Model PLI-90 Pico-Injector User`s Manual
Contents
1. The ratio of the two is the same at the tip as for the original capillary glass Choosing a pipette size and shape for intracellular injection is difficult Larger tips deliver more material but increase the risk of cell damage caused by leakage around the pipette while in the cell or later by incomplete sealing The smaller the cell the smaller the pipette tip it will tolerate The smaller the tip the more likely it is to clog Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Preliminaries For reference intracellular electrophysiologists routinely record for an hour or so from cells of 10 micron diameter with pipette tips of 0 1 micron inside diameter Larger tips can therefore be used for brief injection in such cells For nuclear injections a small er taper angle is needed to avoid leakage further up the shank of the pipette at the plasma membrane Although even intracellular injection can be done from below with an upright micro scope most injections are done from the side or from above the cells Four differ ent strategies have been used to suitably fix the cell in position for successful intracel Iular injection 1 For suspended cells a second larger pipette is used to hold the cell This pipette s tip is first polished with a microforge done by placing the pipette with in 5 microns of a hot filament for a few seconds With its axis horizontal it is moved to hold the cell with applied suct2. are set by the INJECT TIME switch 9 Pout connector The injection pipette is attached to this connector using the supplied output hose PLI OH Without the hose attached the port is closed In use the injection bal ance fill and clear pressures are delivered through this output connector 10 Ppalance control This seven turn control sets the balance pressure over the range from 0 1 to 10 psi about 7 70 kP Clockwise rotation increases the pressure and can be used to control the capillary action in the pipette Harvard Apparatus Model PLI 90 User s Manual Rear Panel Connectors and Switches Figure 2 Rear Panel AUDIO INDICATOR ON INPUT FILTER 70 105Pst_ O 5 FOOT SWITCH INPUTS L3 INJECT GATE Refer to Figure 2 above for the location of controls and connectors Numbers below refer to the order of listing below 1 FOOT SWITCH INPUTS connec tors An optional footswitch PLI FS can be connected to any one of these connec tors When connected to INJECT pushing the footswitch starts injection When con nected to GATE injection pressure con tinues as long as the footswitch is depressed 2 PRESSURE INPUT connector This connector is the input for the com pressed gas See Preliminaries Section for recommendations on the gas A maxi mum of 105 psi can be safely applied 105 psi is optimal At lower pressure gas Publication 5403 005 REV C will be used at a slower rate Avoid inpu
3. 40 3 59 30 1 1 5385 60 5 39 40 1 1 7180 80 7 18 50 1 1 8976 00 8 98 60 1 1 10771 20 10 77 1 1 5 22 44 0 02 10 1 5 224 40 0 22 20 1 5 448 80 0 45 30 1 5 673 20 0 67 40 1 5 897 60 0 90 50 1 9 1122 00 112 60 1 5 1346 40 1 35 1 1 10 179 52 0 18 10 1 10 1795 20 1 80 20 1 10 3590 20 3 59 30 1 10 5385 60 5 39 40 1 10 7180 80 7 18 50 1 10 8976 00 8 98 60 1 10 10771 20 10 77 1 1 75 75 74 0 08 10 1 75 757 35 0 76 20 1 75 1514 70 151 30 1 75 2272 05 2 27 40 1 75 3029 40 3 03 50 1 75 3786 75 3 79 60 1 75 454410 4 54 Constant includes unit conversion factors and effects of water like viscosity and needle taper for a cone balf angle of 10 medium taper Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Power Entry Module PEM Active Fuse P AC Line Contact 100 120 220 240 Spare Fuse Power Entry Module PEM Spare Fuse Y a A 48 Tabs For Fuse Drawer 2 Tabs For Fuse Assembly 2 Fuse Drawer Figure 5 Power Entry Module PEM and Fuse Drawer 1 With a small flat screwdriver squeeze tabs of fuse drawer and pull out 2 With a small flat screwdriver open tabs of fuse assembly very gentle to avoid breaking the tabs 3 Rotate fuse assembly to AC Line in use Re
4. Model PLI 90 Pico Injector User s Manual Model PLI 90 Pico Injector Users Manual MA1 65 0004 HARVARD APPARATUS Publication 5403 005 REV C WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and
5. aratus Model PLI 90 User s Manual Introduction The PLI 90 Pico Injector allows small liquid volumes to be delivered precisely through micropipettes by applying a regulated pressure for a digitally set period of timel2 The pressure is applied pneumatically compressed gas to deliver volumes from microliters to femtoliters from the same instrument This digital injection pressure is enhanced by two auxiliary pressures Balance A lower balance pressure applied to the delivery pipette between injections pre vents clogging caused by pipette movement as well as dilution of the injected material by capillary action Clear Momentary application of high pressure can be used to clear clogged pipettes Triggering of injection is accomplished two ways panel push button or optional foot switch The duration of injection can be determined by an internal clock set with a dig ital switch or the depression of an optional foot switch This manual assumes the reader has no previous experience with microinjection either extracellular or intracellular Those planning on larger volume delivery 100 pL and up can ignore the BALANCE feature Some reference will be made to a less pre cise technique for smaller volume delivery to highlight the precision determining fea tures of this instrument This technique involves pressurizing the gas in a macro syringe attached to a delivery micropipette by advancing the piston of the syringe with a micrometer
6. ard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This apparatus is not registered with the FDA and is not for clinical use on human patients A CAUTION Not for clinical use on human patients Publication 5403 005 REV C s Harvard Apparatus Model PLI 90 User s Manual General Safety Summary Please read the following safety precautions to ensure proper use of your pico injector To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury USE PROPER POWER SUPPLY The product is supplied with an approved power supply and line cord USE PROPER LINE CORD Use only the line cord shipped with the product and make sure line cord is certified for country of use GROUND PRODUCT This product is grounded through the grounding conductor of the power cord To avoid electric shock use only approved line cord with the product and insure it is connected to earth ground MAKE PROPER CONNECTIONS Make sure all conections are made properly and securely ORIENT EQUIPMENT PROPERLY Do not position the equipment such that it is difficult to reach the disconne
7. croinjection also involves other skills several other instruments and accessories and various supplies The purpose of this section is to give an overview of these tech niques for those new to microinjection some guidance on equipment selection is also supplied Required auxiliary equipment for microinjection includes a pipette puller and micro manipulator s Ideally the puller should be capable of making pipettes with tip diam eters in the 0 2 to 1 57 micron range with a short enough taper length for both mechanical strength and low flow resistance If most injections are to be extracellu lar then a puller suitable for extracellular patch clamp pipettes is satisfactory For intracellular injections some magnetic pullers may be suitable Alternatively a two stage gravity puller with variable weights can be satisfactory over the entire range The required three dimensional movement can be produced by an inexpensive mechani cally linked micromanipulator for large cells such as frog oocytes More commonly a hydraulic one for fine vibration free movement is mounted on a coarse mechanical manipulator Suitable equipment is available from Harvard Apparatus Inc Required supplies include compressed gas and microcapillaries Compressed air is suit able for oxygen insensitive injection material Nitrogen is a satisfactory inert gas for the general case A pressure of 105 psi is sufficient a regulator will be needed if sup plied from a bottle
8. cting device OBSERVE ALL TERMINAL RATINGS Review the operating manual to learn the ratings on all connections AVOID EXPOSED CIRCUITRY Do not touch any electric circuitry inside the product DO NOT OPERATE WITH SUSPECTED FAILURES If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection OBSERVE ALL WARNING LABELS ON PRODUCT Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal CAUTION CAUTION This product is not registered with the FDA and is not for FOR RESEARCH USE ONLY clinical use on human or veterinary patients It is intended NOT FOR CLINICAL for research use only USE ON PATIENTS Publication 5403 005 REV C E Harvard Apparatus Model PLI 90 User s Manual Gas Usage Warning MN WARNING Pico lnjector uses gas even when off To provide finely controllable output pressure the gas regulators are of the bleeding type Such regulators use gas even in the absence of ejections The Pico Injector thus uses gas even when off To eliminate this consumption and as a good safety practice turn off the gas supply at the source when the Pico Injector is not in use Plan accordingly Notice that the BALANCE is on continuously once an output hose is attached to the front panel without a micropipette attached even this gas usage can be significant Publication 5403 005 REV C E Harvard App
9. en holder s as fol z WA No lows SLO PLI PH1 amp PLI PH1A Unscrew the end of the holder with a 2 mm diame ter hole This end hex piece should be placed over the tube end Thread the hex Bison piece on the holder and dame Bushing Metal Sleeve Silicon Rubber Gasket 7 5mm L x 1 5mm ID Fig 4 PL PHI PHIA 1 5 mm OD Glass Pipette Assembly Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Using the Pico lnjector tighten firmly The 1 mm OD capillary is inserted in the opposite end of the holder through its o ring and its fitting is then tightened PLI PPH Unscrew the end of the holder with the 2 mm diameter hole and place it over the output hose PLI OH Reattach this fitting securely with the hose inserted as far as it will go The 1 5 mm OD capillary is similarly attached to the other end of the hold er through its O ring Attach the output hose to the front panel Tighten them secure ly so that the valve within this port is open allowing pressure to be controlled in the hose and connecting micropipette Balance The inflow into the pipette caused by capillary forces prior to or in between injec tions should be a small percentage of that being injected As the volume desired gets smaller the relative inflow gets larger even faster due to the smaller tip size being used To avoid this problem set the balance pressure before placing the pipette in the cell s external medium T
10. en percent of the injection pressure is a good starting value Exact balance is difficult to determine often the fastest way to handle this is to set the bal ance high enough that slight outflow is observed Balance is assessed by watching the movement of the liquid meniscus in the pipette The outflow left is still small com pared to the continuous streaming used in the continuous ejection technique If a filling fiber is used the capillary inflow is larger so a higher balance pressure is needed In general however when a balance pressure is being used such a fiber should not be used sometimes the injection material will run out spontaneously or the pressure needed to balance will abruptly change with time These may be due to par ticles lodging on the fiber If desired a rough estimate of the volume coming in can be made Focus the microscope on the liquid meniscus in the loaded pipette while its tip is still in air with the balance pressure set to zero Use an eyepiece reticle to meas ure the meniscus movement when liquid is then raised to cover the tip The inflow vol ume is approximately the difference between the volume of two cones The estimate is more precise for smaller initial volumes in the pipette Clear To clear a clogged micropipette push the CLEAR button momentarily once the tip has been removed from a cell Watch the tip region while this is done to see the brief movement of liquid denoting that the tip is clear Repeat i
11. er angle if the pulling force and or heat change during pulling Two stage pullers give two angles for example Delivery rate depends on the angle nearest the tip Stephens D L et al Easy to use equipment for the accurate microinjection of nanoliter volumes into oocytes Anal Biochem 114 299 309 1981 Brinster R L et al Proc Natl Acad Sci 82 4438 4442 1985 DNA into mouse oocytes Webster D R et al J Cell Biol 105 265 276 1987 tubulin into human fibrob lasts and hamster ovary cells Palmer M R et al in Electrophysiological Techniques in Pharmacology pages 169 187 1986 Alan R Liss Inc Various materials onto mammalian neurons mRNA into Xenopus oocytes see footnote 9 Stacey D W et al Exp Cell Res 171 232 242 1987 ras protein into tumor cells Pasti G et al Nature 324 375 377 1986 protein kinase C into Swiss 3T3 cells Silver R B Proc Nat Acad Sci 83 4302 4306 1986 antibodies into sand dollar embryo de Laat A M and Blaas J PI Sci 50 161 169 1987 cytoplasmic organelles into plant protoplasts Publication 5403 005 REV C 3 Harvard Apparatus Model PLI 90 User s Manual Tips Hose Connections The input and output hoses should be attached to their respective connectors If each connector s needle valve located in the micro injector body is not fully opened the airflow will be restricted or blocked To prevent this from happening check each con nector for t
12. f needed The clearing pres sure pulse has a preset duration of 500 msec to avoid excessive release if the clog clears immediately Repeat as needed Footswitches Optional footswitches can be used for inject These switches are plugged into the rear panel each is wired in parallel with the corresponding front panel switch See the instructions for each panel switch to see how they operate When a footswitch is used with the GATE connector the duration of injection is manually determined by the duration of pressing the footswitch Injection Volume Adjustment Adjust injection volume by changing either injection pressure injection duration or the micropipette inside tip diameter or taper The dependence on net injection pressure duration and pipette taper angle is linear the dependence on tip diameter is cubic Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Volume Calibration Chart Formula Volume in Nanoliters 17952 x tip I D in micron x Pressure in psi x Time in sec Example Volume 17952 x 5 X 10 X 1 Nanoliters 224 40 Nanoliters Pressure Time Pipette Tip p s i sec _1 D um Femtoliters Picoliter Nanoliter Microliter Milliliter 1 1 0 1 179 52 0 18 10 1 0 1 1795 20 1 80 z 20 1 0 1 3590 40 3 59 30 1 0 1 5385 60 5 39 z 40 1 0 1 7180 80 7 18 50 1 0 1 8976 00 8 98 60 1 0 1 10771 20 10 77 1 1 1 179 52 0 18 10 1 1 1795 20 1 80 20 1 1 3590
13. ightness by turning clockwise This will ensure needle valve depression Setting Pressures and Time Pulses A Balance Pressure Set the balance pressure while viewing the pipette under magnification This method will help the user to stabilize the solution within the pipette easily B Injection Pressure and Time Pulse Setting the initial injection pressure low prevents the loss of solution To easily obtain the desired pressure setting set the time pulse on 1 one second with the injection pressure set at its minimum Trigger the time pulse while viewing under magnification Increase the injection pressure until the solution within the pipette begins to flow out the tip opening The pressure shown on the LCD can now be used as the initial injection pressure setting Adjust the injection pressure and tim ing to obtain the desired injection Publication 5403 005 REV C
14. ion The injection pipette is also straight and is inserted horizontally from the opposite side This geometry avoids damage to the cell membrane caused by shearing forces The optics are straightforward because the pipettes remain in focus as they are advanced 2 Forcells that can be or are attached to a surface in a closely packed layer straight injection pipettes can also be used In this case the pipette axis slopes slightly down from the horizontal The tendency of the cells to slide when the pipette enters is resisted by the extracellular environment or attachment to the culture surface The microscope should be focused on the cell s surface The pipette tip then comes into focus just before injection If the cell is nearly spherical the hardest case the pipette should again enter the cell membrane at right angles to avoid shearing Non spherical cells for example cultured fibroblasts have a more robust cytoskeletal structure so the pipette can be pushed in even if not perpendicular to the membrane surface 3 Forless firmly attached cells the injection pipette can be bent near the tip after pulling The pipette s main axis slopes slightly down from the horizontal The angle of bend should allow the tip to point straight down With an inverted microscope the tip is viewed through the cell as it is lowered for injection The microscope is focused on the cell s top surface and the tip comes into focus just before insertion Again sheari
15. kmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc S October Hill Road Building 7 Holliston Massacbusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harv
16. ng forces are avoided Suitable bends can be made with a microforge a simple way to do this is to move the pipette near a hot filament at the position of desired bend The tip will spontaneously bend away 4 In all of the above techniques a three dimensional micromanipulator controls the movement of the injection pipette If this straight pipette is instead attached to a condenser mount inverted scope then a one dimensional manipulator can be used The remaining two directions of manipulation are done with stage micrometers moving the vertical injection pipette over each cell in turn If the vibrations transmitted with the condenser mounting are manageable then this approach gives the fastest rate of cell injection Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Front Panel Controls and Connectors Figure 1 Front Panel PRESSURE PSI Lo h INJECT INJECT CLEAR TIME P out 1 Greenvale NY 11548 PLL90 P balance inject G X Pelear GAUGE PRESSURE SOURCE Refer to Figure 1 above for the location of controls and connectors Numbers below refer to the order of listing below 1 INJECT pushbutton Push this to manually trigger the injection pressure for a time set by the internal timer The switch remains lit for the dura tion of the injection 2 INJECT TIME pushbutton This determines the time multiplier for the internal injection timer 10 msec and 1 0 sec mul
17. of compressed gas Optional equipment for microinjection includes a microforge to bend a micropipette or to polish a pipette tip for holding a cell a microgrinder to bevel the pipette tip to increase the delivery rate without additional cell damage and an micro incubator to hold the cells at incubation temperatures during microinjection Suitable equipment is available from Harvard Apparatus Inc Accessories are also available to enhance your Pico Injector see Price List Micropipettes are made with a pipette puller from a microcapillary 1 2 mm in diam eter by heating some 3 10 mm of its length with a concentric heater while applying a force gravitational or magnetic to pull both ends of the capillary apart Two micropipettes are produced per capillary Two useful distinguishing parameters of the micropipette are the inside diameter of its tip and the angle of taper to the tip The smaller this angle the longer the tapered region The larger the tip the more material is delivered for the same applied pressure and time Just a 10 decrease in diameter decreases the delivery rate by over 30 A 10 decrease in taper angle longer taper would decrease the delivery rate about 10 The extreme sensitivity of delivery rate on tip diameter makes it important to have a reproducible pipette puller If you use published tip sizes as a starting point distinguish between the relevant inside diameter and the more visible outside diame ter
18. place fuse according to fuse rating 100 120 5 Amp Fuse 220 240 5 Amp Fuse 5 Assemble fuse drawer into Power Entry Module PEM until you hear a click AC Line Contact lt AC Line Contact i owt E Y o o e eo kej uL rna E o oO is a a N Assembly for 100 120 Assembly for 220 240 Window to read AC Line used Figure 6 Bottom View Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Footnotes and References 10 11 12 13 14 15 16 17 McCaman R E et al A pressure system for intracellular and extracellular ejec tions of microliter volumes Brain Research 142 141 1977 Palmer M R et al Physical and physiological characteristics of micropressure ejection from pipettes Neuropharmacology y 19 931 938 1980 Hiramoto Y Exp Cell Res 27 416 426 1962 Pneumatic micrometer syringe mercury See Kiehart D P Methods Cell Biol 25 13ff 1982 for a careful expla nation Graessmann A Exp Cell Res 60 373 382 1970 Mario Capecchi private communication Dennis Stacey private communication 10 micron pipettes would be suitable for frog oocytes but there is little need for the sizes between 1 5 and 10 microns Pipettes will have more than one tap
19. screw Ejection takes place continuously from the pipette the vol ume delivered depends both on the unregulated over pressure in the syringe and the poorly known time the pipette is left inside the cell This continuous ejection tech nique supposedly reduces the frequency of pipette clogging Reliable microinjection also requires skill in making and using micropipettes Authorities on microinjection state that the most important single factor in microin jection is the micropipette The Pico Injector with its reproducibility simplifies the search for the optimum micropipette Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Specifictions Specifications Input Gas Pressure Injection Pressure Balance Pressure Clearing Pressure Injection Time Pressure Display Duration Mode Trigger Mode Power Foot Switch es Accessories Supplied Weight Dimensions 70 to 105 480 to 720 kPa 0 2 to 60 0 psi 413 kPa Regulated Multi Turn Control 0 1 to 9 9 psi 68 9 kPa Regulated Multi Turn Control Inlet Pressure Unregulated 01 to 99 sec 1 to 99 sec Digital 3 5 digits Internally timed or externally gated Foot Panel Switch 115 230 VAC 50 to 60 Hz 35 Watts Inject and Gating Optional Input Hose Output Hose and Power Cord 15 Ibs 6 8 kg 15 in x 10 in x 5 in 38 cm x 25 5 cm x 11 cm Publication 5403 005 REV C E Harvard Apparatus Model PLI 90 User s Manual Preliminaries Mi
20. t pressures less than 105 psi 3 INPUT FILTER The PICO INJECTOR is supplied with an input filter It traps particles larger than 0 1 micron as well as the liquid often present in a building s compressed air lines Drain by pushing the button on the underside of its transparent case 4 AUDIO INDICATOR switch When ON a buzzer sounds for the dura tion of injection Turn it OFF if this audi ble monitor is not desired Harvard Apparatus Model PLI 90 User s Manual Using the Pico Injector General Considerations It is much easier to reliably inject large volumes than small ones For large volumes the balance pressure capability is not needed Pipettes seldom clog so the clear capa bility is also not needed The dividing line between large and small is not rigid it depends on how quantitative a delivery is required That volume line would typically be in the 10 100 pLiter range For convenient visualization and approximate geomet ric measurement 1 fLiter is a cube 1 micron on a side or a sphere 1 24 microns in diameter 1 pLiter is a cube 10 microns on a side or a sphere 12 4 microns in diame ter while 1 nLiter is a cube 100 microns on a side or a sphere 124 microns in diame ter Because volume goes as the cube of linear dimensions such geometric volumes are imprecise but often useful Extracellular delivery is nearly always large Intracellular is often of small volumes but not for frog oocytes A more quantitati
21. tipliers are available 3 CLEAR pushbutton Push this briefly to deliver a half second pressure surge to clear a clogged pipette The pressure applied is the supply pres sure If the button is left pushed in for longer than a half second the clearing surge is extended The button remains lit for the duration of the clear 4 PRESSURE display This three digit display gives the gauge pressure selected by the PRESSURE METER SOURCE switch 5 PRESSURE METER SOURCE switch This switch selects various places inside the PICO INJECTOR for reading the pres It does not change the pressure applied to the output Pinject is the pres sure applied to the output during injec tion Pratance iS the pressure applied to the pipette when injection is not taking place Pelear is the pressure externally sup sure Publication 5403 005 REV C plied to the input on the rear panel and used in the CLEAR mode Pout is the pres sure currently applied to the output port 6 Pinject control This seven turn control is used to set the injection pressure over the range from about 0 4 to 60 0 psi about 2 8 413 kP Clockwise rotation increases the pressure 7 POWER pushbutton Push this button once to apply AC power to the unit The button will light Push it again to turn the instrument off 8 INJECT TIME digiswitch This two digit switch is used to set the duration of injection when timed internal ly The units 10 msec or 1 sec
22. ve way to dis tinguish between large and small is given in the balance section below Interconnections and Initial Setup Connect the gas input hose PLI IH to the rear panel input connector of the Pico Injector Connect the other end with optional input hose adapter if needed to the gas supply Turn on the POWER switch and verify the input gas pressure with the dig ital meter by setting the PRESSURE METER SOURCE switch to Pelear Practice with the inject and balance pressure controls by first turning the METER SELECT switch and then adjusting each in turn With non zero values of inject and balance pressure set the PRESSURE METER SOURCE switch to Pout NOTE Tbe PRESSURE METER SELECT switcb must be set to Hex Nut Pout to read cbanges in pres sure on tbe display or monitor output Set tbe inject time to Holder five seconds and push tbe panel INJECT switcb to see tbe tempo rary cbange in pressure from balance to inject and back A buzzer will sound during injec tion If tbis is not wanted turn off tbe switcb on tbe rear panel Output Hose Silicon Rubber Gasket bmm L x 1mm ID One output hose PLI OH US MS sien is supplied with the Pico Injector These are designed for any of the three optional Fig 3 PLEPHI PHIA 1 0 mm OD Glass Pipette Assembly pipette holders PLI PH1 Knurled Nut Glass Tube PLI PH1A PLI PPH described in the price list Hex Nut Connect the PLI OH hose to Output Hoss the chos
23. will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 5 Harvard Apparatus Model PLI 90 User s Manual Table of Contents SUBJECT PAGE NO General Informantion Warranty 2 General Safety Summary 3 Gas Usage Warning 4 Introduction mana E 5 Specifi atiONS iii a ek ela dei 6 Preliminaries riore cec ine di eni 7 Front Panel Controls and Connectors 9 Rear Panel Connectors and Switches 10 Using the Pico Injector 11 Volume Calibration Chart 13 Power Entry Module PEM 14 Footnotes and References 15 MDS ii ndo m ke Ne 16 Publication 5403 005 REV C Harvard Apparatus Model PLI 90 User s Manual Warranty and Repair Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to wor
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