Gelfree 8100 Protein Fractionation System
Contents
1. Instrument Placement The GELFREE 8100 Fractionation Station includes a high voltage power supply and electronics that are sensitive to moisture extreme temperature and electromagnetic interference Heat generated when using the instrument is dissipated using a series of fans in the rear of the instrument Proper airflow must be ensured to avoid overheating of the instrument during operation As such the instrument should be placed on a level table or laboratory bench removed from routine handling of liquid reagents The minimum recommended space is 24 W x 24 D x 18 H allowing for 8 of unobstructed space behind the instrument Figure 1 Proper Instrument Placement 2013 Expedeon Inc All Rights Reserved Ss EXDE edeo GELFREE 8100 Fractionation System 7 of 30 protein JEO amp Nn Description of Parts GELFREE 8100 Fractionation Station The GELFREE 8100 Fractionation Station is an independently controllable eight channel electrophoretic power supply designed specifically for use with GELFREE 8100 Cartridge Kits It features a touch screen display power supply capable of delivering constant voltage 20 300 V for pre programmed time intervals and retractable electrode arrays The primary instrument components referred to in this manual are labeled below Instrument Enclosure Touch Screen Display Figure 2 GELFREE 8100 Fractionation Station 2013 Expedeon Inc All Rights Reserved2. means a use in manufacturing b inclusion of the Product s as part of another product c use of the Product s to provide any service for compensation d use for ex vivo or in vivo therapeutic diagnostics forensic prophylactic or other unauthorized commercial purpose e use in foods drugs devices or cosmetics of any kind or for consumption by or use in connection with or administration or application to humans or animals f resale of the Product s whether or not for research use and g any use other than research use Any Buyer who does not agree with these terms can return the Product s at their own cost and will receive a full refund excluding shipping tax and handling fees For information on purchasing a license to the Product s for purposes other than research use contactus at info expedeon com 2013 Expedeon Inc All Rights Reserved E expe eon 8100 Fractionation System 30 of 30 Corporate Headquarters www Expedeon com expedeon Expedeon Ltd Unit 1A Button End Harston Cambridgeshire CB22 7GX Tel 44 0 1223 873364 Fax 44 0 1223 873371 Email info expedeon com Expedeon Inc 11211 Sorrento Valley Road Suite C San Diego 92121 Tel 858 457 7978 Fax 858 457 7939 Email info expedeon com Expedeon Asia Pte Ltd 11 Gerald Crescent Singapore 797726 Tel 65 9711 7429 Fax 656438 5085 Email info expedeon com
3. Cavort Channel Method C 8v 14 6 mA 35V 14 7 mA 5v 14 8 mA Time Elapsed sv 14 8 mA 00 00 40 85v 14 9 mA 2 3 4 5 85V 14 9 mA 6 7 s sv 14 8 mA Selected Method 1 Step 1 of 12 85 V 1 min Lid State Figure 7 The GELFREE 8100 Fractionation Station Main Screen 2013 Expedeon Inc All Rights Reserved S Jexpedeonere 8100 Fractionation System 13 of 30 protein discovery amp analysis Operating Procedures cont Method Development Editing Note Be sure to refer to the Cartridge Kit Reference Card provided with each Cartridge Kit when setting up and running the fractionation method To create a new method follow these steps 1 On the Main Screen touch the Method button displayed in the upper right hand corner Figure 7 This will take you to the Methods Screen Figure 8 Methodt Step Voltage Time Min Sec 85 V 05 00 85 V 11 00 Retrieve 85 V 01 00 85 V 01 00 Save 85 V 02 00 85 V 02 00 85 V 02 00 85 V 02 00 85 V 05 00 85 V 05 00 Prev Figure 8 The GELFREE 8100 Fractionation Station Methods Screen y Apply 2 3 4 5 6 7 8 9 y O 2 Touch the Retrieve button This will bring up a dialogue screen Figure 9 requesting a method number 3 Begin by pressing the C button on the number pad to clear the existing input Next enter a method number that has not been used and press OK This will lead to the screen shown in F
4. Jexpedeon GELFREE 8100 Fractionation System 8 of 30 protein discovery amp analysis Description of Parts cont Cartridge Positioning Key Figure 3 GELFREE 8100 Fractionation Station 2013 Expedeon Inc All Rights Reserved Ss lt EXDE ede on GELFREE 8100 Fractionation System 9 of 30 protein JGO amp analysis Description of Parts cont GELFREE 8100 Cartridge Kit The GELFREE 8100 Cartridge is a patented single use cartridge designed for insertion into the GELFREE 8100 Fractionation Station The cartridge is supplied with storage buffer and specially designed precast gels ensuring maximum separation reproducibility The primary cartridge components are labeled below Sample Loading Sample Collection Chambers Chambers AANA aa Cathode Buffer Anode Buffer Reservoirs C Reservoirs Figure 4 GELFREE 8100 Fractionation Cartridge Included with each Cartridge Kit is the Running Buffer 500 mL and 5x Sample Buffer 1 9 mL which comprises all the buffer necessary to run all eight channels in the cartridge Note DO NOT REFRIGERATE the cartridge The cartridge must be stored at room temperature 20 25 C at all times 2013 Expedeon Inc All Rights Reserved ex edeon 8100 Fractionation System 10 of 30 protein discovery amp analysis Operating Procedure
5. Once running the Start button becomes the Pause button which allows the user to pause the run 5 To completely stop an experiment once it has been started press the Abort button on the Main Screen A confirmation screen will appear Figure 13 To abort touch Yes or touch No to return to the Main Screen 2013 Expedeon Inc All Rights Reserved jexped GELFREE 8100 Fractionation System 18 of 30 protein JCO amp Nn oaral Procedures cont 2 Pause Cabor Channel Method C 85V 14 6 mA Abort eee 85V 148 mA 85v 14 9 mA 85v 14 8 mA 1 2 3 4 5 6 7 8 Selected Method 2 Step 1 of 12 85 V 5 min Lid State O Figure 13 The GELFREE 8100 Fractionation Station Abort Experiment Monitoring a Method 1 Channel Status The circles shown in the box on the left side of the Main Screen indicate the status of each channel A green circle indicates that the channel is active The applied voltages and currents are displayed to the right of the channel status circles When the method is paused the circles will turn yellow If the method fails to start or fails during the run the circles will turn red When the circles are either yellow or red the voltage readout is zero 2 Time Monitor By touching the button above the digital time counter the user can toggle between Time Elapsed Time Remaining or Time To Pause The progress bar provides
6. expedeon GELFREE 8100 Fractionation System Molecular weight fractionation with liquid phase recovery cc expedeon protein discovery amp analysis GELFREE 8100 Fractionation System 2 of 30 Table of Contents General Information Introduction Types of Products Components Specifications Safety Information Unpacking and Placement Unpacking and Inspection of Parts Instrument Placement Description of Parts GELFREE 8100 Fractionation Station GELFREE 8100 Cartridge Kit Operating Procedures Installation and Power Up Loading the Cartridge amp Electrode Placement Operating the Software Running the Cartridge Maintenance and Technical Service User Notifications Bibliography Limited Use License 2013 Expedeon Inc All Rights Reserved Oo N N A Oo Oo oO A U W U W N N N N N FP Be FF F amp F o N Oo U O N O O OO Jexped on GELFREE 8100 Fractionation System 3 of 30 protein JCO amp analysis onora Information Introduction The GELFREE 8100 Fractionation System enables preparative scale fractionation of analytes according to electrophoretic mobility The GELFREE Gel Eluted Liquid FRaction Entrapment Electrophoresis technique provides all of the advantages of 1D gel electrophoresis with the additional benefits of increased loading capacity and high yield liquid phase recovery The proprietary GELFREE design allows for concurrent fractionation of up to 8 samples The system is c
7. 2 3 4 5 6 7 8 9 Figure 11 The GELFREE 8100 Fractionation Station Develop Edit New Method 2013 Expedeon Inc All Rights Reserved ex edeon GELFREE 8100 Fractionation System 16 of 30 protein discovery amp analysis Operating Procedures cont 5 Review the voltage time profiles for each step in the new method Press Save on the Method Screen Figure 10 A pop up box will appear with a default method number of 1 Clear or type over the method number to give the newly created method a different number 6 Next press Apply on the same screen to go back to main screen Figure 7 To edit an existing method follow the steps outlined above with the following exception Rather than using a number for a new method in step 3 enter the number of an existing method Edit the method as described above and then save the method Important It is critical that every method developed for use with the GELFREE 8100 Fractionation Station incorporate a first step to allow the user to add an additional 2 mL of Running Buffer to the Cathode Buffer Reservoir bringing the total volume to 8 mL and to thoroughly rinse the Sample Loading Chamber For standard methods the first step parameters are shown on the Quick Reference Card provided with each Cartridge Kit For a custom method the first step should be applied once the dye front is visible in the gel tube 16 minutes at 50 V 8 minutes at 100 V Executi
8. with GMA 3 R or equivalent 3A 250VAC fast blow fuse Max Power 450 VA Temperature Limits Storage 10 60 C 30 80 relative humidity Operating 22 C 30 80 relative humidity Ingress Prot Rating IPXo GELFREE 8100 Cartridge Specifications Cartridge Size 12 5 cm x 8 5cm Gel Size L 2 5cm OD 8mm Sample Volume 250 uL Reservoir Volume 9 5 mL Temperature Limits Storage 20 25 C 30 80 relative humidity Operating 22 C 30 80 relative humidity GELFREE 8100 Cartridge Buffer Specifications Running Buffer 500 mL Sample Buffer 1 9 mL Temperature Limits Storage 20 25 C 30 80 relative humidity Operating 22 C 30 80 relative humidity 2013 Expedeon Inc All Rights Reserved ex edeo f CELFREE 8100 Fractionation System 5 of 30 protein discovery amp analysis Safety Information During operation the GELFREE 8100 Fractionation Station lid must always remain closed to ensure the safety of the operator This product is safe to use when operated in accordance with this instruction manual If this unit is used or modified in a manner not specified in this manual then protection afforded by the unit will be impaired Alteration of this unit will Void the warranty Create potential safety hazards Protein Discovery is not responsible for any injury or damage caused by use of this device when operated for purposes for which it is not intended All repairs and services should be performed b
9. 6 indicate the direction of the applied force when raising and lowering the electrodes Do not force the electrodes into position this can permanently damage the mechanism Very little resistance should be met when raising and lowering the electrodes into position 2013 Expedeon Inc All Rights Reserved expedeon GELFREE 8100 Fractionation System 11 of 30 protein discovery amp analysis Operating Procedures cont Figure 5 GELFREE 8100 Fractionation Station with cartridge and retracted electrodes Follow arrows to lower electrodes Figure 6 GELFREE 8100 Fractionation Station with cartridge and lowered electrodes Follow arrows to lift electrodes 2013 Expedeon Inc All Rights Reserved expedeon GELFREE 8100 Fractionation System 12 of 30 protein discovery amp analysis Operating Procedures cont Operating the Software Introduction The GELFREE 8100 Fractionation Station is controlled by an onboard computer with a touch screen interface The primary operating modes are the following K2 Method Development Editing Method Execution e Method Monitoring L2 SS hS L2 Once the device is powered up the central navigation and operating screen will be displayed on the touch screen interface The Main Screen includes the key features necessary to navigate the control software and develop edit execute and monitor separation methods Figure 7 2 Pause
10. a visual indication of all time metrics 2013 Expedeon Inc All Rights Reserved a Ss expedeon GELFREE 8100 Fractionation System 19 of 30 protein discovery amp analysis Operating Procedures cont 3 Active Method At the bottom left corner of the Main Screen the method that is currently selected is shown 4 Method Status At the bottom center of the Main Screen the current step that is active is shown e g Step 1 of 12 50 V 16 min 5 Lid Status At the bottom right corner of the Main Screen a green circle indicates that the lid is closed ready safe to operate and a red circle indicates that the lid is open will not operate 6 Software Release Information Touch the PDI icon found in the top left of the screen to view the current software version running the GELFREE 8100 Fractionation Station 2013 Expedeon Inc All Rights Reserved E expede on GELFREE 8100 Fractionation System 20 of 30 protein JEO amp analysis Operating Procedures cont Running the Cartridge Introduction It is imperative that the steps below are followed in sequence 1 Prepare the sample s and create or modify existing method 2 Prepare the cartridge and load the sample s 3 Start the run 4 Collect the fractions Preparing the Sample s Important Notice The presence of low molecular weight contaminants such as detergents urea and excessive salts will negatively impact the fractionati
11. can be left in place during this step Add 2 mL Running Buffer to the Cathode Buffer Reservoir of each of the active channels and resume the Method Note At this point the sample will have migrated into the gel and a band of dye will be visible near the left end of the gel Note Upon resuming the method check for proper operation as in Step 2 above 4 The Station will pause for collection of fraction 1 at the second pre defined pause interval At that point use a single or multi channel pipette to transfer the contents of the Sample Collection Chambers to the first column of a 96 well plate Then discard the tips Note To maximize recovery pipette up and down twice before removing the sample 5 Using fresh pipette tips wash the Sample Collection Chambers twice with 200 uL fresh Running Buffer Pipette up and down at least twice before removing and discarding the wash buffer Fill the Sample Collection Chambers with 150 uL of fresh Running Buffer and resume the Method 6 At each pre programmed pause interval repeat the collection wash and fill steps 4 5 placing fraction 2 into column 2 of the 96 well plate fraction 3 into column 3 and so on 2013 Expedeon Inc All Rights Reserved a Jexpedeo f Iecere 8100 Fractionation System 24 of 30 protein discovery amp analysis Operating Procedures cont T To ensure proper buffering capacity Running Buffer must be replaced in the Anode and Cathode Buff
12. d for Buyer to use the Product s for research use and such transfer is not for any Non Licensed Use and provided that such collaborator recipient agrees in writing a not to transfer such materials to any third party and b to use such transferred information or materials solely for research purposes and not for any Non Licensed Use Except as expressly permitted the Buyer is not permitted to sell or otherwise transfer the Product s the Product s components and or derivatives or materials made using or incorporating the Product s to a third party Expedeon will not assert a claim against the Buyer for infringement of the Expedeon Technology based upon the manufacture use or sale of a therapeutic diagnostic vaccine or prophylactic product developed by the Buyer in research in which a Expedeon Product was employed provided that the Expedeon Product is not part of such other product and the Expedeon Product is not used in the manufacture of such other product In this Limited Use License Expedeon Technology means all patents pending patents and know how owned by Expedeon that cover or relate to the Product s the identification extraction and or production of the Product s and or the use of and or applications for the Product s 2013 Expedeon Inc All Rights Reserved Jexpede on GELFREE 8100 Fractionation System 29 of 30 protein JEO amp analysis Limited Use License cont Non Licensed Use
13. er Reservoirs after approximately 30 minutes of cumulative run time at 100V or 60 minutes of cumulative run time at 50 V according to the following procedure a Remove the cartridge from the instrument and discard the buffer b Fill each Anode Buffer Reservoir with 8 mL Running Buffer c Fill each Cathode Buffer Reservoir with 8 mL Running Buffer d Fill the Sample Loading Chambers with 150 pL Running Buffer e Fill the Sample Collection Chambers with 150 pL Running Buffer f Return the cartridge to the instrument and resume the Method 8 Repeat the Sample Collection Chamber collection wash and fill steps for each subsequent fraction 9 Once all of the Method s steps have been completed the display screen will indicate that the experiment has ended Collect the final fraction and enjoy the benefits of reproducible high yield liquid phase fractionation in your protein research 2013 Expedeon Inc All Rights Reserved ex edeon GELFREE 8100 Fractionation System 25 of 30 protein discovery amp analysis Maintenance and Technical Service Cleaning Specifications General maintenance for the GELFREE 8100 Fractionation System includes wiping the unit with a soft dry or slightly dampened cloth Do not handle or clean electrodes Do not use solvents or detergents info Replacement Parts Please contact Expedeon Inc for any replacement parts for the GELFREE 8100 Fractionation System The use of par
14. fer from the reservoirs associated with the active channels 2 Add 8 0 mL of Running Buffer to the Anode Buffer Reservoir for all active channels 3 After rinsing out the Storage Buffer with two 200 uL aliquots of Running Buffer add 150 uL of Running Buffer to the Sample Collection Chambers for all active channels 4 Add 6 0 mL of GELFREE Running Buffer to the Cathode Buffer Reservoirs for all active channels Important Notice Take care to avoid introducing and trapping bubbles in the Cathode and Buffer Reservoirs as they will block electrical current and impact elution time reproducibility To dislodge bubbles potentially trapped against the membrane in the Buffer Reservoirs simply flush the membrane with Running Buffer using a pipette Note Overfilling the reservoir at this point may result in irregular sample migration and separation 2013 Expedeon Inc All Rights Reserved Ss lt EXDE edeo f GELFREE 8100 Fractionation System 22 of 30 protein JEO amp Nn Operating Procedures cont 5 Using a fine tipped transfer or multi channel pipettor remove and discard any buffer that has flowed into the Sample Loading Chambers from the Cathode Buffer Reservoirs for the channels being loaded Immediately load 150 uL of the prepared sample into the Sample Loading Chamber of the corresponding channels Be careful to avoid introducing bubbles into the Sample Loading Chambers Note Buffer passes freely through the me
15. igure 10 Touch the step number to be modified 2013 Expedeon Inc All Rights Reserved E expedeon GELFREE 8100 Fractionation System 14 of 30 protein discovery amp analysis Operating Procedures cont Method 1 Step Voltage Time Min Sec y O CON DM UI BW N Figure 9 The GELFREE 8100 Fractionation Station Retrieve Method Method 2 Step Voltage Time Min Sec OV 00 00 OV 00 00 OV 00 00 OV 00 00 OV 00 00 OV 00 00 OV 00 00 OV 00 00 OV 00 00 OV 00 00 Prev Figure 10 The GELFREE 8100 Fractionation Station Define New Method y 2 3 4 5 6 7 8 9 y O 2013 Expedeon Inc All Rights Reserved Jexped f GELFREE 8100 Fractionation System 15 of 30 protein JEO amp Nn oara Procedures cont 4 This leads to the input screen shown in Figure 11 The green cell indicates an active cell where a value for voltage or time can be added or modified by following these simple steps a Cancel the value in the cell using the C on the number pad b Enter the desired value using the keypad c Touch the inactive cell to activate it Then repeat steps a and b d The Next and Previous buttons allow the user to edit the next step or the previous step respectively A maximum of 30 steps may be included in any single method e When editing is complete touch the OK button Method 2 Step Voltage Time Min Sec Step 1 OQ Time min 1 alojo m e
16. mbrane separating the Cathode Buffer Reservoir and the Sample Loading Chamber Be sure to load the sample immediately after removing Running Buffer in the Sample Loading Chamber and immediately before running the cartridge Performing the Run and Collecting the Fractions 1 Place the cartridge in the instrument lower the electrodes and close the lid 2 Start the method by touching Start on the Main Screen Note Proper operation is indicated by a green circle next to each channel number on the Main Screen Note If operation fails check these items v Lid properly closed indicated by green circle at bottom right of screen v Correct method selected indicated at bottom left of screen Correct voltage and current displayed for all channels as set by user Buffer in all Cathode and Anode Buffer Reservoirs All electrodes contacting buffer Buffer in all Sample Collection Chambers No bubbles around the membranes restricting current flow i SSS 2013 Expedeon Inc All Rights Reserved E expede on GELFREE 8100 Fractionation System 23 of 30 protein JEO amp analysis Operating Procedures cont 3 At the end of the first step as specified on the Quick Reference Card for the specific Cartridge Kit used the instrument will pause for rinsing of the Sample Loading Chambers with Running Buffer and addition of an extra 2 ml of Running Buffer in the Cathode Buffer Reservoirs The electrodes
17. ng a Method 1 Retrieve a defined method by pressing the Method button on the Main Screen as previously described Once the method has been retrieved press Apply to apply the method and return to the Main Screen 2 Select the channels to run in the cartridge by pressing the Channel button on the Main Screen This will bring up the Channel Screen Figure 12 Select the combination of channels to run during the experiment by pressing the corresponding channel button e g Channel 1 or press 2013 Expedeon Inc All Rights Reserved Jexpedeon GELFREE 8100 Fractionation System 17 of 30 protein discovery amp analysis Operating Procedures cont Set All Channels to apply the method to all eight channels Once the desired combination of channels has been selected press Done to return to the Main Screen Channel 1 Set All Ch Channel 2 a Channel 3 Clear Channels Channel 4 Channel 5 Channel 6 Channel 7 Channel 8 chaen Q C Chamei2 _ chames chaeis _ Q _Channets ___ chameis___ chaer chames Figure 12 The GELFREE 8100 Fractionation Station Channel screen 3 Press the polarity button on the Main Screen to set the polarity of the field For all experiments using SDS the polarity should be Negative 4 Once the method has been applied and the channels selected press the Start button on the Main Screen to begin a run
18. omprised of the GELFREE 8100 Fractionation Station a bench top instrument and single use GELFREE 8100 Cartridge Kits The Station is a programmable power supply that accurately applies electrical potential stopping at user specified intervals for fraction collection A touch screen graphical user interface provides easy and simple navigation while creating executing or monitoring fractionation methods Precast gel cartridges ensure the highest possible reproducibility while providing quick and easy setup Types of Products This manual is supplied for the following products Product Catalog no GELFREE 8100 Fractionation System 48100 42102 42103 GELFREE 8100 Cartridge Kit 42104 42105 Components The components included with the different products are as follows GELFREE 8100 Fractionation GELFREE 8100 Fractionation System Station Power Cord User Manual GELFREE 8100 Cartridge Kit GELFREE 8100 Cartridge GELFREE 8100 Running Buffer GELFREE 8100 Sample Buffer 2013 Expedeon Inc All Rights Reserved a d GELFREE 8100 Fractionation System 4 of 30 E expe eon ractionation System 4 o protein discovery amp analysis Specifications GELFREE 8100 Fractionation Station Specifications Dimensions 33 cm W x 14 cm H x 33 cm D Clearance amp Ventilation 38 cm vertical clearance for cartridge loading 20 cm back clearance for airflow Weight 4 5kg 10 Ibs Power 100 240 VAC 50 60 Hz replace fuse
19. on results Before you start be sure to fully desalt and remove any known contaminants If your sample has particulates we highly recommend passing the sample through a 0 8 um syringe filter to remove any loose particulates prior to further preparation Once the sample is ready for preparation please follow these steps 1 In a 500 uL vial combine the following a Upto 112 uL Sample b 30wuL Sample Buffer 5X c 8puL 1M DTT optional not provided in the kit d xpylLH 0 e 150 uL Note If the sample volume is less than 112 uL add 18MO H20 to bring the final volume to 150 uL 2013 Expedeon Inc All Rights Reserved expedeon GELFREE 8100 Fractionation System 21 of 30 protein discovery amp analysis Operating Procedures cont 2 Heat the vial for 10 minutes at 50 C Note Overheating the sample will cause protein degradation 3 Allow sample to cool to room temperature 22 C Note Changes in sample buffer cartridge or room temperature will cause changes in elution time For best results run the system with all solutions at 22 C Preparing the Cartridge and Loading the Sample 1 Remove the cartridge from the foil package and remove the plate sealer If all 8 channels in the cartridge will be utilized in one experiment dump out the storage buffer found in all compartments If only some of the 8 channels will be utilized use a Pasteur or transfer pipette to remove only the Storage Buf
20. s Installation and Power Up To power on the instrument insert the provided power cord into the socket on the back of the instrument move the instrument to its final position and plug the cord into a suitable power supply 100 240 VAC 50 60 Hz Power on the unit using the switch located on the back of the instrument above the power cord During the power up procedure the Protein Discovery logo will be displayed on the touch screen Once powered up the main control screen for the instrument will be displayed Verify proper operation of the high voltage power interlock by opening and closing the lid of the instrument In the lower right corner of the main screen the Lid State indicator will switch from green closed to red open to indicate engagement of the interlock Loading the Cartridge and Electrode Placement Opening the lid allows access to the cartridge compartment for loading and unloading the cartridge and for collecting fractions during the experiment The cartridge is loaded such that the well numbers imprinted on the cartridge can be read by the operator and the arrows imprinted on the cartridge point to the right The cartridge positioning key Figure 3 prevents the user from loading the cartridge in the wrong orientation The loaded cartridge should rest fully on the bottom of the cartridge compartment With the cartridge in place the electrode arrays are lowered in place by the user The arrows in Figures 5 and
21. ted warranty is sole and exclusive No other warranty is made whether expressed or implied including any warranty of merchantability or fitness for a particular purpose 2013 Expedeon Inc All Rights Reserved C C D d GELFREE 8100 Fractionation System 27 of 30 EX et Jeon Bibliography Tran J C Doucette A A 2008 Gel Eluted Liquid Fraction Entrapment Electrophoresis An Electrophoretic Method for Broad Molecular Weight Range Proteome Separation Analytical Chemistry 80 1568 1573 2013 Expedeon Inc All Rights Reserved C exPedeonearres 8100 Fractionation System 28 of 30 Cc protein discovery amp analysis Limited Use License Products GELFREE 8100 Fractionation System GELFREE 8100 Cartridge Kit The above Products and their use are protected by Expedeon Inc A person enterprise or institution which acquires the Product s from Expedeon or an authorized distributor or reseller of Expedeon products in each case a Buyer has a limited no transferable license to use the Product s for research purposes only and excluding any Non Licensed Use Buyer acknowledges that Expedeonis not obliged to transfer any additional know how or to provide consulting services to implement or use the Expedeon Technology The Buyer may transfer the Product s and or information or materials made through the Buyers use of the Product s to a scientific collaborator or otherwise if this is genuinely require
22. ts buffer solutions or cartridges that are not supplied by Protein Discovery Inc may cause damage to the unit and cause the warranty to become null and void Internet Visit the Expedeon website www expedeon com where you can Download information on our innovative new sample preparation products and special product offerings View and download the newest application notes technical notes and publications Download manuals in Adobe Acrobat PDF format Download GELFREE 8100 Cartridge Kit Quick Reference Cards in Adobe Acrobat PDF format Purchase GELFREE 8100 Cartridge Kits and reagents Request catalog and product literature and pricing Contact Us For more information or technical assistance Corporate Headquarters Expedeon Ltd Unit 1A Button End Harston Cambridgeshire CB22 7GX UK Phone 44 0 1223873364 Fax 44 0 1223 873371 Email info expedeon com 2013 Expedeon Inc All Rights Reserved expedeon GELFREE 8100 Fractionation System 26 of 30 protein discovery amp analysis User Notifications Limitations on Use This product is covered under pending U S and foreign patents Any reproduction in whole or in part without express written authorization is strictly prohibited Limited Warranty Expedeon is committed to providing our customers with the highest quality goods and services Our goal is to ensure that every customer is 100 satisfied with our products If
23. y Protein Discovery Note The GELFREE 8100 Fractionation Station is capable of supplying up to 300 V per channel However the voltage recommended for best results is specific to each cartridge type as more fully described on the Cartridge Kit Reference Card Important Notice The GELFREE 8100 Fractionation System is manufactured in the USA from domestic and international components To ensure safe reliable operation always operate the GELFREE 8100 Fractionation System according to the instructions provided in this manual Wear personal protective equipment e g gloves and safety glasses when working in a laboratory environment Expedeon products are intended for in vitro use only Expedeon is not responsible for injuries or damages caused by improper use 2013 Expedeon Inc All Rights Reserved E expede on GELFREE 8100 Fractionation System 6 of 30 protein JEO amp analysis Unpacking and Placement Unpacking and Inspection of Parts Upon receipt of your GELFREE 8100 Fractionation Station open the box carefully remove the foam packaging and remove the instrument and power cord from the box Remove the tape securing the lid along with the protective plastic so that the lid can operate freely The instrument could have been damaged during shipment Inspect the instrument case lid and hinges and electrodes for any obvious signs of damage If damage has occurred please notify Customer Service immediately
24. you should have any questions or concerns whatsoever about a Expedeon product please contact one of our Customer Service Representatives Expedeon warrants that all of its products will perform according to the specifications stated in the User Manual Expedeon warrants the GELFREE 8100 Fractionation System to be free of defects in parts and workmanship for one year from the date of shipment The company will replace free of charge any product that does not meet those specifications This warranty does not apply to defects resulting from actions of the user such as misuse improper wiring operation outside of specification improper maintenance or repair or unauthorized modification No warranty is granted for products beyond their listed expiration date No warranty is applicable unless all product components are stored in accordance with instructions This warranty limits Expedeon s liability only to the cost of the product Expedeon makes every effort to ensure the accuracy of its publications but realizes that the occasional typographical or other error is inevitable Therefore Expedeon makes no warranty of any kind regarding the contents of any publications or documentation If you discover any error in any of our publications please report it to one of our Customer Service Representatives Expedeon has no responsibility or liability for any special incidental indirect or consequential loss or damage whatsoever The above limi
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