data of the instrument see section 5 2 on page 23 Wavyr onan ute AN ean nm Wavelength Range Setting wavelength 2 E 600 1 Press the A key in the Options menu to select the wavelength 7 range and the wavelength step Step 2 Press the upper left key to open the numeric keypad and select o ud the minimum wavelength Press OK to confirm C 2nm C 5nm 3 Press the upper right key to open the numeric keypad and i Pascal OK h select the maximum wavelength Press OK to confirm ons Note Do not select the same wavelength for minimum and maximum 4 Highlight the required wavelength step Note Scan
105. ntration factor allows selection of a specific multiplier for converting absorbance readings to concentration In a graph of concentration versus the absorbance the concentration factor is the slope of the line Concentration is calculated using a single factor for each wavelength which is input by the user 6 5 1 Setthe reading mode at different wavelengths Multi Wavelength 25 SEP 2006 Press Multi Wavelength in the Main Menu Press Options for Parameter Setup Options Concentration Concentration Factor Off Resolution 0 01 Absorbance Save as Formula User Program Trans 11 03 12 Instrument Read eac Setup Zero Table 13 Multi wavelength setup options Options More Description For further Options Store Off On With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings Wavelength Timer icon Concentration Factor To enter the measurement wavelength Use the alphanumeric keypad to enter the measurement wavelength The entered wavelength must be in the range from 190 1100 nm This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the time
106. ntration of a sample that has been diluted by a known ratio For example dilution factor can bring the analyzed concentration within the test range Press Options More Dilution Factor The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off Press OK to confirm 45 Standard Operations ES ute A 22 nm Store Data Store present reading again Cancel OK E 10 Aluminum Alumin VIS 522 nm Coit n i Nd 15 SEP 2006 14 50 01 e a Zero Options 5 4 6 Runa standard adjust Standard Adjust Current Reading 0 110 Adjust to 0 127 mg L AI H Cancel Adjust 46 3 Press Return to return to the result display 4 Confirm Store present reading again to save the current measured value with the dilution factor Note When a dilution is in effect the dilution icon will appear on the display Note If using undiluted samples set the dilution factor off again The Standard Adjust function allows the calibration curve for a stored program to be adjusted based on analysis of a known standard solution The Accuracy Check section of written procedures often suggests a standard solution concentration for this purpose Read a standard before setting Standard Adjust to On 1
107. o connect several accessories at a time Note USB cables must not be longer than 3 meters 10 feet These USB interfaces enable data and graphics to be output to a Printer and a PC and upgrade of instrument software see section 6 8 2 on page 106 Figure 1 Interfaces 1 USB type A 4 Fuse 2 USBtype B 5 Plugin power supply 3 On Off switch 14 3 4 Installation Cell compartment Multi Cell Holder 100 mm Cell Adapter optional Figure 2 Cell compartment 1 Cell compartment 2 Cell compartment for 13 mm or 16 mm round cuvettes vials only The DR 5000 Spectrophotometer comes equipped with a Multi Cell Holder Figure 3 on page 16 which is the Standard Holder provided with each instrument The Multi Cell Holder can accommodate the following cell types 10mm square cells e 20 50 mm rectangular cells e 1 round cells e 1 square cells Only one cell type can be used for a measurement On the top and bottom of the Multi Cell Holder are a variety of openings to accommodate different types of cells Beside each opening is printed the type of cell for which it is intended Optionally there is a 100 mm adapter available 15 Installation Figure 3 Multi Cell Holder Top and Bottom 1 10 mm square cell 4 1 round cell 2 20 mm rectangular cell 5 1 square cell 3 50 mm rectangular cell 3 4 1 100 mm adapter optional Figure 4 100 mm Cell
108. ogram Options menu 61 Advanced Operations Program Number 950 999 Name nen Program Name Program Number Specific test number with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu 2 Use the alphanumeric keypad to enter a program number between 950 and 999 The lowest available number appears automatically Press OK Note If the program number is already assigned to another user program a message appears asking whether the existing program should be replaced Press OK to overwrite the existing program Program Name user Program 4 Use the alphanumeric keypad to enter a program name The e praet Abc DEF GHI CE name can be a maximum of 28 characters long Cali EN lol por 5 Press Back to go back to the previous program point or press el vo Next to continue with the input of the program data j 123 STU vwx YZ_ Cancel Back Next i Program Type Program Type Erie eosin wench ab it 6 Select the required option Table 8 and press Next C Multi Wavelength 7 If the Single Wavelength section 6 1 1 1 on page 63 or Multi C Free Programming Wavelength section 6 1 1 2 on page 64 is selected define the unit wavelength absorbance formula wavelength Ax concentration factor Kx concentration resolution
109. or subtracting the blank value The final result is displayed with the message After blank value corr a Options Some barcode tests do not require a sample blank value to be determined as turbidity and color are dealt with during the test 53 Standard Operations procedure Other barcode tests use the reagent test vials to determine the sample blank Please refer to the Hach procedure for method specific instructions 5 5 6 Update edit barcode tests 5 5 6 1 Manual update of a barcode test Main Barcode controlnumber Please update program data Using the data provided in the barcode the instrument automatically sets the measurement wavelength and factors If a discrepancy is detected between the barcode data and the stored data or a new test is identified the instrument requests an update Data types are described in Table 6 Program point Table 6 Program update options Description Name Name of the analysis parameter Version An abbreviation assigned by the user or the version number etc is entered here Measurement process Exact definition of the test the number of wavelengths at which measurements are carried out the number of absorbance measurements needed the keys to be used any waiting periods between measurements etc Formula Variables Definition of the evaluation formulas with which the test result is calculated The number of variables shown in
110. preadsheet program The file name will be formatted as DLYear_Month_Day_Hour_Minute_Second CSV To send data to a Printer see section 5 2 6 2 on page 31 1 Plug in the USB device Figure 1 on page 14 2 Press Recall Data from the Main Menu Press Options and then the PC amp Printer icon 3 Select the data to send to the memory stick and press OK Note The number in parenthesis is the total number of data sets assigned to this selection To send measurement data to a PC The optional HACH Data Trans software must be installed on the PC see section 5 2 6 3 on page 31 Standard Operations 5 3 1 4 Delete stored data from the data log Delete Data Single point C Filtered data 4 C all data 52 mag 4 1 Press Recall Data in the Main Menu Press Data Log gt Options gt Delete Highlight Single Point or Filtered data or All data and press OK to confirm Note The number in parentheses is the total number of data sets assigned to this selection 37 Standard Operations 5 3 2 Store recall send and delete data from wavelength scan and time course The instrument can store 20 Wavelength Scans and 20 Time Course Data sets The data can be stored manually at the user s discretion after viewing the data 5 3 2 1 Data storage from wavelength scan or time course Wavelength Scan 1 Press the Store icon in the Options menu after a reading is taken A 0 200 400 Integral 279 00
111. press Next Enter Values C Read Standards C Enter Formula 66 Advanced Operations Calibration Values 2 To enter the standard concentrations and corresponding absorbance values in the displayed table press the symbol Use the alphanumeric keypad to enter the values Press OK and enter the corresponding absorbance value Press OK The entered data are displayed in the table Repeat the sequence for each data point to enter it 3 To change a value in the table highlight the appropriate line press the unit key e g mg L or Abs and enter the changed value via the alphanumeric keypad SS 4 When the data have all been entered press Graph Note The correlation coefficient r2 is shown on the left below the axes 4 00 m g L Curve Fit r 21 0000 Calibration Values 5 The linear equation corresponds to the standard setting Press C a b4 cA Next Curve to display the polynomial 2nd order curve Press Next Curve again to display the polynomial 3rd order curve 6 Press Force 0 to change the setting from Off to On The curve now passes through the origin of the coordinate system Note This may have an adverse effect on the correlation coefficient 2 4 00 mg L Curve Fit r 1 0000 7 Press Table to display the table again 8 When the table has been completed and the curve type has been chosen press Done when the graph is displayed or Exit when the table is displ
112. r 1 F2 Factor 2 A1 Wavelength 1 U1 Conversion Factor 1 for the first chemical form U2 Conversion Factor 2 for additional chemical forms etc Timer 1 Timer 2 Timer 3 and Timer 4 The timers are activated or deactivated with the control boxes on the left of the display In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered 5 5 6 2 Update an existing barcode test Standard Operations Note Only in very few cases does the revision of a test require all test specifications to be updated Program 341 Name Nitrite Cancel Options 1 2 Option 1 Manual update of test data The instrument needs to be at the Main Menu Insert the blank or sample cuvette vial depending on the working procedure in cell compartment 1 and close the cell compartment After Barcode controlnumber a data overview is displayed including the specifications of the test that is to be revised Note The working procedures of the corresponding test contains the new data Wavelength Factors Measurement Ranges Conversion Factor for chemical Form etc Highlight the corresponding line and press Edit When finished programming press Store and then Cancel to return to the User Programs menu Press Start to complete the test with the new data Option 2 Manual check revision of test data If an incorrect ent
113. r has no influence on the measurement program Multiplication factor for converting absorbance values into concentration values Concentration Resolution To select the position of the decimal point in the calculated concentration readings Absorbance Formula Calculation basis for evaluating samples Save as User Program To store the selected parameters as a User Program see section 6 1 on page 61 90 Advanced Operations Multi Wavelength Concentration Concentration Factor Off Resolution 0 01 Absorbance Save as Formula User Program haoo Asoo 25 SEP 2006 11 03 12 e Instrument Options Return Setup Table 13 Multi wavelength setup options continued Options Description Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 35 Instrument Setup Basic data of the instrument see section 5 2 on page 23 Optic Absorbance Formula Absorbance formula n KAEA 1 Press Absorbance Formula R E 2 The formula selected in the top key determines the number of 400 IE 500 l wavelength and coefficent keys that will appear below To 1 m change the absorbance formula press the top key select a 1 Bae ies 4 RN formula from the displayed list and press OK When a new formula is selected the number of variables below changes to l GanEsl match The following formulas are avail
114. r into the cell compartment Press Zero 3 Insert the prepared sample cell into the cell compartment Press Read The result is displayed 4 Press Options gt More gt Reagent Blank 5 Press On to highlight the Reagent Blank function 6 The concentration shown on the key is the measured value of the reagent blank To use this value for more analyses of this parameter press OK To enter a previously recorded reagent blank value press the key and use the alphanumeric keypad 7 Press OK Note The Reagent Blank function is deactivated when the measurement program is left To use the same blank value later for other tests using the same reagent lot enter the value per step Note The results calculated using the reagent blank value must lie within the limits of the measuring range of the test method Note The reagent blank icon is shown in the result display see arrow when the function is active Standard Operations 5 4 9 Analysis of samples 10 Aluminum Alumin VIS 522 nm 1 Press Stored Programs and select a program 0 0 0 0 2 Insert the blank cuvette cell into the cuvette sample cell holder L 3 Press Zero 18 SEP 2006 15 18 52 Options 10 Aluminum Alumin VISX 522 nm E 4 Remove blank cuvette cell and insert sample cuvette cell into the cell compartment 5 Press Read The result will be displayed 6 For data storage see section 5 3 2 on page 38 18 SEP 2006 15 19 01 yo
115. rammed and press Edit 5 To finish programming press Store and then Cancel to return to the Main Menu 6 Press Start to run the test with the new data 5 5 7 Upgrade of the instrument software systr Abanke Instrument Update Please insert an Instrument Update Module into a USB Port or connect to a computer that is running the update program saa d 58 To obtain the software for the update from the Internet at www hach com 1 Go to http www hach com 2 Onthe DR 5000 product page click Lab System Software Software Update Downloads under the heading Downloads 3 Locate the appropriate download and follow the prompts for saving the file s to the USB memory stick 4 Onthe DR 5000 press Instrument Update in the System Checks menu 5 Connect the USB stick to the USB interface on the DR 5000 section 3 3 on page 14 Press OK The link is established automatically and the software is updated Standard Operations 6 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed 59 Standard Operations 60 Section6 Advanced Operations 6 1 User Programs User Programs User programs provide the opportunity to complete made to measure analysis The User Programs database is empty when the instrument leaves the factory and is used to accommodate programs created by users specific needs
116. repair replacement or refund of purchase price as stated above are the exclusive remedies for the breach of this warranty On the basis of strict liability or under any other legal theory in no event shall Hach Company be liable for any incidental or consequential damages of any kind for breach of warranty or negligence 133 Limited Warranty 134 Index A Absorbance Check rds 111 Absorbance Formula ooncnnncncncnnnncocicccnononononononos 90 91 Alphanumeric keypad coccccccccnccnnocconcncconccnccccnncnnannns 21 A e ete et es 25 B Barcode Programs sssssussss 51 52 53 latir indidit 53 Basic data oos bo gie a riae 42 C Calibration formula esses 75 Calibration Settings sssssess 66 Chemical Form oocccccnnnnncnnncnnnnnnnnonononononenonos 43 47 53 Chemical form essere 51 52 Cleaning DISplay 4o bea s aaae 121 Spectrophotometer sssssssss 121 Concentration Factor 87 88 90 92 Concentration Resolution 87 88 90 92 Continuous Reading sssssssss 43 45 Cursor Mode innen eee dies 94 96 Cuvettes cells 2 ted er eet 121 D Data delete tM e 35 37 recall E AE T a e um 35 A ens Het a a eraa aiia 35 Co RES 35 Data Storage dE 38 Date ii Eu E 25 Date and Time ne uas 25 Dilution Factor ooonnnnnnnncicococonononononcncnononononos 4
117. rn to the scan mode Integral The Integral applies to the whole wavelength range of the scan 1 Press Integral Off in the Options menu 2 Highlight On to show the Integral To find the integral of other wavelength ranges change the wavelength range and scan again 3 Press OK to confirm Optic Scale amp Units Units Abs Scale Auto C Manual 0 300 Abs uz 3 500 Abs Cancel OK len 6 6 2 Wavelength scan reading Wavelength Scan 400 Zeroing Main Menu Advanced Operations 4 Press Return to return to the scan mode Note The Integral is shown instead of the date on the display Note For the next scan measurement the setting for the Integral will be On Scale amp units 1 Press Scale amp Units 2 Highlight the required units Abs or T 3 Highlight Auto or Manual scaling on the graph s y axis Note If manual scaling is selected use the alphanumeric keypad to set the limits Y min and Ymax The graph is adjusted to display only the values in the selected range If automatic scaling is selected the instrument sets the limits automatically so that the total range can be displayed 4 Press OK 5 Press Return to return to the scan mode After the scanning parameters have been selected the baseline must be scanned Changing any of the scanning parameters requires a new baseline scan When the baseline has been scanned the instrument is ready to sc
118. ry was made during the data update and is not recognized via the barcode of the cuvette sample vial e g an DB non incorrect measuring range another option is available to check z and if necessary to update the test data el 1 2 Instrument Return Setup 3 4 The instrument need to be at the Main Menu Insert the blank or sample cuvette vial depending on the working procedure into the cell compartment 1 and close the cell compartment Press Options gt More gt Edit A data overview is displayed including the specifications of the test that is to be revised Compare the displayed data with the data in the working procedure and edit the displayed data in accordance with the working procedure 57 Standard Operations 5 5 6 3 Program a new test Program not available Please update program data Program 341 Name Cancel 1 The instrument needs to be at the Main Menu 2 Insert the sample cuvette vial or blank depending on the working procedure into the cell compartment 1 and close the cell compartment 3 After Program not available a data overview is displayed including the specifications of the test that is to be revised The working procedures of the corresponding test contains the new data Wavelength Factors Measurement Ranges Conversion Factor for chemical Form etc 4 Highlight the corresponding line including the specifications of the test that is to be prog
119. se There are two ways to recall sent data to a USB memory stick printer or PC with Hach Data Trans Recall Data Wavelength Scan Option 1 15 SEP 06 14 20 33 Bur PESE D 1 Press Recall Data in the Main Menu and then Wavelength 09 19 40 400 EBD hr dc OUI Scan or Time Course 09 26 44 400 600 nm A 001 nm _ 2 Press Options and then the PC amp Printer icon to send the 09 29 14 Delete data to a USB memory stick to a printer or to a PC with Hach 400 600 nm 001 nm Data Trans 10 01 32 400 600 nm 001 nm 39 Standard Operations Send Data Iv Graph M Table E Cancel OK Options Cursor Mode cH Send Track Data Integral 40 Return Instrument Setup e When a printer is connected select how to send the data to the printer graph table or both graph and table e When a USB memory stick is connected the files will be automatically sent as CSV files Comma Separated Value to a file WLData Wavelength Scan Data or TCData Time Course Data to the USB memory stick The file name will be formatted as ScanData_X csv Wavelength Scan Data or TCData X csv Time Course Data X number of scans 1 20 For further processing use a spreadsheet program Note The advice Data already exist Overwrite appears when the files were already stored Press OK to overwrite the stored data Option 2 1 Press Wavelength Scan or Time Course and then Options gt
120. st or sample specific settings rm Send Reading Mode r Data Single 0 053 7 PO 4 P Dilution din pemi AE Factor Off Trans Save as User Program 21 SEP 2006 14 09 05 z bi Ret Instrument ero Options eum Setup Table 5 Barcode program options Options Description More For further Options With the Store On setting all measurement data are stored automatically With the Store Off Stone Oron setting no measurement data are stored Abs Trans To switch to 96 transmittance or absorbance readings 52 349 Phosphate 0 05 Standard Operations Options A Send Reading Mode Data Single mg l 3 Dilution Chemical Form PO4 P abe wb Factor Off 4 PO P Trans 3 Save as User Program 21 SEP 2006 14 09 05 b d Zero Read Return iia nt Table 5 Barcode program options continued Options Send Data icon Send Data Timer icon Dilution Factor Off On Description To send data to a printer computer or USB memory stick Type A This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program A corrective dilution factor can be entered in order to take account of certain properties The number entered at the dilution
121. the display depends on the definition of the measurement process and the formulas Input of the numerical values of the wavelengths factors constants etc Timer 1 Timer 2 Timer 3 Timer 4 This function can be used to enter abbreviations and defined times for up to four timers Highlight the appropriate line and press Edit The timers are activated or deactivated with the control boxes on the left of the display In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered Program 341 Measurement Process Cancel 54 Enter the Name Check the working procedure in advance to determine whether this point needs to be changed 1 Highlight the line containing Name and press Edit Use the alphanumeric keypad to enter the name indicated in the working procedure 2 Press OK to confirm oat Measurement Process lt aaa Formula C2 mg l NO0z N C3 mg l NOz Standard Operations Enter the Version 1 Highlight the line containing Version and press Edit Use the alphanumeric keypad to enter the name indicated in the working procedure 2 Press OK to confirm Enter the Measurement Process For detailed information on entering the measurement process see section 6 1 2 on page 72 Check the working procedure in advance to determine whether this point needs to
122. to Permanent Off 5 5 3 Select the chemical evaluation form The chemical form of the test result of a number of parameters can be selected individually In the result display press the unit e g mg L or the chemical representation of the evaluation form e g PO 3 P A list of possible evaluation forms is displayed from which the required form can be selected Press OK to confirm Another way of changing the standard setting is 51 Standard Operations Optic Chemical Form 1 Inthe result display press Options gt More gt Chemical Form PO P A list of available evaluation forms appears PO4 7 P total PO 2 Select the required chemical form and press OK to confirm PO 4 total P20 Note The selected chemical form is displayed but does not become P0 total the default To change the default see section 5 5 3 1 j i canal Default oK lev 5 5 3 1 Change of the default setting of the chemical form 1 Insert the blank or sample cuvette vial depending on the working procedure into the cell compartment and close the cell compartment 2 In the result display press Options More Chemical Form 3 Alist of available evaluation forms appear Select the new default setting 4 Press Save as Default The current result and all further measurements will be displayed in the new chemical form 5 5 4 Basic test specific and sample specific data settings Press Options to change te
123. to a PC with installation of the HACH Data Trans Software These USB ports can be used to connect a printer USB memory stick a barcode scanner or a keyboard USB Type B USB Type A 1 Press PC amp Printer in the Instrument Setup A list with information about the connections opens For reasons of compatibility the printer language must be HP PCL 3 2 Press Printer 3 Press Setup to display the Printer Setup screen 29 Standard Operations Instr ont Latin Printer Setup Printer Setup t Resolution TEPEE i e Resolution Print quality 100 dpi LETTER E e Paper Paper size Note Ifan optional Thermal Printer is connected the function Auto Send on off is available 4 Select Auto Send On to send all measured data automatically f to the Thermal printer Resolution Paper 100 dpi LETTER Note The option Auto Send is not available for any other printer e g ink Auto Send jet printer On cm ox 5 Press Resolution to select the print quality Select between e 100 dpi e 150 dpi and e 300 dpi 6 Press OK to confirm Note Press OK again to return to the PC amp Printer menu 30 Standard Operations 5 2 6 2 Print data Recall Data 11 SEP 06 14 16 00 1004 o mg l DCO 15 SEP 06 09 15 04 wW 05 02 3 93 mg l PO47 P 15 3 59 9 A Sending Data 15 SEP 06 10 08 00 ww 0O5
124. upper and lower case letters Symbols Punctuation symbols and numerical sub and superscripts may be entered 123 Numeric For entering regular numbers CE Clear Entry Clear the entry Left Arrow Backspace Moves back one position This deletes the character previously entered in the new position Right Arrow Adv nc Moves to the next space in an entry when two adjacent characters occur on the same key 21 Standard Operations 5 1 3 Main Menu Main Menu Stored Programs pane dbi tania Single Wavelength Multi Wavelength A variety of modes may be selected from the Main Menu The following table briefly describes each menu option e Prin System Checks Option Instrument Setup Table 2 Main Menu options Function Stored Programs HACH LANGE Programs Stored programs are pre programmed methods that make use of HACH reagents and LANGE cuvette tests and pipette tests The DR 5000 Procedures Manual contains illustrated step by step procedures for analyses using HACH programs The working procedures for LANGE tests are included in the test packs User Programs User programs make made to measure analysis possible Users can program methods they have developed themselves Existing HACH and LANGE methods can be stored as user programs The LANGE tests can then be modified to suit the user s requirements Favorite Programs List of methods tests created by the user to suit h
125. ure is repeated for each of them Note The units and chemical forms used for the sample are used for the standard solutions Be careful to ensure that to use the correct units for subsequent entries 83 Advanced Operations 10 Aluminum Alumin Abs Trans 22 SEP 2006 14 17 32 4 Reading Mode Single Dilution Standard Factor Off Additions LA Standard Chemical Form al Adjust Off B Reagent Blank Save as Off User Program Instrument Setup Standard Additions Standard Concentration 50 00 mg L AI 5 Sample volume 50 0 mL Spike Volumes mL 0 0 0 1 0 2 0 3 Cancel Edit OK en 84 1 Peak volume sample volume methods Select Stored Programs in the main menu Select the required program Press Start Analyze a sample without added standard solution in accordance with the instructions in the Procedures Manual When the measurement is complete leave the sample cuvette cell in the cuvette sample cell holder Press Options gt More gt Standard Addition An overview of the data of the standard addition procedure is displayed Press OK to accept the standard values for standard concentration sample volume total and standard addition volume Press Edit to change any of these values Advanced Operations Standard Additions 6 Press the key of the value to change it Use the alphanumeric keypad to change the value Press OK to confirm Standard Concentration
126. ut press Read standard setting see section 6 4 2 on page 89 Continuous Reading Mode After the zero measurement all readings are displayed automatically and continuously see section 6 4 3 on page 89 Reading Mode Save as User Program To store the selected parameters as a User Program see section 6 1 on page 61 Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 35 Instrument Setup Basic data of the instrument see section 5 2 on page 23 Optic p Concentration Factor Concentration factor 1 Press Concentration Factor Off in the Options menu Press On to highlight this feature 2 Press the Factor key and use the alphanumeric keypad to enter the factor by which absorbance readings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit 3 Press OK to confirm Optic Concentration Resolution Concentration resolution 1 Press Concentration Resolution in the Options menu 2 Select the resolution and press OK to confirm 88 Reading Mode Single Reading C Continuous Readings Advanced Operations Reading mode 1 To highlight the required mode start by pressing Reading mode 2 Selectthe required mode then press OK then Return to return to the result display 6 4 2 Take single wavelength measurements single reading
127. val can be longer 1 Remove any cuvettes and cuvette sample cell adapter from the cell compartment 2 Turn the instrument off 3 Unplug the power cord 4 Lift the instrument and check the color of the filter mat Replace the filter mat ifis dark gray or black 5 Carefully turn the instrument over and place it on a soft surface 6 Use a screwdriver standard or cross head to open the filter grid Figure 9 on page 126 item 1 7 Lift the filter grid Figure 10 on page 126 item 1 8 Remove the old air filter pad and replace it with a new air filter pad Figure 10 item 2 9 Screw the grid back in place 10 Carefully stand the instrument upright 11 Plug the instrument in 125 Maintenance Figure 9 Base of DR 5000 with filter grid Phillips screw 3 Filter grid 1 2 Filter pad Figure 10 Change of filter pad Filter pad 1 126 Section 8 Troubleshooting Problem Display screen Likely Cause Action Absorbance gt 3 5 The measured absorbance exceeds 3 5 Dilute the sample and repeat the measurement Concentration too high Calculated concentration is higher than 999999 Dilute the sample and repeat the measurement Error Barcode controlnumber Please update program data Deviation to the stored data Data updating Error Program not available Please update program data Barcode cuvette test missing Data updating Error

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