Gradifrac specification
Contents
1. 6 1 The display of the GradiFrac System should show Main Menu RUN METHOD Press the down arrow until Main Menu MANUAL RUN is showing Press ENTER 6 1 6 2 Press the down arrow until Manual Flow mL min is displayed Enter the desired flow rate as specified in the appropriate manufacturing document e g 5 0 Press ENTER The pump will now start drawing the liquid 6 1 6 3 The display now shows MANUAL FRACTION mL Press ENTER and enter the desired volume e g 2 0 when it is time to start collecting Everything should now be working If drops of liquid do not begin to flow into the first collection tube press END The display will read Main Menu RUN METHOD Repeat section 6 1 until desired material has been eluted Documentation The user shall complete the GradiFrac System Log ES1704 1 If a new log sheet is required the user shall record the number of hours run since the last tubing replacement If the Pump P 1 tubing has just been replaced the number of hours run shall be recorded as zero Forward completed log sheets to QC for review File log sheets in the EQ file EQ 064 Maintenance 6 2 1 6 2 2 6 2 3 The GradiFrac should be cleaned thoroughly after each use The surface may be wiped with aqueous cleaning solutions or with ethanol The delivery arm should always be returned to the central position over the bowl when not in use Document the cleaning on ES1704 1 Tubing on the peris2. EQUIPMENT SPECIFICATION Page 1 of 4 Title GRADIFRAC SYSTEM Document Number ES1704 Supersedes Date 2000 11 20 CO No 10 0454 Revision 02 QA Approval Date Dan Simpson 2010 08 04 Effective Date 2010 08 06 1 INTRODUCTION This document describes the proper use and maintenance of the GradiFrac System EQ 064 and its associated columns with flow adaptor EQ 065 The GradiFrac System from Pharmacia Biotech AB combines the functions of gradient programmer and programmable fraction collection for standard chromatography applications The GradiFrac System is composed of several components The GradiFrac instrument can be programmed to collect fractions based on time volume drop count or in a peak fraction mode when the monitor is in use The Pump P 1 is a single channel laboratory pump for use in liquid chromatography for accurately controlled liquid flows The Monitor UV 1 is a fixed wavelength monitor to measure the absorbance of a flowing liquid The Recorder REC 102 allows charting during fraction collections The Valve IV 7 Switch Valve PSV 50 and Chromatography Column with Flow Adaptor are also components This document does not address preparing regenerating or storing columns This information is covered in the appropriate manufacturing documents RESPONSIBILITY The user shall be responsible for the proper use of the GradiFrac System The department in which the instrument resides shall be responsible for its maintenanc
3. Revision Description cCoO Effective Date 02 Removed O ring replacement Removed run rate and fraction columns from log 10 0454 2010 08 06 GRADIFRAC SYSTEM LOG Number of hours run since last tubing replacement Date User Cleaned 1 Peristaltic Pump P 1 Tubing Total number of hours run Date Technician If the total number of hours run is 200 replace the tubing Tubing Replaced Yes No QC Review Date Controlled Document ES1704 1 02 Page 1 of 1 Effective 2010 08 06
4. e REFERENCES GradiFrac System Installation Guide Pharmacia Biotech publication number 18 1035 18 Edition AD 1994 GradiFrac User Manual Pharmacia Biotech publication number 56 1006 08 Edition AC 1994 Monitor UV 1 User Manual Pharmacia Biotech publication number 59 7797 01 Edition AF 1994 GradiFrac System with HiLoad Pump P 50 Installation Guide Pharmacia Biotech publication number 18 1103 53 Edition AA 1994 Peristaltic Pump P 1 User Manual Pharmacia Biotech publication number 59 4698 01 Edition AG 1995 Recorder REC 101 102 User Manual Pharmacia Biotech publication number 80 1300 59 Edition AE 1994 Columns C10 C16 C26 Instructions Pharmacia Biotech publication number 59 5261 00 Edition AH Adaptors AC 10 AC 16 AC 26 Instructions Pharmacia Biotech publication number 56 1021 49 Edition AD Solenoid Valve PSV 50 Instructions Pharmacia Biotech publication number 19 1994 05 Edition AD 1995 Valve IV 7 Instructions Pharmacia Biotech publication number 19 1996 05 Edition AC 1993 SAFETY Always disconnect all instruments from their power source before performing any maintenance EQUIPMENT SPECIFICATION Page 2 of 4 Title GRADIFRAC SYSTEM Document Number ES1704 Revision 02 Effective Date 2010 08 06 5 EQUIPMENT MATERIALS Kimax 51 borosilicate disposable glass culture tubes 18 X 150 mm VWR 60825 673 or equivalent 6 PROCEDURE Note To use the GradiFrac system you must have a packed column Ref
5. er to the appropriate manufacturing document 6 1 Operation 6 1 1 Monitor UV 1 6 1 1 1 Turn the Control Unit on 6 1 1 2 Select the desired Absorbance range as specified in the appropriate manufacturing document 6 1 1 3 Make sure that the mode switch is in the AU mode 6 1 2 Connecting the Chromatography Column 6 1 2 1 Connect the chromatography column between port 1 of the IV 7 valve and the lower left port of the UV 1 cell flow 6 1 2 2 Place the A tubing coming from the PSV 50 valve in the solution to be run through the column 6 1 3 Recorder 6 1 3 1 Install a colored pen by gently pushing it into the lower metal pen holder 6 1 3 2 Select the desired chart speed with the chart speed switch as specified in the appropriate manufacturing document 6 1 3 3 Make sure that the Rec off on key is in the down position The recorder will then start when the flow is started and stop when the flow is stopped 6 1 4 Pump P 1 Make sure that the pump is on The pump will start when the flow is started and stop when the flow is stopped EQUIPMENT SPECIFICATION Page 3 of 4 Title GRADIFRAC SYSTEM Document Number ES1704 6 2 6 1 7 Revision 02 Effective Date 2010 08 06 Fraction Collector 6 1 5 1 Move the delivery arm up and out 6 1 5 2 Insert the collection tubes in the tube rack 6 1 5 3 Lift the delivery arm a little and move it so that the tube sensor rests against a tube Running the system 6 1
6. taltic pump P 1 should be replaced after 200 hours to minimize the risk of tube breakage during operations Document tubing replacement on ES1704 1 To ensure trouble free operation of the monitor the following precautions should be taken 6 2 3 1 All liquids through the flow cell should be free of suspended particles 6 2 3 2 Never allow buffer solutions to dry out in the flow cell EQUIPMENT SPECIFICATION Page 4 of 4 Title GRADIFRAC SYSTEM Document Number ES1704 Revision 02 Effective Date 2010 08 06 6 2 4 6 2 5 6 2 3 3 All liquids should be degassed to prevent air bubble formation in the flow cell 6 2 3 4 Always rinse the flow cell thoroughly with distilled water after use 6 2 3 5 Never touch the optical surfaces of the interference filters or expose them to temperatures greater than or equal to 60 C The recorder requires minimum maintenance No lubrication is necessary Never lubricate the guide axis of the pen carriage Clean the guide axis periodically with a soft cloth to remove dust When changing the chart paper remove any excessive dust When finished with a run reconnect tubing between port 1 of the IV 7 valve and the UV 1 flow cell This creates a closed system to prevent dust in the GradiFrac 6 3 Troubleshooting Reference the operation manuals for each specific component part for troubleshooting suggestions 7 ATTACHMENTS APPENDICES GradiFrac System Log ES1704 1 REVISION HISTORY
Download Pdf Manuals
Related Search