Milton Roy Spectronic SP501 601
Contents
1. SECTION 5 OPERATION 5 1 BASIC SPECTROPHOTOMETRIC MEASUREMENTS To use the SPECTRONIC 501 601 for basic spectrophoto metric measurements absorbance transmittance or con centration a Turn Power on b Await completion of SELF TEST c Enter analytical wavelength d Press lt SECOND FUNCTION GO TO gt e Allow 30 minute lamp warm up f Press lt T A C gt to choose desired data mode g Open sample compartment door insert blank into sample holder h Close sample compartment door i Press lt SECOND FUNCTION gt lt 100 T ZERO A gt j Open sample compartment door and remove the blank k Insert sample into sample holder and close sample com partment door 1 Read data that is displayed or press lt SEND gt if a printer or computer is installed 5 2 TEST PARAMETER STORAGE The SPECTRONIC 501 601 can store one set of test parame ters as the default values Once stored these values will be present the next time you turn your spectrophotometer on The parameters that will be stored are current wavelength e lamp status including lt LMP SAV gt status lamp interchange wavelength data mode lt T A or C gt factor signal averaging value high low limits RS 232 interface parameters baud rate no of stop bits and parity etc To save these parameter values using Diagnostic 4 e Enter the desired wavelength and press lt GO TO A gt Set all t2. LIST CATALOG gt SELECT are active only when the Accessory Control Module is installed and turned on iii SECOND FUNCTION SECOND FUNCTION 10 LIM LMP SAV LMP CH SECOND FUNCTION SECOND FUNCTION SIG AV SECOND FUNCTION DEUT SECOND FUNCTION SECOND SECOND FUNCTION SECOND FUNCTION FACTOR DIAG TUNG Display or set lower limit If data falls below this limit out of range flag an asterisk will appear on the display Displays or sets tungsten lamp save on off Display or set lamp interchange wave length SPECTRONIC 601 only Display or set upper limit If data ex ceeds limit an out of range flag an as terisk will appear on the display Display or set signal averaging function Turn Deuterium Lamp on or off 601 only Display or set concentration factor Access diagnostic tests Turn Tungsten Halogen Lamp on or off 601 only This manual presents basic information on the use of the SPECTRONIC 501 601 spectrophotometer Terms contained with lt gt are names of keys found in the keyboard or appropriate alphanumeric key entries LIMITED WARRANTY SPECTRONIC instrumentation and related accessories are warrantied against defects in materials and workmanship for a period of 1 year from the date of delivery This warranty is provided only if the warranty registration card is returned to Milton Roy within fi
3. 601 100 T ZERO EDIT FACTOR DIAG TUNG G0TO X ust SIG AV DEUT C amp C COGOC CATALOG LMPSAV LMPCH KEYBOARD ii SAMPLE COMPARTMENT SECOND FUNCTION SELECT CLEAR 100 T ZERO A SECOND FUNCTION GOTOX 4 50 T0 X SECOND FUNCTION t ust EDIT CATALOG TUNG DEO EDIT SECOND LIST SECOND FUNCTION CATALOG SECOND FUNCTION SELECT SECOND FUNCTION SUMMARY OF KEYBOARD FUNCTIONS Activate a key s second function Send data to optional RS 232 interface for printer Accessory Control Module or remote computer Delete erroneous data entry Set Transmittance Absorbance or Con centration data mode Set transmittance data to 100 or ABS data to 0 0A Move monochromator up in wave length Move monochromator down in wave length NOTE The lt YES gt and lt NO gt keys function as accept or reject keys only when the spectrophotometer is used in con junction with an APPLIPAK cartridge and the Accessory Control Module or certain diagnostics Cause monochromator to go to entered wavelength Numeric entry keys Edit current test parameters List current test parameters on display or printer Catalog list of stored tests on display or printer Choose a stored test from those included in catalog NOTE SECOND FUNCTION EDIT
4. 601 only Ensure that the wavelength is set to a value greater than 400 nm and press lt SECOND FUNCTION gt lt DEUT gt to turn off the deuterium lamp The lamp indi cator on the display should be a T Remove the Maintenance Lamp Access Door by loosening the Lamp Maintenance Compartment Access Screw three to five complete turns and lifting up the door at the back edge Figure 6 3a Correct 14 601 only Use a flatblade screwdriver with an insulated handle to turn the Deuterium Lamp Adjustment Screw 21 until the instrument displays a minimum absorbance reading If the instrument displays a negative absorbance reading stop and repeat step n p 601 only Repeat step o q When adjustments are complete turn the instrument off and then on again to re initialize it r Reposition the Maintenance Lamp Access Door and se cure it by tightening the Lamp Maintenance Compartment Access Screw c Figure 6 3b Incorrect Figure 6 3 Light Hitting and Passing Through the Filter 335103 100018 SECTION 7 RS 232 C INTERFACE INTRODUCTION The RS 232 C Interface provides a 2 way communications link between the SPECTRONIC 501 601 spectrophotometer and an external electronic device such as the Accessory Control Module a computer or a printer The RS 232 C Inter face consists of the following components a A printed circuit board which is installed inside the spec tropho
5. wavelength n n 195 to 999 for SPECTRONIC 601 n 325 to 999 for SPECTRONIC 501 Turns the DEUTERIUM LAMP ON n 1 or OFF n 0 601 only Turns the TUNGSTEN LAMP ON n 1 or OFF n 0 601 only Outputs the current LAMP CHANGE wavelength 601 only Sets the LAMP CHANGE wavelength to n nanometers 320 to 380 nm 601 only Turns the LAMP SAVE mode ON n 1 or OFF n 0 Outputs the current HARDWARE ANALOG GAIN value Sets the HARDWARE ANALOG GAIN to n The data field must consist of two characters in hexadecimal format range n 00 0F This value is used by the 501 601 to establish zero A 100 T levels The HDG and ZRR commands permit multiple wavelength testing see Appendix B for examples 335103 10001B FLF Empties the data filter Data characters are ignored ECH n Turns the CHARACTER ECHO mode ON n 1 or OFF n 0 CCM n Turns the COMMAND COMPLETION ANSWERBACK mode ON n 1 or OFF n 0 FLRn Turns the FLASHING R function ON n 1 or OFF n 0 When set to ON an R character will flash on the display FLT n Turns the FLASHING T function ON n 1 or OFF n 0 When set to ON a flashing T character will appear on the display RST Executes a POWER ON RESET DIAn Executes a diagnostic test number n refer to Section 6 4 of the SPECTRONIC 501 601 Operator s manual NOTE Not all diagnostics are designed to work with the serial ports ZRR Outputs the data
6. CA BALTIMORE MD RALEIGH NC SAN FRANCISCO CA BOSTON MA COLUMBUS OH ORLANDO FL NEWARK NJ HOUSTON TX ATLANTA GA ROCHESTER NY SEATTLE W A CHICAGO IL CHARLOTTE NC TORONTO ONT CANADA To obtain service for your Milton Roy instrument contact our Service Operations Center at the following TELEPHONE Inside New York State 800 327 7053 Outside New York State 800 654 9955 Alaska Hawaii and Canada 716 248 4235 U TELEX 444 1037 FAX 716 248 4014 To purchase parts supplies or accessories for vour Milton Rov instrument contact our Customer Service Department at 820 Linden Avenue Rochester NY 14625 TELEPHONE Inside New York State 716 248 4117 and Alaska Hawaii Canada 716 248 4044 Outside New York State 800 922 0826 TELEX 444 1037 FAX 716 248 4014 INTERNATIONAL MILTON ROY COMPANY MILTON ROY HONG KONG LTD MILTON ROY KK Analytical Products Division Room 808 810 Kornhill Metro Tower Takanawa Daiichi Building E Vlietinckstraat One Kornhill Road 2 21 41 Takanawa B 8400 Oostende BELGIUM Quarry Bay HONG KONG Minato Ku Tokyo 108 JAPAN Telephone 59 50 89 92 Telephone 5 8854613 Telephone 03 473 3181 TELEX 846 83092 Facsimile 5 674447 TELEX 781 242 4296 ANSB 83092 MILTON B TELEX 780 77401 ANSB MRCTYOJ ANSB MRHKL HX 335001 100 Rev J P8A19836 Printed in USA
7. MILTON ROY SPEGTRONIG t DUI SPEGTROPHOTOMETERS lt S ey PERATOR S INSTRUCTIONS MILTON ROY DPEGTRONIG D SPEGTROPHOTOMETERS OPERATOR S INSTRUCTIONS 900 WARM UP PRE OPERATIONAL SEQUENCE a Plug power cord into appropriate power source b Turn on spectrophotometer move back panel toggle switch to the right c The message SELF TEST will appear on the 501 601 s display while the instrument performs a pre operation self check d Approximately twenty seconds after you switch the power on SELF TEST will be replaced by the message GOING TO XXX NM XXX pre programmed wavelength value and then by wavelength and data values NOTE For effective operation the spectrophotometer must warm up for one half hour after being turned on Allow an additional 15 minutes for stabilization when you turn a lamp on or off Allow an additional 5 minutes for stabilization when switching the LAMP SAVE function for the tungsten lamp on or off NOTE Check the voltage label plate on the spectrophotometer s back panel to determine what voltage 100 115 220 or 240 has been set before you plug your SPECTRONIC 501 or 601 in If the voltage shown on the label plate is not the same as the voltage of your power source do not plug the instrument in call your authorized Be s 0mimisumia Sales Service representative to ar range for a voltage conversion BACK PANEL
8. No answerback 335103 10001B DEU n DIAn DMD ECH n FAC FAC FLF FLR n FLT n GTO n HDG HDG n HIL HILn KLK n LCH LCH n LOL LOL n LSVn RST SIG SIG n SND TRN TUN n ZER ZRR ZRR n 335103 10001B APPENDIX A ALPHABETICAL LIST OF COMMANDS Sets the ABSORBANCE mode Sets the ABSORBANCE mode and ABSORBANCE value n An audible device in the 501 601 beeps for about one half second Turns the COMMAND COMPLETION ANSWERBACK mode ON n 1 or OFF n 0 Causes 501 601 to chirp for about one twentieth of a second Sets the CONCENTRATION mode Sets the CONCENTRATION mode and CONCENTRATION value Executes a POWER ON RESET The 501 601 interprets this code as a BEP BEEP command Read Keyboard Immediate operation Executes a SND operation The 501 601 exits the DISPLAY DMD mode Similar to a reset and used primarily to purge buffers The 501 601 interprets this code as a CHP CHIRP command Turns the DEUTERIUM LAMP ON n 1 or OFF n 0 601 only Executes a diagnostic test Enters the DISPLAY mode Turns the CHARACTER ECHO mode ON n 1 or OFF n 0 Outputs the current FACTOR value Sets the FACTOR Empties the data filter Turns the FLASHING R function ON n 1 or OFF n 0 Turns the FLASHING T function ON n 1 or OFF n 0 Causes 501 601 to go to wavelength n Outputs the current HARDWARE ANALOG GAIN value Sets the HARDWARE ANA
9. quality and reliability never will The Milton Roy Company is proud to be the owners of both the internationally known SPECTRONIC product line and the excellent service and support network behind it Your SPECTRONIC instrument is fully supported by Milton Roy s Technical Support Group the same team of application specialists that has helped thousands of other SPECTRONIC owners achieve the best return on their invest ment The division s factory trained Field Service Engineers will continue to provide prompt emergency and maintenance services whenever they re required To ensure that we continue to meet your needs we need to hear from you Please take a few minutes to complete and return the enclosed warranty card In return we ll send you an attractive gift It s our way Of thanking you for your support and it will help you remember that our name is Milton Roy We re a strong growing company fully dedicated to continuing the SPECTRONIC product line s heritage Sincerely WEG led W Barry Gilbert President MILTON ROY IIB 9 HOW TO OBTAIN AUTHORIZED MILTON ROY SERVICE FOR YOUR INSTRUMENT If your instrument develops a malfunction that cannot be corrected by operator maintenance it can be serviced by trained specialists at a Milton Roy Service Center This service can be performed at your location or you may ship the instrument to our facility We currently have service locations in the following major cities LOS ANGELES
10. 335103 100018 6 4 MAINTENANCE amp TROUBLESHOOTING 6 4 1 Troubleshooting Chart If you experience the problems listed in the INDICATION INDICATION No display with unit turned on Display does not read 0 0 T with occluder block in light path Excessive drift and or noise Data display locked up Incorrect reading in data display 335103 100018 column check through the PROBABLE CAUSE and AC TION REQUIRED columns in sequence a through b and so forth PROBABLE CAUSE a Unit not plugged in b Outlet is dead c Fuse is blown d Part malfunction a Not in T mode b Part malfunction a Bubbles or particles in sample b Tungsten halogen lamp burned out c Deuterium lamp burned out d Sample compartment windows dirty e Lamps not aligned properly f Part malfunction a Computer or printer connected to optional serial port not on line or ready b Microprocessor error or part malfunction Incorrect blank Insufficient sample volume Bubbles or particles in sample Incorrect wavelength setting Wavelength error Electronic calibration error Part Malfunction mrar ACTION REQUIRED Plug in unit Change outlet Change fuse Call Milton Roy Service Press lt T A C gt until in T mode Call Milton Roy Service Check sample preparation Replace lamp Replace lamp Clean windows Refer to Section 6 5 Call
11. 335103 10001B 3 20 SECOND FUNCTION lt TUNG gt turns the tung 321 3 22 sten lamp on or off 601 only SECOND FUNCTION lt DEUT gt turns the deu terium lamp on or off for SPECTRONIC 601 only The current lamp status is indicated by the last charac ter on the right of the display T indicates that the tungsten lamp is on e D indicates that the deuterium lamp is on B indicates that the both lamps are on NOTE If you attempt to 1 turn the deuterium lamp off when the unit is below the lamp interchange wavelength or 2 turn the tungsten halogen lamp off when the unit is above the lamp interchange wavelength the message LAMP REQUIRED will be temporarily dis played and the normal data display will return This cannot be over ridden by continually pressing the key To turn both lamps off enter 1 SECOND FUNCTION lt DIAG gt The lamps will remain off and the display will read STANDBY until you press another key to return to the normal data display SECOND FUNCTION gt LMP CH gt displays or sets the lamp interchange wavelength see Section 4 6 Lamp Interchange Wavelength SPECTRONIC 601 only SECTION 4 DETAILED FEATURE DESCRIPTION 4 1 HIGH AND LOW LIMITS lt HILIM gt and lt LOLIM gt Use HI LIM and LO LIM gt to specify the range of test result values that you require Once you have specified the limits any final answer that falls outside the s
12. 9999 Outputs the current SIGNAL AVERAGE value Sets the SIGNAL AVERAGE to n n values may be only 0 fast 1 medium or 2 slow ACTION CAUSED BY THIS COMMAND Outputs current wavelength data mode and data value per selected format computer or printer An audible device in the 501 601 beeps for about one half second Causes 501 601 to chirp for about one twentieth of a second Turns the KEYBOARD LOCK mode ON n 1 or OFF n 0 When set to ON the 501 601 will not execute keyboard commands but each key closure will generate an ASCII character corresponding to the key pressed see Appendix C Enters the DISPLAY mode All uppercase letters all numbers and the following punc tuation marks can be sent to the display amp and An ASCII CR will blank the display between the last character sent and the end of the display and the next character will be displayed in position 1 An ASCII LF will blank the entire display and the next character will be displayed in position 1 Lowercase letters a through p will position the internal cursor so that the next dis playable character will be displayed in positions 1 16 respectively numbered left to right All control characters will be recognized during the DMD mode NOTE A single period does NOT increment the cursor position A lowercase z will exit the display mode Causes 501 601 to go to
13. G C and X are not echoed f FORMAT COMPUTER or PRINTER This specification controls the format of the data output from the 501 601 The com puter and printer formats will be fully explained in Section 9 g CONTROL S CONTROL ON or OFE When this function is ON the spectropho tometer will stop sending data when it receives a CON TROL S character It will not start sending data again until it receives a CONTROL character When this function is OFF the spectrophotometer will ignore both control characters h COMMAND COMPLETION ANSWERBACK ON or OFE When set to ON the COMMAND COMPLE TION ANSWERBACK function is activated If the spectrophotometer receives and executes a com mand properly it will transmit the ASCII characters OK followed by the programmed terminator back to the ex ternal device If the spectrophotometer receives an invalid command terminator or a valid command which cannot be exe cuted it will transmit the ASCII characters ER followed by the programmed terminator back to the external device e If the spectrophotometer receives a command that re quests data it will transmit the following sequence back to the external device the requested data the programmed terminator the ASCII characters OK and the pro grammed terminator again Step 2 When you complete this routine the RS 232 C Interface will be set up as you have specified It will remain this way
14. currently residing in the zero register in 8 character hexadecimal format ZRR n Sets the zero register to n The data field must consist of 8 characters in hexadecimal format In ZRR format ASCII characters for hexadecimal values 0 through F are entered in the following manner where IIII Whole integer value contained in the zero register FFFF Fraction integer values contained in the zero register ZERO REGISTER VALUE Illl FFFF 65536 This data is used by the spectrophotometer in establishing the zero A or 100 T level The ZRR and HDG commands permit multiple wavelength tests See Appendix B for examples 9 4 IMMEDIATE COMMANDS FROM THE COMPUTER TO THE SPECTROPHOTOMETER DECIMAL COMMAND EQUIVALENT RESULTING ACTIVITY CONTROL C 3 Executes a POWER ON RESET CONTROL R 18 Executes a SND operation CONTROL X 24 Similar to a reset and used primarily to purge buffers The serial input command interpreter is reset to an idle state This command does not terminate a command execution that is already in progress CONTROL T 20 The 501 601 exits the DISPLAY DMD mode CONTROL G 7 The 501 601 interprets this code as a BEP BEEP command CONTROL W 23 The 501 601 interprets this code as a CHP CHIRP command CONTROL K 11 Read Keyboard Immediate operation Displays an asterisk when no keys have been pressed otherwise displays ASCII character of the key pressed Refer to Appendix C
15. displayed value and proceed to the next firm ware function press lt YES gt on the spectrophotometer keyboard To refuse a displayed value and have another value dis played press lt NO gt Continue pressing lt NO gt until the required value is displayed When selecting the firmware functions and the various speci fications presented below be sure to check the user s guides and other documentation provided with the external device or the software programs used with the device a BAUD RATE 110 150 300 600 1200 2400 4800 or 9600 b PARITY ODD EVEN or OFF ODD or EVEN parity represents the checksum of the first 7 bits of each received or transmitted character When parity is OFF the eighth bit of each received character will be ignored when it is received and will be transmitted as a space If a parity error is detected on reception the input buffer will be emptied and the message RS 232 Error will appear on the spectrophotometer s display c NUMBER OF STOP BITS ONE or TWO d OUTPUT TERMINATOR CARRIAGE RETURN CR LINE FEED LF CARRIAGE RETURN immediately followed by a LINE FEED CRLF LINE FEED immediately followed by a CARRIAGE RE TURN e CHARACTER ECHO ON or OFE When set to ON the CHARACTER ECHO function is activated and the 501 601 transmits each char acter it receives back to the display of the external device 16 Exception Control characters S Q R T W
16. until you run the procedure again or until you turn the instru ment s power off Step 3 To save this setup in the instrument s non volatile memory execute Diagnostic 4 enter 14 lt SECOND FUNCTION gt lt DIAG gt The option will then be properly set up when ever power is turned on 335103 10001B SECTION 9 PROGRAMMING GUIDELINES 9 1 MESSAGES FROM THE SPECTROPHOTOMETER TO AN EXTERNAL DEVICE You may select either of two output formats computer or printer depending upon the device connected to the RS 232 C port This format has already been selected if you have performed Diagnostic 1 the Firmware Setup Procedure described in Section 8 9 1 1 Printer Format When you have specified printer format in the firmware setup procedure a string in the printer format shown below is sent out the RS 232 port when you press the lt SEND gt key on the 501 601 or in response to a remote SND command Example CWWWNMbSD DDDLMbXZ Explanation of Symbols C one of the following characters R indicates that a cartridge test is running T indicates that the temperature of the Thermoelec tric Flowcell has not yet reached the preset value The designation has priority over the designation SPACE neither an R or T is flashing in the display WWW wavelength NM ASCII characters NM representing nanometers b a space s sign is transmitted for a negativ
17. 0 PRINT D REM PRINT THE DATUM ON THE CRT 2 Obtain wavelength and absorbance data referenced to air of a sample from 900 to 800 nm at 2 nm intervals Correct the sample data for background absorbance FORMAT COMP ANS BACK ON 10 DIM Z 11 H 11 D 11 20 LET X 1 100 REM FIRST GOTO EACH WAVELENGTH SET ZERO ABS AND RECORD ZRR AND HDG 110 FOR WV 900 TO 800 STEP 10 120 PRINT 5 GTO WV REM GOTO THE DESIRED WAVELENGTH 130 INPUT 5 A REM WAIT FOR OK WHEN THE GOTO IS DONE 140 PRINT 5 ZER REM SET ZERO ABSORBANCE 150 INPUT 5 A REM WAIT FOR COMPLETION 160 PRINT 5 HDG REM ASK FOR THE HDG VALUE 170 INPUT 5 H X REM READ IN THE HDG VALUE 180 INPUT 5 A REM GET THE OK 190 PRINT 5 ZRR REM ASK FOR ZRR VALUE 200 INPUT 5 Z X REM READ IN THE ZRR VALUE 210 INPUT 5 A REM GET THE OK 220 LET X X 1 230 NEXT WV REM DO THE NEXT WAVELENGTH 300 REM AT THIS POINT THE PROGRAM SHOULD PAUSE AND PROMPT 310 REM THE OPERATOR TO LOAD THE SAMPLE COMPARTMENT WITH THE 320 REM MATERIAL TO BE MEASURED 400 REM NOW COLLECT THE DATA 410 LET X 1 420 FOR WV 900 TO 800 STEP 10 430 PRINT 5 HDG H X LOAD THE HDG VALUE 440 INPUT 5 A 450 PRINT 5 ZRR Z X LOAD THE ZRR VALUE 460 INPUT 5 A 470 PRINT 5 GTO WV REM GOTO THE WAVELENGTH 480 INPUT 5 A 490 REM AT THIS POINT A WAIT LOOP IS NEEDED FOR SETTLING OF 500 REM THE DATA R
18. 01 10001 SPECTRONIC 501 601 Operator s Manual 335101 10020 SPECTRONIC 501 601 Service Manual 332879 2 0 ampere Slo Blo fuse box of 5 335101 164 Dust Cover For a list of accessories that are available for use with the SPECTRONIC 501 601 spectrophotometers contact your authorized dealer or Milton Roy sales representative 6 3 DIAGNOSTICS To run a diagnostic program e Enter lt appropriate value gt The display will read ENTRY followed by the value e Press lt SECOND FUNCTION gt e Press lt DIAG gt USER DIAGNOSTICS DIAG FUNCTION 0 POWER ON RESET Executes a POWER ON reset in the software al lowing you to re initialize the instrument from the SPECTRONIC 501 601 keyboard Allow 5 minutes for lamp to stabilize after using lt DIAG gt 0 1 STANDBY Displays the message STANDBY turns both 335103 100018 USER DIAGNOSTICS DIAG FUNCTION lamps off and waits for any keystroke Any key stroke will turn the appropriate lamp s back on and return to normal data display Allow 15 minutes for lamp to stabilize REVISION LEVEL Displays the revision level of the firmware per manent stored programs in the instrument The display lasts one second For a longer display hold down any key while the display is present DISPLAY TEST Checks the spectrophotometer s display by test ing all segments First sixteen s will appear then sixteen 0 s The final display scrolls from left to right prese
19. Deuterium Lamp Connector Wires Be sure the red wire is connected to the center socket 13 g Seat the new lamp assembly in the instrument and replace d Examine the illuminated filter on the filter wheel to deter the thumbscrew mine the position of the light Light should be centered on h Tighten the Deuterium Lamp Assembly Screw 26 the filter and on the slit as shown in Figure 6 3a i Wipe the lamp with a clean lint free cloth to remove any e If light is not passing through the center of the filter as fingerprints shown in Figure 6 3b adjust the Tungsten Halogen Lamp j Perform the alignment procedure in Section 6 6 before 22 and or the Tungsten Halogen Lamp Adjustment Screw 30 until the light is centered on er 6 6 LAMP AND MIRROR ALIGNMENT f Press lt 630 gt GO TO A to set the wavelength to 630 Proper alignment of the lamps and mirror is essential for optimal performance of the spectrophotometer g Use the T A C key to put the instrument into Absorb CAUTION ance mode h Press lt 1 gt lt T A C gt and wait for the instrument to display an absorbance value It should be near 1 0 i Use a flatblade screwdriver with an insulated handle to turn the Tungsten Halogen Lamp Adjustment Screw 22 until the instrument displays a minimum absorbance reading If the instrument displays a negative absorbance reading stop and repeat step h j Use a Phillips screwdriver to turn t
20. EADING THIS WILL DEPEND ON DATA VALUES 510 REM EXPECTED AND SIGNAL AVERAGING SPEED 520 PRINT 5 SND REM ASK FOR THE DATA 530 INPUT 5 D X REM GET THE DATA 540 INPUT 5 AS REM AND WAIT FOR THE OK 550 PRINT D X REM OUTPUT THE DATA HERE T 560 LET X X 1 570 NEXT WV1 REM DO THE NEXT WAVELENGTH 22 335103 100018 APPENDIX C KEYBOARD LOCK MODE KLK TABLE OF ASCII CHARACTERS PRODUCED BY SPECTRONIC 501 601 KEYSTROKES CHARACTERS WILL ALSO BE PRODUCED FOLLOWING A CTL K KEY ASCII CHARACTER 0 9 0 9 lt CLEAR gt lt SEND gt 5 lt SECOND FUNCTION gt N lt DOWN gt D lt UP gt U lt T A C gt ib 335103 10001B INSIDE MAINTENANCE COVER 20 Lamp Interchange Mirror 21 Deuterium Lamp Adjustment Screw SPEC 601 22 Tungsten Halogen Lamp Adjustment Screw 23 Thumbscrew Deuterium Lamp removable 24 Deuterium Lamp SPEC 601 25 Deuterium Lamp Connector Wires SPEC 601 27 Monochromator Entry Slit not visible in drawing 28 Tungsten Halogen Lamp Socket 29 Tungsten Halogen Lamp Shield 30 Tungsten Halogen Lamp Vertical Adjustment Screw 31 Tungsten Halogen Lamp 32 Tungsten Halogen Lamp Shield Screw 2 26 Deuterium Lamp Assembly Screw fixed SPEC 601 TECHNICAL DATA Light sources Monochromator Detector Wavelength range Wavelength accuracy Wavelength precision Spectral slitwidth Stray radiant energy Photometric accuracy Photometric stability Noise Tungs
21. ION lt DEUT gt for the deuterium lamp or e SECOND FUNCTION gt lt TUNG gt for the tungsten lamp If the lamp was off it will be turned on if it was on it will be turned off The lamp status indicator the last display charac ter on the right of the LED display tells you which of the lamps is now activated T tungsten D deuterium or B both Another lamp selection option is STANDBY which turns 335103 100018 both lamps off temporarily To select the STANDBY feature enter 1 lt SECOND FUNCTION gt lt DIAG gt The lamps will remain off until you press any key resuming normal operation 4 6 LAMP INTERCHANGE WAVELENGTH lt LMP CH gt SPECTRONIC 601 ONLY You can set the wavelength at which the 501 601 switches between the tungsten halogen lamp and the deuterium lamp to any value between 320 and 380 nm The instrument auto matically switches to the deuterium lamp when the wave length is at or below the lamp interchange wavelength To select a new lamp interchange wavelength a Enter a wavelength value between 320 and 380 The dis play will read ENTRY followed by the value b Press lt SECOND FUNCTION gt The display will read 2ND c Press lt LMP CH gt The display will read LAMP CHANGE To display the current lamp interchange wavelength a Press lt SECOND FUNCTION gt The display will read 2ND b Press lt LMP CH gt The display will read LAMP CHANGE
22. LOG GAIN to n Outputs the current HIGH LIMIT Sets the HIGH LIMIT Turns the KEYBOARD LOCK mode ON n 1 or OFF n 0 Outputs the current LAMP CHANGE wavelength Sets the LAMP CHANGE wavelength Outputs the current LOW LIMIT Sets the LOW LIMIT Turns the LAMP SAVE mode ON n 1 or OFF n 0 Executes a POWER ON RESET Outputs the current SIGNAL AVERAGE value Sets the SIGNAL AVERAGE Outputs current wavelength data mode and data value per selected format Sets the TRANSMITTANCE Mode Turns the TUNGSTEN LAMP ON n 1 or OFF n 0 601 only Sets ABSORBANCE 0 CONCENTRATION 0 or TRANSMITTANCE 100 Outputs the data currently residing in the zero register in 8 character hexadecimal format Sets the zero register to n 2 APPENDIX B SAMPLE PROGRAMS 1 Obtain wavelength and absorbance data referenced to air of a sample at 775 nm FORMAT COMP ANS BACK ON 10 PRINT 5 ABS REM SET X01 TO ABSORBANCE MODE 20 PRINT 5 HIL 75 REM DISPLAY FLAG IS A gt 0 75 30 PRINT 5 LOL 70 REM DISPLAY FLAG IS A lt 0 70 40 PRINT 5 GTO775 REM CAUSE X01 TO GOTO 775 NM 50 INPUT 5 A REM WAIT FOR MESSAGE OK WHEN THE X01 IS AT 775 nm 60 PRINT 5 REM SET ABS TO ZERO WITH AIR IN THE SAMPLE BEAM 70 PRINT 5 SND REM CAUSE THE X01 TO OUTPUT THE DATUM OF THE SAMPLE IN THE SAMPLE BEAM 80 INPUT 5 D REM INPUT THE DATUM FROM THE X01 9
23. Milton Roy Service Set up external equipment properly or disconnect Turn off unit for 15 sec and turn on again If problem persists call Milton Roy Service Insert correct blank Increase sample volume Check sample preparation Go to correct wavelength Check deuterium peaks Check using SPECTRONIC Standards Call Milton Roy Service 6 4 2 Error Messages The chart below lists the error messages that the 501 601 will display The left hand column lists the error message as it RECOMMENDED ACTION OR ERROR MESSAGE PROBABLE CAUSE will appear on the 501 601 display the right hand column describes what to do if the message appears RECOMMENDED ACTION OR ERROR MESSAGE PROBABLE CAUSE NEED A NUMBER Re enter command sequence with appropriate number Re enter number entered in lt SECOND FUNCTION gt mode First entry did not correspond to any of the 501 601 s functions Check parameter table Section 5 2 Re enter correct parameter Re enter values and make sure that they are within the operating range of the spectrophotometer Replace the deuterium lamp see Section 6 5 2 RANGE 320 TO 380 Re enter lamp interchange wave length value Choose an appropri ate value between 320 and 380 nm Re enter signal average value Choose an appropriate value 0 1 or 2 The lamp you tried to turn off is required for operation at the cur rent wavelength Option or accessory selected has not been installed o
24. OR CHOPPER ERROR 195 999 NM ONLY 325 999 NM ONLY WARNINGS FOR LAMP REPLACEMENTS AND ALIGNMENT PROCEDURES HIGH VOLTAGE The deuterium lamp operates at extremely high voltage and has dangerous voltages on the terminals even when the lamp is turned off Be sure to turn power off and disconnect the power cord before you attempt to re place the lamp TEMPERATURE The tungsten lamp and the surrounding shield reach temperatures high enough to cause contact burns Make sure you allow the lamp to cool off for at least five minutes before removing the lamp 12 ULTRAVIOLET The short wavelength UV radiation emitted by the deuterium lamp can be harmful to the eyes and skin Wear eye protection and avoid exposing skin whenever the lamp cover is removed and the lamp is on for example when aligning the lamp mirror SHATTERING The tungsten lamp operates under high pres sure To avoid accidents protect the lamp from fingerprints abrasions and scratches when handling Wear eye protec tion when adjusting the lamp interchange mirror and never operate the lamp with the shield removed 335103 10001B 6 5 LAMP REPLACEMENT PROCEDURES This section contains instructions for replacing and aligning both the Tungsten Halogen and Deuterium lamps in the SPECTRONIC 501 and 601 spectrophotometers See inside back cover for illustrations It also contains instructions on several other maintenance procedures necessary from time to time k
25. SHOOTING 6 1 PERFORMANCE EVALUATION USING REFERENCE MATERIALS All precision instruments including spectrophotometers are subject to performance degradation These performance losses whether gradual or abrupt obvious or subtle can lessen the accuracy of analytical results Evaluation of your instrument s performance should start immediately with weekly checks for at least the first month At the end of this time monthly evaluations should be suffi cient to detect any gradual degradation of performance Evaluations should include tests for stray radiant energy wavelength accuracy photometric accuracy and photo metric linearity These tests involve the use of specialized test materials which must e Have values of known accuracy Be compatible with the instrument being evaluated and e Contribute minimum error to the test results Recommended test materials for this instrument are Milton Roy SPECTRONIC Standards Cat No 333150 These stan dards are designed to fit into the standard cuvette holder of the SPECTRONIC 501 or 601 spectrophotometer and can be used for the relevant tests For detailed instructions on the use of the standards refer to the SPECTRONIC Standards User s Manual Cat No 333150 10001 6 2 REPLACEMENT PARTS The following items are available as replacement parts for the SPECTRONIC 501 and 601 spectrophotometers CATALOG NO DESCRIPTION 335124 Tungsten halogen lamp box of 10 343424 Deuterium lamp 3351
26. WWW current wavelength S sign of data or space D DDD current data Location of decimal point depends on mode T A or C and data value X programmed output terminator Example bWWWbsD DDDX BILI UP 6 5 6 0 234 CRLF Response to all other parameter queries The spectropho tometer transmits information in either floating decimal point or integer format Only one variable will be output on any one line Example 1 234 100 567 1 9 2 COMMAND ENTRY Format of execute commands CCCbDDDDDDDDZZ CCC a three character command field b a space if data is to be sent DDDDDDDD a 0 to 8 character data field ZZ command terminator either a CR LE CR and LE or LF and CR Any of these are acceptable the terminator selected in the setup procedure is for output only 1 Some commands do not have a data field They merely consist of a three character command followed by a com mand terminator This type of command may either Ask the spectrophotometer to transmit a current data value or Set a specific operation 2 Commands that include a data field may initiate one of three possible functions Turn a specific spectrophotometer function on or off such as LSV 1 which turns the LAMP SAVE function on Enter a numerical value for a specific instrument param eter In this type of command 1 8 data characters may be entered These characters can include a decimal point and if applicable the nega
27. a tion value standard solution Make sure that spectrophotometer is in concen tration mode Place solution of known concentration into the sample compartment Enter appropriate value for the concentration value The display will read ENTRY followed by the value Press lt T A C gt e The display will read CALCULATE FACTOR then FACTOR followed by the new value The display will now return to the normal mode showing the wavelength and newly entered con centration values The concentration value that you enter will be divided by the current Absorbance value to calculate a new factor between 9999 and 9999 SECOND FUNCTION lt 100 T ZERO A gt sets transmittance to 100 or absorbance or concentration to zero 37 3 8 3 9 3 10 3 11 3 12 3 13 3 14 lt YES gt a Moves the monochromator up in wavelength b Causes the monochromator to go to a user specified wavelength Enter lt appropriate value gt Press lt YES gt The message GOING TO NM will be displayed until the monochromator is in the correct position Accepts prompted values that appear during test type programming requires Accessory Control Mod ule and APPLIPAK cartridge d Moves forward through a parameter list or EDIT list requires Accessory Control Module and APPLIPAK cartridge lt NO gt a Moves the monochromator down in wavelength b Causes the monochromator to go
28. acking list to ensure that you have received everything in good condition If any part is missing or damaged or you find any other defect please contact your dealer or Milton Roy sales representative OPERATIONAL CHECK a Set the unit on a clean lab bench or work area NOTE Take care to allow enough clearance behind the spec trophotometer for efficient operation of the exhaust fan b Check the voltage label plate on the spectrophotom eter s back panel to determine what voltage 100 120 220 or 240 has been set before you plug your SPECTRONIC 501 or 601 in If the voltage shown on the label plate is not the same as the voltage of your power source do not plug the instrument in call your authorized Milton Roy Sales Service represen tative to arrange for a voltage conversion c If the power outlet is satisfactory plug in the spec trophotometer s power cord d Turn power switch on e Check to make sure that the Power On Sequence operates properly SELF TEST message followed by GOING TO _ _ NM and then by the normal data display f Allow thirty minutes for warm up g Check lamp alignment by performing the Lamp Alignment Diagnostic Section 6 6 3 1 3 2 3 3 3 4 3 5 3 6 SECTION 3 KEYBOARD FUNCTIONS SECOND FUNCTION gt activates a key s second func tion The function or its abbreviation is printed just above the individual key SEND transmits data to t
29. e for the goods and or services for which liability is claimed FCC COMPLIANCE STATEMENT FOR U S A USERS This equipment generates uses and can radiate radio frequency energy and if not installed and used in accord ance with the instruction manual may cause interference to radio communications It has been tested and found to comply with the limits in effect at the time of manufacture for a Class A computing device pursuant to Subpart J of Part 15 of FCC Rules which are designed to provide rea sonable protection against such interference when oper ated in a commercial environment Operation of this equipment in a residential area is likely to cause inter ference in which case the user at his own expense will be required to take whatever measures may be required to correct the interference NOTE This operator s instruction manual contains information instructions and specifications that were believed accurate at the time this manual was written However as part of our on going program of product development the specifi cations and operating instructions may be modified or changed from time to time The Milton Roy Company reserves the right to change such operating instructions and specifications Under no circumstances shall Milton Roy be obligated to notify purchasers of any future changes in either this operator s instructions manual or any other instructions or specifications relating to the SPECTRONIC 501 601 and their accesso
30. e value A space is transmitted for a positive value D DDD datum value with a floating decimal point Data readings that exceed the range of the 501 601 0 1A to 2 999A will cause error messages UNFL data below 0 1A or OVFL data above 2 999A to be printed L high low limit flag If the current data value is not between the preset high and low limits an asterisk will be transmitted in this position Otherwise a space will be sent M data mode T TRANSMITTANCE A ABSORB ANCE or C CONCENTRATION X lamp ON OFF status D deuterium T tungsten B both Z output terminator CR LE CRLF or LFCR as specified by the firmware setup Example of Printer Output CWWWNMbSd DDDLMbXZ TREE TA EIL R50 0NM 0 029 A T CRLF Explanation This printout indicates that a cartridge test R is running For this test the spectrophotometer was set to 500 nanometers and 0 029 absorbance A was measured This data is not between the preset high and low limits The Tungsten T lamp is turned on 335103 10001B 9 1 2 Computer Format This format will be used by the instrument in response to a SND command see section 9 2 or when the 501 601 s SEND key is pressed and the firmware specifies the com puter format Data that exceeds the range of the instrument 0 1A to 2 999A will be sent as 9999 or 9999 Example bWWWbSD DDDX Where b space
31. eep your instrument operating at its peak 6 5 1 Tungsten Halogen Lamp CAUTION 1 The Tungsten Halogen lamp operates under high pressure To avoid accidents protect the lamp from fingerprints abrasions and scratches when han dling Wear eye protection when adjusting the lamp interchange mirror and never operate the lamp with the shield removed 2 Allow enough time for the shield and lamp to cool If the spectrophotometer has been operating even for a short time the shield and lamp will be very hot Temperatures are high enough to cause contact burns so allow at least 5 minutes before removing the shield and lamp a Turn the spectrophotometer off and disconnect the power cord b Remove the Maintenance Lamp Door by loosening the Lamp Maintenance Compartment Access Screw three to five complete turns and lifting up the door at the back edge c Remove the Tungsten Halogen Lamp Shield 29 from the instrument chassis by loosening the two Tungsten Halogen Lamp Shield Screws 32 The base of the shield is slotted as seen in Figure 6 1 so that you can slide it out from under the two screws and lift it straight up BE CAREFUL TO AVOID SCRATCHING THE LAMP INTERCHANGE MIR ROR 20 a Pull the 2 pin Tungsten Halogen Lamp 31 straight out from the socket CAUTION Use a clean lint free cloth or wear lint free gloves to handle the new lamp fingerprints on the lamp detract from its peak performance and s
32. fteen 15 days after delivery This warranty covers all parts except those specified below and applies only to equipment which has been installed and operated in accordance with the operator s instruction manual and which has been serviced only by authorized Milton Roy dealers or service personnel This warranty does not apply to equipment and accessories that have been damaged by accident neglect or condi tions beyond Milton Roy s control This warranty does not apply to lamps glassware and similar expendable components However such parts and components may be warranted by their manufacturer Milton Roy is not responsible under this warranty for loss in operating performance due to environmental conditions THIS WARRANTY IS IN LIEU OF ALL WARRANTIES EXPRESS IMPLIED OR STATUTORY INCLUDING BUT NOT LIMITED TO WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE OR OF MERCHANTABILITY OR OTHERWISE and states Milton Roy s entire and exclusive liability and the Customer s exclusive remedy for any claim in connection with the sale or furnishing of services goods or parts their design suitability for use installa tion or operations The Milton Roy Company will in no event be liable for any direct indirect special or con sequential damages whatsoever including loss of good will whether grounded in tort including negligence strict liability or contract and Milton Roy s liability under no circumstances will exceed the contract pric
33. functions and enters parameter data Tactile and audible response indi cates data entry 4 Lamp Save allows you to use the tungsten lamp at lower illumination level to extend lamp life 5 Lamp Indicator shows T when Tungsten Halogen lamp is on D when deuterium lamp is on and B when both lamps are on 6 16 character Alphanumeric Display shows parameters and data values as well as operating and error messages SAMPLE COMPARTMENT 7 Cell Holder Base is removable for installation of acces sory sample holders e g multipurpose cell holder ambi ent flowcell thermoelectric flowcell and automatic cell positioner 8 10mm pathlength Cuvette Holder is removable for instal lation of accessory cell holders e g thermal cell holders test tube holder and long pathlength cell holder 9 Filter Holder holds filters up to 7mm thick 10 Sample Compartment Windows 11 Capped hole available for installation of accessory plug connector BACK PANEL 12 Optional Recorder Interface connector 13 Sample compartment accessory connector 14 RS232 Interface connector used for computer interface printer and accessory control box 15 Power Switch 16 Line fuse 17 Power cord 18 Maintenance Lamp cover access screw 19 Exhaust fan 335103 10001B 335103 10001B SECTION 2 INSTALLATION 2 1 2 2 CONTENTS CHECK After carefully unpacking the contents check the mate rials against the p
34. gnostic 6 except that monochromator scans from 489 to 483nm Press CLEAR to exit PEAK NOISE MEASUREMENT 22 7 Measures noise for approximately 15 seconds and displays peak to peak noise in absorbance The value is computed on the displayed data and will apply the SIG gt value that was in effect before selecting this diagnostic Press CLEAR to exit USER DIAGNOSTICS DIAG 9 10 11 12 15 14 10 FUNCTION CLEAR AUTO ZERO REGISTER Undoes the AUTO ZERO function STRAY RADIANT ENERGY SRE MEASURE MENT Checks SRE by displaying transmittance to within 0 01 resolution To use this diagnostic move the spectrophotometer to the appropriate wavelength set 100 and insert the appro priate SRE filter Then enter 10 lt SPECIAL FUNCTION gt lt DIAG gt The SRE measurement will be displayed in the form SRE XX XX T and will be held for 0 5 seconds for a longer display depress and hold down any key as long as you wish to see the display SERIAL PORT SETUP Prompts the operator through the setup of the RS 232 C serial port parameters baud rate parity etc Refer to Section 9 of this manual for a complete explanation READ INSTRUMENT ID CODE Displays the spectrophotometer s identification code for use with Milton Roy disk based software programs LAMP ALIGNMENT Prompts for lamp choice SPECTRONIC 601 only moves to appropriate wavelength then converts the display
35. he Tungsten Halogen Lamp Vertical Adjustment Screw 30 until the instrument displays a minimum absorbance reading k Repeat step i The alignment itself consists of two parts L 601 only Press 240 5 lt GO TO A gt to set the wave Adjusting the position of the light beam hitting and passing length to 240 nm through the filters Minimizing the absorbance reading The position of the mirror determines the amount of light hitting and passing through the slit The amount of light in turn directly affects your results and the overall performance of the spectrophotometer These steps help ensure peak per 1 The instrument uses high voltage and dangerous voltages may be present even when the lamp is turned off Use insulated tools to avoid danger of electrical shock and perform adjustments with ex treme caution 2 The short wavelength radiation emitted by the deu terium lamp can be harmful to the eyes and skin Wear eye protection and avoid exposing skin for more than a few minutes when the lamp cover is removed and the lamp is on m 601 only Use the T A C key to put the instrument into Absorbance mode n 601 only Press lt 1 gt lt T A C gt and wait for the instrument to display an absorbance value It should be near 1 0 formance of your instrument a If the instrument is off turn it on and wait for it to initialize If it is on turn if off and then on again so that it initializes correctly
36. he optional serial port accessory For a more detailed description refer to Sections 7 through 10 of this manual If the optional printer or an external computer is connected to the RS 232C output on the rear panel of the instrument Press lt SEND gt The data on the display will be transmitted to the computer or printer SECOND FUNCTION lt SELECT gt allows you to load a stored test requires the Accessory Control Mod ule and an APPLIPAK cartridge CLEAR gt restores a normal data display and is used to exit several diagnostic tests lt T A C gt a Changes the data mode e Press lt T A C gt to switch from Absorbance to Transmittance mode e Press lt T A C gt again to enter the Concen tration mode Press lt T A C gt to return to the Absorbance mode b Can be used to enter an absorbance value Make sure that the spectrophotometer is in the absorbance mode then e Enter lt appropriate value gt e Press lt T A C gt Display will read NEW ABS ZERO e Display will show the wavelength and new absorb ance value The absorbance value that vou enter will take the place of anv previous value The difference between the new value and the previous value is produced bv adjusting or offsetting from zero e g if vour pre vious ABS value was 0 92 and you entered 0 97 the value of zero will be adjusted or offset to 0 05 c Calculates a factor when you enter a concentr
37. he parameters to the desired status or value e Press lt 1 gt then 47 The display will read ENTRY 14 e Press lt SECOND FUNCTION gt The display will read 2ND 14 Press lt DIAG gt The display will read SETUP SAVED and then return to a normal data display These values will now be the default values that will be present the next time you turn on your spectrophotometer even if you enter different parameters into current memory before you turn the unit off The only way to alter these default or power up values is to perform another Test Parameter Storage procedure as described above but with new values Table 5 1 Parameters Acceptable Values RANGE OF ACCEPTABLE PARAMETER FUNCTION VALUES Wavelength Wavelength to nearest nanometer 195 999nm 601 WV 325 999nm 501 Low Limit Lowest acceptable final answer 0 0000 to 9999 LO LIM gt If display is below this value appears High Limit Highest acceptable final answer lt HILIM gt If display is above this value appears Signal Average Data enhancement technique to lt SIG AV gt reduce background noise Lamp Change Wavelength at which instrument LMP CH switches between tungsten halogen and deuterium lamps Factor Multiplier used to convert absorbance value to a concentration level 0 0000 to 9999 0 to 2 whole numbers only 320 to 380 nm 0 0000 to 9999 335103 10001B SECTION 6 MAINTENANCE amp TROUBLE
38. horten the life of the lamp e Turn the instrument 90 degrees to locate the lamp socket more easily f Carefully align the pins on the new lamp with the socket Figure 6 2 g Slowly push the lamp straight into the socket until fully seated be sure that the lamp is straight to avoid bending the pins h Carefully remove any fingerprints or dirt from the lamp with a clean lint free cloth i Carefully replace the shield and secure it with the two screws loosened in step c j Perform the alignment procedure described in Section 6 6 Lamp and Mirror Alignment before using the instrument 6 5 2 Deuterium Lamp SPECTRONIC 601 only CAUTION The deuterium lamp operates at an extremely high voltage and has dangerous voltages on the terminals even when the lamp is turned off Be sure to turn off the power and disconnect the power cord before you attempt to replace the lamp 335103 10001B Lamp a Turn off the spectrophotometer and unplug the power cord b Remove the Maintenance Lamp Access door by loosening the Lamp Maintenance Compartment Access Screw three to five complete turns c Disconnect the three Deuterium Lamp Connector Wires 25 from the sockets d Loosen but do not remove the Deuterium Lamp Assembly Screw 26 e Remove the Thumbscrew 23 holding the Deuterium Lamp Assembly in place and remove the old lamp assembly f Connect the new
39. nt setting The LMP SAV feature reduces the level of illumination about 50 at wavelengths below 400 nm and about 25 at wave lengths above 400 nm while increasing average lamp life expectancy an average of 40096 Of course the choice of when to use this feature will depend on a number of condi tions but in general LMP SAV should be satisfactory for most measurements between 400 and 900 nm if you are using a full aperture cuvette or test tube The SPECTRONIC 501 and 601 spectrophotometer can dis play the following error message to indicate tungsten lamp problems If the lamp s output has decreased due to aging or if the lamp has burned out the message TOO LITTLE LIGHT will appear during the AUTO ZERO operation see Section 6 5 1 335103 100018 4 4 DEUTERIUM LAMP SPECTRONIC 601 ONLY The SPECTRONIC 601 spectrophotometer displays two types or error messages to indicate deuterium lamp problems If the light output has been reduced by aging or if the lamp has burned out the message TOO LITTLE LIGHT will appear during the AUTO ZERO operation e If the deuterium lamp fails to turn on within 15 seconds the message D2 LAMP FAILURE will appear on the dis play See Section 6 5 2 Lamp Replacement Procedures Deuterium Lamp 4 5 LAMP CONTROL SPECTRONIC 601 ONLY On the SPECTRONIC 601 spectrophotometer you can turn the deuterium or tungsten halogen lamps on or off by entering e SECOND FUNCT
40. nting the characters A through Z and 0 through 9 To stop the test press any key after s and 0 5 have been displayed RECORDER SETUP routine provides three op tions for recorder adjustment but will run only if the Recorder Output Option has been installed When you press 4 SECOND FUNCTION gt lt DIAG gt the message RECORDER SETUP will appear first You may now select any of the setup options below by pressing 0 1 or 2 If you press 0 The message RECORDER ZERO will be dis played to indicate that analog output has been set to zero Press lt CLEAR gt to exit If you press 1 The message RECORDER 1 UNIT will appear and the analog output will be set to 1 volt for recorder adjustment This corresponds to 1A or 100 T Press lt CLEAR gt to exit If you press 2 The message RECRDR OUT TEST will appear and the analog output will be set at the lowest voltage approximately 0 3vdc for three sec onds The voltage level will then then ramp up in about 4 seconds to the highest voltage ap proximately 3 7 vdc holding at that voltage for 3 seconds before returning Press lt CLEAR to exit 656NM DEUTERIUM PEAK FIND 601 ONLY This routine turns on the Deuterium lamp rotates the mirror and sets the appropriate gain It also moves the monochromator from 659 to 653nm and displays the measured peak wave length Press lt CLEAR to exit 486NM DEUTERIUM PEAK FIND 601 ONLY This routine is similar to dia
41. pecified range will be flagged an asterisk will appear in the fourth display position from the right NOTE The flag appears when the instrument measures values outside the range that you have previously specified An other set of flags are used to indicate that the instrument is measuring values that exceed the range that the spec trophotometer can accommodate Errors of this type out of range value are displayed with the message OVFL or see Section 6 4 2 Error Messages To enter values for the limits a Press the desired value between 0 0000 and 9999 The display will read ENTRY followed by the value b Press SECOND FUNCTION gt The display will read 2ND followed by the value c Press lt LO LIM gt The display will read LO LIM d Press the desired value between 0 0000 and 9999 The display will read ENTRY followed by the value e Press SECOND FUNCTION The display will read 2ND followed by the value f Press HI gt The display will read HI LIM To display the current HI LIM and LO LIM values a Press SECOND FUNCTION gt The display will read 2ND b Press LO LIM gt The display will read LO LIM c Press SECOND FUNCTION The display will read 2ND d Press HI gt The display will read HI LIM 4 2 SIGNAL AVERAGING lt 516 gt Signal averaging is a form of electronic filtering which re duces the backg
42. r CPU has not been instructed that option has been installed If accessory has been installed and error persists call Milton Roy service Illumination level may be too low If LAMP SAVE feature is on turn it off May also occur if path of light beam in sample compartment is NO FUNCTION ILLEGAL ENTRY OUT OF RANGE D2 LAMP FAILURE SIG AV 01 OR2 LAMP REQUIRED OPTION REQUIRED TOO LITTLE LIGHT disturbed or if the sample has too high an absorbance at the selected wavelength try diluting sample or moving to another wavelength CALIBRATION ERROR Perform lamp alignment procedures Section 6 6 If error persists call Milton Roy service Check maintenance compartment for obvious obstructions or damage Verify that instrument has been set up to run at the correct voltage level If error persists call Milton Roy service Check maintenance compartment for obvious obstructions or damage Verify that instrument has been set up to run at the correct voltage level If error persists call Milton Roy service Check maintenance compartment for obvious obstructions or damage Verify that instrument has been set up to run at the correct voltage level If error persists call Milton Roy service Re enter wavelength value and make sure that it is between 195 nm and 999 nm SPECTRONIC 601 only Re enter wavelength value and make sure that it is between 325 nm and 999 nm SPECTRONIC 501 only FILTER ERROR MONO ERR
43. ries nor shall Milton Roy be liable in any way for its failure to notify purchasers of such changes Copyright 1985 1988 by Milton Roy Company All rights reserved SPECTRONIC and are registered trademarks of Milton Roy Company i 335103 100018 CONTENTS SECTION 1 2 TITLE PAGE OPERATOR FEATURES 2 INSTALLATION 3 2 1 Contents Check 2 2 Operational Check KEYBOARD FUNCTIONS 4 DETAILED FEATURE DESCRIPTION 6 4 1 High and Low Limits 4 2 Signal Averaging 4 3 Tungsten Lamp 4 4 Lamp Selection 601 only 4 5 Deuterium Lamp 601 only 4 6 Lamp Interchange Wavelength 601 only OPERATION 8 5 1 Basic Spectrophotometric Measurements 5 2 Test Parameter Storage MAINTENANCE amp TROUBLESHOOTING 9 6 1 Performance Evaluation 6 2 Replacement Parts 6 3 Diagnostics 64 Maintenance and Troubleshooting 6 4 1 Troubleshooting Chart 6 4 2 Error Messages 6 5 Lamp Replacement 6 5 1 Tungsten Halogen Lamp 6 5 2 Deuterium Lamp 601 only 6 6 Lamp and Mirror Alignment 6 7 Fuse Replacement RS 232 C INTERFACE INTRODUCTION 15 RS 232 C FIRMWARE SETUP PROCEDURE 17 RS 232 C PROGRAMMING GUIDELINES 19 APPENDIX A ALPHABETICAL LIST OF COMMANDS 21 APPENDIX B PROGRAM EXAMPLES 22 APPENDIX C KEYBOARD CODES 23 SECTION 1 OPERATOR FEATURES FRONT 1 Sample Compartment Door 2 Maintenance Lamp Access Door provides access for lamp replacement and alignment 3 Sealed Keyboard controls instrument
44. round noise in the system as illustrated in Figure 3 1 This feature is particularly useful at higher ab sorbance levels above 2A and at wavelength extremes near 195 nm or 999 nm The SPECTRONIC 501 and 601 provide three levels of signal averaging from 0 minimum filtering to 2 maximum filtering To change the SIG AV value a Enter an integral value of 0 1 or 2 The display will read ENTRY followed by the value b Press SECOND FUNCTION gt The display will read 2ND followed by the value c Press SIG gt The display will read SIG AV To display the current SIG AV value a Press SECOND FUNCTION The display will read 2ND followed by the value b Press SIG AV gt The display will read SIG AV Po ipu Pss adl SIG AV 1 mud SOLO SS ERG SIGAV 2 Figure 3 1 Typical Effects of Signal Averaging 4 3 TUNGSTEN LAMP The tungsten halogen lamp used in both the SPECTRONIC 501 and 601 spectrophotometers can be set to two levels of illumination through the use of the LAMP SAVER LMP SAV feature This feature allows the option of extending lamp life when maximum illumination is not required e 0 SECOND FUNCTION LMP SAV gt turns the lamp save function off so the lamp is on at full intensity e 1 SECOND FUNCTION lt LMP SAV gt turns the lamp save function on so the lamp operates at reduced intensity SECOND FUNCTION gt lt LMP SAV gt displays the cur re
45. t a known factor for use with the concentration data mode Enter appropriate value The display will read ENTRY followed by the value Press SECOND FUNCTION gt The display will read 2ND followed by the value Press lt FACTOR gt This value will be used to con vert absorbance data into concentration units 335103 100018 35 3 16 3 17 3 18 319 b Display the current factor e Press lt SECOND FUNCTION gt The display will read 2ND e Press lt FACTOR gt SECOND FUNCTION HI LIM gt displays or sets the highest acceptable final result Any result above the limit will be flagged as out of limits see Section 4 1 High and Low Limits SECOND FUNCTION gt lt LO LIM gt displays or sets the lowest acceptable final result Any result below the limit will be flagged as out of limits see Section 4 1 High and Low Limits SECOND FUNCTION gt lt DIAG gt accesses the inter nal diagnostics See Section 6 3 Diagnostics SECOND FUNCTION gt SIG AV gt displays or sets the signal averaging value see Section 4 2 Signal Averaging SECOND FUNCTION gt LMP SAV gt turns the tung sten lamp save on and off see Section 4 3 Tungsten Lamp e Enter 0 lt SECOND FUNCTION LMP SAV gt to turn off the lamp save mode e Enter 1 SECOND FUNCTION gt LMP SAV gt to turn on the lamp save mode e Press lt SECOND FUNCTION gt LMP gt to dis play the status
46. ten Halogen 325 to 999nm Deuterium 195 to 380nm SP 601 only Modified Czerny Turner with holographic grating 1200 lines per millimeter Single silicon photodiode 501 325 999nm 601 195 999nm 1 0nm Better than 0 5nm lt 5nm 0 05 T at 220nm and 340nm 0 005 A near 1 0A 0 003 A per hour near 0 A Less than 0 004 A near 2A nm Less than 0 0015 A near 0A Photometric Ranges Sample compartment dimensions Power requirements Overall dimensions Weight Environment Operating Storage Absorbance 0 1 to 3 0 A Transmittance 0 0 to 125 T concentration 9999 to 9999 10 2 cm 4 in High x 10 6 cm 4 2 in Wide x 17 1 cm 6 5 in Deep 100 115 and 220 240 V 10 50 60 Hz 2 Hz 2 0 A 19 7 cm H x 44 5 cm W x 49 cm DUIS Hx Veo Wx 14 3 D 15 kg 35 Ibs 50 105 F 10 40 5 C relative humidity 10 60 40 to 105 F 40 to 40 5 C relative humidity not to exceed 6096 Allow unit to adjust to room temperature for 24 hours when taking it out of storage Lamp Maintenance Compartment Acess Screw MILTON ROY Milton Roy Analytical Products Division E Vlietinckstraat 20 8400 Oostende BELGIUM Telephone 59 50 89 92 Milton Roy U K Ltd Milton Roy House Stone Business Park Staffs ST15 OHH ENGLAND Telephone 0785 81 35 42 Milton Roy Analytical Products Division 15 Rue Guyton De Morveau 75013 Paris FRANCE Telephone 1 45 88 28 00 Milton Ro
47. tive sign As previously stated if no sign is entered in the command the value is assumed to be positive Set a specific analytical mode of operation and enter a numerical value for that mode 17 9 3 COMMAND TABLE All of the valid commands are listed in this section Some of the 3 character commands are followed by an n which represents a variable data field TYPE OF COMMAND COMMAND DATA MODE ABS ABS CON CON TRN DATA ZER CONTROL FAC FAC n LOL LOLN HIL HIL n SIG SIG n OF COMMAND COMMAND COMMUNICATIONS SND KLK n DMD MONOCHROMATOR GTO n CONTROL LAMP DEUn TUN n LCH LCH n LSV n UTILITY HDG HDG n 18 ACTION CAUSED BY THIS COMMAND Sets the ABSORBANCE mode Sets the ABSORBANCE mode and ABSORBANCE value n n 0 to 2 999 Sets the CONCENTRATION mode Sets the CONCENTRATION mode and CONCENTRATION value n by calculating a FACTOR n 0 to 9999 Sets the TRANSMITTANCE Mode No numerical data permitted Sets the following zero values ABSORBANCE 0 CONCENTRATION 0 or TRANSMITTANCE 100 Outputs the current FACTOR value Sets the FACTOR to n Sign and decimal point may be included n 0 to 9999 Outputs the current LOW LIMIT value Sets the LOW LIMIT to n Sign and decimal point may be included n 0 to 9999 Outputs the current HIGH LIMIT value Sets the HIGH LIMIT to n Sign and decimal point may be included n 0 to
48. to a linear responding bar graph The left hand digit of the display indicates the relative gain achieved Numbers are dis played in hexadecimal format 0 through 9 and A through F with 0 being the lowest and F the highest lamp intensity See Section 6 6 POWER ON INSTRUMENT SETUP This diagnostic stores the current setup of the instrument The next time that the instrument is DIAG 15 16 FUNCTION turned on these parameter values will be present Parameters saved as part of this pro cedure include a current wavelength b lamp status D2 on off T on off Lamp Save on off c lamp change wavelength d data made T A C e factor C multiplier f signal averaging value g high low limits h RS 232 setup baud rate number of stop bits parity etc KEYBOARD TEST Simply verifies that each key is working Press the key and observe the display if keystroke does not produce the character listed in the chart below contact your authorized Milton Roy ser vice representative To exit from this test turn the spectrophotometer off and then on again KEY DISPLAYED AS CLEAR C SEND S SECOND FUNCTION gt N DOWN ARROW D UP ARROW gt U lt T A C gt a lt gt lt gt lt 0 9 gt NUMBER PRESSED PRINTER TEST Checks printer operation by transmitting ASCII codes for SPECTRONIC 501 601 S character set Operates continuously until you press lt CLEAR gt to exit
49. to a user specified wavelength Press lt appropriate value gt Press lt NO gt The message GOING TO NM is displayed until the monochromator is in the cor rect position c Rejects prompted values that appear during test type programming test type requires Accessory Control Module and APPLIPAK cartridge d Move backward through a parameter list or EDIT list requires Accessory Control Module and APPLIPAK cartridge SECOND FUNCTION GO TO A causes the monochromator to go to a user specified wavelength Enter appropriate value gt Display will show EN TRY value e Press lt SECOND FUNCTION gt The display will now read 2ND followed by the value Press GO TO A The message GOING TO NM will be displayed until the monochromator is in the correct position lt gt lt gt and 0 through lt 9 gt are used to enter positive and negative decimal and integer values SECOND FUNCTION EDIT changes any of the prompted parameters of the current test requires Accessory Control Module and APPLIPAK cartridge SECOND FUNCTION LIST displays the para meters and their values for the current test requires the Accessory Control Module and APPLIPAK car tridge SECOND FUNCTION gt lt CATALOG lists the cata log numbers and names of user stored tests requires the Accessory Control Module and an APPLIPAK car tridge SECOND FUNCTION gt FACTOR a Se
50. tometer b A cable assembly An 11 pin MOLEX Connector internally SIGNAL NAME PROTECTIVE GROUND TRANSMITTED DATA TD RECEIVED DATA RD REQUEST TO SEND RTS CLEAR TO SEND CTS SIGNAL GROUND DATA TERMINAL READY DTR 335103 10001B connects the cable to the circuit board The wires in the cable are connected to a DB 25P male connector which is mounted on the rear panel of the spectrophotometer This connector serves as the serial port for external commu nications c A 25 pin RS 232 C cable which is installed between the serial port and the external electronic device Output pin assignments are shown below FUNCTION Grounds the chassis Data output from the 501 601 Data input from the external device 12V from the 501 601 Used for handshaking Requires 3 25V from the external device or CTS line may be left open Return for Data amp Control signals Used for handshaking The 501 601 supplies 12V when power is on and the instrument is ready to accept data 15 SECTION 8 FIRMWARE SETUP PROCEDURE Before you can use the RS 232 C Interface you must first prepare it to communicate with an external device Follow the steps below to enter a specification for each RS 232 C firmware function Step 1 Enter 11 then press the lt SECOND FUNCTION gt and lt DIAG gt keys on the spectrophotometer keyboard The RS 232 C firmware functions will appear sequentially on the display To accept a
51. y Analytical Products Division M nchner Strasse 72 8043 Unterf hring WEST GERMANY Telephone 089 9501 51 Milton Roy Analytical Products Division Jahnstrasse 22 24 6467 Hasselroth 2 WEST GERMANY Telephone 0 60 55 33 66 Milton Roy Hong Kong Ltd Room 808 Kornhill Metro Tower One Kornhill Road Quarry Bay HONG KONG Telex 780 77401 Milton Roy KK Takanawa Daiichi Building 2 21 41 Takanawa Minato Ku Tokyo 108 JAPAN Telex 7810 242 4296 MRCTYO J 820 Linden Avenue Rochester 14625 Telephone 716 248 4000 Telex 4441037 MROYUI 335103 10001 8 0096 4 88 1MWL Printed in U S A Analytical Products Division MILTON ROY 820 Linden Avenue Rochester New York 14625 Telephone 716 248 4000 Telex 444 1037 Fax 716 248 4014 Dear SPECTRONIC Spectrophotometer Owner Congratulations on the purchase of your new SPECTRONIC spectro photometer You ve just become part of an instrumentation tradition that began in 1952 with the SPECTRONIC 20 the world s most widely used spectrophotometer The SPECTRONIC name is recognized and respected the world over as a symbol of technical excellence Originated by Bausch amp Lomb the SPECTRONIC tradition is now being carried on by the Milton Roy Company On April 8 1985 the Milton Roy Company acquired Bausch amp Lomb s Analytical Products Division the group that manufactures SPECTRONIC instruments The only thing that has changed is our name our commitment to
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